WO2014113846A1 - Orge tolérant les herbicides - Google Patents

Orge tolérant les herbicides Download PDF

Info

Publication number
WO2014113846A1
WO2014113846A1 PCT/AU2014/000060 AU2014000060W WO2014113846A1 WO 2014113846 A1 WO2014113846 A1 WO 2014113846A1 AU 2014000060 W AU2014000060 W AU 2014000060W WO 2014113846 A1 WO2014113846 A1 WO 2014113846A1
Authority
WO
WIPO (PCT)
Prior art keywords
barley
plant
seq
hybrid
anyone
Prior art date
Application number
PCT/AU2014/000060
Other languages
English (en)
Inventor
Jason Konrad EGLINTON
Suong CU
Shannan LARWOOD
Gurjeet GILL
Ben FLEET
Christopher Preston
Peter Boutsalis
Jenna MALONE
Original Assignee
Adelaide Research & Innovation Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2013900274A external-priority patent/AU2013900274A0/en
Application filed by Adelaide Research & Innovation Pty Ltd filed Critical Adelaide Research & Innovation Pty Ltd
Priority to AU2014210372A priority Critical patent/AU2014210372B2/en
Publication of WO2014113846A1 publication Critical patent/WO2014113846A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)

Definitions

  • the present invention provides for compositions and methods for producing commercial barley plants thai are resistant to herbicides, in particular, the present invention provides for barley plants, plant tissue and plant seed that contain acetyl-CoA carboxylase (ACC) genes and proteins that confer resistance to inhibition by herbicides that normally inhibit the activity of the ACC protein.
  • ACC acetyl-CoA carboxylase
  • Barley (Hordeum vuigere) is a major cereal grain and is a member of the grass family. Barley is used in malt production, brewing and for the production oi cereal foods for human and animal consumption and is generally ranked in the top five cereal crops globally in terms of quantity produced and area under cultivation.
  • herbicides with greater potency, broad weed spectrum effectiveness and rapid soil degradation.
  • broad spectrum herbicides are those compounds that inhibit the activity of the acetyl-CoA carboxylase (ACC) enzyme in a plant. These are known as 'Group A ! or 'G o p T herbicides and include compounds within the following classes; aryloxyphenoxyproplonates (FOP); cyciohexanediortes (DIM); and phenyipyrazoiins.
  • FGP and DIM herbicides are generally the first line of choice for removing grass weeds in broadleaf (dicot) crops, most of them cannot be used selectively in cereals including barley. Therefore, grass weeds are sprayed with non-selective herbicides such as g!yphosate before seeding barley but grass weeds that emerge later in the crop cannot be controlled selectively at present.
  • the present invention overcomes some or all of the shortcomings of the prior art.
  • the present invention provides for barley plants and barley hybrids and methods for producing barley hybrids that are resistant to herbicides, in particular the present invention provides for bariey plants and hybrids, plant tissue and plant seed that contain acety!-CoA carboxylase (ACC) genes and proteins that confer resistance to Inhibition by herbicides that normally inhibit the activity of the AGC protein.
  • ACC acety!-CoA carboxylase
  • Cultivated commercial bariey is susceptible to many ACC inhibiting herbicides that target monocot or grassy weed species. However, as described herein a barley genotype was developed that exhibits tolerance to ACC inhibiting herbicides. Genetic analysis has Identified genetic differences within germplasm that results in a ACC herbicide resistant phenoiype.
  • the present invention provides for one or more barley plants whose germplasm comprises a mutation that renders the plani tolerant to ACC herbicides.
  • the invention relates to the offspring ⁇ e.g., Fl, F2, F3, etc.) of a cross of said plant wherein the germplasm of said offspring has the same mutation as the parent plant. Therefore, embodiments of the present invention provide for barley hybrids whose germplasm contains a mutation, such that the phenoiype of the plants is ACC herbicide resistant.
