WO2013143057A1 - Quinazoline derivative and application thereof - Google Patents

Quinazoline derivative and application thereof Download PDF

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Publication number
WO2013143057A1
WO2013143057A1 PCT/CN2012/073051 CN2012073051W WO2013143057A1 WO 2013143057 A1 WO2013143057 A1 WO 2013143057A1 CN 2012073051 W CN2012073051 W CN 2012073051W WO 2013143057 A1 WO2013143057 A1 WO 2013143057A1
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Prior art keywords
quinazoline
isoxazole
methoxy
methylamino
dimethoxy
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PCT/CN2012/073051
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French (fr)
Chinese (zh)
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卢灿忠
雍建平
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中国科学院福建物质结构研究所
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Priority to US14/241,067 priority Critical patent/US9193718B2/en
Priority to PCT/CN2012/073051 priority patent/WO2013143057A1/en
Priority to EP12872724.5A priority patent/EP2752413B1/en
Publication of WO2013143057A1 publication Critical patent/WO2013143057A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms

Definitions

  • the present invention relates to a novel structure of a oxazoline heterocycle-containing quinazoline derivative and a pharmaceutical composition containing the same, and to the use thereof, in particular, to an epidermal growth factor protein tyrosine kinase ( An EGFR-TK) active quinazoline compound or a pharmaceutical composition thereof, which is useful as a medicament for treating diseases such as tumors and cancers associated with protein tyrosine kinases.
  • An EGFR-TK epidermal growth factor protein tyrosine kinase
  • Epidermal growth factor (EGF) binding to the Epidermal Growth Factor Receptor (EGFR) activates tyrosine kinase activity and thus activates responses that lead to cell proliferation. Increased national expression and activity of EGFR can ultimately lead to uncontrolled cell division.
  • Epidermal growth factor receptor tyrosine kinase is the first discovered protein tyrosine kinase, widely distributed in the cell membrane of various tissues of human body, in most tumors (eg bladder cancer, non-small cell lung cancer) Overexpression in ovarian cancer, breast cancer, gastric cancer, esophageal cancer, etc., the intracellular region of EGFR has a binding site for adenosine triphosphate (ATP), and EGFR inhibitors can competitively bind to ATP binding sites, thereby inhibiting EGFR. Phosphorylation blocks the transmission of downstream signals, thereby inhibiting the growth, differentiation and metastasis of tumor cells.
  • Targeted treatment of tumors with EGFR receptors is one of the most active areas of research in cancer therapy today. Significant effects have also been achieved in clinical studies. Among them, the study of small molecular compounds with quinazoline as the mother core is the most prominent.
  • Patent Application Publication No. WO 03/082831 discloses 4-(2,3-dihaloanilino:)quinazoline compounds substituted at the 6-position with a heterocyclyloxy or heterocyclylmethoxy group, The compound is an erbB, especially an EGFR tyrosine kinase inhibitor. All of the above documents are incorporated herein by reference.
  • Small molecule anti-tumor drugs based on the EGFR receptor and quinazoline as the mother nucleus such as: gefitinib (Iressa:), erlotinib and lapatinib have been approved by the FDA for clinical use.
  • I have developed an erbinib-based EGFR receptor inhibitor, ectatinib (Kemena:), developed by Dr. King Yinxiang and Dr. Ding Liming.
  • Phase III clinical studies have shown that the efficacy is comparable to that of erlotinib and is safer.
  • the dosage and program are more suitable for Chinese people. It was approved by the China Food and Drug Administration in June 2011 for the treatment of advanced non-small cell lung cancer.
  • the invention is based on erlotinib and ectinib, introducing an isoxazole heterocycle into a quinazoline core, synthesizing a series of quinazoline compounds containing an isoxazole heterocycle, inhibiting epidermal growth factor protein tyrosine in vitro Acid kinase (EGFR-TK) activity indicates that the compound has strong anti-EGFR-TK activity and can be used as an anti-tumor drug or a lead compound.
  • EGFR-TK epidermal growth factor protein tyrosine in vitro Acid kinase
  • An object of the present invention is to provide an isoxazole-containing quinazoline compound represented by Formula 1.
  • Activity studies have shown that the compound has an inhibitory effect on EGFR-TK activity as an active ingredient.
  • R 2 are each independently selected from the group consisting of hydrogen, d-C 6 decyloxy, halo d-C 6 decyloxy, C ⁇ methoxy d-methoxy, C 3 -C 8 cyclodecyloxy, a C 3 -C 8 heterocyclic alkoxy group containing at least one hetero atom selected from N, 0, S;
  • Z is - ⁇ -, C(R 5 ) 2 , S or -0-, wherein is hydrogen or d ⁇ C 3 fluorenyl, R 5 is the same or different and is selected from hydrogen or C ⁇ Cs fluorenyl;
  • n is an integer from 0 to 5.
  • Z is -NH-, C3 ⁇ 4 or -0-;
  • n is preferably from 1 to 4, more preferably from 2 to 3.
  • Z is -NH- or -0- and R 2 is hydrogen, C "C 3 alkoxylate, C "C 3 alkoxy C "C 3 alkoxy;
  • the isoxazole ring is ortho or para to the isoxazole ring, more preferably 4-fluoro, 4-chloro, 2-chloro, 4-bromo, 2,4-dichloro, 4-methyl, 4-methoxy, Hydrogen, 4-trifluoromethyl or 2,4-dimethoxy.
  • the quinazoline compound represented by the formula (I) is more preferably selected from any one of the following compounds. 4-[(3-phenyl-isoxazol-5-yl)-methoxy-]-quinazoline;
  • the quinazoline compound represented by the formula ⁇ can be selected to form a pharmaceutically acceptable salt with a pharmaceutically acceptable acid, respectively.
  • pharmaceutically acceptable salts includes, but is not limited to, salts formed with inorganic acids, such as hydrochlorides, phosphates, diphosphates, hydrobromides, sulfates, sulfinates, nitrates, and Similar to salts; also includes salts with organic acids such as lactic acid, oxalic acid, malate, maleate, fumarate, tartrate, succinate, citrate, lactate, sulfonate , p-toluenesulfonate, 2-hydroxyethylsulfonate, benzoate, salicylate, stearate, trifluoroacetic acid or amino acid and stearate such as acetate, HOOC-(CH2 i-COOH wherein ⁇ is a salt of 0-4, and the like.
  • pharmaceutically acceptable salts includes, but is not
  • the present invention also provides a pharmaceutical composition
  • a pharmaceutical composition comprising the quinazoline compound of the above formula (I), or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable, inert , non-toxic excipients or carriers.
  • the present invention also provides a quinazoline compound of the above formula (I), or a pharmaceutically acceptable salt thereof, for use as a medicament, in particular for the treatment of inhibiting EGFR transient expression and/or activity A highly effective tumor drug.
  • the present invention also provides the use of a quinazoline compound represented by the formula of any of the above, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for use in antitumor or cancer.
  • the tumor or cancer is a cancer that is transiently expressed and/or active with EGFR. More preferably, the tumor or cancer is selected from the group consisting of: bladder cancer, non-small cell lung cancer, ovarian cancer, breast cancer, gastric cancer, esophageal cancer, lung cancer, head and neck cancer, colon cancer, pharyngeal cancer, and pancreatic cancer, etc. Application in cell lung cancer.
  • the present invention also provides a quinazoline compound represented by the formula (X) of any of the preceding claims and/or pharmaceutically acceptable
  • the accepted salts are in the preparation of inhibitors that inhibit the transient expression and/or activity of EGFR.
  • the present invention also provides a method for producing an isoxazole heterocyclic-containing quinazoline compound represented by the formula ⁇ , characterized in that the method comprises the following steps:
  • 6,7-disubstituted 4-chloro-quinazoline (formula II) and 3-substituted phenyl-5-hydroxymethyl-isoxazole (formula III) or 3-substituted phenyl-5-aminomethyl - Isoxazole (Formula IV) is a starting material which is prepared by reaction in a dry organic solvent and a basic acid binding agent system.
  • the reaction temperature is from -20 ° C to reflux conditions, preferably from room temperature to reflux conditions.
  • the organic solvent is benzene, toluene, xylene, dichloromethane, chloroform, isopropanol, tetrahydrofuran or DMF, more preferably isopropanol.
  • the basic acid binding agent is an organic base or an inorganic base
  • the organic base is preferably triethylamine, tripropylamine, DMAP, potassium t-butoxide or the like
  • the inorganic base is preferably potassium carbonate or sodium hydride. Carbon Sodium and so on.
  • a preferred acid binding agent is triethylamine.
  • the intermediate 6,7-disubstituted 4-chloro-quinazoline of the formula ( ⁇ ) can be produced by the following method:
  • the intermediate 3-substituted phenyl-5-hydroxymethyl-isoxazole of formula (III) or the intermediate 3-substituted phenyl-5-aminomethyl-isoxine of formula (IV) can be prepared by the following method:
  • the substituted benzylformaldehyde is used as a raw material, and is prepared by synthesizing ruthenium, 1,3-dipolar cycloaddition reaction, methanesulfonyl esterification reaction, azidation, reduction reaction as described above:), specifically as follows:
  • Z is another substituent such as CH 2 , S
  • it can be prepared by using the corresponding propynyl chloride, propargyl mercaptan.
  • the compounds of formula I according to the invention include, but are not limited to, their optical isomers, racemates and mixtures thereof.
  • the C 3 -C 8 cyclodecyl group in the C 3 -C 8 cyclodecyloxy group of the present invention may be a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, a cycloheptyl group, a cyclooctyl group, and preferably It is a cyclopropyl group, a cyclopentyl group or a cyclohexyl group.
  • the C 3 -C 8 heterocyclic fluorenyl group may be piperazinyl, piperidinyl, Polinyl, pyrrolidinyl, homopiperazinyl, preferably piperazinyl, morpholinyl or piperidinyl.
  • an effective amount refers to an amount of the at least one compound and/or at least one pharmaceutically acceptable salt that is effective to "treat" a disease or condition in an individual.
  • an effective amount can reduce the number of cancer or tumor cells; reduce the size of the tumor; inhibit or prevent the invasion of tumor cells into peripheral organs, for example, tumors spread into soft tissues or bones; inhibit or prevent tumor metastasis; Or prevent the growth of tumors; to some extent alleviate one or more symptoms associated with cancer; reduce morbidity and mortality; improve quality of life; or a combination of the above effects.
  • An effective amount can be an amount that reduces the symptoms of the disease by inhibiting EGFR activity.
  • the effects of in vivo experiments can be measured by assessing, for example, survival, time to disease progression (TTP), response rate (RR), duration of response, and/or quality of life. It has been recognized by the skilled person that the effective amount may vary with the route of administration, the dosage of the excipient, and the combination with other drugs.
  • the term "effective amount” can also mean a dose effective to inhibit overexpression and/or activity of EGFR by the at least one compound and/or at least one pharmaceutically acceptable salt thereof.
  • Tyrosine kinase (EGFR) was expressed by the insect baculovirus expression system and purified by affinity chromatography on a Ni-NTA column. The assay was in accordance with the experimental standard, and the kinase reaction substrate Poly(Glu, Tyr) 4:1 (Sigma). Anti-phosphotyrosine monoclonal antibody PY99 (Santa Cruz), horseradish peroxidase-labeled goat anti-mouse IgG (Calbiochem), ATP, DTT, OPD (Amresco), ELISA plate (Corning) Other reagents were commercially available analytical grades, which were not treated without prior treatment prior to use. Isopropanol was treated with dry molecular sieves prior to use.
  • Example 1 Synthesis of 6,7-dimethoxy-4-chloro-quinazoline
  • the mother liquid was washed with water, washed with a 5% sodium hydrogen carbonate solution, washed with water, dried over anhydrous sodium sulfate, and then evaporated under reduced pressure to give the crude product 5-methylsulfonic acid-3-phenyl-isoxazole-5- Methanol ester, yield 68.0%.
  • the crude product was directly subjected to a hydrazine reaction without purification.
  • the quinazoline compound represented by the formula (I) or a pharmaceutically acceptable salt thereof is used as an EGFR enzyme inhibitor, and the exemplified compounds are assayed for inhibition of EGFR enzyme activity by an enzyme-linked immunosorbent assay.
  • ATP solution diluted with reaction buffer (5 ⁇ at a final ATP concentration) and a test tyrosine kinase diluted in reaction buffer were added.
  • the total volume of the reaction was 10 (L. Negative control wells and enzyme-free control wells were also established.
  • Negative control OD value - no enzyme control well OD value The inhibitory activity against the EGFR enzyme of the compound represented by the formula (I) or its salt was measured by the above activity test method.

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Abstract

Provided is a quinazoline compound represented by Formula (I), or a pharmaceutically acceptable salt thereof. R1 and R2 are each independently selected from hydrogen, C1-C6 alkoxy, halogenated C1-C6 alkoxy, C1-C6 alkoxy C1-C6 alkoxy, and C3-C8 cycloalkyloxy, containing at least one C3-C8 heterocyclylalkoxy selected from N, O, and S heteroatoms; Z is -NR4-, C(R5)2, S or -O-, R4 is hydrogen or C1-C3 alkyl, and R5 is the same as or different from R4 and selected from hydrogen or C1-C3 alkyl; R3 is selected from hydrogen, halogen, C1-C6 alkyl, C1-C6 alkoxy, or halogenated C1-C6 alkyl; and n is an integer from 0 to 5. Further provided are the compound represented by Formula (I), a preparation method of the pharmaceutically acceptable salt thereof, and a medical application thereof. The compound restrains the EGFR-TK activity, and thus is capable of being applied as medicine for treating diseases such as tumors and cancers related to protein tyrosine kinase.

Description

喹唑啉衍生物及用途 技术领域  Quinazoline derivatives and uses thereof
本发明涉及一类结构新颖的含异噁唑杂环的喹唑啉衍生物及含该类衍生 物的药物组合物及其用途， 具体而言， 涉及具有抑制表皮生长因子蛋白酪氨 酸激酶 (EGFR-TK)活性的喹唑啉化合物或其药物组合物，该类物质可作为治疗 与蛋白酪氨酸激酶有关的肿瘤、 癌症等疾病的药物。 背景技术  The present invention relates to a novel structure of a oxazoline heterocycle-containing quinazoline derivative and a pharmaceutical composition containing the same, and to the use thereof, in particular, to an epidermal growth factor protein tyrosine kinase ( An EGFR-TK) active quinazoline compound or a pharmaceutical composition thereof, which is useful as a medicament for treating diseases such as tumors and cancers associated with protein tyrosine kinases. Background technique
表皮生长因子 （Epidermal Growth Factor, EGF ) 与表皮生长因子受体 (Epidermal Growth Factor Receptor, EGFR)结合可以激活酪氨酸激酶的活性， 并因此激活导致细胞增殖的反应。 EGFR的国度表达和活性增强可以最终导致 不可控的细胞分裂。  Epidermal growth factor (EGF) binding to the Epidermal Growth Factor Receptor (EGFR) activates tyrosine kinase activity and thus activates responses that lead to cell proliferation. Increased national expression and activity of EGFR can ultimately lead to uncontrolled cell division.
