WO2014190918A1

WO2014190918A1 - Collecting container for produced gas and manufacturing method therefor

Info

Publication number: WO2014190918A1
Application number: PCT/CN2014/078782
Authority: WO
Inventors: 厉刚
Original assignee: Li Gang
Priority date: 2013-05-29
Filing date: 2014-05-29
Publication date: 2014-12-04
Also published as: CN103289890B; CN103289890A

Abstract

Provided are a collecting container for produced gas used in aerogene cultivation and detection, and a manufacturing method therefor. The collecting container for produced gas comprises: a tube body, the inner side of the lower part thereof being provided with at least two transparent faces, constituting a front observation window and a back observation window; an insert, comprising at least a gas collecting section having a groove towards the bottom of the tube body, with both the front and rear ends of the groove being open; wherein the openings on the front and rear ends of the insert groove are respectively sealed to the front observation window and back observation window at the lower part of the tube body, forming a gas collecting chamber opening downwards. The gas producing phenomenon in the gas collecting chamber can be observed through the faces of the collecting container for produced gas.

Description

Gas production collection container and manufacturing method thereof

The present invention relates to the field of microbial culture and detection technology, and more particularly to a gas production collection container for producing gas-producing microorganisms and detecting the same, and a method of manufacturing the same. Background technique

Microbial decomposition of certain substances during the growth process or the use of external energy to generate gas is a common phenomenon in nature, such as the growth process of coliforms, the fermentation process of yeast, the production of biogas, the photosynthesis of algae, etc. Human beings are widely used in production and life, and are closely related to human life. In the research and utilization of microorganisms, collecting and observing the gases produced by microorganisms is a widely used research method.

1 is a schematic view of a prior art gas collection container for gas culture and detection of microorganisms. Referring to Figure 1, the gas collection container includes: a large test tube, and an inverted small test tube housed in the large test tube. When using the gas collection container, firstly, the configured culture medium is placed in a large test tube, and the liquid level of the culture medium is higher than the top of the small test tube; then, the gas-permeable plug is used for sealing; The residual air in the inverted test tube is dropped; during the reaction of the medium, the gas generated is concentrated on the top of the inverted small test tube.

However, in the process of realizing the present invention, the Applicant has found that the above gas collection container has the following technical defects: (1) The small test tube is vertically set in the middle of the large test tube, and if the turbidity of the medium is large, the inverted test tube is produced in the middle. The gas phenomenon is very difficult to observe, and often results in misjudgment; (2) Some gas-producing microbial culture media are not resistant to high temperatures, and the residual air in the small test tubes cannot be discharged by high temperature and high pressure, and cannot be observed and collected by means of inverted test tubes. gas. Summary of the invention

(1) Technical problems to be solved

In view of the above technical problems, the present invention provides a gas generating collection container and a method of manufacturing the same, and in the case where the turbidity of the medium is large, gas production in the gas generating collection container can be observed as well.

(ii) Technical solutions

According to an aspect of the invention, a gas producing collection container is provided. The gas collection container comprises: a tube body, and at least two transparent planes are formed on the inner side of the lower portion to form a front observation window and a rear observation window; The inner insert has at least a gas collecting portion, the gas collecting portion has a groove facing the bottom of the pipe body, and the groove is open at the front and rear ends; wherein the front and rear openings of the inner insert groove are respectively observed with the lower part of the pipe body The window and the rear observation window are sealed to form a plenum that opens downward.

According to another aspect of the present invention, there is also provided a method of producing the above gas generating collection container. The manufacturing method comprises: separately forming a pipe body and an insert; and inserting the opening of the insert into the pipe body so that the openings at the front and rear ends of the insert groove abut the two transparent planes of the lower portion of the pipe body respectively. Sealed to form a plenum that opens downwards.

According to still another aspect of the present invention, a gas producing collection container is provided. The gas collection container comprises: a tube body, the tube body comprising a flat portion and an arc-shaped surface portion. In a lower portion of the tube body, the curved surface of the tube body is concavely formed to form a plane, and the concavely forms a plane and a pair The flat portion of the side tube body forms two transparent planes to form a front observation window and a rear observation window; the inner insert member includes at least a gas collecting portion having a groove facing the bottom of the tube body, the front and rear ends of the groove The openings of the front and rear ends of the inner recess are respectively sealed with the front observation window and the rear observation window of the lower portion of the tubular body to form a plenum opening downward.

