WO2013190143A1

WO2013190143A1 - Use of aleuroglyphus ovatus for breeding predatory mites

Info

Publication number: WO2013190143A1
Application number: PCT/EP2013/063136
Authority: WO
Inventors: Sabine GUICHOU; Pascal Maignet; Maxime FERRERO; Serge KREITER
Original assignee: Invivo Agrosolutions; Centre International D'etudes Superieures En Sciences Agronomiques
Priority date: 2012-06-22
Filing date: 2013-06-24
Publication date: 2013-12-27
Also published as: FR2992149B1; FR2992149A1

Abstract

The present invention relates to the use of a population of Aleuroglyphus ovatus for breeding a population of predatory mites, in particular of the species Neoseiulus cucumeris. A first subject of the invention is the use of a population of Aleuroglyphus ovatus as a source of nutrients for breeding a population of predatory mites. A second subject of the invention is a biological control composition that includes: (i) a source of nutrients that includes or consists of a population of Aleuroglyphus ovatus; (ii) at least one population of predatory mites, preferably selected from the list consisting of: Neoseiulus cucumeris, Amblyseius largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius finlandicus, Euseius ovalis, Euseius scutalis, Euseius hibisci, Euseius stipulatus, Galendromus occidentalis, Iphiseius degenerans, Macrocheles robustulus, Hypoaspis miles, Pergamasus quisquiliarum, Phytoseius finitimus, Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri and Typhlodromalus peregrinus; (iii) optionally a support and/or distribution substrate.

Description

USE OF ALEUROGLYPHUS OVATUS FOR BREEDING

PREDATORY MITES.

The present invention relates to the use of a population & Aleuroglyphus ovatus, for breeding a population of predatory mites, in particular of the species Neoseiulus cucumeris.

The development of phytosanitary products has allowed a considerable expansion of agriculture by allowing treatment against a wide variety of pests but also diseases, with a limited number of molecules. The effects of plant protection products depend essentially on their category: insecticides, acaricides, fungicides, herbicides, nematicides, molluscicides, rodenticides, taupicides.

The use of these products, however, contributes to the imbalance of the biotope, in particular by eliminating certain auxiliary species and / or by causing resistance phenomena, two factors contributing to the development of pests and pathogens harmful to crops.

The use of biological control agents is a method of controlling pests, pathogens or weeds by means of natural organisms that are antagonistic to them, such as phytophages (in the case of a weed plant ), parasitoids (arthropods ...), predators (nematodes, arthropods, vertebrates, molluscs, bats ...) or pathogens (viruses, bacteria, fungi ...). The use of these biological control agents also allows a reduction in the use of pesticides.

This approach requires reintroduction of the biological control agent in the form of live predators and in sufficient numbers to achieve the desired effect. The feeding of the biological control agents is therefore crucial in the breeding process envisaged.

A large number of documents describe methods for mass rearing biological control agents. Conventionally, biological control agents are raised in the presence of a population of prey allowing their feeding, then released into the cultures. The prey populations used may be other prey than natural prey, for example, prey that is easier to breed if they allow the feeding of biological control agents. For example, in the field of mass rearing of arthropods as biological control agents, GB2393890 discloses that Amblyseius cucumeris may use Tyrophagus putrescentiae, T.tropicus and Acarus siro as surrogate preys. Some prey appear to have special benefits, and allow for better growth of several predator species. Thus, WO2008 / 015393 describes the use of Thyreophagus entomophagus as a food source for various species of predators, among which Amblyseius swirskii, Phytoseiulus persimilis, Amblyseius cucumeris, Amblyseius degenerans, Hypoaspis miles, Euseius tularensis, Typhlodromus occidentalis, Typhlodromus pyri, Amblysieus fallacies , Zetzellia mali, Amblyseius degenerans, Amblyseius aberrans, Amblyseius barkeri, Amblyseius andersoni, Amblyseius fallacis, Amblyseius finlandicus, Amblyseius lailae, Amblyseius limonicus, Amblyseius montdorensis, Amblyseius ovalis, Amblyseius scutalis, Amblyseius stipulatus and Amblyseius womersleyi.

