WO2014151142A1 - Modulators of methyl modifying enzymes, compositions and uses thereof - Google Patents
Modulators of methyl modifying enzymes, compositions and uses thereof Download PDFInfo
- Publication number
- WO2014151142A1 WO2014151142A1 PCT/US2014/025081 US2014025081W WO2014151142A1 WO 2014151142 A1 WO2014151142 A1 WO 2014151142A1 US 2014025081 W US2014025081 W US 2014025081W WO 2014151142 A1 WO2014151142 A1 WO 2014151142A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ethyl
- alkylene
- methyl
- compound
- piperidin
- Prior art date
Links
- 0 *C(C(*)*1*2C***1)C2C(NCC1=C(*)C(*)=C(*)NC1=O)=O Chemical compound *C(C(*)*1*2C***1)C2C(NCC1=C(*)C(*)=C(*)NC1=O)=O 0.000 description 2
- XDSFRSNYROGWJH-UHFFFAOYSA-N CC(C1CC1)(c1c(C)c(C(OC)=O)c2[n]1nccc2)O Chemical compound CC(C1CC1)(c1c(C)c(C(OC)=O)c2[n]1nccc2)O XDSFRSNYROGWJH-UHFFFAOYSA-N 0.000 description 1
- DOUUXHOBKBVFNB-UHFFFAOYSA-N CC(C1CCN(CCC(F)(F)F)CC1)c1c(C)c(C(O)=O)c2[n]1nccc2 Chemical compound CC(C1CCN(CCC(F)(F)F)CC1)c1c(C)c(C(O)=O)c2[n]1nccc2 DOUUXHOBKBVFNB-UHFFFAOYSA-N 0.000 description 1
- LXJFIPMRAWETMF-UHFFFAOYSA-N CCOC(C)NNC(C(O)=O)=C(C)C Chemical compound CCOC(C)NNC(C(O)=O)=C(C)C LXJFIPMRAWETMF-UHFFFAOYSA-N 0.000 description 1
- HJDCTMJZCZBFRQ-UHFFFAOYSA-N CCOC(c(c(C)c1C(C)C(CC2)CCN2C(C(C)(C)F)=O)c2[n]1nccc2)=O Chemical compound CCOC(c(c(C)c1C(C)C(CC2)CCN2C(C(C)(C)F)=O)c2[n]1nccc2)=O HJDCTMJZCZBFRQ-UHFFFAOYSA-N 0.000 description 1
- XYFVUVBRUBUXGQ-UHFFFAOYSA-N CCOC(c(c(C)c1C(C)C2CCN(CC(C)(C)F)CC2)c2[n]1nccc2)=O Chemical compound CCOC(c(c(C)c1C(C)C2CCN(CC(C)(C)F)CC2)c2[n]1nccc2)=O XYFVUVBRUBUXGQ-UHFFFAOYSA-N 0.000 description 1
- AQILGSICONUFTA-UHFFFAOYSA-N CCOC(c(c(C)c1C(C)C2CCN(CC(C)=O)CC2)c(cc2)[n]1nc2OC)=O Chemical compound CCOC(c(c(C)c1C(C)C2CCN(CC(C)=O)CC2)c(cc2)[n]1nc2OC)=O AQILGSICONUFTA-UHFFFAOYSA-N 0.000 description 1
- VLQIENRORHLVAV-UHFFFAOYSA-N CCOC(c(c(C)c1C(C)C2CCN(CCC(F)(F)F)CC2)c2[n]1nccc2)=O Chemical compound CCOC(c(c(C)c1C(C)C2CCN(CCC(F)(F)F)CC2)c2[n]1nccc2)=O VLQIENRORHLVAV-UHFFFAOYSA-N 0.000 description 1
- POBOWNUDRSLQEJ-UHFFFAOYSA-N CCOC(c(c(C)c1C(C)C2CCNCC2)c(cc2)[n]1nc2OC)=O Chemical compound CCOC(c(c(C)c1C(C)C2CCNCC2)c(cc2)[n]1nc2OC)=O POBOWNUDRSLQEJ-UHFFFAOYSA-N 0.000 description 1
- SNZVQHWQVBAASP-UHFFFAOYSA-N CCOC(c(c(C)c1C(OCC(C)C)=O)c2[n]1nccc2)=O Chemical compound CCOC(c(c(C)c1C(OCC(C)C)=O)c2[n]1nccc2)=O SNZVQHWQVBAASP-UHFFFAOYSA-N 0.000 description 1
- ODLLPSJDANNVTP-UHFFFAOYSA-N CCOC(c(cc1C(C(CC2)CCN2OC(c2ccccc2)=O)=O)c2[n]1nccc2)=O Chemical compound CCOC(c(cc1C(C(CC2)CCN2OC(c2ccccc2)=O)=O)c2[n]1nccc2)=O ODLLPSJDANNVTP-UHFFFAOYSA-N 0.000 description 1
- CECCPWAWRYFLOA-UHFFFAOYSA-N C[n]1nccc1C(OC)=O Chemical compound C[n]1nccc1C(OC)=O CECCPWAWRYFLOA-UHFFFAOYSA-N 0.000 description 1
- WQFOGLYQVFBDEY-UHFFFAOYSA-N C[n]1nccc1CO Chemical compound C[n]1nccc1CO WQFOGLYQVFBDEY-UHFFFAOYSA-N 0.000 description 1
- HNXZQLUJQXZRAG-UHFFFAOYSA-N Cc1c(C(C2CC2)=O)[n]2ncccc2c1C(OC)=O Chemical compound Cc1c(C(C2CC2)=O)[n]2ncccc2c1C(OC)=O HNXZQLUJQXZRAG-UHFFFAOYSA-N 0.000 description 1
- YSRQSWIJJLAZMI-UHFFFAOYSA-N NCC(C1CCOCC1)O Chemical compound NCC(C1CCOCC1)O YSRQSWIJJLAZMI-UHFFFAOYSA-N 0.000 description 1
- SDXBYMSRSVFAOW-UHFFFAOYSA-N O=C(CBr)C(CC1)CCN1OC(c1ccccc1)=O Chemical compound O=C(CBr)C(CC1)CCN1OC(c1ccccc1)=O SDXBYMSRSVFAOW-UHFFFAOYSA-N 0.000 description 1
- OKHIFRRBGHULIA-UHFFFAOYSA-N OC(CBr)C1CCOCC1 Chemical compound OC(CBr)C1CCOCC1 OKHIFRRBGHULIA-UHFFFAOYSA-N 0.000 description 1
- PBMFSQRYOILNGV-UHFFFAOYSA-N c1ccnnc1 Chemical compound c1ccnnc1 PBMFSQRYOILNGV-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
- C07D487/04—Ortho-condensed systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Definitions
- Epigenetics is the study of heritable changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence. Molecular mechanisms that play a role in epigenetic regulation include DNA methylation and chromatin/histone modifications. Histone methylation, in particular, is critical in many epigenetic phenomena.
- Chromatin the organized assemblage of nuclear DNA and histone proteins, is the basis for a multitude of vital nuclear processes including regulation of transcription, replication, DNA-damage repair and progression through the cell cycle.
- Histones are the chief protein components of chromatin. They act as spools around which DNA winds, and they play a role in gene regulation. There are a total of six classes of histones (HI, H2A, H2B, H3, H4, and H5) organized into two super classes: core histones (H2A, H2B, H3, and H4) and linker histones (HI and H5).
- the basic unit of chromatin is the nucleosome, which consists of about 147 base pairs of DNA wrapped around the histone octamer, consisting of two copies each of the core histones H2A, H2B, H3, and H4 (Luger, et al. (1997) Nature 389:251-260).
- Histones particularly residues of the amino termini of histones H3 and H4 and the amino and carboxyl termini of histones H2A, H2B and HI, are susceptible to a variety of post-translational modifications including acetylation, methylation, phosphorylation, ribosylation, sumoylation, ubiquitination, citrullination, deimination, and biotinylation.
- the core of histones H2A and H3 can also be modified.
- Histone modifications are integral to diverse biological processes such as gene regulation, DNA repair, and chromosome condensation.
- One class of histone methylases is characterized by the presence of a SET domain, comprising about 130 amino acids.
- EZH2 is an example of a human SET-domain containing methylase. EZH2 associates with EED (Embryonic Ectoderm Development) and SUZ12 (suppressor of zeste 12 homolog) to form a complex known as PRC2 (Polycomb Group Repressive Complex 2) having the ability to tri-methylate histone H3 at lysine 27 (Cao and Zhang, Mol. Cell 15:57-67, 2004). PRC2 complexes can also include RBAP46 and RBAP48 subunits. Another example is the related methylase EZH1.
- EZH2 knockdown inhibits tumor growth and metastasis. Recently, it has been shown that down modulation of EZH2 in murine models blocks prostate cancer metastasis (Min et al., "An oncogene-tumor suppressor cascade drives metastatic prostate cancer by coordinately activating Ras and nuclear factor- kappaB," Nat Med. 2010 Mar; 16(3):286-94). EZH2 overexpression is associated with aggressiveness of certain cancers such as breast cancer (Kleer et al., Proc. Nat. Acad. Sci. USA 100: 11606-11611, 2003).
- methyl modifying enzymes in particular EZH2 and mutant forms thereof, are an attractive target for modulation, given their role in the regulation of diverse biological processes. It has now been found that compounds of this invention, and pharmaceutically acceptable compositions thereof, are effective as agents that modulate the activity of EZH2 and, in some cases, EZH1. Such compounds have the general formula I:
- Compounds provided by this invention are also useful for the study of methyl modifying enzymes in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by methyl modifying enzymes and the comparative evaluation of new methyl modifying enzyme modulators.
- the present invention provides a compound of the formula I:
- Z is C(R 9 ) or N
- one of Y 1 or Y 2 is N and the other is C;
- one of X 1 , X 2 , X 3 , or X 4 is N and each of the others is independently C(R 5 );
- each of R 1 , R2 and R 9 is independently selected from hydrogen, halo, -OH, -CN, Ci-C 4 alkyl, -0-(Ci-C 4 alkyl), -N(R 7 ) 2 , -(C 0 -C 4 alkylene)-aryl, -(C 0 -C 4
- R 1 and R 9 are taken together with atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring; or
- R 2" and R 9" are taken together with atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring;
- each of R 3 , R 4 and R 5 is independently selected from hydrogen, halo, -CN, -(C 0 -C 4 alkylene)-R 8 , -(C 2 -C 6 alkenyl or alkynyl)-R 6 , -(Ci-C 4 alkylene)-0-R 6 , -(Ci-C 4
- alkylene 0-(C 1 -C 4 alkylene)-R 8 , -O-(C 0 -C 4 alkylene)-R 6 , -0-(C 2 -C 4
- R is selected from CrC 4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl;
- structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, Z and E double bond isomers, and Z and E conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention.
- a particular enantiomer may, in some embodiments be provided substantially free of the corresponding enantiomer, and may also be referred to as "optically enriched.”
- “Optically-enriched,” as used herein, means that the compound is made up of a significantly greater proportion of one enantiomer. In certain embodiments the compound is made up of at least about 90% by weight of a preferred enantiomer. In other embodiments the compound is made up of at least about 95%, 98%, or 99% by weight of a preferred enantiomer.
- Preferred enantiomers may be isolated from racemic mixtures by any method known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts or prepared by asymmetric syntheses.
- HPLC high pressure liquid chromatography
- Jacques et al. Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen, et al., Tetrahedron 33:2725 (1977); Eliel, E.L. Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); Wilen, S.H. Tables of Resolving Agents and Optical Resolutions p. 268 (E.L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, IN 1972).
- a wavy bond ( ) at a chiral center in a chemical structure is used to denote compounds of the invention that are optically pure, but whose optical rotation has not been determined.
- a straight bond at a chiral center indicates a racemic mixture although, as stated above, the invention also includes all possible isomeric forms of the racemate.
- heteroatom means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), ⁇ (as in pyrrolidinyl) or NR + (as in N- substituted pyrrolidinyl)).
- a "direct bond” or “covalent bond” refers to a single, double or triple bond. In certain embodiments, a “direct bond” or “covalent bond” refers to a single bond.
- halo and halogen as used herein refer to an atom selected from fluorine (fluoro,-F), chlorine (chloro,-Cl), bromine (bromo,-Br), and iodine (iodo,-I).
- aliphatic or "aliphatic group”, as used herein, denotes a hydrocarbon moiety that may be straight-chain (i.e., unbranched), branched, or cyclic (including fused, bridging, and spiro-fused polycyclic) and may be completely saturated or may contain one or more units of unsaturation, but which is not aromatic. Unless otherwise specified, aliphatic groups contain 1-6 carbon atoms. In some embodiments, aliphatic groups contain 1-4 carbon atoms, and in yet other embodiments aliphatic groups contain 1-3 carbon atoms.
- Suitable aliphatic groups include, but are not limited to, linear or branched, alkyl, alkenyl, and alkynyl groups, and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl) alkenyl .
- bivalent C 1-8 (or C 1-6 ) saturated or unsaturated, straight or branched, hydrocarbon chain refers to bivalent alkylene, alkenylene, and alkynylene chains that are straight or branched as defined herein.
- alkylene refers to a bivalent alkyl group.
- An "alkylene chain” is a polymethylene group, ⁇ . ⁇ .,-(03 ⁇ 4) ⁇ -, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3.
- a substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
- alkenylene refers to a bivalent alkenyl group.
- a substituted alkenylene chain is a polymethylene group containing at least one double bond in which one or more hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
- alkynylene refers to a bivalent alkynyl group.
- Co alkylene as used herein means a bond.
- a moiety defined herein as "-(Co-C 6 alkylene)-aryl” includes both -aryl (i.e., Co alkylene-aryl) and -(C -C alkylene)-aryl.
- alkyl refers to a monovalent saturated, straight- or branched-chain hydrocarbon radical derived from an aliphatic moiety containing between one and six carbon atoms by removal of a single hydrogen atom. In some embodiments, alkyl contains 1-5 carbon atoms. In another embodiment, alkyl contains 1-4 carbon atoms. In still other embodiments, alkyl contains 1-3 carbon atoms. In yet another embodiment, alkyl contains 1-2 carbons.
- alkyl radicals include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, sec-pentyl, iso-pentyl, tert-butyl, n-pentyl, neopentyl, n-hexyl, sec-hexyl, and the like.
- alkenyl denotes a monovalent group derived from a straight- or branched-chain aliphatic moiety having at least one carbon-carbon double bond by the removal of a single hydrogen atom.
- alkenyl contains 2-6 carbon atoms.
- alkenyl contains 2-5 carbon atoms.
- alkenyl contains 2-4 carbon atoms.
- alkenyl contains 2-3 carbon atoms.
- Alkenyl groups include, for example, ethenyl ("vinyl”), propenyl ("allyl”), butenyl, l-methyl-2-buten-l-yl, and the like.
- alkynyl refers to a monovalent group derived from a straight- or branched-chain aliphatic moiety having at least one carbon-carbon triple bond by the removal of a single hydrogen atom.
- alkynyl contains 2-6 carbon atoms.
- alkynyl contains 2-5 carbon atoms.
- alkynyl contains 2-4 carbon atoms.
- alkynyl contains 2-3 carbon atoms.
- Representative alkynyl groups include, but are not limited to, ethynyl, 2-propynyl ("propargyl"), 1-propynyl, and the like.
- Carbocyclyl (also referred to herein as “carbocycle” “cycloaliphatic” or “cycloalkyl”), as used herein, means a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but where there is no ring is aromatic.
- aryl used alone or as part of a larger moiety as in “aralkyl”, “aralkoxy”, or “aryloxyalkyl”, refers to monocyclic and bicyclic carbon ring systems having a total of five to 10 ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains three to seven ring members.
- aryl may be used interchangeably with the term “aryl ring”.
- “aryl” refers to an aromatic ring system which includes, but not limited to, phenyl, biphenyl, naphthyl, anthracyl and the like, which may bear one or more substituents.
- aryl is a group in which an aromatic ring is fused to one or more carbocyclyl rings regardless of whether the aromatic carbon ring or the carbocyclic ring is the pendant ring, or a group in which an aromatic carbon ring is fused to one or more heteroaryl or heterocyclyl, rings, such as indanyl, phthalimidyl, naphthimidyl, phenanthridinyl, or tetrahydronaphthyl, and the like, wherein the pendant ring of the fused ring system is the aromatic carbon ring.
- heteroaryl and “heteroar-”, used alone or as part of a larger moiety refer to groups having 5 to 10 ring atoms, preferably 5, 6, or 9 ring atoms; having 6, 10, or 14 ⁇ electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms.
- heteroatom refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quaternized form of a basic nitrogen.
- Heteroaryl groups include, without limitation, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, and pteridinyl.
- heteroaryl and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocyclyl rings, wherein the pendant ring of the fused ring system is heteroaromatic.
- Non-limiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H-quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, 5,6,7, 8-tetrahydroquinolinyl, 5,6,7, 8-tetrahydroisoquinolinyl, and pyrido[2,3-b]-l,4-oxazin-3(4H)-one.
- heteroaryl group may be mono- or bicyclic.
- heteroaryl may be used interchangeably with the terms “heteroaryl ring", “heteroaryl group”, or “heteroaromatic”, any of which terms include rings that are optionally substituted.
- heteroarylkyl refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted.
- heteroarylene refers to a bivalent mono- or bicyclic heteroaryl ring.
- heterocycle As used herein, the terms “heterocycle”, “heterocyclyl”, “heterocyclic radical”, and “heterocyclic ring” are used interchangeably and refer to a stable 4- to 7-membered monocyclic or 7- 10-membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above.
- a “heterocycle”, group is a ⁇ , -heterocyclylene group (i.e., a spiro-fused ring).
- nitrogen When used in reference to a ring atom of a heterocycle, the term "nitrogen” includes a substituted nitrogen.
- the nitrogen in a saturated or partially unsaturated ring having 0-3 heteroatoms selected from oxygen, sulfur or nitrogen, the nitrogen may be N (as in 3,4-dihydro-2H-pyrrolyl), ⁇ (as in pyrrolidinyl), or + NR (as in N- substituted pyrrolidinyl).
- a heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted.
- saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, pyrrolidonyl, piperidinyl, pyrrolinyl, 1,2,3,4-tetrahydroquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and quinuclidinyl.
- heterocycle used interchangeably herein, and also include groups in which a heterocyclyl ring is fused to one or more aryl, heteroaryl, or cycloaliphatic rings, such as indolinyl, 3H-indolyl, chromanyl, phenanthridinyl, 2-azabicyclo[2.2.1]heptanyl, octahydroindolyl, or tetrahydroquinolinyl , wherein the pendant ring of the fused ring system is heterocyclyl.
- heterocyclyl group may be mono- or bicyclic.
- heterocyclylalkyl refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted.
- partially unsaturated refers to a ring moiety that includes at least one double or triple bond between ring atoms but is not aromatic.
- partially unsaturated is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aryl or heteroaryl moieties, as herein defined.
- compounds of the invention may contain "optionally substituted” moieties.
- substituted whether preceded by the term “optionally” or not, means that one or more hydrogens of the designated moiety are replaced with a suitable substituent.
- an "optionally substituted” group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at each position.
- Combinations of substituents envisioned under this invention are preferably those that result in the formation of stable or chemically feasible compounds.
- stable refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes disclosed herein.
- Suitable monovalent substituents on a substitutable carbon atom of an "optionally substituted" group are independently
- R° may be substituted as defined below and is independently hydrogen, Ci_6 aliphatic, -CH 2 Ph, -0(CH 2 )o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R°, taken together with their intervening atom(s), form a 3-12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, which may be substituted as defined below.
- Suitable monovalent substituents on R° are independently halogen, -(CH 2 ) 0 - 2 R*, -(haloR*), -(CH 2 ) 0 _ 2 OH, -(CH 2 ) 0 - 2 OR*, -(CH 2 ) 0 - 2 CH(OR*) 2 ; -O(haloR')
- Suitable divalent substituents that are bound to vicinal substitutable carbons of an "optionally substituted” group include:-0(CR 2 ) 2-3 0-, wherein each independent occurrence of R is selected from hydrogen, C 1-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
- Suitable substituents on the aliphatic group of R * include halogen, -R*, -(haloR*), -OH, -OR*, -O(haloR'), -CN, -C(0)OH, -C(0)OR*, -NH 2 , -NHR*, - NR* 2 , or -N0 2 , wherein each R* is unsubstituted or where preceded by "halo" is substituted only with one or more halogens, and is independently Ci_4 aliphatic, -CH 2 Ph, -0(CH 2 )o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
- each R ⁇ is independently hydrogen, Ci_6 aliphatic which may be substituted as defined below, unsubstituted -OPh, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R ⁇ , taken together with their intervening atom(s) form an unsubstituted 3-12-membered saturated, partially unsaturated, or
- Suitable substituents on the aliphatic group of R ⁇ are independently halogen, -R*, -(haloR*), -OH, -OR*, -O(haloR'), -CN, -C(0)OH, -C(0)OR*, -NH 2 , -NHR*, - NR*2, or -NO2, wherein each R* is unsubstituted or where preceded by "halo" is substituted only with one or more halogens, and is independently C 1 _ 4 aliphatic, -CH 2 Ph, -0(CH 2 )o iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
- an inhibitor is defined as a compound that binds to and /or inhibits a target S-adenosylmethionine (SAM) utilizing enzyme with measurable affinity.
- SAM S-adenosylmethionine
- an inhibitor has an IC 50 and/or binding constant of less about 50 ⁇ , less than about 1 ⁇ , less than about 500 nM, less than about 100 nM, or less than about 10 nM.
- measurable affinity and “measurably inhibit,” as used herein, means a measurable change in activity of at least one SAM utilizing enzyme between a sample comprising a provided compound, or composition thereof, and at least one SAM dependent enzyme, and an equivalent sample comprising at least one SAM dependent enzyme, in the absence of said compound, or composition thereof.
- the present invention provides a compound of formula I:
- one of Y 1 or Y 2 is N and the other is C.
- Y 1 is N and Y2 is C.
- Y 2 is C and Y 2 is N.
- one of X 1 , X 2 , X 3 , or X 4 is N and each of the others is independently C(R 5 ).
- X 1 is N and each of X 2 , X 3 , and X 4 is C(R 5 ).
- X 2 is N and each of X 1 , X 3 , and X 4 is C(R 5 ).
- X 3 is N and each of X 1 , X 2 , and X 4 is C(R 5 ).
- X 4 is N and each of X 1 , X 2 , and X 3 is C(R 5 ).
- Y 1 is N, X 4 is N, and each of X 1 , X2 , and X 3 is C(R 5 ). In another aspect of these embodiments, Y 1 is N, X2 is N, and each of X 1 , X 3 , and X 4 is C(R 5 ). In an alternate aspect of these embodiments, Y 2 is N, X 3 is N, and X 1 , X 2 , and X 4 is C(R 5 ). In a more specific aspect of these embodiments, Y is N, X 4 is N, and X 5 is selected from C(H), C(OCH 3 ) and C(CH 3 ).
- each R 1 and R 2 is independently selected from hydrogen, halo, -OH, -CN, Ci-C 4 alkyl, -0-(d-C 4 alkyl), -N(R 7 ) 2 , -(C 0 -C 4 alkylene)-aryl, -(C 0 -C 4 alkylene)-heteroaryl, -(C 0 -C 4 alkylene)-heterocyclyl, and -(C 0 -C 4 alkylene)-carbocyclyl; or one of R 1 or R2 is taken together with R 9 and the atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring, wherein each R and R 9 are as defined herein.
- R 1 is -CH .
- R2 is selected from -CH 3 , -CH 2 CH 3 , -CH 2 CH 2 CH 3 , -OCH 3 , and -OCHF 2 .
- R 1 is -CH 3 ; and R 2 is selected from -CH 3 , -CH 2 CH 3 , -CH 2 CH 2 CH 3 , -OCH 3 , and -OCHF 2 .
- R 3 , R 4 and each R 5 are independently selected from hydrogen, halo, -CN, -(C 0 -C 4 alkylene)-R , -(C 2 -C 6 alkenyl or
- R 3 is -CH . In some embodiments, R 3 is chloro.
- R 4 is selected
- R 10 is selected from hydrogen and C C 4 alkyl.
- R 4 is additionally selected from -CH(CH )N(R 10 ) 2 .
- any heteroaryl, heterocyclyl, aryl or carbocyclyl portion of R 4 is optionally substituted.
- any heteroaryl, heterocyclyl, aryl or carbocyclyl portion of R 4 is optionally substituted with oxo, methyl, methylsulfonyl, propyl, ethyl, ethylcarbonyl, ethylsulfonyl, or methylcarbonyl, wherein the methyl, ethyl or propyl portion of the substituent is further optionally substituted with up to four substituents independently selected from fluoro, methyl and hydroxy.
- R 4 is selected from l-(l-methylpiperidin-4-yl)ethyl, 5-methyl-isoxazol-4-yl, 3,5-dimethyl- isoxazol-4-yl, l,5-dimethyl-lH-pyrazol-3-yl, l-(l-ethylsulfonylpiperidin-4-yl)ethyl, 1,4- dimethyl-lH-pyrazol-5-yl, 1 -methyl- lH-pyrazol-5-yl, l-(tetrahydropyran-4-yl)ethyl, 1- (pyridin-3-yl)ethyl, l-(methylsulfonylamino)ethyl, l-(l-methyl-2-oxopiperidin-4-yl)ethyl, 1- (methylsulfonyl(N-ethyl)amino)ethyl, 1
- R 4 is selected from l-(l-(2,2-difluoropropanoyl)piperidin-
- R 5 is hydrogen.
- R 3 is -CH 3
- R 5 is hydrogen
- R 4 is selected from l-(l-methylpiperidin-4- yl)ethyl, 5-methyl-isoxazol-4-yl, 3,5-dimethyl-isoxazol-4-yl, l,5-dimethyl-lH-pyrazol-3-yl, l-(l-ethylsulfonylpiperidin-4-yl)ethyl, l,4-dimethyl-lH-pyrazol-5-yl, 1 -methyl- lH-pyrazol-
- each R is independently selected from hydrogen, -CH , -CH 2 CH , -S(0) 2 -CH , and -C(0)CH 3 .
- R is selected from C C 4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl.
- R is Ci-C 4 alkyl.
- R 8 is methyl.
- R 8 is aryl.
- R 8 is optionally substituted phenyl. In some embodiments, R is unsubstituted phenyl. In some embodiments, R 8 is substituted phenyl. In some embodiments, R 8 is optionally substituted heteroaryl. In some embodiments, R 8 is carbocyclyl. In some embodiments, R 8 is optionally substituted heterocyclyl.
- any alkyl, alkylene, alkenyl, alkenylene, alkynyl, alkynylene, aryl, heteroaryl, heterocyclyl or carbocyclyl portion of the compound is optionally substituted.
- the compound of formula I is represented by structural formula II:
- each of R 1 , R2 and R 9 is independently selected from hydrogen, C C 4 alkyl, and -0-(Ci-C 4 alkyl);
- one of Y 1 or Y 2 is N and the other is C;
- one of X 2 , X 3 , or X 4 is N and each of the others is independently CH or -0-(Ci-C 4 alkyl);
- R is selected from hydrogen, halo, and CrC 4 alkyl
- R 4 is selected from -C(0)-R 8 , -(C 0 -C 4 alkylene)-R 8 , and -(C 0 -C 4 alkylene)-N(R 7 ) 2 ;
- R is selected from C C 4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl, wherein each of the aryl, heteroaryl, carbocyclyl and heterocyclyl are optionally substituted.
- R 1 is CrC 4 alkyl
- R 9 is hydrogen
- R is CrC 4 alkyl or -0-(CrC 4 alkyl)
- the optional substituents present on the optionally substituted groups for R are selected independently from one or two groups selected from Ci-C 4 alkyl, R ⁇ , Ci-C 4 alkyl-OH, -S0 2 -C 1 -C 4 alkyl, and -C(0)R ⁇
- R ⁇ is C C 4 alkyl substituted by one or more fluoro.
- Exemplary compounds of formula I are set forth below in Table 1.
- two (or more) of the compounds in Table 1 having one (or more) wavy bonds will have the exact same structure. Because the wavy bond represents a chiral center of undetermined optical rotation, such compounds will be understood to be separate and distinct optical isomers of one another.
- Table 1 is annotated to indicate those sets of two or more compounds that have the same depicted structure, but are of different stereochemistry.
- compositions are provided.
- the invention provides a composition comprising a compound of this invention or a pharmaceutically acceptable derivative thereof and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
- the amount of compound in compositions of this invention is such that is effective to measurably modulate a histone methyl modifying enzyme, or a mutant thereof, in a biological sample or in a patient.
- the amount of compound in compositions of this invention is such that is effective to measurably modulate a histone methyl modifying enzyme, or a mutant thereof, in a biological sample or in a patient.
- a composition of this invention is formulated for administration to a patient in need of such composition. In some embodiments, a composition of this invention is formulated for oral administration to a patient.
- patient means an animal, preferably a mammal, and most preferably a human.
- compositions of this invention refers to a non-toxic carrier, adjuvant, or vehicle that does not destroy the pharmacological activity of the compound with which it is formulated.
- Pharmaceutically acceptable carriers, adjuvants or vehicles that may be used in the compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxyprop
- a "pharmaceutically acceptable derivative” means any non-toxic salt, ester, salt of an ester or other derivative of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
- compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir.
- parenteral as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra- synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques.
- the compositions are administered orally, intraperitoneally or intravenously.
- Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
- the sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol.
- a non-toxic parenterally acceptable diluent or solvent for example as a solution in 1,3-butanediol.
- acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
- sterile, fixed oils are conventionally employed as a solvent or suspending medium.
- any bland fixed oil may be employed including synthetic mono- or di-glycerides.
- Fatty acids such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions.
- These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents that are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions.
- Other commonly used surfactants such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
- compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, aqueous suspensions or solutions.
- carriers commonly used include lactose and corn starch.
- Lubricating agents such as magnesium stearate, are also typically added.
- useful diluents include lactose and dried cornstarch.
- aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
- compositions of this invention may be administered in the form of suppositories for rectal administration.
- suppositories for rectal administration.
- suppositories can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug.
- suitable non-irritating excipient include cocoa butter, beeswax and polyethylene glycols.
- compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs.
- Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches may also be used.
- compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers.
- Carriers for topical administration of compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water.
- provided pharmaceutically acceptable compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers.
- Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
- compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride.
- the pharmaceutically acceptable compositions may be formulated in an ointment such as petrolatum.
- Pharmaceutically acceptable compositions of this invention may also be administered by nasal aerosol or inhalation.
- compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.
- compositions of this invention are formulated for oral administration. Such formulations may be administered with or without food. In some embodiments, pharmaceutically acceptable compositions of this invention are administered without food. In other embodiments, pharmaceutically acceptable compositions of this invention are administered with food.
- compositions of the present invention that may be combined with the carrier materials to produce a composition in a single dosage form will vary depending upon the host treated and the particular mode of administration.