  • said offspring ⁇ e.g., Fl. F2, F3, etc.) are the result of a cross between elite bariey lines, at least one of which contains a germplasm comprising a mutation that renders the plant tolerant to ACC herbicides.
  • the Invention is a barley plant, wherein said barley plant confers resistance to inhibition by one or more acety!-CcA carboxylase inhibiting herbicides at levels that would normally inhibit the growth of a barley plant.
  • the barley plant is a commercial cultivar.
  • the acetyl-CoA carboxylase inhibiting herbicide is selected from the group consisting of; an aryloxyphenoxypropionate herbicide; and a cyciohexanedione herbicide.
  • the aryloxyphenoxypropionate herbicide is selected from the group consisting of; clodinafop-propargyi; diciofop-methyl; quiza!ofop-p-ethy!; fenoxyprop-p- ethyl; haloxyfop; fluazifop; and propaquizafop.
  • the cyciohexanedione herbicide is selected from the group consisting of; c!eihod!m; tralkoxydim; sethoxydim; tepra!oxydim; and butroxydim.
  • the barley plant comprises an acetyl-CoA carboxylase gene, wherein said gene corresponds to the amino acid substitution selected from the group consisting of: iie ( j 33 Leu; iie Vai; and !ie i 93S Leu and He zosaVai.
  • the !soieucine at position 1933 has beers substituted with a Leucine at the same position.
  • the barley plant comprises an acetyi-GoA carboxylase gene further comprising a polynucleotide that corresponds to a carboxyl transferase domain; and wherein the carboxyl transferase domain comprises anyone of the amino acids or polypeptides selected from the group consisting of;
  • the barley plant comprises an acety!-CcA carboxylase gene further comprising 3 polynucleotide that corresponds to a carboxyl transferase domain; and wherein the polynucleotide that corresponds to a carboxyl transferase domain comprises anyone of the nucleic acids and polynucleotides selected from the group consisting of;
  • the polynucleotide is introduced into the germpiasm of the barley plant by hybridisation.
  • the polynLideotsde Is introduced into the germpiasm of the bartey plant by introgression. Methods for these techniques are widely available in the art. See also Principles of Plant Breeding, 2nd ed By R. W. Allard. John Wiley & Sons, Mew York. 1999.
  • the polynucleotide is introduced into the germpiasm of the barley plant by recombinant DMA engineering techniques. Methods for these techniques are widely available in the art. See also in Vitro Plant Breeding S. Thirugrtanakumar, K. Manivannan, M, Prakash, R. Narasimman and Y. Anitha. Vasiine, Agrobios, 2009 in another preferred embodiment, the barley plant is not genetically modified.
  • the invention in a second aspect, is a seed of a barley plant as described herein.
  • the Invention is a germpiasm of a barley plant as described herein.
  • the invention is a method of producing a barley hybrid with increased resistance to inhibition by one or more aceiyi-CoA carboxylase inhibiting herbicides at levels that would normally inhibit the growth of a arley plant, said method comprising; selecting a first barley plant;
  • hybrid offspring selecting a hybrid from said hybrid offspring with increased resistance to inhibition by one or more acetyl-CoA carboxylase inhibiting herbicides thereby to produce the hybrid:
  • the second barley plant is a barley plant as described herein.
  • the invention is a method of producing a barley hybrid with increased resistance to inhibition by one or more acetyl-GoA carboxylase inhibiting herbicides at levels that would normally inhibit the growth of a barley plant, said method comprising; selecting a first barley piant;