The inventors have surprisingly discovered that the use of a population of Aleuroglyphus ovatus as a source of nutrients for breeding populations of predatory mites is particularly advantageous. In fact, the inventors have discovered that populations of predatory mites fed with Aleuroglyphus ovatus can grow more significantly than when these predators are fed with other astigmatics.

In addition, the populations of Aleuroglyphus ovatus have the advantage of being inexpensive to raise, the cost of the raw material and the energy required for breeding Aleuroglyphus ovatus is lower than for other astigmatics. Finally, Aleuroglyphus ovatus has the advantage of being a cosmopolitan species, the breeding of which is possible in any country.

detailed description

A first object of the invention is the use of a population of Aleuroglyphus ovatus as a source of nutrients for breeding a population of predatory mites.

A second subject of the invention is a biological control composition comprising:

- a nutrient source comprising or consisting of a population of Aleuroglyphus ovatus;

at least one predatory mite population, preferably selected from the list consisting of Neoseiulus cucumeris, Amblyseius largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius finlandicus, Euseius ovalis, Euseius scutalis, Euseius hibisci, Euseius stipulatus,

Galendromus occidentalis, Iphiseius degenerans, Robustulus macrocheles, Hypoaspis miles, Pergamasus quisquiliarum, Phytoseius finitimus, Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri, Typhlodromalus peregrinus; - optionally, a substrate for support and / or dissemination. Finally, the subject of the present invention is also a method for breeding a population of predatory mites, preferably chosen from the list consisting of Neoseiulus cucumeris, Amblyseius largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii, Neoseiulus californicus, Neoseiulus Fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius finlandicus, Euseius ovalis, Euseius scutalis, Euseius hibisci, Euseius stipulatus, Galendromus occidentalis, Iphiseius degenerans, Robustulus macrocheles, Hypoaspis miles, Pergamasus quisquiliarum, Phytoseius finitimus, Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri, Typhlodromalus peregrinus, from a population of Aleuroglyphus ovatus as a source of nutrients

More preferably, the predatory mite population, preferably selected from the list consisting of Neoseiulus cucumeris, Amblyseius largoensis, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius ovalis, Euseius scutalis, Euseius hibisci, Macrocheles robustulus, Hypoaspis miles. Even more preferentially, the population of predatory mites is Neoseiulus cucumeris ,.

The term "nutrient source" according to the invention implies any embodiment in which the population of Aleuroglyphus ovatus is used to feed the predatory mites. The source of nutrients according to the invention is the only food or one of the foods used to feed the predatory mites. Preferably, the source of nutrients according to the invention is the only source of nutrients of animal origin. According to one embodiment, the source of nutrients according to the invention is the only food used to feed the population of predatory mites.

The nutrient source according to the invention comprises at least 10, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 98, 100% d. 'Aleuroglyphus ovatus, by weight relative to the total weight of the nutrient source. According to a preferred embodiment, the source of nutrients according to the invention consists of Aleuroglyphus ovatus.

Aleuroglyphus ovatus, also called brown-legged brown-legged, belongs to the class of Arachnida, the order Acari, the family Acaridae and the genus Aleuroglyphus. Aleuroglyphus ovatus is often found in large numbers in bran, wheat, flour and poultry feed. Aleuroglyphus ovatus has a white body, 0.48 to 0.67 mm long, brownish-brown legs and chelicer, and dorsal bristles longer than sce dorsal bristles.

According to a preferred embodiment, the population of Aleuroglyphus ovatus is composed of mobile Aleuroglyphus ovatus. Preferably, the population of Aleuroglyphus ovatus comprises at least 10, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 98, 100% d. 'Aleuroglyphus ovatus mobile. According to a particularly advantageous embodiment, the population of Aleuroglyphus ovatus consists of mobile Aleuroglyphus ovatus.