- provided compositions should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of the inhibitor can be administered to a patient receiving these compositions.
- a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated.
- the amount of a compound of the present invention in the composition will also depend upon the particular compound in the composition.
- compounds and compositions described herein are generally useful for the modulating of activity of one or more enzymes involved in epigenetic regulation and in particular EZHl and EZH2 and, even more specifically EZH2 and mutant forms thereof.
- compounds of the present invention down-regulate or suppress the activity of EZH2.
- compounds of the present invention are antagonists of EZH2 activity.
- compounds of the present invention down-regulate or suppress the activity of EZHl.
- compounds of the present invention are antagonists of EZHl activity.
- compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with overexpression of EZH1 or EZH2 and/or expression of a mutant form of EZH2, particularly those mutant forms that alter EZH2 substrate activity.
- EZH2 deletions, missense and frameshift mutations suggest that EZH2 functions as a tumor suppressor in blood disorders such as myelodysplasia syndromes (MDS) and myeloid malignancies (Ernst et al., Nat Genet. 2010 Aug; 42(8):722-6; Nikoloski et al., Nat Genet. 2010 Aug; 42(8):665-7).
- compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with the presence of EZH2 having a Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687 mutation.
- the EZH2 has a VY641N mutation.
- the present invention provides a method of treating a subject suffering from a disease and/or disorder associated with overexpression of EZH1 or EZH2 and/or expression of a mutant form of EZH2 comprising the step of administering a compound or composition of formula I.
- the above method additionally comprises the preliminary step of determining if the subject is overexpressing EZH2 or expressing a mutant form of EZH2.
- the disease or disorder associated with the presence of a mutant form of EZH2 is a human B cell lymphoma.
- the disease and/or disorder associated with the presence of Y641N EZH2 is follicular lymphoma or diffuse large-B-cell lymphoma.
- compounds or compositions of the present invention are useful in treating blood disorders, such as myelodysplasia syndromes, leukemia, anemia and cytopenia.
- compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with cellular proliferation.
- compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with misregulation of cell cycle or DNA repair.
- compounds and compositions of the present invention are useful in treating cancer.
- Exemplary types of cancer include breast cancer, prostate cancer, colon cancer, renal cell carcinoma, glioblastoma multiforme cancer, bladder cancer, melanoma, bronchial cancer, lymphoma and liver cancer.
- the present invention provides a method of reducing the activity of EZH2 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of wide-type EZH2 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of wild-type EZH1 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of a mutant form of EZH2 in a subject comprising the step of administering a compound or composition of formula I.
- the present invention provides a method of reducing the activity of a mutant form of EZH2 in a subject comprising the step of administering a compound or composition of formula I, wherein the mutant form of EZH2 is selected from Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2.
- the mutant form of EZH2 is selected from Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2.
- the present invention provides a method of reducing the activity of a mutant form of EZH2 in a subject comprising the step of administering a compound or composition of formula I, wherein the mutant form of EZH2 is Y641N EZH2.
- the present invention provides a method of treating a subject suffering from a disease and/or disorder associated with EZH2, wherein the method additionally comprises the preliminary step of determining if the subject is expressing a mutant form of EZH2, such as Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2.
- a mutant form of EZH2 such as Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2.
- the present invention provides a method of reducing the activity of a mutant form of EZH2, such as Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2, in a subject in need thereof comprising the step of administering a compound or composition of formula I.
- the present invention provides a method of treating a subject suffering from a disease and/or disorder associated with EZH2, wherein the method additionally comprises the preliminary step of determining if the subject has increased levels of histone H3 Lys-27-specific trimethylation (H3K27me3), as compared to a subject known not to express a mutant form of EZH2.
- H3K27me3 histone H3 Lys-27-specific trimethylation
- a compound when reverse phase HPLC is used to purify a compound, a compound may exist as an acid addition salt. In some embodiments, a compound may exist as a formic acid or mono-, di-, or tri-fluoroacetic acid salt.
- the present invention contemplates individual compounds described herein. Where individual compounds exemplified are isolated and/or characterized as a salt, for example, as a trifluoroacetic acid salt, the present invention contemplates a free base of the salt, as well as other pharmaceutically acceptable salts of the free base.
- Step 1 methy -methyl-l -pyrazole-5-carboxylate:
- Step 3 5-(bromomethyl)-l-methyl-lH-pyrazole:
- Step 1 N, -dimethoxy-N-methylnicotinamide:
- Step 2 l-(2-methoxypyridin-3-yl)ethanone:
- Step 3 3-(2-bromoacetyl)pyridin-2(lH)-one:
- Step 1 2-diazo-l-(tetrahydro-2H-pyran-4-yl)ethanone:
- Tetrahydro-2H-pyran-4-carboxylic acid (10 g,76.84 mmol) was added into a flask and thionyl chloride (20 mL, 275.7 mmol) was added drop wise at 0 °C. The mixture was stir at this temp for 2 h and then the volatiles were removed under vacuum to afford the acid chloride which was directly dissolved in dichloromethane (5 mL) and (trimethylsilyl)diazomethane (22.8g, 0.2 mol) was added drop wise at °C The mixture was stirred for 2 h affording the solution was used directly in the next step.
- Step 2 2-bromo-l- tetrah dro-2H- ran-4- l ethanone:
- Example 5 Synthesis of ethyl 6-methyl-7-(l-methyl-2-oxo-l,2- dihvdropyridine-4-carbonyl)pyrrolori,2-b1pyridazine-5-carboxylate (Intermediate 4.T).
- Step 1 -acetyl-l-methylpyridin-2(lH)-one:
- Step 2 4-(2-bromoacetyl)-l-methylpyridin-2(lH)-one:
- Step 3 ethyl 6-methyl-7-(l-methyl-2-oxo-l,2-dihydropyridine-4- carbon l)pyrrolo[l,2-b]pyridazine-5-carboxylate:
- Step 1 methyl 7-(l-cyclopropyl-l-hydroxyethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylate (Intermediate 5):
- Step 2 methyl 7-(l-cyclopropyl-l-hydroxyethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylate (Intermediate 6):
- Step 1 ethyl 6-methyl-7-(l-(methylamino)ethyl)pyrrolo[l,2-b]pyridazine-5- carboxylate:
- Step 2 ethyl 6-methyl-7-(l-(N-methylmethylsulfonamido)ethyl)pyrrolo[l,2- b ]pyridazin -5-carboxylate:
- Example 10 Synthesis of ethyl 6-methyl-7-(l-(l-methyl-2-oxo-l,2- dihvdropyridin-3-yl)ethyl)pyrrolo[l,2-b1pyridazine-5-carboxylate (Intermediate 11).
- Step 1 ethyl 6-methyl-7-(l-methyl-2-oxo-l,2-dihydropyridine-3- carbonyl)pyrrolo l,2-b]pyridazine-5-carboxylate (9):
- Step 2 ethyl 6-methyl-7-(l-(l-methyl-2-oxo-l,2-dihydropyridin-3- yl)vinyl)pyrrolo[ -b ]pyridazine-5-carboxylate (10):
- Step 3 ethyl 6-methyl-7-(l-(l-methyl-2-oxo-l,2-dihydropyridin-3- yl)ethyl)pyrrolo[ -b ]pyridazine-5-carboxylate ( 11 ):
- Lithium hydroxide monohydrate (41 mg, 0.97 mmol) was added to ethyl 7-acetyl-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (40 mg, 0.16 mmol) in THF/H 2 0 (10 mL, 1: 1) and the resulting mixture was heated to reflux with stirring overnight. The mixture was then allowed to cool to rt and was cone, in vacuo, diluted with water (5 mL), and acidified with IN HCl to a pH ⁇ 2. A precipitate formed which was collected via vacuum filtration to afford the title compound as a white solid (30 mg, yield 85 ).
- Step 2 2,4-dihydroxy-6-methylnicotinonitrile:
- Step 5 tert-butyl ((2,4-dimethoxy-6-methylpyridin-3-yl)methyl)carbamate:
- Step 1 4,6-dimethyl-2-oxo-l,2-dihydropyridine-3-carbonitrile:
- Step 2 tert-butyl ((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)carbamate:
- Step 3 3-(aminomethyl)-4,6-dimethylpyridin-2(lH)-one (16):
- Example 17 Synthesis of (R or S)-7-(l-(N-ethylmethylsulfonamido)ethyl)-N- ((4-methoxy-6-methyl-2-oxo-l,2-dihvdropyridin-3-yl)methyl)-6-methylpyrrolori,2- blpyridazine-5-carboxamide (Peak 1; Compound 107) and (R or S)-7-(l-(N- ethylmethylsulfonamido)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihvdropyridin-3- yl)methyl)-6-methylpyrrolori,2-b1pyridazine-5-carboxamide (Peak 2; Compound 108):
- Peak 1 - Compund 107 Peak 2- Compound 108
- Example 18 Synthesis of 7-(l-(l-(2,2-difluoropropanoyl)piperidin-4- yl)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihvdropyridin-3-yl)methyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 135).
- Step 1 Synthesis of 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[ 1,2-b ]pyridazine-5-carboxylic acid.
- Step 2 Synthesis of benzyl 4-(l-(5-(((4-methoxy-6-methyl-2- dihydropyridin-3-yl)methyl)carbamoyl)-6-methylpyrrolo[l,2-b]pyridazin-7- yl)ethyl)piperidine-l-carboxylate.
- Step 3 Synthesis of N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxam
- Example 19 Synthesis of N-((4-methoxy-6-methyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-6-methyl-7-(l-(l-(3 rifluoropropyl)piperidin-4- yl)ethyl)pyrrolori,2-b1pyridazine-5-carboxamide (Compound 134).
- Step 1 Synthesis of ethyl 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin- -yl)ethyl)pyrrolo[ 1,2-b ]pyridazine-5-carboxylate.
- Step 2 Synthesis of 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b ]pyridazine-5-carbox lic aci
- Step 3 Synthesis of N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(l-(l-(3,3,3 rifluoropropyl)piperidin-4-yl)ethyl)pyrrolo[l,2- b Ipyridazin e- 5-carboxam ide.
- Example 20 Synthesis of ethyl 7-(l-(l-(2-fluoro-2- methylpropanoyl)piperidin-4-yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Compound 144).
- Step 1 Synthesis of ethyl 7-(l-(l-(2-fluoro-2-methylpropanoyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Intermediate 17 ) .
- Step 2 Synthesis of ethyl 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylat (Intermediate 18A).
- Step 3 Synthesis of 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (Intermediate 19A).
- Step 4 Synthesis of 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4- yl)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 144).
- Example 21 Synthesis of 6-chloro-N-((4,6-dimethyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-7-(l-(l-(2-hvdroxy-2-methylpropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 141).
- Step 1 Synthesis of ethyl 7-(l-((benzyloxy)carbonyl)piperidine-4- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- Step 2 Synthesis of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)-l- hydroxyethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- Step 3 Synthesis of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- Step 4 Synthesis of ethyl 7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine- - carboxylate.
- Step 5 Synthesis of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- Step 6 Synthesis of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)- -dichloropyrrolo[ 1,2-b ]pyridazine-5-carboxylate.
- Step 7 Synthesis of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)- -chloropyrrolo[l,2-b]pyridazine-5-carboxylate.
- Step 8 Synthesis of ethyl 6-chloro-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2- b ]pyridazine-5-carboxylate
- Step 9 Synthesis of ethyl 6-chloro-7-(l-(l-(2-hydroxy-2- methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- Step 10 Synthesis of 6-chloro-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin- -yl)ethyl)pyrrolo[ 1,2-b Jpyridazine-5-carboxylic acid.
- Step 11 Synthesis of 6-chloro-N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2- b]pyridazine-5-carboxamid (Compound 141).
- Step 1 Synthesis of 7-tert-butyl 5-ethyl 6-methylpyrrolo[l,2-b]pyridazine- 5 7-dicarboxylate
- Step 2 Synthesis of 5-(ethoxycarbonyl)-6-methylpyrrolo[l,2-b]pyridazine-7- rboxylic acid
- Step 3 Synthesis of ethyl 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (Intermediate 20 A)
- Step 4 Synthesis of 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylic acid (Intermediate 21 A)
- Step 5 Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxamide
- 5-carboxylic acid (96.0 mg, 0.33 mmol) in N,N-dimethylformamide (2.5 mL) was added O- (7-azabenzotriazol-l-yl)-N,N,N,N,-tetramethyluronium (250.8 mg, 0.66 mmol), N,N- diisopropylethylamine (127.7 mg, 0.99 mmol) and 3-(aminomethyl)-4,6-dimethylpyridin- 2(lH)-one (150.7 mg, 0.99 mmol). The resulting solution was stirred at room temperature under N 2 overnight. The mixture was partitioned between ethyl acetate and water.
- Example 23 Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihvdropyridin-3- yl)methyl)-6-methyl-7-(4-(methylsulfonyl)piperazine-l-carbonyl)pyrrolori,2- blpyridazine-5-carboxamide (Compound 126)
- Step 1 Synthesis of ethyl 6-methyl-7-(piperazine-l-carbonyl)pyrrolo[l,2- b ]pyridazine-5-carboxylate
- Step 2 Synthesis of ethyl 6-methyl-7-(4-(methylsulfonyl)piperazine-l- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxyl te
- Step 3 Synthesis of 6-Methyl-7-(4-(methylsulfonyl)piperazine-l- carbonyl)pyrrolo[l,2-b ]pyridazine-5-carboxylic acid
- Step 4 Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(4-(methylsulfonyl)piperazine-l-carbonyl)pyrrolo[l,2-b]pyridaz -carboxamide (Compound 126)
- Step 1 Synthesis of ethyl 2,6-dimethyl-7-(l-(piperidin-4- yl)ethyl)pyrrolo[l,2-b ]pyridazine-5-carboxylate (Intermediate 21 A )
- Step 2 Synthesis of ethyl 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2, 6-dimethylpyrrolo[l,2-b Jpyridazine-5-carboxylate (Intermediate 22 A )
- Step 3 Synthesis of 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (Intermediate 23 A)
- Step 4 Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-6-methylpyrro b Ipyridazin e-5- carb oxam ide (Compound 129)
- Example 25 Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihvdropyridin-3- yl)methyl)-7-(l-(l-(2-hvdroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2-methoxy-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 127):
- Step 1 Synthesis of ethyl 2-methoxy-6-methyl-7-(l-(l-(2- oxopropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate
- Step 2 Synthesis of ethyl 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2-methoxy-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate
- Step 3 Synthesis of 7-(l-(l-(2-Hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2-methoxy-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid
- Step 4 Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2-methoxy-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 127).
- Example 26 Synthesis of 7-(l-(dimethylamino)ethyl)-N-((4-methoxy-6- methyl-2-oxo-l,2-dihvdropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b1pyridazine-5- carboxamide (Compound 130)
- Step 1 Synthesis of Ethyl 7-(l-(dimethylamino)ethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylate
- the intermediates shown in the following table were prepared using intermediate 4G and the appropriate amine according to the procedure described in Step 1 of Example
- Step 1A Synthesis of ethyl 7-(l-(ethyl(methyl)amino)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Intermediate 25B)
- Step 2 Synthesis of 7-(l-(Dimethylamino)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-
- Step 3 Synthesis of 7-(l-(dimethylamino)ethyl)-N-((4-methoxy-6-methyl-2- oxo-l,2-dihydropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxam
- Example 27 Synthesis of 6-chloro-N-((4-methoxy-6-methyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-7-(l-(tetrahvdro-2H-pyran-4-yl)ethyl)pyrrolo[l,2- blpyridazine-5-carboxamide (Compound 132)
- Steps 1-3 Synthesis of ethyl 7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- 2-bromo-l-(tetrahydro-2H-pyran-4- yl)ethanone (from Example 3, step 2) was combined with methyl but-2-ynoate (3) and pyridazine (2) as described in Example 4 to produce ethyl 7-(tetrahydro-2H-pyran-4- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- That intermediate was treated with methylmagnesium bromide in THF as described in Example 8, step 1 to produce ethyl 7-(l- hydroxy-l-(tetrahydro-2H-pyran-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
- the hydroxyl intermediate was then treated with Et SiH dissolved in THF, followed by TFA, as described in Example 8, step 2 to produce ethyl 7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[ 1 ,2-b]pyridazine-5-carboxylate.
- Step 4 Synthesis of ethyl 3,6-dichloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate
- Step 5 Synthesis of ethyl 6-chloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate
- Step 6 Synthesis of 6-chloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b ]pyridazine-5-carboxylic acid
- Step 7 Synthesis of 6-chloro-N-((4-methoxy-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)-7-(l-(tetrahydro-2H-pyran-4-yl)ethyl)pyrrolo[l,2-
- Example 28 IC3 ⁇ 4n measurements for Inhibitors using EZH2.
- EZH2 Assays were carried out by mixing rPRC2 together with biotinylated oligonucleosome substrates in the presence of the radio-labeled enzyme co-factor, S-adenosyl-L-methionine ( H SAM) (Perkin Elmer) and monitoring the enzymatically mediated transfer of tritiated methyl groups from H SAM to histone lysine residues.
- H SAM S-adenosyl-L-methionine
- the amount of resulting tritiated methyl histone product was measured by first capturing the biotinylated oligonucleosomes in streptavidin (SAV) coated FlashPlates (Perkin Elmer), followed by a wash step to remove unreacted H SAM, and then counting on a TopCount NXT 384 well plate scintillation counter (Perkin Elmer).
- SAV streptavidin
- the final assay conditions for EZH2 were as follows: 50 mM Tris Buffer pH 8.5, 1 mM DTT, 69 ⁇ Brij-35 detergent, 5.0 mM MgCl 2 , 0.1 mg/mL BSA, 0.2 ⁇ 3 H SAM, 0.2 ⁇ biotinylated oligonucleosomes, 3.6 ⁇ H3K27me3 peptide and 2 nM EZH2.
- Compound IC 50 measurements were obtained as follows: Compounds were first dissolved in 100% DMSO as 10 mM stock solutions. Ten point dose response curves were generated by dispensing varying amounts of the 10 mM compound solution in 10 wells of the 384 well plate (Echo; Labcyte), pure DMSO was then used to backfill the wells to insure all wells have the same amount of DMSO. A 12.5 ⁇ ⁇ volume of the HMT enzyme, H3K27me3 peptide and oligonucleosome substrate in assay buffer was added to each well of the assay plate using a Multidrop Combi (ThermoFisher).
- ICso's were computed using a 4 parameter fit non-linear curve fitting software package (XLFIT, part of the database package, ActivityBase (IDBS)) where the four parameters were IC 50 , Hill slope, pre-transitional baseline (0% INH), and post-transitional baseline (100% INH); with the latter two parameters being fixed to zero and 100%, respectively, by default.
- XLFIT 4 parameter fit non-linear curve fitting software package
- IDBS ActivityBase
- Table 2 shows the activity of selected compounds of this invention in the EZH2 and Y641N EZH2 activity inhibition assay.
- IC 50 values are reported as follows: “A” indicates an IC 50 value of less than 100 nM; “B” indicates an IC 50 value of 100 nM to 1 ⁇ ; “C” indicates an IC 50 value of greater than 1 ⁇ and less than 10 ⁇ for each enzyme; “D” indicates an IC 50 value of greater than 10 ⁇ for each enzyme; and "*(X ⁇ )” indicates that no inhibition was observed at the highest concentration (i.e., X ⁇ ) of compound tested.
- EXAMPLE 29 ECsn MEASUREMENTS FOR INHIBITORS IN HELA
- H3K27me3 MSD Hela Assay Trypsinized HeLa cells were counted and diluted in 10% DMEM (Life Technologies, Cat. # 10569) to 5000 cells/75 Seventy-five ⁇ of cells were place in each well of a 96-well flat-bottomed plate and incubated at 37°C for 4 hours. Twenty-five ⁇ of test compound (at various concentrations) was added to the cells and incubation continued at 37°C for 96 hours. Media was then removed and the cells rinsed once with ice cold PBS.
- the emptied wells were rinsed with 150uL ice-cold 20mM Tris pH 7.5, ImM EDTA, lmM EGTA, supplemented with fresh lx Protease Inhibitor cocktail and ImM AEBSF ("NO salt NO detergent buffer) and transferred to the respective wells in the new plate. Three hundred ⁇ of NO Salt NO detergent buffer was then added to each well of lysates and the plates frozen at -80°C.
- H3 antibodies were obtained from Cell Signalling (Cat #s 4499 and 9733).
- the goat anti -rabbit antibody was obtained from Meso-Scale Discovery (Cat #R32AB-1).
- H3K27me3 Alpha Hela Assay (AlphaLISA). Ten different doses of each test compound (in a series of 3-fold dilutions) were plated in duplicate 384-well tissue culture treated plates (Catalog # 781080; Greiner Bio One; Monroe, North Carolina). Hela cells grown in culture were trypsinized and counted using a Countess® cell counter (Catalog # CI 0281 ; Life Technologies, Grand Island, NY). Cell were diluted to 67,000 cells per mL inl0% DMEM (Catalog # 10569-010 Life Technologies, Grand Island, NY) and 15 ⁇ (1,000 cells) were plated into each well using the Biotek MicroFloTM Select Dispenser (BioTek Instruments, Inc. Vermont, USA),) of the 384-well plate. Plates were incubated at 37°C 15% C0 2 for 72 hrs. One of the duplicate plates was processed for HeLa assay and the other for viability.
- Table 3 shows the activity of selected compounds of this invention in the two different HeLa cell assays described above.
- EC 50 values are reported as follows: “A” indicates an EC 50 value of less than 400 nM; “B” indicates an EC 50 value of 400 nM to 2 ⁇ ; “C” indicates an EC 50 value of greater than 2 ⁇ and less than 10 ⁇ for each enzyme; “D” indicates an EC 50 value of greater than 10 ⁇ for each enzyme; and "*(X ⁇ )” indicates that no inhibition was observed at the highest concentration (i.e., X ⁇ ) of compound tested.
- Karpas-422 GI50 Assay Ten different doses of each test compound (in a series of 3-fold dilutions) are plated in 384-well tissue culture treated plates (Catalog # 781080; Greiner Bio One; Monroe, North Carolina). Karpas-422 cells grown in suspension culture are counted using a Countess® cell counter (Catalog # C10281 ; Life Technologies, Grand Island, NY). Cells are diluted to 25,000 cells per mL in 10% RPMI (Catalog # 61870-036 Life Technologies, Grand Island, NY) and 45 ⁇ L ⁇ (1 ,125 cells) are plated into each well using the Biotek MicroFloTM Select Dispenser (BioTek Instruments, Inc. Vermont, USA),) of the 384-well plate. Plates were incubated at 37°C 15% C0 2 for 168 hrs (7 days).
- Cell viability is assayed by adding 30 of Cell Titer Glo ((Catalog #G7571 Promega Madison, WI) to each well with cells with media. The plates are incubated at RT for 30 minutes on a plate shaker at low speed. The plates are then read using an En Vision-Alpha Reader (model # 2104 Perkin Elmer; Waltham, MA).
- Cell Titer Glo (Catalog #G7571 Promega Madison, WI)
- Data from the assay is analyzed using Assay Assistant (Constellation Pharmaceuticals In-house product) and Activity Base (IDBS Ltd, Surrey, UK) template. Data files are imported to Assay Assistant and assay conditions are specified. A unique Analysis ID is created and the data files exported to Activity Base. An analysis template is created on Activity Base to measure dose-dependent cell viability. Readout of DMSO wells are used to normalize the data. Resulting curves are fitted using Activity base software Model 205 (IDBS Ltd, Surrey, UK). The data is checked for quality, validated and integrated in excel format using SARview (IDBS Ltd, Surrey, UK).
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
Abstract
Agents for modulating methyl modifying enzymes, compositions and uses thereof are provided herein.
Description
MODULATORS OF METHYL MODIFYING ENZYMES, COMPOSITIONS AND
USES THEREOF
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to U.S. Provisional Application No. 61/790,566, filed March 15, 2013, the entire contents of which are incorporated herein by reference.
BACKGROUND OF THE INVENTION
[0002] Epigenetics is the study of heritable changes in gene expression caused by mechanisms other than changes in the underlying DNA sequence. Molecular mechanisms that play a role in epigenetic regulation include DNA methylation and chromatin/histone modifications. Histone methylation, in particular, is critical in many epigenetic phenomena.
[0003] Chromatin, the organized assemblage of nuclear DNA and histone proteins, is the basis for a multitude of vital nuclear processes including regulation of transcription, replication, DNA-damage repair and progression through the cell cycle. A number of factors, such as chromatin-modifying enzymes, have been identified that play an important role in maintaining the dynamic equilibrium of chromatin (Margueron, et al. (2005) Curr. Opin. Genet. Dev. 15: 163-176).
[0004] Histones are the chief protein components of chromatin. They act as spools around which DNA winds, and they play a role in gene regulation. There are a total of six classes of histones (HI, H2A, H2B, H3, H4, and H5) organized into two super classes: core histones (H2A, H2B, H3, and H4) and linker histones (HI and H5). The basic unit of chromatin is the nucleosome, which consists of about 147 base pairs of DNA wrapped around the histone octamer, consisting of two copies each of the core histones H2A, H2B, H3, and H4 (Luger, et al. (1997) Nature 389:251-260).
[0005] Histones, particularly residues of the amino termini of histones H3 and H4 and the amino and carboxyl termini of histones H2A, H2B and HI, are susceptible to a variety of post-translational modifications including acetylation, methylation, phosphorylation, ribosylation, sumoylation, ubiquitination, citrullination, deimination, and biotinylation. The core of histones H2A and H3 can also be modified. Histone modifications are integral to diverse biological processes such as gene regulation, DNA repair, and chromosome condensation.
[0006] One class of histone methylases is characterized by the presence of a SET domain, comprising about 130 amino acids. EZH2 is an example of a human SET-domain containing methylase. EZH2 associates with EED (Embryonic Ectoderm Development) and SUZ12 (suppressor of zeste 12 homolog) to form a complex known as PRC2 (Polycomb Group Repressive Complex 2) having the ability to tri-methylate histone H3 at lysine 27 (Cao and Zhang, Mol. Cell 15:57-67, 2004). PRC2 complexes can also include RBAP46 and RBAP48 subunits. Another example is the related methylase EZH1.
[0007] The oncogenic activities of EZH2 have been shown by a number of studies. In cell line experiments, over-expression of EZH2 induces cell invasion, growth in soft agar, and motility while knockdown of EZH2 inhibits cell proliferation and cell invasion (Kleer et al., 2003, Proc. Nat. Acad. Sci. USA 100: 11606-11611; Varambally et al., (2002), "The polycomb group protein EZH2 is involved in progression of prostate cancer," Nature 419, 624-629). It has been shown that EZH2 represses the expression of several tumor suppressors, including E-cadherin, DAB2IP and RUNX3 among others. In xenograft models, EZH2 knockdown inhibits tumor growth and metastasis. Recently, it has been shown that down modulation of EZH2 in murine models blocks prostate cancer metastasis (Min et al., "An oncogene-tumor suppressor cascade drives metastatic prostate cancer by coordinately activating Ras and nuclear factor- kappaB," Nat Med. 2010 Mar; 16(3):286-94). EZH2 overexpression is associated with aggressiveness of certain cancers such as breast cancer (Kleer et al., Proc. Nat. Acad. Sci. USA 100: 11606-11611, 2003). Recent studies also suggest that prostate cancer specific oncogenic fusion gene TMPRSS2-ERG induces repressive epigenetic programs via direct activation of EZH2 (Yu et al., "An Integrated Network of Androgen Receptor, Polycomb, and TMPRSS2-ERG Gene Fusions in Prostate Cancer Progression," Cancer Cell. 2010 May 18;17(5):443-454).
SUMMARY OF THE INVENTION
[0008] The present disclosure encompasses the recognition that methyl modifying enzymes, in particular EZH2 and mutant forms thereof, are an attractive target for modulation, given their role in the regulation of diverse biological processes. It has now been found that compounds of this invention, and pharmaceutically acceptable compositions thereof, are effective as agents that modulate the activity of EZH2 and, in some cases, EZH1. Such compounds have the general formula I:
, or a pharmaceutically acceptable salt or tautomer thereof, wherein each variable is as defined herein.
[0009] Compounds of the present invention, and pharmaceutically acceptable compositions thereof, are useful for treating a variety of diseases, disorders or conditions, associated with a methyl modifying enzyme. Such diseases, disorders, or conditions include those described herein.
[0010] Compounds provided by this invention are also useful for the study of methyl modifying enzymes in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by methyl modifying enzymes and the comparative evaluation of new methyl modifying enzyme modulators.
DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS
1. General Description of Compounds of the Invention
1. In certain embodiments, the present invention provides a compound of the formula I:
harmaceutically acceptable salt or tautomer thereof, wherein:
Z is C(R9) or N;
one of Y 1 or Y 2 is N and the other is C;
one of X1, X2, X3, or X4 is N and each of the others is independently C(R5);
each of R 1 , R2 and R 9 is independently selected from hydrogen, halo, -OH, -CN, Ci-C4 alkyl, -0-(Ci-C4 alkyl), -N(R7)2, -(C0-C4 alkylene)-aryl, -(C0-C4
alkylene)-heteroaryl, -(C0-C4 alkylene)-heterocyclyl, and -(C0-C4 alkylene)-carbocyclyl; or
R1 and R9 are taken together with atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring; or
R 2" and R 9" are taken together with atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring;
each of R3, R4 and R5 is independently selected from hydrogen, halo, -CN, -(C0-C4 alkylene)-R8, -(C2-C6 alkenyl or alkynyl)-R6, -(Ci-C4 alkylene)-0-R6, -(Ci-C4
alkylene)-0-(C1-C4 alkylene)-R8, -O-(C0-C4 alkylene)-R6, -0-(C2-C4
alkylene)-0-R8, -0-(Ci-C4 alkylene)-R6, -(C0-C4 alkylene)-N(R7)2, -(C0-C4
alkylene)-C(0)-0-R6, -(C0-C4 alkylene)-0-C(0)-R6, -(C0-C4 alkylene)-C(0)-N(R7)2, -(C0-C4 alkylene)-N(R7)-C(0)-R6, -0-(Ci-C4 alkylene)-C(0)-N(R7)2, -0-(C2-C4
alkylene)-N(R7)-C(0)-(R7), -(C0-C4 alkylene)-S(0)-R8, -(C0-C4 alkylene)-S(0)2-R8, -(C0-C4 alkylene)-S(0)2-N(R7)2, -(C0-C4 alkylene)-N(R7)-S(0)2-R8, and -(C0-C4 alkylene)-C(0)-R8 each R6 is independently selected from hydrogen or R8;
each R7 is independently selected from -(C0-C4 alkylene)-R6, -(C0-C4 alkylene)-0-R6, -S(0)2-R8, -C(=0)-R8, -C(=0)-N(R6)2, -(Ci-C4 alkylene)-0-C(=0)-R8 and -(Co-C4 alkylene)-C(=0)-0-R6; or
two R are taken together with the nitrogen atom to which they are commonly bound to form an optionally substituted heterocyclyl or heteroaryl;
R is selected from CrC4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl;
wherein unless otherwise designated any alkyl, alkylene, alkenyl, alkynyl, aryl, heteroaryl, heterocyclyl or carbocyclyl portion of the compound is optionally substituted.