  • CoA carboxylase Inhibiting herbicides thereby to produce the barley hybrid and wherein the foreign polynucleotide is selected from polynucleotides that correspond to a carboxy! transferase domain of a second barley plant:
  • the second barley plant is a barley plant as described herein; and wherein the foreign polynucleotide is selected from the group consisting of;
  • the first barley plant is selected from the group consisting of AC Metcalfe, Bass, Baudin, Bu!oke, CDC Cope!and, CDC Reserve, Commander, Fairview, Fathom, Flagship, Fleet, Gairdner, Henley, Hindmarsh, Keel, Navigator, Oxford, Propino, Scope, Craig, Skipper, SouthemStar, Quench, Vlamingh, V Admiral, Riverside, WI4593 and Wimmera.
  • the second barley plant is selected from the group consisting of; BOZO; and HT017.
  • the Invention is a barley hybrid produced by the methods herein described.
  • the hybrid comprises an acetyi-CoA carboxylase gene, wherein said gene corresponds to the amino acid substitution selected from the group consisting of: ile ⁇ ssLeu; !ieaei & Va!; and ile !83S Leu and liejooaVai.
  • the hybrid comprises a acetyl-GoA carboxylase gene, further comprising a polynucleotide that corresponds to a carboxyi transferase domain; and
  • carboxyl transferase domain comprises anyone of the amino acids or polypeptides selected from the group consisting of;
  • the hybrid comprises an acetyi-CoA carboxylase gene further comprising a polynucleotide that corresponds to a carboxyi transferase domain;
  • polynucleotide that corresponds to a carboxyl transferase domain comprises nucleic acids selected from the group consisting of;
  • the barley hybrid is a commercial cu!iivar. That is, it is adapted for us in the commercial production of barley.
  • the Invention is a seed of a bariey hybrid as herein described.
  • the invention is germp!asm of s barley hybrid as herein described.
  • the invention is a method of producing bariey, the method comprising;
  • barley plant has increased resistance to inhibition by one or more acety!-CoA carboxylase inhibiting herbicides at levels that would normally inhibit the growth of a bariey plant.
  • the invention is a method of producing barley, the method comprising;
  • hybrid has increased resistance to Inhibition by one or more acetyi-CoA carboxylase inhibiting herbicides at Ievels that would normally inhibit the growth of a barley hybrid.
  • the methods of the ninth and tenth aspects of the invention improve weed control compared to weed control normally observed for producing bariey.
  • the methods of the ninth and tenth aspeds of the invention decrease contamination with weed species compared to contamination normally observed for producing barley.
  • the Invention provides a method of controlling weeds in the vicinity of a bariey plant as described herein, comprising:
  • the invention provides a method of controlling weeds in the vicinity of a bariey hybrid as described herein, comprising:
  • the acetyl- CoA carboxylase inhibiting herbicide is selected from the group consisting of; an aryloxyphenoxypropionate herbicide and a cyciohexanedione herbicide.
  • the aryioxyphertoxypropionate herbicide is selected from the group consisting of; e!odinafop- propargy!; dic!ofop-methyl; fenoxyprop-p-ethy!; quizalofop-p-sthyl; haloxyfop; fluazifop; and propaquizafop.
  • the cyciohexanedione herbicide is selected from the group consisting of; ctethodim; sethoxydim; tepraloxydim; tepraloxydim; tralkoxydim; and butroxydim.
  • the phenylpyrazo!in herbicide is pinoxaden
  • the one or more acetyi-CoA carboxylase inhibiting herbicides is applied at a rate selected from the group consisting of: between 5m!/ha and l OOOml/ha; between l Omi/ha and 50Qml/ha; between 50m! 'ha and 400ml/ha; between 10Gmi/ha and 400m!/ha; between 200rni/ha and 400mi/ha.
