WO2014199388A1

WO2014199388A1 - Mosquito killing device

Info

Publication number: WO2014199388A1
Application number: PCT/IN2013/000465
Authority: WO
Inventors: Loganathan KUMAR
Original assignee: Muktha Laboratories Private Limited
Priority date: 2013-06-12
Filing date: 2013-07-25
Publication date: 2014-12-18
Also published as: HK1208306A1; CN104427866A

Abstract

This invention discloses a method, a composition and a device for reducing the disease transmission caused by pests and specifically mosquitoes. The component is a combination of 2 preparations, of which one preparation shall have olfactory attractive index in the range of 0.3 to 0.4 and the second preparation shall have olfactory attractive index in the range grater than 0.47. The method involves similarly human masking and attracting mosquitoes into a target area. The device comprises to generate the target area for attracting the mosquitoes and killing the same by asphyxiation.

Description

TITLE: MOSQUITO KILLING DEVICE

BACKGROUND OF INVENTION

MUST-QUIT-O is a trapping device designed to kill mosquitoes and can be used for both domestic and enterprise purposes. The innovative characteristics of the device are the chemicals' ability to simultaneously mask humans & attract mosquitoes; and the device design. Mosquitoes are insects that have been around for more than 30 million years. There are over 3,500 species of mosquito throughout the world. But it is only the female of the species which bites as she searches out nourishment for her eggs. Mosquitoes find their human prey using a combination of sensory signals including light, shape, color, heat, movement and other by products of human activity. They are known to cause harmful diseases like Malaria, Chikungunya and Dengue by transmitting disease causing pathogens (P. falsiparum) into humans. Every year millions die due to Malaria and the worst affected are children below the age of 5 (Source: WHO).

Most of the products currently present in the international market (e.g. Mosquito magnet, Dynatrap, Mega-Catch etc.) are based only on the attraction principle and those in the Indian market (Allout, Good knight, Mortein etc.) work by nullifying or paralyzing mosquitoes and repelling them. Products like Mosquito magnet, Dynatrap and Mega-Catch are expensive, bulky and not highly effective. Moreover, Dynatrap is an outdoor device which is not highly effective when used indoors. Domestic products are known to cause allergies, headaches and other side-effects and also these products do not eliminate mosquitoes from the area.

OBJECT OF THE INVENTION

The above mentioned disadvantages have been overcome in the current device. Since, MUST-QUIT-O is a mosquito killing device that simultaneously masks and attracts; it eliminates mosquito populations from areas of human inhabitation. For

l every mosquito killed, 3000 eggs are also eliminated. Killing takes place by a suction mechanism due to which the mosquitoes get trapped in the device; followed by asphyxiation (dehydration).

The chemical formula consists of Hexadecanoic acid, Diethylenediamine, Butylated Hydroxy Toluene (BHT), Isopropanol & Liquid Paraffin or Ionized water. Hexadecanoic acid & Diethylenediamine is used for blocking the olfactory sensors of the mosquitoes in the Olfactory Attractive Index (OAI) range of 0.3 to 0.4 which is the OAI range of Human beings. Hence humans are masked from the mosquitoes & the mosquitoes are attracted towards the device which has the OAI range >0.47. In the above chemical formulation, BHT & Isopropanol creates OAI value of above 0.47 which attracts mosquitoes.

DETAILED DESCRIPTION OF THE INVENTION:

Best mode of performing the invention

The best mode of performing the invention is using either of the two chemical formulations given below (discussed in detail as examples 3 and 4 respectively) in the device that has the optimized design (discussed below):

Hexadecanoic acid (from 0.08% w/w to 0.15%w/w)

Diethylenediamine (fromO.26% w/w to 0.73%w/w)

BHT 1%w/w

Isopropanol 1%w/w

Perfume 1% w/w [Drakkar/Cresol Based Perfume]

Liquid Paraffin or Ionized water 96.12% w/w

Hexadecanoic acid (from0.08%w/w to 0.15% w/w)