2. Compounds and Definitions
[0011] Definitions of specific functional groups and chemical terms are described in more detail below. For purposes of this invention, the chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75th Ed., inside cover, and specific functional groups are generally defined as described therein. Additionally, general principles of organic chemistry, as well as specific functional moieties and reactivity, are described in Organic Chemistry, Thomas Sorrell, University Science Books, Sausalito, 1999; Smith and March March's Advanced Organic Chemistry, 5th Edition, John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive Organic Transformations, VCH Publishers, Inc., New York, 1989; Carruthers, Some Modern
Methods of Organic Synthesis, 3 rd Edition, Cambridge University Press, Cambridge, 1987; the entire contents of each of which are incorporated herein by reference.
[0012] Unless otherwise stated, structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, Z and E double bond isomers, and Z and E conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention. Additionally, unless otherwise stated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures including the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13C- or 14C-enriched carbon are within the scope of this invention. Such compounds are useful, for example, as analytical tools, as probes in biological assays, or as therapeutic agents in accordance with the present invention.
[0013] Where a particular enantiomer is preferred, it may, in some embodiments be provided substantially free of the corresponding enantiomer, and may also be referred to as "optically enriched." "Optically-enriched," as used herein, means that the compound is made up of a significantly greater proportion of one enantiomer. In certain embodiments the compound is made up of at least about 90% by weight of a preferred enantiomer. In other embodiments the compound is made up of at least about 95%, 98%, or 99% by weight of a preferred enantiomer. Preferred enantiomers may be isolated from racemic mixtures by any method known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts or prepared by asymmetric syntheses. See, for example, Jacques et al., Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen, et al., Tetrahedron 33:2725 (1977); Eliel, E.L. Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); Wilen, S.H. Tables of Resolving Agents and Optical Resolutions p. 268 (E.L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, IN 1972).
[0014] A wavy bond ( ) at a chiral center in a chemical structure is used to denote compounds of the invention that are optically pure, but whose optical rotation has not been determined. A straight bond at a chiral center indicates a racemic mixture although, as stated above, the invention also includes all possible isomeric forms of the racemate.
[0015] The term "heteroatom" means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or
silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), ΝΗ (as in pyrrolidinyl) or NR+ (as in N- substituted pyrrolidinyl)).
[0016] As used herein a "direct bond" or "covalent bond" refers to a single, double or triple bond. In certain embodiments, a "direct bond" or "covalent bond" refers to a single bond.
[0017] The terms "halo" and "halogen" as used herein refer to an atom selected from fluorine (fluoro,-F), chlorine (chloro,-Cl), bromine (bromo,-Br), and iodine (iodo,-I).
[0018] The term "aliphatic" or "aliphatic group", as used herein, denotes a hydrocarbon moiety that may be straight-chain (i.e., unbranched), branched, or cyclic (including fused, bridging, and spiro-fused polycyclic) and may be completely saturated or may contain one or more units of unsaturation, but which is not aromatic. Unless otherwise specified, aliphatic groups contain 1-6 carbon atoms. In some embodiments, aliphatic groups contain 1-4 carbon atoms, and in yet other embodiments aliphatic groups contain 1-3 carbon atoms. Suitable aliphatic groups include, but are not limited to, linear or branched, alkyl, alkenyl, and alkynyl groups, and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl) alkenyl .
[0019] The term "unsaturated", as used herein, means that a moiety has one or more units of unsaturation.
[0020] As used herein, the term "bivalent C1-8 (or C1-6) saturated or unsaturated, straight or branched, hydrocarbon chain", refers to bivalent alkylene, alkenylene, and alkynylene chains that are straight or branched as defined herein.
[0021] The term "alkylene" refers to a bivalent alkyl group. An "alkylene chain" is a polymethylene group, ί.ε.,-(0¾)η-, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3. A substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
[0022] The term "alkenylene" refers to a bivalent alkenyl group. A substituted alkenylene chain is a polymethylene group containing at least one double bond in which one or more hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
[0023] The term "alkynylene" refers to a bivalent alkynyl group.
[0024] The term "Co alkylene" as used herein means a bond. Thus, a moiety defined herein as "-(Co-C6 alkylene)-aryl" includes both -aryl (i.e., Co alkylene-aryl) and -(C -C alkylene)-aryl.
[0025] The term "alkyl," as used herein, refers to a monovalent saturated, straight- or branched-chain hydrocarbon radical derived from an aliphatic moiety containing between one and six carbon atoms by removal of a single hydrogen atom. In some embodiments, alkyl contains 1-5 carbon atoms. In another embodiment, alkyl contains 1-4 carbon atoms. In still other embodiments, alkyl contains 1-3 carbon atoms. In yet another embodiment, alkyl contains 1-2 carbons. Examples of alkyl radicals include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, sec-pentyl, iso-pentyl, tert-butyl, n-pentyl, neopentyl, n-hexyl, sec-hexyl, and the like.
[0026] The term "alkenyl," as used herein, denotes a monovalent group derived from a straight- or branched-chain aliphatic moiety having at least one carbon-carbon double bond by the removal of a single hydrogen atom. In certain embodiments, alkenyl contains 2-6 carbon atoms. In certain embodiments, alkenyl contains 2-5 carbon atoms. In some embodiments, alkenyl contains 2-4 carbon atoms. In another embodiment, alkenyl contains 2-3 carbon atoms. Alkenyl groups include, for example, ethenyl ("vinyl"), propenyl ("allyl"), butenyl, l-methyl-2-buten-l-yl, and the like.
[0027] The term "alkynyl," as used herein, refers to a monovalent group derived from a straight- or branched-chain aliphatic moiety having at least one carbon-carbon triple bond by the removal of a single hydrogen atom. In certain embodiments, alkynyl contains 2-6 carbon atoms. In certain embodiments, alkynyl contains 2-5 carbon atoms. In some embodiments, alkynyl contains 2-4 carbon atoms. In another embodiment, alkynyl contains 2-3 carbon atoms. Representative alkynyl groups include, but are not limited to, ethynyl, 2-propynyl ("propargyl"), 1-propynyl, and the like.
[0028] The term "carbocyclyl" (also referred to herein as "carbocycle" "cycloaliphatic" or "cycloalkyl"), as used herein, means a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but where there is no ring is aromatic.
[0029] The term "aryl" used alone or as part of a larger moiety as in "aralkyl", "aralkoxy", or "aryloxyalkyl", refers to monocyclic and bicyclic carbon ring systems having a total of five to 10 ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains three to seven ring members. The term "aryl" may be used interchangeably with the term "aryl ring". In certain embodiments of the present
invention, "aryl" refers to an aromatic ring system which includes, but not limited to, phenyl, biphenyl, naphthyl, anthracyl and the like, which may bear one or more substituents. Also included within the scope of the term "aryl", as it is used herein, is a group in which an aromatic ring is fused to one or more carbocyclyl rings regardless of whether the aromatic carbon ring or the carbocyclic ring is the pendant ring, or a group in which an aromatic carbon ring is fused to one or more heteroaryl or heterocyclyl, rings, such as indanyl, phthalimidyl, naphthimidyl, phenanthridinyl, or tetrahydronaphthyl, and the like, wherein the pendant ring of the fused ring system is the aromatic carbon ring.
[0030] The terms "heteroaryl" and "heteroar-", used alone or as part of a larger moiety, e.g., "heteroaralkyl", or "heteroaralkoxy", refer to groups having 5 to 10 ring atoms, preferably 5, 6, or 9 ring atoms; having 6, 10, or 14 π electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms. The term "heteroatom" refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quaternized form of a basic nitrogen. Heteroaryl groups include, without limitation, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, and pteridinyl. The terms "heteroaryl" and "heteroar-", as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocyclyl rings, wherein the pendant ring of the fused ring system is heteroaromatic. Non-limiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H-quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, 5,6,7, 8-tetrahydroquinolinyl, 5,6,7, 8-tetrahydroisoquinolinyl, and pyrido[2,3-b]-l,4-oxazin-3(4H)-one. A heteroaryl group may be mono- or bicyclic. The term "heteroaryl" may be used interchangeably with the terms "heteroaryl ring", "heteroaryl group", or "heteroaromatic", any of which terms include rings that are optionally substituted. The term "heteroaralkyl" refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted. The term "heteroarylene" refers to a bivalent mono- or bicyclic heteroaryl ring.
[0031] As used herein, the terms "heterocycle", "heterocyclyl", "heterocyclic radical", and "heterocyclic ring" are used interchangeably and refer to a stable 4- to 7-membered monocyclic or 7- 10-membered bicyclic heterocyclic moiety that is either saturated or
partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above. In certain embodiments, a "heterocycle", group is a Ι, -heterocyclylene group (i.e., a spiro-fused ring). When used in reference to a ring atom of a heterocycle, the term "nitrogen" includes a substituted nitrogen. As an example, in a saturated or partially unsaturated ring having 0-3 heteroatoms selected from oxygen, sulfur or nitrogen, the nitrogen may be N (as in 3,4-dihydro-2H-pyrrolyl), ΝΗ (as in pyrrolidinyl), or +NR (as in N- substituted pyrrolidinyl).
[0032] A heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted. Examples of such saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, pyrrolidinyl, pyrrolidonyl, piperidinyl, pyrrolinyl, 1,2,3,4-tetrahydroquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and quinuclidinyl. The terms "heterocycle", "heterocyclyl", "heterocyclyl ring", "heterocyclic group", "heterocyclic moiety", and "heterocyclic radical", are used interchangeably herein, and also include groups in which a heterocyclyl ring is fused to one or more aryl, heteroaryl, or cycloaliphatic rings, such as indolinyl, 3H-indolyl, chromanyl, phenanthridinyl, 2-azabicyclo[2.2.1]heptanyl, octahydroindolyl, or tetrahydroquinolinyl , wherein the pendant ring of the fused ring system is heterocyclyl. A heterocyclyl group may be mono- or bicyclic. The term "heterocyclylalkyl" refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted.
[0033] As used herein, the term "partially unsaturated" refers to a ring moiety that includes at least one double or triple bond between ring atoms but is not aromatic. The term "partially unsaturated" is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aryl or heteroaryl moieties, as herein defined.
[0034] As described herein, compounds of the invention may contain "optionally substituted" moieties. In general, the term "substituted", whether preceded by the term "optionally" or not, means that one or more hydrogens of the designated moiety are replaced with a suitable substituent. Unless otherwise indicated, an "optionally substituted" group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at each position. Combinations of substituents envisioned under this invention are preferably those
that result in the formation of stable or chemically feasible compounds. The term "stable", as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes disclosed herein.
[0035] Suitable monovalent substituents on a substitutable carbon atom of an "optionally substituted" group are independently
halogen; -(CH2)0-4R0; -(CH2)0-4OR°; -O-(CH2)0^C(O)OR°; -(CH2)0-4CH(OR°)2; -(CH2)0-4SR°; -(CH2)o-4Ph, which may be substituted with R°; -(CH2)o-40(CH2)o-iPh which may be substituted with R°; -CH=CHPh, which may be substituted with
R°; -N02; -CN; -N3; -(CH2)0-4N(R°)2; -(CH2)0-4N(R°)C(O)R0; -N(R°)C(S)R°; -(CH2)0-4N(R°)C (0)NR°2; -N(R°)C(S)NR°2; -(CH2)0-4N(R°)C(O)OR0; -N(R°)N(R°)C(0)R°; -N(R°)N(R°)C(0) NR°2; -N(R°)N(R°)C(0)OR°; -(CH2)0-4C(O)R°; -C(S)R°; -(CH2)0-4C(O)OR°; -(CH2)0-4C(O)S R°; -(CH2)o-4C(0)OSiR°3; -(CH2)0-4OC(O)R°; -OC(O)(CH2)0_4SR-; -SC(S)SR°; -(CH2)0_4SC( 0)R°; -(CH2)o-4C(0)NR°2; -C(S)NR°2; -C(S)SR°; -SC(S)SR°; -(CH2)0-4OC(O)NR°2; -C(0)N( OR°)R°; -C(0)C(0)R°; -C(0)CH2C(0)R°; -C(NOR°)R°; -(CH2)0^SSR°; -(CH2)0-4S(O)2R°; -( CH2)o^S(0)2OR°; -(CH2)0-4OS(O)2R°; -S(0)2NR°2; -(CH2)0-4S(O)R°; -N(R°)S(0)2NR°2; -N(R °)S(0)2R°; -N(OR°)R°; -C(NH)NR°2; -P(0)2R°; -P(0)R°2; -OP(0)R°2; -OP(0)(OR°)2; -SiR°3; -(C1-4 straight or branched alkylene)0-N(R°)2; or -(C1-4 straight or branched
alkylene)C(0)0-N(R°)2, wherein each R° may be substituted as defined below and is independently hydrogen, Ci_6 aliphatic, -CH2Ph, -0(CH2)o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R°, taken together with their intervening atom(s), form a 3-12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, which may be substituted as defined below.
[0036] Suitable monovalent substituents on R° (or the ring formed by taking two independent occurrences of R° together with their intervening atoms), are independently halogen, -(CH2)0-2R*, -(haloR*), -(CH2)0_2OH, -(CH2)0-2OR*, -(CH2)0-2CH(OR*)2; -O(haloR')
-CN, -N3, -(CH2)o-2C(0)R*, -(CH2)o-2C(0)OH, -(CH2)0-2C(O)OR*, -(CH2)0-2SR*, -(CH2)0_2S H, -(CH2)o-2NH2, -(CH2)o-2NHR*, -(CH2)0-2NR*2, -N02, -SiR*3, -OSiR*3, -C(0)SR*, -(CM straight or branched alkylene)C(0)OR*, or -SSR* wherein each R* is unsubstituted or where preceded by "halo" is substituted only with one or more halogens, and is independently
selected from Ci_4 aliphatic, -CH2Ph, -O(CH2)0_iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents on a saturated carbon atom of R° include =0 and =S.
[0037] Suitable divalent substituents on a saturated carbon atom of an "optionally substituted" group include the following: =0, =S, =NNR* 2, =NNHC(0)R*, =NNHC(0)OR*, =NNHS(0)2R*, =NR*, =NOR*,-0(C(R* 2))2_30-, or-S(C(R* 2))2-3S-, wherein each independent occurrence of R is selected from hydrogen, Ci_6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents that are bound to vicinal substitutable carbons of an "optionally substituted" group include:-0(CR 2)2-30-, wherein each independent occurrence of R is selected from hydrogen, C1-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0038] Suitable substituents on the aliphatic group of R* include halogen, -R*, -(haloR*), -OH, -OR*, -O(haloR'), -CN, -C(0)OH, -C(0)OR*, -NH2, -NHR*, - NR*2, or -N02, wherein each R* is unsubstituted or where preceded by "halo" is substituted only with one or more halogens, and is independently Ci_4 aliphatic, -CH2Ph, -0(CH2)o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0039] Suitable substituents on a substitutable nitrogen of an "optionally substituted" group
include-R†, -NR† 2, -C(0)R†, -C(0)OR†, -C(0)C(0)R†, -C(0)CH2C(0)R†, -S(0)2R†, -S(0)2N R† 2, -C(S)NR† 2, -C(NH)NR† 2, or -N(R†)S(0)2R†; wherein each R† is independently hydrogen, Ci_6 aliphatic which may be substituted as defined below, unsubstituted -OPh, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R†, taken together with their intervening atom(s) form an unsubstituted 3-12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0040] Suitable substituents on the aliphatic group of R† are independently halogen, -R*, -(haloR*), -OH, -OR*, -O(haloR'), -CN, -C(0)OH, -C(0)OR*, -NH2, -NHR*, -
NR*2, or -NO2, wherein each R* is unsubstituted or where preceded by "halo" is substituted only with one or more halogens, and is independently C1_4aliphatic, -CH2Ph, -0(CH2)o iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
[0041] As used herein, the term "inhibitor" is defined as a compound that binds to and /or inhibits a target S-adenosylmethionine (SAM) utilizing enzyme with measurable affinity. In certain embodiments, an inhibitor has an IC50 and/or binding constant of less about 50 μΜ, less than about 1 μΜ, less than about 500 nM, less than about 100 nM, or less than about 10 nM.
[0042] The terms "measurable affinity" and "measurably inhibit," as used herein, means a measurable change in activity of at least one SAM utilizing enzyme between a sample comprising a provided compound, or composition thereof, and at least one SAM dependent enzyme, and an equivalent sample comprising at least one SAM dependent enzyme, in the absence of said compound, or composition thereof.
3. Description of Exemplary Compounds
[0043] In certain embodiments, the present invention provides a compound of formula I:
I
or a pharmaceutically acceptable salt or tautomer thereof, wherein each variable is as defined above and described herein.
[0044] As defined generally above and herein, Z is =C(R9)- or =N-, wherein R9 is as defined above and described herein. In some embodiments, Z is =C(R9)- wherein R9 is as defined above and described herein. In some embodiments, Z is =CH-. In some embodiments, Z is =N-.
[0045] As defined generally above and herein, one of Y 1 or Y 2 is N and the other is C. In some embodiments, Y 1 is N and Y2 is C. In some embodiments Y 2 is C and Y 2 is N.
[0046] As defined generally above and herein, one of X1, X2, X3, or X4 is N and each of the others is independently C(R5). In some embodiments, X1 is N and each of X2, X3, and X4
is C(R5). In some embodiments, X2 is N and each of X1, X3, and X4 is C(R5). In some embodiments, X3 is N and each of X1, X2, and X4 is C(R5). In some embodiments, X4 is N and each of X1, X2, and X3 is C(R5). In one aspect of these embodiments, Y1 is N, X4 is N, and each of X 1 , X2 , and X 3 is C(R 5 ). In another aspect of these embodiments, Y 1 is N, X2 is N, and each of X1, X3, and X4 is C(R5). In an alternate aspect of these embodiments, Y2 is N, X3 is N, and X1, X2, and X4 is C(R5). In a more specific aspect of these embodiments, Y is N, X4 is N, and X5 is selected from C(H), C(OCH3) and C(CH3).
[0047] As defined generally above and herein, each R 1 and R 2 is independently selected from hydrogen, halo, -OH, -CN, Ci-C4 alkyl, -0-(d-C4 alkyl), -N(R7)2, -(C0-C4 alkylene)-aryl, -(C0-C4 alkylene)-heteroaryl, -(C0-C4 alkylene)-heterocyclyl, and -(C0-C4 alkylene)-carbocyclyl; or one of R 1 or R2 is taken together with R 9 and the atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring, wherein each R and R 9 are as defined herein. In some embodiments, R 1 is -CH . In some embodiments, R2 is selected from -CH3, -CH2CH3, -CH2CH2CH3, -OCH3, and -OCHF2. In some embodiments, R1 is -CH3; and R2 is selected from -CH3, -CH2CH3, -CH2CH2CH3, -OCH3, and -OCHF2.
[0048] As defined generally above and herein, R3, R4 and each R5 are independently selected from hydrogen, halo, -CN, -(C0-C4 alkylene)-R , -(C2-C6 alkenyl or
alkynyl)-R6, -(C C4 alkylene)-0-R6, -(C C4 alkylene)-0-(Ci-C4 alkylene)-R8, -O-(C0-C4 alkylene)-R6, -0-(C2-C4 alkylene)-0-R8, -0-(CrC4 alkylene)-R6, -(C0-C4
alkylene)-N(R7)2, -(C0-C4 alkylene)-C(0)-0-R6, -(C0-C4 alkylene)-0-C(0)-R6, -(C0-C4 alkylene)-C(0)-N(R7)2, -(C0-C4 alkylene)-N(R7)-C(0)-R6, -0-(Ci-C4
alkylene)-C(0)-N(R7)2, -0-(C2-C4 alkylene)-N(R7)-C(0)-(R7), -(C0-C4
alkylene)-S(0)-R8, -(C0-C4 alkylene)-S(0)2-R8, -(C0-C4 alkylene)-S(0)2-N(R7)2, -(C0-C4 alkylene)-N(R7)-S(0)2-R8, and -(C0-C4 alkylene)-C(0)-R8, wherein R6, each R7, and R8 are as defined herein. In some embodiments, R 3 is -CH . In some embodiments, R 3 is chloro.
[0049] In some embodiments, R4 is selected
from -heteroaryl, -CH(CH3)-heterocyclyl, -CH(CH3)-heteroaryl, -CH(CH3)-aryl, -CH(CH3)-c arbocyclyl, -CH(CH3)-N(R10)-S(O)2-(C1-C4 alkyl), and -CH(CH3)-N(R10)-C(O)-(C1-C4 alkyl), wherein R10 is selected from hydrogen and C C4 alkyl. In some embodiments, R4 is additionally selected from -CH(CH )N(R10)2. In a specific aspect of these embodiments, any heteroaryl, heterocyclyl, aryl or carbocyclyl portion of R4 is optionally substituted. In a more specific aspect of these embodiments, any heteroaryl, heterocyclyl, aryl or carbocyclyl portion of R4 is optionally substituted with oxo, methyl, methylsulfonyl, propyl, ethyl, ethylcarbonyl, ethylsulfonyl, or methylcarbonyl, wherein the methyl, ethyl or propyl portion
of the substituent is further optionally substituted with up to four substituents independently selected from fluoro, methyl and hydroxy. In a more specific aspect of these embodiments, R4 is selected from l-(l-methylpiperidin-4-yl)ethyl, 5-methyl-isoxazol-4-yl, 3,5-dimethyl- isoxazol-4-yl, l,5-dimethyl-lH-pyrazol-3-yl, l-(l-ethylsulfonylpiperidin-4-yl)ethyl, 1,4- dimethyl-lH-pyrazol-5-yl, 1 -methyl- lH-pyrazol-5-yl, l-(tetrahydropyran-4-yl)ethyl, 1- (pyridin-3-yl)ethyl, l-(methylsulfonylamino)ethyl, l-(l-methyl-2-oxopiperidin-4-yl)ethyl, 1- (methylsulfonyl(N-ethyl)amino)ethyl, 1 -(methylsulfonyl(N-methyl)amino)ethyl, 1 - phenylethyl, l-(methylcarbonyl(N-methyl)amino)ethyl, and 1-cyclopropylethyl.
[0050] In some embodiments, R4 is selected from l-(l-(2,2-difluoropropanoyl)piperidin-
4- yl)ethyl, 1 -( 1 -(2,2-difluoroethanoyl)piperidin-4-yl)ethyl, 1 -( 1 -(2,2,2- trifluoroethyl)piperidin-4-yl)ethyl, l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl, 1- (l-(2,2,2-trifluoroethanoyl)piperidin-4-yl)ethyl, l-(l-(2-fluoro-2-methylpropyl)piperidin-4- yl)ethyl, l-(l-(2,2-difluoropropyl)piperidin-4-yl)ethyl, 1-(1 -(3,3,3- trifluoropropanoyl)piperidin-4-yl)ethyl, l-(l-(3,3,3-trifluoropropyl)piperidin-4-yl)ethyl, 1-(1- (2,2-difluoroethyl)piperidin-4-yl)ethyl, l-(dimethylamino)ethyl, morpholine-4-carbonyl, 4- methylsulfonylpiperazin- 1 -ylcarbonyl, 1 -(tetrahydro-2H-pyran-4-yl)ethyl, 1 -( 1 -(2-fluoro-2- methylpropanoyl)piperidin-4-yl)ethyl, and 1 -(ethyl(methyl)amino)ethyl.
[0051] In some embodiments, R5 is hydrogen. In a more specific aspect of these embodiment, R3 is -CH3, R5 is hydrogen; and R4 is selected from l-(l-methylpiperidin-4- yl)ethyl, 5-methyl-isoxazol-4-yl, 3,5-dimethyl-isoxazol-4-yl, l,5-dimethyl-lH-pyrazol-3-yl, l-(l-ethylsulfonylpiperidin-4-yl)ethyl, l,4-dimethyl-lH-pyrazol-5-yl, 1 -methyl- lH-pyrazol-
5- yl, l-(tetrahydropyran-4-yl)ethyl, l-(pyridin-3-yl)ethyl, l-(methylsulfonylamino)ethyl, 1- (l-methyl-2-oxopiperidin-4-yl)ethyl, l-(methylsulfonyl(N-ethyl)amino)ethyl, 1- (methylsulfonyl(N-methyl)amino)ethyl, 1 -phenylethyl, l-(methylcarbonyl(N- methyl)amino)ethyl, and 1-cyclopropylethyl
[0052] As defined generally above and herein, each R7 is independently selected from -(Co-C4 alkylene)-R6, -(C0-C4 alkylene)-0-R6, -S(0)2-R8, -C(=0)-R8, -C(=0)-N(R6)2, -(C C4 alkylene)-0-C(=0)-R8 and -(Co-C4 alkylene)-C(=0)-0-R6; or two R7 are taken together with the nitrogen atom to which they are commonly bound to form an optionally substituted heterocyclyl or heteroaryl, wherein R 6 and R 8 are as defined herein. In some embodiments, each R 7 is independently selected from -(C0-C4 alkylene)-R 6 , -S(0)2-R 8 , and -C(=0)-R 8. In a more specific aspect, each R is independently selected from hydrogen, -CH , -CH2CH , -S(0)2-CH , and -C(0)CH3.
[0053] As defined generally above and herein, R is selected from C C4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl. In some embodiments, R is Ci-C4 alkyl. In some embodiments, R 8 is methyl. In some embodiments, R 8 is aryl. In some embodiments, R 8 is optionally substituted phenyl. In some embodiments, R is unsubstituted phenyl. In some embodiments, R 8 is substituted phenyl. In some embodiments, R 8 is optionally substituted heteroaryl. In some embodiments, R 8 is carbocyclyl. In some embodiments, R 8 is optionally substituted heterocyclyl.
[0054] Unless otherwise designated, any alkyl, alkylene, alkenyl, alkenylene, alkynyl, alkynylene, aryl, heteroaryl, heterocyclyl or carbocyclyl portion of the compound is optionally substituted.
[0055] In some embodiments, the compound of formula I is represented by structural formula II:
or a pharmaceutically acceptable salt or tautomer thereof, wherein:
each of R 1 , R2 and R 9 is independently selected from hydrogen, C C4 alkyl, and -0-(Ci-C4 alkyl);
one of Y 1 or Y 2 is N and the other is C;
one of X 2 , X 3 , or X 4 is N and each of the others is independently CH or -0-(Ci-C4 alkyl);
R is selected from hydrogen, halo, and CrC4 alkyl;
R4 is selected from -C(0)-R8, -(C0-C4 alkylene)-R8, and -(C0-C4 alkylene)-N(R7)2; R7 is selected from hydrogen, C C4 alkyl, -S(0)2-Ci-C4 alkyl, and -C(=0)-Ci-C4 alkyl; or
two R are taken together with the nitrogen atom to which they are commonly bound to form an optionally substituted heterocyclyl; and
R is selected from C C4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl, wherein each of the aryl, heteroaryl, carbocyclyl and heterocyclyl are optionally substituted.
[0056] In some embodiments, in the compound of formula II, R7 is selected from hydrogen, C C4 alkyl, -S(0)2-C1-C4 alkyl, and -C(=0)-Ci-C4 alkyl; or two R7 are taken
together with the nitrogen atom to which they are commonly bound to form an optionally substituted morpholinyl or piperizinyl; and R is selected from C C4 alkyl, optionally substituted phenyl, optionally substituted cyclopropyl, optionally substituted pyrazolyl, optionally substituted pyridinyl, optionally substituted pyridinyl-2(lH)-one, optionally substituted tetrahydropyranyl, optionally substituted isoxazolyl, and optionally substituted piperidinyl.
[0057] In some embodiments, in the compound of formula II, R1 is CrC4 alkyl; R9 is hydrogen; R is CrC4 alkyl or -0-(CrC4 alkyl); the optional substituents present on the optionally substituted groups for R are selected independently from one or two groups selected from Ci-C4 alkyl, R†, Ci-C4 alkyl-OH, -S02-C1-C4 alkyl, and -C(0)R†; and R† is C C4 alkyl substituted by one or more fluoro.
[0058] It will be understood by those of skill in the art that the compounds of the invention are limited to compounds that are stable.
[0059] Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention.
[0060] Exemplary compounds of formula I are set forth below in Table 1. In some cases two (or more) of the compounds in Table 1 having one (or more) wavy bonds will have the exact same structure. Because the wavy bond represents a chiral center of undetermined optical rotation, such compounds will be understood to be separate and distinct optical isomers of one another. Table 1 is annotated to indicate those sets of two or more compounds that have the same depicted structure, but are of different stereochemistry.
Table 1. Exemplary Compounds of Formula I.
Compound No. Structure
*Compounds 105 and 106 are isolated enantiomers of one another whose optical rotation has not been determined.
**Compounds 107 and 108 are isolated enantiomers of one another whose optical rotation has not been determined.
4. Uses, Formulation and Administration
Pharmaceutically acceptable compositions
[0061] According to another embodiment, the invention provides a composition comprising a compound of this invention or a pharmaceutically acceptable derivative thereof and a pharmaceutically acceptable carrier, adjuvant, or vehicle. The amount of compound in compositions of this invention is such that is effective to measurably modulate a histone methyl modifying enzyme, or a mutant thereof, in a biological sample or in a patient. In certain embodiments, the amount of compound in compositions of this invention is such that is effective to measurably modulate a histone methyl modifying enzyme, or a mutant thereof, in a biological sample or in a patient.
[0062] In certain embodiments, a composition of this invention is formulated for administration to a patient in need of such composition. In some embodiments, a composition of this invention is formulated for oral administration to a patient.
[0063] The term "patient," as used herein, means an animal, preferably a mammal, and most preferably a human.
[0064] The term "pharmaceutically acceptable carrier, adjuvant, or vehicle" refers to a non-toxic carrier, adjuvant, or vehicle that does not destroy the pharmacological activity of the compound with which it is formulated. Pharmaceutically acceptable carriers, adjuvants or vehicles that may be used in the compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium
chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat.
[0065] A "pharmaceutically acceptable derivative" means any non-toxic salt, ester, salt of an ester or other derivative of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
[0066] Compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir. The term "parenteral" as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra- synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques. Preferably, the compositions are administered orally, intraperitoneally or intravenously. Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium.
[0067] For this purpose, any bland fixed oil may be employed including synthetic mono- or di-glycerides. Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents that are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions. Other commonly used surfactants, such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
[0068] Pharmaceutically acceptable compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules,
tablets, aqueous suspensions or solutions. In the case of tablets for oral use, carriers commonly used include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried cornstarch. When aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
[0069] Alternatively, pharmaceutically acceptable compositions of this invention may be administered in the form of suppositories for rectal administration. These can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug. Such materials include cocoa butter, beeswax and polyethylene glycols.
[0070] Pharmaceutically acceptable compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs.
[0071] Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches may also be used.
