WO2014087241A3 - Methods of detecting biofilms from the palmitoylation of lipopolysaccharides - Google Patents

Methods of detecting biofilms from the palmitoylation of lipopolysaccharides Download PDF

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Publication number
WO2014087241A3
WO2014087241A3 PCT/IB2013/003113 IB2013003113W WO2014087241A3 WO 2014087241 A3 WO2014087241 A3 WO 2014087241A3 IB 2013003113 W IB2013003113 W IB 2013003113W WO 2014087241 A3 WO2014087241 A3 WO 2014087241A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
lipopolysaccharides
detecting
palmitoylation
present technology
Prior art date
Application number
PCT/IB2013/003113
Other languages
French (fr)
Other versions
WO2014087241A2 (en
Inventor
Jean-Marc Ghigo
Sabina CHALABAEV
Christophe Beloin
Martine Caroff
Alexey NOVIKOV
Original Assignee
Institut Pasteur
Centre National De La Recherche Scientifique (Cnrs)
Universite Paris Sud 11
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur, Centre National De La Recherche Scientifique (Cnrs), Universite Paris Sud 11 filed Critical Institut Pasteur
Publication of WO2014087241A2 publication Critical patent/WO2014087241A2/en
Publication of WO2014087241A3 publication Critical patent/WO2014087241A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50—Lipopolysaccharides; LPS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/10—Post-translational modifications [PTMs] in chemical analysis of biological material acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

The present technology provides an apparatus and methods for detecting Gram negative bacteria biofilm, where the methods include extracting bacterial lipopolysaccharides (LPS) from the biological sample and detecting the level of palmitoylated lipid A in isolated or extracted lipopolysaccharides. The present technology further relates to methods for optimizing therapeutic efficacy for treatment of a Gram negative bacterial infection.
PCT/IB2013/003113 2012-12-05 2013-12-04 Methods of detecting biofilms from the palmitoylation of lipopolysaccharides WO2014087241A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261733861P 2012-12-05 2012-12-05
US61/733,861 2012-12-05

Publications (2)

Publication Number Publication Date
WO2014087241A2 WO2014087241A2 (en) 2014-06-12
WO2014087241A3 true WO2014087241A3 (en) 2014-11-20

Family

ID=50280434

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2013/003113 WO2014087241A2 (en) 2012-12-05 2013-12-04 Methods of detecting biofilms from the palmitoylation of lipopolysaccharides

Country Status (1)

Country Link
WO (1) WO2014087241A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107022508A (en) * 2017-04-21 2017-08-08 华东理工大学 A kind of Escherichia coli O 157：The cultural method of H7 mycoderms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011091322A2 (en) * 2010-01-22 2011-07-28 Hyprotek, Inc. Antimicrobial agents and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011091322A2 (en) * 2010-01-22 2011-07-28 Hyprotek, Inc. Antimicrobial agents and methods of use

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ANDREW PRESTON ET AL: "Bordetella bronchiseptica PagP is a Bvg-regulated lipid A palmitoyl transferase that is required for persistent colonization of the mouse respiratory tract", MOLECULAR MICROBIOLOGY, vol. 48, no. 3, 1 May 2003 (2003-05-01), pages 725 - 736, XP055120151, ISSN: 0950-382X, DOI: 10.1046/j.1365-2958.2003.03484.x *
CIORNEI, CD, NOVIKOV A, BELOIN C, FITTING C, CAROFF M, GHIGO J-M, CAVAILLON, J-M, ADIB-CONGUY, M: "Biofilm-forming Pseudomonas aeruginosa bacteria undergo lipopolysaccharide structural modifications and induce enhanced inflammatory cytokine response in human monocytes", INNATE IMMUNITY, vol. 16, no. 5, 1 October 2010 (2010-10-01), pages 288 - 301, XP008169548 *
KAWASAKI K, ERNST RK, MILLER SI: "Deacylation and palmitoylation of lipid A by Salmonellae outer membrane enzymes modulate host signaling through Toll-like receptor 4", JOURNAL OF ENDOTOXIN RESEARCH, vol. 10, 1 October 2004 (2004-10-01), pages 439 - 444, XP008169564 *
LUCA FERNNDEZ ET AL: "Creeping baselines and adaptive resistance to antibiotics", DRUG RESISTANCE UPDATES, CHURCHILL LIVINGSTONE, EDINBURGH, GB, vol. 14, no. 1, 5 January 2011 (2011-01-05), pages 1 - 21, XP028147462, ISSN: 1368-7646, [retrieved on 20110111], DOI: 10.1016/J.DRUP.2011.01.001 *
N. E. HEAD ET AL: "Cross-Sectional Analysis of Clinical and Environmental Isolates of Pseudomonas aeruginosa: Biofilm Formation, Virulence, and Genome Diversity", INFECTION AND IMMUNITY, vol. 72, no. 1, 19 December 2003 (2003-12-19), pages 133 - 144, XP055120139, ISSN: 0019-9567, DOI: 10.1128/IAI.72.1.133-144.2004 *
R. K. ERNST: "Specific Lipopolysaccharide Found in Cystic Fibrosis Airway Pseudomonas aeruginosa", SCIENCE, vol. 286, no. 5444, 19 November 1999 (1999-11-19), pages 1561 - 1565, XP055120145, ISSN: 0036-8075, DOI: 10.1126/science.286.5444.1561 *
RAETZ C R H ET AL: "LIPOPOLYSACCHARIDE ENDOTOXINS", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 71, 1 January 2002 (2002-01-01), pages 635 - 700, XP001155986, ISSN: 0066-4154, DOI: 10.1146/ANNUREV.BIOCHEM.71.110601.135414 *
ROBERT K. ERNST ET AL: "Unique Lipid A Modifications in Pseudomonas aeruginosa Isolated from the Airways of Patients with Cystic Fibrosis", THE JOURNAL OF INFECTIOUS DISEASES, vol. 196, no. 7, 1 October 2007 (2007-10-01), pages 1088 - 1092, XP055120137, ISSN: 0022-1899, DOI: 10.1086/521367 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107022508A (en) * 2017-04-21 2017-08-08 华东理工大学 A kind of Escherichia coli O 157：The cultural method of H7 mycoderms

Also Published As

Publication number Publication date
WO2014087241A2 (en) 2014-06-12

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