Thiomorpholine, 4-methyl- (from 0.26% w/w to 0.73%w/w) BHT 1%w/w

Isopropanol 1 %w/w

Perfume 1.1 w/w (M 1998/ Lavender/ Cresol Based Perfume) • Liquid Paraffin or Ionized water 95.40%w/w

An important lead to develop the chemical formulation was the knowledge that OAI<- 0.3 would work as a repellent, OAI from -0.3 to + 0.3 would have no effect and OAI> 0.3 would attract mosquitoes. Hence, it was necessary that our device had an OAI> 0.3. Initial experiments were carried out with cresol as cresol and human sweat have the same or greater OAI. But, cresol was toxic when used in quantities > 5ppm. Hence, various other related chemicals were tested and the constituents' ratios were varied, to arrive at the current chemical formulation, which has an OAI> 0.55.

Above two formulas OAI value> 0.47 and masking effect 90 +/- 2%

With the above chemical composition, the device design (discussed in detail below) is the best mode of performing the invention:

The arrangement of the apparatus is designed in a certain manner so as to achieve maximum killing of the mosquitoes. The apparatus comprises of the following components namely: 1. Heating element, 2. Chemical container, 3. Graphite Rod, 4. Five Nos. of Ultra Violet Light Emitting Diode (UV LED), 5. Fan, 6.Titanium dioxide coated Fan Holder, 7.Mosquito Collecting Tray. The Graphite Rod is used to evenly distribute heat throughout the body of the device in order to maximize its visibility in the Infra Red (IR) vision of the mosquitoes. An Ultra Violet Light Emitting Diode (UV LED) is used for excitement of the Titanium Di Oxide (T1O2) coated area in order to produce CO2 and moisture as a result of photo-catalytic reaction/This combines with the chemical vapors and produces a unique CO2 signature. Hence 5 UV LEDs are used for the desired optimal effect. Also the need for light would attract the mosquitoes towards the device. A heating element is used to vaporize the chemical in a temperature range identical to that of human beings (37 °C). The heat produced by the heating element, CO₂ produced by T1O2 coated area, UV LED and the chemical composition are used to attract the mosquitoes. The coated surface area lies below the LED in order to get the light for the excitement of the area. A fan is placed 7-10 mm beneath the point of tapered end for suction & killing of the mosquitoes by dehydration (asphyxiation). It is also used to suck the mosquitoes that enter through the gap (optimized at 15-18 mm height) in the device. In order to aishieve this effect, parameters such as fan speed and taper angle are considered. The liquid containing chemical composition is placed at the top of the device and the LEDs are placed below the liquid container. The coated surface area lies below the LED in order to get the light for the excitement of the area. A fan is placed beneath the point of tapered end for suction & killing of the mosquitoes by dehydration. A taper angle is maintained between 17 and 25 degree in order to have maximum suction area so that mosquitoes get sucked and then trapped below the fan blade, without any blood spatter. The debris of the killed mosquitoes can be collected using the collecting tray.

Advantages of the invention

• Since the device is a mosquito killer, it reduces mosquito populations in areas of human inhabitation.

• The simultaneous masking and attraction feature is unique, one of a kind and first to be done.

• It ensures that the users have a good night's sleep, free from mosquito bites, thereby protected from harmful diseases like Malaria, Dengue and Chikungunya.

• Further to this, the device also functions as an air purifying system by reducing the microbial load in the environment around it. It brings freshness to the room.

• The dry debris is collected on a mosquito collecting tray, which can be easily removed from the device and dusted off. The collecting tray will be available as disposable units, which can be replaced when ever required.

• The device also has a rechargeable battery (back up for 8 hours), that makes it usable in times of power outages. ^*optional

• The device also has a level indicator to indicate that the chemical level is low and that it needs to be replaced by the refill pack.* optional

• The device is low cost, consumes less power, is compact, light weight and eco-friendly when compared to similar other products. • It is non-toxic and safe for use by households having infants and pregnant women.

• It does not give any offensive smell or other side effects like allergies or headaches

• The design allows the internal frame (fixed) work to be made with any one of the outer casings provided. Hence, this_. adds to its aesthetic value.

Drawings with brief description:

Figure 1 : View of the assembled device, consisting of parts (1)-(7).