[0072] For topical applications, provided pharmaceutically acceptable compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers. Carriers for topical administration of compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water. Alternatively, provided pharmaceutically acceptable compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
[0073] For ophthalmic use, provided pharmaceutically acceptable compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride. Alternatively, for ophthalmic uses, the pharmaceutically acceptable compositions may be formulated in an ointment such as petrolatum.
[0074] Pharmaceutically acceptable compositions of this invention may also be administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.
[0075] Most preferably, pharmaceutically acceptable compositions of this invention are formulated for oral administration. Such formulations may be administered with or without food. In some embodiments, pharmaceutically acceptable compositions of this invention are administered without food. In other embodiments, pharmaceutically acceptable compositions of this invention are administered with food.
[0076] The amount of compounds of the present invention that may be combined with the carrier materials to produce a composition in a single dosage form will vary depending upon the host treated and the particular mode of administration. Preferably, provided compositions should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of the inhibitor can be administered to a patient receiving these compositions.
[0077] It should also be understood that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated. The amount of a compound of the present invention in the composition will also depend upon the particular compound in the composition.
Uses of Compounds and Pharmaceutically Acceptable Compositions
[0078] Compounds and compositions described herein are generally useful for the modulating of activity of one or more enzymes involved in epigenetic regulation and in particular EZHl and EZH2 and, even more specifically EZH2 and mutant forms thereof. In some embodiments, compounds of the present invention down-regulate or suppress the activity of EZH2. In some embodiments, compounds of the present invention are antagonists of EZH2 activity. In some embodiments, compounds of the present invention down-regulate or suppress the activity of EZHl. In some embodiments, compounds of the present invention are antagonists of EZHl activity.
[0079] In some embodiments, compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with overexpression of EZH1 or EZH2 and/or expression of a mutant form of EZH2, particularly those mutant forms that alter EZH2 substrate activity. The study of EZH2 deletions, missense and frameshift mutations suggest that EZH2 functions as a tumor suppressor in blood disorders such as myelodysplasia syndromes (MDS) and myeloid malignancies (Ernst et al., Nat Genet. 2010 Aug; 42(8):722-6; Nikoloski et al., Nat Genet. 2010 Aug; 42(8):665-7). In some embodiments, compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with the presence of EZH2 having a Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687 mutation. In a particular aspect of this embodiment, the EZH2 has a VY641N mutation.
[0080] In some embodiments, the present invention provides a method of treating a subject suffering from a disease and/or disorder associated with overexpression of EZH1 or EZH2 and/or expression of a mutant form of EZH2 comprising the step of administering a compound or composition of formula I. In some embodiments, the above method additionally comprises the preliminary step of determining if the subject is overexpressing EZH2 or expressing a mutant form of EZH2.
[0081] In some embodiment, the present invention the disease or disorder associated with the presence of a mutant form of EZH2 is a human B cell lymphoma. In some embodiments, the disease and/or disorder associated with the presence of Y641N EZH2 is follicular lymphoma or diffuse large-B-cell lymphoma. In some embodiments, compounds or compositions of the present invention are useful in treating blood disorders, such as myelodysplasia syndromes, leukemia, anemia and cytopenia. Sneeringer et al., "Coordinated activities of wild-type plus mutant EZH2 drive tumor- associated hypertrimethylation of lysine 27 on histone H3 (H3K27) in human B-cell lymphomas," Proceedings of the National Academy of Sciences, PNAS Early Edition published ahead of print on November 15, 2010.
[0082] In some embodiments, compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with cellular proliferation. In some embodiments, compounds and compositions of the present invention are useful in treating diseases and/or disorders associated with misregulation of cell cycle or DNA repair. In some embodiments, compounds and compositions of the present invention are useful in treating cancer. Exemplary types of cancer include breast cancer, prostate cancer, colon cancer, renal
cell carcinoma, glioblastoma multiforme cancer, bladder cancer, melanoma, bronchial cancer, lymphoma and liver cancer.
[0083] In some embodiments, the present invention provides a method of reducing the activity of EZH2 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of wide-type EZH2 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of wild-type EZH1 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of a mutant form of EZH2 in a subject comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of reducing the activity of a mutant form of EZH2 in a subject comprising the step of administering a compound or composition of formula I, wherein the mutant form of EZH2 is selected from Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2. Each of these mutations alter the EZH2 substrate activity, and thus facilitate the conversion from a di- to a tri-methylated K27 state. In a more specific aspect, the present invention provides a method of reducing the activity of a mutant form of EZH2 in a subject comprising the step of administering a compound or composition of formula I, wherein the mutant form of EZH2 is Y641N EZH2.
[0084] In some embodiments, the present invention provides a method of treating a subject suffering from a disease and/or disorder associated with EZH2, wherein the method additionally comprises the preliminary step of determining if the subject is expressing a mutant form of EZH2, such as Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2. In some embodiments, the present invention provides a method of reducing the activity of a mutant form of EZH2, such as Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V EZH2, in a subject in need thereof comprising the step of administering a compound or composition of formula I. In some embodiments, the present invention provides a method of treating a subject suffering from a disease and/or disorder associated with EZH2, wherein the method additionally comprises the preliminary step of determining if the subject has increased levels of histone H3 Lys-27-specific trimethylation (H3K27me3), as compared to a subject known not to express a mutant form of EZH2.
EQUIVALENTS
[0085] The representative examples that follow are intended to help illustrate the invention, and are not intended to, nor should they be construed to, limit the scope of the invention. Indeed, various modifications of the invention and many further embodiments thereof, in addition to those shown and described herein, will become apparent to those skilled in the art from the full contents of this document, including the examples that follow and the references to the scientific and patent literature cited herein. It should further be appreciated that the contents of those cited references are incorporated herein by reference to help illustrate the state of the art.
[0086] It will be appreciated that for compound preparations described herein, when reverse phase HPLC is used to purify a compound, a compound may exist as an acid addition salt. In some embodiments, a compound may exist as a formic acid or mono-, di-, or tri-fluoroacetic acid salt.
[0087] It will further be appreciated that the present invention contemplates individual compounds described herein. Where individual compounds exemplified are isolated and/or characterized as a salt, for example, as a trifluoroacetic acid salt, the present invention contemplates a free base of the salt, as well as other pharmaceutically acceptable salts of the free base.
[0088] The following examples contain important additional information, exemplification and guidance that can be adapted to the practice of this invention in its various embodiments and the equivalents thereof
EXAMPLES
[0089] As depicted in the Examples below, in certain exemplary embodiments, compounds are prepared according to the following general procedures. It will be appreciated that, although the synthetic methods and Schemes depict the synthesis of certain compounds of the present invention, the following methods and other methods known to one of ordinary skill in the art can be applied to all compounds and subclasses and species of each of these compounds, as described herein.
[0090] Unless otherwise noted, all solvents, chemicals, and reagents were obtained commercially and used without purification. The 1H NMR spectra were obtained in CDC13, J6-DMSO, CD3OD, or J6-acetone at 25°C at 300 MHz on an OXFORD (Varian) with chemical shift (δ, ppm) reported relative to TMS as an internal standard. HPLC-MS
chromatograms and spectra were obtained with Shimadzu LC-MS-2020 system. Chiral analysis and purification were obtained with Yilite P270.
[0091] Example 1. Synthesis of 5-(bromomethyl)-l-methyl-lH-pyrazole (Intermediate 1A)
To a stirred solution of 1 -methyl- lH-pyrazole-5-carboxylic acid (5 g, 39.7 mmol) in methanol (100 mL) was added thionyl chloride (103.2 mmol) under argon atmosphere at 0 °C. The reaction mixture was stirred to room temperature over 12 h. The reaction mixture was cone, in vacuo, then diluted with water (100 mL), carefully quenched with sat'd aqueous NaHCC"3, and extracted with ethyl acetate (100 mL x 3). The combined organic phase was dried with anhydrous sodium sulphate, filtered and the filtrate was concentrated to give afford the title compound as a white solid which was used without further purification (5 g, 90 %) m/z 240.
To a stirred solution of methyl l-methyl-lH-pyrazole-5-carboxylate (3 g, 21.4 mmol) in tetrahydrofuran (50 mL) at 0 °C was added lithium aluminum hydride (977 mg, 25.7 mmol). The mixture was stirred at room temperature for 12 h. The reaction was quenched by adding water, extracted with ethyl acetate (100 mL), dried, concentrated and the residue was purified by column chromatography on silica gel (petroleum ether/ acetic ester =10: 1), to afford the title compound as an off-white oil (2 g, 83.3%).
[0094] The examples shown in the following table were prepared according to the procedure described in Steps 1 and 2 of this Example using the appropriate starting materials and modifications.
To a solution of (l-methyl-lH-pyrazol-5-yl)methanol (500 mg, 4.46 mmol) in anhydrous dichloromethane (20 mL) was added PBr3 (1.21g, 4.46 mmol) at 0 °C. The mixture was purged with nitrogen and stirred at room temperature for 12 h. The reaction mixture was adjusted to pH -8.5 with saturated aqueous sodium bicarbonate, and then was extracted with dichloromethane (10 mL x 3). The combined organic phase was dried by anhydrous sodium sulphate, filtered and concentrated. The residue was purified by preparative TLC (petroleum ether/ acetic ester =5: 1) to afford the title compound an off-white oil (500 mg, 64%) m/z 174.
[0096] The intermediates shown in the following table were prepared according to the procedure described in Step 3 of this Example using the appropriate starting materials and modifications.
[0097] The bromoalkyl products made using the procedures set forth in this example are employed as starting materials for the syntheses set forth in Example 4.
[0098] Example 2. Synthesis of 3-(2-bromoacetyl)pyridin-2(lH)-one (Intermediate
ID):
[0099] Step 1: N, -dimethoxy-N-methylnicotinamide:
A mixture of 2-methoxynicotinic acid (3.5 g, 22.86 mmol), Ν,Ο-dimethylhydroxylamine (2.09 g, 34.28 mmol), HATU (13.04 g, 34.28 mmol) and Hunig's Base (8.86 g, 68.57 mmol) in dichloromethane (50 mL) was stirred at room temperature for overnight. The reaction was then cone, in vacuo and the residue was partitioned between water (20 mL) and ethyl acetate
(20 mL), the organic layer was dried and concentrated to afford the crude title compound as a yellow solid which was used directly without further purification (3 g, 67% yield).
[00100] Step 2: l-(2-methoxypyridin-3-yl)ethanone:
To a solution of N,2-dimethoxy-N-methylnicotinamide (3 g, 0.19 mmol) in tetrahydrofuran (30 mL) was added methylmagnesium bromide (1M solution in tetrahydrofuran, 30.58 mL, 30.58 mmol) at 0 °C. The mixture was stirred at 0 °C to room temperature overnight. The reaction was then quenched with water and extracted with ethyl acetate. The organic layer was dried with MgS04, filtered and concentrated to afford the crude title compound which was used directly in the next step (1.2 g yellow oil, 52 % yield).
[00101] The intermediates shown in the following table were prepared according to the procedure described in Steps 1 and 2 of this Example using the appropriate starting materials and modifications.
[00103] Step 3: 3-(2-bromoacetyl)pyridin-2(lH)-one:
To a solution of l-(2-methoxypyridin-3-yl)ethanone (1 g, 6.62 mmol) in HBr/HOAc (30%, 20 mL) was added bromine (1.06 g, 6.62 mmol) at room temperature. The mixture was stirred at 60 °C for 4 h. It was then cooled to room temperature and methyl tert-butyl ether (20 mL) was added to the mixture. A precipitate formed which was collected via vacuum filtration, collected and dried in vacuo to afford the title compound as a yellow solid. (1 g, 70 % yield).
[00104] The bromoalkyl products made using the procedures set forth in this example are employed as starting materials for the syntheses set forth in Example 4.
[00105] Example 3. Synthesis of 2-bromo-l-(tetrahvdro-2H-pyran-4-yl)ethanone (Intermediate IE)
Tetrahydro-2H-pyran-4-carboxylic acid (10 g,76.84 mmol) was added into a flask and thionyl chloride (20 mL, 275.7 mmol) was added drop wise at 0 °C. The mixture was stir at this temp for 2 h and then the volatiles were removed under vacuum to afford the acid chloride which was directly dissolved in dichloromethane (5 mL) and (trimethylsilyl)diazomethane (22.8g, 0.2 mol) was added drop wise at °C The mixture was stirred for 2 h affording the solution was used directly in the next step.
[00107] The intermediates shown in the following table were prepared according to the procedure described in Step 1 of this Example using the appropriate starting materials and modifications.
[00108] Step 2: 2-bromo-l- tetrah dro-2H- ran-4- l ethanone:
To a solution of 2-diazo-l-(tetrahydro-2H-pyran-4-yl)ethanone was added hydrogen bromide- acetic acid (5.6 g, 40 mmol) drop wise at -10 °C. The mixture was allowed to warm to rt with stirring for 24 h. The reaction was then quenched with saturated aqueous sodium hydrogen carbonate and the mixture was extracted with dichloromethane (80 mL x 3). The combined organic phase was dried over anhydrous sodium sulfate and then filtered. The filtrate was concentrated and the residue was purified by column chromatography on silica gel (eluent: petroleum ether/ethyl acetate = 20/1) to afford the title compound as a brown crystalline solid (10.6 g, 66.6%).
[00109] The intermediates shown in the following table were prepared according to the procedure described in Step 2 of this Example using the appropriate starting materials and modifications.
[00110] The bromoalkyl products made using the procedures set forth in this example are employed as starting materials for the syntheses set forth in Example 4.
[00111] Example 4. Synthesis of methyl 7-(cyclopropanecarbonyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Intermediate 4):
[00113] Methyl but-2-ynoate 3; (300 mg) was added to a solution of 2-bromo-l- cyclopropylethanone (1; 500 mg, 3.06 mmol) in 2-butyloxirane (5 mL) at room temperature. Pyridazine (2; 250 mg, 3.061mmol) was then added slowly. The reaction mixture was refluxed at 60 °C overnight. The solvent was removed in vacuo to afford the crude product. The crude product was purified by silica gel chromatography (petroleum ether /ethyl acetate =1: 1) to afford the title compound (120 mg , 9.6%).
[00114] The intermediates shown in the following table were prepared according to the procedure described in this example using the appropriate starting materials and modifications.
0 > pyr azne- -car oxyate
Intermediate Structure Name LCMS methyl 6-methyl-7-
41 nicotinoylpyrrolo[ 1 ,2- 296 b]pyridazine-5-carboxylate
0
[00115] Example 5. Synthesis of ethyl 6-methyl-7-(l-methyl-2-oxo-l,2- dihvdropyridine-4-carbonyl)pyrrolori,2-b1pyridazine-5-carboxylate (Intermediate 4.T).
A mixture of tributyl(l-ethoxyvinyl)tin (11.41 g, 31.59 mmol), 4-bromo-l-methylpyridin- 2(lH)-one (5.4 g, 28.72 mmol), bis(triphenylphosphine)palladium(II) chloride (201.59 mg, 287.20 μιηοΐ), and toluene (50 mL) was heated under nitrogen at 100 °C for 20 hours. After hydrolysis of the reaction mixture with 5% hydrochloric acid, the organic layer was extracted with acetic ether (30 mLx3), and dried over sodium sulfate. After evaporation of the solvent, column chromatography of the residue(silica gel; 100% Ethyl acetate) provided the title compound (3.5 g, yield: 80.62%).
[00117] Step 2: 4-(2-bromoacetyl)-l-methylpyridin-2(lH)-one:
To a solution of 4-acetyl-l-methylpyridin-2(lH)-one (3.5 g, 23.15 mmol) in ethyl acetate (20 mL) and chloroform (20 mL) was added copper(II) bromide (10.34 g, 46.31 mmol). The mixture was stirred at 70 °C for 12 hours. The mixture was filtered, the filtrate was evaporated and purified via silica gel chromatography (80% PE/EA) to afford the title compound (1.5 g, yield, 28%).
[00118] Step 3: ethyl 6-methyl-7-(l-methyl-2-oxo-l,2-dihydropyridine-4- carbon l)pyrrolo[l,2-b]pyridazine-5-carboxylate:
[00119] A mixture of 4-(2-bromoacetyl)-l-methylpyridin-2(lH)-one (1.5 g, 6.52 mmol), ethyl but-2-ynoate (804 mg, 7.17 mmol), pyridazine (522 mg, 6.52 mmol), and 2- ethyloxirane (20 mL) was heated under nitrogen at 60 °C for 12 h. The mixture was evaporated and purified via silica gel chromatography (5% MeOH/DCM) to afford the title compound (500 mg, yield, 22.6%).
[00120] Example 6. Synthesis of ethyl 6-(l-((benzyloxy)carbonyl)piperidine-4- carbonyl)-7-methylpyrrolo[l,2-a]pyrazine-8-carboxylate (Intermediate 4K):
To a solution of benzyl 4-(2-bromoacetyl)piperidine-l-carboxylate (8 g, 23.5mmol) in (methylsulfinyl)methane (40 mL) were added ethyl but-2-ynoate (3.1 g, 28.2mmol), pyrazine (2.2 g, 28.2 mmol) and potassium carbonate (9.7g, 70.5 mmol). The reaction mixture was stirred at room temperature overnight. The reaction was then quenched by adding water (100 mL). The mixture was extracted with ethyl acetate. The organic layer was dried over sodium sulfate and concentrated to give the crude product and purified by column chromatography on silica gel (eluted: petrol ether / acetic ester 20: 1→2: 1) to afford the title compound (770 mg, 7.3%) as a yellow oil. m/z: calc'd 449.2; found 449.9
[00121] The intermediates shown in the following table were prepared according to the procedure described in this Example using the appropriate starting materials and modifications.
[00122] Example 7. ethyl 8-(l-(tert-butoxycarbonyl)piperidine-4-carbonyl)-7- methylpyrrolo[l,2-a1pyrazine-6-carboxylate (Intermediate 4M):
To a solution of l-(2-ethoxy-2-oxoethyl)pyrazin-l-ium bromide (11.9 g, 48 mmol) in (methylsulfinyl)methane (20 mL) was added tert-butyl 4-(but-2-ynoyl)piperidine-l- carboxylate (6 g, 23.9 mmol), and potassium carbonate (6.6 g, 48 mmol) followed. The resulting reaction mixture was allowed to stir for 2 hours at room temperature. The reaction mixture was washed with water and brine, extracted with acetic ester(100 mL x 3). The combined organic phase was dried by anhydrous sodium sulphate, and then filtered. The filtrate was concentrated and purified by column chromatograph on silica gel (eluted: petrol ether / acetic ester 10: 1→3: 1→1: 1) to afford the title compound (3 g, 31 %) as an orange oil. LCMS (M + H+) m/z: calc'd. 415.21, found 415.9.
[00123] Example 8. Synthesis of methyl 7-(l-cyclopropyl-l-hvdroxyethyl)-6- methylpyrrolori,2-b1pyridazine-5-carboxylate (Intermediate 6):
[00124] Step 1: methyl 7-(l-cyclopropyl-l-hydroxyethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylate (Intermediate 5):
To a solution of 7-(cyclopropylmethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (4; 110 mg, 0.43 mmol) in THF (1.5 mL) was added slowly a solution of methylmagnesium bromide (1.5 mL, 3 M in THF). The reaction mixture was stirred at -78 °C for 2 h. The mixture was then treated with sat'd aqueous NH4C1 (8 mL), and extracted with EtOAc (3 x 10 mL). The combined organic extract was dried (Na2S04), filtered, and cone, in vacuo to afford the title compound 5 which was used without further purification (43 mg, 36% ).
[00125] The intermediates shown in the following table were prepared according to the procedure described in Step 1 of this Example using the appropriate starting materials and modifications.
8 \¾ a]pyrazine-6-carboxylate
[00126] Step 2: methyl 7-(l-cyclopropyl-l-hydroxyethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylate (Intermediate 6):
7-(Cyclopropylmethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (5; 40 mg, 20 mg, 0.155 mmol) and Et3SiH (0.171 mmol) were dissolved in THF (1.5 mL). Trifhioroacetic acid (1.55 mL) was then added to the reaction and the resulting mixture was stirred at room temperature overnight. The solvent was then removed in vacuo to afford the crude product which was purified via column chromatography on silica gel (petroleum ether /ethyl acetate =5: 1) to afford the title compound 6 (18 mg, 45 %).
[00127] The examples shown in the following table were prepared according to the procedure described in Step 2 of this Example using the appropriate starting materials and modifications.
a]pyrazine-6-carboxylate
[00128] Example 9. Synthesis of ethyl 6-methyl-7-(l-(N- methylmethylsulfonamido)ethyl)pyrrolo[l,2-b1pyridazine-5-carboxylate (Intermediate 8J:
[00129] Step 1: ethyl 6-methyl-7-(l-(methylamino)ethyl)pyrrolo[l,2-b]pyridazine-5- carboxylate:
To a solution of ethyl 7-acetyl-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (4G; 0.33 g, 1.34 mmol) in MeOH (50 mL) was added methylamine (30 % in methanol, 0.42 g, 4.02 mmol) at room temperature. The mixture was stirred for one hour at room temperature before the addition of NaBH3(CN) (0.18 g, 2.68 mmol). The reaction was then allowed to stir at ambient temperature for 12 h before the addition of 1 N HC1 to adjust the pH ~3. The reaction was then extracted with MTBE. The remaining aqueous layer was then basified with 2N NaOH to pH -11 and that was extracted with EtOAc. The organic extract was dried over Na2S04 and concentrated in vacuo to afford the title compound as a pale yellow oil which was used without further purification (0.23 g, yield, 66 %) m/z 261.
[00130] The intermediates shown in the following table were prepared according to the procedure described in Step 1 of this Example using the appropriate starting materials and modifications.
[00131] Step 2: ethyl 6-methyl-7-(l-(N-methylmethylsulfonamido)ethyl)pyrrolo[l,2- b ]pyridazin -5-carboxylate:
[00132] To a cooled (0 °C) and stirred solution of ethyl 6-methyl-7-(l- (methylamino)ethyl)-4a,7-dihydropyrrolo[l,2-b]pyridazine-5-carboxylate (0.22 g, 0.84 mmol) and triethylamine (0.42 g, 4.18 mmol) in DCM (5 mL) was added Ms-Cl (0.28 g, 2.51 mmol). The mixture was then allowed to stir overnight to room temperature. The reaction mixture was then cone, in vacuo and the resulting residue was purified via silica gel chromatography (eluent: PE/EtOAc=10: l) to the title compound as a yellow oil (0.27 g, yield 93 %).
[00133] The intermediates shown in the following table were prepared according to the procedure described in Step 2 of this Example using the appropriate starting materials and modifications.
a]pyrazine-6-carboxylate
[00134] Example 10. Synthesis of ethyl 6-methyl-7-(l-(l-methyl-2-oxo-l,2- dihvdropyridin-3-yl)ethyl)pyrrolo[l,2-b1pyridazine-5-carboxylate (Intermediate 11).
[00135] Step 1: ethyl 6-methyl-7-(l-methyl-2-oxo-l,2-dihydropyridine-3- carbonyl)pyrrolo l,2-b]pyridazine-5-carboxylate (9):
To a solution of ethyl 6-methyl-7-(2-oxo-l,2-dihydropyridine-3-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylate ethyl (4D; 75 mg, 0.23 mmol) and potassium carbonate (63 mg, 0.46 mmol) in anhydrous MeCN (2 mL) was added methyl iodide (49 mg, 0.35 mmol). The reaction was warmed to 50 °C with stirring for 2 h before being allowed to cool to rt. The reaction mixture was then filtered and the filtrate was cone, in vacuo to afford the title compound which was used directly in the next step without purification.
[00136] The intermediate shown in the following table were prepared according to the procedure described in Step 1 of this Example using the appropriate starting materials and modifications.
[00137] Step 2: ethyl 6-methyl-7-(l-(l-methyl-2-oxo-l,2-dihydropyridin-3- yl)vinyl)pyrrolo[ -b ]pyridazine-5-carboxylate (10):
[00138] To a solution of ethyl 6-methyl-7-(l-methyl-2-oxo- l,2-dihydropyridine-3- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (250 mg, 0.74 mmol) in anhydrous tetrahydrofuran (10 mL) was added methylmagnesium bromide (1.48 mL, 1.48 mmol) at 0 °C. The reaction mixture was stirred at 0 °C to room temperature overnight before being quenched with 1 M HC1 and partitioned with water and ethyl acetate. The organic layer was dried and concentrated to afford the crude title compound as a yellow oil, which was used directly in the next step. (200 mg, 80% yield)
[00139] Step 3: ethyl 6-methyl-7-(l-(l-methyl-2-oxo-l,2-dihydropyridin-3- yl)ethyl)pyrrolo[ -b ]pyridazine-5-carboxylate ( 11 ):
A mixture of ethyl 6-methyl-7-(l-(l-methyl-2-oxo- l,2-dihydropyridin-3- yl)vinyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (200 mg, 0.74 mmol) and Pd/C (50 mg, 10%) in methanol (10 mL) was placed under an atmosphere of hydrogen and the reaction was stirred at room temperature overnight. The reaction was then purged, placed under nitrogen and was filtered and concentrated to afford the title compound which was used without further purification. (140 mg, 70%)
[00140] The intermediates shown in the following table were prepared according to the procedure described in this example using the appropriate starting materials and modifications.
[00141] Example 11. Synthesis of ethyl 6-methyl-7-(l-(l-methylpiperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (Intermediate 12):
A mixture of compound ethyl 6-methyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine- 5-carboxylate (360 mg, 1.14 mmol) in tetrahydrofuran (10 mL), were added formaldehyde (37%) aqueous solution (278 mg, 3.42 mmol) and sodium triacetoxyhydroborate (567 mg, 3.42mmol). The reaction mixture was stirred at 50 °C for 1 hour. The reaction mixture was quenched by the addition of saturated aqueous potassium carbonate. The aqueous layer was extracted with ethyl acetate (30 mL x 3). The combined organic layers were dried over sodium sulfate and concentrated to afford the title compound (376 mg, 100%) as a yellow oil. LCMS (M+H+) mJ . calc'd 329.21; found 329.9
[00142] Example 12. Synthesis of (+)-6-methyl-7-(l-phenylethyl)pyrrolori.2- blpyridazine-5-carboxylic acid (Intermediate 13):
Lithium hydroxide monohydrate (41 mg, 0.97 mmol) was added to ethyl 7-acetyl-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (40 mg, 0.16 mmol) in THF/H20 (10 mL, 1: 1) and the resulting mixture was heated to reflux with stirring overnight. The mixture was then allowed to cool to rt and was cone, in vacuo, diluted with water (5 mL), and acidified with IN HCl to a pH ~2. A precipitate formed which was collected via vacuum filtration to afford the title compound as a white solid (30 mg, yield 85 ).
[00143] The intermediates shown in the following table were prepared according to the procedure described above using the appropriate starting materials and modifications.
8 N a]pyrazine-6-carboxylic acid
[00144] Example U. Synthesis of 6-methyl-7-(l-(N- methylacetamido)ethyl)pyrrolo[l,2-b1pyridazine-5-carboxylic acid (Intermediate 14):
To the solution of ethyl 6-methyl-7-(l-(methylamino)ethyl)pyrrolo[l,2-b]pyridazine-5- carboxylate (70 mg, 0.26 mmol) in THF/MeOH/H20 (1: 1: 1, 2 mL) was added lithium hydroxide monohydrate (80 mg, 1.9 mmol). The mixture was then stirred with heating at reflux for 2 h. Then the reaction was then allowed to cool to rt and Ac20 was added (270 mg, 2.68 mmol). The reaction was then stirred overnight at room temperature and was cone, in vacuo. The resulting residue was diluted with water (5 mL), acidified with 1 N HC1 to pH ~2, and extracted with DCM (20 mL). The organic extract was dried over Na2S04, filtered and
concentrated to afford the title compound as a white solid which was used without further purification (40 mg, yield 54 %) m/z 311.
[00145] Example 14. Synthesis of 3-(aminomethyl)-4-methoxy-6-methylpyridin-2-ol (Intermediate 15):
Malononitrile (110 g, 1.67 mol) was dissolved in dry THF (1000 mL) and cooled in ice-water bath. NaH (60 % in mineral oil, 67 g, 1.67 mol) was added portionwise below 10°C very carefully while the reaction flash was evacuated with N2 flow. After addition completed, the mixture was stirred at 0°C for 30 min. Then 4-methyleneoxetan-2-one (140 g, 1.67 mol) was added dropwise below 0°C. After addition completed, the mixture was stirred at -10°C for 1 h. The reaction mixture was neutralized by 4 N HCl and concentrated under vacuum to afford the title compound an orange oil. The crude product was used to next step without further purification.
2-amino-6-methyl-4-oxo-4H-pyran-3-carbonitrile from above was dissolved in 4 N HC1/H20 (2500 mL) and refluxed for 5 h with stirring strongly. After cooled to r.t, the precipitate was filtered, washed with H20 (500 mL), ethanol (500 mL) and MTBE (200 mL) and dried under high vacuum to afford the title compound as a yellow powder. (165 g, yield 66 %)
2,4-dihydroxy-6-methylnicotinonitrile (40 g, 266.4 mmol) was dissolved in POCl3 (120 mL) and added by DMF (4 drops). The mixture was heated for 3 h. Then the mixture was concentrated under vacuum. The residue was dissolved in EtOAc (2 L) and neutralized by saturated NaHC03. Then the mixture was filtered through a Celite pad to remove the dark flocculating. The organic layer was separated, dried over Na2S04 and concentrated under vacuum to give the title compound as an off-white solid. (45 g, yield 90 %).
[00149] Step 4: 2, -dimethoxy-6-methylnicotinonitrile:
2,4-dichloro-6-methylnicotinonitrile (45 g, 240 mmol) was dissolved in CH3OH (300 mL). NaOMe (30 % in MeOH, 100 mL, 1680 mmol) was added. The mixture was refluxed for 4 h. After cooled to r.t, the reaction mixture was neutralized by HOAc. The solvent was removed under vacuum and the residue was washed with H20 (300 mL) and MTBE (100 mL). The resulting solid was coevaporated with dry THF (300 mL) to give the title compound as a dark- yellow solid. (40 g, yield 95 %)
2,4-dimethoxy-6-methylnicotinonitrile (10.0 g, 56 mmol) was dissolved in the mixture of THF (260 mL) and methanol (260 mL). Raney Ni (wet, 10.0 g), TEA (29.0 g, 280 mmol) and Boc20 (36.8 g, 168 mmol) were added. Then the mixture was hydrogenated (1 atom) at r.t. overnight. After reaction completed, the reaction mixture was filtered through a Celite pad. 6 parallel reactions were combined and concentrated under vacuum to give the title compound as a yellow solid. (84 g, 88 %)
[00151] Ste -(aminomethyl)-4-methoxy-6-methylpyridin-2-ol (15):
Tert-butyl ((2,4-dimethoxy-6-methylpyridin-3-yl)methyl)carbamate (83 g, 294 mmol) was dissolved in 4 N HC1/H20 (830 mL). Then the mixture was refluxed for 4.5 h. (The reaction mixture was monitored by MS spectrum to make sure the methyl group at 2-position de- protect completely.) After the reaction completed, the mixture was concentrated under vacuum to give a brown oil. The oil was suspended in EtOH (300 mL) for 15 min to give a yellow precipitate. The precipitate was filtered, washed with ethanol (100 mL) and MTBE (100 mL) and dried under high vacuum to give 38 g of fraction 1 3-(aminomethyl)-4- methoxy-6-methylpyridin-2-ol (Purity 98 % by LCMS, yield 63 %) as a yellow powder. In the meantime, the filtration from fraction 1 was concentrated under vacuum and the residue
was solidified by ethanol (100 mL). The precipitate was filtered, washed with ethanol (100 mL) and MTBE (100 mL) and dried under high vacuum to give 20 g of the title compound as a yellow powder.
