WO2014170866A3 - Process of obtaining glycoprotein composition with increased galactosylation content - Google Patents

Process of obtaining glycoprotein composition with increased galactosylation content Download PDF

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Publication number
WO2014170866A3
WO2014170866A3 PCT/IB2014/060812 IB2014060812W WO2014170866A3 WO 2014170866 A3 WO2014170866 A3 WO 2014170866A3 IB 2014060812 W IB2014060812 W IB 2014060812W WO 2014170866 A3 WO2014170866 A3 WO 2014170866A3
Authority
WO
WIPO (PCT)
Prior art keywords
glycoprotein composition
increased galactosylation
galactosylation content
content
obtaining
Prior art date
Application number
PCT/IB2014/060812
Other languages
French (fr)
Other versions
WO2014170866A2 (en
Inventor
Guruvasuthevan Ramasamy THUDUPPATHY
Mohit NARESH
Rekha HARIHARAN
Sama NARESH BABU
Indraneel DASARI
Original Assignee
Dr. Reddy's Laboratories Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr. Reddy's Laboratories Limited filed Critical Dr. Reddy's Laboratories Limited
Publication of WO2014170866A2 publication Critical patent/WO2014170866A2/en
Publication of WO2014170866A3 publication Critical patent/WO2014170866A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A cell culture process for production of glycoprotein composition with increased galactosylation content while maintaining specific productivity is provided. The process involves culturing cells at high pCO 2 and subjecting cells to temperature downshift to obtain a glycoprotein with specific glycoprotein composition.
PCT/IB2014/060812 2013-04-18 2014-04-17 Process of obtaining glycoprotein composition with increased galactosylation content WO2014170866A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1729/CHE/2013 2013-04-18
IN1729CH2013 2013-04-18

Publications (2)

Publication Number Publication Date
WO2014170866A2 WO2014170866A2 (en) 2014-10-23
WO2014170866A3 true WO2014170866A3 (en) 2015-04-16

Family

ID=51731926

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2014/060812 WO2014170866A2 (en) 2013-04-18 2014-04-17 Process of obtaining glycoprotein composition with increased galactosylation content

Country Status (1)

Country Link
WO (1) WO2014170866A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU231463B1 (en) 2015-08-04 2024-01-28 Richter Gedeon Nyrt. Method for increasing the galactose content of recombinant proteins
EP3833688A1 (en) * 2018-08-10 2021-06-16 Genentech, Inc. Cell culture strategies for modulating protein glycosylation
WO2023021532A1 (en) * 2021-08-20 2023-02-23 Dr. Reddy’S Laboratories Limited A process to produce a pharmaceutical composition

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999022764A1 (en) * 1997-10-31 1999-05-14 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
WO2001059089A2 (en) * 2000-02-08 2001-08-16 Genentech, Inc. Improved galactosylation of recombinant glycoproteins
US20040136986A1 (en) * 1997-10-31 2004-07-15 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
CN1820080A (en) * 2002-12-23 2006-08-16 布里斯托尔-迈尔斯斯奎布公司 Product quality enhancement in mammalian cell culture processes for protein production
CN101541950A (en) * 2006-07-13 2009-09-23 惠氏公司 Production of glycoproteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999022764A1 (en) * 1997-10-31 1999-05-14 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
US20040136986A1 (en) * 1997-10-31 2004-07-15 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
WO2001059089A2 (en) * 2000-02-08 2001-08-16 Genentech, Inc. Improved galactosylation of recombinant glycoproteins
CN1820080A (en) * 2002-12-23 2006-08-16 布里斯托尔-迈尔斯斯奎布公司 Product quality enhancement in mammalian cell culture processes for protein production
CN101541950A (en) * 2006-07-13 2009-09-23 惠氏公司 Production of glycoproteins

Also Published As

Publication number Publication date
WO2014170866A2 (en) 2014-10-23

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