WO2014118685A3 - Method of altering the acidic variant content of antibody - Google Patents

Method of altering the acidic variant content of antibody Download PDF

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Publication number
WO2014118685A3
WO2014118685A3 PCT/IB2014/058553 IB2014058553W WO2014118685A3 WO 2014118685 A3 WO2014118685 A3 WO 2014118685A3 IB 2014058553 W IB2014058553 W IB 2014058553W WO 2014118685 A3 WO2014118685 A3 WO 2014118685A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
altering
variant content
acidic variant
acidic
Prior art date
Application number
PCT/IB2014/058553
Other languages
French (fr)
Other versions
WO2014118685A2 (en
Inventor
Samir R. KULKARNI
Raghavendra YAMMANURU
Original Assignee
Dr. Reddy's Laboratories Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr. Reddy's Laboratories Limited filed Critical Dr. Reddy's Laboratories Limited
Publication of WO2014118685A2 publication Critical patent/WO2014118685A2/en
Publication of WO2014118685A3 publication Critical patent/WO2014118685A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes

Abstract

A method of altering the amount of acidic variants in antibody composition, in particular, employs a cation exchange chromatography with varying amounts of wash buffer column volume to alter the acidic variant content of the antibody.
PCT/IB2014/058553 2013-01-29 2014-01-26 Method of altering the acidic variant content of antibody WO2014118685A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN381/CHE/2013 2013-01-29
IN381CH2013 2013-01-29

Publications (2)

Publication Number Publication Date
WO2014118685A2 WO2014118685A2 (en) 2014-08-07
WO2014118685A3 true WO2014118685A3 (en) 2014-12-31

Family

ID=51263081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2014/058553 WO2014118685A2 (en) 2013-01-29 2014-01-26 Method of altering the acidic variant content of antibody

Country Status (1)

Country Link
WO (1) WO2014118685A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020084503A1 (en) * 2018-10-26 2020-04-30 Cadila Healthcare Limited A composition comprising antibody with reduced level of basic variants thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999057134A1 (en) * 1998-05-06 1999-11-11 Genentech, Inc. Protein purification by ion exchange chromatography
WO2004024866A2 (en) * 2002-09-11 2004-03-25 Genentech, Inc. Protein purification
WO2011150110A1 (en) * 2010-05-25 2011-12-01 Genentech, Inc. Methods of purifying polypeptides
WO2012160530A1 (en) * 2011-05-26 2012-11-29 Dr. Reddy's Laboratories Limited Purification of antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999057134A1 (en) * 1998-05-06 1999-11-11 Genentech, Inc. Protein purification by ion exchange chromatography
WO2004024866A2 (en) * 2002-09-11 2004-03-25 Genentech, Inc. Protein purification
WO2011150110A1 (en) * 2010-05-25 2011-12-01 Genentech, Inc. Methods of purifying polypeptides
WO2012160530A1 (en) * 2011-05-26 2012-11-29 Dr. Reddy's Laboratories Limited Purification of antibodies

Also Published As

Publication number Publication date
WO2014118685A2 (en) 2014-08-07

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