[00152] Example 15. Synthesis of 3-(aminomethyl)-4,6-dimethylpyridin-2(lH)-one (Intermediate 16).
To a solution of pentane-2,4-dione (100 g, 1.0 mol) in H20 (2 L) were added 2- cyanoacetamide (84 g, 1.0 mol) and K2C03(13.8 g, 0.1 mol). Then the mixture was stirred at room temperature for 16 hr. The reaction solution was filtrated to give crude product. The crude was washed with water and concentrated to give the title compound (138 g, 93 %).
[00154] Step 2: tert-butyl ((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)carbamate:
To a solution of 4,6-dimethyl-2-oxo-l,2-dihydropyridine-3-carbonitrile (40 g, 0.27 mol) in THF/CH3OH (1: 1, 2 L) were added Ni (40 g), Boc2O(110 g, 0.5 mol) and Et3N(50 g, 0.5 mol). Then the mixture was stirred in H2 atmosphere at room temperature for 48 hr. The reaction solution was filtrated and concentrated to give crude product. The crude was added H20 (200 mL) and extracted by DCM (600 mL x 3). The organic layer was concentrated to give the title compound (40 g, 56 %) for next step.
[00155] Step 3: 3-(aminomethyl)-4,6-dimethylpyridin-2(lH)-one (16):
Tert-butyl ((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)carbamate (40 g, 0.27 mol)was added into dioxane/HCl (1 L)and the mixture was stirred at room temperature for 4 hr. The reaction solution was filtrated and concentrated to give crude product. The crude was washed with ethyl acetate (lOOmL x 2) and EtOH (50mL x 1) and concentrated to afford the title compound as its HCI salt (15 g, 40 %). LCMS (M + H+) m/z: calc'd. 152.19; found
153.1. 1H NMR (DMSO, 400MHz) δ 11.84 (s, 1H), 8.07 (s, 3H), 5.96 (s, 1H), 3.76-7.75(d, J = 5.6 Hz, 2H), 2.21 (s, 3H), 2.15 (s, 3H).
[00156] The intermediate shown in the following table were prepared according to the procedure described in Steps 1-3 of this Example using the appropriate starting materials and modifications.
[00157] Example 16. Synthesis of 7-acetyl-N-((4-methoxy-6-methyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b1pyridazine-5-carboxamide
To a solution of 7-acetyl-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (13; 40 mg, 0.18 mmol) in dichloromethane (10 mL) was added with lH-benzo[d][l,2,3]triazol-l-ol (15; 140 mg, 0.37 mmol) and triethylamine (56 mg, 0.55 mmol). The reaction was allowed to stir for 30 minutes and 3-(aminomethyl)-4-methoxy-6-methylpyridin-2(lH)-one (40 mg, 0.24 mmol) was added and the reaction mixture was stirred at room temperature for 3 h. The solution was then cone, in vacuo, diluted with water (20 mL), and extracted with ethyl acetate (20 mL). The combined organic phase was dried over anhydrous sodium sulfate, filtered and concentrated to afford a residue. The residue was purified by preparative-HPLC (Column: YMC C18 150*30mm*5um; Mobile phase A: water with 0.1 % HC1; Mobile phase B: MeCN; column temperature: 30 °C; Gradient: B/A 35-65%) to afford the title compound as yellow solid (10 mg, yield 14 %). LCMS (M + H+) m/z: calc'd 368.15; found 369.2. 1H NMR
(400 MHz, CD3OD) δ 8.46-8.44 (m, IH), 8.30-8.27 (d, IH), 7.17-7.13 (dd, IH), 6.94 (s, IH), 4.11 (s, 3H), 2.79 (s, 3H), 2.64(s, 3H), 2.54 (s, 3H).
[00158] The compounds of the invention shown in the following table were prepared according to the procedure described above using the indicated starting materials and art- known modifications.
N-((4-methoxy-6-methyl-2- (d, J=7.53 Hz, 3 H) 2.33 (s, 3 H)
oxo- 1 ,2-dihydropyridin-3- 2.41 (s, 3 H) 3.49 (s, 3 H) 3.95 (s, 3
yl)methyl)-6-methyl-7-( 1 -( 1 - H) 4.51 (br. s., 2 H) 5.34 (t, J=5.02
110 methyl-2-oxo- 1 ,2- Hz, 1 H) 6.14 - 6.20 (m, 1 H) 6.30 462 13M, 15 dihydropyridin-4- (br. s., 1 H) 6.47 (s, 1 H) 6.75 (d,
yl)ethyl)pyrrolo[ 1 ,2- J=5.52 Hz, 1 H) 7.45 (d, J=7.03 Hz,
b]pyridazine-5-carboxamide 1 H) 8.09 (d, J=4.52 Hz, 1 H) 8.19
(d, J=10.54 Hz, 1 H)
(+)-N-((4-methoxy-6-methyl- (400 MHz, CD3OD): 8.15-8.17
2-oxo- 1 ,2-dihydropyridin-3- (m, 2H), 7.7-7.71(m, 7H), 7.48-7.50
yl)methyl)-6-methyl-7-( 1 -( 1 - (m, IH), 6.78-6.83 (m, 2H), 4.95-
111 methyl-2-oxo- 1 ,2- 5.02 (m, IH), 4.55 (s, 2H), 4.09 (s, 462 131, 15 dihydropyridin- 3 - 3H), 3.47 (s, 3H), 2.5 (s, 3H), 2.45
yl)ethyl)pyrrolo[ 1 ,2- (s, 3H), 1.69-1.70 (d, J = 7.2 Hz,
b]pyridazine-5-carboxamide 3H)
(+)-N-((4-methoxy-6-methyl-
(CDCI3, 400 M Hz) δ 8.54-8.52 (d,
2-oxo- 1 ,2-dihydropyridin-3- IH), 8.03-8.02 (d, IH), 6.69-6.65(q,
yl)methyl)-6-methyl-7-(l-
112 IH), 5.88 (s IH), 5.05-5.03 (q, IH), 448 130, 15 (methylsulfonamido)ethyl)py
4.54 (s, 2H), 3.83(s, 3H), 2.47(s,
rrolo [ 1 ,2-b] pyridazine- 5 - 3H), 2.25(s, 6H), 1.61-1.59 (d, 3H)
carboxamide
(400 MHz, CDCI3) 3.88 (s,
(+)-N-((4-methoxy-6-methyl- 3H)12.83 (s, IH), 4.61-4.63 (d, J =
2-oxo- 1 ,2-dihydropyridin-3- 5.6 Hz, 2H), 4.99-5.02 (d, J = 7.6
yl)methyl)-6-methyl-7-(l- Hz, IH), 5.92 (s, IH), 6.57-6.61 (m,
113 432 13J, 15
(pyridin-3- IH), 7.12-7.16 (m, IH), 7.26-7.40 yl)ethyl)pyrrolo[ 1 ,2- (m, IH), 7.52-7.56 (m, IH), 7.97- b]pyridazine-5-carboxamide 8.00 (m, IH) , 8.38-8.52 (m, 2H) ,
8.52 (s, IH), 12.83 (s, IH)
7-(3,5-dimethylisoxazol-4- (400 MHz, CDCI3) δ: 2.09 (s, 3H),
yl)-N-((4-methoxy-6-methyl- 2.24-2.40 (m, 9H), 3.91 (s, 3H),
2-oxo- 1 ,2-dihydropyridin-3- 4.65-4.67 (d, / = 5.6 Hz, 2H), 5.96
114 421 13E, 15 yl)methyl)-6- (s, IH), 6.72-6.76 (m, IH) , 7.49- methylpyrrolo [1,2- 7.53 (m, IH) , 8.05-8.08 (m, IH) ,
b]pyridazine-5-carboxamide 8.63-8.67 (m, IH) , 12.32 (s, IH)
(400 MHz, Methanol-d4) 1.01-0.99
(m, IH), 1.23-1.13 (m, 2H), 1.44
(+)-N-((4-methoxy-6-methyl- (d, J=7.2 Hz, 3H), 2.03-2.00 (m,
2-oxo- 1 ,2-dihydropyridin-3- IH), 2.18-2.15 (m, IH), 2.34 (s, 3H),
yl)methyl)-7-methyl-6-(l- 2.46 (s, 3H), 3.18-3.14 (m, IH),
115 439 13R, 15
(tetrahydro-2H-pyran-4- 3.28-3.25 (m, IH), 3.50-3.44 (m,
yl)ethyl)pyrrolo[ 1 ,2- IH), 3.80-3.76 (m, IH), 3.97 (s, 3H),
a]pyrazine-8-carboxamide 4.05-4.01 (m, IH), 4.54 (s, 2H), 6.30
(s, IH), 7.51-7.50 (m, 2H), 8.22-8.20
(m, IH), 8.98 (s, IH)
b]pyridazine-5-carboxamide 1H) 8.72-8.70 (m, 1H) 12.04 (s, 1H)
(400 MHz,Methanol-d4) 1.11-1.12
(m, 2H), 1.32-1.37(m, 3H), 1.42
(±)-7-(l-(l- (d, J=7.2Hz, 2H) 2.10 (d, J=13.2Hz,
(ethylsulfonyl)piperidin-4- lH), 2.21(m, 1H), 2.31(s, 3H),
yl)ethyl)-N-((4-methoxy-6- 2.47(s,3H), 2.50-2.57(m, 1H), 2.75-
121 methyl-2-oxo- 1 ,2- 530 13L, 15
2.88(m, 4H), 3.60 (d, J=12 Hz, 2H),
dihydropyridin- 3 - yl)methyl) - 3.7-4.0 (m, 4H), 4.64-4.67 (m, 2H),
6-methylpyrrolo[ 1 ,2-
5.97 (s, 1H), 6.56-6.61 (m,
b]pyridazine-5-carboxamide
1H) C7.40-7.43(m, 1H), 8.00-8.02
(m, 1H), 8.46 (d, J=8.6 Hz, 1H)
(400 MHz, Methanol-d4) 2.02 (s,
7-( 1 ,4-dimethyl- lH-pyrazol- 3H) 2.47 (s, 3H) 2.58 (s, 3H) 4.12 (s,
3-yl)-N-((4-methoxy-6- 3H) 4.16 (s, 3H) 4.63 (s, 2H) 7.03 (s,
methyl-2-oxo- 1 ,2-
122 1H) 7.09-7.07 (m, 1H) 8.02 (s, 1H) 421 13G, 15 dihydropyridin- 3 - yl)methyl) - 8.17-8.14 (m, 0.5H) 8.31-8.30 (m,
6-methylpyrrolo[ 1 ,2- 1H) 8.39-8.37 (m, 1H) 8.92-8.69 (m,
b]pyridazine-5-carboxamide
0.5 H)
(400 MHz, CDC13) <5: 1.01 (t, J=7.2
7-(3,5-dimethylisoxazol-4- Hz, 3H), 1.62-1.66 (m, 2H), 2.08 (s,
yl)-6-methyl-N-((6-methyl-2- 3H), 2.45 (s, 6H), 2.37 (s, 3H), 2.73
oxo-4-propyl-l,2- (t, J=7.6 Hz, 2H), 4.60 (d, J=4 Hz, 13E,
123 434
dihydropyridin- 3 - 2H), 5.95 (s, 1H), 6.74 (q, J=4.4 Hz, 16B yl)methyl)pyrrolo[ 1 ,2- 1H), 7.44-7.47 (brs, 1H), 8.06
b]pyridazine-5-carboxamide (dd,J=4 Hz, J=1.6 Hz, 1H), 8.59
(d, J=8 Hz, 1H),11.11 (brs, 1H)
(400 MHz, MeOD) δ 1.89-1.96 (m,
(+)-N-((4,6-dimethyl-2-oxo- 3H), 2.20-2.16(d, J=14.4 Hz, 3H),
1 ,2-dihydropyridin-3- 2.37(s, 3H), 2.50(s, 3H), 2.62(s, 3H), yl)methyl)-6-methyl-7-( 1 -( 1 - 2.65(s, 2H), 2.87(m, 3H), 3.17 (m,
124 436 13P, 16 methylpiperidin-4- 2H), 3.60 (d, J=12 Hz, 2H), 4.13- yl)ethyl)pyrrolo[ 1 ,2- 4.19 (m, 1H), 4.57 (s, 2H), 6.58 (s, b]pyridazine-5-carboxamide lH), 7.15(m, 1H), 8.29-8.32 (d,
J=12 Hz, 1H), 8.46 (m, 1H)
[00159] Example 17. Synthesis of (R or S)-7-(l-(N-ethylmethylsulfonamido)ethyl)-N- ((4-methoxy-6-methyl-2-oxo-l,2-dihvdropyridin-3-yl)methyl)-6-methylpyrrolori,2- blpyridazine-5-carboxamide (Peak 1; Compound 107) and (R or S)-7-(l-(N- ethylmethylsulfonamido)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihvdropyridin-3- yl)methyl)-6-methylpyrrolori,2-b1pyridazine-5-carboxamide (Peak 2; Compound 108):
Peak 1 - Compund 107 Peak 2- Compound 108
To a suspension of 7-(l-(N-ethylmethylsulfonamido)ethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylic acid (13M; 130 mg, 0.4 mmol) in DMF (6 mL) was added TEA (202 mg, 2.0 mmol), 3-(aminomethyl)-4-methoxy-6-methylpyridin-2(lH)-one hydrochloride (15; 106 mg, 0.52 mmol) and HATU (303 mg, 0.8 mmol) at 25 °C under N2. The reaction was stirred for 4 h before being diluted with EtOAc and washed with sat'd aqueous NaHC03. The organic layer was concentrated under vacuum and purified by preparatory HPLC (Column: Gemini 150*25mm Mobile phase A: water with 0.1% NH3.H20 solution Mobile phase B: MeCN 20-50-15) to give pure racemic title compound (20 mg, yield 10.5 %)
[00160] The racemic mixture (18 mg) was separated via SFC (Column: AD (250*30mm, 5um); Flow rate: 50mL/min: Mobile: A, phase: 40% IPA+NH3.H20, B, 60% C02; Wavelength: 220 nm) to give peak 1 (Compound 107; 3.9 mg) and peak 2 (Compound 108; 6.5 mg).
[00161] Peak 1: LCMS (M + H+) mlz: calc'd 475.19; found 475.9. 1H NMR (CDC13, 400 M Hz) d 11.79 (s, 1H), 8.53 (dd, Jl = 1.6 Hz, J2 = 8.8 Hz, 1H), 8.10 (dd, /,= 1.6 Hz, J2 = 4.4 Hz, 1H), 7.45 (d, J = 4.8 Hz, 1H), 6.71 (dd, J, = 4.4 Hz, J2 = 9.2 Hz, 1H), 5.96 (s, 1H), 5.70 (dd, J, = 7.2 Hz, J2 = 14.8 Hz, 1H), 4.63 (d, J = 5.6 Hz, 2H), 3.91 (s, 3H), 3.67-3.48 (m, 1H), 3.37-3.30 (m, 1H), 2.62 (d, J = 14.8 Hz, 6H), 2.31 (s, 3H), 1.87 (d, J = 7.6 Hz, 3H), 0.95 (t, J = 7.2 Hz, 3H).
[00162] Peak 2: LCMS (M + H+) mlz: calc'd 475.19; found 476.0. 1H NMR (CDC13, 400 M Hz) d 11.33 (s, 1H), 8.53 (dd, J, = 1.6 Hz, J2 = 8.8 Hz, 1H), 8.10 (dd, /,= 1.6 Hz, J2 = 4.4 Hz, 1H), 7.45 (d, J = 4.8 Hz, 1H), 6.71 (dd, Jl = 4.4 Hz, J2 = 9.2 Hz, 1H), 5.96 (s, 1H), 5.70 (dd, J, = 7.2 Hz, J2 = 14.8 Hz, 1H), 4.63 (d, J = 5.6 Hz, 2H), 3.91 (s, 3H), 3.67-3.48 (m, 1H),
3.37-3.30 (m, 1H), 2.62 (d, J = 14.8 Hz, 6H), 2.31 (s, 3H), 1.87 (d, J = 7.6 Hz, 3H), 0.95 (t, J = 7.2 Hz, 3H).
[00163] Compound 101 was separated into its isolated enantiomers according to the procedure described in this Example. The separated enantiomers were characterized as below:
[00164] Example 18. Synthesis of 7-(l-(l-(2,2-difluoropropanoyl)piperidin-4- yl)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihvdropyridin-3-yl)methyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 135).
[00165] Step 1: Synthesis of 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[ 1,2-b ]pyridazine-5-carboxylic acid.
[00166] To a solution of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (6D; 450 mg, 1.0 mmol ) in MeOH (3.0 mL), THF (3.0 mL), and water (2.0 mL) was added lithium hydroxide hydrate (420 mg, 10.0 mmol ) and the reactions was stirred at reflux for 24 hours. The reaction solution was concentrated and the residue was adjusted pH to 3-4 with 1.0 M HC1. The solution was then extracted with EtOAc and the combined organics phase was washed with brine, dried over Na2S04, filtered, and concentrated to afford 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (390 mg, 92% yield). LCMS (M + H+) mlz 422.
[00167] Step 2: Synthesis of benzyl 4-(l-(5-(((4-methoxy-6-methyl-2- dihydropyridin-3-yl)methyl)carbamoyl)-6-methylpyrrolo[l,2-b]pyridazin-7- yl)ethyl)piperidine-l-carboxylate.
[00168] To a solution of 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (390 mg, 0.925 mmol ) in dichloromethane (10.0 mL) was added 3-(aminomethyl)-4-methoxy-6-methylpyridin-2(lH)-one hydrochloride (284 mg, 1.39 mmol), (l-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino- morpholino-carbenium hexafluorophosphate (475 mg, 1.11 mmol)(COMU), and triethylamine (468 mg, 4.63 mmol). The reaction was stirred at 25 °C for 17 h before the reaction mixture was filtered. The filtrate was concentrated and the crude residue was purified by silica gel chromatography (eluent: dichloromethane/MeOH=50/l ) to afford benzyl 4-(l-(5-(((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)carbamoyl)-6- methylpyrrolo[l,2-b]pyridazin-7-yl)ethyl)piperidine-l-carboxylate (500 mg, yield 94%). LCMS (M + H+) mlz 572.
[00169] Step 3: Synthesis of N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxam
[00170] To a solution of benzyl 4-(l-(5-(((4-methoxy-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)carbamoyl)-6-methylpyrrolo[l,2-b]pyridazin-7- yl)ethyl)piperidine-l-carboxylate (500 mg, 0.875 mmol ) in MeOH (10.0 mL) was added palladium on carbon (200 mg, 10 wt % Pd) and the reaction was stirred under hydrogen at 25 °C for 2.5 hours. The reaction mixture was then filtered and the filtrate was concentrated to give N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-6-methyl-7-(l- (piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (350 mg, yield 91%). LCMS (M + H+) mlz 438.
[00171] Step 4: Synthesis of 7-(l-(l-(2,2-difluoropropanoyl)piperidin-4-yl)ethyl)-N-
((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-6-methylpyrrolo[l,2- b Ipyridazin e-5- carb oxam ide (Compound 135).
[00172] To a solution of N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (80 mg, 0.183mmol ) in dichloromethane (3.0 mL) was added 2,2-difluoropropanoic acid (30.0 mg, 0.274 mmol ), (l-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino-morpholino- carbenium hexafluorophosphate (94 mg, 0.219 mmol) (COMU), and triethylamine (92.5 mg, 0.914 mmol). The reaction was stirred at room temperature for 3 h before the reaction mixture was concentrated. The crude residue was purified by preparative-HPLC (Condition: Column: ASB C18 150*25mm; Mobile phase A: MeCN; Mobile phase B: water with 0.1% HC1; column temperature: 30°C; Gradient: B in A, 34-64% ; Flow rate: 25ml/min) to afford 7-(l-(l-(2,2-difluoropropanoyl)piperidin-4-yl)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxamide (5.6 mg, 6% yield). LCMS (M + H+) m/z: 530. 1H NMR (400MHz, MeOH-d4) δ 8.22 - 8.15 (m, 2H), 6.83 - 6.76 (m, 2H), 4.55 (br. s., 2H), 4.33 (d, J=13.4 Hz, 1H), 4.24 (d, J=12.8 Hz, 1H), 4.07 (s, 3H), 3.19 - 3.11 (m, 1H), 2.99 - 2.88 (m, 1H), 2.80-2.74 (m, 1H), 2.55 (br. s., 1H), 2.48 (br. s., 3H), 2.42 (s, 3H), 2.16 (d, J=13.2 Hz, 1H), 1.83 - 1.69 (m, 3H), 1.43 (d, J=7.2 Hz, 3H), 1.29 - 1.21 (m, 1H), 1.06 (br. s., 1H), 1.01 - 0.91 (m, 1H)
[00173] The following compounds were synthesized following step 4 using the appropriate starting materials and modifications:
Name 1H NMR LCMS No.
1H NMR (400MHz, MeOH-d4) δ 8.18 (d,
J=7.6 Hz, 2H), 6.89 (s, 1H), 6.84 - 6.80 (m,
N-((4-methoxy-6-methyl-2- 1H), 4.56 (s, 2H), 4.31 (d, J=13.6 Hz, 1H), oxo- 1 ,2-dihydropyridin-3-
4.10 (s, 3H), 3.98 (d, J=13.0 Hz, 1H), 3.70 (d, yl)methyl)-6-methyl-7-(l-(l-
J=13.4 Hz, 1H), 3.48-3.44 (m, 1H), 3.38-3.35
137 (3,3,3- 548
(m, 1H), 3.18 - 3.09 (m, 1H), 2.98 - 2.86 (m, trifluoropropanoyl)piperidin-
1H), 2.73 - 2.62 (m, 1H), 2.52 (s, 3H), 2.43 (s,
4-yl)ethyl)pyrrolo[ 1 ,2- 4H), 2.13 (t, J=12.0 Hz, 1H), 1.44 (d, J=7.2 b]pyridazine-5-carboxamide
Hz, 3H), 1.06 - 1.00 (m, 1H), 0.99 - 0.88 (m,
1H)
1H NMR (400MHz, MeOH-d4) δ 8.19 (d,
J=7.2 Hz, 2H), 6.91 (s, 1H), 6.86 - 6.81 (m,
7-(l-(l-(2,2- 1H), 6.40 - 6.31 (m, 1H), 4.56 (s, 2H), 4.51 (d, difluoroacetyl)piperidin-4- J=13.6 Hz, 1H), 4.24 (d, J=13.2 Hz, 1H), 4.10 yl)ethyl)-N-((4-methoxy-6- (s, 3H), 3.79 (d, J=14.0 Hz, 1H), 3.21 - 3.12
138 methyl-2-oxo- 1 ,2- 516
(m, 1H), 3.00 - 2.89 (m, 1H), 2.78 (t, J=12.0 dihydropyridin- 3 - yl)methyl) - Hz, 1H), 2.53 (s, 3H), 2.46 - 2.41 (m, 3H),
6-methylpyrrolo[ 1 ,2- 2.17 (br. s., 1H), 1.44 (d, J=6.8 Hz, 3H), 1.31 - b]pyridazine-5-carboxamide
1.22 (m, 1H), 1.10 - 1.05 (m, 1H), 1.04 - 0.94
(m, 1H)
1H NMR (400MHz, MeOH-d4) δ 8.16 (d,
J=9.2 Hz, 1H), 8.09 (dd, J=1.2, 4.4Hz, 1H), 6.70 (dd, J=4.4, 8.8 Hz, 1H), 6.25 (s, 1H), 4.49
N-((4-methoxy-6-methyl-2-
(s, 2H), 4.25 (d, J=13.6 Hz, 1H), 4.06 (d, oxo- 1 ,2-dihydropyridin-3- J=14.4 Hz, 0.5H), 3.92 (s, 3H), 3.80 (d, J=13.2 yl)methyl)-6-methyl-7-(l-(l- Hz, 0.5H), 3.22 (d, J=12.0 Hz, 1H), 3.02 (t,
139 (2,2,2- 534
J=12.0 Hz, 0.5H), 2.87 (t, J=12.0 Hz, 0.5H), trifluoroacetyl)piperidin-4- 2.65 (t, J=12.0 Hz, 1H), 2.54 - 2.46 (m, 1H), yl)ethyl)pyrrolo[ 1 ,2-
2.44 - 2.37 (m, 3H), 2.30 (s, 3H), 2.19 (d, b]pyridazine-5-carboxamide
J=13.6 Hz, 1H), 1.43 (d, J=7.2 Hz, 3H), 1.31 - 1.23 (m, 1H), 1.13 (br. s., 1H), 1.07 - 0.97 (m,
1H)
[00174] Example 19. Synthesis of N-((4-methoxy-6-methyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-6-methyl-7-(l-(l-(3 rifluoropropyl)piperidin-4- yl)ethyl)pyrrolori,2-b1pyridazine-5-carboxamide (Compound 134).
[00175] Step 1: Synthesis of ethyl 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin- -yl)ethyl)pyrrolo[ 1,2-b ]pyridazine-5-carboxylate.
[00176] A mixture of ethyl 6-methyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (11A, 50 mg, 0.16mmol), 3-bromo-l,l,l-trifluoropropane (42
mg, 0.28mmol) and potassium carbonate (65 mg, 0.48 mmol) in acetonitrile (5 mL) was stirred at 50 °C for 5 hours under nitrogen. The mixture was diluted with EtOAc (50 ml) and water (50 ml). The layers were separated and the organic layer was dried over Na2S04, filtered, and concentrated to afford ethyl 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (50 mg yellow oil , 77 % yield). LCMS (M + H+) m/z 412.
[00177] Step 2: Synthesis of 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b ]pyridazine-5-carbox lic aci
[00178] A mixture of ethyl 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (50 mg, 0.122 mmol) and lithium hydroxide ( 29.1 mg, 1.22mmol ) dissolved in MeOH (2 mL), water (lmL), and THF (1 mL) was stirred at 60 °C for 16 hours under nitrogen. The reaction solution was acidified with aqueous hydrochloric acid (1M) to pH=5 and diluted with EtOAc (50 ml) and water (50 ml). The layers were separated and the organic layer was dried over Na2S04, filtered, and concentrated to afford 6-methyl-7-( l-( l-(3,3,3-trifluoropropyl)piperidin-4-yl)ethyl)pyrrolo[ 1 ,2- b]pyridazine-5-carboxylic acid (40 mg yellow solid , 86 % yield). LCMS (M + FT) m/z 384.
[00179] Step 3: Synthesis of N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(l-(l-(3,3,3 rifluoropropyl)piperidin-4-yl)ethyl)pyrrolo[l,2- b Ipyridazin e- 5-carboxam ide.
[00180] A mixture of 6-methyl-7-(l-(l-(3,3,3-trifluoropropyl)piperidin-4- yl)ethyl)pyrrolo[l,243]pyridazine-5-carboxylic acid (40 mg, 0.104mmol), 3-(aminomethyl)-4- methoxy-6-methylpyridin-2(lH)-one (22 mg, 0.125mmol), triethylamine (32 mg, 0.313 mmol), and (l-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino-morpholino-carbenium
hexafluorophosphate (54 mg, 0.125mmol) in DMF (3 mL) was stirred at 20 °C for 3 h under a nitrogen atmosphere. The mixture was washed with brine (5 mL), dried over Na2S04, filtered, and concentrated to give the crude product. The crude product was separated by preparative HPLC (Condition: Column: ASB CI 8 150*25mm; A: Water+0.1 HC1; B: CH3CN; column temperature:
30°C; Gradient: B in A 12-42%) to afford N-((4-methoxy-6-methyl-2-oxo-l ,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(l-(l-(3,3,3-trifluoropro^
carboxamide (15.4 mg, blue oil, 28% yield). LCMS (M + H+) m/z 534. lU NMR (400 MHz, MeOD) δ 8.23-8.17 (m, 2H), 6.85-6.89 (m, 2H) , 4.55 (s, 2H) ,4.07 (s, 3H), 3.71-3.64 (d, J = 24 Hz, 1H), 3.41- 3.36 (m, 1H), 3.11-3.10 (m, 2H), 2.79-2.75 (m, 3H) , 2.49 (s, 3H), 2.45 (s, 3H),2.45-2.35 (d, J = 36.8 Hz, 1H) , 1.59-1.56 (m, 2H), 1.47-1.45 (d, J = 7.2Hz, 3H), 1.39-1.36 (m, 1H), 1.32-1.26 (m, 2H).
[00181] Example 20. Synthesis of ethyl 7-(l-(l-(2-fluoro-2- methylpropanoyl)piperidin-4-yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Compound 144).
[00182] Step 1: Synthesis of ethyl 7-(l-(l-(2-fluoro-2-methylpropanoyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Intermediate 17 ) .
[00183] A mixture of ethyl 6-methyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (50 mg, 0.16mmol), 2-fluoro-2-methylpropanoic acid (20 mg, 0.19 mmol), 2-(3H-[l,2,3]triazolo[4,5-b]pyridin-3-yl)- l,l,3,3-tetramethylisouronium hexafluorophosphate(V) (72 mg, 0.19mmol) and triethylamine (48 mg, 0.48mmol) in dichloromethane (5 mL) was stirred at 20 °C for 3 h under nitrogen. The mixture was diluted with EtOAc (50 ml) and water (50 ml). The layers were separated and the organic layer was dried over Na2S04, filtered, and concentrated to afford crude material, which was purified by prep-TLC (petroleum ether: EtOAc =10: 1) to afford ethyl 7-(l-(l-(2-fluoro-2- methylpropanoyl)piperidin-4-yl)ethyl)-6-methylpyrrolo[ 1 ,2-b]pyridazine-5-carboxylate (50 mg yellow oil , 78% yield). LCMS (M + H+) m/z 404.
[00184] The following intermediates were synthesized in a similar manner to ethyl 7-
(l-(l-(2-fluoro-2-methylpropanoyl)piperidin-4-yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5- carboxylate using the appropriate starting materials and modifications:
[00185] Step 2: Synthesis of ethyl 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylat (Intermediate 18A).
[00186] To a solution of ethyl 7-(l-(l-(2-fluoro-2-methylpropanoyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (50 mg, 0.13mmol) in THF (3 mL) was added borane (2 mL, 10M/L) dropwise at 25°C. It was stirred at 25°C for 2 h before being quenched by addition of MeOH (5mL) at 0°C. The solution was diluted with EtOAc (50 ml) and water (50 ml). The layers were separated and the organic layer was dried over Na2S04, filtered, and concentrated to afford crude material which was purified by prep-TLC (petroleum ether: EtOAc =3: 1) to afford ethyl 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate (30 mg yellow oil , 62% yield). LCMS (M + H+) m/z 390.
