WO2014155365A3 - Purification method - Google Patents

Purification method Download PDF

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Publication number
WO2014155365A3
WO2014155365A3 PCT/IB2014/060287 IB2014060287W WO2014155365A3 WO 2014155365 A3 WO2014155365 A3 WO 2014155365A3 IB 2014060287 W IB2014060287 W IB 2014060287W WO 2014155365 A3 WO2014155365 A3 WO 2014155365A3
Authority
WO
WIPO (PCT)
Prior art keywords
purification method
purification
pegylated gcsf
gcsf
molecular weight
Prior art date
Application number
PCT/IB2014/060287
Other languages
French (fr)
Other versions
WO2014155365A2 (en
Inventor
Vivek ARTHANARI
Gopinath GOVINDARAJAN
Jaby Jacob
Bharata Ratnam PARAYITAM
Original Assignee
Dr.Reddy's Laboratories Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr.Reddy's Laboratories Limited filed Critical Dr.Reddy's Laboratories Limited
Publication of WO2014155365A2 publication Critical patent/WO2014155365A2/en
Publication of WO2014155365A3 publication Critical patent/WO2014155365A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

A method for purification of pegylated GCSF is provided. In particular, the application employs a cation exchange chromatography with varying conductivity of wash buffers to separate low and high molecular weight impurities from the desired pegylated GCSF.
PCT/IB2014/060287 2013-03-29 2014-03-29 Purification method WO2014155365A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1430CH2013 2013-03-29
IN1430/CHE/2013 2013-03-29

Publications (2)

Publication Number Publication Date
WO2014155365A2 WO2014155365A2 (en) 2014-10-02
WO2014155365A3 true WO2014155365A3 (en) 2015-02-19

Family

ID=51625563

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2014/060287 WO2014155365A2 (en) 2013-03-29 2014-03-29 Purification method

Country Status (1)

Country Link
WO (1) WO2014155365A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1663962A (en) * 2004-03-01 2005-09-07 重庆富进生物医药有限公司 Recombinant human granulocyte colony stimulating factor and one-step purifying process for chemical modifications thereof
CN1814779A (en) * 2005-12-20 2006-08-09 山东泉港药业有限公司 Method for producing recombinant human granulocyte colony stimulating factor
CN102234310A (en) * 2010-04-30 2011-11-09 杭州九源基因工程有限公司 Polyethylene glycol modified protein separating and purifying method
WO2012057529A2 (en) * 2010-10-29 2012-05-03 Hanmi Holdings Co., Ltd. Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli
CN102485742A (en) * 2010-12-02 2012-06-06 山东新时代药业有限公司 Preparation method and separation and purification method of polyethylene glycol single modified recombinant human granulocyte-colony stimulating factor
CN102892780A (en) * 2010-03-17 2013-01-23 百奥杰诺瑞克斯有限公司 Method for obtaining biologically active recombinant human g-csf

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1663962A (en) * 2004-03-01 2005-09-07 重庆富进生物医药有限公司 Recombinant human granulocyte colony stimulating factor and one-step purifying process for chemical modifications thereof
CN1814779A (en) * 2005-12-20 2006-08-09 山东泉港药业有限公司 Method for producing recombinant human granulocyte colony stimulating factor
CN102892780A (en) * 2010-03-17 2013-01-23 百奥杰诺瑞克斯有限公司 Method for obtaining biologically active recombinant human g-csf
CN102234310A (en) * 2010-04-30 2011-11-09 杭州九源基因工程有限公司 Polyethylene glycol modified protein separating and purifying method
WO2012057529A2 (en) * 2010-10-29 2012-05-03 Hanmi Holdings Co., Ltd. Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli
CN102485742A (en) * 2010-12-02 2012-06-06 山东新时代药业有限公司 Preparation method and separation and purification method of polyethylene glycol single modified recombinant human granulocyte-colony stimulating factor

Also Published As

Publication number Publication date
WO2014155365A2 (en) 2014-10-02

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