According to still another aspect of the present invention, there is also provided a method of producing the above gas generating collection container. The manufacturing method includes: fabricating a first member including an arcuate face portion of the tubular body and an insert member, the planar portion of the tubular body being open at a position; and a planar portion of the tubular body being coupled to the opening of the first member.

According to still another aspect of the present invention, a gas producing collection container is provided. The gas collection container comprises: a tubular body, which is generally in the form of a test tube, the lower portion of which is symmetrically inwardly recessed to form mutually parallel and transparent two planes, forming a front observation window and a rear observation window; and an inner insert comprising at least gas collection The gas collecting portion has a groove facing the bottom of the pipe body, the groove is open at the front and rear ends, and extends in a direction perpendicular to the central axis of the pipe body; wherein the openings of the front and rear ends of the insert groove are respectively connected with the pipe body The front observation window and the rear observation window abut against the seal to form a plenum that opens downward.

According to still another aspect of the present invention, there is also provided a method of producing the above gas generating collection container. The manufacturing method comprises: separately manufacturing two parts symmetrical, wherein each part is a half of a gas collection container that is cut along a plane passing through a central axis thereof and parallel to the front/rear observation window; and two symmetrical sides Partially combined.

(3) Beneficial effects

It can be seen from the above technical solutions that the gas generating collection container of the present invention and the manufacturing method thereof have The following beneficial effects:

(1) Two front and rear plane observation windows are arranged under the pipe body, and the inner surfaces of the two plane observation windows cooperate with the front and rear openings of the inner gas collecting portion of the inner casing to form a gas collecting chamber, even if the detection sample has a large turbidity, It will affect the observation effect of gas production through the plane observation window;

(2) The cross section of the inner part of the inner part of the inner part is a concave structure with a narrow upper and lower width, and the collected gas in the plenum can be discharged by tilting less than 90°;

(3) The section of the inner part of the inner part of the inner part is an isosceles trapezoid, and the angle between the side and the bottom side is 45°, which facilitates the observation of the gas production phenomenon and facilitates the discharge of the collected gas in the gas collection chamber;

(4) The edge of the plane observation window has a protruding protective strip to prevent the surface observation window from being stained due to impact, abrasion, pollution, etc. during transportation or use;

(5) The insert can be directly embedded in the tube body, or it can be embedded in the gas collection container in the laboratory, which is flexible;

(6) The bottom surface of the pipe body is flat, and the ratio of the height and diameter of the pipe body is between 1 and 9, which can ensure that the pipe body can stand upright independently, avoiding the disadvantage that the existing container cannot stand independently;

(7) The gas collection container is rich in variety and various forms, which can meet the needs of many people in the laboratory. In addition, the manufacturing methods are various and can be produced at low cost according to actual conditions. DRAWINGS

1 is a schematic view of a prior art gas collection container for gas-producing microbial culture and detection; FIG. 2A is a front view of a gas production collection container according to an embodiment of the present invention;

2B is an exploded perspective view of a gas collection container according to an embodiment of the present invention;

3A is a front view of a pipe body in a gas collection container according to an embodiment of the present invention;

Figure 3B is a left side view of the tube body in the gas producing collection container of the embodiment of the present invention;

4A and 4B are cross-sectional views of the tube body along the A-A', B-B' planes in the gas generating collection container shown in Fig. 3B, respectively;

5A is a perspective view of an inner insert in a gas collection container according to an embodiment of the present invention;

Figure 5B is a front elevational view of the inner insert of the gas producing collection container of the embodiment of the present invention;

Figure 6 is a partially enlarged perspective view showing the joint position of the pipe body and the insert member in the gas-generating collecting container shown in Figure 2;

Figure 7 is a schematic view showing a gas collecting portion of a rectangular parallelepiped structure in an inner insert of a gas collecting container according to another embodiment of the present invention; 8A, 8B and 8C are respectively cross-sectional views of a gas collecting portion in an inner insert of a gas collecting container according to an embodiment of the present invention;

9A and 9B are schematic views showing the state of use of the gas generating apparatus shown in Fig. 2A. detailed description

The present invention will be described in detail below with reference to the specific embodiments of the invention and the accompanying drawings. It should be noted that in the drawings or the description of the specification, the same reference numerals are used for similar or identical parts. Implementations not shown or described in the figures are known to those of ordinary skill in the art. In addition, although an example of a parameter containing a particular value may be provided herein, it should be understood that the parameter need not be exactly equal to the corresponding value, but rather approximates the corresponding value within acceptable tolerances or design constraints. In addition, the directional terms mentioned in the following embodiments, such as "upper", "lower", "front", "back", "left", "right", etc., are only referring to the directions of the drawings. Therefore, the directional terminology used is for the purpose of illustration and not limitation.