表皮生长因子受体酪氨酸激酶 (EGFR-TK)是最早发现的蛋白酪氨酸激酶， 广泛地分布于人体各组织的细胞膜上， 其在大多数肿瘤 (如： 膀胱癌、 非小细 胞肺癌、 卵巢癌、 乳腺癌、 胃癌、 食道癌等)中过表达， EGFR 的胞内区有三 磷酸腺苷 (ATP)的结合位点， EGFR抑制剂可以竞争性的与 ATP结合位点相结 合， 从而抑制 EGFR的磷酸化， 阻断下游信号的传导， 进而抑制肿瘤细胞的 生长、 分化和转移。 以 EGFR受体为靶点进行靶向治疗肿瘤是当今癌症治疗 中研究十分活跃的领域之一。 在临床研究中也已经取得了显著的疗效。 其中 以喹唑啉为母核的小分子化合物研究最为突出。  Epidermal growth factor receptor tyrosine kinase (EGFR-TK) is the first discovered protein tyrosine kinase, widely distributed in the cell membrane of various tissues of human body, in most tumors (eg bladder cancer, non-small cell lung cancer) Overexpression in ovarian cancer, breast cancer, gastric cancer, esophageal cancer, etc., the intracellular region of EGFR has a binding site for adenosine triphosphate (ATP), and EGFR inhibitors can competitively bind to ATP binding sites, thereby inhibiting EGFR. Phosphorylation blocks the transmission of downstream signals, thereby inhibiting the growth, differentiation and metastasis of tumor cells. Targeted treatment of tumors with EGFR receptors is one of the most active areas of research in cancer therapy today. Significant effects have also been achieved in clinical studies. Among them, the study of small molecular compounds with quinazoline as the mother core is the most prominent.
专利申请公开号 W096/33977、 W096/33978, W096/33979, WO96/33980, W096/33981、 WO97/30034、 WO97/30035、 W097/38994 , W098/13354、 WOOO/55141、 WOOO/56720、 WO02/41882, WO03/82290、 EP566226和 EP837063 公开了在 4-位上携带苯胺基取代和在 6-和 /或 7-位上携带取代基的某些喹唑啉 化合物， 具有受体酪氨酸激酶抑制活性。 上述所有文献引入本文作为参考。 Patent Application Publication Nos. W096/33977, W096/33978, W096/33979, WO96/33980, The addition of an anilino group at the 4-position is disclosed in W096/33981, WO97/30034, WO97/30035, W097/38994, W098/13354, WOOO/55141, WOOO/56720, WO02/41882, WO03/82290, EP566226 and EP837063. And certain quinazoline compounds carrying a substituent at the 6- and/or 7-position have receptor tyrosine kinase inhibitory activity. All of the above documents are incorporated herein by reference.
专利申请公开号 WO03/082831公开了在 6-位上被杂环基氧基或杂环基垸 氧基取代的 4-(2， 3-二卤代苯胺基：)喹唑啉化合物， 所述化合物是 erbB尤其是 EGFR酪氨酸激酶抑制剂。 上述所有文献引入本文作为参考。  Patent Application Publication No. WO 03/082831 discloses 4-(2,3-dihaloanilino:)quinazoline compounds substituted at the 6-position with a heterocyclyloxy or heterocyclylmethoxy group, The compound is an erbB, especially an EGFR tyrosine kinase inhibitor. All of the above documents are incorporated herein by reference.
基于 EGFR受体和以喹唑啉为母核的小分子抗肿瘤药物如： 吉非替尼 (易 瑞沙：)、厄罗替尼和拉帕替尼已相继被 FDA批准用于临床。我国王印祥博士和 丁列明博士研发的基于厄罗替尼的 EGFR受体抑制剂埃克替尼 (凯美纳：)，三期 临床研究表明其疗效和厄罗替尼相当， 安全性更好， 给药剂量和方案更适合 中国人， 已于 2011年 6月被中国食品药品监督管理局批准用于晚期非小细胞 肺癌的治疗。  Small molecule anti-tumor drugs based on the EGFR receptor and quinazoline as the mother nucleus such as: gefitinib (Iressa:), erlotinib and lapatinib have been approved by the FDA for clinical use. I have developed an erbinib-based EGFR receptor inhibitor, ectatinib (Kemena:), developed by Dr. King Yinxiang and Dr. Ding Liming. Phase III clinical studies have shown that the efficacy is comparable to that of erlotinib and is safer. The dosage and program are more suitable for Chinese people. It was approved by the China Food and Drug Administration in June 2011 for the treatment of advanced non-small cell lung cancer.
本发明基于厄罗替尼和埃克替尼， 将异噁唑杂环引入喹唑啉母核， 合成 了一系列含异噁唑杂环的喹唑啉化合物， 体外抑制表皮生长因子蛋白酪氨酸 激酶 (EGFR-TK)活性表明该类化合物具有较强的抑 EGFR-TK活性，可作为抗 肿瘤药物或者先导化合物。 发明内容  The invention is based on erlotinib and ectinib, introducing an isoxazole heterocycle into a quinazoline core, synthesizing a series of quinazoline compounds containing an isoxazole heterocycle, inhibiting epidermal growth factor protein tyrosine in vitro Acid kinase (EGFR-TK) activity indicates that the compound has strong anti-EGFR-TK activity and can be used as an anti-tumor drug or a lead compound. Summary of the invention
本发明的目的在于， 提供了如式①所示的含异噁唑杂环的喹唑啉化合物。 经过活性研究表明， 以该化合物作为活性成分具有抑制 EGFR-TK活性。  An object of the present invention is to provide an isoxazole-containing quinazoline compound represented by Formula 1. Activity studies have shown that the compound has an inhibitory effect on EGFR-TK activity as an active ingredient.
本发明通过如下技术方案实现：  The invention is achieved by the following technical solutions:
一种式 所示的喹唑啉化合物， 或其药学上可接受的盐
Figure imgf000004_0001
a quinazoline compound of the formula, or a pharmaceutically acceptable salt thereof
Figure imgf000004_0001
(I)  (I)
其中： 和 R2各自独立地选自氢， d~C6垸氧基， 卤代 d~C6垸氧基， C^ 垸氧基 d 垸氧基， C3-C8环垸氧基， 含有至少一个选自 N， 0， S杂 原子的 C3-C8杂环垸氧基； Wherein: and R 2 are each independently selected from the group consisting of hydrogen, d-C 6 decyloxy, halo d-C 6 decyloxy, C 垸 methoxy d-methoxy, C 3 -C 8 cyclodecyloxy, a C 3 -C 8 heterocyclic alkoxy group containing at least one hetero atom selected from N, 0, S;
Z为 -Ν -， C(R5)2, S或 -0-， 其中 为氢或 d~C3垸基， R5相同或不同， 选自氢或 C^Cs垸基； Z is -Ν -, C(R 5 ) 2 , S or -0-, wherein is hydrogen or d~C 3 fluorenyl, R 5 is the same or different and is selected from hydrogen or C^Cs fluorenyl;
选自氢、 卤素， C广 C6垸基、 d~C6垸氧基或卤代 d~C6垸基； n为 0-5 的整数。 It is selected from the group consisting of hydrogen, halogen, C-C 6 fluorenyl, d-C 6 decyloxy or halogenated d-C 6 fluorenyl; n is an integer from 0 to 5.
根据本发明的优选技术方案， 式 （I) 中：  According to a preferred technical solution of the present invention, in the formula (I):
和 为氢， C^Cs垸氧基， d~C6垸氧基 d~C6垸氧基， 含有至少一个 选自 N， 0， S杂原子的 C3-C8杂环垸氧基； And hydrogen, C^Cs methoxy, d~C 6 methoxy d~C 6 methoxy, containing at least one C 3 -C 8 heterocyclomethoxy group selected from N, 0, S heteroatoms;
Z为 -NH-, C¾或 -0-；  Z is -NH-, C3⁄4 or -0-;
选自氢、 氟、 氯、 溴、 甲基、 甲氧基或三氟甲基， n优选为 1-4， 更优 选 2-3。  It is selected from hydrogen, fluorine, chlorine, bromine, methyl, methoxy or trifluoromethyl, and n is preferably from 1 to 4, more preferably from 2 to 3.
根据本发明的更优选技术方案， 式 （I) 中：  According to a more preferred technical solution of the present invention, in the formula (I):
Z为 -NH-或 -0- 禾口 R2为氢， C「C3烧氧基， C「C3烧氧基 C「C3烧氧基； Z is -NH- or -0- and R 2 is hydrogen, C "C 3 alkoxylate, C "C 3 alkoxy C "C 3 alkoxy;
优选在异噁唑环的邻位或对位， 更优选为 4-氟、 4-氯、 2-氯、 4-溴、 2,4- 二氯、 4-甲基、 4-甲氧基、 氢、 4-三氟甲基或 2,4-二甲氧基。  Preferably, it is ortho or para to the isoxazole ring, more preferably 4-fluoro, 4-chloro, 2-chloro, 4-bromo, 2,4-dichloro, 4-methyl, 4-methoxy, Hydrogen, 4-trifluoromethyl or 2,4-dimethoxy.
根据本发明， 所述式 (I)所示的喹唑啉化合物更优选选自下述任一种化合 4-[(3-苯基-异噁唑 -5-基) -甲氧基 -]-喹唑啉； According to the invention, the quinazoline compound represented by the formula (I) is more preferably selected from any one of the following compounds. 4-[(3-phenyl-isoxazol-5-yl)-methoxy-]-quinazoline;
4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(2-氯苯基) -异噁唑 -5-基: |-甲氧基+喹唑啉；  4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氧基+喹唑啉；  4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methoxy+quinazoline;
4-{[3-(2,4-二氯苯基) -异噁唑 -5-基] -甲氧基+喹唑啉；  4-{[3-(2,4-dichlorophenyl)-isoxazole-5-yl]-methoxy+quinazoline;
4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氧基+喹唑啉；  4-{[3-(4-methylphenyl)-isoxazole-5-yl]-methoxy+quinazoline;
4-{[3-(4-甲氧基苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-[(3-苯基-异噁唑 -5-基) -甲氧基小喹唑啉；  6,7-dimethoxy-4-[(3-phenyl-isoxazole-5-yl)-methoxy small quinazoline;
6,7-二甲氧基 -4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二氯苯基) -异噁唑 -5-基] -甲氧基+喹唑啉； 6,7-dimethoxy-4-{[3-(2,4-dichlorophenyl)-isoxazole-5-yl]-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氧基+喹唑啉； 6,7-dimethoxy-4-{[3-(4-methylphenyl)-isoxazole-5-yl]-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲氧基苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉； 6,7-dimethoxy-4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氧基 - 喹唑啉；6,7-dimethoxy-4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methoxy-quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基: |-甲氧基+喹唑啉；6,7-dimethoxy-4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-[(3-苯基-异噁唑 -5-基) -甲氧基小喹唑啉； [6,7-bis(2-methoxyethoxy)]-4-[(3-phenyl-isoxazole-5-yl)-methoxy small quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉; [6,7-二 (2-甲氧乙氧基) ]-4-U3-(4-氯苯基) -异噁唑 -5-基： I-甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基 )]-4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: |-甲氧基 +喹唑 啉； [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-fluorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline; [6,7-bis(2-methoxyethoxy)]-4-U3-(4-chlorophenyl)-isoxazol-5-yl: I-methoxy+quinazoline; [6, 7-bis(2-methoxyethoxy)]-4-{[3-(2-chlorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline; [6,7 - bis(2-methoxyethoxy)]-4-{[3-(4-bromophenyl)-isoxazol-5-yl]-methoxy+quinazoline; [6,7-di (2-methoxyethoxy)]-4-{[3-(2,4-dichlorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氧基- } -喹唑 啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methylphenyl)-isoxazol-5-yl]-methoxy- }-quinazoline ;
[6,7-二 (2-甲氧乙氧基) ]-4-{ [3-(4-甲氧基苯基) -异噁唑 -5-基] -甲氧基- } -喹唑 啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methoxyphenyl)-isoxazol-5-yl]-methoxy-}-quinazoline Porphyrin
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基] -甲氧基- } -喹 唑啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-trifluoromethylphenyl)-isoxazol-5-yl]-methoxy-}-quin Oxazoline
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2,4-二甲氧基苯基) -异噁唑 -5-基] -甲氧基+ 喹唑啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dimethoxyphenyl)-isoxazol-5-yl]-methoxy+ quin Oxazoline
4-[(3-苯基-异噁唑 -5-基) -甲氨基 -]-喹唑啉；  4-[(3-phenyl-isoxazole-5-yl)-methylamino-]-quinazoline;
4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氨基+喹唑啉；  4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methylamino+quinazoline;
4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: |-甲氨基+喹唑啉；  4-{[3-(2,4-dichlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-甲基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-methylphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-甲氧基苯基) -异噁唑 -5-基: |-甲氨基+喹唑啉；  4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基： |-甲氨基+喹唑啉； 6,7-二甲氧基 -4-[(3-苯基-异噁唑 -5-基) -甲氨基小喹唑啉； 4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline; 6,7-dimethoxy-4-[(3-phenyl-isoxazol-5-yl)-methylaminosauline;
6,7-二甲氧基 -4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二氯苯基 异噁唑 -5-基: |-甲氧基+喹唑啉； 6,7-dimethoxy-4-{[3-(2,4-dichlorophenyl isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氨基+喹唑啉； 6,7-dimethoxy-4-{[3-(4-methylphenyl)-isoxazole-5-yl]-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲氧基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉； 6,7-dimethoxy-4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氨基 - 喹唑啉；6,7-dimethoxy-4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methylamino-quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基: |-甲氨基+喹唑啉；6,7-dimethoxy-4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-[(3-苯基-异噁唑 -5-基) -甲氨基小喹唑啉； [6,7-bis(2-methoxyethoxy)]-4-[(3-phenyl-isoxazol-5-yl)-methylaminosauline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-氟-苯基) -异噁唑 -5-基] -甲氨基+喹唑啉； [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-fluoro-phenyl)-isoxazole-5-yl]-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；[6,7-bis(2-methoxyethoxy)]-4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；[6,7-bis(2-methoxyethoxy)]-4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氨基+喹唑啉；[6,7-bis(2-methoxyethoxy)]-4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基 )]-4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: |-甲氨基 +喹唑 [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dichlorophenyl)-isoxazole-5-yl: |-methylamino + quinazoline
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氨基- } -喹唑 [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methylphenyl)-isoxazole-5-yl]-methylamino- }-quinazoline
[6,7-二 (2-甲氧乙氧基) ]-4-{ [3-(4-甲氧基苯基) -异噁唑 -5-基] -甲氨基- } -喹唑 [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methoxyphenyl)-isoxazole-5-yl]-methylamino- }-quinazoline
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基] -甲氨基- } -喹 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2,4-二甲氧基苯基) -异噁唑 -5-基] -甲氨基+ 喹唑啉。 [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-trifluoromethylphenyl)-isoxazol-5-yl]-methylamino- }-quin [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dimethoxyphenyl)-isoxazol-5-yl]-methylamino+ quinazole Porphyrin.