In order to determine if the Aleuroglyphus ovatus used are mobile, it is possible to proceed, for example, to the following test:

The population of Aleuroglyphus ovatus is arranged under a binocular loupe. 100 individuals are counted. Among these 100 individuals, the number of mobile individuals is determined. The ratio of the number of mobile Aleuroglyphus ovatus to the number of total Aleuroglyphus ovatus (Rm) is thus determined. According to one embodiment, the mites may be chosen for example from the families of phytoseiids (Phytoseiidae), Macrochelidae, Laelapidae or Cheyletidae.

Advantageously, the phytoseiides may be chosen from the subfamily Amblyseiinae, for example, Amblyseius largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii or from the genus Neoseiulus, eg Neoseiulus womersleyi, Neoseiulus californicus, Neoseiulus cucumeris, Neoseiulus fallacis, Neoseiulus longispinosus ; or among the genus Iphiseius, for example Iphiseius degenerans; or from the genus Galendromus, for example Galendromus occidentalis; or among the genus Iphiseius, for example Iphiseius degenerans; or from the genus Pergamasus, for example Pergamasus quisquiliarum; or among the genus Phytoseius, for example Phytoseius finitimus; or from the subfamily Typhlodrominae, in particular the genus Amblydromalus, for example Amblydromalus lailae, Amblydromalus limonicus or Amblydromalus manihoti; or among the genus Typhlodromus, for example Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri, or among the genus Typhlodromalus, for example Typhlodromalus peregrinus; or among the genus Typhlodromips, for example Typhlodromips montdorensis; or among the genus Euseius, for example Euseius ovalis, Euseius scutalis, Euseius finlandicus, Euseius gallicus, Euseius stipulatus, Euseius tularensis, Euseius hibisci. Advantageously, mites of the Macrochelidae family may be chosen from Macrocheles, for example Macrocheles robustulus. Advantageously, mites of the Laelapidae family may be chosen from Stratiolaelaps, Androlaelaps or Hypoaspis, for example Hypoaspis miles, or Hypoaspis scimitus.

Advantageously, the mites of the family Cheyletidae may be selected from the mites of the genus Cheyletus.

According to an advantageous embodiment, the use of a population of Aleuroglyphus ovatus as a source of nutrients for rearing a population of predatory mites is characterized in that the population of predatory mites is chosen from the group consisting of Neoseiulus cucumeris, Amblyseius largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius finlandicus, Euseius ovalis, Euseius scutalis, Euseius hibisci, Euseius stipulatus, Galendromus occidentalis, Iphiseius degenerans, Robustulus macrocheles, Hypoaspis miles, Pergamasus quisquiliarum, Phytoseius finitimus, Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri, Typhlodromalus peregrinus.

According to an even more advantageous embodiment, the use of a population of Aleuroglyphus ovatus as a source of nutrients for rearing a population of predatory mites is characterized in that the population of predatory mites is selected from the group consisting of Neoseiulus cucumeris, Amblyseius largoensis, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius ovalis, Euseius scutalis, Euseius hibisci, Macrocheles robustulus, Hypoaspis miles.

According to a particularly advantageous embodiment, the use of a population of Aleuroglyphus ovatus as a source of nutrients for breeding a population of predatory mites is characterized in that the population of predatory mites is constituted of mites of the species Neoseiulus cucumeris.

The inventors have surprisingly discovered that the results obtained using a population of Aleuroglyphus ovatus as a source of nutrients for breeding a population of predatory mites are particularly interesting when the ratio by weight of the population of Predatory mites on the population of Aleuroglyphus ovatus is between 1/1 and 1/10.

Thus, according to an advantageous embodiment of the invention, a population of Aleuroglyphus ovatus is used as a source of nutrients for breeding a population of predatory mites so that the weight ratio of the population of predatory mites on the population & Aleuroglyphus ovatus is between 1/2 and 1/7, preferentially between 1/3 and 1/5.