The top cap can be easily moved to place the chemical container (available as the refill pack). The mosquito tray is located at the bottom of the device, from where it can be easily detached and the dry debris dusted off.

Figure 2: (1) Heating element

The heating element provides heat that vaporizes the liquid chemical present in the chemical container.

Figure 3: Container cap

It is made of plastic and is used to seal and thereby prevent leakage of the chemical liquid from the chemical container.

Figure 4: (2) Chemical container

It is the permanent container that holds the chemical liquid.

Figure 5: (3) Graphite rod

It is a porous wick that dissipates the chemical into the air, when the device is turned on. Figure 6: (4) Six Ultra Violet Light Emitting Diode (UV LED)

6 nos. of UV LED are used in the device to both produce light and act as a reactant for photo-catalytic reaction optimally.

Figure 7: (5) Fan

The fan is an integral part of the suction mechanism in the device. Figure 8: Motor

The motor powers the fan and is also an integral part of the suction mechanism in the device.

Figure 9: Liquid holder

Liquid holder is the device that holds all its constituent parts together. It acts as a binding unit and a protective casing for the Led and Liquid container parts of the device.

Figure 10: Fan and motor holder

Fan and motor holder is the device that holds all its constituent parts together. It acts as a binding unit and a protective casing for the Fan blade and Motor container parts of the device.

Figure 11 : Circuit and Three pin holder

The circuit powers electric current into the device, which in turn powers both the heating and the suction systems of the device. There is an optional battery mode of the device, which performs the same function in the absence of electric power.

Figure 12: Upper dome Upper dome is made of plastic and can be easily moved when the chemical container has to be replaced, and put back into its place again.

Figure 13: (7) Mosquito Tray

The debris is collected in the mosquito tray, from where is can be easily dusted off as the tray is de- and attachable to the device.

Figure 14: Internal frame work [skeleton] of the device and

Figure 15: Cross section of the device

The following figures 14 and 15 is of the optimized internal skeleton of the device, which will remain further unchanged. The outer casings can be changed as per aesthetic demands and fitted to this internal skeleton, which consists of parts (1) to (7), located from top to bottom of the device.

Experimental results

Our research involved OAI test and Masking test.

Combination table for chemical formulations tested:

Example V. 3 concentrations of 12 different ratios of Diethylenediamine were tested with solvents being ionized water /Liquid Paraffin/Both Ionized water and liquid Paraffin, with varying quantities of HEXADECANOIC ACID and BHT.

Diethylenediamine + 5, 10, 15 HEXADECANOICACID (1 :5) 0.88% +

1% BHT + Ionized water + Liquid

paraffin

Diethylenediamine + 5, 10, 15 HEXADECANOICACID (1 :10) 0.88%

+ 1% BHT + only Ionized water

Diethylenediamine ^* + 5, 10, 15 HEXADECANOICACID (1 :10) 0.88%

+ 1 % BHT + only Liquid paraffin

Diethylenediamine + 5, 10, 15 HEXADECANOIC ACID (1 :10) 0.88%

+ 1% BHT + Ionized water + Liquid

paraffin

Diethylenediamine ^■ + 5, 10, 15 HEXADECANOIC ACID (1 :5) 0.88%

+ 0.8% BHT + only Ionized water

Diethylenediamine + 5, 10, 15 HEXADECANOIC ACID (1 :5) 0.88%

+ 0.8% BHT + only Liquid paraffin

Diethylenediamine + 5, 10, 15 HEXADECANOIC ACID (1 :5) 0.88%

+ 0.8% BHT + Ionized water + Liquid

paraffin

Diethylenediamine + 5, 10, 15 HEXADECANOIC ACID (1 :10) 0.88%

+ 0.8% BHT + only Ionized water

Diethylenediamine + 5, 10, 15 HEXADECANOIC ACID (1 :10) 0.88%

+ 0.8% BHT + only Liquid paraffin

Diethylenediamine + 5, 10, 15 HEXADECANOIC ACID (1 :10) 0.88% + 0.8% BHT + Ionized water + Liquid

paraffin

Example 2: 3 concentrations of 12 different ratios of Diethylenediamine were tested with distilled water as solvent with various emulsifiers.

