WO2014164874A3 - Methods and compositions for modulation of efficiency of nucleic acid amplification reactions - Google Patents

Methods and compositions for modulation of efficiency of nucleic acid amplification reactions Download PDF

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Publication number
WO2014164874A3
WO2014164874A3 PCT/US2014/023680 US2014023680W WO2014164874A3 WO 2014164874 A3 WO2014164874 A3 WO 2014164874A3 US 2014023680 W US2014023680 W US 2014023680W WO 2014164874 A3 WO2014164874 A3 WO 2014164874A3
Authority
WO
WIPO (PCT)
Prior art keywords
modulation
methods
efficiency
compositions
nucleic acid
Prior art date
Application number
PCT/US2014/023680
Other languages
French (fr)
Other versions
WO2014164874A4 (en
WO2014164874A2 (en
Inventor
Tobin Hellyer
James Nadeau
Original Assignee
Becton, Dickinson And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2013/067604 external-priority patent/WO2014070946A1/en
Application filed by Becton, Dickinson And Company filed Critical Becton, Dickinson And Company
Publication of WO2014164874A2 publication Critical patent/WO2014164874A2/en
Publication of WO2014164874A3 publication Critical patent/WO2014164874A3/en
Publication of WO2014164874A4 publication Critical patent/WO2014164874A4/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

Provided herein are methods and kits for modulating the amplification efficiency of nucleic acids, which are useful in multiplex reactions where the amplification efficiency of one or more nucleic acids in the mixture are desired to be modulated relative to one or more other nucleic acids. Embodiments relate to molecular diagnostics, including detecting sequence variants, such as SNPs, insertions deletions, and altered methylation patterns, as well as the modulation of the amplification efficiency of internal control sequences to provide more accurate control sequences for amplification reactions.
PCT/US2014/023680 2013-03-13 2014-03-11 Methods and compositions for modulation of amplification efficiency WO2014164874A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361779416P 2013-03-13 2013-03-13
US61/779,416 2013-03-13
USPCT/US2013/067604 2013-10-30
PCT/US2013/067604 WO2014070946A1 (en) 2012-10-31 2013-10-30 Selective amplification and real-time pcr detection of rare mutations

Publications (3)

Publication Number Publication Date
WO2014164874A2 WO2014164874A2 (en) 2014-10-09
WO2014164874A3 true WO2014164874A3 (en) 2015-01-08
WO2014164874A4 WO2014164874A4 (en) 2015-03-12

Family

ID=51659307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/023680 WO2014164874A2 (en) 2013-03-13 2014-03-11 Methods and compositions for modulation of amplification efficiency

Country Status (1)

Country Link
WO (1) WO2014164874A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109601008B (en) * 2016-04-01 2023-02-28 克罗玛科德公司 Competitive probes for engineered signal generation
WO2020092134A1 (en) * 2018-10-29 2020-05-07 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
DE102022003724B3 (en) 2022-10-10 2024-02-29 "REME-Möbelbeschläge GmbH" Ejection device for a movable furniture part

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO2010111682A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
WO2012151560A2 (en) * 2011-05-04 2012-11-08 Biocept, Inc. Methods for detecting nucleic acid sequence variants
WO2014070946A1 (en) * 2012-10-31 2014-05-08 Becton, Dickinson And Company Selective amplification and real-time pcr detection of rare mutations

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US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
JPH04507402A (en) 1989-05-18 1992-12-24 マイクロプローブ・コーポレーション cross-linked oligonucleotide
US5419966A (en) 1991-06-10 1995-05-30 Microprobe Corporation Solid support for synthesis of 3'-tailed oligonucleotides
US5512677A (en) 1991-08-13 1996-04-30 National Science Council 3-substituted methyl-2,3-dihydroimidazo 1,2-c!quinazoline derivatives, the preparation and use thereof
US5492610A (en) 1994-10-18 1996-02-20 The United States Of America As Represented By The Secretary Of The Army Solid state electrochemical cell for performing electro-chemical measurements on a solid electrolyte at high temperatures
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US5660989A (en) 1995-06-07 1997-08-26 Merck & Co., Inc. DNA polymerase extension assay for influenza virus endonuclease
US5736626A (en) 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
ES2180345T3 (en) 1998-10-07 2003-02-01 Nestle Sa NEW PROCEDURE FOR THE CREW-CONSERVATION OF PLANTS.
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
WO2003020739A2 (en) 2001-09-04 2003-03-13 Exiqon A/S Novel lna compositions and uses thereof
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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO2010111682A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
WO2012151560A2 (en) * 2011-05-04 2012-11-08 Biocept, Inc. Methods for detecting nucleic acid sequence variants
WO2014070946A1 (en) * 2012-10-31 2014-05-08 Becton, Dickinson And Company Selective amplification and real-time pcr detection of rare mutations

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOHN MORLAN ET AL: "Mutation Detection by Real-Time PCR: A Simple, Robust and Highly Selective Method", PLOS ONE, PUBLIC LIBRARY OF SCIENCE, US, vol. 4, no. 2, 25 February 2009 (2009-02-25), pages e4584 - 1, XP008155203, ISSN: 1932-6203 *
LIVAK K J: "Allelic discrimination using fluorogenic probes and the 5' nuclease assay", GENETIC ANALYSIS: BIOMOLECULAR ENGINEERING, ELSEVIER SCIENCE PUBLISHING, US, vol. 14, no. 5-6, 1 February 1999 (1999-02-01), pages 143 - 149, XP004158696, ISSN: 1050-3862, DOI: 10.1016/S1050-3862(98)00019-9 *

Also Published As

Publication number Publication date
WO2014164874A4 (en) 2015-03-12
WO2014164874A2 (en) 2014-10-09

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