WO2013133882A3 - Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants - Google Patents

Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants Download PDF

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Publication number
WO2013133882A3
WO2013133882A3 PCT/US2012/069672 US2012069672W WO2013133882A3 WO 2013133882 A3 WO2013133882 A3 WO 2013133882A3 US 2012069672 W US2012069672 W US 2012069672W WO 2013133882 A3 WO2013133882 A3 WO 2013133882A3
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WO
WIPO (PCT)
Prior art keywords
isolation
double
allelic exchange
recombinant clostridium
exchange mutants
Prior art date
Application number
PCT/US2012/069672
Other languages
French (fr)
Other versions
WO2013133882A2 (en
Inventor
Eleftherios Papoutsakis
Mohab Ali AL-HINAI
Alan G. FAST
Original Assignee
University Of Delaware
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Delaware filed Critical University Of Delaware
Publication of WO2013133882A2 publication Critical patent/WO2013133882A2/en
Publication of WO2013133882A3 publication Critical patent/WO2013133882A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention provides recombinant Clostridium organisms having of double-crossover allelic exchange based on counter-selection and methods for isolation thereof.
PCT/US2012/069672 2011-12-16 2012-12-14 Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants WO2013133882A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161576588P 2011-12-16 2011-12-16
US61/576,588 2011-12-16
US201261700650P 2012-09-13 2012-09-13
US61/700,650 2012-09-13

Publications (2)

Publication Number Publication Date
WO2013133882A2 WO2013133882A2 (en) 2013-09-12
WO2013133882A3 true WO2013133882A3 (en) 2013-12-05

Family

ID=49117479

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/069672 WO2013133882A2 (en) 2011-12-16 2012-12-14 Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants

Country Status (1)

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WO (1) WO2013133882A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE051262T2 (en) 2014-01-28 2021-03-01 Lanzatech New Zealand Ltd Method of producing a recombinant microorganism
GB201406970D0 (en) 2014-04-17 2014-06-04 Green Biologics Ltd Targeted mutations
GB201406968D0 (en) 2014-04-17 2014-06-04 Green Biologics Ltd Deletion mutants
FR3025803B1 (en) * 2014-09-15 2018-02-02 IFP Energies Nouvelles EXPRESSION CASSETTE AND USES THEREOF
CN111088193B (en) * 2020-01-10 2022-09-09 南京工业大学 Method for improving electrotransformation frequency of methylotrophic butyric acid bacillus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010084349A1 (en) * 2009-01-22 2010-07-29 The University Of Nottingham Method of double crossover homologous recombination in clostridia
EP2316952A1 (en) * 2006-10-03 2011-05-04 Metabolic Explorer Process for chromosomal integration and DNA sequence replacement in clostridia

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2316952A1 (en) * 2006-10-03 2011-05-04 Metabolic Explorer Process for chromosomal integration and DNA sequence replacement in clostridia
WO2010084349A1 (en) * 2009-01-22 2010-07-29 The University Of Nottingham Method of double crossover homologous recombination in clostridia

Also Published As

Publication number Publication date
WO2013133882A2 (en) 2013-09-12

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