HEXADECANOIC ACID (1 : 10) +

0.5% Isopropanol + 1% Emulsifier:

67% of alkylaryl sulfonate, 23% of n- butanol (CAS #90 194-26-6), linear

calcium aryl-sulfonate dissolved in n- butanol + distilled water

18 Diethylenediamine + 1% 5, 10, 15

HEXADECANOIC ACID (1 : 10) +

0.5% Isopropanol + 1% Emulsifier:

100% of fatty acid polyethylene glycol

ether esters, CAS #61791-12-6), a

reaction product of caster oil with 20- 40 Mol of Ethylene oxide + distilled

water

19 Diethylenediamine + 1% 5, 10, 15

HEXADECANOIC ACID (1 : 10) +

0.5% Isopropanol + 1% Emulsifier:

100% of aryl polyglycol ether, CAS

#73297-33-3 + distilled water

20 Diethylenediamine + 1% 5, 10, 15

HEXADECANOIC ACID (1 : 10) +

0.5% Isopropanol + 1% Emulsifier:

100% of polyglycol ether, CAS

#68213-23-0) fatty alcohol + distilled

water

Example 3: 3 concentrations of the given chemical composition was tested

Formulation Chemical composition Concentrations number (microgram/ml)

21 Diethylenediamine (from 0.88% w/w 5, 10, 15

to 0.15%w/w),HEXADECANOIC ACID

(from0% w/w to 0.73%w/w),BHT

Example 4: 3 concentrations of the given chemical composition was tested

OAI Test: For our research, we built mosquito-breeding cages:

Using this breeding cage: (Fig. 16)

1. I could tell in which direction the mosquitoes would be able to sense faster and reach the target, either in horizontal or in vertical direction. This method helps to establish if the domestic model (D Mode) has to be placed in ground level or if it should be suspended from a given height. (This is applicable to enterprising model (E Mode) also.

2. I could calculate and compare the Oviposition attraction values of OC* (our composition example 1 , 2,3 & 4) with that of p-cresol, using the following procedure: The oviposition attraction of OC* was compared with p-cresol by testing them at their optimal concentrations required for oviposition attraction i.e., 10 g ml-1. This experiment was conducted by keeping two disposable bowls in a cage, one with OC* and the other with p-cresol as oviposition media, at the opposite corners. The number of egg rafts laid in the oviposition bowls were counted and percentage of egg rafts laid in each bowl was calculated from the total number of egg rafts laid in both OC* and p-cresol.

Sample preparation: For Example 3

The chemical mixture was made by mixing Ionized water for 3 minutes until it became a homogenous mixture. To this. BHT and isopropyl alcohol was added and it was further stirred for 1 minute. Simultaneous to the above process, Diethylenediamine and HEXADECANOIC ACID was centrifuged in varying ratios (from 1 :0 to1 :10) and was added to the main mixture. After complete mixing for about 20 minutes.

The same procedure was repeated for Examples 1 , 2 and 4 Masking test:

After the OAI test, it was possible to select chemicals that would effectively attract mosquitoes and for these chemicals, further testing of their masking effect was done by the following method. Four persons were present in an 18X14 ft room (room temperature: 35.6°C, R_H: 53%, wind speed: 1- 1.2 m/s) and the sample was placed at the center. 10 mosquitoes were released into the room. It was observed if the chemical was able to mask the persons present in the room by observing the mosquito's movement: whether it was able to detect the presence of humans and come towards them or if it was unable to do so.

Evaluation: As 10 mosquitoes were used on the whole, every time a mosquito was able to detect 1 person and bite him, it was taken as a reduction of 10% of masking efficiency of the chemical. In that case, 2 mosquito bites would mean that the chemical is 80% masking efficient and so on. It is illustrated in fig. 17.