For ease of understanding, the main components involved in the present invention are first numbered, as follows:

11-front observation window; 12- rear observation window;

13- observation window protection strip;

20-inlay;

21-gas gathering section; 22-positioning section;

30-tube cover;

40-gas collection room.

The present invention provides a gas producing collection container and a method of manufacturing the same. Two front and rear plane observation windows are arranged under the pipe body, and the inner surfaces of the two plane observation windows cooperate with the front and rear openings of the inner gas collecting portion of the inner casing to form a gas collecting chamber, and the gas production in the gas collecting chamber can be visually observed through the plane observation window. phenomenon.

In an exemplary embodiment of the invention, a gas producing collection container is provided. 2A is a front elevational view of a gas producing collection container in accordance with an embodiment of the present invention. Figure 2B is an exploded view of the gas producing collection container of the embodiment of the present invention. Referring to Figures 2A and 2B, the gas producing collection container of the present embodiment comprises: a tubular body 10, an insert member 20 and a tube cover 30. The individual components are described in detail below.

Fig. 3A is a front elevational view of a pipe body in a gas producing collection container according to an embodiment of the present invention. Figure 3B is the hair A left side view of the tube in the gas collection container of the embodiment. Referring to FIGS. 3A and 3B, the tubular body 10 has a circular tube structure as a whole, and its lower portion is concavely symmetrical inwardly to form a flat surface. The concave two planes are parallel and are configured as a front observation window 11 and a rear observation window 12. The reaction inside the pipe body 10 can be observed through the front observation window 11 and the rear observation window 12. It should be noted that, as long as the front observation window 11 and the rear observation window 12 are transparent, the inner insert 20 can collectively constitute a plenum for external observation, that is, the tube body is separated from the front observation window 11 and the rear observation window 12. Other locations outside can also be opaque. However, in consideration of the manufacturing cost, the tube body 10 of the present embodiment is integrally formed and transparent as a whole, and it is not necessary to perform special treatment on the materials of the front observation window 11 and the rear observation window 12.

4A and 4B are cross-sectional views of the tube body along the A-A', B-B, respectively, in the gas-generating collecting container shown in Fig. 3B. As shown in Figs. 4A and 4B, the dimension H of the concave portion of the lower portion of the tubular body 10 satisfies: R/2^R-H^3R/4, where R is the inner diameter of the tubular body 10.

Referring to FIG. 3A and FIG. 4B, in the lower part of the pipe body 10, the front observation window 11 and the rear observation window edge have a convex observation window protection strip 13 to prevent impact, abrasion, pollution, etc. during transportation or use. Observe the window stain.

Referring to FIG. 3A and FIG. 3B, the bottom surface of the pipe body 10 is flat, and the ratio of the height to the radius is between 1 and 18, so that the pipe body can be erected independently without other external force support, and the common test tube is avoided. The disadvantage of not being able to stand alone.

In this embodiment, the tubular body 10 is made of a transparent, corrosion-resistant material. The transparent, corrosion-resistant material includes: glass, polystyrene, etc., and the method of manufacturing the tube 10 can be injection molding or blow molding. However, the present invention does not limit the material and manufacturing method of the pipe body 10.

5A and 5B are a perspective view and a front view, respectively, of the insert of the gas generating collection container of Fig. 2. Referring to Figures 5A and 5B, the insert 20 includes a gas collecting portion 21 and a positioning portion 22. The gas collecting portion 21 has a groove toward the bottom of the transparent pipe body, and the groove is open at both front and rear ends and extends perpendicular to the central axis of the pipe body.

