WO2014022700A3 - Functionally integrated device for multiplex genetic identification and method for separation and detection of dna fragments - Google Patents

Functionally integrated device for multiplex genetic identification and method for separation and detection of dna fragments Download PDF

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Publication number
WO2014022700A3
WO2014022700A3 PCT/US2013/053284 US2013053284W WO2014022700A3 WO 2014022700 A3 WO2014022700 A3 WO 2014022700A3 US 2013053284 W US2013053284 W US 2013053284W WO 2014022700 A3 WO2014022700 A3 WO 2014022700A3
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WO
WIPO (PCT)
Prior art keywords
dna fragments
separation
nucleic acid
probes
detection
Prior art date
Application number
PCT/US2013/053284
Other languages
French (fr)
Other versions
WO2014022700A2 (en
Inventor
Ranjit A. PRAKASH
Original Assignee
Prakash Ranjit A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/955,690 external-priority patent/US20140200167A1/en
Priority claimed from US13/955,641 external-priority patent/US20140080725A1/en
Application filed by Prakash Ranjit A filed Critical Prakash Ranjit A
Publication of WO2014022700A2 publication Critical patent/WO2014022700A2/en
Publication of WO2014022700A3 publication Critical patent/WO2014022700A3/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A bsochip for multiplex genetic identification is disclosed. An bsochip for separating and detecting a plurality of DNA fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material. The bioehip also includes a plurality of separation and detection chambers that physically separate different DNA fragments that have been marked with labels that emit similar colors. A method of detecting nucleic acid fragments is also provided. Each chamber has at least one set of probes disposed therein, each set of probes being capable of binding to a different target nucleic acid sequence relative to the other sets of probes. The method includes providing a sample comprising a plurality of sets of nucleic acid fragments, placing at least a portion of the sample into each of the plurality of chambers.
PCT/US2013/053284 2012-08-01 2013-08-01 Functionally integrated device for multiplex genetic identification and method for separation and detection of dna fragments WO2014022700A2 (en)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US201261678363P 2012-08-01 2012-08-01
US201261678364P 2012-08-01 2012-08-01
US61/678,364 2012-08-01
US61/678,363 2012-08-01
US13/955,690 US20140200167A1 (en) 2012-08-01 2013-07-31 Functionally integrated device for multiplex genetic identification
US13/955,641 2013-07-31
US13/955,690 2013-07-31
US13/955,659 2013-07-31
US13/955,641 US20140080725A1 (en) 2012-08-01 2013-07-31 Method for separation and detection of dna fragments
US13/955,659 US20140080726A1 (en) 2012-08-01 2013-07-31 Enhanced method for probe based detection of nucleic acids

Publications (2)

Publication Number Publication Date
WO2014022700A2 WO2014022700A2 (en) 2014-02-06
WO2014022700A3 true WO2014022700A3 (en) 2014-04-17

Family

ID=50028666

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/053284 WO2014022700A2 (en) 2012-08-01 2013-08-01 Functionally integrated device for multiplex genetic identification and method for separation and detection of dna fragments

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016004539A1 (en) * 2014-07-11 2016-01-14 Advanced Theranostics Inc. Point of care polymerase chain reaction device for disease detection
US10690627B2 (en) * 2014-10-22 2020-06-23 IntegenX, Inc. Systems and methods for sample preparation, processing and analysis
EP3344391B1 (en) * 2015-09-04 2023-03-15 Life Technologies Corporation Devices and methods for mesofluidic and/or microfluidic processes
EP3400284A4 (en) 2016-01-08 2019-10-23 Advanced Theranostics Inc. Fully integrated, stand-alone, point-of-care device to detect target nucleic acids
EP3562929B1 (en) * 2016-12-29 2024-05-01 Ador Diagnostics S.r.l. An electrophoretic chip for electrophoretic applications
CN111366725A (en) * 2018-12-26 2020-07-03 台达电子工业股份有限公司 Detection method and device for enhancing detection signal and test piece
CN118146913A (en) * 2024-05-13 2024-06-07 苏州天隆生物科技有限公司 Kit, kit manufacturing method and amplification detection method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002043864A2 (en) * 2000-11-03 2002-06-06 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
US20050074784A1 (en) * 2003-10-07 2005-04-07 Tuan Vo-Dinh Integrated biochip with continuous sampling and processing (CSP) system
US20060275789A1 (en) * 2000-10-24 2006-12-07 Willis Thomas D Direct multiplex characterization of genomic DNA
US20100144558A1 (en) * 2006-08-25 2010-06-10 Frederic Zenhausern Systems and methods for biodosimetry with biochip using gene expression signatures
US20110180425A1 (en) * 1999-04-21 2011-07-28 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110180425A1 (en) * 1999-04-21 2011-07-28 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
US20060275789A1 (en) * 2000-10-24 2006-12-07 Willis Thomas D Direct multiplex characterization of genomic DNA
WO2002043864A2 (en) * 2000-11-03 2002-06-06 Clinical Micro Sensors, Inc. Devices and methods for biochip multiplexing
US20050074784A1 (en) * 2003-10-07 2005-04-07 Tuan Vo-Dinh Integrated biochip with continuous sampling and processing (CSP) system
US20100144558A1 (en) * 2006-08-25 2010-06-10 Frederic Zenhausern Systems and methods for biodosimetry with biochip using gene expression signatures

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Publication number Publication date
WO2014022700A2 (en) 2014-02-06

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