Results:

The following table has the OAI values of the control (p-cresol) solution: Concentration Percentage oviposition OAI of

in pg ml-1 in p- cresol ± SE p-cresol

5 59.95 ± 7.18 0.20

10 73.60 ± 3.59 0.47

15 65.99 ± 4.17 0.32

In the case of p-cresol, at 5 pg ml-1 level it was not attractive to gravid females as the OAI was +0.2. With increasing concentration of 10 pg ml-1 and 15 pg ml-1 , the OAI was respectively +0.47 and +0.32.

The following table has the OAI values of our chemical sample vs. the control solution:

Optimum concentration for oviposition attraction - In the case of OC^*, among these concentrations tested, samples having OAI> 0.47 were taken for further optimization testing.

The following table contains the results of the Masking effect test:

Example Formulation Chemical composition % Masking efficiency No. number

1 1 Diethylenediamine + 90%

HEXADECANOIC ACID (1 :5)

0.88% + 1% BHT + only

Ionized water

2 Diethylenediamine + 80%

HEXADECANOIC ACID (1 :5)

0.88% + 1% BHT + only

Liquid paraffin

3 Diethylenediamine + 80%

HEXADECANOIC ACID (1 :5)

0.88% + 1% BHT + Ionized

water + Liquid paraffin

4 Diethylenediamine + 80%

HEXADECANOIC ACID

(1 :10) 0.88% + 1% BHT +

only Ionized water

5 Diethylenediamine + 60%

HEXADECANOIC ACID

(1 :10) 0.88% + 1% BHT +

only Liquid paraffin

6 Diethylenediamine + 70%

HEXADECANOIC ACID

(1 :10) 0.88% + 1% BHT +

Ionized water + Liquid

paraffin

7 Diethylenediamine + 90%

HEXADECANOIC ACID ( :5)

0.88% + 0.8% BHT + only

Ionized water

8 Diethylenediamine + 90%

HEXADECANOIC ACID (1 :5)

0.88% + 0.8% BHT + only

Liquid paraffin

9 Diethylenediamine + 90%

HEXADECANOIC ACID (1:5)

0.88% + 0.8% BHT + Ionized

water + Liquid paraffin

10 Diethylenediamine + 85% HEXADECANOIC ACID

(1 :10) 0.88% + 0.8% BHT + only Ionized water

11 Diethylenediamine + 75%

HEXADECANOIC ACID

(1 :10) 0.88% + 0.8% BHT + only Liquid paraffin

12 Diethylenediamine + 80%

HEXADECANOIC ACID

(1 :10) 0.88% + 0.8% BHT + Ionized water + Liquid paraffin

2 13 Diethylenediamine + 1% 85%

HEXADECANOIC ACID (1 : 5) + 0.5% Isopropanol + 1% Emulsifier: 67% of alkylaryl sulfonate, 23% of n-butanol (CAS #90 194-26-6), linear calcium aryl-sulfonate dissolved in n-butanol + distilled water

14 Diethylenediamine + 1% 90%

HEXADECANOIC ACID (1 : 5) + 0.5% Isopropanol + 1% Emulsifier: 100% of fatty acid polyethylene glycol ether esters, CAS #61791-12-6), a reaction product of caster oil with 20-40 Mol of Ethylene oxide + distilled water

15 Diethylenediamine + 1% 90%

HEXADECANOIC ACID (1 : 5) + 0.5% Isopropanol + 1% Emulsifier: 00% of aryl polyglycol ether, CAS #73297-33-3 + distilled water

16 Diethylenediamine + 1% 75%

HEXADECANOIC ACID (1 : 5) + 0.5% Isopropanol + 1% Emulsifier: 100% of polyglycol ether, CAS #68213-23-0) fatty alcohol + distilled water

17 Diethylenediamine + 1 % 80%

HEXADECANOIC ACID (1 :

10) + 0.5% Isopropanol + 1% Emulsifier: 67% of alkylaryl sulfonate, 23% of n- butanol (CAS #90 194-26-6), linear calcium aryl-sulfonate

From above results example 3and 4 show the best result in all the case, so we tested the Ovipositor response of gravid Culex quinquefasciatus when they were exposed to varying concentrations of Formulation (Example 3 and 4) vs. p-cresol in different cages.