Figure 6 is a partially enlarged perspective view showing the joint position of the pipe body and the insert member in the gas-generating collecting container shown in Figure 2; Referring to FIG. 6, the openings at the front and rear ends of the groove are respectively abutted against the front observation window 11 and the rear observation window 12 of the transparent pipe body 10, thereby constituting the plenum 40 which is open downward. The plenum 40 can be used for gas production collection. As can be seen from the above description, the openings at the front and rear ends of the groove are all planes that match the front and rear viewing, and the vertical distance C of the front and rear ends is equal to the distance between the front observation window 11 and the rear observation window 12. Through the plane observation window, the gas production in the gas collection chamber can be visually observed. Even if the turbidity of the test sample is large, it will not affect the observation effect of gas production through the plane observation window. In addition, the front observation window 11 and the rear observation window 12 can also be used in conjunction with instruments such as spectrometers to detect the components of the gas production.

In the present embodiment, the air collecting portion 21 is constituted by a bent sheet-like structure. The longitudinal section of the groove is an isosceles trapezoid, and the longitudinal section of the groove has the same size as the isosceles trapezoid. In the isosceles trapezoid, the angle between the side edge and the bottom edge is 45°, the width A of the trapezoidal bottom edge is smaller than the width of the front/rear observation window, and the height B is smaller than the height of the front/rear observation window. The thickness C of the gas collecting portion 21 is equal to the distance between the front observation window 11 and the rear observation window 12. The depth of the groove is between 1 cm and 2 cm.

Referring to FIG. 6, there is a certain friction between the front and rear ends of the gas collecting portion 21 and the observation window at the corresponding position. By the frictional force, the gas collecting portion can be fixed to the corresponding position of the observation window without being in the medium. Floating under the effect of buoyancy.

In other embodiments of the present invention, the gas gathering portion 21 may also have a body configuration, for example, a rectangular parallelepiped, a cylinder, a dome, or the like extending perpendicular to the central axis of the tubular body. Fig. 7 is a schematic view showing a gas collecting portion of a rectangular parallelepiped structure in an inner insert of a gas producing container according to another embodiment of the present invention. The cylindrical or 集-shaped gas gathering portion is similar to this and will not be described here.

In other embodiments of the present invention, the longitudinal section of the groove in the gas collecting portion 21 may also be one or a combination of the following shapes: for example, a trapezoid (as shown in Fig. 5B), a triangle, a circular arc, or the like. 8A, 8B and 8C are respectively cross-sectional views of the gas collecting portion in the inner insert of the gas collecting container according to the embodiment of the present invention. In Fig. 8A, the groove of the gas collecting portion is triangular; in Fig. 8B, the groove of the gas collecting portion is a combination of a trapezoidal shape and a circular arc shape; in Fig. 8C, the groove of the gas collecting portion is a circular arc shape. In other embodiments of the invention, the shape and size of the recess can be set as desired.

The shape of the longitudinal section of the groove described above is common in that the bottom is narrow and wide. The groove with a narrow opening at the bottom is used, and when the pipe body 10 is inclined, the gas in the plenum 40 can be discharged under the action of buoyancy and surface tension, specifically: (1) after the medium is injected, it can be inclined The way of exhausting the residual air in the plenum 40 is very advantageous for the gas-producing microorganisms which are not resistant to high temperature; (2) After the gas is produced in the medium, the gas generated in the plenum 40 can be collected by tilting without Invert the tube.

In each of the above embodiments, the shape and size of the front and rear end faces of the groove are the same. However, the present invention is not limited thereto. For example, the longitudinal section of the front end surface of the groove has a trapezoidal shape, and the longitudinal section of the rear end surface has a triangular shape; or the cross-sectional shape of the front and rear end surfaces of the groove is a trapezoid of different sizes, which are all the above. Similar variations of the various embodiments are intended to be included within the scope of the invention.

Referring to Fig. 5A and Fig. 5B, in the present embodiment, the positioning portion 22 has a sheet shape having a predetermined thickness and height, and extends symmetrically from the side of the gas collecting portion 21 to the outside to abut against the wall of the side of the front/rear observation window. Due to the predetermined thickness, there is a frictional force between the left and right ends of the positioning portion 22 and the wall of the corresponding position. By virtue of this frictional force, the gas gathering portion 21 is accurately positioned in the middle of the observation window at the bottom of the test tube. Due to the predetermined height, the positioning portion 22 can be symmetrical to the inside of the tubular body without being tilted left and right. The gas collecting portion 21 fixed to the positioning portion is also not inclined left and right. Further, the central axis of the plenum coincides with the central axis of the tubular body.

