WO2013133481A1 - Photobioréacteur de type feuille de vinyle, et son procédé de fabrication - Google Patents

Photobioréacteur de type feuille de vinyle, et son procédé de fabrication Download PDF

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Publication number
WO2013133481A1
WO2013133481A1 PCT/KR2012/003839 KR2012003839W WO2013133481A1 WO 2013133481 A1 WO2013133481 A1 WO 2013133481A1 KR 2012003839 W KR2012003839 W KR 2012003839W WO 2013133481 A1 WO2013133481 A1 WO 2013133481A1
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Prior art keywords
sheet
reaction sheet
reaction
vinyl
carbon dioxide
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PCT/KR2012/003839
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English (en)
Korean (ko)
Inventor
오유관
박지연
김덕근
이진석
나정걸
김종남
한성옥
최은지
전상구
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한국에너지기술연구원
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Publication of WO2013133481A1 publication Critical patent/WO2013133481A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Definitions

  • the present invention relates to a vinyl sheet-type photobioreactor and a method of manufacturing the same, and more particularly, to arrange a plurality in a vertical form, a large-scale cultivation of photosynthetic microorganisms and a light-transmitting vinyl sheet-type photobioreactor and a method of manufacturing the same. It is about.
  • Photosynthetic microbes can grow using water, carbon dioxide, and sunlight, and can be cultured anywhere in the wilderness, on the coast, or in the sea, and thus do not compete with existing land crops in terms of land or space.
  • Photosynthetic microorganisms accumulate a large amount of lipids (up to 70%) in vivo depending on the culture conditions, and the production of oil (lipid) per unit area is more than 50-100 times higher than that of conventional edible crops such as soybeans. Very high.
  • Biodiesel which is produced from photosynthetic microorganisms such as microalgae, can reduce pollutants such as fine dust and sulfur compounds significantly compared to existing diesel fuels.
  • Photosynthetic microorganisms can be grown in large quantities and, unlike edible crops, can be harvested daily. In addition, photosynthetic microorganisms can directly absorb and grow high concentrations of carbon dioxide (15%) in by-product gases such as thermal power plants.
  • Photosynthetic microorganisms have also received great attention as potential production sources for high value-added medicines, pigments, cosmetics, protein and carbohydrate nutrients, and fine chemicals.
  • Carotene, Astaxanthin, Whole-cell dietary supplements, Whole-cell aquaculture feed, Polyunsaturated fatty acids, Heavy isotope labeled metabolites, Phycoerythrin (fluorescent label), Anticancer drugs Many products are sold worldwide, including pharmaceutical proteins.
  • the production technology of high value-added products using photosynthetic microorganisms consists of four processes: 1) cultivation of photosynthetic microorganisms, 2) harvesting, 3) extraction of useful substances, and 4) product conversion.
  • the process of culturing double photosynthetic microorganisms is very important in terms of economics of the whole process. For example, microalgal biofuel production technology costs 42%, 22%, 20% and 16% for microalgal culture, harvesting, oil extraction and biodiesel conversion for the entire process.
  • the form of a pond or a channel that circulates the medium through the outer ring is an example of a low installation cost and an operating cost, while it is difficult to cultivate a high concentration and contaminated by other microorganisms, thereby increasing the recovery cost of the photosynthetic product.
  • the form of a pond or a channel that circulates the medium through the outer ring is an example of a low installation cost and an operating cost, while it is difficult to cultivate a high concentration and contaminated by other microorganisms, thereby increasing the recovery cost of the photosynthetic product.
  • biofuels, pharmaceuticals, health food, feed using photosynthetic microorganisms such as high value-added production of the material is possible, especially in the biological CO 2 fixation is a high concentration of photosynthetic microbes in the process of mass culture techniques essentially required in the high culture efficiency along The demand for photobioreactors is increasing.
  • the bubble tower photobioreactor forms a chamber that can contain the microbial culture solution
  • a transparent outer column a light emitting body installed at the center of the outer column to irradiate light energy on the front surface of the culture medium, a transparent jacket separating heat from the light emitting medium and the heat exchanger, and installed on an outer surface of the transparent jacket
  • the present invention provides a vinyl sheet-type photobioreactor and a method of manufacturing the same, which are capable of cultivating a large number of photosynthetic microorganisms in a vertical arrangement and increasing light transmittance as a plurality of transmission units formed in the reaction sheet are arranged in a window form. will be.
  • the vinyl sheet type photobioreactor of the present invention for achieving the above object is formed of a culture space in which the photosynthetic microorganism and the culture medium are accommodated, and the front and rear surfaces of the vinyl material are joined to each other by thermal bonding to form a plurality of transmission parts. It may include a reaction sheet, a carbon dioxide supply unit for supplying carbon dioxide into the reaction sheet, and an entrance and exit unit for discharging oxygen generated by the photosynthesis of photosynthetic microorganisms from the carbon dioxide that was supplied into the reaction sheet.
