WO2014143871A3 - Thermoresponsive polymer applications for adherent cell culture and recovery - Google Patents

Thermoresponsive polymer applications for adherent cell culture and recovery Download PDF

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Publication number
WO2014143871A3
WO2014143871A3 PCT/US2014/028027 US2014028027W WO2014143871A3 WO 2014143871 A3 WO2014143871 A3 WO 2014143871A3 US 2014028027 W US2014028027 W US 2014028027W WO 2014143871 A3 WO2014143871 A3 WO 2014143871A3
Authority
WO
WIPO (PCT)
Prior art keywords
scaffolds
methods
recovery
polymers
cell culture
Prior art date
Application number
PCT/US2014/028027
Other languages
French (fr)
Other versions
WO2014143871A2 (en
Inventor
Priya Ramaswami BARANIAK
Vanessa Ragaglia
Original Assignee
Garnet Biotherapeutics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Garnet Biotherapeutics, Inc. filed Critical Garnet Biotherapeutics, Inc.
Publication of WO2014143871A2 publication Critical patent/WO2014143871A2/en
Publication of WO2014143871A3 publication Critical patent/WO2014143871A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides scaffolds, methods of culturing cells on and/or within scaffolds, and methods of making scaffolds, wherein the scaffolding comprises thermoresponsive polymers, e.g., N-isopropylacrylamide (NIPAAm). The scaffolds may include other polymers, cell adhesion factors, polymers that are not NIPAAm, and/or cells. The scaffold also exhibits a phase transition in solution above a lower critical solution temperature (LCST). The scaffolds form structures such as beads and/or threads. The scaffolds can be incorporated into tissue cultures vessels. Also provided are methods for recovering cells cultured on the scaffolds by lowering the temperature below an LCST.
PCT/US2014/028027 2013-03-15 2014-03-14 Thermoresponsive polymer applications for adherent cell culture and recovery WO2014143871A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361793127P 2013-03-15 2013-03-15
US61/793,127 2013-03-15

Publications (2)

Publication Number Publication Date
WO2014143871A2 WO2014143871A2 (en) 2014-09-18
WO2014143871A3 true WO2014143871A3 (en) 2014-10-30

Family

ID=51538278

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/028027 WO2014143871A2 (en) 2013-03-15 2014-03-14 Thermoresponsive polymer applications for adherent cell culture and recovery

Country Status (1)

Country Link
WO (1) WO2014143871A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10695427B2 (en) 2015-04-06 2020-06-30 The Johns Hopkins University Shape memory particles for biomedical uses
GB201702475D0 (en) * 2017-02-15 2017-03-29 Locate Therapeutics Ltd Tissue scaffold and scaffold composition
CN106913904B (en) * 2017-03-06 2020-08-14 苏州大学附属第一医院 Micro-nano tissue engineering scaffold with immunotherapy function and preparation method thereof
GB201718556D0 (en) * 2017-11-09 2017-12-27 Univ Oxford Innovation Ltd Macrocarrier
CN110205243A (en) * 2018-02-28 2019-09-06 中原大学 The manufacturing method of cell attachment device and the cell attachment device
WO2023194813A1 (en) * 2022-04-09 2023-10-12 Hassanpour Fatemeh Thermal- responsive substrate for neural cell culture, processes for producing the same, and methods of using the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070026518A1 (en) * 2005-03-29 2007-02-01 The Regents Of The University Of California Controlling stem cell destiny with tunable matrices
US20090136553A1 (en) * 2007-09-25 2009-05-28 Gerlach Jorg C Triggerably dissolvable hollow fibers for controlled delivery

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070026518A1 (en) * 2005-03-29 2007-02-01 The Regents Of The University Of California Controlling stem cell destiny with tunable matrices
US20090136553A1 (en) * 2007-09-25 2009-05-28 Gerlach Jorg C Triggerably dissolvable hollow fibers for controlled delivery

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YOSHID ET AL.: "Stimuli-Responsive Hydrogels and Their Application to Functional Materials''.", BIOMEDICAL APPLICATIONS OF HYDROGELS HANDBOOK., 2010, NEW YORK, pages 19 - 43 *

Also Published As

Publication number Publication date
WO2014143871A2 (en) 2014-09-18

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