WO2014023947A1 - Méthode permettant d'identifier une interaction de liaison spécifique entre deux polypeptides ou plus - Google Patents

Méthode permettant d'identifier une interaction de liaison spécifique entre deux polypeptides ou plus Download PDF

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Publication number
WO2014023947A1
WO2014023947A1 PCT/GB2013/052092 GB2013052092W WO2014023947A1 WO 2014023947 A1 WO2014023947 A1 WO 2014023947A1 GB 2013052092 W GB2013052092 W GB 2013052092W WO 2014023947 A1 WO2014023947 A1 WO 2014023947A1
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide
tag
polysome
polypeptides
sequence
Prior art date
Application number
PCT/GB2013/052092
Other languages
English (en)
Inventor
Angray S. Kang
Original Assignee
Queen Mary And Westfield College University Of London
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Queen Mary And Westfield College University Of London filed Critical Queen Mary And Westfield College University Of London
Publication of WO2014023947A1 publication Critical patent/WO2014023947A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/18—Dental and oral disorders

Abstract

Cette invention concerne une méthode permettant d'identifier une interaction de liaison spécifique entre un premier polypeptide et un second polypeptide qui comprend les étapes suivantes : (i) combinaison a) d'un premier polysome qui comprend le premier polypeptide et une première étiquette avec b) un second polysome qui comprend le second polypeptide et une seconde étiquette ; et (ii) recherche pour déterminer si un complexe qui comprend à la fois la première étiquette et la seconde étiquette se forme ou non entre les premier et second polysomes, la formation d'un complexe qui comprend à la fois la première et la seconde étiquette indiquant que le premier polypeptide et le second polypeptide interagissent de manière spécifique.
PCT/GB2013/052092 2012-08-07 2013-08-06 Méthode permettant d'identifier une interaction de liaison spécifique entre deux polypeptides ou plus WO2014023947A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1214029.9 2012-08-07
GB201214029A GB201214029D0 (en) 2012-08-07 2012-08-07 method

Publications (1)

Publication Number Publication Date
WO2014023947A1 true WO2014023947A1 (fr) 2014-02-13

Family

ID=46934979

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2013/052092 WO2014023947A1 (fr) 2012-08-07 2013-08-06 Méthode permettant d'identifier une interaction de liaison spécifique entre deux polypeptides ou plus

Country Status (2)

Country Link
GB (1) GB201214029D0 (fr)
WO (1) WO2014023947A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10086056B2 (en) 2015-01-15 2018-10-02 University Of Copenhagen Virus-like particle with efficient epitope display
US11129882B2 (en) 2015-10-30 2021-09-28 University Of Copenhagen Virus like particle with efficient epitope display

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991005058A1 (fr) * 1989-10-05 1991-04-18 Glenn Kawasaki Synthese et isolation sans cellule de nouveaux genes et de nouveaux polypeptides
WO1999011777A1 (fr) * 1997-09-03 1999-03-11 Biovation Limited Procedes d'analyse de proteines

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991005058A1 (fr) * 1989-10-05 1991-04-18 Glenn Kawasaki Synthese et isolation sans cellule de nouveaux genes et de nouveaux polypeptides
WO1999011777A1 (fr) * 1997-09-03 1999-03-11 Biovation Limited Procedes d'analyse de proteines

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ROBERTS R W: "Totally in vitro protein selection using mRNA-protein fusions and ribosome display", CURRENT OPINION IN CHEMICAL BIOLOGY, CURRENT BIOLOGY LTD, LONDON, GB, vol. 3, 1 January 1999 (1999-01-01), pages 268 - 273, XP002180767, ISSN: 1367-5931, DOI: 10.1016/S1367-5931(99)80042-8 *
ROBIN HURST ET AL: "Protein-protein interaction studies on protein arrays: Effect of detection strategies on signal-to-background ratios", ANALYTICAL BIOCHEMISTRY, vol. 392, no. 1, 1 September 2009 (2009-09-01), pages 45 - 53, XP055085311, ISSN: 0003-2697, DOI: 10.1016/j.ab.2009.05.028 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10086056B2 (en) 2015-01-15 2018-10-02 University Of Copenhagen Virus-like particle with efficient epitope display
US10526376B2 (en) 2015-01-15 2020-01-07 University Of Copenhagen Virus-like particle with efficient epitope display
US11497800B2 (en) 2015-01-15 2022-11-15 University Of Copenhagen Virus-like particle with efficient epitope display
US11129882B2 (en) 2015-10-30 2021-09-28 University Of Copenhagen Virus like particle with efficient epitope display

Also Published As

Publication number Publication date
GB201214029D0 (en) 2012-09-19

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