Referring to FIG. 5A and FIG. 5B, the height of the positioning portion 22 is slightly higher than the air collecting portion, that is, the highest point of the positioning portion 22 is higher than the highest point of the air collecting portion 10, and the lowest point of the positioning portion 22 is lower than the set. The lowest point of the gas portion 20. The advantage of the structure positioning portion is that the highest point of the positioning portion 22 is higher than the highest point of the air collecting portion 10 so that the inverted in-line structure is not easily deformed or bent during the assembly process, and the lowest point of the positioning portion 22 is lower than the set. The lowest point of the gas portion 20 causes a gap between the gas collecting portion and the bottom of the test tube, so that the bubbles in the gas collecting portion can smoothly escape from the gas collecting portion when the test tube is inclined.

Further, the four corners of the positioning portion 22 which are in contact with the pipe wall of the pipe body 10 are notched or rounded to avoid damage to the pipe body by the positioning portion 22.

Of course, the frictional force between the air collecting portion 21 and the front and rear viewing windows is sufficiently large, and the positioning portion 22 can be omitted in the case where the air collecting portion is located in the middle of the front and rear viewing windows of the test tube and the left and right balances are symmetrical.

In this embodiment, the insert 20 is made of an elastic material such as polyethylene or polypropylene. The method of manufacturing the insert 20 can be injection molding.

The cap is used to isolate the medium in the tube from the outside environment. In this embodiment, the tube cover is made of a rubber screw-in cover. However, in the present invention, the tube cover may also be in the form of a snap-fit cover, a plug-in cover or the like, and the material may be plastic, glass or the like in addition to rubber. Those skilled in the art can freely select as needed, and the present invention is not limited.

Moreover, it will be apparent to those skilled in the art that the gas collection device of the present invention may also not include a tube cover, nor does it affect the achievement of the present invention.

The following describes the method of using the gas collection container of the present embodiment: First, the medium is directly filled into the tube body 10, and the gas collection chamber is filled with gas, as shown in Fig. 9A; the tube is turned to the right (or to the left) Tilting about 45°, the air bubbles in the plenum 40 are naturally discharged under buoyancy, and the plenum 40 The inside is filled with liquid, as shown in FIG. 9A; thereafter, the culture medium is cultured, and the gas collection reaction of the gas-producing microorganisms in the gas collection chamber 40 can be visually observed through the observation window, such as the gas production process, and the gas collection chamber 40 is filled with air bubbles. And if it is desired to discharge the generated gas, the tube is also tilted to the right (or to the left) by about 45°, and the bubbles in the plenum 40 are naturally discharged by buoyancy.

It can be seen from the above-mentioned method of use that, with the gas collection container of the present embodiment, the medium which is not resistant to high temperature can be filled in a gas collection container by a sterile cold filling process, and the gas collection is discharged by tilting the test tube by 45°. Air bubbles in the room. In addition, the gas collection container of the present embodiment can be used for industrial production of pre-filled medium reagents, thereby greatly reducing the labor intensity in the laboratory and liberating the labor productivity of the inspectors.

It should be clear to those skilled in the art that the gas collection container of the present embodiment needs to insert the insert 20 into the tube body 10 during use, and the tube body 10 and the insert member 20 can be transported and sold. Separation. That is to say, the insert 20 can be placed into the tube 10 during the production process, or both can be sold and transported separately, and during use, the insert 20 is pushed by a sterile rod-like object. Pressed to the position of the tube observation window, and the two parallel faces of the observation window are combined into a closed structure plenum.

Two methods of manufacturing the above-described gas collection container are described below.

In the first manufacturing method, the tube body and the insert member are separately fabricated; then the opening of the insert member is placed downward, and placed in the tube body, so that the openings at the front and rear ends of the recess of the insert member are respectively opposite to the lower portion of the tube body. The transparent plane abuts the seal and constitutes a plenum that opens downward, thereby producing a gas collection container.

In the second manufacturing method, firstly, two portions which are bilaterally symmetrical, wherein each portion is half of the gas collecting container shown in Fig. 2A, which is cut along a plane passing through its central axis and parallel to the front/rear viewing window And then combine the two parts of the left and right symmetry.

The tube body and the insert member are made of the same material and are one of the following materials: glass, polystyrene, polyethylene or polypropylene, and the two sides of the left and right symmetry are joined together by means of splicing.

It should be noted that with the development of the splicing technology, two materials of different materials can also be combined by welding. Therefore, if this manufacturing method is adopted, the two parts of the left and right symmetry can adopt different materials. To manufacture.