式 ω所示的喹唑啉化合物， 可以分别选择与药学上可接受的酸形成药学 上可接受的盐。 其中术语"药学上可接受的盐"包括但不限于与无机酸形成的 盐， 如盐酸盐、 磷酸盐、 二磷酸盐、 氢溴酸盐、 硫酸盐、 亚磺酸盐、 硝酸盐、 及其类似盐； 也包括与有机酸形成的盐， 如乳酸、 草酸、 苹果酸盐、 马来酸 盐、 富马酸盐、 酒石酸盐、 琥珀酸盐、 柠檬酸盐、 乳酸盐、 磺酸盐、 对甲苯 磺酸盐、 2-羟乙基磺酸盐、 苯甲酸盐、 水杨酸盐、 硬脂酸盐、 三氟乙酸或氨基 酸和链垸酸盐如醋酸盐， HOOC-(CH2 i-COOH其中 η是 0-4的盐， 及其类似 盐。 类似地， 药学上可接受的阳离子包括但不限于钠、 钾、 钙、 铝、 锂和铵。  The quinazoline compound represented by the formula ω can be selected to form a pharmaceutically acceptable salt with a pharmaceutically acceptable acid, respectively. Wherein the term "pharmaceutically acceptable salts" includes, but is not limited to, salts formed with inorganic acids, such as hydrochlorides, phosphates, diphosphates, hydrobromides, sulfates, sulfinates, nitrates, and Similar to salts; also includes salts with organic acids such as lactic acid, oxalic acid, malate, maleate, fumarate, tartrate, succinate, citrate, lactate, sulfonate , p-toluenesulfonate, 2-hydroxyethylsulfonate, benzoate, salicylate, stearate, trifluoroacetic acid or amino acid and stearate such as acetate, HOOC-(CH2 i-COOH wherein η is a salt of 0-4, and the like. Similarly, pharmaceutically acceptable cations include, but are not limited to, sodium, potassium, calcium, aluminum, lithium, and ammonium.
本发明还提供一种药物组合物， 其包括前述任一项的式 (I)所示的喹唑啉 化合物， 或其药学上可接受的盐， 以及至少一种药学上可接受的、 惰性的、 无毒的赋形剂或载体。  The present invention also provides a pharmaceutical composition comprising the quinazoline compound of the above formula (I), or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable, inert , non-toxic excipients or carriers.
本发明还提供用作药物的前述任一项的式 (I)所示的喹唑啉化合物或其药 学上可接受的盐， 尤其是一种用于治疗对于抑制 EGFR过渡表达和 /或活性过 高有效的肿瘤的药物。  The present invention also provides a quinazoline compound of the above formula (I), or a pharmaceutically acceptable salt thereof, for use as a medicament, in particular for the treatment of inhibiting EGFR transient expression and/or activity A highly effective tumor drug.
本发明还提供一种前述任一项的式 所示的喹唑啉化合物或其药学上可 接受的盐在制备用于抗肿瘤或癌症药物中的应用。  The present invention also provides the use of a quinazoline compound represented by the formula of any of the above, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for use in antitumor or cancer.
根据本发明， 所述的肿瘤或癌症是与 EGFR过渡表达和 /或活性过高的癌 症。 更优选地， 所述肿瘤或癌症选自： 膀胱癌， 非小细胞肺癌， 卵巢癌， 乳 腺癌， 胃癌， 食道癌， 肺癌， 头颈癌， 结肠癌， 咽癌， 和胰腺癌等， 更是非 小细胞肺癌中的应用。  According to the present invention, the tumor or cancer is a cancer that is transiently expressed and/or active with EGFR. More preferably, the tumor or cancer is selected from the group consisting of: bladder cancer, non-small cell lung cancer, ovarian cancer, breast cancer, gastric cancer, esophageal cancer, lung cancer, head and neck cancer, colon cancer, pharyngeal cancer, and pancreatic cancer, etc. Application in cell lung cancer.
本发明还提供一种前述任一项的式 (X)所示的喹唑啉化合物和 /或药学上可 接受的盐在制备抑制 EGFR的过渡表达和 /或活性过高的抑制剂。 The present invention also provides a quinazoline compound represented by the formula (X) of any of the preceding claims and/or pharmaceutically acceptable The accepted salts are in the preparation of inhibitors that inhibit the transient expression and/or activity of EGFR.
本发明还提供一种式 ω所示的含异噁唑杂环的喹唑啉化合物的制备方 法， 其特征在于， 所述方法包括如下歩骤：  The present invention also provides a method for producing an isoxazole heterocyclic-containing quinazoline compound represented by the formula ω, characterized in that the method comprises the following steps:
以 6,7-二取代 4-氯-喹唑啉（式 II)和 3-取代苯基 -5-羟甲基-异噁唑（式 III) 或 3-取代苯基 -5-氨甲基-异噁唑（式 IV)为原料， 在干燥的有机溶剂和碱性缚 酸剂体系中反应制备。  6,7-disubstituted 4-chloro-quinazoline (formula II) and 3-substituted phenyl-5-hydroxymethyl-isoxazole (formula III) or 3-substituted phenyl-5-aminomethyl - Isoxazole (Formula IV) is a starting material which is prepared by reaction in a dry organic solvent and a basic acid binding agent system.
Figure imgf000009_0001
Figure imgf000009_0001
如果需要， 可以将式 （II) 中的任何官能团予以保护。  Any functional group in formula (II) can be protected if desired.
并且其后， 如果有必要 （以任何次序）：  And then, if necessary (in any order):
( 1 ) 除去任何保护剂， 和  (1) remove any protective agent, and
(2) 形成式 I化合物的药学上可接受的盐。  (2) A pharmaceutically acceptable salt of a compound of formula I is formed.
根据本发明， 所述反应温度为 -20°C至回流条件， 优选室温至回流条件。 根据本发明， 所述有机溶剂为苯、 甲苯、 二甲苯、 二氯甲垸、 氯仿、 异 丙醇、 四氢呋喃或 DMF, 更优选异丙醇。  According to the invention, the reaction temperature is from -20 ° C to reflux conditions, preferably from room temperature to reflux conditions. According to the invention, the organic solvent is benzene, toluene, xylene, dichloromethane, chloroform, isopropanol, tetrahydrofuran or DMF, more preferably isopropanol.
根据本发明， 所述碱性缚酸剂为有机碱或无机碱， 所述有机碱优选为三 乙胺、 三丙胺、 DMAP、 叔丁醇钾等； 所述无机碱优选为碳酸钾、 氢化钠、 碳 酸钠等。 优选的缚酸剂为三乙胺。 According to the present invention, the basic acid binding agent is an organic base or an inorganic base, and the organic base is preferably triethylamine, tripropylamine, DMAP, potassium t-butoxide or the like; the inorganic base is preferably potassium carbonate or sodium hydride. Carbon Sodium and so on. A preferred acid binding agent is triethylamine.
根据本发明， 所述式 （Π) 的中间体 6,7-二取代 4-氯 -喹唑啉可通过如下 方法制备：  According to the present invention, the intermediate 6,7-disubstituted 4-chloro-quinazoline of the formula (Π) can be produced by the following method:
以 6,7-二取代 -喹唑啉酮为原料，在二氯亚砜或三氯氧磷体系中回流制备 (Ri， R2同前述所述 Prepared by refluxing 6,7-disubstituted-quinazolinone in dichlorosulfoxide or phosphorus oxychloride system (Ri, R 2 as described above)
Figure imgf000010_0001
Figure imgf000010_0001
式（V) 式 (II)  Formula (V) (II)
根据本发明， 所述式 （III) 的中间体 3-取代苯基 -5-羟甲基 -异噁唑或式 (IV) 的中间体 3-取代苯基 -5-氨甲基-异噁唑可通过如下方法制备：  According to the invention, the intermediate 3-substituted phenyl-5-hydroxymethyl-isoxazole of formula (III) or the intermediate 3-substituted phenyl-5-aminomethyl-isoxine of formula (IV) The azole can be prepared by the following method:
以取代苯甲醛为原料， 通过合成肟、 1,3-偶极环加成反应、 甲磺酰酯化 反应、 叠氮化、 还原反应制备 如上所述：)， 具体见下述流程：  The substituted benzylformaldehyde is used as a raw material, and is prepared by synthesizing ruthenium, 1,3-dipolar cycloaddition reaction, methanesulfonyl esterification reaction, azidation, reduction reaction as described above:), specifically as follows:
Figure imgf000010_0002
同时， 对于 Z为其他取代基， 例如 CH2， S时， 可采用相应的丙炔基氯， 丙炔硫醇进行制备。
Figure imgf000010_0002
Meanwhile, when Z is another substituent such as CH 2 , S, it can be prepared by using the corresponding propynyl chloride, propargyl mercaptan.
本发明所述式 I化合物， 包括但不限于： 它们的光学异构体， 外消旋体及 其混合物。  The compounds of formula I according to the invention include, but are not limited to, their optical isomers, racemates and mixtures thereof.
本发明所述的 C3-C8环垸氧基中的 C3-C8环垸基可以为环丙基， 环丁基， 环戊基， 环己基， 环庚基， 环辛基， 优选为环丙基， 环戊基或环己基。 The C 3 -C 8 cyclodecyl group in the C 3 -C 8 cyclodecyloxy group of the present invention may be a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, a cycloheptyl group, a cyclooctyl group, and preferably It is a cyclopropyl group, a cyclopentyl group or a cyclohexyl group.
本发明所述的含有至少一个选自 N, 0， S杂原子的〇3-〇8杂环垸氧基中， C3-C8杂环垸基可以为哌嗪基， 哌啶基， 吗啉基， 吡咯垸基， 高哌嗪基， 优选 为哌嗪基， 吗啉基或哌啶基。 In the 〇 3 -〇 8 heterocyclic oxime group containing at least one hetero atom selected from N, 0, S according to the present invention, the C 3 -C 8 heterocyclic fluorenyl group may be piperazinyl, piperidinyl, Polinyl, pyrrolidinyl, homopiperazinyl, preferably piperazinyl, morpholinyl or piperidinyl.
术语"有效量 "指的是， 所述至少一种化合物和 /或至少一种药学上可接受 的盐对于能有效"治疗"个体的一种疾病或不适的用量。如果是癌症时，有效量 能减少癌症或肿瘤细胞的数目； 缩小肿瘤的大小； 抑制或阻止肿瘤细胞向周 边器官的侵入， 例如， 肿瘤蔓延入软组织或骨骼中； 抑制或阻止肿瘤的转移； 抑制或阻止肿瘤的生长； 一定程度上减轻一种或多种与癌症相关的症状； 减 少发病率和死亡率； 提高生活质量； 或者是上述效果的结合。 有效量可以是 通过抑制 EGFR活性来减少疾病症状的用量。 对于癌症治疗， 体内实验的效 果可以通过评估如存活期、 疾病进展时间 (Time to DiseaseProgression, TTP)、 反应率 (Response Rates, RR), 持续反应期和 /或生活质量来测量。 专业人员已 经意识到， 有效量可以随着给药的途径、 赋形剂的剂量、 以及与其他药物的 合用而变化。  The term "effective amount" refers to an amount of the at least one compound and/or at least one pharmaceutically acceptable salt that is effective to "treat" a disease or condition in an individual. In the case of cancer, an effective amount can reduce the number of cancer or tumor cells; reduce the size of the tumor; inhibit or prevent the invasion of tumor cells into peripheral organs, for example, tumors spread into soft tissues or bones; inhibit or prevent tumor metastasis; Or prevent the growth of tumors; to some extent alleviate one or more symptoms associated with cancer; reduce morbidity and mortality; improve quality of life; or a combination of the above effects. An effective amount can be an amount that reduces the symptoms of the disease by inhibiting EGFR activity. For cancer treatment, the effects of in vivo experiments can be measured by assessing, for example, survival, time to disease progression (TTP), response rate (RR), duration of response, and/or quality of life. It has been recognized by the skilled person that the effective amount may vary with the route of administration, the dosage of the excipient, and the combination with other drugs.
术语"有效量"还可指得是所述至少一种化合物和 /或其至少一种药学上可 接受的盐对抑制 EGFR的过度表达和 /或活性过高有效的剂量。 具体实施方式 The term "effective amount" can also mean a dose effective to inhibit overexpression and/or activity of EGFR by the at least one compound and/or at least one pharmaceutically acceptable salt thereof. detailed description
下面结合实施例对本发明作进一歩的说明。 需要说明的是， 下述实施例 不能作为对本发明保护范围的限制， 任何在本发明基础上做出的改进都不违 背本发明的精神。  The present invention will be further described below in conjunction with the embodiments. It should be noted that the following embodiments are not intended to limit the scope of the invention, and any improvements made on the basis of the invention are not inconsistent with the spirit of the invention.
其中， 中间体和目标化合物的合成过程均以实施例中的代表说明， 其余 的中间体和目标化合物的合成过程同代表化合物。  Wherein, the synthesis process of the intermediate and the target compound are illustrated by the representatives in the examples, and the synthesis process of the remaining intermediates and the target compound is the same as the representative compound.
仪器与试剂：  Instruments and reagents:
AVANCE III 核磁共振仪 (400MHz, DMSO-d6,TMS为内标)， 离子阱液质 连用仪 (DECAX-30000 LCQ Deca XP), Shimadzu FTIR-8400S (日本岛津制作所 生产：)， XT5数字显示显微熔点测定仪 (北京市科仪电光仪器厂制造， 温度未经 校正）， 可调波长式微孔板酶标仪 (Molecular Devies SPECTRAMAX190). AVANCE III NMR (400MHz, DMSO-d 6 , TMS for internal standard), Ion Trap LCADE (DECAX-30000 LCQ Deca XP), Shimadzu FTIR-8400S (Japan Shimadzu::), XT5 number Display microscopic melting point analyzer (manufactured by Beijing Keyi Electro-optical Instrument Factory, temperature uncorrected), adjustable wavelength microplate microplate reader (Molecular Devies SPECTRAMAX190).
酪氨酸激酶 (EGFR)利用昆虫杆状病毒表达系统表达， 用 Ni-NTA柱亲和 纯化得到，经检验符合实验标准，激酶反应底物 Poly(Glu,Tyr)4:1(Sigma 公司）， 抗磷酸化酪氨酸的单克隆抗体 PY99(Santa Cruz公司），辣根过氧化酶标记羊抗 鼠的 IgG(Calbiochem 公司）， ATP 、DTT 、OPD(Amresco公司），酶标板 (Corning 公司 )， 其它试剂均为市售的分析纯， 在使用前没有特殊说明未进行处理， 异 丙醇在使用前用干燥分子筛处理。 实施例 1 6,7-二甲氧基 -4-氯-喹唑啉的合成 Tyrosine kinase (EGFR) was expressed by the insect baculovirus expression system and purified by affinity chromatography on a Ni-NTA column. The assay was in accordance with the experimental standard, and the kinase reaction substrate Poly(Glu, Tyr) 4:1 (Sigma). Anti-phosphotyrosine monoclonal antibody PY99 (Santa Cruz), horseradish peroxidase-labeled goat anti-mouse IgG (Calbiochem), ATP, DTT, OPD (Amresco), ELISA plate (Corning) Other reagents were commercially available analytical grades, which were not treated without prior treatment prior to use. Isopropanol was treated with dry molecular sieves prior to use. Example 1 Synthesis of 6,7-dimethoxy-4-chloro-quinazoline
将 4.12 g (20 mmol) 6,7-二甲氧基-喹唑啉酮加入 500 mL单口圆低烧瓶中， 接着缓慢加入含有 1滴 DMF的重蒸的二氯亚砜中 120 mL, 回流反应， TLC 检测反应完成后，减压蒸除过量的二氯亚砜，剩余物用 300 mL乙酸乙酯溶解， 用饱和的碳酸氢钠溶液洗至中性， 有机层无水硫酸钠干燥， 浓缩后柱分离 ( 5 油醚： 乙酸乙酷： 4： 1-2： 1)即得 6,7-二甲氧基 -4-氯-喹唑啉， 产率： 85%， JH NMR(DMSO-i 6, 400MHz): 4.01(s, 6H, 2CH3), 7.38(s, 1H), 7.45(s, 1H), 8.88(s,lH); ESI-MS(100%): 224([M]+, 100). 4.12 g (20 mmol) of 6,7-dimethoxy-quinazolinone was added to a 500 mL single-mouth round low flask, followed by slowly adding 120 mL of re-distilled thionyl chloride containing 1 drop of DMF. After the completion of the TLC reaction, the excess of thionyl chloride was evaporated under reduced pressure. The residue was dissolved in ethyl acetate (300 mL), washed with saturated sodium hydrogen carbonate solution to neutral, dried over anhydrous sodium sulfate and concentrated. Column separation ( 5 Oleic ether: Ethyl acetate: 4: 1-2: 1) 6,7-dimethoxy-4-chloro-quinazoline, yield: 85%, J H NMR (DMSO-i 6 , 400 MHz ): 4.01 (s, 6H, 2CH 3 ), 7.38 (s, 1H), 7.45 (s, 1H), 8.88 (s, lH); ESI-MS (100%): 224 ([M] + , 100) .