According to a particularly advantageous embodiment, a population of Aleuroglyphus ovatus comprising at least 10, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 , 98, 100% of mobile Aleuroglyphus ovatus, is used as a source of nutrients for breeding a population of predatory mites so that the weight ratio of the population of predatory mites to the population of Aleuroglyphus ovatus mobiles is between 1/2 and 1/7, preferably between 1/3 and 1/5. According to an advantageous embodiment, the biological control composition comprises:

at least one predatory mite population, preferably chosen from the list consisting of Neoseiulus cucumeris, Amblyseius largoensis,

Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius ovalis, Euseius scutalis, Euseius hibisci, Macrocheles robustulus, Hypoaspis miles;

- optionally, a substrate for support and / or dissemination.

According to a particular embodiment, the biological control composition comprises:

at least one predatory mite population, preferably selected from the list consisting of Neoseiulus cucumeris, Amblyseius largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius finlandicus,

Euseius ovalis, Euseius scutalis, Euseius hibisci, Euseius stipulatus, Galendromus occidentalis, Iphiseius degenerans, Macrocheles robustulus, Hypoaspis miles, Pergamasus quisquiliarum, Phytoseius finitimus, Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri, Typhlodromalus peregrinus;

- optionally, a substrate for support and / or dissemination,

and is characterized in that the weight ratio of the population of predatory mites to the Aleuroglyphus ovatus population is between 1/2 and 1/7, preferably between 1/3 and 1/5. According to a particularly advantageous embodiment, the biological control composition comprises:

at least one predatory mite population, preferably selected from the list consisting of Neoseiulus cucumeris, Amblyseius largoensis, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius ovalis, Euseius scutalis, Euseius hibisci, Macrocheles robustulus, Hypoaspis miles;

- optionally, a substrate for support and / or dissemination,

and is characterized in that the weight ratio of the population of predatory mites to the Aleuroglyphus ovatus population is between 1/2 and 1/7, preferably between 1/3 and 1/5.

Advantageously, the population of Aleuroglyphus ovatus of the composition according to the invention comprises at least 10, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 98, 100% of mobile Aleuroglyphus ovatus and the ratio by weight of the population of predatory mites on the population of mobile Aleuroglyphus ovatus is between 1/2 and 1/7, preferably between 1/3 and 1 / 5.

According to an advantageous embodiment, the composition of the invention comprises a single population of predatory mites.

According to one embodiment of the invention, a support or dissemination substrate is added to the mixture comprising the predatory mite population and the nutrient source. The support or dissemination substrate is selected from inert substrates, such as, for example, vermiculite particles, perlite particles, or mixtures thereof.

The composition according to the invention may further comprise traces of the rearing medium of the Aleuroglyphus ovatus population.

According to one embodiment, the subject of the invention is a method of breeding a population of predatory mites from a population of Aleuroglyphus ovatus, comprising the steps of:

a) collecting said population of Aleuroglyphus ovatus;

b) determining the weight of said population of Aleuroglyphus ovatus obtained; and

c) bringing into contact with said population of Aleuroglyphus ovatus and a population of predatory mites; characterized in that the weight ratio of the population of predatory mites to the population of Aleuroglyphus ovatus is between 1/2 and 1/7, preferably between 1/3 and 1/5. Figure 1: Fecundity of Amblyseius swirskii (mean number of eggs per female) according to the Astigmata mite provided as food, either A. ovatus or Tyrophagus putrescentiae.

Figure 2: Fertility to Amblyseius cucumeris (mean number of eggs per female) as a function of the Astigmata mite provided as food, either A. ovatus or Tyrophagus putrescentiae.

Figure 3: Longevity to 'Amblyseius swirskii as a function of the Astigmata mite provided as food, either A. ovatus or Tyrophagus putrescentiae.

Figure 4: Longevity to 'Amblyseius cucumeris as a function of the Astigmata mite provided as food, either A. ovatus or Tyrophagus putrescentiae.