  • the invention is a barley plant or barley hybrid as described herein, wherein said barley plant or said bariey hybrid confers resistance to inhibition by one or more acetyl-GoA carboxylase inhibiting herbicides at levels that would normally inhibit the growth of a bariey plant, wherein said levels are selected from the group consisting of the following herbicide application rates: between 5mi/h3 and 1000mi/ha; between 10ml/ha
  • the carboxyi transferase domain of the barley plant or hybrid as described herein does nol have 100% sequence homology to the following amino acid sequence : ,s 3 cCys Gki A.sn Leu His G!y Ser es .
  • the barley plant or barley hybrid as described herein is sensitive to acetolactate synthase enzyme inhibitor herbicides.
  • the barley plant is sensitive to acetolactate synthase enzyme inhibitors herbicides selected from the group consisting of: Imidazolines and sulfonylureas.
  • FIG. 2 This figure presents theticians! data of the effects of four ACCase- inhibiting FOP herbicides on the grain yield of BOZO and cuitivar Fleet as discussed in Example 2.
  • BOZO is shown to the left of each photo and cuitivar Fleet is shown in the right of each photo.
  • Herbicide was applied perpendicular to sowing direction (left to right).
  • B - 19 th of August 2011 23 DAA
  • O - 11 !!l of November 2011 108 DAA
  • Figure 3 This figure presents the graphical representation of the effects of three ACGase- inhibiting DIM herbicides on the grain yield of barley mutant BOZO and cuitivar Fleet as discussed in Example 2. Treatments with the same letter above the bar are not statistically significant based on the least significant difference at P ⁇ Q.05.
  • Figure 4 This figure presents HT017 three weeks after treatment with Fusi!ade in comparison to sensitive genotypes.
  • the invention described herein may include one or more ranges of values (e.g. size, concentration etc).
  • a range of values will be understood to include ali values within the range, including the values defining the range, and values adjacent to the range that lead to the same or substantially the same outcome as the values immediately adjacent to that value which defines the boundary to the range.
  • the term "barley plant” refers to the species Hordeum vulgars in its total genetic variation and its many varieties aiid to related species within the Hordeum genus. For example, the term includes the following varieties: AC Metcalfe, Bass, Baudlrt, Buioke, CDC Copeland. CDC Reserve, Commander, Fairview. Fathom, Flagship, Fleet, Gairdner, Henley, 5 Hindmars , Keel, Navigator, Oxford, Propirto, Scope, Sebastian, Skipper, SouihernStar, Quench, Vlamingh, VT Admiral, Riverside, WI4593 and Wimmera.
  • the term includes tolerance to herbicides and reduced sensitivity.
  • the herbicide does not kill the herbicide resistant barley.
  • the herbicide only kiiis a small percentage of herbicide resistant barley plants in a crop selected from a group of percentage ranges consisting of: less than 1 % of the barley crop; less than 2%; less than 5%; less than 10%; less than 20%; less than
  • the herbicide does not reduce the growth rate of the herbicide resistant barley, in one embodiment, the herbicide reduces the growth rate of the herbicide resistant barley by a percentage range selected from the group consisting of. less than a 1% reduction in growth rate; less than 2%. !ess than 5%; less than 10%; less than 20%; less than 30%; less than 40%; and less than 50%.
  • the herbicide does not reduce the yield rate of the herbicide resistant barley, in one embodiment, the herbicide only reduces the yield rate of the herbicide resistant barley crop by a percentage range selected from the group consisting of: less than 1 % of the herbicide resistant barley crop: less than 2%; less than 5%; less than 0%; less than 20%; less than 30%: less than 40%; and less than 50%.
  • acetyS-GoA carboxylase inhibiting herbicides are those herbicides that inhibit acetyi-GoA carboxylase (ACC) enzyme in a plant. These are known as 'Group A' or 'Group 1 * herbicides and include compounds within the following classes; ary!oxyphenoxypropionates (FOP); cyclohexanediones (DIM); and phenylpyrazoiins.