实施例 2 中间体 3-取代苯基 -5-羟甲基-异噁唑及 3-取代苯基 -5-氨甲基- 异噁唑的合成  Example 2 Synthesis of 3-substituted phenyl-5-hydroxymethyl-isoxazole and 3-substituted phenyl-5-aminomethyl-isoxazole
其中以 为11作为示例：  Which takes 11 as an example:
( 1 ) 苯甲醛肟的合成  (1) Synthesis of benzaldehyde oxime
-CHO NH3OH · HCI , Na2CO: -C=NOH -CHO NH 3 OH · HCI , Na 2 CO : -C=NOH
H  H
25°C  25°C
10.0 mmol 苯甲醛溶解在 30 mL 30% CH3OH和 ¾0溶液中， 加入装有 磁力搅拌器的三角瓶中， 搅拌下加入 10.0 mmol盐酸羟氨， 等盐酸羟氨溶解 后缓慢加入 5.0 mmol干燥研细的碳酸钠。 室温反应， TLC检测反应完成后， 体系减压蒸除甲醇后， 加入 30 mL H2O， 二氯甲垸 (3x30 mL)萃取， 合并有机 层， 无水硫酸钠干燥有机层. 脱溶剂即得苯甲醛肟粗产物， 收率 86.2 %。 该 粗产物不用分离纯化直接进行下歩反应。 10.0 mmol of benzaldehyde was dissolved in 30 mL of 30% CH 3 OH and 3⁄40 solution, added to a triangular flask equipped with a magnetic stirrer, and 10.0 mmol of hydroxylamine hydrochloride was added with stirring. After the hydroxylamine hydrochloride was dissolved, 5.0 mmol was slowly added. Fine sodium carbonate. After the reaction at room temperature, after the TLC reaction is completed, the system is evaporated to dryness under reduced pressure. Then, 30 mL of H 2 O and dichloromethane (3×30 mL) are added, and the organic layer is combined. The organic layer is dried over anhydrous sodium sulfate. The benzaldehyde oxime product was obtained in a yield of 86.2%. This crude product was directly subjected to a hydrazine reaction without isolation and purification.
(2) 3-苯基 -5-羟甲基-异噁唑  (2) 3-phenyl-5-hydroxymethyl-isoxazole
Figure imgf000013_0001
将 10.0 mmol苯甲醛肟和 30 mL干燥的 瓶中，搅拌下加入 1.60g (12.0 mmol) N-氯丁二酰亚胺（NCS) ,微微加热至 NCS 全部溶解后， 滴加 0.56 g 10.0 mmoi;)2-丙炔 -1-醇， 随后缓慢滴加入 20 mL含 10.1 g (10.0 mmol)三乙胺的二氯甲垸溶液，加完后体系回流. TLC检测反应完 成后， 母液水洗， 无水硫酸钠干燥， 柱分离 5:1-2： 1)即得 3- 苯基 -5-羟甲基-异噁唑， 收率 76.8 %。
Figure imgf000013_0001
10.0 mmol of benzaldehyde oxime and 30 mL of dried In a bottle, 1.60 g (12.0 mmol) of N-chlorosuccinimide (NCS) was added with stirring, and after slightly heating until the NCS was dissolved, 0.56 g of 10.0 mm oi;) 2-propyn-1-ol was added dropwise, followed by 20 mL of dichloromethane solution containing 10.1 g (10.0 mmol) of triethylamine was slowly added dropwise. After the addition, the system was refluxed. After the TLC reaction was completed, the mother liquor was washed with water and dried over anhydrous sodium sulfate. Column separation 5:1-2 : 1) 3-phenyl-5-hydroxymethyl-isoxazole was obtained in a yield of 76.8 %.
(3 ) 3-苯基 -5-氨甲基-异噁唑  (3) 3-phenyl-5-aminomethyl-isoxazole
Figure imgf000014_0001
将 10.0 mmol 5-羟甲基 -3-苯基-异噁唑和 30 mL干燥的二氯甲垸加入 250 mL单口圆底烧瓶中， 冰浴搅拌下将含 1.01g(10.0 mmol)三乙胺的 20 mL二 氯甲垸溶液加入体系中， 接着将溶有 1.37g (12.0 mmol) 甲磺酰氯 （MsCl) 的 5 mL二氯甲垸溶液缓慢滴加入体系中， 冰浴反应 2 h后， 室温反应。 TLC检 测反应完成后， 母液水洗， 5 %的碳酸氢钠溶液洗， 水洗， 无水硫酸钠干燥， 减压脱溶剂得粗产物 5-甲磺酸 -3-苯基-异噁唑 -5-甲醇酯， 收率 68.0 %。粗产物 不必纯化直接进行下歩反应。
Figure imgf000014_0001
Add 10.0 mmol of 5-hydroxymethyl-3-phenyl-isoxazole and 30 mL of dry dichloromethane to a 250 mL single-neck round bottom flask containing 1.01 g (10.0 mmol) of triethylamine in an ice bath with stirring. 20 mL of the solution of dichloromethane was added to the system, and then a solution of 1.37 g (12.0 mmol) of methanesulfonyl chloride (MsCl) in 5 mL of dichloromethane was slowly added dropwise to the system, and the reaction was carried out for 2 h in an ice bath. reaction. After the completion of the TLC reaction, the mother liquid was washed with water, washed with a 5% sodium hydrogen carbonate solution, washed with water, dried over anhydrous sodium sulfate, and then evaporated under reduced pressure to give the crude product 5-methylsulfonic acid-3-phenyl-isoxazole-5- Methanol ester, yield 68.0%. The crude product was directly subjected to a hydrazine reaction without purification.
将 5.0 mmol 5-甲磺酸 -3-苯基-异噁唑 -5-甲醇酯溶解在 20 mL干燥的 DMF 中， 加入 0.34 g ( 5.20 mmol)叠氮化钠， 室温搅拌溶解后， 置于 45 °C-50°C油 浴中反应， TLC指示反应完成后， 过滤， 滤饼用乙醚 （2x30mL) 洗涤， 合并 有机层， 向有机层中加 lOOmL水， 用乙醚（5x30mL)萃取， 合并有机层， 有 机层水洗 2次， 无水硫酸钠干燥， 脱溶剂后得粗产物 3-苯基 -5-叠氮甲基 -异噁 唑， 收率 90% ， 粗产物直接进行下面的还原反应. Dissolve 5.0 mmol of 5-phenyl-isoxazole-5-methanolsulfonate in 20 mL of dry DMF, add 0.34 g (5.20 mmol) of sodium azide, stir at room temperature, and place. The reaction was carried out in an oil bath at 45 ° C - 50 ° C. After TLC indicated that the reaction was completed, filtered, filtered, washed with diethyl ether (2×30 mL), and the organic layer was combined, and 100 mL of water was added to the organic layer and extracted with diethyl ether (5×30 mL). Layer, there The machine layer was washed twice with water and dried over anhydrous sodium sulfate. After desolvation, the crude product 3-phenyl-5-azidomethyl-isoxazole was obtained in a yield of 90%. The crude product was directly subjected to the following reduction.
5.0 mmol 5-叠氮甲基 -3-苯基-异噁唑溶解在 80 mL乙醇和 20 mL水的混合 溶液中，将 0.17g (2.6 mmol)的锌粉和 0.28g ( 5.2 mmol)的 N¾C1加入体系， 回流 lh， 真空脱去乙醇， 向体系加入 20mL水， 用 20% 的氢氧化钠溶液调至 pH值到 12， 向体系中加入 50mL DCM， 搅拌均匀后， 过滤， 滤渣在用少量的 水溶解， 过滤， 合并两次的滤液， 分出有机层， 有机层， 水洗， 无水硫酸钠 干燥， 真空脱溶剂， 残渣柱分离 二氣 ： V ™ ， 10： 1 ) 得产物 3-苯基 -5- 氨甲基-异噁唑， 收率 75% . 浅黄色固体， m.p: 39-40 。C , ^- MR (400MHz,CDCl3,TMS),5ppm: 1.60 ( s, 2H, NH2) , 3.91 (s, 2H, CH2), 6.40 (s, 1H), 7.39 (m, 2H, Ar-H), 7.76 (m, 2H, Ar-H) . 5.0 mmol 5-azidomethyl-3-phenyl-isoxazole was dissolved in a mixture of 80 mL ethanol and 20 mL water, 0.17 g (2.6 mmol) of zinc powder and 0.28 g (5.2 mmol) of N3⁄4C1 Add the system, reflux lh, remove ethanol from the vacuum, add 20mL water to the system, adjust the pH to 12 with 20% sodium hydroxide solution, add 50mL DCM to the system, stir evenly, filter, filter residue in a small amount The water is dissolved, filtered, and the filtrate is combined twice. The organic layer is separated, the organic layer is washed with water, dried over anhydrous sodium sulfate, evaporated in vacuo, and the residue is separated from the residue: V TM , 10: 1 ) -5- aminomethyl-isoxazole, yield 75%. Light yellow solid, mp: 39-40. C , ^- MR (400MHz, CDCl 3 , TMS), 5ppm: 1.60 ( s, 2H, NH 2 ) , 3.91 (s, 2H, CH 2 ), 6.40 (s, 1H), 7.39 (m, 2H, Ar -H), 7.76 (m, 2H, Ar-H).
实施例 3  Example 3
[6,7-二 (甲氧乙氧基 )]-4-[(3-苯基-异噁唑 -5-基) -甲氧基-] -喹唑啉  [6,7-bis(methoxyethoxy)]-4-[(3-phenyl-isoxazole-5-yl)-methoxy-]-quinazoline
Figure imgf000015_0001
Figure imgf000015_0001
将 0.3 g (lmmol)的 [6,7-二（甲氧乙氧基） ]-4-氯-喹唑啉溶解在 5 mL干燥的 异丙醇中，搅拌下将溶有 0.175g lmmoi;) 5-羟甲基 -3-苯基-异噁唑的 5 mL异丙 醇溶液缓慢滴加入反应体系， 接着加入 0.101 g lmmol)新蒸的三乙胺， 体系 室温搅拌 30 min后， 60°C反应， TLC检测反应完成后， 反应液真空浓缩， 残 渣直接柱分离 續)： =5:1-2:1 ) 即得目标化合物 [6,7-二 （甲氧乙氧 基） ]_4_{[3_(4_甲基-苯基) -异噁唑 _5-基] -甲氧基+喹唑啉 （下表 Q-15)。 其余 的化合物按照 [6,7-二（2-甲氧乙氧基） ]-4-{[3-(4-甲基-苯基) -异噁唑 -5-基] -甲氧 基- 喹唑啉的合成过程合成。 其结构均通过 IR, JH NMR, ESI-MS等分析方法 进行了表征。 优选出的化合物的物性常数及光谱数据以列表的形式进行说明： 优选出的化合物的结构、 编号及名称如下表所示： 表 1-优选化合物 0.3 g (1 mmol) of [6,7-bis(methoxyethoxy)]-4-chloro-quinazoline was dissolved in 5 mL of dry isopropanol and dissolved with 0.175 g of lmmoi;) A solution of 5-hydroxymethyl-3-phenyl-isoxazole in 5 mL of isopropanol was slowly added dropwise to the reaction system, followed by the addition of 0.101 g of 1 mmol of freshly distilled triethylamine. The system was stirred at room temperature for 30 min, 60 ° C. After the reaction is completed by TLC, the reaction solution is concentrated in vacuo, and the residue is Slag direct column separation): =5:1-2:1) The target compound [6,7-bis(methoxyethoxy)]_ 4 _{[ 3 _(4_methyl-phenyl) -Isooxazol-5-yl]-methoxy+quinazoline (Q-15 below). The remaining compound was [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methyl-phenyl)-isoxazol-5-yl]-methoxy- Synthesis of the quinazoline synthesis process. The structures were characterized by IR, J H NMR, ESI-MS and other analytical methods. The physical property constants and spectral data of the preferred compounds are illustrated in the form of a list: The structures, numbers and names of the preferred compounds are shown in the following table: Table 1 - Preferred compounds
Figure imgf000016_0001
N H Q-8 4-[(3-苯基-异噁唑 -5-基) -甲氨基 H -]—喹唑啉
Figure imgf000016_0001
NH Q-8 4-[(3-Phenyl-isoxazol-5-yl)-methylaminoH-]-quinazoline
4-CH3 Q-9 4-{[3-(4-甲基-苯基） -异噁唑 -5- 基] -甲氨基小喹唑啉  4-CH3 Q-9 4-{[3-(4-methyl-phenyl)-isoxazole-5-yl]-methylaminosodium quinazoline
4-OCH3 Q-10 4-{[3-(4-甲氧基-苯基） -异噁唑  4-OCH3 Q-10 4-{[3-(4-methoxy-phenyl)-isoxazole
-5-基] -甲氨基小喹唑啉  -5-yl]-methylaminosodium quinazoline
2-C1 Q-11 4-{[3-(2-氯-苯基)-异噁唑 -5-基] - 甲氨基 喹唑啉  2-C1 Q-11 4-{[3-(2-chloro-phenyl)-isoxazole-5-yl]-methylaminoquinazoline
4-C1 Q-12 4-{[3-(4-氯-苯基)-异噁唑 -5-基] - 甲氨基 喹唑啉  4-C1 Q-12 4-{[3-(4-Chloro-phenyl)-isoxazole-5-yl]-methylaminoquinazoline
2,4-二氯 Q-13 4-{[3-(2,4-二氯-苯基)-异噁唑 -5- 基] -甲氨基小喹唑啉  2,4-dichloro Q-13 4-{[3-(2,4-dichloro-phenyl)-isoxazole-5-yl]-methylaminosodium quinazoline