  • PE polyethylene
  • LDPE low density polyethylene
  • LLDPE linear low density polyethylene
  • HDPE
  • the transmission part may be formed in a circular, elliptical, triangular or polygonal shape and arranged in a continuous or zigzag pattern in up, down, left, and right directions.
  • the transmission portion may be formed in a polygonal shape in which the accumulation portion is not formed at the top so that the photosynthetic microorganism does not become a accumulation, and may be formed in a continuous or zigzag arrangement.
  • the transmission part may have a transmission hole formed at the center thereof.
  • the carbon dioxide supply unit a supply pipe connected to the reaction sheet, the bubble pipe is connected to the supply pipe and inserted into the lower side of the reaction sheet and formed with a plurality of pores to supply carbon dioxide supplied from the supply pipe in the form of bubbles; And a supply pump installed at one side of the supply pipe to pump carbon dioxide into the reaction sheet.
  • the inlet and outlet part is formed on one surface of the reaction sheet to inject the culture medium and photosynthetic microorganisms, the inlet and outlet for discharging oxygen generated during the photosynthetic microorganism growth, the discharge pipe is connected to one side and the other side is extended to the inlet and outlet And a discharge pump installed at one side of the discharge pipe to pump oxygen to the outside.
  • a discharge port for discharging the photosynthetic microorganisms in the reaction sheet may be formed at the lower end of the reaction sheet.
  • a culture space in which the photosynthetic microorganisms and the culture medium are accommodated is formed, and the front and rear surfaces are joined by heat bonding to form a plurality of permeable parts.
  • Vinyl sheet-type photobioreactor manufacturing method of the present invention for achieving the above object, preparing a reaction sheet of a vinyl material formed with a culture space in which the photosynthetic microorganism and the culture medium is accommodated, and the front, rear of the reaction sheet Forming a plurality of permeation by bonding through a thermal bonding method, the reaction sheet installation step of supporting the upper and lower portions of the reaction sheet by a support and arranged in a vertical form, and for supplying carbon dioxide to the culture space of the reaction sheet And installing a carbon dioxide supply unit, and an inlet and outlet for discharging oxygen generated in the culture space of the reaction sheet.
  • the present invention can arrange a plurality of vinyl reaction sheets in a vertical form to enable mass cultivation of photosynthetic microorganisms and to reduce installation and management costs, and to provide a plurality of permeation units by thermal bonding to the reaction sheets. It is effective to increase productivity by increasing light transmittance by forming an array in the form of a window.
  • FIG. 1 is a perspective view showing the overall configuration of a vinyl sheet type photobioreactor according to an embodiment of the present invention.
  • FIG. 2 is an enlarged view of a portion 'A' of FIG. 1.
  • Figure 3 is a front view showing the overall configuration of a vinyl sheet type photobioreactor according to the present invention.
  • FIG. 4 is a view showing that the transmission portion of the reaction sheet according to the present invention is formed in a triangle.
  • 5 is a view showing that the transmission portion of the reaction sheet according to the present invention is formed in a polygon.
  • FIG. 6 is a view showing that the reaction sheet according to the present invention is installed in a plurality of parallel arrangement in the holding device.
  • FIG. 7 is an enlarged view of a portion 'B' of FIG. 6.
  • FIG. 8 is a view showing that a plurality of reaction sheets according to the present invention are arranged in parallel in a fixing device, and through holes are formed in the permeable portion of the reaction sheet.
  • FIG. 9 is a view showing that the permeate portion of the reaction sheet according to the present invention is formed in a circular shape and shifted from the permeate portions of the reaction sheets arranged in parallel.
  • FIG. 10 is a view showing that the permeate portion of the reaction sheet according to the present invention is formed in a triangle and shifted from the permeate portion of the reaction sheet arranged in parallel.
  • FIG. 11 is a view showing that the permeable portion of the reaction sheet according to the present invention is formed in a polygon and shifted from the permeation portions of the reaction sheets arranged in parallel.
  • FIG. 12 is a view showing that the transmission part of the reaction sheet according to the present invention is elongated in the shape of a polygon.
  • FIG. 13 is a flow chart showing a manufacturing method of a vinyl sheet-type photobioreactor according to the present invention.
  • FIG. 14 shows a photograph and a schematic diagram of a vinyl sheet type photobioreactor.
  • 15 shows a photograph and a schematic diagram of a multi-cylindrical plate type transparent film photobioreactor.
  • Figure 16 shows the cell concentration change of KR-1 according to the plate-shaped photobioreactor structure (vinyl sheet type vs. multi-cylindrical structure).
  • FIG. 17 shows changes in KR-1 production per reactor depending on the plate photobioreactor structure (vinyl sheet type vs. multi-cylindrical structure).
  • FIG. 18 shows a photo of a vinyl sheet type photobioreactor of various thermal bonding surfaces.
  • A The heat-adhesive surface is translucent,
  • B When the aluminum foil is attached,
  • C The heat-bonded part is cut out to form a transparent structure.