  • carboxyl transferase domain refers to the domain on a polypeptide or protein 30 which carries out the reaction of transcarboxyiation from biotin to an acceptor molecule.
  • the invention further provides a seed of the non-naiurally occurring barley plant which has increased resistance to herbicides.
  • the increased herbicide resistance developed by the present inventors results from changes to the physiolog and/or biochemistry of the p!ant and thus interferes with the norma! action of the herbicide. This may involve altered absorption and/or translocation of ihe herbicide or altered ability of metabolic enzymes to bind the herbicide.
  • the invention extends to the altered elements in the plant that confer herbicide resistance including altered gene(s) and/or proteln(s) including enzymes, which, genes and proteins are altered compared to their counterparts in herbicide sensitive barley plants.
  • said altered genes and/or proteins are in isolated or substantially purified form.
  • the inventors developed a barley variety, named 'BOZO', whose phenotype is a reduced sensitivity to ACC herbicides. Genetic analysis has identified genetic differences within the germp!asm of BOZO (compared barley plants that are ordinarily sensitive to ACC herbicides) that results in an ACC herbicide resistant phenotype. The genotype of BOZO was compared to three different barley varieties.
  • Genomic DMA was extracted from a single cotyledon of 6-day old seedlings and used as the template for polymerase chain reactions (PCR).
  • Three sets of PGR primers were designed based on the published sequence of aceiyi-CoA carboxylase rnRNA from black grass ASopecurus myosuroides (GenBank: AJ310787) to amplify approximately 7.5 kb of carboxyi-transferase (CT) domain encompassing the regions known to be involved in sensitivity to ACCase herbicides (De ' lye C and Michel S 2005 "Universal" primers for
  • SNP single nucleotide polymorphism
  • the aim of the trial was to investigate tolerance of BOZO to four AGCase-inhibiting FOP herbicides, three AGCase-inhibiting DIM herbicides and one ALS-inhibiting imidazolinone herbicide under field conditions.
  • herbicide treatments were applied on the 26* of July at 49 days after sowing when barley was at mid ti!ering (approximately Z22-Z25). Herbicide treatments were applied with a spray boom delivering 100L'ha spray volume with surfactants used according to herbicide labels. Various herbicide treatments used in this study are shown in Table 1. Measurements recorded induded crop establishment, normalised difference vegetation index (NDV!), crop canopy height at maturity, grain yield, grain size and visual observations of crop damage from the treatments.
  • NDV! normalised difference vegetation index
  • BOZO mutant barley showed reduction in yield at 1 ⁇ 2x and 1x rate but there was 28% reduction at the 2x rale of ha!oxyfop.
  • the differences in herbicide tolerance between Fleet and BOZO were also apparent for fiuazifop and propaquizafop ( Figure 1).
  • BOZO Pot Experiment 1 (201 Q): Quizalofop at 0, 0.25, 0.5, 1.0, and 2.0 x recommended label rate (30g/ha) across SOZO mutant barley, Flagship barley cultivar and barley grass ⁇ Hordeum giaucum). Visual observations were taken.
  • BOZO Po .. tperjment 2 (201 . 0): The objective was to confirm tolerance to quizalofop in BOZO mutant barley and investigate tolerance to other herbicides. Herbicides included
  • Herbicides included quizalofop, haloxyfop (ACCase-inhibiting FOP), c!ethodim, tepraloxydim, butrox dim (ACCase- inhiblting DIM), iodosulfuron, mesosu!furon ⁇ ALS-inhibiting sulfonylureas), imazamox /imazapyr (ALS-inhibiting imidazoiinones), and glyphosate ⁇ membrane disrupters glycines). Rates applied were 0, 0.25, 0.5, 1.0 £ 2.0 x recommended label rates and both BOZO mutant barley and FLEET barley CLiltivar were screened. Visual observations and plant biomass measurements were made.