4-Br Q-14 4-{[3-(4-溴-苯基)-异噁唑 -5-基] - 甲氨基 喹唑啉  4-Br Q-14 4-{[3-(4-bromo-phenyl)-isoxazole-5-yl]-methylaminoquinazoline
H Q-15 6,7-二甲氧基 -4-[(3-苯基-异噁唑  H Q-15 6,7-dimethoxy-4-[(3-phenyl-isoxazole)
-5-基;) -甲氧基 -]-喹唑啉  -5-yl;) -methoxy-]-quinazoline
4-CH3 Q-16 6,7-二甲氧基 -4-{[3-(4-甲基-苯 4-CH3 Q-16 6,7-dimethoxy-4-{[3-(4-methyl-benzene)
Ri=R2= 0 基;) -异噁唑 -5-基] -甲氧基 +喹唑 -OCH3 啉 Ri=R 2 = 0 base;) -isoxazol-5-yl]-methoxy+quinazoline-OCH3 porphyrin
4-OCH3 Q-17 6,7-二甲氧基 -4-{[3-(4-甲氧基- 苯基)-异噁唑 -5-基] -甲氧基小喹 唑啉  4-OCH3 Q-17 6,7-Dimethoxy-4-{[3-(4-methoxy-phenyl)-isoxazole-5-yl]-methoxysiuquinazoline
2-C1 Q-18 6,7-二甲氧基 -4-{[3-(2-氯-苯基;) - 异噁唑 -5-基]-甲氧基 -}-喹唑啉2-C1 Q-18 6,7-dimethoxy-4-{[3-(2-chloro-phenyl;) - Isoxazole-5-yl]-methoxy-}-quinazoline
4-C1 Q-19 6,7-二甲氧基 -4-{[3-(4-氯-苯基;) - 异噁唑 -5-基]-甲氧基 -}-喹唑啉4-C1 Q-19 6,7-Dimethoxy-4-{[3-(4-chloro-phenyl;)-isoxazole-5-yl]-methoxy-}-quinazoline
2,4-二氯 Q-20 6,7-二甲氧基 -4-{[3-(2,4-二氯-苯 基;) -异噁唑 -5-基] -甲氧基 +喹唑 啉 2,4-DichloroQ-20 6,7-dimethoxy-4-{[3-(2,4-dichloro-phenyl;)-isoxazol-5-yl]-methoxy+ Quinazoline
4-Br Q-21 6,7-二甲氧基 -4-{[3-(4-溴-苯基) - 异噁唑 -5-基]-甲氧基 -}-喹唑啉  4-Br Q-21 6,7-Dimethoxy-4-{[3-(4-bromo-phenyl)-isoxazol-5-yl]-methoxy-}-quinazoline
N H Q-22 6,7-二甲氧基 -4-[(3-苯基-异噁唑 H -5-基) -甲氨基-] -喹唑啉 N H Q-22 6,7-dimethoxy-4-[(3-phenyl-isoxazole H-5-yl)-methylamino-]-quinazoline
4-CH3 Q-23 6,7-二甲氧基 -4-{[3-(4-甲基-苯 基) -异噁唑 -5-基] -甲氨基 +喹唑 啉  4-CH3 Q-23 6,7-Dimethoxy-4-{[3-(4-methyl-phenyl)-isoxazole-5-yl]-methylamino + quinazoline
4-OCH3 Q-24 6,7-二甲氧基 -4-{[3-(4-甲氧基- 苯基)-异噁唑 -5-基] -甲氨基小喹 唑啉  4-OCH3 Q-24 6,7-Dimethoxy-4-{[3-(4-methoxy-phenyl)-isoxazole-5-yl]-methylaminosodium quinazoline
2-C1 Q-25 6,7-二甲氧基 -4-{[3-(2-氯-苯基;) - 异噁唑 -5-基]-甲氨基 -}-喹唑啉 2-C1 Q-25 6,7-Dimethoxy-4-{[3-(2-chloro-phenyl;)-isoxazole-5-yl]-methylamino-}-quinazoline
4-C1 Q-26 6,7-二甲氧基 -4-{[3-(4-氯-苯基;) - 异噁唑 -5-基]-甲氨基 -}-喹唑啉4-C1 Q-26 6,7-Dimethoxy-4-{[3-(4-chloro-phenyl;)-isoxazole-5-yl]-methylamino-}-quinazoline
2,4-二氯 Q-27 6,7-二甲氧基 -4-{[3-(2,4-二氯-苯 基) -异噁唑 -5-基] -甲氨基 +喹唑 啉 2,4-Dichloro Q-27 6,7-dimethoxy-4-{[3-(2,4-dichloro-phenyl)-isoxazol-5-yl]-methylamino+quinazoline Porphyrin
4-Br Q-28 6,7-二甲氧基 -4-{[3-(4-溴-苯基) - 异噁唑 -5-基]-甲氨基 -}-喹唑啉 4-Br Q-28 6,7-Dimethoxy-4-{[3-(4-bromo-phenyl)-isoxazol-5-yl]-methylamino-}-quinazoline
H Q-29 [6,7-二 (2-甲氧乙氧基) ]-4-[(3-苯 基-异噁唑 -5-基)- 甲氧基-] -喹唑 啉 H Q-29 [6,7-bis(2-methoxyethoxy)]-4-[(3-phenyl-isoxazole-5-yl)-methoxy-]-quinazoline
4-CH3 Q-30 6,7-二 (2-甲氧乙氧基) -4-{[3-(4- 4-CH3 Q-30 6,7-bis (2-methoxyethoxy)-4-{[3-(4-
Ri=R2= 0 甲基-苯基) -异噁唑 -5-基]- 甲氧 CH3OCH2C 基- 喹唑啉 Ri=R 2 = 0 methyl-phenyl)-isoxazole-5-yl]-methoxy CH 3 OCH 2 C-quinazoline
H20- 4-OCH3 Q-31 6,7-二 (2-甲氧乙氧基) -4-{[3-(4- 甲氧基 -苯基；)—异噁唑 -5-基]- 甲 氧基 喹唑啉 H 2 0- 4-OCH3 Q-31 6,7-bis(2-methoxyethoxy)-4-{[3-(4-methoxy-phenyl;)-isoxazole-5-yl ]-methoxy quinazoline
2-C1 Q-32 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2- 氯-苯基) -异噁唑 -5-基]- 甲氧基 —}—喹唑啉  2-C1 Q-32 [6,7-bis(2-methoxyethoxy)]-4-{[3-(2-chloro-phenyl)-isoxazol-5-yl]-methoxy —}—quinazoline
4-C1 Q-33 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4- 氯-苯基) -异噁唑 -5-基]- 甲氧基 —}—喹唑啉  4-C1 Q-33 [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-chloro-phenyl)-isoxazol-5-yl]-methoxy —}—quinazoline
2,4-二氯 Q-34 [6,7- 二 （2- 甲 氧 乙 氧 基)] -4-{[3-(2,4-二氯-苯基) -异噁 唑 -5-基]- 甲氧基 -)—喹唑啉  2,4-DichloroQ-34 [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dichloro-phenyl)-isoxazole-5- Methyl-)-quinazoline
4-Br Q-35 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4- 溴-苯基) -异噁唑 -5-基] - 甲氧基 —}—喹唑啉 4-Br Q-35 [6,7-bis(2-methoxyethoxy)]-4-{[3-(4- Bromo-phenyl)-isoxazol-5-yl]-methoxy-}-quinazoline
N H Q-36 [6,7-二 (2-甲氧乙氧基) ]-4-[(3-苯 H 基-异噁唑—5-基)— 甲氨基-] -喹唑 啉  N H Q-36 [6,7-bis(2-methoxyethoxy)]-4-[(3-phenyl H-isoxazole-5-yl)-methylamino-]-quinazoline
4-CH3 Q-37 6,7-二 (2-甲氧乙氧基) -4-{[3-(4- 甲基-苯基) -异噁唑 -5-基]- 甲氨 基- 喹唑啉  4-CH3 Q-37 6,7-bis(2-methoxyethoxy)-4-{[3-(4-methyl-phenyl)-isoxazol-5-yl]-methylamino-quin Oxazoline
4-OCH3 Q-38 6,7-二 (2-甲氧乙氧基) -4-{[3-(4- 甲氧基 -苯基；)—异噁唑 -5-基]- 甲 氧基 喹唑啉  4-OCH3 Q-38 6,7-bis(2-methoxyethoxy)-4-{[3-(4-methoxy-phenyl;)-isoxazol-5-yl]-methoxy Quinazoline
2-C1 Q-39 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2- 氯-苯基) -异噁唑 -5-基]- 甲氨基 —}—喹唑啉  2-C1 Q-39 [6,7-bis(2-methoxyethoxy)]-4-{[3-(2-chloro-phenyl)-isoxazol-5-yl]-methylamino- }—quinazoline
4-C1 Q-40 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4- 氯-苯基) -异噁唑 -5-基]- 甲氨基 —}—喹唑啉  4-C1 Q-40 [6,7-Di(2-methoxyethoxy)]-4-{[3-(4-chloro-phenyl)-isoxazol-5-yl]-methylamino- }—quinazoline
2,4-二氯 Q-41 [6,7- 二 （2- 甲 氧 乙 氧 基)] -4-{[3-(2,4-二氯-苯基) -异噁 唑 -5-基]- 甲氨基 喹唑啉 2,4-DichloroQ-41 [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dichloro-phenyl)-isoxazole-5- Methylaminoquinazoline
4-Br Q-42 [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4- 溴-苯基) -异噁唑 -5-基] - 甲氨基 —}—喹唑啉
Figure imgf000021_0001
4-Br Q-42 [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-bromo-phenyl)-isoxazol-5-yl]-methylamino- }—quinazoline
Figure imgf000021_0001
Figure imgf000022_0001
Figure imgf000022_0001
Figure imgf000023_0001
Figure imgf000023_0001
Figure imgf000024_0001
Figure imgf000024_0002
Figure imgf000024_0003
Figure imgf000024_0001
Figure imgf000024_0002
Figure imgf000024_0003
(m,2H), 7.98-8.0 l(m, 2H), 8.24-8.27 (m, IH), 8.88(s, IH). (m, 2H), 7.98-8.0 l(m, 2H), 8.24-8.27 (m, IH), 8.88(s, IH).
Q-2 2.36 (s,3H,CH3), 5.86 (s, 2H, CH2), 7.26(s, IH, isoxazole-H), 7.33(d, 2H, J=8.0Hz), Q-2 2.36 (s,3H,CH 3 ), 5.86 (s, 2H, CH 2 ), 7.26(s, IH, isoxazole-H), 7.33(d, 2H, J=8.0Hz),
7.71-7.74 (m, IH), 7.80 (d,2H, J=8.0Hz), 7.96-8.02 (m, 2H), 8.26 (d, IH, J=8.0Hz), 8.88 (s, IH).  7.71-7.74 (m, IH), 7.80 (d, 2H, J=8.0Hz), 7.96-8.02 (m, 2H), 8.26 (d, IH, J=8.0Hz), 8.88 (s, IH).
Q-3 3.82(s, 3H, OCH3), 5.85 (s, 2H, CH2),7.05-7.08(m, 2H), 7.24(s, IH, isoxazole-H), Q-3 3.82(s, 3H, OCH 3 ), 5.85 (s, 2H, CH 2 ), 7.05-7.08(m, 2H), 7.24(s, IH, isoxazole-H),
7.71-7.75 (m,lH), 7.79-7.86(m, 2H), 7.96-8.03 (m, 2H), 8.26(d, IH, J=8.0Hz), 8.87 (s, IH).  7.71-7.75 (m, lH), 7.79-7.86 (m, 2H), 7.96-8.03 (m, 2H), 8.26 (d, IH, J = 8.0 Hz), 8.87 (s, IH).
Q-4 5.91 (s, 2H, CH2), 7.19(s, IH, isoxazole-H), 7.48-7.56 (m, 2H), 7.63-7.65 (m, Q-4 5.91 (s, 2H, CH 2 ), 7.19(s, IH, isoxazole-H), 7.48-7.56 (m, 2H), 7.63-7.65 (m,
1H),7 .67-7.75 (m, 2H), 7.97-8.02 (m, 2H), 8.24(d, IH, J=8.0Hz), 8.87 (s, IH). 1H), 7.67-7.75 (m, 2H), 7.97-8.02 (m, 2H), 8.24 (d, IH, J = 8.0 Hz), 8.87 (s, IH).
Q-5 5.88 (s, 2H, CH2), 7.34(s, IH, isoxazole-H), 7.60(d, 2H, J=8.4Hz),7.74-7.76(m,lH), Q-5 5.88 (s, 2H, CH 2 ), 7.34(s, IH, isoxazole-H), 7.60(d, 2H, J=8.4Hz), 7.74-7.76(m,lH),
7.93-7.95 (m,2H), 7.98-8.01(m, 2H), 8.26(d, IH, J=7.6Hz), 8.87 (s, IH).  7.93-7.95 (m, 2H), 7.98-8.01 (m, 2H), 8.26 (d, IH, J = 7.6 Hz), 8.87 (s, IH).
Q-6 5.90 (s, 2H, CH2), 7.21 (s, IH, isoxazole-H), 7.58-7.61 (m,lH), 7.71-7.77 (m, 2H), Q-6 5.90 (s, 2H, CH 2 ), 7.21 (s, IH, isoxazole-H), 7.58-7.61 (m, lH), 7.71-7.77 (m, 2H),
7.86(m, IH), 7.96-8.03 (m, 2H), 8.24(d, IH, J=8.4Hz), 8.88 (s, IH).  7.86(m, IH), 7.96-8.03 (m, 2H), 8.24(d, IH, J=8.4Hz), 8.88 (s, IH).
Q-7 5.88 (s, 2H, CH2), 7.34 (s, IH, isoxazole-H), 7.60(d, 2H, J=8.4Hz),7.74-7.76(m, IH), Q-7 5.88 (s, 2H, CH 2 ), 7.34 (s, IH, isoxazole-H), 7.60 (d, 2H, J=8.4Hz), 7.74-7.76(m, IH),
7.93-7.95 (m,2H), 7.98-8.01(m, 2H), 8.26(d, IH, J=7.6Hz), 8.88(s, IH).  7.93-7.95 (m, 2H), 7.98-8.01 (m, 2H), 8.26 (d, IH, J = 7.6 Hz), 8.88 (s, IH).
Q-8 4.29(t, J=6.4Hz,lH, NH), 5.87-5.89 (m,2H,CH2), 7.32(s, IH, isoxazole-H), 7.51-7.54 Q-8 4.29 (t, J = 6.4 Hz, lH, NH), 5.87-5.89 (m, 2H, CH 2 ), 7.32 (s, IH, isoxazole-H), 7.51-7.54
(m, 3H), 7.71-7.76 (m,lH), 7.89-7.92 (m,2H), 7.98-8.0 l(m, 2H), 8.24-8.27 (m, IH), 8.88(s, IH).  (m, 3H), 7.71-7.76 (m, lH), 7.89-7.92 (m, 2H), 7.98-8.0 l(m, 2H), 8.24-8.27 (m, IH), 8.88(s, IH).
Q-9 2.36 (s,3H,CH3), 4.30(t, J=6.6Hz,lH, NH), 5.86-5.85 (m, 2H, CH2), 7.26(s, IH, isoxazole-H), 7.33(d, 2H, J=8.0Hz), 7.71-7.74 (m, IH), 7.80 (d,2H, J=8.0Hz), 7.96-8.02 (m, 2H), 8.26 (d, IH, J=8.0Hz), 8.88 (s, IH). Q-9 2.36 (s,3H,CH 3 ), 4.30 (t, J=6.6Hz, lH, NH), 5.86-5.85 (m, 2H, CH 2 ), 7.26(s, IH, isoxazole-H), 7.33(d, 2H, J=8.0Hz), 7.71-7.74 (m, IH), 7.80 (d, 2H, J=8.0Hz), 7.96-8.02 (m, 2H), 8.26 (d, IH, J= 8.0Hz), 8.88 (s, IH).
Q-10 3.82(s, 3H, OCH3), 4.3 l(t, J=6.6Hz,lH, NH), 5.85-5.87 (m, 2H, CH2),7.05-7.08(m, 2H), 7.24(s, IH, isoxazole-H), 7.71-7.75 (m,lH), 7.79-7.86(m, 2H), 7.96-8.03 (m, 2H), 8.26(d, IH, J=8.0Hz), 8.87 (s, IH). Q-10 3.82(s, 3H, OCH3), 4.3 l(t, J=6.6Hz, lH, NH), 5.85-5.87 (m, 2H, CH 2 ), 7.05-7.08(m, 2H), 7.24(s, IH, isoxazole-H), 7.71-7.75 (m, lH), 7.79-7.86 (m, 2H), 7.96-8.03 (m, 2H), 8.26 (d, IH, J=8.0 Hz), 8.87 (s, IH).