[00187] The following intermediates were synthesized in a similar manner to ethyl 7-
(l-(l-(2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5- carboxylate using the appropriate starting materials and modifications:
a]pyrazine-8-carboxylate
[00188] Step 3: Synthesis of 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (Intermediate 19A).
[00189] A mixture of ethyl 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (30 mg, 0.07 mmol) and lithium hydroxide ( 19 mg, 0.77mmol ) in MeOH (2 mL) ,water (ImL) , and THF (1 mL) was stirred at 60 °C for 16 h under nitrogen. It was acidified with aqueous hydrochloric acid (1M) to pH=5 and diluted with EtOAc (50 ml) and water (50 ml). The layers were separated and the organic layer was dried over Na2S04, filtered, and concentrated to afford 6-methyl-7-(l-(l-(2,2,2- trifluoroethyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylic acid (20 mg yellow solid , 72% yield). LCMS (M + H+) m/z 362.
[00190] The following intermediates were synthesized in a similar manner to 7-(l-(l-
(2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5- carboxylic acid using the appropriate starting materials and modifications:
[00191] Step 4: Synthesis of 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4- yl)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 144).
[00192] A mixture of 6-methyl-7-(l-(l-(2,2,2-trifluoroethyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylic acid (20 mg, 0.055mmol), 3-(aminomethyl)- 4-methoxy-6-methylpyridin-2(lH)-one (12 mg, 0.065mmol), triethylamine (17 mg, 0.16 mmol), and (l-cyano-2-ethoxy-2-oxoethylidenaminooxy) dimethylamino-morpholino- carbenium hexafluorophosphate ( 29 mg, 0.066mmol) in DMF (3 mL) was stirred at 20 °C for 3 hours under nitrogen. The mixture was partitioned between with EtOAc and water and the layers separated. The organic layer was dried over Na2S04, filtered, and concentrated to
afford crude material. The crude material was purified by preparative-HPLC (Instrument: Gilson GX281 ; Condition: Column: Gemini 150*25mm*5um; Mobile phase A: water with 0.05% ammonia solution; Mobile phase B: MeCN; column temperature: 30°C; Gradient: B in A 47-77%) to afford 7-(l-(l-(2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl)-N-((4- methoxy-6-methyl-2-oxo- l,2-dihydropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b]pyridazine- 5-carboxamide (3.6 mg, 13% yield). LCMS (M + H+) m/z 512. 1H NMR (400 MHz, CD3OD): δ 8.15-8.13 (d, J = 9.2 Hz, 1H), 8.09-8.08 (t, J = 4.4 Hz, 1Η),6.70-6.66 (dd, Ji = 9.2 Hz, J2 = 9.2 Hz, 2H) , 6.26 (s, 1H) ,4.60 (s, 2H), 4.69 (s, 2H), 3.9 (s, 3H), 3.72 (s, 1H), 2.95 (s, 2H) , 2.77-2.74 (s, J = 12.0 Hz, 2H), 2.41 (s, 3H) ,2.35 (s, 1H), 2.30 (s, 3H), 1.97 (s, 1H), 1.40- 1.38 (d, J = 6.8Hz, 3H), 1.32-1.31 (d, J = 3.2Hz, 3H), 1.27-1.26 (d, J = 3.2Hz, 3H), 1.13 (m, 1H) , 1.01-0.93 (m, 2H).
[00193] The following compounds were synthesized in a similar manner to Compound g the appropriate intermediates:
Name 1H NMR LCMS
Number Used
lH NMR (400 MHz, MeOH-d4)
6-(l-(l-(2,2- 5 1.50 (m, 3H), 1.82-1.78 (m,
difluoropropyl)piperidin-4- 5H), 2.36-2.31 (m, 3H), 2.41 (s,
yl)ethyl)-N-((4-methoxy-6- 3H), 2.57 (s, 3H), 3.22-3.13 (m,
methyl-2-oxo-l ,2-
140
dihydropyridin-3- 3H), 3.56-3.42 (m, 1H), 3.78 (m, 516 11B, 19E yl)methyl)-7- 3H), 4.02 (s, 3H), 4.57 (m, 2H),
methylpyrrolo[ 1 ,2- 6.55 (s, 1H), 7.71-7.70 (m, 1H),
a]pyrazine- 8 -carboxamide 8.74 (m, 1H), 9.23 (s, 1H).
[00194] Example 21. Synthesis of 6-chloro-N-((4,6-dimethyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-7-(l-(l-(2-hvdroxy-2-methylpropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 141).
[00195] Step 1. Synthesis of ethyl 7-(l-((benzyloxy)carbonyl)piperidine-4- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
[00196] To a solution of benzyl 4-(2-bromoacetyl)piperidine-l-carboxylate (IF; 5.0g,
14.7mmol) in anhydrous (methylsulfinyl)methane (5ml) were added ethyl propiolate (1.73 g, 17.6mmol), potassium carbonate (5.07 g, 36.7mmol) and pyridazine (1.41g, 17.6 mmol). The reaction mixture was stirred at room temperature for 2.5 h. Then the reaction was quenched by water(20ml) and extracted with EtOAc ( 30mL x3 ) . The combined organic layer was dried over Na2S04 and concentrated to give the crude product and the crude product was purified by column chromatograph on silica gel (eluted: petrol ether / acetic ester=30: 1-5: 1) to afford ethyl 7-(l-((benzyloxy)carbonyl)piperidine-4-carbonyl)pyrrolo[l,2-b]pyridazine-5- carboxylate (1.9g, 30%) as a brown oil.
[00197] Step 2. Synthesis of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)-l- hydroxyethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
[00198] To a solution of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)-l- hydroxyethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (1.9 g, 6.27mmol) in anhydrous THF (22.5mL) was added methylmagnesium bromide solution (9.5 mL, 2mol/L) dropwise at -
65°C. The resulting mixture was stirred at -65°C for 2 hours. The reaction was quenched by saturated ammonium chloride (9 ml) at -20 °C. The aqueous phase was extracted with EtOAc (50ml x 3) and the combined organic layers were dried over Na2S04, filtered, and concentrated to give crude ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4-yl)-l- hydroxyethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate as a red oil. It was used directly in the next step.
[00199] Step 3. Synthesis of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
[00200] To a solution of ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (1.97g, 4.36mmol) in anhydrous dichloromethane (6.5 mL) was added triethylsilane (1.2 mL), trifluoroacetic acid (1.2 mL) at -20 °C. The reaction mixture was stirred at -10°C for 1 h. Then the reaction mixture was quenched by saturated sodium bicarbonate solution and extracted with EtOAc (40ml x 3). The combined organic layer was dried over Na2S04, filtered, and concentrated to give crude product. The crude product was purified by column chromatograph on silica gel (eluted: petrol ether / acetic ester=35: 1-20: 1) to afford ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-
4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (1.53g, 82% yield) as a yellow oil. LCMS (M+H+) m/z 436.
[00201] Step 4. Synthesis of ethyl 7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine- - carboxylate.
[00202] A mixture of compound ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (2.0 g, 4.6 mmol) and Pd/C(54.7mg) in MeOH (30 mL) was stirred under hydrogen (latm) at room temperature for 18 hours. The reaction mixture was filtered and the filtrate was concentrated to give crude product. The crude product was used directly in the next step.
[00203] Step 5. Synthesis of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
[00204] To a solution of ethyl 7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5- carboxylate (1.38g, 4.59 mmol) in anhydrous dichloromethane (20 mL) were added triethylamine (926.7 mg, 9.16 mmol) and di-tert-butyl dicarbonate (2.0g,9.16mmol) . The reaction mixture was stirred at room temperature overnight. The reaction was quenched by water (20 mL) and extracted with EtOAc(100 mLx3). The combined organic layers were dried over Na2S04, filtered, and concentrated. The crude product was purified by column chromatograph on silica gel (eluted: petrol ether / acetic ester=35: 1-25: 1) to give ethyl7-(l- ( l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)pyrrolo[ 1 ,2-b]pyridazine-5-carboxylate ( 1.46 g, 79% yield). LCMS (M + H+) m/z 302.
[00205] Step 6. Synthesis of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)- -dichloropyrrolo[ 1,2-b ]pyridazine-5-carboxylate.
[00206] To a solution of compound ethyl7-(l-(l-(tert-butoxycarbonyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (l.Og, 2.5mmol) in anhydrous DMF was added NCS (655.2mg, 5mmol) in portions at -30 °C. The reaction mixture was stirred at room temperature for 10 hours. The reaction mixture was quenched by water and extracted with EtOAc (30mL x 3). The organic layer was dried over Na2S04, filtered, and concentrated to give the crude product, The crude product was used directly in the next step.
[00207] Step 7. Synthesis of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)- -chloropyrrolo[l,2-b]pyridazine-5-carboxylate.
[00208] To a solution of compound ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4- yl)ethyl)-3,6-dichloropyrrolo[l,2-b]pyridazine-5-carboxylate (463.4mg, 1.07mmol) in anhydrous MeOH (20ml) was added palladium on carbon (29.3mg, 0.25mmol, 10 wt% Pd). The reaction mixture was purged with H2 (latm) and allowed to stirred at room temperature
for 8 hours. Then the mixture was filtered and concentrated to give the crude product. The crude product was purified by column chromatograph on silica gel (eluted: petrol ether / acetic ester=25: 1-5: 1) to give ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)-6- chloropyrrolo[l,2-b]pyridazine-5-carboxylate (100 mg, yield 24%) as a yellow oil.
[00209] Step 8: Synthesis of ethyl 6-chloro-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2- b ]pyridazine-5-carboxylate
[00210] The compound of ethyl 7-(l-(l-(tert-butoxycarbonyl)piperidin-4-yl)ethyl)-6- chloropyrrolo[l,2-b]pyridazine-5-carboxylate (100mg,0.23mmol) was dissolved in hydrogen chloride (10ml) in MeOH, and the mixture was stirred at room temperature for 2 hours. Then the mixture was concentrated to give crude ethyl 6-chloro-7-(l-(piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate. It was used directly in the next step.
[00211] Step 9: Synthesis of ethyl 6-chloro-7-(l-(l-(2-hydroxy-2- methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate.
[00212] To a solution of crude compound ethyl 6-chloro-7-(l-(piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (77 mg, 0.23mmol) in anhydrous MeOH (3ml) was added N-ethyl-N-isopropylpropan-2-amine (118.7 mg, 0.92 mmol) and 2,2- dimethyloxirane (66.34 mg, 0.92mmol) at room temperature. The reaction mixture was stirred at 45 °C overnight. The reaction mixture was concentrated to give the crude ethyl 6- chloro-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5- carboxylate. The crude product was used directly in the next step. LCMS (M+H+) mJz 408.
[00213] Step 10: Synthesis of 6-chloro-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin- -yl)ethyl)pyrrolo[ 1,2-b Jpyridazine-5-carboxylic acid.
[00214] To a solution of crude compound ethyl 6-chloro-7-(l-(l-(2-hydroxy-2- methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (93.5 mg, 0.23
mmol) in MeOH (3.5ml) and water (3.5ml) was added sodium hydroxide (46 mg, 1.15 mmol) at room temperature. The reaction mixture was stirred at 70 °C for 3.5 hours. Then the reaction mixture was acidified to pH=3 with 2N hydrogen chloride and concentrated to give the crude 6-chloro-7-( 1 -( 1 -(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)pyrrolo[ 1 ,2- b]pyridazine-5-carboxylic acid. LCMS (M+H+) m/z 380.
[00215] Step 11: Synthesis of 6-chloro-N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2- b]pyridazine-5-carboxamid (Compound 141).
[00216] To a solution of crude compound 6-chloro-7-(l-(l-(2-hydroxy-2- methylpropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylic acid (88.75 mg, 0.23 mmol) in anhydrous DMF (2.5 ml) were added l-cyano-2-ethoxy-2- oxoethylidenaminooxy)dimethylamino-morpholino-carbenium hexafluorophosphate (395.6 mg, 0.92 mmol) and N-ethyl-N-isopropylpropan-2-amine (118.7 mg, 0.92 mmol) at room temperature. The reaction mixture was stirred at room temperature for 10 min. Then 3- (aminomethyl)-4,6-dimethylpyridin-2(lH)-one (44mg,0.23mmol) was added at room temperature and the reaction mixture stirred at room temperature for overnight. The reaction was concentrated and the resulting crude product was purified by preparative HPLC (Mobile phase A : water with 0.05% ammonia solution; Mobile phase B : MeCN; column temperature: 30°C, Gradient: 50-80% B 10 min) to give 6-chloro-N-((4,6-dimethyl-2-oxo- l,2-dihydropyridin-3-yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (31.1 mg, 26% yield). LCMS (M+H+) m/z 514. 1H NMR (400 MHz, CHLOROFORM- J) δ 12.15 (s., 1 H), 8.74 (dd, J=9.03, 1.51 Hz, 1 H), 8.11 (dd, J=4.52, 1.51 Hz, 1 H), 7.96 (t, J=5.52 Hz, 1 H), 6.73 (dd, J=9.29, 4.27 Hz, 1 H), 5.94 (s, 1 H), 4.59 (d, J=5.52 Hz, 2 H), 3.32 (s., 1 H), 3.01 (s., 1 H), 2.76 (s., 1 H), 2.41 (s, 4 H), 2.30 (s, 5 H), 2.18 (s., 1 H), 2.11-2.04(m, 1 H), 1.98 (d, J=13.05 Hz, 2 H), 1.40 (d, J=7.03 Hz, 4 H), 1.26 (s, 1 H), 1.12 (s, 3 H), 1.15 (s, 3 H), 1.01 (d, J=12.55 Hz, 1 H)
[00217] Compound 142 was synthesized in a similar manner to Compound 141 using the appropriate starting materials and modifications:
Compound
Name 1H NMR LCMS No.
lH NMR (400 MHz, CHLOROFORM-J) δ
6-chloro-7-(l-(l-(2- 12.95 (s, 1 H), 8.82-8.74 (m, 1 H), 8.10 (dd,
hydroxy-2- J=4.52, 1.51 Hz, 1 H), 7.98 (t, J=5.27 Hz, 1 methylpropyl)piperidin-4- H), 6.72 (dd, J=9.03, 4.52 Hz, 1 H), 5.95 (s, 1 yl)ethyl)-N-((4-methoxy- H), 4.63 (d, J=5.52 Hz, 2 H), 3.90 (s, 3 H),
142 6-methyl-2-oxo- 1 ,2- 3.32 (s, 1 H), 3.09 (s, 1 H), 2.84 (s, 1 H), 2.44 530 dihydr op yridin- 3 - (s., 1 H), 2.38 (s, 5 H), 2.28-2.20(m, 1 H), yl)methyl)pyrrolo[ 1 ,2- 2.12 (d, J=9.03 Hz, 1 H), 2.00 (d, J=12.05
b]pyridazine-5- Hz, 1 H), 1.40 (d, J=7.53 Hz, 3 H), 1.25 (s., 2 carboxamide H), 1.17 (s, 3 H), 1.14 (s, 3 H), 1.03 (d,
J=13.05 Hz, 1 H)
[00218] Example 22. Synthesis of 7-tert-butyl 5-ethyl 6-methylpyrrolori.2- b]pyridazine-5,7-dicarboxylate (Compound 125)
[00219] Step 1: Synthesis of 7-tert-butyl 5-ethyl 6-methylpyrrolo[l,2-b]pyridazine- 5 7-dicarboxylate
[00220] To a solution of pyridazine (2.0 g, 25.0 mmol) in dimethyl sulfoxide (100.0 mL) was added ethyl but-2-ynoate (2.8 g, 25.0 mmol), tert-butyl 2-bromoacetate (4.87 g, 25.0 mmol) and potassium carbonate (6.9 g, 50.0 mmol). The resulting solution was stirred at 30 °C under N2 for 8 hours. The mixture was partitioned between ethyl acetate and water. The organic phase was removed and the aqueous layer was extracted with ethyl acetate. The combined organic layers were washed by brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue was purified by silica gel chromatography (petroleum ether : ethyl acetate = 12: 1) to afford 7-tert-butyl 5-ethyl 6-methylpyrrolo[l,2-b]pyridazine- 5,7-dicarboxylate (620.0 mg, 8.2%) as a light yellow solid.
[00221] Step 2: Synthesis of 5-(ethoxycarbonyl)-6-methylpyrrolo[l,2-b]pyridazine-7- rboxylic acid
[00222] To a solution of 7-tert-butyl 5-ethyl 6-methylpyrrolo[l,2-b]pyridazine-5,7- dicarboxylate (300.0 mg, 0.99 mmol) in dichloromethane (18.5 mL) was added triethylamine (3.0 g, 29.7 mmol). To the reaction mixture was added trimethylsilyl trifluoromethanesulfonate (4.4 g, 19.8 mmol) in a dropwise manner. The resulting solution was stirred at room temperature under N2 for 2 hours. The reaction was quenched by the addition of water and the mixture was concentrated in vacuo to afford the crude product which was in the next step without further purification.
[00223] Step 3: Synthesis of ethyl 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (Intermediate 20 A)
[00224] To a solution of 5-(ethoxycarbonyl)-6-methylpyrrolo[l,2-b]pyridazine-7- carboxylic acid (150.0 mg, 0.6 mmol) in N,N-dimethylformamide (3.0 mL) was added 0-(7- azabenzotriazol-l-yl)-N,N,N,N,-tetramethyluronium (456.0 mg, 1.2 mmol), N,N- diisopropylethylamine (232.2 mg, 1.8 mmol) and morpholine (104.5 mg, 1.2 mmol). The resulting solution was stirred at 30 °C under N2 overnight. The mixture was partitioned between ethyl acetate and water. The organic phase was removed and the aqueous layer was extracted with ethyl acetate. The aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue was purified by silica gel pad (dichloromethane : methanol = 30: 1) to afford ethyl 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (120.0 mg, 62.6%) as a yellow oil.
[00225] The intermediate shown in the following table was prepared according to the procedure described above using the appropriate starting materials and modifications:
[00226] Step 4: Synthesis of 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylic acid (Intermediate 21 A)
[00227] To a solution of ethyl 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (120.0 mg, 0.38 mmol) in tetrahydrofuran/methanol/water (2.0 mL/2.0 mL/2.0 mL) was added sodium hydroxide (80.0 mg, 2.0 mmol). The reaction mixture was stirred at 50 °C overnight. To the resultant reaction mixture was added IN hydrogen chloride until the reaction mixture was adjusted to pH 5-6. The mixture was subsequently concentrated in vacuo to afford 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylic acid (96.0 mg, 87.7%) as yellow oil which was used for the next step directly.
[00228] Step 5: Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxamide
( Compound 125)
[00229] To a solution of 6-methyl-7-(morpholine-4-carbonyl)pyrrolo[l,2-b]pyridazine-
5-carboxylic acid (96.0 mg, 0.33 mmol) in N,N-dimethylformamide (2.5 mL) was added O- (7-azabenzotriazol-l-yl)-N,N,N,N,-tetramethyluronium (250.8 mg, 0.66 mmol), N,N- diisopropylethylamine (127.7 mg, 0.99 mmol) and 3-(aminomethyl)-4,6-dimethylpyridin-
2(lH)-one (150.7 mg, 0.99 mmol). The resulting solution was stirred at room temperature under N2 overnight. The mixture was partitioned between ethyl acetate and water. The organic phase was removed and the aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over anhydrous sodium sulfate, and concentrated in vacuo. The residue was subjected to preparatory HPLC for purification to afford N-((4,6-dimethyl-2-oxo- 1 ,2-dihydropyridin-3-yl)methyl)-6-methyl-7-(morpholine-4- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (17.0 mg, 12.1%) as a light yellow solid. LCMS (M + H+) m/z: calcd. 423.47, found 423.9. 1H NMR (400 MHz, DMSO-d6) δ 11.54 (br.s, 1H), 8.34 - 8.26 (m, 2H), 7.91 - 7.83 (m, 1H), 6.96 - 6.89 (m, 1H), 5.89 (s, 1H), 4.34 - 4.26 (m, 2H), 3.81 - 3.49 (m, 6H), 3.16 - 3.04 (m, 2H), 2.36 (s, 3H), 2.25 (s, 3H), 2.11 (s, 3H).
[00230] Example 23. Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihvdropyridin-3- yl)methyl)-6-methyl-7-(4-(methylsulfonyl)piperazine-l-carbonyl)pyrrolori,2- blpyridazine-5-carboxamide (Compound 126)
[00231] Step 1: Synthesis of ethyl 6-methyl-7-(piperazine-l-carbonyl)pyrrolo[l,2- b ]pyridazine-5-carboxylate
[00232] To a solution of ethyl 7-(4-(tert-butoxycarbonyl)piperazine-l-carbonyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (20B; 394.0 mg, 0.94 mmol) in HCI in methanol (4.0 mL) was stirred at room temperature under N2 for 2 hours. The reaction mixture was concentrated in vacuo to afford the crude product which was used for the next step directly.
[00233] Step 2: Synthesis of ethyl 6-methyl-7-(4-(methylsulfonyl)piperazine-l- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxyl te
[00234] To a cooled (0 °C) solution of ethyl 6-methyl-7-(piperazine-l- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (299.5 mg, 0.95 mmol) in dichloromethane
(5.0 mL) was added triethylamine (479.8 mg, 4.75 mmol), followed by the addition of methanesulfonyl chloride (130.4 mg, 1.14 mmol). The resulting solution was stirred at room temperature under N2 overnight. The mixture was poured into ice water and extracted with ethyl acetate. The aqueous phase was further extracted with ethyl acetate. The combined organic layers were washed by brine, dried over anhydrous sodium sulfate, and concentrated in vacuo to afford ethyl 6-methyl-7-(4-(methylsulfonyl)piperazine-l-carbonyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (277.8 mg, 100%) as yellow oil which was used for the next step without further purification.
[00235] Step 3: Synthesis of 6-Methyl-7-(4-(methylsulfonyl)piperazine-l- carbonyl)pyrrolo[l,2-b ]pyridazine-5-carboxylic acid
[00236] Ethyl 6-methyl-7-(4-(methylsulfonyl)piperazine- l-carbonyl)pyrrolo[ 1 ,2- b]pyridazine-5-carboxylate was converted to 6-methyl-7-(4-(methylsulfonyl)piperazine-l- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylic acid as described in Step 4 of the previous Example.
[00237] Step 4: Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-6-methyl-7-(4-(methylsulfonyl)piperazine-l-carbonyl)pyrrolo[l,2-b]pyridaz -carboxamide (Compound 126)
6-methyl-7-(4-(methylsulfonyl)piperazine-l-carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylic acid was combined with 3-(aminomethyl)-4,6-dimethylpyridin-2(lH)-one to produce Compound 126 as described in Step 5 in the previous Example. LCMS (M + H+) m/z:501. 1H NMR (400 MHz, DMSO-d6) δ 11.60 (s, 1H), 8.45-8.18 (m, 2H), 7.95 - 7.81 (m, 1H), 7.04
- 6.88 (m, 1H), 5.89 (s, 1H), 4.37 - 4.23 (m, 2H), 3.95 - 3.64 (m, 2H), 3.27-3.16 (m, 4H), 3.14
- 2.97 (m, 2H), 2.93 (s, 3H), 2.37 (s, 3H), 2.25 (s, 3H), 2.12 (s, 3H).
[00238] Example 24. Synthesis of ethyl 2.6-dimethyl-7-(l-(piperidin-4- yl)ethyl)pyrrolori,2-b1pyridazine-5-carboxylate (Compound 129):
[00239] Step 1: Synthesis of ethyl 2,6-dimethyl-7-(l-(piperidin-4- yl)ethyl)pyrrolo[l,2-b ]pyridazine-5-carboxylate (Intermediate 21 A )
[00240] A mixture of compound ethyl 7-(l-(l-((benzyloxy)carbonyl)piperidin-4- yl)ethyl)-2,6-dimethylpyrrolo[l,2-b]pyridazine-5-carboxylate (20C; 220 mg, 0.48 mmol) and Pd/C (40mg) in methanol (5 mL) was stirred under hydrogen (15 psi) at room temperature for 2 hours. The reaction mixture was filtered and the filtrate was concentrated to give crude product. The crude product was purified by preparatory TLC(dichloromethane : methanol = 10 : 1) to give ethyl 2,6-dimethyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5- carboxylate (110 mg, 69.6% yield). LCMS (M+H+) m/z: calcd 329.1; found 330.1
[00241] The intermediate shown in the following table was prepared according to the procedure described above using 20D as the starting material:
[00242] Step 2: Synthesis of ethyl 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2, 6-dimethylpyrrolo[l,2-b Jpyridazine-5-carboxylate (Intermediate 22 A )
[00243] To a solution of ethyl 2,6-dimethyl-7-(l-(piperidin-4-yl)ethyl)pyrrolo[l,2- b]pyridazine-5-carboxylate (50 mg, 0.15 mmol) in methanol (2 mL) was added 2,2- dimethyloxirane (32 mg, 0.45 mmol) and N-ethyl-N-isopropylpropan-2-amine (116 mg, 0.9 mmol). The reaction mixture was stirred at 40 °C overnight. The reaction was completed and concentrated to give crude product ethyl 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2,6-dimethylpyrrolo[l,2-b]pyridazine-5-carboxylate (30 mg, 50% yield). LCMS (M +H+) m/z: calcd 401.1; found 401.9.
[00244] The intermediate shown in the following table was prepared according to the procedure described above using the appropriate starting material:
[00245] Step 3: Synthesis of 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (Intermediate 23 A)
[00246] To a solution of compound ethyl 7-(l-(l-(2-hydroxy-2- methylpropyl)piperidin-4-yl)ethyl)-2,6-dimethylpyrrolo[ 1 ,2-b]pyridazine-5-carboxylate (30 mg, 0.08 mmol) in methanol (2 mL) and water (1 mL) was added sodium hydroxide (15 mg, 0.38 mmol). The reaction mixture was stirred at 70 °C overnight. The reaction mixture was neutralized with 2N hydrochloride and the resultant solution was concentrated to give the crude product 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2,6- dimethylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (15 mg, 53.4% yield)as a yellow solid. LCMS (M+H+) m/z: calcd 373.2; found 373.9.
[00247] The intermediate shown in the following table was prepared according to the procedure described above using the appropriate starting materials:
[00248] Step 4: Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-6-methylpyrro b Ipyridazin e-5- carb oxam ide (Compound 129)
[00249] To a solution of compound 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2,6-dimethylpyrrolo[l,2-b]pyridazine-5-carboxylic acid (15mg, 0.04mmol) and 3- (aminomethyl)-4,6-dimethylpyridin-2(lH)-one (8.5mg, 0.06mmol) in dichloromethane (3 mL) were added compound 0-(7-Azabenzotriazol-l-yl)-N,N,N',N'-tramethyluronium hexafluorophosphate (28 mg, 0.07mmol) and triethylamine (7.4 mg, 0.07 mmol). The reaction mixture was stirred at 50 °C for 3 hours. The crude product was purified by preparative HPLC (Mobile phase A : water with 0.05% ammonia solution; Mobile phase B: MeCN; column temperature: 30 °C, Gradient: 30-60% B over 10 min) to give product N- ((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-7-(l-(l-(2-hydroxy-2- methylpropyl)piperidin-4-yl)ethyl)-2,6-dimethylpyrrolo[l,2-b]pyridazine-5-carboxamide (12 mg, 63%) as a yellow solid. LCMS (M+H+) calcd. 507.32; found 508.1. 1H NMR (400 MHz, CDC13) δ 0.96-1.07 (m, IH), 1.31-1.32 (d, J=3.2 Hz, 6H), 1.34-1.36 (d, J=7.2 Hz, 4H), 1.94- 2.20 (m, 3H), 2.23 (s, 6H), 2.40-2.43 (m, 8H), 2.49 (s, IH), 2.78 (s, IH), 2.93 (s, 2H), 4.54- 4.56 (d, J=5.6Hz, 2H), 5.94 (s, IH), 6.58 (m, IH), 7.20 (m, lH),8.26-8.28(d, J=9.2Hz, IH).
[00250] The compound of the invention shown in the following table was prepared according to the procedure described above using the indicated starting materials and art-known modifications.
[00251] Example 25. Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihvdropyridin-3- yl)methyl)-7-(l-(l-(2-hvdroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2-methoxy-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 127):
[00252] Step 1: Synthesis of ethyl 2-methoxy-6-methyl-7-(l-(l-(2- oxopropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate
[00253] To a solution of ethyl 2-methoxy-6-methyl-7-(l-(piperidin-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (21B; 210 mg, 0.61 mmol) in N,N- dimethylformamide (10 mL) were added l-chloropropan-2-one (84 mg, 0.91mmol) and potassium carbonate (252 mg, 1.83mmol). The reaction mixture was stirred at 50 °C for 3 hours. The reaction was quenched by adding water (20 mL). The organic phase was removed and the aqueous phase was extracted with ethyl acetate (10 mL x 3). The combined organic layers were concentrated to give crude product ethyl 2-methoxy-6-methyl-7-(l-(l-(2- oxopropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (200 mg, 81.9% yield). LCMS (M+H+) m/z: calcd 401.2 found 401.9
[00254] Step 2: Synthesis of ethyl 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2-methoxy-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate
[00255] To a cooled (-78 °C) solution of ethyl 2-methoxy-6-methyl-7-(l-(l-(2- oxopropyl)piperidin-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (200 mg, 0.50 mmol) in tetrahydrofuran (5 mL) was added methylmagnesium bromide solution (0.85 mL, 3 M). The resultant mixture was stirred at -30 °C for 3 h. The reaction was subsequently quenched with saturated aqueous ammonium chloride (100 mL). The organic phase was removed and aqueous phase was extracted with ethyl acetate (10 mL x 3). The combined organic layers were dried over sodium sulfate and concentrated to give the crude product ethyl 7-(l-(l-(2- hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2-methoxy-6-methylpyrrolo[l,2-b]pyridazine- 5-carboxylate (200 mg, 96.1% yield) as a yellow oil.
[00256] Step 3: Synthesis of 7-(l-(l-(2-Hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2-methoxy-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid
[00257] The conversion of ethyl 7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4- yl)ethyl)-2-methoxy-6-methylpyrrolo[l,2-b]pyridazine-5-carboxylate to 7-(l-(l-(2-Hydroxy-
2-methylpropyl)piperidin-4-yl)ethyl)-2-meth^
carboxylic acid was achieved as described in Step 3 of the previous Example.
[00258] Step 4: Synthesis of N-((4,6-dimethyl-2-oxo-l,2-dihydropyridin-3- yl)methyl)-7-(l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2-methoxy-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (Compound 127).