  • BOZO Pot Expsrimerst 4 (2011 ): The objective was to Investigate herbicide tolerance of BOZO mutant barley to quizalofop, haloxyfop, fluazifop, propaquizafop (ACCass- inhibiting FOP), and sethoxydim (ACCase-inhibiting DIM) herbicides. Rates applied were 0, 0.25, 0.5, 10 & 2.0 x field rates and both BOZO mutant barley and Fleet barley cultlvar were screened. Visual observations and plant biomass measurements were made.
  • BOZQ field experiment AC-D8 (2811 ): The objective was to investigate herbicide tolerances of BOZO mutant barley and barley grass ⁇ Hordeum glaucum) control. This field site at Roseworthy SA had low fertility and treatments included quizalofop, haloxyfop, fluazifop, (ACCase-inhibiting FOP), clethodim, tepraioxydim, buiroxydim (ACCase- inhiblting DIM) herbicides at 0, 0.5, 1.0, and 2.0 x recommended label rates. Visual assessment of crop damage, barley grass control, NDV!, crap canopy height at maturity, grain yield, and grain size was measured.
  • BOZO field K sgrimaot AC-S2 (2811 ): reported above. The objective was to investigate herbicide tolerance of BOZO mutant barley in a low rainfall environment and brome grass (Bromus rigidus) control. This field site had low fertility and herbicide treatments included
  • BOZQ._fiejd exp rim nt BUCKLEBOO ,12011 The objective was to investigate herbicide tolerance of BOZO mutant barley in a low rainfaii environment as well as barley grass (Hordeu glaucu n) control. Herbicide treatments were applied at 1 & 2 x recommended label rates and included quizalofop (ACCase-inhibiting FOP), c!eihoalm (ACCase-inhibiting DiM), and imazamox /imazapyr (ALS-inhibiting imidazo!inones). Crop damage was assessed based on visual symptoms and panicle density was used to determine barley grass control.
  • the inventors developed a barley variety, named 'HT017', whose phenotype is a reduced sensitivity to ACC herbicides.
  • Seed of the variety Flagship was mutagenised with 30mM ethyl methanesuifanaie (EMS) and the 0 and generations were grown and multiplied as unse!ected bulk populations.
  • the M 2 generation was sown at the Chariick Experimental Farm (Stratschyn, South Australia) on 20 t!> Ma 2009 at a density of 140 plants rrf ⁇
  • the area sown was 2,5 hectares equating to approximately 40 million individual plants.
  • the herbicide Fusilade (fluazifop) was applied a; a rate of SOOg / ha at the early tillering stage of plant development.
  • At stem elongation surviving barley plants were transplanted to pots, grown through to maturity and grain was harvested from individual plants. Seed from the surviving plants was sown in pots in 6 ay 2010 and treated with SOQg / ha Fusilade at the 3 leaf stage of development.
  • Assessment of survival was conducted three weeks after spraying and -99% of plants were found to be sensitive to the herbicide but the line HT017 was confirmed as tolerant to fluazifop.
  • Figure 4 presents HT017 three weeks after treatment with fluazifop in comparison to sensitive genotypes.
  • SNP single rtuc!eotide polymorphism
  • HT017 to Fusilade was verified in a pot experiment.
  • HT017 and the intolerant control barley HT066 were sown on 23 rci June 2010 with three replicates per treatment.
  • Two treatments comprising SOOg/ha Fusilidate and 750g/ha Fusilade were applied on 23 :d July 2010 and one treatment remained unsprayed.
  • the trial was assessed on 18 ai August 2010 and all HT066 plants were destroyed at both application rates. Ail HT017 plants survived at both application rates.
  • the mutant barley HT017 was tested for tolerance against a range of Group A herbicides. Three replicate pots were sown for each herbicide treatment of the tolerant line HT017 and also the control barley HT086. Seed was sown 17 ! August 2010 and plants were