Q-ii 4.31(t, J=6.6Hz,lH, NH), 5.91-5.92 (m, 2H, CH2), 7.19(s, IH, isoxazole-H), 7.48-7.56 Q-ii 4.31 (t, J = 6.6 Hz, lH, NH), 5.91-5.92 (m, 2H, CH 2 ), 7.19 (s, IH, isoxazole-H), 7.48-7.56
(m, 2H), 7.63-7.65 (m, 1H),7 .67-7.75 (m, 2H), 7.97-8.02 (m, 2H), 8.24(d, IH, J=8.0Hz), 8.87 (s, IH).  (m, 2H), 7.63-7.65 (m, 1H), 7.67-7.75 (m, 2H), 7.97-8.02 (m, 2H), 8.24 (d, IH, J=8.0Hz), 8.87 (s , IH).
Q-12 4.30(t, J=6.6Hz,lH, NH), 5.88-5.89 (m, 2H, CH2), 7.34(s, IH, isoxazole-H), 7.60(d, Q-12 4.30 (t, J = 6.6 Hz, lH, NH), 5.88-5.89 (m, 2H, CH 2 ), 7.34 (s, IH, isoxazole-H), 7.60 (d,
2H, J=8.4Hz),7.74-7.76(m,lH), 7.93-7.95 (m,2H), 7.98-8.01(m, 2H), 8.26(d, IH, J=7.6Hz), 8.87 (s, IH).  2H, J=8.4Hz), 7.74-7.76(m,lH), 7.93-7.95 (m,2H), 7.98-8.01(m, 2H), 8.26(d, IH, J=7.6Hz), 8.87 (s , IH).
Q-13 4.29(t, J=6.6Hz,lH, NH), 5.90-5.91 (m, 2H, CH2), 7.21 (s, IH, isoxazole-H), Q-13 4.29 (t, J = 6.6 Hz, lH, NH), 5.90-5.91 (m, 2H, CH 2 ), 7.21 (s, IH, isoxazole-H),
7.58-7.61 (m,lH), 7.71-7.77 (m, 2H), 7.86(m, IH), 7.96-8.03 (m, 2H), 8.24(d, IH, J=8.4Hz), 8.88 (s, IH).  7.58-7.61 (m,lH), 7.71-7.77 (m, 2H), 7.86(m, IH), 7.96-8.03 (m, 2H), 8.24(d, IH, J=8.4Hz), 8.88 (s, IH).
Q-14 4.29(t, J=6.6Hz,lH, NH), 5.88-5.90 (m, 2H, CH2), 7.34 (s, IH, isoxazole-H), 7.60(d, Q-14 4.29 (t, J = 6.6 Hz, lH, NH), 5.88-5.90 (m, 2H, CH 2 ), 7.34 (s, IH, isoxazole-H), 7.60 (d,
2H, J=8.4Hz),7.74-7.76(m,lH), 7.93-7.95 (m,2H), 7.98-8.01(m, 2H), 8.26(d, IH, J=7.6Hz), 8.88(s, IH).  2H, J=8.4Hz), 7.74-7.76(m,lH), 7.93-7.95 (m,2H), 7.98-8.01(m, 2H), 8.26(d, IH, J=7.6Hz), 8.88(s , IH).
Q-15 3.95(s, 6H, 3CH3), 5.84(s, 2H, CH2), 7.24 (s, IH, isoxazole-H), 7.38(d, 2H, J=6.8Hz), Q-15 3.95(s, 6H, 3CH 3 ), 5.84(s, 2H, CH 2 ), 7.24 (s, IH, isoxazole-H), 7.38 (d, 2H, J=6.8Hz),
7.51-7.53 (m, 2H), 7.65-7.72(m, IH), 7.89-7.91(m, 2H), 8.70(s, IH).  7.51-7.53 (m, 2H), 7.65-7.72 (m, IH), 7.89-7.91 (m, 2H), 8.70 (s, IH).
Q-16 2.36(s, 3H, CH3), 3.97(s, 6H, 2CH3), 5.83(s, 2H, CH2), 7.23(s, IH, isoxazole-H), Q-16 2.36(s, 3H, CH 3 ), 3.97(s, 6H, 2CH 3 ), 5.83(s, 2H, CH 2 ), 7.23(s, IH, isoxazole-H),
7.33(d, 2H, J=8.0Hz), 7.37(d, 2H, J=6.0Hz), 7.79(d, 2H, J=8.0Hz), 8.70(s,lH).  7.33 (d, 2H, J = 8.0 Hz), 7.37 (d, 2H, J = 6.0 Hz), 7.79 (d, 2H, J = 8.0 Hz), 8.70 (s, lH).
Q-17 3.82(s,3H, OCH3),3.97( s, 6H, 2CH3), 5.82(s, 2H, CH2), 7.07(d, 2H, J=7.6Hz), 7.20(s, Q-17 3.82(s,3H, OCH 3 ), 3.97( s, 6H, 2CH 3 ), 5.82(s, 2H, CH 2 ), 7.07(d, 2H, J=7.6Hz), 7.20(s,
IH, isoxazole-H), 7.37(d, 2H, J=4.8Hz), 7.84(d, 2H, J=7.6Hz), 8.69(s, IH). IH, isoxazole-H), 7.37 (d, 2H, J = 4.8 Hz), 7.84 (d, 2H, J = 7.6 Hz), 8.69 (s, IH).
Q-18 3.97(s, 6H, 2CH3), 5.87(s, 2H, CH2), 7.16(s, IH, isoxazole-H), 7.37(m, 2H), Q-18 3.97(s, 6H, 2CH 3 ), 5.87(s, 2H, CH 2 ), 7.16(s, IH, isoxazole-H), 7.37(m, 2H),
7.48-7.56(m, 2H), 7.64-7.73(m,2H), 8.70(s, IH).  7.48-7.56 (m, 2H), 7.64-7.73 (m, 2H), 8.70 (s, IH).
Q-19 3.97(s, 6H,2CH3), 5.85(s, 2H, CH2), 7.30(s, IH, isoxazole-H), 7.37(d, 2H, J=6.4Hz),7.60(d, 2H, J=8.4Hz),7.94(d, 2H, J=8.8Hz), 8.69(s, 1H). Q-19 3.97(s, 6H, 2CH 3 ), 5.85(s, 2H, CH 2 ), 7.30(s, IH, isoxazole-H), 7.37(d, 2H, J = 6.4 Hz), 7.60 (d, 2H, J = 8.4 Hz), 7.94 (d, 2H, J = 8.8 Hz), 8.69 (s, 1H).
Q-20 3.91(s, 6H,2CH3), 5.87(s, 2H, CH2), 7.17(s, 1H, isoxazole-H), 7.37(m, Q-20 3.91(s, 6H, 2CH 3 ), 5.87(s, 2H, CH 2 ), 7.17(s, 1H, isoxazole-H), 7.37(m,
2H),7.58-7.60(m,lH), 7.76(d, 1H, J=8.4Hz), 7.84-7.85(m, 1H), 8.70(s,lH).  2H), 7.58-7.60 (m, lH), 7.76 (d, 1H, J = 8.4 Hz), 7.84-7.85 (m, 1H), 8.70 (s, lH).
Q-21 3.97 (s, 6H,2CH3), 5.84 (s, 2H, CH2), 7.29 (s, 1H, isoxazole-H), 7.37(d, 2H, J=8.0Hz), Q-21 3.97 (s, 6H, 2CH 3 ), 5.84 (s, 2H, CH 2 ), 7.29 (s, 1H, isoxazole-H), 7.37 (d, 2H, J=8.0Hz),
7.73 (d,lH, J=8.4Hz ), 7.86 (d, 1H, J=8.4Hz), 8.69 (s,lH).  7.73 (d, lH, J = 8.4 Hz), 7.86 (d, 1H, J = 8.4 Hz), 8.69 (s, lH).
Q-22 3.95(s, 6H, 3CH3), 4.29(t, J=6.6Hz,lH, NH), 5.84-5.85(m, 2H, CH2), 7.24 (s, 1H, isoxazole-H), 7.38(d, 2H, J=6.8Hz), 7.51-7.53 (m, 2H), 7.65-7.72(m, 1H), 7.89-7.91(m, 2H), 8.70(s, 1H). Q-22 3.95(s, 6H, 3CH 3 ), 4.29(t, J=6.6Hz, lH, NH), 5.84-5.85(m, 2H, CH 2 ), 7.24 (s, 1H, isoxazole-H), 7.38 (d, 2H, J = 6.8 Hz), 7.51-7.53 (m, 2H), 7.65-7.72 (m, 1H), 7.89-7.91 (m, 2H), 8.70 (s, 1H).
Q-23 2.36(s, 3H, CH3), 3.97(s, 6H, 2CH3), 4.29(t, J=6.6Hz,lH, NH), 5.83-5.85(m, 2H, Q-23 2.36(s, 3H, CH 3 ), 3.97(s, 6H, 2CH 3 ), 4.29(t, J=6.6Hz, lH, NH), 5.83-5.85(m, 2H,
CH2), 7.23(s, 1H, isoxazole-H), 7.33(d, 2H, J=8.0Hz), 7.37(d, 2H, J=6.0Hz), 7.79(d, 2H, J=8.0Hz), 8.70(s,lH). CH 2 ), 7.23(s, 1H, isoxazole-H), 7.33(d, 2H, J=8.0Hz), 7.37(d, 2H, J=6.0Hz), 7.79(d, 2H, J=8.0Hz) , 8.70(s,lH).
Q-24 3.82(s,3H, OCH3), 3.97( s, 6H, 2CH3), 4.29(t, J=6.6Hz,lH, NH), 5.82-5.83(m, 2H, Q-24 3.82(s,3H, OCH 3 ), 3.97( s, 6H, 2CH 3 ), 4.29(t, J=6.6Hz, lH, NH), 5.82-5.83(m, 2H,
CH2), 7.07(d, 2H, J=7.6Hz), 7.20(s, 1H, isoxazole-H), 7.37(d, 2H, J=4.8Hz), 7.84(d, 2H, J=7.6Hz), 8.69(s, 1H). CH 2 ), 7.07 (d, 2H, J = 7.6 Hz), 7.20 (s, 1H, isoxazole-H), 7.37 (d, 2H, J = 4.8 Hz), 7.84 (d, 2H, J = 7.6 Hz) , 8.69(s, 1H).
Q-25 3.97(s, 6H, 2CH3), 4.3 l(t, J=6.6Hz,lH, NH), 5.87-5.89(m, 2H, CH2), 7.16(s, 1H, isoxazole-H), 7.37(m, 2H), 7.48-7.56(m, 2H), 7.64-7.73(m,2H), 8.70(s, 1H). Q-25 3.97(s, 6H, 2CH 3 ), 4.3 l(t, J=6.6Hz, lH, NH), 5.87-5.89(m, 2H, CH 2 ), 7.16(s, 1H, isoxazole-H) , 7.37(m, 2H), 7.48-7.56(m, 2H), 7.64-7.73(m, 2H), 8.70(s, 1H).
Q-26 3.97(s, 6H,2CH3), 4.3 l(t, J=6.6Hz,lH, NH), 5.85-5.86(m, 2H, CH2), 7.30(s, 1H, isoxazole-H), 7.37(d, 2H, J=6.4Hz),7.60(d, 2H, J=8.4Hz),7.94(d, 2H, J=8.8Hz), 8.69(s, 1H). Q-26 3.97(s, 6H, 2CH 3 ), 4.3 l(t, J=6.6Hz, lH, NH), 5.85-5.86(m, 2H, CH 2 ), 7.30(s, 1H, isoxazole-H) , 7.37 (d, 2H, J = 6.4 Hz), 7.60 (d, 2H, J = 8.4 Hz), 7.94 (d, 2H, J = 8.8 Hz), 8.69 (s, 1H).
Q-27 3.91(s, 6H,2CH3), 4.3 l(t, J=6.6Hz,lH, NH), 5.87-5.88(m, 2H, CH2), 7.17(s, 1H, isoxazole-H), 7.37(m, 2H),7.58-7.60(m,lH), 7.76(d, 1H, J=8.4Hz), 7.84-7.85(m, 1H), 8.70(s,lH). Q-27 3.91(s, 6H, 2CH 3 ), 4.3 l(t, J=6.6Hz, lH, NH), 5.87-5.88(m, 2H, CH 2 ), 7.17(s, 1H, isoxazole-H) , 7.37 (m, 2H), 7.58-7.60 (m, lH), 7.76 (d, 1H, J = 8.4 Hz), 7.84-7.85 (m, 1H), 8.70 (s, lH).
Q-28 3.97 (s, 6H,2CH3), 4.3 l(t, J=6.6Hz,lH, NH), 5.84-5.85 (m, 2H, CH2), 7.29 (s, 1H, isoxazole-H), 7.37(d, 2H, J=8.0Hz), 7.73 (d,lH, J=8.4Hz ), 7.86 (d, 1H, J=8.4Hz), 8.69 (s,lH). Q-28 3.97 (s, 6H, 2CH 3 ), 4.3 l(t, J=6.6Hz, lH, NH), 5.84-5.85 (m, 2H, CH 2 ), 7.29 (s, 1H, Isoxazole-H), 7.37 (d, 2H, J = 8.0 Hz), 7.73 (d, lH, J = 8.4 Hz), 7.86 (d, 1H, J = 8.4 Hz), 8.69 (s, lH).
Q-29 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.26-4.33(m, 4H, 2CH2),5.83(s, 2H, Q-29 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.26-4.33(m, 4H, 2CH 2 ), 5.83(s, 2H,
CH2), 7.23 (s, 1H, isoxazole-H),7.42(d, 2H, J=12.0Hz),7.50-7.54(m, 3H),7.89-7.91(m, 2H),8.69(s,lH). CH 2 ), 7.23 (s, 1H, isoxazole-H), 7.42 (d, 2H, J = 12.0 Hz), 7.50-7.54 (m, 3H), 7.89-7.91 (m, 2H), 8.69 (s, lH) ).
Q-30 2.36(s, 3H, Ph-CH3),3.36(s, 6H, 2CH3),3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, Q-30 2.36(s, 3H, Ph-CH 3 ), 3.36(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H,
2CH2), 5.81 (s, 2H, CH2), 7.23 (s, 1H, isoxazole-H),7.31-7.35(m, 2H), 7.38-7.41(m, 2H), 7.77-7.79(m, 2H), 8.68(s,lH). 2CH 2 ), 5.81 (s, 2H, CH 2 ), 7.23 (s, 1H, isoxazole-H), 7.31-7.35(m, 2H), 7.38-7.41(m, 2H), 7.77-7.79(m, 2H ), 8.68(s,lH).
Q-31 3.34 (s, 6H, 2CH3), 3.72-3.76 (m, 4H, 2CH2), 3.81 (s, 3H, Ph-OCH3), 4.25-4.33 (m, Q-31 3.34 (s, 6H, 2CH 3 ), 3.72-3.76 (m, 4H, 2CH 2 ), 3.81 (s, 3H, Ph-OCH 3 ), 4.25-4.33 (m,
4H, 2CH2), 5.80 (s, 2H, CH2), 7.07(d, 2H, J=8.8 Hz), 7.20 (s, 1H, isoxazole-H), 7.41 (d, 2H, J=12.0Hz), 8.69 (s,lH). 4H, 2CH 2 ), 5.80 (s, 2H, CH 2 ), 7.07 (d, 2H, J = 8.8 Hz), 7.20 (s, 1H, isoxazole-H), 7.41 (d, 2H, J = 12.0 Hz) , 8.69 (s, lH).
