WO2013175428A3 - A genetically modified microorganism, a process and methods for production of isobutanol - Google Patents

A genetically modified microorganism, a process and methods for production of isobutanol Download PDF

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Publication number
WO2013175428A3
WO2013175428A3 PCT/IB2013/054274 IB2013054274W WO2013175428A3 WO 2013175428 A3 WO2013175428 A3 WO 2013175428A3 IB 2013054274 W IB2013054274 W IB 2013054274W WO 2013175428 A3 WO2013175428 A3 WO 2013175428A3
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WO
WIPO (PCT)
Prior art keywords
isobutanol
production
methods
genetically modified
modified microorganism
Prior art date
Application number
PCT/IB2013/054274
Other languages
French (fr)
Other versions
WO2013175428A2 (en
Inventor
Santanu Datta
Kadambi Sarangapani RAMANUJAN
Anand ANANDKUMAR
Original Assignee
Cellworks Research India Pvt. Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellworks Research India Pvt. Ltd filed Critical Cellworks Research India Pvt. Ltd
Publication of WO2013175428A2 publication Critical patent/WO2013175428A2/en
Publication of WO2013175428A3 publication Critical patent/WO2013175428A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present disclosure relates to an architecture of energy redistribution that can sustain the increased formation of cofactors like NADH/NADPH and key metabolites like pyruvate that are implicated in the production of isobutanol through biotransformation.
PCT/IB2013/054274 2012-05-23 2013-05-23 A genetically modified microorganism, a process and methods for production of isobutanol WO2013175428A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2068/CHE/2012 2012-05-23
IN2068CH2012 2012-05-23

Publications (2)

Publication Number Publication Date
WO2013175428A2 WO2013175428A2 (en) 2013-11-28
WO2013175428A3 true WO2013175428A3 (en) 2014-02-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2013/054274 WO2013175428A2 (en) 2012-05-23 2013-05-23 A genetically modified microorganism, a process and methods for production of isobutanol

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WO (1) WO2013175428A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021077455A1 (en) * 2019-10-24 2021-04-29 天津科技大学 Genetically engineered strain having high-yield of l-valine and method for producing l-valine by fermentation

Citations (3)

* Cited by examiner, † Cited by third party
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WO2010031772A2 (en) * 2008-09-16 2010-03-25 Dsm Ip Assets B.V. Alternative butanol production process in a microbial cell
WO2010051527A2 (en) * 2008-10-31 2010-05-06 Gevo, Inc. Engineered microorganisms capable of producing target compounds under anaerobic conditions
US20100209986A1 (en) * 2007-02-09 2010-08-19 The Regents Of The University Of California Biofuel production by recombinant microorganisms

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100209986A1 (en) * 2007-02-09 2010-08-19 The Regents Of The University Of California Biofuel production by recombinant microorganisms
WO2010031772A2 (en) * 2008-09-16 2010-03-25 Dsm Ip Assets B.V. Alternative butanol production process in a microbial cell
WO2010051527A2 (en) * 2008-10-31 2010-05-06 Gevo, Inc. Engineered microorganisms capable of producing target compounds under anaerobic conditions

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
ANTONINO BAEZ ET AL: "High-flux isobutanol production using engineered: a bioreactor study with in situ product removal", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 90, no. 5, 10 March 2011 (2011-03-10), pages 1681 - 1690, XP019902986, ISSN: 1432-0614, DOI: 10.1007/S00253-011-3173-Y *
ATSUMI SHOTA ET AL: "Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE (AND SUPPLEMENTARY INFORMATION), NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 451, no. 7174, 1 January 2008 (2008-01-01), pages 86 - 90, XP002491157, ISSN: 0028-0836, DOI: 10.1038/NATURE06450 *
C. T. TRINH ET AL: "Redesigning Escherichia coli Metabolism for Anaerobic Production of Isobutanol", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 77, no. 14, 15 July 2011 (2011-07-15), pages 4894 - 4904, XP055076038, ISSN: 0099-2240, DOI: 10.1128/AEM.00382-11 *
CHEN X ET AL: "Increased isobutanol production in Saccharomyces cerevisiae by overexpression of genes in valine metabolism", BIOTECHNOLOGY FOR BIOFUELS, BIOMED CENTRAL LTD, GB, vol. 4, no. 21, 28 July 2011 (2011-07-28), pages 1 - 12, XP002696693, ISSN: 1754-6834, DOI: 10.1186/1754-6834-4-21 *
EKATERINA A SAVRASOVA ET AL: "Use of the valine biosynthetic pathway to convert glucose into isobutanol", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY ; OFFICIAL JOURNAL OF THE SOCIETY FOR INDUSTRIAL MICROBIOLOGY, SPRINGER, BERLIN, DE, vol. 38, no. 9, 15 December 2010 (2010-12-15), pages 1287 - 1294, XP019943556, ISSN: 1476-5535, DOI: 10.1007/S10295-010-0907-2 *
JE-NIE PHUE ET AL: "Acetate accumulation through alternative metabolic pathways intriple mutant inB (BL21)", BIOTECHNOLOGY LETTERS, SPRINGER NETHERLANDS, DORDRECHT, vol. 32, no. 12, 12 August 2010 (2010-08-12), pages 1897 - 1903, XP019859060, ISSN: 1573-6776, DOI: 10.1007/S10529-010-0369-7 *
JIA JIAN ET AL: "Production of polyhydroxyalkanoates by Escherichia coli mutants with defected mixed acid fermentation pathways", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 87, no. 6, 10 June 2010 (2010-06-10), pages 2247 - 2256, XP019841704, ISSN: 1432-0614 *
KEVIN MICHAEL SMITH ET AL: "Engineering Corynebacterium glutamicum for isobutanol production", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 87, no. 3, 8 April 2010 (2010-04-08), pages 1045 - 1055, XP019841532, ISSN: 1432-0614 *
LIN ET AL: "Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: A study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 7, no. 5-6, 1 September 2005 (2005-09-01), pages 337 - 352, XP005207141, ISSN: 1096-7176, DOI: 10.1016/J.YMBEN.2005.06.002 *
PARK JIN HWAN ET AL: "Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 8 MAY 2007, vol. 104, no. 19, 8 May 2007 (2007-05-08), pages 7797 - 7802, XP002711681, ISSN: 0027-8424 *
SILLERS R ET AL: "Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 10, no. 6, 1 November 2008 (2008-11-01), pages 321 - 332, XP025800634, ISSN: 1096-7176, [retrieved on 20080803], DOI: 10.1016/J.YMBEN.2008.07.005 *
SINGH A ET AL: "Manipulating redox and ATP balancing for improved production of succinate in E. coli", METABOLIC ENGINEERING, ACADEMIC PRESS, US, vol. 13, no. 1, 1 January 2011 (2011-01-01), pages 76 - 81, XP027575828, ISSN: 1096-7176, [retrieved on 20101030] *

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