  • Reactor sequence is 1, 2, 3, 4 from front.
  • Figure 19 shows the difference in light dosage according to the heat bond surface shape and incubation time. Light dose is measured at the midpoint between reactor 1 and reactor 2 first (see FIG. 18).
  • Figure 21 shows the culture volume and the reactor thickness according to the thermal bond surface ratio.
  • Figure 23 shows the cell production and productivity according to the heat bond surface ratio.
  • FIG. 24 shows a dead zone photograph observed on an elliptic thermal bond surface.
  • FIG. 25 shows the cell concentration change of KR-1 according to the gas supply rate in the vinyl sheet type photobioreactor.
  • FIG. 26 shows a picture of a vinyl sheet type photobioreactor in which the thermally bonded portions form the i-type (A) and the ⁇ -type (B).
  • FIG. 27 shows the relative cell productivity and yield of KR-1 in a vinyl sheet type photobioreactor in which the thermally bonded portions are ⁇ (A) and ⁇ (B).
  • FIG. 1 to 3 illustrate a vinyl sheet type photobioreactor according to an exemplary embodiment of the present invention, which includes a reaction sheet 10, a carbon dioxide supply unit 20, an entrance and exit unit 30, and a transmission unit 12.
  • the reaction sheet 10 is made of a vinyl material and a culture space 11 is formed therein to accommodate photosynthetic microorganisms and culture medium, and a plurality of permeation parts 12 are formed on the front and rear surfaces by thermal bonding.
  • the reaction sheet 10 may be used without particular limitation as long as it is transparent to facilitate growth of photosynthetic organisms and has excellent light transmittance, and may include (a) polyethylene (PE), (b) low density polyethylene (LDPE), and (c) ) Linear low density polyethylene (LLDPE), (d) High density polyethylene (HDPE), (e) Oriented polypropylene (OPP), (f) Terephthalate and unstretched polypropylene mixed film (PET + CPP), (g) Unstretched poly Propylene (CPP), (h) Nylon (Nylon), (i) Biaxially stretched nylon (ON), (j) Unstretched nylon (CN), (k) Polyacetal (POM), (l) Polycarbonate (PC) (m) polyester, (n) polystyrene (PS), (o) polyester sulfone (PES), (p) polyvinyl chloride (PVC), (q) vinylidene chloride (PVDC), (r ) Eth
  • the reaction sheet 10 formed as described above has advantages such as light, transparent, and mechanical strength that are excellent in light transmittance, as compared to glass, acrylic, and the like, which are widely used as reaction vessel materials for photosynthetic bioreactors.
  • Photosynthetic microorganisms injected into the culture space 11 of the reaction sheet 10 are largely divided into microalgae, cyanobacteria, and photosynthetic bacteria.
  • microalgae is a generic term for single cell eukaryotic microorganisms having photosynthetic pigments and photosynthesis.
  • microalgae examples include Anacystis nidulans , Ankistrodesmus sp. , Biddulpha aurita , Botryococcus braunii , Catoserose ( Chaetoceros sp. ), Chlamydomonas applanata , Chlamydomonas reinhardtii , Chlorella sp.
  • Chlorella ellipsoidea Chlorella emersonii , Chlorella emersonii chlorella prototype Te koi death (chlorella protothecoides), chlorella Pierre noisy guru (chlorella pyrenoidosa), chlorella Thoreau Kearney Ana (chlorella sorokiniana), chlorella vulgaris (chlorella vulgaris), chlorella minu Tea Island (chlorella minutissima), chloro Caucus Margarito ralre (Chlorococcus littorale ), Cyclotella cryptica , Dunaliell a bardawil ), Dunaliella salina , Dunaliella tertiolecta , Dunaliella primolecta , Gymnodinum sp.
  • the bacterium is a generic term for bacteria that photosynthesize using chlorophyll in prokaryotes.
  • Such bacteria are anabena ( Anabaena sp. ), Carlottrix ( Calothrix sp. ), Camipon ( Chaemisiphon sp. ), Crocosidiopsis ( Chroococcidiopsis sp. ), Cyanothese ( Cyanothece sp. ), Cylindrospermum ( Cylindrospermum sp. ), Demo capella ( Dermocarpella sp. ), Picerella ( Fischerella sp. ), Gloeocop company ( Gloeocapsa sp. ), Myxosarsina ( Myxosarcina sp. ), Northstock ( Nostoc sp.
  • Oscillatoria Oscillatoria sp.
  • Formium Corium Phormidium corium
  • Flurocapsa Pleurocapsa sp.
  • Prochlorococcus Prochlorococcus sp.
  • Shudanabena Pseudanabaena sp.
  • Cinecoccus Synechococcus
  • Cinecosistis Synechocystis sp.
  • Toliforrix Tolypothrix sp.
  • Genococcus Xenococcus sp.
  • Etc. in the present invention, one or more of the above bacteria may be used.