Q-32 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.26-4.33(m, 4H, 2CH2), 5.86(s, 2H, Q-32 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.26-4.33(m, 4H, 2CH 2 ), 5.86(s, 2H,
CH2), 7.16(s, 1H, isoxazole-H), 7.40(d, 2H, J=12.8Hz), 7.49-7.65(m, 2H), 7.71-7.72(m, 2H), 8.69(s, 1H). CH 2 ), 7.16(s, 1H, isoxazole-H), 7.40(d, 2H, J=12.8Hz), 7.49-7.65(m, 2H), 7.71-7.72(m, 2H), 8.69(s, 1H ).
Q-33 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, 2CH2), 5.83(s, 2H, Q-33 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H, 2CH 2 ), 5.83(s, 2H,
CH2), 7.30(s, 1H, isoxazole-H),7.42(d, 2H, J=13.2Hz),7.58-7.60(m, 2H),7.92-7.94(m, 2H), 8.68(s, 1H). CH 2 ), 7.30 (s, 1H, isoxazole-H), 7.42 (d, 2H, J = 13.2 Hz), 7.58-7.60 (m, 2H), 7.92-7.94 (m, 2H), 8.68 (s, 1H) ).
Q-34 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, 2CH2), 5.86(s, 2H, Q-34 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H, 2CH 2 ), 5.86(s, 2H,
CH2), 7.18(s, 1H, isoxazole-H),7.40(d, 2H, J=11.2Hz),7.58-7.61(m, lH),7.74-7.76(m, lH),7.85-7.86(m,lH), 8.69(s, 1H). CH 2 ), 7.18 (s, 1H, isoxazole-H), 7.40 (d, 2H, J = 11.2 Hz), 7.58-7.61 (m, lH), 7.74-7.76 (m, lH), 7.85-7.86 (m , lH), 8.69 (s, 1H).
Q-35 3.36(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, 2CH2), 5.83(s, 2H, Q-35 3.36(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H, 2CH 2 ), 5.83(s, 2H,
CH2), 7.30(s, 1H, isoxazole-H),7.40(d, 2H, J=12.8Hz),7.72-7.74(m, 2H), 7.85-7.87(m, 2H), 8.68(s, 1H). Q-36 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.26-4.30(m, 4H, 2CH2), 4.3 l(t, J=6.6Hz,lH, NH), 5.83-5.85(m, 2H, CH2), 7.23 (s, 1H, isoxazole-H),7.42(d, 2H, J=12.0Hz),7.50-7.54(m, 3H),7.89-7.91(m, 2H),8.69(s,lH). CH 2 ), 7.30 (s, 1H, isoxazole-H), 7.40 (d, 2H, J = 12.8 Hz), 7.72-7.74 (m, 2H), 7.85-7.87 (m, 2H), 8.68 (s, 1H) ). Q-36 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.26-4.30(m, 4H, 2CH 2 ), 4.3 l(t, J=6.6Hz, lH, NH ), 5.83-5.85(m, 2H, CH 2 ), 7.23 (s, 1H, isoxazole-H), 7.42 (d, 2H, J = 12.0 Hz), 7.50-7.54 (m, 3H), 7.89-7.91 ( m, 2H), 8.69 (s, lH).
Q-37 2.36(s, 3H, Ph-CH3), 3.36(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, Q-37 2.36(s, 3H, Ph-CH 3 ), 3.36(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H,
2CH2), 4.34(t, J=6.6Hz,lH, NH), 5.81-5.83 (m, 2H, CH2), 7.23 (s, 1H, isoxazole-H),7.31-7.35(m, 2H), 7.38-7.41(m, 2H), 7.77-7.79(m, 2H), 8.68(s,lH).2CH 2 ), 4.34 (t, J = 6.6 Hz, lH, NH), 5.81-5.83 (m, 2H, CH 2 ), 7.23 (s, 1H, isoxazole-H), 7.31-7.35 (m, 2H), 7.38-7.41(m, 2H), 7.77-7.79(m, 2H), 8.68(s,lH).
Q-38 3.34 (s, 6H, 2CH3), 3.72-3.76 (m, 4H, 2CH2), 3.81 (s, 3H, Ph-OCH3), 4.25-4.33 (m, Q-38 3.34 (s, 6H, 2CH 3 ), 3.72-3.76 (m, 4H, 2CH 2 ), 3.81 (s, 3H, Ph-OCH 3 ), 4.25-4.33 (m,
4H, 2CH2), 4.34(t, J=6.6Hz,lH, NH), 5.80-5.82 (m, 2H, CH2), 7.07(d, 2H, J=8.8 Hz), 7.20 (s, 1H, isoxazole-H), 7.41 (d, 2H, J=12.0Hz), 8.69 (s,lH). 4H, 2CH 2 ), 4.34 (t, J = 6.6 Hz, lH, NH), 5.80-5.82 (m, 2H, CH 2 ), 7.07 (d, 2H, J = 8.8 Hz), 7.20 (s, 1H, Isoxazole-H), 7.41 (d, 2H, J=12.0Hz), 8.69 (s,lH).
Q-39 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.26-4.33(m, 4H, 2CH2), 4.35(t, Q-39 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.26-4.33(m, 4H, 2CH 2 ), 4.35(t,
J=6.6Hz,lH, NH), 5.86-5.88(m, 2H, CH2), 7.16(s, 1H, isoxazole-H), 7.40(d, 2H, J=12.8Hz), 7.49-7.65(m, 2H), 7.71-7.72(m, 2H), 8.69(s, 1H). J = 6.6 Hz, lH, NH), 5.86-5.88 (m, 2H, CH 2 ), 7.16 (s, 1H, isoxazole-H), 7.40 (d, 2H, J = 12.8 Hz), 7.49-7.65 (m) , 2H), 7.71-7.72(m, 2H), 8.69(s, 1H).
Q-40 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, 2CH2), 4.35(t, Q-40 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H, 2CH 2 ), 4.35(t,
J=6.6Hz,lH, NH), 5.83-5.85(m, 2H, CH2), 7.30(s, 1H, isoxazole-H),7.42(d, 2H, J=13.2Hz),7.58-7.60(m, 2H),7.92-7.94(m, 2H), 8.68(s, 1H). J = 6.6 Hz, lH, NH), 5.83-5.85 (m, 2H, CH 2 ), 7.30 (s, 1H, isoxazole-H), 7.42 (d, 2H, J = 13.2 Hz), 7.58-7.60 (m) , 2H), 7.92-7.94 (m, 2H), 8.68 (s, 1H).
Q-41 3.35(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, 2CH2), 4.34(t, Q-41 3.35(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H, 2CH 2 ), 4.34(t,
J=6.6Hz,lH, NH), 5.86-5.87(m, 2H, CH2), 7.18(s, 1H, isoxazole-H),7.40(d, 2H, J=11.2Hz),7.58-7.61(m, lH),7.74-7.76(m, lH),7.85-7.86(m,lH), 8.69(s, 1H). J = 6.6 Hz, lH, NH), 5.86-5.87 (m, 2H, CH 2 ), 7.18 (s, 1H, isoxazole-H), 7.40 (d, 2H, J = 11.2 Hz), 7.58-7.61 (m) , lH), 7.74-7.76 (m, lH), 7.85-7.86 (m, lH), 8.69 (s, 1H).
Q-42 3.36(s, 6H, 2CH3), 3.72-3.76(m, 4H, 2CH2), 4.25-4.33(m, 4H, 2CH2), 4.34(t, Q-42 3.36(s, 6H, 2CH 3 ), 3.72-3.76(m, 4H, 2CH 2 ), 4.25-4.33(m, 4H, 2CH 2 ), 4.34(t,
J=6.6Hz,lH, NH), 5.83-5.85(m, 2H, CH2), 7.30(s, 1H, isoxazole-H),7.40(d, 2H, J=12.8Hz),7.72-7.74(m, 2H), 7.85-7.87(m, 2H), 8.68(s, 1H). 的制备 J = 6.6 Hz, lH, NH), 5.83-5.85 (m, 2H, CH 2 ), 7.30 (s, 1H, isoxazole-H), 7.40 (d, 2H, J = 12.8 Hz), 7.72 - 7.74 (m) , 2H), 7.85-7.87(m, 2H), 8.68(s, 1H). Preparation
( 1 ) 4-[(3-苯基-异噁唑 -5-基) -甲氧基 -]-喹唑啉盐酸盐  (1) 4-[(3-Phenyl-isoxazole-5-yl)-methoxy-]-quinazoline hydrochloride
将 0.5mmol 4-[(3-苯基-异噁唑 -5-基) -甲氧基-] -喹唑啉加入 20 mL ( V: V, 1 : 1 ) 5%的盐酸溶液和甲醇混合溶液中， 微热搅拌使其溶解后， 室温缓慢蒸发 结晶即得 4-[(3-苯基-异噁唑 -5-基) -甲氧基小喹唑啉盐酸盐， 白色固体， 收率： 68%。  Add 0.5 mmol of 4-[(3-phenyl-isoxazol-5-yl)-methoxy-]-quinazoline to 20 mL (V: V, 1:1) 5% hydrochloric acid solution and methanol After the solution is dissolved by stirring with a slight heat, the crystals are slowly evaporated at room temperature to obtain 4-[(3-phenyl-isoxazol-5-yl)-methoxyssalaquinazoline hydrochloride as a white solid. Rate: 68%.
(2 ) 4-[p-苯基-异噁唑 -5-基:) -甲氧基-] -喹唑啉乙酸盐的制备：  (2) Preparation of 4-[p-phenyl-isoxazole-5-yl:)-methoxy-]-quinazoline acetate:
将 0.5mmol 4-[(3-苯基-异噁唑 -5-基) -甲氧基小喹唑啉加入盛有 10 mL干燥 二氯甲垸的 50mL 单口圆底烧瓶中， 搅拌下加入 2mL冰乙酸， 30 °C~40°C搅 拌 l~2h,冷却后冷藏结晶， 过滤， 真空干燥后即得 4-[p-苯基-异噁唑 -5-基:) -甲 氧基小喹唑啉乙酸盐， 无色固体， 收率： 58%。 表 4-式 （I) 所示的化合物分别和有机酸或无机酸所成的盐  Add 0.5 mmol of 4-[(3-phenyl-isoxazol-5-yl)-methoxy small quinazoline to a 50 mL single-mouth round bottom flask containing 10 mL of dry dichloromethane, and add 2 mL with stirring. Glacial acetic acid, stirring at 30 °C ~ 40 °C for l~2h, cooling and crystallization, filtration, vacuum drying to obtain 4-[p-phenyl-isoxazol-5-yl:)-methoxy quinine Oxazoline acetate, colorless solid, yield: 58%. Table 4 - Salts of the compounds of formula (I) and organic or inorganic acids, respectively
Figure imgf000030_0001
甲酸 甲酸盐
Figure imgf000030_0001
Formic acid formate
乙酸 乙酸盐 草酸 草酸盐 柠檬酸 柠檬酸盐 富马酸 富马酸盐
Figure imgf000031_0001
Acetate acetate oxalate oxalate citrate citrate fumarate fumarate
Figure imgf000031_0001
马来酸 马来酸盐 苹果酸 苹果酸盐 乳酸 乳酸盐 酒石酸 酒石酸盐 对甲苯磺酸 对甲苯磺酸盐 三氟乙酸 三氟乙酸盐 氨基酸 氨基酸盐 实施例 5 生物活性试验  Maleic acid maleate malic acid malate lactic acid lactate tartrate tartrate p-toluenesulfonic acid p-toluenesulfonate trifluoroacetic acid trifluoroacetate amino acid amino acid salt Example 5 biological activity test
本发明式 （I) 所示的喹唑啉化合物或其药学上可接受的盐作为 EGFR酶 抑制剂， 采用酶联免疫吸附法测定所列举的化合物抑制 EGFR酶活性。  The quinazoline compound represented by the formula (I) or a pharmaceutically acceptable salt thereof is used as an EGFR enzyme inhibitor, and the exemplified compounds are assayed for inhibition of EGFR enzyme activity by an enzyme-linked immunosorbent assay.
具体的实验歩骤如下：  The specific experimental steps are as follows:
(1) 酶反应底物 Poly Glu,Tyr;i4:1用无钾离子的 PBS稀释成 2(^g/mL ， 包 被酶标板， 置于 37°C反应 12~16h后， 弃去孔中液体。 (1) Enzyme reaction substrate Poly Glu, Tyr; i 4:1 diluted with potassium-free PBS into 2 (^ g /mL, coated with the enzyme plate, placed at 37 ° C reaction for 12 ~ 16h, discarded Liquid in the well.
(2) 接着用 T-PBS洗板三次， 每次 lO min  (2) Then wash the plate three times with T-PBS, each time lO min
(3) 于 37°C烘箱中干燥酶标板  (3) Dry the ELISA plate in an oven at 37 ° C
(4) 包被好的酶标板孔内加入受试样品 （其中受试样品临用前先用 DMSO配制成 1 x10— 2M的储备液， 再用反应缓冲液稀释到所需的浓度后加至 实验孔内， 使其在 10( L反应体系中达到相应的终浓度。 (4) well microtiter plate wells were coated test sample was added (wherein immediately prior to the first test sample in DMSO stock solution formulated as a 1 x10- 2 M, then the reaction to the desired dilution buffer Add to the concentration Within the experimental wells, the corresponding final concentration was reached in 10 (L reaction system).
(5) 加入 ATP和受试酪氨酸激酶 (5) Add ATP and tested tyrosine kinase
加入用反应缓冲液稀释的 ATP溶液 (ATP终浓度为 5μΜ)及用反应缓冲液 稀释的受试酪氨酸激酶。反应的总体积为 10( L。同时设立阴性对照孔和无酶 对照孔。  ATP solution diluted with reaction buffer (5 μ at a final ATP concentration) and a test tyrosine kinase diluted in reaction buffer were added. The total volume of the reaction was 10 (L. Negative control wells and enzyme-free control wells were also established.
(6) 将反应体系置于湿盒内， 37 °C摇床避光反应 lh， 反应结束后 T-PBS 洗板 3次。 (6) The reaction system was placed in a wet box, shaken at 37 °C for 1 h, and washed with T-PBS 3 times after the reaction.
(7) 每孔中加入抗体 PY99 \00μ , 37 °C摇床反应 30min， T-PBS洗板 3 次。 (7) Add PY99 \00μ to each well, shake for 37 minutes at 37 °C, and wash the plate 3 times with T-PBS.
(8) 每孔中加入辣根过氧化物酶标记的羊抗鼠的 IgG \00μ^ 37 °C摇床反 应 30min， T-PBS洗板 3次。 (8) Horseradish peroxidase-labeled goat anti-mouse IgG was added to each well for 30 min., and the plate was washed 3 times with T-PBS.
(9) 每孔中加入 OPD显色液 10( L， 室温避光反应 l-10min。 (9) Add OPD coloring solution 10 (L, each room to avoid light reaction for l-10min.
(10) 每孔中加入 2M的 ¾S04溶液终止反应， 用可调波长式微孔板酶标 仪测 A49Q值。 采用如下的公式计算酶抑制率。 (10) Stop the reaction by adding 2M 3⁄4S0 4 solution to each well, and measure the A4 9Q value with a tunable wavelength microplate reader. The enzyme inhibition rate was calculated using the following formula.