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des plants d'orge résistant aux herbicides et des compositions ainsi que des procédés pour produire des hybrides de l'orge résistant aux herbicides, en particulier des plants d'orge renfermant des gènes de l'acétyl-CoA carboxylase (ACCase) qui sont résistants aux herbicides inhibiteurs de l'ACCase, par exemple les variétés d'orge BOZO et HT107.
PCT/AU2014/000060 2013-01-25 2014-01-24 Orge tolérant les herbicides WO2014113846A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2014210372A AU2014210372B2 (en) 2013-01-25 2014-01-24 Herbicide tolerant barley

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2013900274A AU2013900274A0 (en) 2013-01-25 Herbicide Tolerant Barley
AU2013900274 2013-01-25

Publications (1)

Publication Number Publication Date
WO2014113846A1 true WO2014113846A1 (fr) 2014-07-31

Family

ID=51226761

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU2014/000060 WO2014113846A1 (fr) 2013-01-25 2014-01-24 Orge tolérant les herbicides

Country Status (3)

Country Link
AR (1) AR094594A1 (fr)
AU (1) AU2014210372B2 (fr)
WO (1) WO2014113846A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012106321A1 (fr) * 2011-02-01 2012-08-09 Colorado Wheat Research Foundation, Inc. Plantes résistantes aux herbicides présentant une activité de l'acétyl-coenzyme a carboxylase
US20120284853A1 (en) * 2009-09-01 2012-11-08 Basf Agrochemical Products, B.V. Herbicide-tolerant plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120284853A1 (en) * 2009-09-01 2012-11-08 Basf Agrochemical Products, B.V. Herbicide-tolerant plants
WO2012106321A1 (fr) * 2011-02-01 2012-08-09 Colorado Wheat Research Foundation, Inc. Plantes résistantes aux herbicides présentant une activité de l'acétyl-coenzyme a carboxylase

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MATTHEWS, N. ET AL.: "Mechanisms of resistance to acetyl-coenzyme A carboxylase- inhibiting herbicides in a Hordeum leporinum population'.", PEST MANAGEMENT SCIENCE, vol. 56, 2000, pages 441 - 447 *

Also Published As

Publication number Publication date
AU2014210372B2 (en) 2018-03-01
AU2014210372A1 (en) 2015-09-03
AR094594A1 (es) 2015-08-12

Similar Documents

Publication Publication Date Title
US20210403934A1 (en) Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof
US10100328B2 (en) Corn plant event MON87460 and compositions and methods for detection thereof
EP2503871B1 (fr) Événement 416 de la transformation aad-12, lignées de soja transgéniques associées, et leur identification spécifique à l'événement
KR101899619B1 (ko) Aad-1 식물에 관련된 잡초 방제 방법 및 식재-전 및/또는 발아-전 제초제 적용
CN102808017A (zh) 具有多个除草剂抗性ahasl1等位基因的除草剂抗性向日葵植物及使用方法
CN102947443A (zh) 耐受除草剂的植物
US20210352863A1 (en) Method for controlling weed beets and other weeds
AU2017217445A1 (en) Herbicide safener combinations for acetyl co-enzyme a carboxylase herbicide resistant plants
CN107299149B (zh) 利用分子标记辅助培育抗除草剂咪草烟粳稻品种的方法
KR102576009B1 (ko) 벼에서 제초제 저항성/내성과 연관된 돌연변이의 조합을 위한 방법 및 조성물
WO2014113846A1 (fr) Orge tolérant les herbicides
Lewis Novel Herbicide Resistance Trait Investigation and QTL Mapping in Wheat
US20220039390A1 (en) Use of glyphosate herbicide for controlling unwanted vegetation in beta vulgaris growing areas
Simelane Molecular characterization of herbicide resistance in Palmer amaranth (Amaranthus palmeri S. Wats) populations in South Africa
WO2023126317A1 (fr) Plante tolérante aux herbicides inhibiteurs hppd
Initiative Target-site EPSPS Pro-106 mutations: sufficient to endow glyphosate resistance in polyploid Echinochloa colona?
White Characterization of acetolactate synthase resistance in common sunflower (Helianthus annuus)
NZ615200B2 (en) Methods of weed control involving aad-1 plants, and re-plant and/or pre-emergence herbicide applications

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14742747

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2014210372

Country of ref document: AU

Date of ref document: 20140124

Kind code of ref document: A

122 Ep: pct application non-entry in european phase

Ref document number: 14742747

Country of ref document: EP

Kind code of ref document: A1