  • the photosynthetic bacteria refers to bacteria that perform carbon assimilation by using light energy.
  • Rhodocista centenaria Rhodospira trueperi , Rhodospirillum fulvum , Rhodospirillum molischianum , Rhodospirillum molischianum , Rhodospirillum photometry ( Rhodospirillum photometricum ), Rhodospirillum rubrum , Rhodospirillum salexigens , Rhodospirillum salinarutn , Rhodospirillum salinarutn , Rhodospirillum sodomense ( Rhodospirillum sodomil Medio raised num (Rhodospirillum mediosalinum), also Pseudomonas (Rhodopseudomonas sp.), also Pseudomonas ash FIG pillar (Rhodopseudomonas acidophila),
  • the reaction sheet 10 may be partially immersed in water having a predetermined temperature to maintain a constant temperature of the photosynthetic microorganism and the culture medium in the culture space (11).
  • water may be stored in a container formed separately to immerse the reaction sheet 10 in water to soak a portion of the reaction sheet 10.
  • a part of the reaction sheet 10 may be immersed in seawater which is a natural environment. When using a natural environment, such as sea water is a method that can be used when the reaction sheet 10 is installed on a large scale.
  • the insertion hole 14 is formed in the horizontal longitudinal direction on the upper end of the reaction sheet 10, the support 40 is inserted.
  • the support 40 fixes the reaction sheet 10 to the fixing device 70 such that the reaction sheet 10 is spaced apart from the bottom.
  • the fixing device 70 is fixed to the floor.
  • the fixing sheet 15 is formed in the horizontal length direction at the lower side of the reaction sheet 10 so that the reaction sheet 10 is not deformed when the culture solution and the photosynthetic microorganism are injected into the reaction sheet 10 culture space 11.
  • the guide is inserted so that
  • a discharge port 60 is formed between the fixing hole 15 and the culture space 11 of the reaction sheet 10 to discharge the photosynthetic microbial culture solution, which has been grown in the culture space 11 of the reaction sheet 10, to the outside. have.
  • the discharge port 60 is provided with a valve 61 to open and close the discharge port 60 through the valve 61. Therefore, when the culture medium is injected into the reaction sheet 10, the valve 61 is blocked to close the discharge port 60. When discharging the completed photosynthetic microbial culture solution, the valve 61 is opened to open the discharge port 60. Open.
  • the transmission part 12 is a circular, elliptical, triangular or polygonal formed in a continuous arrangement up, down, left, and right in which no accumulation portion (not shown) to accumulate the circular or photosynthetic microorganisms, Light is transmitted through the transmission part 12 to supply light to the reaction sheet 10 installed at the rear side when the plurality of reaction sheets 10 are arranged, thereby improving the production efficiency of photosynthetic microorganisms.
  • each reaction sheet 10 arranged in a plurality of vertical arrangements may also be formed as a permeation unit 12 having a different arrangement, so that the reaction sheet 10 located at the rear side is provided. ) Can also provide light.
  • the most preferable arrangement of the transmission part 12 is a zigzag arrangement.
  • the transmission part 12 may form a transmission hole 13 by removing a predetermined portion of the inner side. Light is transmitted through the transmission hole 13 to irradiate the reaction sheet 10 with another reaction sheet 10 spaced apart from the predetermined interval.
  • the transmission hole 13 may have an area of 50 to 90% of the total area of the transmission part 12. This is because when the area of the transmission hole 13 is less than 50%, the amount of light transmitted is small and does not significantly affect the growth of photosynthetic microorganisms. When the area of the transmission hole 13 exceeds 90%, This is because the transmission part 12 may be damaged.
  • reaction sheet 10 of the transparent vinyl material By using the reaction sheet 10 of the transparent vinyl material it is easy to install in the field and can reduce the cost required for installation and management.
  • the permeation part 12 is formed in the ratio of the pressing surface is 5 to 35% of the total area of the reaction sheet. For this reason, if the ratio of the pressing surface is less than 5% as the photosynthetic microorganisms grow in the reaction sheet 10 over time, the reaction sheet 10 may be damaged due to weight, and the growth of the photosynthetic microorganisms is reduced. This is because problems can arise.
  • the ratio of the pressing surface of the permeation part 12 is greater than 35%, the ratio of the pressing part of the reaction sheet 10 is increased, so that the space for growing photosynthetic microorganisms may be reduced, thereby reducing the culture space. .
  • the permeation part 12 is formed by forming the respective forming area as small as possible, thereby increasing the number of permeation part 12 to increase the culture space 11 to prevent breakage of the reaction sheet 10 due to the weight. It is desirable to.
  • the carbon dioxide supply unit 20 is to supply carbon dioxide into the reaction sheet 10, the supply pipe 21 is connected to the reaction sheet 10, and is installed on one side of the supply pipe 21 carbon dioxide It consists of a supply pump 24 for pumping to supply to the culture space 11 of the reaction sheet 10, and the bubble pipe 22 is connected to the supply pipe 21 and inserted into the reaction sheet 10.