[00259] 7-(l-(l-(2-Hydroxy-2-methylpropyl)piperidin-4-yl)ethyl)-2-methoxy-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylic acid was combined with 3-(aminomethyl)-4,6- dimethylpyridin-2(lH)-one to produce Compound 127 as described in Step 4 in the previous Example. LCMS (M + H+) m/z: 524. 1H NMR (400 MHz, CDC13) δ 1.11-1.08 (d, J=12 Hz, 6H), 1.34 (m, 4H), 1.96 (m, 3H), 2.11-2.08 (d, J=12 Hz, 6H), 2.26 (m,6H), 2.40 (m, IH), 2.97-2.92 (d, J=20 Hz, 4H), 3.34 (s, IH), 3.90 (s, 3H), 4.54-4.53 (d, J=4 Hz, 2H), 5.91 (s, IH), 6.29-6.27 (d, J=8 Hz, IH), 7.16 (m, IH), 8.30-8.27 (d, J=12 Hz, IH).
[00260] Example 26. Synthesis of 7-(l-(dimethylamino)ethyl)-N-((4-methoxy-6- methyl-2-oxo-l,2-dihvdropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b1pyridazine-5- carboxamide (Compound 130)
[00261] Step 1: Synthesis of Ethyl 7-(l-(dimethylamino)ethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylate The intermediates shown in the following table were prepared using intermediate 4G and the appropriate amine according to the procedure described in Step 1 of Example
9.
[00262] Step 1A: Synthesis of ethyl 7-(l-(ethyl(methyl)amino)ethyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxylate (Intermediate 25B)
[00263] To a solution of ethyl-6-methyl-7-(l-(methylamino)ethyl)pyrrolo[l,24j]pyridazine-5- carboxylate (24B; 300 mg, 1.15 mmol) in acetic acid (5 mL) was sequentially added acetaldehyde (253 mg, 5.75 mmol) and sodium cyanoborohydride (214 mg, 3.45mmol). The resultant reaction mixture was stirred at room temperature overnight. After 24 h, the reaction mixture was concentrated and purified on silica gel eluted with (dichloromethane / methanol 50: 1→20: 1) to give product ethyl 7-(l-(ethyl(methyl)amino)ethyl)-6-methylpyrrolo[l,243]pyridazine-5-carboxylate (150 mg, 45% yield) as a yellow oil. LCMS (M+H+) m/z: calcd. 289.1, found 290.1
[00264] Step 2: Synthesis of 7-(l-(Dimethylamino)ethyl)-6-methylpyrrolo[l,2-b]pyridazine-
5-carboxylic acid (Intermediate 26A). The intermediates shown in the following table were prepared according to the dealkylation procedure described in Example 12 using the appropriate starting materials and modifications.
[00265] Step 3: Synthesis of 7-(l-(dimethylamino)ethyl)-N-((4-methoxy-6-methyl-2- oxo-l,2-dihydropyridin-3-yl)methyl)-6-methylpyrrolo[l,2-b]pyridazine-5-carboxam
( Compound 130)
To a solution of 7-(l-(dimethylamino)ethyl)-6-methylpyrrolo[l,2- b]pyridazine-5-carboxylic acid (26A; 40 mg, 0.16 mmol) in N,N-dimethylformamide (2 mL) were added 3-(aminomethyl)-4-methoxy-6-methylpyridin-2(lH)-one (40.8 mg, 0.24 mmol),
N-ethyl-N-isopropylpropan-2-amine (62.6 mg, 0.48 mmol), and (l-Cyano-2-ethoxy-2- oxoethylidenaminooxy)dimethylamino-morpholino-carbenium hexafluorophosphate ( 102.7 mg, 0.24mmol). The mixture was stirred at room temperature overnight. The reaction mixture was concentrated and purified by preparative HPLC to give product 7-(l- (dimethylamino)ethyl)-N-((4-methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-6- methylpyrrolo[l,2-b]pyridazine-5-carboxamide (5.5 mg, 8.5%) as a yellow solid. LCMS (M+H+) m/z: calcd. 397.21, found 398.2. 1H NMR (400 MHz, CDC13) δ 1.66-1.64 (d, J=8 Hz, 3H), 2.36 (m, 9H), 2.60 (s, 3H), 3.90 (s, 3H), 4.40 (s, 1H), 4.63-4.62 (d, J=12 Hz, 2H), 5.95 (s, 1H), 6.65 (m, 1H), 7.41 (s, 1H), 8.09 (m, 1H), 8.49 (m, 1H).
[00267] The compound of the invention shown in the following table was prepared according to the procedure described above using the indicated starting materials and art- known modifications.
[00268] Example 27. Synthesis of 6-chloro-N-((4-methoxy-6-methyl-2-oxo-l,2- dihvdropyridin-3-yl)methyl)-7-(l-(tetrahvdro-2H-pyran-4-yl)ethyl)pyrrolo[l,2- blpyridazine-5-carboxamide (Compound 132)
[00269] Steps 1-3.Synthesis of ethyl 7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate. 2-bromo-l-(tetrahydro-2H-pyran-4- yl)ethanone (from Example 3, step 2) was combined with methyl but-2-ynoate (3) and pyridazine (2) as described in Example 4 to produce ethyl 7-(tetrahydro-2H-pyran-4- carbonyl)pyrrolo[l,2-b]pyridazine-5-carboxylate. That intermediate was treated with methylmagnesium bromide in THF as described in Example 8, step 1 to produce ethyl 7-(l- hydroxy-l-(tetrahydro-2H-pyran-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate. The hydroxyl intermediate was then treated with Et SiH dissolved in THF, followed by TFA, as
described in Example 8, step 2 to produce ethyl 7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[ 1 ,2-b]pyridazine-5-carboxylate.
[00270] Step 4: Synthesis of ethyl 3,6-dichloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate
[00271] To a cooled (-30 °C) solution of ethyl 7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (2.5 g, 8.25 mmol) in anhydrous N,N- dimethylformamide (50 mL) was added l-chloropyrrolidine-2,5-dione (2.2 g, 16.5 mmol) in portions. The resulting mixture was allowed to stir at room temperature for 6 h. The reaction mixture was quenched with water (200 mL), and then extracted with ethyl acetate (200 mL x 3). The combined organic phases were dried by anhydrous sodium sulphate, and concentrated in vacuo. The resultant oil was purified by column chromato graph on silica gel (eluted with petroleum ether / ethyl ester 10: 1 to 5: 1) to give ethyl 3,6-dichloro-7-(l-(tetrahydro-2H- pyran-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (2.3 g, 75% yield) as a yellow oil. LCMS (M+H+) m/z: calcd. 371.05, found 371.9.
[00272] Step 5: Synthesis of ethyl 6-chloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate
[00273] To a solution of ethyl 3,6-dichloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (1.5 g, 4.9 mmol) in ethyl acetate (50 mL) was added 10% palladium on carbon (75 mg, 0.63 mmol) in portions. Then the mixture was purged with hydrogen (latm) and allowed to stir at room temperature for 5 h. After 5 h, the reaction mixture was filtered. The filtrate was concentrated and purified by column chromato graph on silica gel (eluted with petroleum ether / ethyl acetate 10: 1 to 5: 1) to give ethyl 6-chloro-7-(l-(tetrahydro-2H-pyran-4-yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (1.3 g, 96% yield) as a yellow solid. LCMS (M+H+) m/z: calcd. 336.35, found 336.9.
[00274] Step 6: Synthesis of 6-chloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b ]pyridazine-5-carboxylic acid
[00275] To a solution of ethyl 6-chloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylate (1.3 g, 3.86 mmol) in tetrahydrofuran/methanol (1: 1, 40 mL) was added a solution of sodium hydroxide (772 mg, 19.3 mmol) dissolved in 20 mL water. The resulting reaction system was heated to 70 °C and allowed to stir for 5 h. After 5 h, the reaction mixture was cooled to 0 °C and 4N hydrogen chloride was added until the reaction solution turned slightly acidic (pH 6). Solvent and water was removed under reduced pressure to give 6-chloro-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxylic acid (1.19 g, assume quantitative yield) as a yellow solid, and it was used directly in the next step. LCMS (M+H+) m/z: calcd. 308.08, found 308.9.
[00276] Step 7: Synthesis of 6-chloro-N-((4-methoxy-6-methyl-2-oxo-l,2- dihydropyridin-3-yl)methyl)-7-(l-(tetrahydro-2H-pyran-4-yl)ethyl)pyrrolo[l,2-
b]pyridazine-5-carboxylic acid (1.19 g, crude) in anhydrous N,N-dimethylformamide (20 mL) was added l-cyano-2-ethoxy-2-oxoethylidenaminooxy)dimethylamino-morpholino- carbenium hexafluorophosphate (2.48 g, 5.79 mmol) and N-ethyl-N-isopropylpropan-2- amine (996 mg, 7.72 mmol). The mixture was allowed to stirred for 30 mins at 0 °C, followed by addition of 3-(aminomethyl)-4-methoxy-6-methylpyridin-2(lH)-one hydrochloride (1.18 g, 5.79 mmol). The resultant reaction mixture was allowed to stir for 12 hours at room temperature. The reaction mixture was partitioned between water (50 mL) and dichloromethane. The organic phase was removed and the aqueous phase was extracted with dichloromethane (50 mL x 3). The combined organic phases were dried over anhydrous
sodium sulfate and concentrated in vacuo. The resultant oil was purified by preparative HPLC (Mobile phase A: water with 0.05% ammonia solution; Mobile phase B: MeCN; column temperature: 30 °C Gradient: 37-44% B over 10 min) to give 6-chloro-N-((4- methoxy-6-methyl-2-oxo-l,2-dihydropyridin-3-yl)methyl)-7-(l-(tetrahydro-2H-pyran-4- yl)ethyl)pyrrolo[l,2-b]pyridazine-5-carboxamide (462.1 mg, 26% yield) as a yellow solid. LCMS (M+H+) m/z: calcd. 458.17, found 459.1. 1H NMR (400 MHz, CDC13) δ 0.96 (m, 1H), 1.21-1.18 (m, 1H), 1.39-1.36 (m, 1H), 1.42 (d, J=7.2, 3H), 1.94-1.91 (m, 1H), 2.37 (m, 1H), 2.40 (s, 3H), 3.25-3.22 (m, 1H), 3.38 (m, 1H), 3.43-3.40 (m, 1H), 3.77 (m, 1H), 3.91 (s, 3H), 4.04-4.01 (m, 1H), 4.66 (d, J=1.2, 2H), 5.96 (s, 1H), 6.75-6.72 (m, 1H), 8.04-8.02 (m, 1H), 8.13-8.12 (m, 1H), 8.82-8.79 (m, 1H), 13.17 (s, 1H).
[00278] Example 28. IC¾n measurements for Inhibitors using EZH2.
[00279] EZH2 Assay: Assays were carried out by mixing rPRC2 together with biotinylated oligonucleosome substrates in the presence of the radio-labeled enzyme co-factor, S-adenosyl-L-methionine ( H SAM) (Perkin Elmer) and monitoring the enzymatically mediated transfer of tritiated methyl groups from H SAM to histone lysine residues. The amount of resulting tritiated methyl histone product was measured by first capturing the biotinylated oligonucleosomes in streptavidin (SAV) coated FlashPlates (Perkin Elmer), followed by a wash step to remove unreacted H SAM, and then counting on a TopCount NXT 384 well plate scintillation counter (Perkin Elmer). The final assay conditions for EZH2 were as follows: 50 mM Tris Buffer pH 8.5, 1 mM DTT, 69 μΜ Brij-35 detergent, 5.0 mM MgCl2, 0.1 mg/mL BSA, 0.2 μΜ 3H SAM, 0.2 μΜ biotinylated oligonucleosomes, 3.6 μΜ H3K27me3 peptide and 2 nM EZH2.
[00280] Compound IC50 measurements were obtained as follows: Compounds were first dissolved in 100% DMSO as 10 mM stock solutions. Ten point dose response curves were generated by dispensing varying amounts of the 10 mM compound solution in 10 wells of the 384 well plate (Echo; Labcyte), pure DMSO was then used to backfill the wells to insure all wells have the same amount of DMSO. A 12.5 μΐ^ volume of the HMT enzyme, H3K27me3 peptide and oligonucleosome substrate in assay buffer was added to each well of the assay plate using a Multidrop Combi (ThermoFisher). Compounds were pre-incubated with the enzyme for 20 min, followed by initiation of the methyltransferase reaction by addition of 12.5 μΐ^ of H SAM in assay buffer (final volume = 25 μί). The final concentrations of compounds ranged from a top default concentration of 80 μΜ down to 0.16 μΜ in ten 2-fold dilution steps. Reactions were carried out for 60 minutes and quenched with 20 μΐ^ per well
of 1.96 niM SAH, 50 niM Tris pH 8.5, 200 niM EDTA. Stopped reactions were transferred to SAV coated Flashplates (Perkin Elmer), incubated for 120 min, washed with a plate washer, and then read on the TopCount NXT (1.0 min/well) to measure the amount of methyl histone product formed during the reaction. The amount of methyl histone product was compared with the amount of product formed in the 0% and 100% inhibition control wells allowing the calculation of % Inhibition in the presence of the individual compounds at various concentrations. ICso's were computed using a 4 parameter fit non-linear curve fitting software package (XLFIT, part of the database package, ActivityBase (IDBS)) where the four parameters were IC50, Hill slope, pre-transitional baseline (0% INH), and post-transitional baseline (100% INH); with the latter two parameters being fixed to zero and 100%, respectively, by default.
[00281] Assay for Y641N EZH2 was performed as above using reconstituted H3K27Me2 oligonucleosomes as substrate.
[00282] Table 2 shows the activity of selected compounds of this invention in the EZH2 and Y641N EZH2 activity inhibition assay. IC50 values are reported as follows: "A" indicates an IC50 value of less than 100 nM; "B" indicates an IC50 value of 100 nM to 1 μΜ; "C" indicates an IC50 value of greater than 1 μΜ and less than 10 μΜ for each enzyme; "D" indicates an IC50 value of greater than 10 μΜ for each enzyme; and "*(X μΜ)" indicates that no inhibition was observed at the highest concentration (i.e., X μΜ) of compound tested.
Table 2. IC50 Values for Compounds of Formula I against EZH2 and Y641N EZH2 Mutant Enzymes.
[00283] EXAMPLE 29. ECsn MEASUREMENTS FOR INHIBITORS IN HELA
CELL ASSAYS.
[00284] H3K27me3 MSD Hela Assay. Trypsinized HeLa cells were counted and diluted in 10% DMEM (Life Technologies, Cat. # 10569) to 5000 cells/75 Seventy-five μΕ of cells were place in each well of a 96-well flat-bottomed plate and incubated at 37°C for 4 hours. Twenty-five μΕ of test compound (at various concentrations) was added to the cells and incubation continued at 37°C for 96 hours. Media was then removed and the cells rinsed once with ice cold PBS. Forty μΕ of ice-cold MSD Buffer AT (10 mM HEPES, pH 7.9, 5 mM MgCl2, 0.25M sucrose, Benzonase (1: 10000), 1% Triton X-100 supplemented with fresh lx Protease Inhibitor cocktail and ImM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF)) was added to each well and the plates placed on ice for 30 minutes. Ten μΕ of 5M NaCl was then added to each well and incubation on ice continued for another 15 minutes. The
material in each well was suspended pipetting up and down and then transferred to a new 96 well plate. The emptied wells were rinsed with 150uL ice-cold 20mM Tris pH 7.5, ImM EDTA, lmM EGTA, supplemented with fresh lx Protease Inhibitor cocktail and ImM AEBSF ("NO salt NO detergent buffer) and transferred to the respective wells in the new plate. Three hundred μΤ of NO Salt NO detergent buffer was then added to each well of lysates and the plates frozen at -80°C.
[00285] On the same day, an appropriate number of MSD standard bind 96-well plates were coated with 3(^L/well of total H3 capture antibody (Millipore, Cat # MAB3422) at 1 μg/mL concentration in PBS. The antibody solution was evenly distributed first by tapping gently on the sides of the plates and then by shaking the plates for a few minutes at 1000 rpm. Antibody coated plates were stored at 4 °C overnight.
[00286] The next day the lysates are thawed to RT. The antibody coated MSD plates are washed 3X with TBS-T (Tris-buffered saline (Fisher Scientific, Cat #BP2471-1) + 0.2% Tween-20). One-hundred fifty μΤ of 5% Blocker A in TBS-T is added to each well. The wells are covered and shaken on a shaker at RT for one hour. The Blocker A step is repeated a second time. After removing the blocker, 25 μΤ of cell lysate is transferred into each antibody coated well. The plates are shaken for 2 hours at RT, the lysate removed and the plates again washed with Blocker A in TBS-T. Twenty-five μΤ of appropriate freshly prepared antibody mix (including both primary and secondary antibodies) is added to each well and the plates shaken for 1 hour at RT. The antibody mix used was one (or both) of those indicated in the table below:
Both H3 antibodies were obtained from Cell Signalling (Cat #s 4499 and 9733). The goat anti -rabbit antibody was obtained from Meso-Scale Discovery (Cat #R32AB-1).
[00287] The antibody mix was then removed and the wells washed with Blocker A. One hundred-fifty μΤ of freshly prepared IX MSD Read Buffer (Meso-Scale Discovery; Cat #R927C-2) was then added to each well and the plates read on a MSD Sector 2400 Plate Reader.
[00288] Data was analyzed using Assay Assistant (Constellation Pharmaceuticals In-house product) and Activity Base (IDBS Ltd, Surrey, UK) template. Data files were imported to Assay
Assistant and assay conditions were specified. A unique Analysis ID was created and the data files exported to Activity Base. An analysis template was created on Activity Base to measure dose-dependent inhibition of H3K27me3 mark and cell viability respectively. Readout of DMSO wells were used to normalize the data. Resulting curves were fitted using Activity base software Model 205 (IDBS Ltd, Surrey, UK). The data was checked for quality, validated and integrated in excel format using SARview (IDBS Ltd, Surrey, UK).
[00289] H3K27me3 Alpha Hela Assay (AlphaLISA). Ten different doses of each test compound (in a series of 3-fold dilutions) were plated in duplicate 384-well tissue culture treated plates (Catalog # 781080; Greiner Bio One; Monroe, North Carolina). Hela cells grown in culture were trypsinized and counted using a Countess® cell counter (Catalog # CI 0281 ; Life Technologies, Grand Island, NY). Cell were diluted to 67,000 cells per mL inl0% DMEM (Catalog # 10569-010 Life Technologies, Grand Island, NY) and 15μί (1,000 cells) were plated into each well using the Biotek MicroFlo™ Select Dispenser (BioTek Instruments, Inc. Vermont, USA),) of the 384-well plate. Plates were incubated at 37°C 15% C02 for 72 hrs. One of the duplicate plates was processed for HeLa assay and the other for viability.
[00290] To the plate processed for AlphaLISA was added 5μL· per well Cell-Histone Lysis buffer (IX) (Catalog # AL009F1 Perkin Elmer; Waltham, MA) and the plate was incubated at RT for 30 minutes on a plate shaker with low speed (Model# 4625 -Q Thermo Scientific; Waltham, MA). Then, ΙΟμί per well Histone Extraction buffer (catalog # AL009F2; Perkin Elmer; Waltham, MA) was added and the plate further incubated at RT for 20 min on plate shaker with low speed. To each well was then added l0μL· per well of a 5X mix of anti-K27me3 acceptor beads plus Biotinylated anti-Histone H3 (C-ter) Antibody (diluted to 3nM final) (Catalog #AL118 Perkin Elmer; Waltham, MA). Dilution of the acceptor beads and then anti-Histone H3 was with IX Histone Detection buffer (Catalog # AL009F3 Perkin Elmer; Waltham, MA) which was produced diluted from the 1 OX stock provided. The plate was sealed with an aluminum plate sealer and incubated at 23 °C for 60 min. We then added l0μL· 5X solution of Streptavidin Donor beads (Catalog #6760002 Perkin Elmer; Waltham, MA) (20μg/mL final in IX Histone Detection Buffer), sealed the plate with Aluminum plate sealer and incubated at 23 °C for 30 min. The plates were then read using an En Vision- Alpha Reader (model # 2104 Perkin Elmer; Waltham, MA).
[00291] Cell viability was assayed by adding 15 μΐ. of Cell Titer Glo ((Catalog #G7571 Promega Madison, WI) to each well with cells with media. The plates were incubated for RT for 15 - 20 minutes on a plate shaker at low speed. The plates were then read using an EnVision- Alpha Reader (model # 2104 Perkin Elmer; Waltham, MA).
[00292] Data from both assays was analyzed using Assay Assistant (Constellation Pharmaceuticals In-house product) and Activity Base (IDBS Ltd, Surrey, UK) template. Data files were imported to Assay Assistant and assay conditions were specified. A unique Analysis ID was created and the data files exported to Activity Base. An analysis template was created on Activity Base to measure dose-dependent inhibition of H3K27me3 mark and cell viability respectively. Readout of DMSO wells were used to normalize the data. Resulting curves were fitted using Activity base software Model 205 (IDBS Ltd, Surrey, UK). The data was checked for quality, validated and integrated in excel format using SARview (IDBS Ltd, Surrey, UK).
[00293] Table 3 shows the activity of selected compounds of this invention in the two different HeLa cell assays described above. EC50 values are reported as follows: "A" indicates an EC50 value of less than 400 nM; "B" indicates an EC50 value of 400 nM to 2 μΜ; "C" indicates an EC50 value of greater than 2 μΜ and less than 10 μΜ for each enzyme; "D" indicates an EC50 value of greater than 10 μΜ for each enzyme; and "*(X μΜ)" indicates that no inhibition was observed at the highest concentration (i.e., X μΜ) of compound tested.
Table 3. EC50 Values for Tested Compounds of the Invention in Hela Cells Expressing H3k27 Wild Type EZH2.
Compound HeLa HeLa_ Compound HeLa HeLa_ No. (EC50) (EC50) No. (EC50) (EC50)
133 A 140 A
134 B 141 A
135 A 142 A
136 A 143 A
137 A 144 A
138 A 145 A
139 A
[00294] Karpas-422 GI50 Assay - Ten different doses of each test compound (in a series of 3-fold dilutions) are plated in 384-well tissue culture treated plates (Catalog # 781080; Greiner Bio One; Monroe, North Carolina). Karpas-422 cells grown in suspension culture are counted using a Countess® cell counter (Catalog # C10281 ; Life Technologies, Grand Island, NY). Cells are diluted to 25,000 cells per mL in 10% RPMI (Catalog # 61870-036 Life Technologies, Grand Island, NY) and 45μL· (1 ,125 cells) are plated into each well using the Biotek MicroFlo™ Select Dispenser (BioTek Instruments, Inc. Vermont, USA),) of the 384-well plate. Plates were incubated at 37°C 15% C02 for 168 hrs (7 days).
[00295] Cell viability is assayed by adding 30 of Cell Titer Glo ((Catalog #G7571 Promega Madison, WI) to each well with cells with media. The plates are incubated at RT for 30 minutes on a plate shaker at low speed. The plates are then read using an En Vision-Alpha Reader (model # 2104 Perkin Elmer; Waltham, MA).
[00296] Data from the assay is analyzed using Assay Assistant (Constellation Pharmaceuticals In-house product) and Activity Base (IDBS Ltd, Surrey, UK) template. Data files are imported to Assay Assistant and assay conditions are specified. A unique Analysis ID is created and the data files exported to Activity Base. An analysis template is created on Activity Base to measure dose-dependent cell viability. Readout of DMSO wells are used to normalize the data. Resulting curves are fitted using Activity base software Model 205 (IDBS Ltd, Surrey, UK). The data is checked for quality, validated and integrated in excel format using SARview (IDBS Ltd, Surrey, UK).
Claims
We claim:
(I), or a pharmaceutically acceptable salt or tautomer thereof, wherein:
Z is C(R9) or N;
1 2
one of Y or Y is N and the other is C;
one of X1, X2, X3, or X4 is N and each of the others is independently C(R5);
1 2 9
each of R , R and R is independently selected from hydrogen, halo, -OH, -CN, C1-C4 alkyl, -0-(Ci-C4 alkyl), -N(R7)2, -(C0-C4 alkylene)-aryl, -(C0-C4 alkylene)-heteroaryl, -(C0-C4 alkylene)-heterocyclyl, and -(Co-C4 alkylene)-carbocyclyl; or
R and Ry are taken together with atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring; or
2 9
R" and R are taken together with atoms to which they are bound to form an aryl, heteroaryl, heterocyclyl, or carbocyclyl ring;
each of R3, R4 and R5 is independently selected from hydrogen, halo, -CN, -(Co-C4 alkylene)-R8, -(C2-C6 alkenylene or alkynylene)-R6, -(Ci-C4 alkylene)-0-R6, -(Ci-C4
alkylene)-0-(Ci-C4 alkylene)-R8, -O-(C0-C4 alkylene)-R6, -0-(C2-C4 alkylene)-0-R8, -0-(Ci-C4 alkylene)-R6, -(C0-C4 alkylene)-N(R7)2, -(C0-C4 alkylene)-C(0)-0-R6, -(C0-C4
alkylene)-0-C(0)-R6, -(C0-C4 alkylene)-C(0)-N(R7)2, -(C0-C4 alkylene)-N(R7)-C(0)-R6, -O- (Ci-C4 alkylene)-C(0)-N(R7)2, -0-(C2-C4 alkylene)-N(R7)-C(0)-(R7), -(C0-C4
alkylene)-S(0)-R8, -(C0-C4 alkylene)-S(0)2-R8, -(C0-C4 alkylene)-S(0)2-N(R7)2, -(C0-C4 alkylene)-N(R7)-S(0)2-R8, and -(C0-C4 alkylene)-C(0)-R8;
each R6 is independently selected from hydrogen or R8;
each R7 is independently selected from hydrogen, -(Co-C4 alkylene)-R6, -(Co-C4 alkylene)-0-R6, -S(0)2-R8, -C(=0)-R8, -C(=0)-N(R6)2, -(Ci-C4 alkylene)-0-C(=0)-R8 and -(C0- C4 alkylene)-C(=0)-0-R6; or
two R are taken together with the nitrogen atom to which they are commonly bound to form an optionally substituted heterocyclyl or heteroaryl;
Q
R is selected from C1-C4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl;
wherein unless otherwise designated any alkyl, alkylene, alkenyl, alkynyl, aryl, heteroaryl, heterocyclyl or carbocyclyl portion of the compound is optionally substituted.
2. The compound of claim 1, wherein Z is CH.
3. The compound of claim 1 or 2, wherein each of R 1 and R 2 is independently selected from -C1-C3 alkyl, -Q-C3 haloalkyl, 0-(d-C3 alkyl) and -0-(d-C3 haloafkyl).
4. The compound of claim 3, wherein R1 is methyl.
5. The compound of claim 3 or 4, wherein R is selected
from -CH3, -CH2CH3, -CH2CH2CH3, -OCH3, and -OCHF2.
6. The compound of any one of claims 1-5, wherein Y1 is N and X4 is N.
7. The compound of claim 6, wherein X5 is selected from C(H), C(OCH3) and C(CH3).
8. The compound of any one of claims 1-5, wherein Y 1 is N and X 2 is N.
9. The compound of any one of claims 1-5, wherein Y 2 is N and X 3 is N.
10. The compound of any one of claims 1-9, wherein R is methyl or chloro.
11. The compound of any one of claims 1-10, wherein R4 is selected
from -heteroaryl, -CH(CH3)-heterocyclyl, -CH(CH3)-heteroaryl, -CH(CH3)-aryl, -CH(CH3)-carb ocyclyl, -CH(CH3)-N(R10)-S(O)2-(Ci-C4 alkyl), -CH(CH3)-N(R10)-C(O)-(Ci-C4 afkyl), and -CH(CH3)N(R10)2, wherein R10 is selected from hydrogen and Ci-C4 alkyl, and wherein any
aryl, heteroaryl, heterocyclyl or carbocyclyl portion of R is optionally substituted.
12. The compound of claim 11, wherein R4 is selected from l-(l-methylpiperidin-4-yl)ethyl, 5-methyl-isoxazol-4-yl, 3,5-dimethyl-isoxazol-4-yl, l,5-dimethyl-lH-pyrazol-3-yl, 1-(1- ethylsulfonylpiperidin-4-yl)ethyl, l,4-dimethyl-lH-pyrazol-5-yl, 1 -methyl- lH-pyrazol-5-yl, 1- (tetrahydropyran-4-yl)ethyl, 1 -(pyridin-3-yl)ethyl, 1 -(methylsulfonylamino)ethyl, 1 -(1 -methyl-2- oxopiperidin-4-yl)ethyl, 1 -(methylsulf onyl(N-ethyl)amino)ethyl, 1 -(methylsulfonyl(N- methyl)amino)ethyl, 1 -phenylethyl, l-(methylcarbonyl(N-methyl)amino)ethyl, and 1- cyclopropylethyl.
13. The compound of claim 11, wherein R4 is selected from l-(l-(2,2- difluoropropanoyl)piperidin-4-yl)ethyl, 1 -(1 -(2,2-difluoroethanoyl)piperidin-4-yl)ethyl, 1 -(1- (2,2,2-trifluoroethyl)piperidin-4-yl)ethyl, l-(l-(2,2,2-trifluoroethanoyl)piperidin-4-yl)ethyl, 1-(1- (2-fluoro-2-methylpropyl)piperidin-4-yl)ethyl, 1 -( 1 -(2,2-difluoropropyl)piperidin-4-yl)ethyl, 1 -
( 1 -(3 ,3 ,3 -trifluoropropanoyl)piperidin-4-yl)ethyl, 1 -( 1 -(3 , 3 ,3 -trifluoropropyl)piperidin-4- yl)ethyl, l-(l-(2-hydroxy-2-methylpropyl)piperidin-4-yl)ethyl, l-(l-(2,2-difluoroethyl)piperidin- 4-yl)ethyl, 1 -(dimethylamino)ethyl, morpholine-4-carbonyl, 4-methylsulfonylpiperazin-l- ylcarbonyl, 1 -(tetrahydro-2H-pyran-4-yl)ethyl, 1 -( 1 -(2-fluoro-2-methylpropanoyl)piperidin-4- yl)ethyl, and 1 -(ethyl(methyl)amino)ethyl.
14. The compound of any one of claims 1-13, wherein each R5 is hydrogen.
The compound of claim 1 , wherein the compound is of the structural formula (II)
(II); or a pharmaceutically acceptable salt or tautomer thereof, wherein:
each of R 1 , R2 and R 9 is independently selected from hydrogen, C1-C4 alkyl, and -0-(Ci- C4 alkyl);
one of Y 1 or Y 2 is N and the other is C;
one of X 2 , X 3 , or X 4 is N and each of the others is independently CH or -0-(Ci-C4 alkyl); R is selected from hydrogen, halo, and Ci-C4 alkyl;
R4 is selected from -C(0)-R8, -(C0-C4 alkylene)-R8, and -(C0-C4 alkylene)-N(R7)2;
η
R is selected from hydrogen, Ci-C4 alkyl, -S(0)2-Ci-C4 alkyl, and -C(=0)-Ci-C4 alkyl; or
η
two R are taken together with the nitrogen atom to which they are commonly bound to form an optionally substituted heterocyclyl; and
R is selected from Ci-C4 alkyl, aryl, heteroaryl, carbocyclyl and heterocyclyl, wherein each of the aryl, heteroaryl, carbocyclyl and heterocyclyl are optionally substituted.
