WO2014183744A3 - Anhydrous immobilization of enzymes - Google Patents

Anhydrous immobilization of enzymes Download PDF

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Publication number
WO2014183744A3
WO2014183744A3 PCT/DE2014/100152 DE2014100152W WO2014183744A3 WO 2014183744 A3 WO2014183744 A3 WO 2014183744A3 DE 2014100152 W DE2014100152 W DE 2014100152W WO 2014183744 A3 WO2014183744 A3 WO 2014183744A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
enzymes
immobilization
anhydrous
polymerization
Prior art date
Application number
PCT/DE2014/100152
Other languages
German (de)
French (fr)
Other versions
WO2014183744A2 (en
Inventor
Susann BARIG
Klaus-Peter Stahmann
Klaus Schnitzlein
Sandra Schröter
Original Assignee
Brandenburgische Technische Universität Cottbus-Senftenberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandenburgische Technische Universität Cottbus-Senftenberg filed Critical Brandenburgische Technische Universität Cottbus-Senftenberg
Publication of WO2014183744A2 publication Critical patent/WO2014183744A2/en
Publication of WO2014183744A3 publication Critical patent/WO2014183744A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/096—Polyesters; Polyamides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds

Abstract

The invention relates to a method for universal immobilization of enzymes on solid surfaces, wherein an enzyme is provided and the enzyme is converted into a dried form or suspended in an organic solvent. The enzyme is mixed with resins prior to polymerization, the polymerization is initiated and thus an immobilized enzyme/enzyme-resin copolymer is obtained, wherein the enzymes are mixed with the resin anhydrously.
PCT/DE2014/100152 2013-05-13 2014-05-02 Anhydrous immobilization of enzymes WO2014183744A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102013104906.0 2013-05-13
DE102013104906.0A DE102013104906B4 (en) 2013-05-13 2013-05-13 Anhydrous immobilization of enzymes

Publications (2)

Publication Number Publication Date
WO2014183744A2 WO2014183744A2 (en) 2014-11-20
WO2014183744A3 true WO2014183744A3 (en) 2015-01-15

Family

ID=51302611

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2014/100152 WO2014183744A2 (en) 2013-05-13 2014-05-02 Anhydrous immobilization of enzymes

Country Status (2)

Country Link
DE (1) DE102013104906B4 (en)
WO (1) WO2014183744A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1557944A (en) * 1978-01-30 1979-12-19 Sumitomo Chemical Co Enzyme immobilization carrier and preparation thereof
EP0579928A1 (en) * 1992-05-25 1994-01-26 The Nisshin Oil Mills, Ltd. Immobilized lipase, process for producing the same and process for transesterifying oil and fat with the same
JP2009275233A (en) * 2002-05-16 2009-11-26 F Hoffmann-La Roche Ag Manufacturing method of polymer layer

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57118792A (en) * 1981-01-19 1982-07-23 Ajinomoto Co Inc Preparation of immobilized enzyme used in non-aqueous reaction

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1557944A (en) * 1978-01-30 1979-12-19 Sumitomo Chemical Co Enzyme immobilization carrier and preparation thereof
EP0579928A1 (en) * 1992-05-25 1994-01-26 The Nisshin Oil Mills, Ltd. Immobilized lipase, process for producing the same and process for transesterifying oil and fat with the same
JP2009275233A (en) * 2002-05-16 2009-11-26 F Hoffmann-La Roche Ag Manufacturing method of polymer layer

Also Published As

Publication number Publication date
WO2014183744A2 (en) 2014-11-20
DE102013104906A1 (en) 2014-11-27
DE102013104906B4 (en) 2015-06-25

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