  • Carbon dioxide supplied through the carbon dioxide supply unit 20 is supplied in the reaction sheet 10 in the amount of 0.18 ⁇ 0.30 VVM.
  • the supply amount of carbon dioxide is less than 0.18VVM, there is a problem that the growth of photosynthetic microorganisms is slowed down because the photosynthetic microorganisms inside the reaction sheet 10 do not grow, and the supply amount of carbon dioxide is 0.30VVM
  • the overinjected carbon dioxide may slow down the growth of the photosynthetic microorganisms and may also cause photosynthesis by shear stress. Problems that inhibit the growth of microorganisms may occur.
  • Bubble tube 22 of the carbon dioxide supply unit 20 may be made of any one of stainless steel, polysulfone, polyethersulfone, polyvinylidene fluoride, Teflon, polyethylene, polypropylene and ceramics, silicon material.
  • the bubble tube 22 is a pore 23 is formed in the size of 0.1 ⁇ 30 um.
  • the pores 23 of the bubble tube 22 are formed to be less than 0.1 um, carbon dioxide supplied into the reaction sheet 10 may not be properly supplied so that the photosynthetic microorganisms in the reaction sheet 10 may not grow and the bubble size is too small. Shear stress is induced in photosynthetic microorganisms to inhibit growth.
  • the pores 23 of the bubble tube 22 are formed in excess of 30um, too large bubble sizes are formed so that carbon dioxide is not smoothly supplied into the reaction sheet 10 due to gas-liquid reaction restriction, and the culture solution in the reaction sheet 10 is also provided. In addition, it is not mixed smoothly, the inhibition of growth of photosynthetic microorganisms occurs due to the restriction of nutrients such as nitrogen source.
  • the bubble tube 22 may be inserted into a plurality of reaction sheets 10 to maintain the amount of carbon dioxide supplied according to the size of the reaction sheet (10).
  • the bubble tube 22 may be manufactured by adjusting the thickness of the bubble tube for each distance to maintain the amount of carbon dioxide supplied according to the size of the reaction sheet 10 and to prevent pressure loss.
  • the bubble tube 22 is formed to gradually decrease the diameter of the bubble tube 22 along the longitudinal direction in the direction opposite to the portion connected to the supply pipe 21.
  • the cross section of the bubble tube 22 is not limited to a circular shape can be produced in various forms such as oval, square, triangle, polygon.
  • the bubble pipe 22 may be provided with additional supply pipe 21, supply pump 24 on both sides of the bubble pipe (22).
  • the entrance and exit unit 30 discharges oxygen decomposed by photosynthetic action of photosynthetic microorganisms such as microalgae from carbon dioxide supplied into the reaction sheet 10, and injects the culture solution and the photosynthetic microorganism into the reaction sheet 10.
  • Discharge pump 33 for pumping to smoothly discharge the oxygen decomposed from the carbon dioxide may be installed in the discharge pipe (32).
  • a culture solution and photosynthetic microorganisms may be injected into the reaction sheet 10 into the inlet and outlet 31 of the inlet and outlet 30.
  • the discharge pipe 32 may be connected to the inlet and outlet 31 to discharge oxygen generated when the photosynthetic microorganisms injected into the reaction sheet 10 grow.
  • the reaction sheet 10 may be installed in parallel in a plurality of fixing devices 70 fixedly installed on the floor.
  • the transmissive portions 12 of the reaction sheets 10 arranged in parallel are formed in a zigzag arrangement on the side. The reason for this is that light is transmitted through the transmission part 12 of the reaction sheet 10 to illuminate the culture space 11 of the other reaction sheet 10 arranged in parallel.
  • the transmission part 12 may be formed to be elongated in the vertical direction of the reaction sheet 10.
  • the transmissive part 12 is formed in such a long shape, the light transmittance may be increased to increase the growth of photosynthetic microorganisms of the other reaction sheets 10 arranged in parallel.
  • the manufacturing method of the vinyl sheet-type photobioreactor according to the present invention includes preparing a reaction sheet 10 (S100), forming a transmission part 12 (S200), and a reaction sheet 10.
  • the reaction sheet (10) made of a vinyl material formed therein a culture space (11) in which the photosynthetic microorganisms and the culture medium are accommodated.
  • a circular or elliptical or triangular or pentagonal or stack ( Not formed) may be formed in a continuous polygon or zigzag arranged up, down, left and right, or arranged in a continuous or zigzag form in the form of a long polygon that does not form a stack (not shown).
  • the carbon dioxide supply unit 20 (S400), for supplying carbon dioxide to the culture space 11 of the reaction sheet 10, the carbon dioxide supply unit 20 is connected to the reaction sheet 10 A supply pipe 21 and a bubble pipe 22 which is connected to the supply pipe 21 to supply carbon dioxide into the reaction sheet 10 and installed on one side of the supply pipe 21 to supply carbon dioxide to the reaction sheet 10. It consists of a supply pump 24 for pumping to supply to the culture space (11) of.