16. The compound of any one of claims 1-15, selected from any one of compounds 100-145.
17. A composition comprising a compound of any one of claims 1-16; and a
pharmaceutically acceptable carrier.
18. The composition of claim 17 for use in treating cancer in a patient.
19. The composition of claim 17 for use in modulating the activity of an EZH1 protein in a patient.
20. The composition of claim 17 for use in modulating the activity of an EZH2 protein in a patient.
21. The composition of claim 20, wherein EZH2 protein to be modulated is a mutant EZH2 protein.
22. The composition of claim 21, wherein the mutant EZH2 protein comprises a Y641N, Y641C, Y641F, Y641H, Y641S, A677G, or A687V mutation.
23. The composition of claim 22, wherein the mutant EZH2 protein comprises a Y641N mutation.
24. The composition of any one of claims 16-20, wherein the patient is suffering from cancer.
25. The composition of claim 24, wherein the cancer is characterized by trimethylation of histone H3 at lysine 27.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14723919.8A EP2970305B1 (en) | 2013-03-15 | 2014-03-12 | Modulators of methyl modifying enzymes, compositions and uses thereof |
US14/769,471 US9745305B2 (en) | 2013-03-15 | 2014-03-12 | Modulators of methyl modifying enzymes, compositions and uses thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361790566P | 2013-03-15 | 2013-03-15 | |
US61/790,566 | 2013-03-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2014151142A1 true WO2014151142A1 (en) | 2014-09-25 |
Family
ID=50721878
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2014/025081 WO2014151142A1 (en) | 2013-03-15 | 2014-03-12 | Modulators of methyl modifying enzymes, compositions and uses thereof |
Country Status (3)
Country | Link |
---|---|
US (1) | US9745305B2 (en) |
EP (1) | EP2970305B1 (en) |
WO (1) | WO2014151142A1 (en) |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016507573A (en) * | 2012-02-10 | 2016-03-10 | コンステレーション・ファーマシューティカルズ・インコーポレイテッドConstellation Pharmaceuticals,Inc. | Methyl group-modifying enzyme regulator, composition and use thereof |
WO2016102493A1 (en) * | 2014-12-22 | 2016-06-30 | Bayer Pharma Aktiengesellschaft | Imidazopyridine ezh2 inhibitors |
WO2017018975A1 (en) | 2015-07-24 | 2017-02-02 | Constellation Pharmaceuticals, Inc. | Combination therapies for modulation of histone methyl modifying enzymes |
WO2017023671A1 (en) | 2015-08-03 | 2017-02-09 | Constellation Pharmaceuticals, Inc. | Ezh2 inhibitors and modulation of regulatory t-cell function |
WO2017025493A1 (en) * | 2015-08-12 | 2017-02-16 | Bayer Pharma Aktiengesellschaft | Quinoline ezh2 inhibitors |
US9718838B2 (en) | 2015-08-27 | 2017-08-01 | Eli Lilly And Company | Inhibitors of EZH2 |
US9969716B2 (en) | 2013-08-15 | 2018-05-15 | Constellation Pharmaceuticals, Inc. | Indole derivatives as modulators of methyl modifying enzymes, compositions and uses thereof |
WO2019014191A1 (en) | 2017-07-10 | 2019-01-17 | Constellation Pharmaceuticals, Inc. | Ezh2 inhibitor-induced gene expression |
WO2019011217A1 (en) * | 2017-07-10 | 2019-01-17 | 中国科学院上海药物研究所 | Pyrrolo[1,2-b]pyridazine compounds or pharmacologically acceptable salts thereof and use thereof |
US10220036B2 (en) | 2014-12-23 | 2019-03-05 | Novartis Ag | Triazolopyrimidine compounds and uses thereof |
US10266542B2 (en) | 2017-03-15 | 2019-04-23 | Mirati Therapeutics, Inc. | EZH2 inhibitors |
CN109790166A (en) * | 2016-06-20 | 2019-05-21 | 诺华股份有限公司 | Imidazopyridine is used for treating cancer |
WO2019133674A1 (en) | 2017-12-28 | 2019-07-04 | Constellation Pharmaceuticals, Inc. | Pharmacokinetic enhancement of ezh2 inhibitors through combination therapies |
CN110117251A (en) * | 2018-02-06 | 2019-08-13 | 上海睿升化工科技有限公司 | The preparation method of the chloro- 2 hydroxy pyrimidine of 4,5- bis- |
WO2019178191A1 (en) | 2018-03-14 | 2019-09-19 | Biogen Ma Inc. | O-glycoprotein-2-acetamido-2-deoxy-3-d-glycopyranosidase inhibitors |
US10457640B2 (en) | 2016-10-19 | 2019-10-29 | Constellation Pharmaceuticals, Inc. | Synthesis of inhibitors of EZH2 |
WO2020011607A1 (en) | 2018-07-09 | 2020-01-16 | Fondation Asile Des Aveugles | Inhibition of prc2 subunits to treat eye disorders |
US10577350B2 (en) | 2015-08-28 | 2020-03-03 | Constellation Pharmaceuticals, Inc. | Crystalline forms of (R)-N-((4-methoxy-6-methyl-2-oxo-1,2-dihydropyridin-3-yl)methyl)-2-methyl-1-(1-(1-(2,2,2-trifluoroethyl)piperidin-4-yl)ethyl)-1H-indole-3-carboxamide |
WO2020061150A1 (en) | 2018-09-19 | 2020-03-26 | Biogen Ma Inc. | O-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
WO2020117961A1 (en) | 2018-12-05 | 2020-06-11 | Biogen Ma Inc. | Morpholinyl, piperazinyl, oxazepanyl and diazepanyl o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
US10689378B2 (en) | 2016-06-20 | 2020-06-23 | Novartis Ag | Triazolopyridine compounds and uses thereof |
WO2020139339A1 (en) | 2018-12-27 | 2020-07-02 | Constellation Pharmaceuticals, Inc. | Ezh2 and androgen receptor signaling inhibitors as tools for targeting prostate cancer |
WO2020163193A1 (en) | 2019-02-04 | 2020-08-13 | Biogen Ma Inc. | Bicyclic ether o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
WO2020185593A1 (en) | 2019-03-08 | 2020-09-17 | Biogen Ma Inc. | Azetidinyl 0-glyc0pr0tein-2-acetamid0-2-deoxy-3-d-gluc0pyran0sidase inhibitors |
WO2021035194A1 (en) | 2019-08-22 | 2021-02-25 | Juno Therapeutics, Inc. | Combination therapy of a t cell therapy and an enhancer of zeste homolog 2 (ezh2) inhibitor and related methods |
WO2021086966A1 (en) | 2019-10-29 | 2021-05-06 | Biogen Ma Inc. | Spirocyclic o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
US11091489B2 (en) | 2016-06-20 | 2021-08-17 | Novartis Ag | Crystalline forms of a triazolopyrimidine compound |
US11091495B2 (en) | 2018-01-31 | 2021-08-17 | Mirati Therapeutics, Inc. | Substituted imidazo[1,2-c]pyrimidines as PRC2 inhibitors |
WO2022031701A1 (en) | 2020-08-03 | 2022-02-10 | Biogen Ma Inc. | Crystaline forms of an o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitor |
EP4026830A1 (en) | 2021-01-12 | 2022-07-13 | Westlake University | Protease inhibitors, preparation, and uses thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011140324A1 (en) * | 2010-05-07 | 2011-11-10 | Glaxosmithkline Llc | Indoles |
WO2012118812A2 (en) * | 2011-02-28 | 2012-09-07 | Epizyme, Inc. | Substituted 6,5-fused bicyclic heteroaryl compounds |
WO2013120104A2 (en) * | 2012-02-10 | 2013-08-15 | Constellation Pharmaceuticals | Modulators of methyl modifying enzymes, compositions and uses thereof |
Family Cites Families (63)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE52670B1 (en) | 1981-03-03 | 1988-01-20 | Leo Ab | Heterocyclic carboxamides,compositions containing such compounds,and processes for their preparation |
US5308854A (en) | 1990-06-18 | 1994-05-03 | Merck & Co., Inc. | Inhibitors of HIV reverse transcriptase |
KR100537843B1 (en) | 1996-07-22 | 2006-04-28 | 다이이치 아스비오파마 가부시키가이샤 | Arylpiperidinol and arylpiperidine derivatives and pharmaceuticals containing them |
CA2458699C (en) | 2001-09-04 | 2010-10-19 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | Novel dihydropteridinones, method for producing the same and the use thereof as medicaments |
US6897220B2 (en) | 2001-09-14 | 2005-05-24 | Methylgene, Inc. | Inhibitors of histone deacetylase |
MXPA03000145A (en) | 2002-01-07 | 2003-07-15 | Pfizer | Oxo or oxy-pyridine compounds as 5-ht4 receptor modulators. |
WO2003079986A2 (en) | 2002-03-18 | 2003-10-02 | Bristol-Myers Squibb Company | Uracil derivatives as inhibitors of tnf-alpha converting enzyme (tace) and matrix metalloproteinases |
TWI319387B (en) | 2002-04-05 | 2010-01-11 | Astrazeneca Ab | Benzamide derivatives |
TW200500341A (en) | 2002-11-12 | 2005-01-01 | Astrazeneca Ab | Novel compounds |
CN101014571A (en) | 2004-01-30 | 2007-08-08 | 默克公司 | N-benzyl-3,4-dihyroxypyridine-2-carboxamide and n-benzyl-2,3-dihydroxypyridine-4- carboxamide compounds useful as HIV integrase inhibitors |
MXPA06012333A (en) | 2004-04-30 | 2007-01-17 | Takeda Pharmaceutical | Heterocyclic amide compound and use thereof as an mmp-13 inhibitor. |
EP1765994B1 (en) | 2004-06-01 | 2009-11-18 | The University of North Carolina at Chapel Hill | Reconstituted histone methyltransferase complex and methods of identifying modulators thereof |
US7732475B2 (en) | 2005-07-14 | 2010-06-08 | Takeda San Diego, Inc. | Histone deacetylase inhibitors |
JP2009503014A (en) | 2005-08-03 | 2009-01-29 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | Dihydropteridinone in the treatment of respiratory diseases |
KR20080080525A (en) | 2005-12-08 | 2008-09-04 | 노파르티스 아게 | Effects of inhibitors of fgfr3 on gene transcription |
US8053435B2 (en) | 2006-11-03 | 2011-11-08 | Korea Research Institute Of Chemical Technology | Naphthalenyloxypropenyl derivatives having inhibitory activity against histone deacetylase and pharmaceutical composition comprising the same |
WO2008089397A2 (en) | 2007-01-19 | 2008-07-24 | The Regents Of The University Of Michigan | Adrb2 cancer markers |
MX2009008531A (en) | 2007-02-16 | 2009-08-26 | Amgen Inc | Nitrogen-containing heterocyclyl ketones and methods of use. |
US8030344B2 (en) | 2007-03-13 | 2011-10-04 | Methylgene Inc. | Inhibitors of histone deacetylase |
WO2009006577A2 (en) | 2007-07-03 | 2009-01-08 | The Regents Of The University Of Michigan | Compositions and methods for inhibiting ezh2 |
WO2009015203A1 (en) | 2007-07-23 | 2009-01-29 | Syndax Pharmaceuticals, Inc. | Novel compounds and methods of using them |
FR2922209B1 (en) | 2007-10-12 | 2010-06-11 | Sanofi Aventis | 5,6-DIARYLES PYRIDINES SUBSTITUTED IN POSITION 2 AND 3, THEIR PREPARATION AND THEIR USE IN THERAPEUTICS. |
JP2011506333A (en) | 2007-12-06 | 2011-03-03 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニー | Novel sEH inhibitors and uses thereof |
UY31736A (en) | 2008-03-26 | 2009-11-10 | Takeda Pharmaceutical | SUBSTITUTED DERIVATIVES OF PIRAZOL AND ITS USE |
JP2011524894A (en) | 2008-06-18 | 2011-09-08 | ファイザー・リミテッド | Nicotinamide derivatives |
US8389720B2 (en) | 2008-11-13 | 2013-03-05 | Merck Sharp & Dohme Corp. | Quinolone neuropeptide S receptor antagonists |
WO2011055270A1 (en) | 2009-11-04 | 2011-05-12 | Wyeth Llc | Indole based receptor crth2 antagonists |
US8575203B2 (en) | 2010-04-21 | 2013-11-05 | Boehringer Ingelheim International Gmbh | Chemical compounds |
WO2012005805A1 (en) | 2010-05-07 | 2012-01-12 | Glaxosmithkline Llc | Azaindazoles |
JP5864546B2 (en) | 2010-05-07 | 2016-02-17 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニーGlaxoSmithKline LLC | Indazole |
WO2012024543A1 (en) | 2010-08-18 | 2012-02-23 | Caris Life Sciences Luxembourg Holdings | Circulating biomarkers for disease |
US9175331B2 (en) | 2010-09-10 | 2015-11-03 | Epizyme, Inc. | Inhibitors of human EZH2, and methods of use thereof |
DK2614369T3 (en) | 2010-09-10 | 2016-05-02 | Epizyme Inc | METHOD FOR DETERMINING THE SUITABILITY OF HUMAN EZH2 INHIBITORS TREATED |
WO2012051492A2 (en) | 2010-10-14 | 2012-04-19 | University Of Georgia Research Foundation, Inc. | Compounds and methods for inhibiting hiv latency |
US20130310379A1 (en) | 2010-11-19 | 2013-11-21 | Constellation Pharmaceuticals | Modulators of methyl modifying enzymes, compositions and uses thereof |
EP2646020B1 (en) | 2010-12-01 | 2016-09-21 | Glaxosmithkline LLC | Indoles |
US20140141986A1 (en) | 2011-02-22 | 2014-05-22 | David Spetzler | Circulating biomarkers |
JO3438B1 (en) | 2011-04-13 | 2019-10-20 | Epizyme Inc | Aryl- or heteroaryl-substituted benzene compounds |
TW201733984A (en) | 2011-04-13 | 2017-10-01 | 雅酶股份有限公司 | Substituted benzene compounds |
WO2013039988A1 (en) | 2011-09-13 | 2013-03-21 | Glax0Smithkline Llc | Azaindazoles |
US9730925B2 (en) | 2011-09-30 | 2017-08-15 | Glaxomithkline Llc | Methods of treating cancer |
WO2013067302A1 (en) | 2011-11-04 | 2013-05-10 | Glaxosmithkline Intellectual Property (No. 2) Limited | Method of treatment |
JP6005753B2 (en) | 2011-11-04 | 2016-10-12 | グラクソスミスクライン、インテレクチュアル、プロパティー、(ナンバー2)、リミテッドGlaxosmithkline Intellectual Property (No.2) Limited | Method of treatment |
WO2013067300A1 (en) | 2011-11-04 | 2013-05-10 | Glaxosmithkline Intellectual Property (No. 2) Limited | Method of treatment |
US9051269B2 (en) | 2011-11-18 | 2015-06-09 | Constellation Pharmaceuticals, Inc. | Modulators of methyl modifying enzymes, compositions and uses thereof |
EP2780014A4 (en) | 2011-11-18 | 2015-07-01 | Constellation Pharmaceuticals Inc | Modulators of methyl modifying enzymes, compositions and uses thereof |
WO2013078320A1 (en) | 2011-11-21 | 2013-05-30 | Constellation Pharmaceuticals | Modulators of methyl modifying enzymes, compositions and uses thereof |
US20130230511A1 (en) | 2012-02-03 | 2013-09-05 | Board Of Regents, The University Of Texas System | Biomarkers for response to tyrosine kinase pathway inhibitors in cancer |
CN110420327B (en) | 2012-03-12 | 2022-05-03 | Epizyme股份有限公司 | Human EZH2 inhibitors and methods of use thereof |
SG10201912111TA (en) | 2012-04-13 | 2020-02-27 | Epizyme Inc | Combination therapy for treating cancer |
ES2745016T3 (en) | 2012-04-13 | 2020-02-27 | Epizyme Inc | N - ((4,6-Dimethyl-2-oxo-L, 2-dihydropyridin-3-yl) methyl) -5- (ethyl (tetrahydro-2H-pyran-4-yl) amino) -4-methyl hydrobromide -4 '- (morpholinomethyl) - [L, 1'-biphenyl] -3-carboxamide for use in the treatment of a cell proliferative disorder of the hematological system |
US9562041B2 (en) | 2012-05-16 | 2017-02-07 | Glaxosmithkline Llc | Enhancer of zeste homolog 2 inhibitors |
CA2884848C (en) | 2012-09-28 | 2017-08-22 | Pfizer Inc. | Benzamide and heterobenzamide compounds |
CN105142642B (en) | 2012-10-15 | 2019-10-18 | Epizyme股份有限公司 | Cancer treatment method |
EP2906538A4 (en) | 2012-10-15 | 2016-05-11 | Epizyme Inc | Substituted benzene compounds |
TR201812261T4 (en) | 2012-11-01 | 2018-11-21 | Infinity Pharmaceuticals Inc | CANCER TREATMENT WITH PI3 KINASE ISOFORM MODULATORS |
US9889180B2 (en) | 2012-11-19 | 2018-02-13 | Agency For Science, Technology And Research | Method of treating cancer |
WO2014085666A1 (en) | 2012-11-27 | 2014-06-05 | Board Of Regents, The University Of Texas System | Methods of characterizing and treating molecular subset of muscle-invasive bladder cancer |
US9895390B2 (en) | 2012-12-10 | 2018-02-20 | Children's Medical Center Corporation | Methods and assays for combination treatment of cancer |
WO2014100080A1 (en) | 2012-12-19 | 2014-06-26 | Glaxosmithkline Llc | Combination |
UA111305C2 (en) | 2012-12-21 | 2016-04-11 | Пфайзер Інк. | Condensed with lactams of aryl and heteroaryl |
US9374093B2 (en) | 2014-01-10 | 2016-06-21 | Freescale Semiconductor, Inc. | Capacitively coupled input buffer |
WO2015200650A1 (en) | 2014-06-25 | 2015-12-30 | Epizyme, Inc. | Substituted benzene and 6,5-fused bicyclic heteroaryl compounds |
-
2014
- 2014-03-12 EP EP14723919.8A patent/EP2970305B1/en active Active
- 2014-03-12 US US14/769,471 patent/US9745305B2/en active Active
- 2014-03-12 WO PCT/US2014/025081 patent/WO2014151142A1/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011140324A1 (en) * | 2010-05-07 | 2011-11-10 | Glaxosmithkline Llc | Indoles |
WO2012118812A2 (en) * | 2011-02-28 | 2012-09-07 | Epizyme, Inc. | Substituted 6,5-fused bicyclic heteroaryl compounds |
WO2013120104A2 (en) * | 2012-02-10 | 2013-08-15 | Constellation Pharmaceuticals | Modulators of methyl modifying enzymes, compositions and uses thereof |
Non-Patent Citations (19)
Title |
---|
CAO; ZHANG, MOL. CELL, vol. 15, 2004, pages 57 - 67 |
CARRUTHERS: "Some Modern Methods of Organic Synthesis", 1987, CAMBRIDGE UNIVERSITY PRESS |
ELIEL, E.L.: "Stereochemistry of Carbon Compounds", 1962, MCGRAW-HILL |
ERNST ET AL., NAT GENET., vol. 42, no. 8, August 2010 (2010-08-01), pages 722 - 6 |
JACQUES ET AL.: "Enantiomers, Racemates and Resolutions", 1981, WILEY INTERSCIENCE |
KLEER ET AL., PROC. NAT. ACAD. SCI. USA, vol. 100, 2003, pages 11606 - 11611 |
LAROCK: "Comprehensive Organic Transformations", 1989, VCH PUBLISHERS, INC. |
LUGER ET AL., NATURE, vol. 389, 1997, pages 251 - 260 |
MARGUERON ET AL., CURR. OPIN. GENET. DEV., vol. 15, 2005, pages 163 - 176 |
MIN ET AL.: "An oncogene-tumor suppressor cascade drives metastatic prostate cancer by coordinately activating Ras and nuclear factor- kappaB", NAT MED., vol. 16, no. 3, March 2010 (2010-03-01), pages 286 - 94 |
NIKOLOSKI ET AL., NAT GENET., vol. 42, no. 8, August 2010 (2010-08-01), pages 665 - 7 |
SHARAD K. VERMA ET AL: "Identification of Potent, Selective, Cell-Active Inhibitors of the Histone Lysine Methyltransferase EZH2", ACS MEDICINAL CHEMISTRY LETTERS, vol. 3, no. 12, 13 December 2012 (2012-12-13), pages 1091 - 1096, XP055106955, ISSN: 1948-5875, DOI: 10.1021/ml3003346 * |
SMITH; MARCH: "March's Advanced Organic Chemistry", 2001, JOHN WILEY & SONS, INC. |
SNEERINGER ET AL.: "Proceedings of the National Academy of Sciences, PNAS", 15 November 2010, article "Coordinated activities of wild-type plus mutant EZH2 drive tumor-associated hypertrimethylation of lysine 27 on histone H3 (H3K27) in human B-cell lymphomas" |
THOMAS SORRELL: "Organic Chemistry", 1999, UNIVERSITY SCIENCE BOOKS |
VARAMBALLY ET AL.: "The polycomb group protein EZH2 is involved in progression of prostate cancer", NATURE, vol. 419, 2002, pages 624 - 629, XP002969193, DOI: doi:10.1038/nature01075 |
WILEN ET AL., TETRAHEDRON, vol. 33, 1977, pages 2725 |
WILEN, S.H.: "Tables of Resolving Agents and Optical Resolutions", 1972, UNIV. OF NOTRE DAME PRESS, pages: 268 |
YU ET AL.: "An Integrated Network of Androgen Receptor, Polycomb, and TMPRSS2-ERG Gene Fusions in Prostate Cancer Progression", CANCER CELL, vol. 17, no. 5, 18 May 2010 (2010-05-18), pages 443 - 454, XP055070277, DOI: doi:10.1016/j.ccr.2010.03.018 |
Cited By (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016507573A (en) * | 2012-02-10 | 2016-03-10 | コンステレーション・ファーマシューティカルズ・インコーポレイテッドConstellation Pharmaceuticals,Inc. | Methyl group-modifying enzyme regulator, composition and use thereof |
US10016405B2 (en) | 2012-02-10 | 2018-07-10 | Constellation Pharmaceuticals, Inc. | Modulators of methyl modifying enzymes, compositions and uses thereof |
US9969716B2 (en) | 2013-08-15 | 2018-05-15 | Constellation Pharmaceuticals, Inc. | Indole derivatives as modulators of methyl modifying enzymes, compositions and uses thereof |
WO2016102493A1 (en) * | 2014-12-22 | 2016-06-30 | Bayer Pharma Aktiengesellschaft | Imidazopyridine ezh2 inhibitors |
US11931363B2 (en) | 2014-12-23 | 2024-03-19 | Novartis Ag | Triazolopyrimidine compounds and uses thereof |
US10220036B2 (en) | 2014-12-23 | 2019-03-05 | Novartis Ag | Triazolopyrimidine compounds and uses thereof |
WO2017018975A1 (en) | 2015-07-24 | 2017-02-02 | Constellation Pharmaceuticals, Inc. | Combination therapies for modulation of histone methyl modifying enzymes |
WO2017023671A1 (en) | 2015-08-03 | 2017-02-09 | Constellation Pharmaceuticals, Inc. | Ezh2 inhibitors and modulation of regulatory t-cell function |
WO2017025493A1 (en) * | 2015-08-12 | 2017-02-16 | Bayer Pharma Aktiengesellschaft | Quinoline ezh2 inhibitors |
US9718838B2 (en) | 2015-08-27 | 2017-08-01 | Eli Lilly And Company | Inhibitors of EZH2 |
US10577350B2 (en) | 2015-08-28 | 2020-03-03 | Constellation Pharmaceuticals, Inc. | Crystalline forms of (R)-N-((4-methoxy-6-methyl-2-oxo-1,2-dihydropyridin-3-yl)methyl)-2-methyl-1-(1-(1-(2,2,2-trifluoroethyl)piperidin-4-yl)ethyl)-1H-indole-3-carboxamide |
US11548897B2 (en) | 2016-06-20 | 2023-01-10 | Novartis Ag | Crystalline forms of a triazolopyrimidine compound |
CN109790166A (en) * | 2016-06-20 | 2019-05-21 | 诺华股份有限公司 | Imidazopyridine is used for treating cancer |
US11091489B2 (en) | 2016-06-20 | 2021-08-17 | Novartis Ag | Crystalline forms of a triazolopyrimidine compound |
US10689378B2 (en) | 2016-06-20 | 2020-06-23 | Novartis Ag | Triazolopyridine compounds and uses thereof |
US10676479B2 (en) | 2016-06-20 | 2020-06-09 | Novartis Ag | Imidazolepyridine compounds and uses thereof |
US10457640B2 (en) | 2016-10-19 | 2019-10-29 | Constellation Pharmaceuticals, Inc. | Synthesis of inhibitors of EZH2 |
US10266542B2 (en) | 2017-03-15 | 2019-04-23 | Mirati Therapeutics, Inc. | EZH2 inhibitors |
CN109232575A (en) * | 2017-07-10 | 2019-01-18 | 中国科学院上海药物研究所 | Pyrroles's [1,2-b] pyridazine compound or its officinal salt and their purposes |
WO2019011217A1 (en) * | 2017-07-10 | 2019-01-17 | 中国科学院上海药物研究所 | Pyrrolo[1,2-b]pyridazine compounds or pharmacologically acceptable salts thereof and use thereof |
CN109232575B (en) * | 2017-07-10 | 2022-01-25 | 中国科学院上海药物研究所 | Pyrrole [1,2-b ] pyridazine compound or pharmaceutically acceptable salt thereof and application thereof |
WO2019014191A1 (en) | 2017-07-10 | 2019-01-17 | Constellation Pharmaceuticals, Inc. | Ezh2 inhibitor-induced gene expression |
WO2019133674A1 (en) | 2017-12-28 | 2019-07-04 | Constellation Pharmaceuticals, Inc. | Pharmacokinetic enhancement of ezh2 inhibitors through combination therapies |
US11485738B2 (en) | 2018-01-31 | 2022-11-01 | Mirati Therapeutics, Inc. | Substituted imidazo[1,2-c]pyrimidines as PRC2 inhibitors |
US11091495B2 (en) | 2018-01-31 | 2021-08-17 | Mirati Therapeutics, Inc. | Substituted imidazo[1,2-c]pyrimidines as PRC2 inhibitors |
US11220509B2 (en) | 2018-01-31 | 2022-01-11 | Mirati Therapeutics, Inc. | Substituted imidazo[1,2-c]pyrimidines as PRC2 inhibitors |
CN110117251A (en) * | 2018-02-06 | 2019-08-13 | 上海睿升化工科技有限公司 | The preparation method of the chloro- 2 hydroxy pyrimidine of 4,5- bis- |
CN110117251B (en) * | 2018-02-06 | 2021-12-10 | 安庆睿升医药科技有限公司 | Preparation method of 4, 5-dichloro-2-hydroxypyridine |
WO2019178191A1 (en) | 2018-03-14 | 2019-09-19 | Biogen Ma Inc. | O-glycoprotein-2-acetamido-2-deoxy-3-d-glycopyranosidase inhibitors |
EP4186899A1 (en) | 2018-03-14 | 2023-05-31 | Biogen MA Inc. | O-glycoprotein-2-acetamido-2-deoxy-3-d-glycopyranosidase inhibitors |
WO2020011607A1 (en) | 2018-07-09 | 2020-01-16 | Fondation Asile Des Aveugles | Inhibition of prc2 subunits to treat eye disorders |
WO2020061150A1 (en) | 2018-09-19 | 2020-03-26 | Biogen Ma Inc. | O-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
WO2020117961A1 (en) | 2018-12-05 | 2020-06-11 | Biogen Ma Inc. | Morpholinyl, piperazinyl, oxazepanyl and diazepanyl o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
EP4327814A2 (en) | 2018-12-05 | 2024-02-28 | Biogen MA Inc. | Morpholinyl, piperazinyl, oxazepanyl and diazepanyl o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
WO2020139339A1 (en) | 2018-12-27 | 2020-07-02 | Constellation Pharmaceuticals, Inc. | Ezh2 and androgen receptor signaling inhibitors as tools for targeting prostate cancer |
WO2020139943A1 (en) | 2018-12-27 | 2020-07-02 | Constellation Pharmaceuticals, Inc. | Ezh2 and androgen receptor signaling inhibitors as tools for targeting prostate cancer |
WO2020163193A1 (en) | 2019-02-04 | 2020-08-13 | Biogen Ma Inc. | Bicyclic ether o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
WO2020185593A1 (en) | 2019-03-08 | 2020-09-17 | Biogen Ma Inc. | Azetidinyl 0-glyc0pr0tein-2-acetamid0-2-deoxy-3-d-gluc0pyran0sidase inhibitors |
WO2021035194A1 (en) | 2019-08-22 | 2021-02-25 | Juno Therapeutics, Inc. | Combination therapy of a t cell therapy and an enhancer of zeste homolog 2 (ezh2) inhibitor and related methods |
WO2021086966A1 (en) | 2019-10-29 | 2021-05-06 | Biogen Ma Inc. | Spirocyclic o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitors |
WO2022031701A1 (en) | 2020-08-03 | 2022-02-10 | Biogen Ma Inc. | Crystaline forms of an o-glycoprotein-2-acetamido-2-deoxy-3-d-glucopyranosidase inhibitor |
EP4026830A1 (en) | 2021-01-12 | 2022-07-13 | Westlake University | Protease inhibitors, preparation, and uses thereof |
Also Published As
Publication number | Publication date |
---|---|
EP2970305A1 (en) | 2016-01-20 |
EP2970305B1 (en) | 2017-02-22 |
US9745305B2 (en) | 2017-08-29 |
US20160009718A1 (en) | 2016-01-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP2970305B1 (en) | Modulators of methyl modifying enzymes, compositions and uses thereof | |
AU2013216721B2 (en) | Modulators of methyl modifying enzymes, compositions and uses thereof | |
US9969716B2 (en) | Indole derivatives as modulators of methyl modifying enzymes, compositions and uses thereof | |
EP2953941B1 (en) | Modulators of methyl modifying enzymes, compositions and uses thereof | |
US9051269B2 (en) | Modulators of methyl modifying enzymes, compositions and uses thereof | |
IL298670A (en) | Inhibitors of kras g12c protein and uses thereof | |
WO2013078320A1 (en) | Modulators of methyl modifying enzymes, compositions and uses thereof | |
EP3768671B1 (en) | Substituted imidazolidin-2-one derivatives as prmt5 inhibitors | |
CN117561058A (en) | CDK2 inhibitors and methods of use thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14723919 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 14769471 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
REEP | Request for entry into the european phase |
Ref document number: 2014723919 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2014723919 Country of ref document: EP |