In a second exemplary embodiment of the invention, an additional gas collection container is also provided. The gas collection container differs from the gas collection container of the first embodiment described above in that: the tube body is a square test tube having the same thickness and the same shape, and the lower two transparent surfaces are combined with the inner insert to form the front portion. Rear viewing window and downward plenum.

The manufacturing method of the gas generating container of this embodiment is the same as that of the first embodiment described above, and will not be repeated here.

In a third exemplary embodiment of the invention, an additional gas collection container is also provided. The upper part of the gas collecting container has a circular tubular structure, and the lower part of the tube body has a square tubular structure, and the two transparent surfaces of the square tubular structure at the lower part of the tube body are combined with the inner insert to form a front and rear observation window and a downward plenum. .

In a fourth exemplary embodiment of the invention, an additional gas collection container is also provided. The body of the tube of the gas collection container includes a flat portion and an arcuate surface. In the lower portion of the tubular body, the curved face portion of the tubular body is recessed inwardly to form a plane, and the plane formed by the recess forms a transparent plane with the flat portion of the opposite tubular body. The two transparent faces are combined with the insert to form a front and rear viewing window and a downward plenum.

The gas generating container of this embodiment can be produced by the manufacturing method of the first embodiment described above. In addition, another specific manufacturing method is provided: a first component is formed, the first component includes an arcuate face portion and an inner insert of the tubular body, and a planar portion of the tubular body is opened; the flat portion of the tubular body is coupled to The opening of the first component.

Wherein, the curved surface of the pipe body of the pipe body and the insert are integrally formed by injection molding. The plane of the tube is a plastic film. The plastic film is bonded to the opening of the first component by heat sealing.

In addition, the plane of the tube body can also be a plastic sheet. The manner of combining with the first component is the same as that of the above embodiment, and will not be repeated here.

Heretofore, four embodiments of the present invention have been described in detail with reference to the accompanying drawings. Based on the above description, those skilled in the art should have a clear understanding of the gas producing container of the present invention.

Furthermore, the above definitions of the various elements are not limited to the various specific structures or shapes mentioned in the embodiments, and those skilled in the art can simply and well replace them, for example:

(1) The test tube can also be changed to a cube, a cuboid or a bottle;

(2) The external gas extraction structure can be set on the gas collection chamber to facilitate gas sampling analysis. For example, a rubber penetrating plug is installed in the center of the bottom of the groove (top of the gas collecting chamber), and the gas collecting chamber 40 is sampled by the needle punching method; (3) In addition to the front observation window and the rear observation window, other portions of the tubular body may be transparent or opaque.

In summary, the present invention provides a gas producing collection container. The lower part of the gas collection container is recessed inwardly to form two front and rear observation windows, and the front and rear observation windows and the gas collection part of the inner part constitute a gas collection chamber, and the experimenter can intuitively observe the gas production condition of the medium, The earth is convenient for the use of experimental personnel.

The specific embodiments of the present invention have been described in detail with reference to the preferred embodiments of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and scope of the present invention are intended to be included within the scope of the present invention.

Claims

Rights request

1. A gas collection container, characterized in that it includes:

The pipe body has at least two transparent planes formed on the inner side of the lower part to form the front observation window and the rear observation window; the embedded part at least includes a gas collection part, the gas collection part has a groove toward the bottom of the pipe body, and the front and rear ends of the groove to open one's mouth;

Among them, the openings at the front and rear ends of the groove of the embedded part are sealed with the front observation window and the rear observation window at the lower part of the tube body respectively, forming a downwardly opening air collecting chamber.

2. The gas production collection container according to claim 1, characterized in that the lower part of the tube body is a square container, and the front and back of the square container form the two transparent planes.

3. The gas collection container according to claim 1, characterized in that:

The tube body of the tube body includes a flat surface and an arcuate surface;

In the lower part of the tube body, the arc-shaped surface of the tube body is concave inward to form a plane, and the plane formed by the concave surface and the flat part of the opposite side of the tube body form the two transparent planes.

4. The gas collection container according to claim 1, characterized in that:

The entire tube body has a test tube structure, and its lower part is symmetrically concave forward and backward to form the two transparent planes parallel to each other; and

The groove of the gas collection part of the embedded part extends in a direction perpendicular to the central axis of the pipe body;

Wherein, the openings at the front and rear ends of the insert groove are in contact and sealed with the front observation window and the rear observation window of the tube body respectively, forming a downwardly opening air collection chamber.