  • the bubble tube 22 may be installed in a number depending on the size of the reaction sheet (10).
  • the step of installing the carbon dioxide supply unit 20 is to supply carbon dioxide to the bubble tube 22 through the supply pipe 21, the carbon dioxide is formed into air bubbles through the pores 23 of the bubble tube 22 inside the reaction sheet To be supplied.
  • the culture solution and the photosynthetic microorganisms are injected into the reaction sheet 10 into the culture space 11 of the reaction sheet 10, and oxygen generated when the photosynthetic microorganisms are grown.
  • the inlet and outlet unit 30 is an outlet 31 and the outlet 31 formed on one surface of the reaction sheet 10, the outlet 31 is connected to one side and the other side is discharge pipe 32 extending to the outside and Connected.
  • a discharge pump 33 for pumping to smoothly discharge oxygen generated due to photosynthesis from carbon dioxide to the outside may be installed at one side of the discharge pipe 32.
  • the step of installing the entrance and exit unit 30 is injecting the culture medium and photosynthetic microorganisms into the reaction sheet 10 through the entrance and exit 31 formed in the reaction sheet 10, discharge pipe to the entrance and exit 31 Through 32, oxygen generated during photosynthetic microbial growth is discharged to the outside.
  • the discharge pipe 32 connected to the inlet and outlet 31 may be connected to the discharge pump 33 to the discharge pipe in order to discharge the oxygen smoothly.
  • N8 medium for photosynthetic microbial culture using vinyl sheet type photobioreactor, Chlorella sp. KR-1 (KCTC0426BP) strain was used and N8 medium of nitrate concentration of 1 mM was used.
  • the composition of N8 medium was KNO 3 (0.1011 g / L), KH 2 PO 4 (0.7400 g / L), Na 2 HPO 4 (0.2598 g / L), MgSO 4 ⁇ 7H 2 O (0.0500 g / L), CaCl 2 (0.0132g / L), FeNaEDTA (0.0100g / L), ZnSO 4 ⁇ 7H 2 O (0.0032g / L), MnCl 2 ⁇ 4H 2 O (0.0130g / L), CuSO 4 (0.0117g / L) , Al 2 (SO 4 ) 3 .18H 2 O (0.0070 g / L).
  • the KR-1 strain was sequentially incubated in a constant temperature room equipped with a fluorescent lamp using a solid medium, a 250mL flask, a 1L cylindrical glass photobioreactor, and a 7L cylindrical glass photobioreactor, followed by inoculation at a level of 10% of the culture medium. .
  • the concentration of CO 2 in the feed gas was 10% (v / v), and the gas feed rates were 0.3 L / min and 0.75 L / min, respectively.
  • the temperature of the room temperature incubation chamber was 27-32 ° C., and the light intensity was 135-197 ⁇ mol / m 2 / s.
  • the experiment of the vinyl sheet type photobioreactor was carried out under natural conditions in an outdoor glass greenhouse built on the roof of a combustion flue gas R & D demonstration facility of Korea Institute of Energy Research, or in an indoor constant temperature culture chamber.
  • the CO 2 concentration of the supplied gas was 10% (v / v), and the feed rate and volume of air added to liquid volume per minute (VVM) were applied differently according to the culture volume and the reactor structure.
  • the window-structure transparent film sheet photobioreactor is heat-bonded with a semi-elliptic hot plate (area 72 cm2) using a low density polyethylene film (80 cm wide, 150 cm long, 0.08 mm thick) as a material. It was produced (Fig. 14).
  • the temperature of the hot plate was maintained at 160 ⁇ 170 °C during thermal bonding.
  • the semi-elliptic heat bonds were arranged in a zigzag to facilitate the distribution and light transmittance of the supplied gas in the photobioreactor.
  • the thermal bonding area was adjusted to 24 to 36% of the total area of the polyethylene film by changing the spacing and the number of semi-elliptic thermal bonding surfaces.
  • the spacing between the semi-elliptic thermal bond surfaces was 5 to 7 cm in the left and right and 3 to 9 cm in the top and bottom.
  • the gas was discharged and cultured through two entrances located above the vinyl sheet-type photobioreactor. Gas was supplied through the entrance of the lower left side of the reactor and the inner metal tube (Metal membrane, length 68cm, pore 0.1um) of the reactor, the photosynthetic microbial sample was taken through the entrance of the lower right. Unless otherwise specified, the vinyl sheet type photobioreactor was operated by installing four at regular intervals in an area of 70 cm to 80 cm.
  • Multi-column-structure transparent film sheet photo bioreactor was prepared based on the existing patent application (application number 10-2010-0005212) (Fig. 15).
  • the reactor was divided into seven evenly spaced polyethylene films through thermal bonding, and the total seven columns were manufactured in a connected shape.