Further to this, I discovered that blocking sensors AgOr and AgOr2 was viable for a threshold range of OAI value 0.32 - 0.40. Above 0.40, these sensors remain active. Hence, this is the reason why the mosquito is unable to sense humans in the vicinity and yet gets drawn towards the device that has higher OAI value [simultaneous attraction and masking phenomenon].

Chemical liquid:

As mentioned earlier, the unique feature of the chemical formulation is that it performs both masking and attraction simultaneously. The chemical formula consists of Diethylenediamine, Piperonyl butoxide (HEXADECANOIC ACID), Butylated Hydroxy Toluene (BHT), Isopropanol & Ionized water. Diethylenediamine & HEXADECANOIC ACID is used for blocking the olfactory sensors of the mosquitoes in the Olfactory Attractive Index (OAI) range of 0.3 to 0.4 which is the OAI range of Human beings. Hence humans are masked from the mosquitoes & the mosquitoes are attracted towards the device which has the OAI range >0.47. In the above chemical formulation, BHT & Isopropanol creates OAI value of above 0.47 which attracts mosquitoes.

List of parts: ί . Heating element

The heating element provides heat that vaporizes the liquid chemical present in the chemical container. It comprises of a plastic outer casing inside which there is a thermistor. At the centre, there is an aluminium cuffling which transfers heat from thermistor to the wick (graphite rod). The leads of the thermistor are connected to the circuit. To dissipate the liquid to the atmosphere, PTC thermistor is used. The reason for using a PTC thermistor is that a temperature range of 45-50°C is required. The liquid boils and gets converted to vapor efficiently at this temperature range.

2. Chemical container

It is the permanent container that holds the chemical liquid. It is made of PET Plastic, which is highly inert in nature. The main function of the container is to hold the chemical within itself and prevent leakage. The volume of the container is compatible to all the three different refill packs. The Graphite rod (3) is placed in the chemical container.

3. Graphite Rod

It is a porous wick which gets heated when the device is switched on, and this enables the liquid chemical to go into the wick by capillary action where it vaporizes and gets dissipated into the room. The wick is covered with silver coating to increase its life span and for higher conductivity induced capillary action.

4. Six nos. of Ultra Violet Light Emitting Diode (UV LED)

UV LED is generally a reactant for photo catalytic reaction and is also a source of light, which is visible in the IR vision of the mosquito. In the device, UV LEDs act as both source of light and through photo-catalytic reaction, they are also an integral part of the air purification system, which is another feature of the device.

The reason for using 6 nos. of UV LED is: Wavelength of the light necessary for photo-excitation is:

1240 (Planck's constant, h) / 3.2 eV (band gap energy) = 388 nm (UV wavelength range). Hence, UV LED is chosen.

8 watts/ sq.m is the power required to excite the atoms from valence to conduction bond. Hence, 6 LEDs are used to excite the present area.

5. Fan

The fan is made of plastic and held by the fan holder. It is powered by the motor. Upon rotation (3000 rpm), the fan forms a suction system, which pulls the attracted mosquitoes into the device and traps them. The fan is placed beneath the point of tapered end for suction & killing of the mosquitoes by dehydration. A taper angle is maintained between 17 and 25 degree in order to have maximum suction area so that mosquitoes get sucked and then trapped below the fan blade, without any blood spatter.

6. Titanium dioxide coated Fan Holder

The fan holder seats the fan and the coated T1O2 reduces microbial load in the air, when its atoms are excited by UV light. This functions as part of the air purification system,

7. Mosquito Collecting Tray

The mosquito tray is made of plastic. The dry debris is collected here and can be easily discarded by detaching the tray, dusting off the debris and fixing the tray back on to the device. The tray also functions as an air-ventilation system that ensures that the air flow is directed downwards and this makes sure that the mosquitoes do not come out of the device once they are sucked into it.

Other optional features: Level Indicator: It is used to indicate the level of the chemical liquid. Two copper strips are inserted into the liquid. The plastic at the bottom acts as a bridge between the two copper strips. When the level of the liquid rises above 5ml, the circuit gets complete, allowing flow of current making the device work. When the level is at 6ml, it will indicate the red LED and if the level goes below 5ml, the device will stop working. This is useful to prevent wastage of electricity when the device might be left on when there is no liquid. It will also alert the consumer that the container has to be replaced with the refill pack.

