WO2013182670A3 - Novel cell wall deconstruction enzymes of scytalidium thermophilum and uses thereof - Google Patents

Novel cell wall deconstruction enzymes of scytalidium thermophilum and uses thereof Download PDF

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Publication number
WO2013182670A3
WO2013182670A3 PCT/EP2013/061768 EP2013061768W WO2013182670A3 WO 2013182670 A3 WO2013182670 A3 WO 2013182670A3 EP 2013061768 W EP2013061768 W EP 2013061768W WO 2013182670 A3 WO2013182670 A3 WO 2013182670A3
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WO
WIPO (PCT)
Prior art keywords
seq
nos
nucleic acids
polypeptide
cell wall
Prior art date
Application number
PCT/EP2013/061768
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French (fr)
Other versions
WO2013182670A2 (en
Inventor
Adrian Tsang
Justin Powlowski
Gregory Butler
Original Assignee
Dsm Ip Assets B.V.
Concordia University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V., Concordia University filed Critical Dsm Ip Assets B.V.
Publication of WO2013182670A2 publication Critical patent/WO2013182670A2/en
Publication of WO2013182670A3 publication Critical patent/WO2013182670A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 62 and 64; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 62 and 64; c. a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 7, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61 and 63, or nucleic acids that are at least 60%, preferably at least 70%, more 2 preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 8, 59, 61 and 63;30 d. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set DSM IP Assets B.V. 28984-WO-PCT forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 7, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61 and 63; or e. a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50,52, 53, 55, 56, 58, 59, 61 and 63.
PCT/EP2013/061768 2012-06-08 2013-06-07 Novel cell wall deconstruction enzymes of scytalidium thermophilum and uses thereof WO2013182670A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261657082P 2012-06-08 2012-06-08
US61/657,082 2012-06-08

Publications (2)

Publication Number Publication Date
WO2013182670A2 WO2013182670A2 (en) 2013-12-12
WO2013182670A3 true WO2013182670A3 (en) 2014-03-20

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PCT/EP2013/061768 WO2013182670A2 (en) 2012-06-08 2013-06-07 Novel cell wall deconstruction enzymes of scytalidium thermophilum and uses thereof

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WO (1) WO2013182670A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103898079B (en) * 2014-04-16 2016-06-22 中国农业科学院饲料研究所 Temperature neutral xylanase XYN11B and gene and application in one

Citations (4)

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WO2001042433A2 (en) * 1999-12-07 2001-06-14 Danisco A/S Talaromyces emersonii xylanase
WO2009018537A2 (en) * 2007-08-02 2009-02-05 Dyadic International, Inc. Novel fungal enzymes
WO2012027374A2 (en) * 2010-08-23 2012-03-01 Dyadic International (Usa) Inc. Novel fungal carbohydrate hydrolases
WO2012027282A2 (en) * 2010-08-23 2012-03-01 Codexis, Inc. Recombinant lignocellulose degradation enzymes for the production of soluble sugars from cellulosic biomass

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DK0619947T3 (en) 1993-03-31 1997-10-20 Gist Brocades Nv Yeast formulation for making baked products
ATE202674T1 (en) 1993-12-24 2001-07-15 Dsm Nv DRY YEAST COMPOSITIONS
PE20010352A1 (en) 1999-08-17 2001-03-23 Dsm Nv LIQUID COMPOSITION TO IMPROVE BREAD
CA2421833A1 (en) 2000-09-28 2002-04-04 Dsm N.V. Liquid bread improving compositions
EP1345496A2 (en) 2000-12-20 2003-09-24 Dsm N.V. Liquid yeast compositions
EP1507857A1 (en) 2002-05-30 2005-02-23 DSM IP Assets B.V. Novel pectinases and uses thereof
WO2011000949A1 (en) 2009-07-03 2011-01-06 Dsm Ip Assets B.V. Talaromyces strains and enzyme compositions
JP2012533312A (en) 2009-07-22 2012-12-27 ディーエスエム アイピー アセッツ ビー．ブイ． Improved host cell for producing a compound of interest
AU2011214324B2 (en) 2010-02-11 2014-11-20 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001042433A2 (en) * 1999-12-07 2001-06-14 Danisco A/S Talaromyces emersonii xylanase
WO2009018537A2 (en) * 2007-08-02 2009-02-05 Dyadic International, Inc. Novel fungal enzymes
WO2012027374A2 (en) * 2010-08-23 2012-03-01 Dyadic International (Usa) Inc. Novel fungal carbohydrate hydrolases
WO2012027282A2 (en) * 2010-08-23 2012-03-01 Codexis, Inc. Recombinant lignocellulose degradation enzymes for the production of soluble sugars from cellulosic biomass

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
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DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, MOUCHACCA JEAN: "Thermophilic fungi: Biodiversity and taxonomic status", XP002714912, Database accession no. PREV199799654542 *
DATABASE EMBL [online] 19 February 1997 (1997-02-19), "Humicola grisea DNA for xylanase, complete cds.", XP002714909, retrieved from EBI accession no. EM_STD:AB001030 Database accession no. AB001030 *
DATABASE UniProt [online] 1 May 1997 (1997-05-01), "SubName: Full=Xylanase;", XP002714908, retrieved from EBI accession no. UNIPROT:P79046 Database accession no. P79046 *
FERREIRA VERONICA ET AL: "Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the beta-glucosidase gene", ELECTRONIC JOURNAL OF BIOTECHNOLOGY, vol. 13, no. 2, March 2010 (2010-03-01), XP002714910, ISSN: 0717-3458 *
IIKURA HIROSHI ET AL: "Cloning of a gene encoding a putative xylanase with a cellulose-binding domain from Humicola grisea", BIOSCIENCE BIOTECHNOLOGY BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, TOKYO, JAPAN, vol. 61, no. 9, 1 January 1997 (1997-01-01), pages 1593 - 1595, XP002154883, ISSN: 0916-8451 *
MANDALARI G ET AL: "Production of feruloyl esterases and xylanases by Talaromyces stipitatus and Humicola grisea var. thermoidea on industrial food processing by-products", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 99, no. 11, 1 July 2008 (2008-07-01), pages 5130 - 5133, XP022606333, ISSN: 0960-8524, [retrieved on 20071024], DOI: 10.1016/J.BIORTECH.2007.09.022 *
MORIYA REGINA Y ET AL: "Enzymatic bleaching of organosolv sugarcane bagasse pulps with recombinant xylanase of the fungus Humicola grisea and with commercial cartazyme HS xylanase", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 121, April 2005 (2005-04-01), pages 195 - 203, XP009173523, ISSN: 0273-2289 *
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