  • Three silicon tubes were connected to the upper entrance of each cylinder unit for gas supply, gas outflow and sampling.
  • each cylinder was made in a V shape, and a spherical sintered air diffuser was connected to the gas supply silicon tube.
  • Dry weight was measured using the principle of the usual suspended solids measurement method. Photosynthetic microbial culture was filtered through a filter and dried to measure the weight difference before and after drying.
  • the correlation between OD 660nm and KR-1 dry weight (g / L) is as follows.
  • Example 2 Comparison of vinyl sheet type and multi-cylindrical flat type transparent film photobioreactor
  • Table 1 shows the fabrication and operation characteristics according to the structural differences of the vinyl sheet type and the multi-cylindrical plate type photobioreactor.
  • the cylindrical photobioreactor uses a conventional spherical sintered air diffuser
  • the vinyl sheet photobioreactor uses a tube-type stainless steel porous metal membrane.
  • Multi-cylindrical photobioreactors require a significant amount of silicon tubes because they consist of seven separate cylinders, and sampling, gas supply, and gas discharge through the top of each cylindrical structure.
  • the vinyl sheet-type photobioreactor consists of a single space, and an entrance for supplying gas or sampling is located at the bottom of the reactor, so that the length of the silicon tube can be reduced to about 1/8 of that of the cylindrical photobioreactor.
  • the number of entrances and silicone stoppers is about 1/2 of that of the multi-cylindrical vinyl sheet structure.
  • the flowmeter required for controlling the gas feed rate also has one vinyl sheet structure, which is considerably less than that of the cylindrical structure (7).
  • a multi-cylindrical photobioreactor In the case of photosynthetic microbial harvesting, a multi-cylindrical photobioreactor must be equipped with a pump because the sampling tube is located at the top of the reactor, but the vinyl sheet structured biobioreactor can be harvested by gravity without using a pump. .
  • the space-integrated vinyl sheet structure is manufactured and operated more than the space-type multi-cylinder structure. In terms of economics, we believe it will be advantageous.
  • the difference is expected to be even greater, especially considering the mass culture (hectare scale) of photosynthetic microorganisms.
  • FIG. 16 shows the growth curve of KR-1 according to the photobioreactor structure difference (window structure vs. multi-column-structure).
  • the cell growth rate was relatively higher than that of the multi-cylindrical structure.
  • the final cell concentration was 3.1 times higher than that of the cylindrical structure (absorbance 1.6).
  • the reactor thickness was thick as 8 cm, whereas in the case of the vinyl sheet structure, the reactor thickness was relatively thin (5 cm).
  • the thin-walled vinyl sheet-type photobioreactor has better light transmittance than the multi-cylindrical flat plate type photobioreactor, which shows relatively high cell growth rate and high final cell concentration.
  • the vinyl sheet type photobioreactor had 45% less culture volume due to the large thermal bonding area.
  • the final photosynthetic microbial biomass production was rather 5% higher (FIG. 17).
  • the vinyl sheet type photobioreactor developed in this study is considered to be more advantageous than the existing multi-cylindrical flat panel bioreactor.
  • the polyethylene film has a property that the bonding surface becomes opaque when thermally bonded.
  • the purpose of this study was to investigate the effect of thermal bonding surface morphology on the growth of photosynthetic microorganisms in vinyl sheet type photobioreactor.
  • the shape of the thermal bonding surface is (A) when the thermal bonding surface is translucent, (B) when the aluminum foil is attached so that light does not penetrate the thermal bonding surface, (C) the hole inside the thermal bonding surface as much as possible to transmit the light well. Investigation was carried out by dividing the case (Fig. 18).
  • the culture was carried out in an outdoor glass greenhouse.
  • the greenhouse temperature ranged from 20 to 41 ° C and the light intensity was from 0 to 875 ⁇ mol photons / m 2 ⁇ s.
  • Four photoreactors were operated at regular intervals in an area of 0.56 m 2 (70 cm * 80 cm).
  • the difference in light dosage according to the shape of the thermal bonding surface was measured at the midpoint between the first reactor and the second reactor in the morning, lunch and evening (FIG. 19).
  • the cell growth rate and final cell concentration were highest when the hole was thermally bonded (Transparent), followed by the case where the heat bonded surface was opaque (Opaque), and aluminum was attached to the heat bonded surface.
  • the ratio of thermal bonding area to the total film directly affects the culture volume and thickness of the reactor. That is, as the thermal bonding area increases, the thickness of the vinyl sheet-type photobioreactor decreases, and ultimately, the culture volume of the photobioreactor capable of holding a culture solution decreases.
  • the thickness of the reactor is reduced due to the reduced thermal bonding area, it is advantageous in terms of the culture volume of the reactor, but the length of light entering the reactor is long, so that the photosynthetic microorganisms in the reactor cannot receive enough light, which is disadvantageous in terms of photosynthetic efficiency of the photosynthetic microorganisms. do.
  • the thermal bonding area decreases, the volume increases may have a great influence on the durability of the vinyl reactor.