Rechargeable battery: enables use of the device in case of power failure (8 hr back up). It also gets automatically charged when power is on.

The above examples and embodiments have been presented for purpose of illustration and description but is not intended to be exhaustive or limiting the invention to the form disclosed. The scope of invention is limited by the scope of claims only. Many modifications and variations will be apparent to those ordinary skill in the art. The embodiments described was chosen in order to best explain the principles of the invention, the practical application and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated. The permutations of various embodiments are expressly within the scope of the invention.

Claims

WE CLAIM:

1. A method for reducing the incidence of disease transmission by mosquitoes comprising administering a composition comprising :

a. a composition A having an olfactory attractive index in the range 0.3 to 0.4 and with human masking effect 90 ± 2%, and

b. a composition B having an olfactory attractive index in the range greater than 0.47,

to a target area, wherein composition A masks humans from the mosquitoes, and wherein composition B attracts contact of the mosquitoes with the target area,

2. The method of claim 1 for reducing the incidence of disease transmission by mosquitoes comprising administering a composition comprising :

a. a composition A which is a Hexadecanoic acid and Diethylenediamine with an olfactory attractive index in the range 0.3 to 0.4, and

b. a composition B which is a combination of Butylated Hydroxy Toluene (BHT) and Isopropanol with an olfactory attractive index greater than 0.47,

to a target area, wherein composition A masks humans from the mosquitoes, and wherein composition B attracts contact of the mosquitoes with the target area.

3. The method of claim 1 for reducing the incidence of disease transmission by mosquitoes comprising administering a composition comprising :

a. a composition A which is a Thiomorpholine, 4-methyl and Diethylenediamine with an olfactory attractive index in the range 0.3 to 0.4, and

4. The method of claim 2, wherein the said composition comprises of :

- Hexadecanoic acid is present in an amount approximately 0.08% and 0.15%w/w,

- Diethylenediamine is present in an amount approximately 0.26% and 0.73%w/w,

- Butylated Hydroxy Toluene (BHT) is present in an amount approximately 1%w/w,

- Isopropanol is present in an amount approximately 1%w/w, and

- Liquid Paraffin or Ionized water is present in an amount approximately 96.12%w/w.

5. The method of claim 3, wherein the said composition comprises of

- Hexadecanoic acid is present in an amount approximately 0.08% and 0.15%w/w, - Thiomorpholine, 4-methyl is present in an amount approximately 0.26% and 0.73%w/w,

- Butylated Hydroxy Toluene (BHT) is present in an amount approximately 1%w/w,

- Isopropanol is present in an amount approximately 1%w/w, and

- Liquid Paraffin or Ionized water is present in an amount approximately 95.40%w/w.

6. The method of claim 4 and 5 wherein the composition further comprises of perfume which is present in an amount approximately 1 % w/w.

7. A method for attracting mosquitoes into a target area comprising applying a composition having an olfactory attractive index greater than 0.47 to the target area.

8. The method of claim 7, wherein composition is a combination of Butylated Hydroxy Toluene (BHT) and Isopropanol.

9. The method of claim 1 to 3 further comprising the steps of :

a. vaporizing the said composition in the target area thereby attracting the mosquitoes into the target area, and

b. generating CO2 in the target area thereby killing the said attracted mosquitoes by asphyxiation.

10. The method of claim 9, wherein vaporizing is at 37°C.

11. The method of claim 9 and 10, wherein the temperature is maintained with heating elements.

12. The method of claim , wherein the heating elements are graphite rods.

3. The method of claim 9, wherein CO2 is generated by exciting Ti0₂ with UV LEDs.

14. A device for reducing the incidence of disease transmission by mosquitoes using the composition claimed in claim 1 comprising of :

a. a housing,

b. a vaporizing means disposed within the said housing for vaporizing the said composition, and

c. a asphyxiation means disposed within the said housing for generating CO2.