The following examples are provided by way of illustration and are not, unless otherwise indicated, intended to be limiting.

Example 1

Young female Amblyseius swirskii from the same cohort were individually deposited in experimental cells at 25 ° C, 75% RH (Relative Humidity) and 16/8 L / D (for Light / Dark, ie 16 hours of day for 8 hours of night), and a male has been associated with each female. The following two feeding modalities were tested:

- a mixture of all stages of A. ovatus

- a mixture of all stages of T. putrescentiae

Fertility (average number of eggs / female) from 30 to 60 Amblyseius swirskii was then recorded every day between their first mating and their death, for each of the two conditions above.

The results are shown in Figure 1.

The longevity of these females was also noted for the two modalities indicated above, and is shown in Figure 3.

Conclusion Fecundity and longevity are higher when Amblyseius swirskii females are fed a mixture of all stages of A. ovatus than when fed with a mixture of all stages of T. putrescentiae.

Example 2

Young female Amblyseius cucumeris from the same cohort were individually deposited in experimental cells at 25 ° C, 75% RH and 16/8 (L / D), and one male was given to each female. The following two feeding modalities were tested:

- a mixture of all stages of A. ovatus

- a mixture of all stages of T. putrescentiae

The results are shown in Figure 2.

The longevity of these females was also noted for the two modalities indicated above, and is shown in Figure 4.

As was observed for Amblyseius swirskii in Example 1, fecundity and longevity are higher when Amblyseius cucumeris females are fed by a mixture of all stages of A. ovatus than when fed by a mixture of all stages of T. putrescentiae.

Claims

claims

1. Use of a population & Aleuroglyphus ovatus as a source of nutrients for rearing a population of predatory mites, characterized in that the population of predatory mites is selected from the list consisting of Neoseiulus cucumeris, Amblyseius Largoensis, Amblyseius andersoni, Amblyseius limonicus, Amblyseius womersleyii, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius finlandicus, Euseius ovalis, Euseius scutalis, Euseius hibisci, Euseius stipulatus, Galendromus occidentalis, Iphiseius degenerans, Macrocheles robustulus, Hypoaspis miles, Pergamasus quisquiliarum, Phytoseius finitimus, Typhlodromus athiasae, Typhlodromus doreenae, Typhlodromus exhilaratus, Typhlodromus phialatus, Typhlodromus pyri, Typhlodromalus peregrinus.

2. Use according to one of claims 1, characterized in that the population of predatory mites is chosen from the list consisting of:

Neoseiulus cucumeris, Amblyseius largoensis, Neoseiulus californicus, Neoseiulus fallacis, Neoseiulus longispinosus, Amblydromalus lailae, Typhlodromips montdorensis, Euseius ovalis, Euseius scutalis, Euseius hibisci, Macrocheles robustulus, Hypoaspis miles.

3. Use according to claim 2, characterized in that the population of predatory mites consists of mites of the species Neoseiulus cucumeris.

4. Biological control composition comprising:

- a nutrient source comprising a population of Aleuroglyphus ovatus;

- at least one population of predatory mites;

- optionally, a substrate for support and / or dissemination.

5. Composition according to claim 4, characterized in that the weight ratio of the population of predatory mites on the Aleuroglyphus ovatus population is between 1/3 and 1/5.

6. Composition according to one of claims 4 or 5, characterized in that the population of predatory mites is selected from the group consisting of

7. Composition according to Claim 6, characterized in that the population of predatory mites is made up of mites of the species Neoseiulus cucumeris.

A method of culturing a population of predatory mites from a population of Aleuroglyphus ovatus, comprising the steps of:

a) collecting said population of Aleuroglyphus ovatus;

c) bringing into contact with said population of Aleuroglyphus ovatus and a population of predatory mites;

characterized in that the weight ratio of the population of predatory mites to the population of Aleuroglyphus ovatus is between 1/3 and 1/5