化合物的 OD值 -无酶对照孔 OD值  OD value of the compound - no enzyme control well OD value
抑制率 （％) = X 00  Inhibition rate (%) = X 00
阴性对照 OD值 -无酶对照孔 OD值 采用如上的活性测试方法， 测得式 (I)所示的化合物或其盐对 EGFR酶的 抑制活性。  Negative control OD value - no enzyme control well OD value The inhibitory activity against the EGFR enzyme of the compound represented by the formula (I) or its salt was measured by the above activity test method.
表 5-部分化合物在 ΙΟΟμΜ浓度下抑制 EGFR酶的活性测试结果  Table 5 - Results of inhibition of EGFR enzyme activity by some compounds at ΙΟΟμΜ concentration
Figure imgf000032_0001
Figure imgf000033_0001
Figure imgf000032_0001
Figure imgf000033_0001

Claims

权 利 要 求 Rights request
1. 一种式①所示的喹唑啉化合物或其药学上可接受的盐  A quinazoline compound represented by Formula 1, or a pharmaceutically acceptable salt thereof
Figure imgf000034_0001
其中： 和 R2各自独立地选自氢， d~C6垸氧基， 卤代 d~C6垸氧基， C^ 垸氧基 d 垸氧基， C3-C8环垸氧基， 含有至少一个选自 N， 0， S杂 原子的 C3-C8杂环垸氧基；
Figure imgf000034_0001
Wherein: and R 2 are each independently selected from the group consisting of hydrogen, d-C 6 decyloxy, halo d-C 6 decyloxy, C 垸 methoxy d-methoxy, C 3 -C 8 cyclodecyloxy, a C 3 -C 8 heterocyclic alkoxy group containing at least one hetero atom selected from N, 0, S;
Z为 -Ν -， C(R5)2, S或 -0-， 其中 为氢或 d~C3垸基， R5相同或不同， 选自氢或 C^Cs垸基； Z is -Ν -, C(R 5 ) 2 , S or -0-, wherein is hydrogen or d~C 3 fluorenyl, R 5 is the same or different and is selected from hydrogen or C^Cs fluorenyl;
选自氢、 卤素， C广 C6垸基、 d~C6垸氧基或卤代 d~C6垸基； n为 0-5 的整数。 It is selected from the group consisting of hydrogen, halogen, C-C 6 fluorenyl, d-C 6 decyloxy or halogenated d-C 6 fluorenyl; n is an integer from 0 to 5.
2. 根据权利要求 1的喹唑啉化合物或其药学上可接受的盐， 其中  The quinazoline compound according to Claim 1 or a pharmaceutically acceptable salt thereof, wherein
和 为氢， C^Cs垸氧基， d~C6垸氧基 d~C6垸氧基， 含有至少一个 选自 N， 0， S杂原子的 C3-C8杂环垸氧基； And hydrogen, C^Cs methoxy, d~C 6 methoxy d~C 6 methoxy, containing at least one C 3 -C 8 heterocyclomethoxy group selected from N, 0, S heteroatoms;
Z为 -NH-, C¾或 -0-；  Z is -NH-, C3⁄4 or -0-;
选自氢、 氟、 氯、 溴、 甲基、 甲氧基或三氟甲基， n优选为 1-4， 更优 选 2-3。  It is selected from hydrogen, fluorine, chlorine, bromine, methyl, methoxy or trifluoromethyl, and n is preferably from 1 to 4, more preferably from 2 to 3.
3. 根据权利要求 1或 2的喹唑啉化合物或其药学上可接受的盐， 其中 Z为 -NH-或 -O-The quinazoline compound according to claim 1 or 2, or a pharmaceutically acceptable salt thereof, wherein Z is -NH- or -O-
R 禾口 R2为氢， C「C3烧氧基， C「C3烧氧基 C「C3烧氧基； R and R 2 are hydrogen, C "C 3 alkoxylate, C "C 3 alkoxy C "C 3 alkoxy;
R3优选在异噁唑环的邻位或对位， 更优选为 4-氟、 4-氯、 2-氯、 4-溴、 2,4- 二氯、 4-甲基、 4-甲氧基、 氢、 4-三氟甲基或 2,4-二甲氧基。 R 3 is preferably ortho or para to the isoxazole ring, more preferably 4-fluoro, 4-chloro, 2-chloro, 4-bromo, 2,4-dichloro, 4-methyl, 4-methoxy Base, hydrogen, 4-trifluoromethyl or 2,4-dimethoxy.
4.根据权利要求 1-3任一项的喹唑啉化合物或其药学上可接受的盐， 其中所述 化合物选自： The quinazoline compound or a pharmaceutically acceptable salt thereof according to any one of claims 1 to 3, wherein the compound is selected from the group consisting of:
4-[(3-苯基-异噁唑 -5-基) -甲氧基 -]-喹唑啉；  4-[(3-phenyl-isoxazole-5-yl)-methoxy-]-quinazoline;
4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氧基+喹唑啉；  4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methoxy+quinazoline;
4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: |-甲氧基+喹唑啉；  4-{[3-(2,4-dichlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-甲基苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-methylphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-甲氧基苯基) -异噁唑 -5-基: |-甲氧基+喹唑啉；  4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基： |-甲氧基+喹唑啉；  4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-[(3-苯基-异噁唑 -5-基) -甲氧基小喹唑啉；  6,7-dimethoxy-4-[(3-phenyl-isoxazole-5-yl)-methoxy small quinazoline;
6,7-二甲氧基 -4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氧基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二氯苯基 异噁唑 -5-基: |-甲氧基+喹唑啉； 6,7-dimethoxy-4-{[3-(2,4-dichlorophenyl isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲基苯基 异噁唑 -5-基： |-甲氧基+喹唑啉； 6,7-二甲氧基 -4-{[3-(4-甲氧基苯基 )-异噁唑 -5-基： I-甲氧基+喹唑啉； 6,7-二甲氧基 -4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氧基 - 喹唑啉； 6,7-二甲氧基 -4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基： |-甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基) ]-4-[(3-苯基-异噁唑 -5-基) -甲氧基小喹唑啉； 6,7-dimethoxy-4-{[3-(4-methylphenylisoxazol-5-yl: |-methoxy+quinazoline; 6,7-Dimethoxy-4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: I-methoxy+quinazoline; 6,7-dimethoxy 4-{[3-(4-Trifluoromethylphenyl)-isoxazol-5-yl: |-methoxy-quinazoline; 6,7-dimethoxy-4-{[ 3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methoxy+quinazoline; [6,7-bis(2-methoxyethoxy)]- 4-[(3-phenyl-isoxazol-5-yl)-methoxy small quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氧基+喹唑啉； [6,7-二 (2-甲氧乙氧基 )]-4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: |-甲氧基 +喹唑 啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-fluorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline; 6,7-bis(2-methoxyethoxy)]-4-{[3-(4-chlorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline; [6 ,7-bis(2-methoxyethoxy)]-4-{[3-(2-chlorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline; [6, 7-bis(2-methoxyethoxy)]-4-{[3-(4-bromophenyl)-isoxazol-5-yl]-methoxy+quinazoline; [6,7- Bis(2-methoxyethoxy)]-4-{[3-(2,4-dichlorophenyl)-isoxazol-5-yl: |-methoxy+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氧基- } -喹唑 啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methylphenyl)-isoxazol-5-yl]-methoxy- }-quinazoline ;
[6,7-二 (2-甲氧乙氧基) ]-4-{ [3-(4-甲氧基苯基) -异噁唑 -5-基] -甲氧基- } -喹唑 啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methoxyphenyl)-isoxazol-5-yl]-methoxy-}-quinazoline Porphyrin
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基] -甲氧基- } -喹 唑啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-trifluoromethylphenyl)-isoxazol-5-yl]-methoxy-}-quin Oxazoline
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2,4-二甲氧基苯基) -异噁唑 -5-基] -甲氧基+ 喹唑啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dimethoxyphenyl)-isoxazol-5-yl]-methoxy+ quin Oxazoline
4-[(3-苯基-异噁唑 -5-基) -甲氨基 -]-喹唑啉；  4-[(3-phenyl-isoxazole-5-yl)-methylamino-]-quinazoline;
4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氨基+喹唑啉； 4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: I-甲氨基+喹唑啉； 4-{[3-(4-bromophenyl)-isoxazol-5-yl]-methylamino+quinazoline; 4-{[3-(2,4-dichlorophenyl)-isoxazol-5-yl: I-methylamino+quinazoline;
4-{[3-(4-甲基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-methylphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-甲氧基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基： |-甲氨基+喹唑啉；  4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-[(3-苯基-异噁唑 -5-基) -甲氨基小喹唑啉；  6,7-dimethoxy-4-[(3-phenyl-isoxazole-5-yl)-methylaminosodium quinazoline;
6,7-二甲氧基 -4-{[3-(4-氟苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-fluorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氨基+喹唑啉；  6,7-dimethoxy-4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二氯苯基 异噁唑 -5-基: |-甲氧基+喹唑啉； 6,7-dimethoxy-4-{[3-(2,4-dichlorophenyl isoxazole-5-yl: |-methoxy+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉； 6,7-dimethoxy-4-{[3-(4-methylphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-甲氧基苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉； 6,7-dimethoxy-4-{[3-(4-methoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
6,7-二甲氧基 -4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基： |-甲氨基 - 喹唑啉；6,7-dimethoxy-4-{[3-(4-trifluoromethylphenyl)-isoxazole-5-yl: |-methylamino-quinazoline;
6,7-二甲氧基 -4-{[3-(2,4-二甲氧基苯基 )-异噁唑 -5-基： |-甲氨基+喹唑啉；6,7-dimethoxy-4-{[3-(2,4-dimethoxyphenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-[(3-苯基-异噁唑 -5-基) -甲氨基小喹唑啉； [6,7-bis(2-methoxyethoxy)]-4-[(3-phenyl-isoxazol-5-yl)-methylaminosauline;
[6,7-二（2-甲氧乙氧基) ]-4-{[3-(4-氟-苯基) -异噁唑 -5-基] -甲氨基- } -喹唑啉； [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-fluoro-phenyl)-isoxazole-5-yl]-methylamino- }-quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；[6,7-bis(2-methoxyethoxy)]-4-{[3-(4-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2-氯苯基) -异噁唑 -5-基： |-甲氨基+喹唑啉；[6,7-bis(2-methoxyethoxy)]-4-{[3-(2-chlorophenyl)-isoxazole-5-yl: |-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-溴苯基) -异噁唑 -5-基] -甲氨基+喹唑啉；[6,7-bis(2-methoxyethoxy)]-4-{[3-(4-bromophenyl)-isoxazole-5-yl]-methylamino+quinazoline;
[6,7-二 (2-甲氧乙氧基 )]-4-{[3-(2,4-二氯苯基) -异噁唑 -5-基: |-甲氨基 +喹唑 啉； [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dichlorophenyl)-isoxazol-5-yl: |-methylamino+quinazoline ;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-甲基苯基) -异噁唑 -5-基] -甲氨基- } -喹唑 啉； [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methylphenyl)-isoxazol-5-yl]-methylamino- }-quinazoline Porphyrin
[6,7-二 (2-甲氧乙氧基) ]-4-{ [3-(4-甲氧基苯基) -异噁唑 -5-基] -甲氨基- } -喹唑 啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-methoxyphenyl)-isoxazol-5-yl]-methylamino- }-quinazoline ;
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(4-三氟甲基苯基) -异噁唑 -5-基] -甲氨基- } -喹 唑啉；  [6,7-bis(2-methoxyethoxy)]-4-{[3-(4-trifluoromethylphenyl)-isoxazol-5-yl]-methylamino- }-quinazoline Porphyrin
[6,7-二 (2-甲氧乙氧基) ]-4-{[3-(2,4-二甲氧基苯基) -异噁唑 -5-基] -甲氨基+ 喹唑啉。  [6,7-bis(2-methoxyethoxy)]-4-{[3-(2,4-dimethoxyphenyl)-isoxazol-5-yl]-methylamino+ quinazole Porphyrin.
5.—种药物组合物， 其包括权利要求 1-4任一项的式 (I)所示的喹唑啉化合物， 或其药学上可接受的盐， 以及至少一种药学上可接受的、 惰性的、 无毒的赋 形剂或载体。  5. A pharmaceutical composition comprising the quinazoline compound of formula (I) according to any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable, An inert, non-toxic excipient or carrier.
6.用作药物的权利要求 1-4 的式 （I) 所示的喹唑啉化合物或其药学上可接受 的盐， 尤其是一种用于治疗对于抑制 EGFR过渡表达和 /或活性过高有效的肿 瘤的药物。  6. A quinazoline compound of the formula (1-4) according to Claims 1-4, or a pharmaceutically acceptable salt thereof, for use as a medicament, in particular for the treatment of inhibiting EGFR transient expression and/or hyperactivity An effective tumor drug.
7. 一种权利要求 1-4任一项的式 （I) 所示的喹唑啉化合物或其药学上可接受 的盐在制备用于抗肿瘤或癌症药物中的应用。  A use of a quinazoline compound of the formula (I) according to any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for use in antitumor or cancer.
8.根据权利要求 7所述的应用，其中所述的肿瘤或癌症是与 EGFR过渡表达和 /或活性过高的癌症。 更优选地， 所述肿瘤或癌症选自： 膀胱癌， 非小细胞肺 癌， 卵巢癌， 乳腺癌， 胃癌， 食道癌， 肺癌， 头颈癌， 结肠癌， 咽癌， 和胰 腺癌等， 更是非小细胞肺癌中的应用。  The use according to claim 7, wherein the tumor or cancer is a cancer that is transiently expressed and/or active with EGFR. More preferably, the tumor or cancer is selected from the group consisting of: bladder cancer, non-small cell lung cancer, ovarian cancer, breast cancer, gastric cancer, esophageal cancer, lung cancer, head and neck cancer, colon cancer, pharyngeal cancer, and pancreatic cancer, etc. Application in cell lung cancer.
9. 一种权利要求 1-4任一项的式（I)所示的喹唑啉化合物和 /或药学上可接受 的盐在制备抑制 EGFR的过渡表达和 /或活性过高的抑制剂。  A quinazoline compound and/or a pharmaceutically acceptable salt of the formula (I) according to any one of claims 1 to 4 for the preparation of an inhibitor which inhibits the transient expression and/or activity of EGFR.
10.—种权利要求 1-4任一项的式 (I)所示的喹唑啉化合物的制备方法， 其特征 以 6,7-二取代 4-氯-喹唑啉（式 II)和 3-取代苯基 -5-羟甲基-异噁唑（式 III) 或 3-取代苯基 -5-氨甲基-异噁唑（式 IV)为原料， 在干燥的有机溶剂和碱性缚 酸剂体系中反应制备； 10. A method for producing a quinazoline compound of the formula (I) according to any one of claims 1 to 4, characterized in that 6,7-disubstituted 4-chloro-quinazoline (formula II) and 3-substituted phenyl-5-hydroxymethyl-isoxazole (formula III) or 3-substituted phenyl-5-aminomethyl - Isoxazole (Formula IV) is a raw material, which is prepared by reacting in a dry organic solvent and a basic acid binding agent system;
Figure imgf000039_0001
Figure imgf000039_0001
如果需要， 可以将式 （II) 中的任何官能团予以保护;  Any functional group in formula (II) can be protected if necessary;
并且其后， 如果有必要 （以任何次序）：  And then, if necessary (in any order):
( 1 ) 除去任何保护剂， 和  (1) remove any protective agent, and
(2 ) 形成式 I化合物的药学上可接受的盐。  (2) Forming a pharmaceutically acceptable salt of a compound of formula I.
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