  • Figure 21 shows the correlation of the culture volume and the reactor thickness according to the thermal bonding ratio (24, 29, 36%).
  • the culture was carried out in an indoor constant temperature culture room.
  • the gas was supplied at a VVM of 0.18 in consideration of the culture volume, and the reactor was operated under the same experimental conditions except for the culture volume.
  • the thermal bonding rate of 29% and 36% showed similar cell growth rate and final cell concentration.
  • the heat conjugation rate of 24% showed lower cell growth rate and final cell concentration than the heat conjugation rate of 29% and 36%.
  • the effect of gas feed rate on cell growth of KR-1 in vinyl sheet type photobioreactor was investigated.
  • the culture was carried out in a constant temperature culture room equipped with a fluorescent lamp.
  • the heat bond rate and culture volume were 29% and 15L, respectively.
  • the gas feed rate was adjusted to a range of 2.0 to 5.0 L / min (VVM 0.13 to 0.33).
  • the effect of the thermal junction morphology (type Vs. ⁇ type) on the cell growth of KR-1 in the vinyl sheet type photobioreactor was examined (FIG. 26).
  • the culture was carried out in a constant temperature culture room equipped with a fluorescent lamp. Heat bond rate and culture volume were 29% and 15L, respectively.
  • the CO 2 concentration of the fed gas was 10% (v / v) and the feed rate was 3.75 L / min (0.25 VVM).
  • the present invention configured as described above is capable of cultivating large quantities of photosynthetic microorganisms and reducing installation and maintenance costs by arranging a plurality of vinyl reaction sheets in a vertical form. It is effective in increasing the productivity by increasing the light transmittance by forming an array in the form.

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Abstract

La présente invention concerne un photobioréacteur de type feuille de vinyle, une pluralité duquel peut être agencée selon une direction verticale de sorte à permettre la culture en masse de microorganismes photosynthétiques et à améliorer la transmission de la lumière. La présente invention concerne également un procédé de fabrication dudit photobioréacteur de type feuille de vinyle. Le photobioréacteur selon l'invention comprend une feuille de réaction constituée d'un matériau de vinyle, la feuille de réaction comportant, sur son intérieur, un espace de culture destiné à recevoir des microorganismes photosynthétiques et une solution de culture, la feuille de réaction présentant une surface avant et une surface arrière adhérant l'une à l'autre par adhésion thermique de sorte que de multiples parties de transmission de la lumière soient formées sur la feuille de réaction ; une unité d'alimentation en dioxyde de carbone permettant d'alimenter l'intérieur de la feuille de réaction en dioxyde de carbone ; et une unité d'entrée/sortie permettant de décharger l'oxygène généré à partir du dioxyde de carbone fourni à l'intérieur de la feuille de réaction par la photosynthèse des microorganismes photosynthétiques.
PCT/KR2012/003839 2012-03-06 2012-05-16 Photobioréacteur de type feuille de vinyle, et son procédé de fabrication WO2013133481A1 (fr)

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US9896652B2 (en) 2014-08-28 2018-02-20 Algenol Biofuels Switzerland GmbH Photobioreactor, system and method of use

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KR20160068541A (ko) 2014-12-05 2016-06-15 박나영 인공 광합성 기능을 갖는 가로등
KR102124119B1 (ko) * 2018-10-31 2020-06-17 전남대학교산학협력단 바이오 에너지 생산을 위한 조류 배양용 창호

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WO2008151376A1 (fr) * 2007-06-14 2008-12-18 Roger Stroud Appareil et procédé destinés à la culture de microorganismes photosynthétiques
KR20110008007A (ko) * 2008-01-18 2011-01-25 아베스톤 그리포드 리미티드 광생물 반응기
KR20110085428A (ko) * 2010-01-20 2011-07-27 성균관대학교산학협력단 투명 필름으로 이루어진 광합성 생물 반응기

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WO2008151376A1 (fr) * 2007-06-14 2008-12-18 Roger Stroud Appareil et procédé destinés à la culture de microorganismes photosynthétiques
KR20110008007A (ko) * 2008-01-18 2011-01-25 아베스톤 그리포드 리미티드 광생물 반응기
KR20110085428A (ko) * 2010-01-20 2011-07-27 성균관대학교산학협력단 투명 필름으로 이루어진 광합성 생물 반응기

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US9896652B2 (en) 2014-08-28 2018-02-20 Algenol Biofuels Switzerland GmbH Photobioreactor, system and method of use
WO2016056630A1 (fr) * 2014-10-08 2016-04-14 富士フイルム株式会社 Sac de culture cellulaire et procédé afférent
JP2016073246A (ja) * 2014-10-08 2016-05-12 富士フイルム株式会社 細胞培養バッグおよび細胞培養方法
EP3205710A4 (fr) * 2014-10-08 2017-10-25 Fujifilm Corporation Sac de culture cellulaire et procédé afférent

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