5. The gas production collection container according to claim 4, characterized in that the inwardly concave dimension H of the lower part of the tube body satisfies:

R/2^R-H^3R/4

Where, R is the inner diameter of the upper part of the pipe body.

6. The gas production collection container according to claim 1, characterized in that, in the gas collection part of the built-in part, the longitudinal section of the groove has a shape with a narrow bottom and a wide opening.

7. The gas collection container according to claim 6, characterized in that the depth of the groove is between 1 cm and 2 cm.

8. The gas collection container according to claim 6, characterized in that the longitudinal section of the groove is one or a combination of two of the following shapes: trapezoid, triangle or arc shape.

9. The gas collection container according to claim 8, characterized in that: the groove The longitudinal section is an isosceles trapezoid, and the angle between the base and the waist of the trapezoid is 45°.

10. The gas collection container according to claim 1, characterized in that the gas collection part of the embedded part has a sheet-like structure or a body-like structure.

11. The gas production collection container according to claim 1, characterized in that the insert further includes:

The positioning part is in the shape of a sheet with a preset thickness and height, and extends symmetrically from the side of the gas collecting part to the outside, and resists the pipe walls on both sides of the two transparent planes.

12. The gas production collection container according to claim 11, characterized in that the highest point of the positioning part is higher than the highest point of the gas collecting part, and the lowest point is lower than the lowest point of the gas collecting part.

13. The gas production collection container according to claim 11, characterized in that the four corners of the positioning portion in contact with the pipe body wall are notch-shaped or rounded arc-shaped.

14. The gas collection container according to claim 11, characterized in that the gas collection part and the positioning part are integrally formed, and the material thereof is an elastic material.

15. The gas production collection container according to claim 1, further comprising: a gas extraction mechanism, disposed at the center of the bottom of the groove of the gas collection part of the built-in component, for collecting gas generated in the gas collection chamber. Take samples.

16. The gas collection container according to claim 15, characterized in that the gas taking mechanism is a rubber puncture plug.

17. The gas production collection container according to claim 1, characterized in that the edges of the front observation window and the rear observation window have protruding observation window protection strips.

18. The gas collection container according to claim 1, characterized in that the bottom surface of the tube body is flat.

19. The gas production collection container according to any one of claims 4 to 18, characterized in that the entire tube body has a cylindrical, cube, rectangular or bottle type test tube structure.

20. The gas collection container according to any one of claims 1 to 18, further comprising:

The tube cover is fastened to the upper opening of the tube body to isolate the culture medium in the tube body from the external environment.

21. The gas production collection container according to any one of claims 1 to 18, characterized in that the material of the tube body is glass or polystyrene, and its manufacturing method is injection molding or blow molding. type.

22. The gas collection container according to any one of claims 1 to 18, characterized in that the material of the insert is polyethylene or polypropylene, and the manufacturing method is injection molding.

23. A method for manufacturing a gas production collection container according to any one of claims 1 to 22, characterized in that it includes:

Producing the tube body and the insert separately; and

Put the opening of the embedded part downward into the pipe body, so that the openings at the front and rear ends of the groove of the embedded part are in contact and sealed with the two transparent planes at the lower part of the pipe body, forming a downward opening. Gas chamber.

24. A method for manufacturing the gas production collection container described in claim 3, characterized in that it includes:

Producing a first component, which includes an arc-shaped surface portion of the pipe body and the insert, with an opening at the flat portion of the pipe body;

Combine the flat portion of the tube body with the opening of the first component.

25. The manufacturing method according to claim 24, characterized in that the flat portion of the tube body is a plastic film or plastic sheet;

The plastic film or plastic sheet is combined with the opening of the first component through heat sealing.

26. The manufacturing method according to claim 24, characterized in that the arc-shaped surface of the tube body and the insert are integrally formed by injection molding.

27. A method for manufacturing the gas production collection container described in claim 4, characterized in that it includes:

Make two symmetrical parts, respectively, where each part is half of the gas collection container cut along a plane passing through its central axis and parallel to the front/rear observation window; and

Join the two symmetrical parts together.

28. The manufacturing method according to claim 27, characterized in that the two symmetrical parts are joined together by welding;

The tube body and the embedded part are made of the same material, which is one of the following materials: glass, polystyrene, polyethylene or polypropylene.