WO2013093762A1 - Compositions and methods for antibodies targeting factor p - Google Patents

Compositions and methods for antibodies targeting factor p Download PDF

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Publication number
WO2013093762A1
WO2013093762A1 PCT/IB2012/057394 IB2012057394W WO2013093762A1 WO 2013093762 A1 WO2013093762 A1 WO 2013093762A1 IB 2012057394 W IB2012057394 W IB 2012057394W WO 2013093762 A1 WO2013093762 A1 WO 2013093762A1
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WO
WIPO (PCT)
Prior art keywords
seq
nos
chain variable
variable region
antibody
Prior art date
Application number
PCT/IB2012/057394
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French (fr)
Inventor
Barbara Brannetti
Joy GHOSH
Ute Jaeger
Leslie Johnson
Yong-In Kim
Christian Carsten Silvester Kunz
Michael Roguska
Igor Splawski
Original Assignee
Novartis Ag
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Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47666430&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2013093762(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EA201491214A priority Critical patent/EA201491214A1/en
Priority to CN201280069461.3A priority patent/CN104114576B/en
Priority to AU2012356170A priority patent/AU2012356170B2/en
Priority to JP2014548290A priority patent/JP6231493B2/en
Priority to CA2859493A priority patent/CA2859493A1/en
Priority to EP12821311.3A priority patent/EP2794656B1/en
Priority to NZ626296A priority patent/NZ626296B2/en
Priority to ES12821311T priority patent/ES2728278T3/en
Priority to AP2014007761A priority patent/AP2014007761A0/en
Priority to KR1020147019752A priority patent/KR102159843B1/en
Priority to BR112014015111A priority patent/BR112014015111A2/en
Priority to SG11201403416TA priority patent/SG11201403416TA/en
Priority to MX2014007665A priority patent/MX2014007665A/en
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of WO2013093762A1 publication Critical patent/WO2013093762A1/en
Priority to TNP2014000258A priority patent/TN2014000258A1/en
Priority to ZA2014/04432A priority patent/ZA201404432B/en
Priority to MA37143A priority patent/MA35740B1/en
Priority to PH12014501410A priority patent/PH12014501410A1/en
Priority to IL233279A priority patent/IL233279B/en
Priority to US14/309,330 priority patent/US9988440B2/en
Priority to CR20140303A priority patent/CR20140303A/en
Priority to CU2014000073A priority patent/CU20140073A7/en
Priority to AU2016203134A priority patent/AU2016203134B2/en
Priority to US15/973,225 priority patent/US10865237B2/en
Priority to AU2018219993A priority patent/AU2018219993A1/en
Priority to AU2020203047A priority patent/AU2020203047A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A61K2039/507—Comprising a combination of two or more separate antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Definitions

  • Age related macular degeneration is a progressive disease and a leading cause of vision loss and blindness in Americans aged 65 and older. AMD primarily affects the macula; a part of the retina responsible for high visual acuity needed to read or drive. The majority of AMD patients suffer from an early stage of the disease which is characterized by the presence of extracellular retinal deposits called drusen. Drusen are extracellular retinal deposits of cell debris, inflammatory mediators, and extracellular matrix components. The late stages of AMD manifest as a dry or wet form, both are associated with vision loss. Dry AMD, also known as geographic atrophy, appears on ophthalmoscopic examination as clearly demarcated regions corresponding to local areas of retinal pigmented epithelium (RPE) loss.
  • RPE retinal pigmented epithelium
  • the innate human system is composed of the complement pathway.
  • the complement pathway serves to defend against pyogenic bacterial infection bridging innate and adaptive immunity; and disposing of products of immune complexes and inflammatory injury.
  • the complement is a system of more than 30 proteins involved in cascade reactions in plasma and cell surfaces.
  • the complement system and its complement components are involved in various immune processes.
  • complement C5b-9 complex also termed the terminal complex or the membrane attack complex (MAC) plays an important role in cell death by inducing membrane permeability damages.
  • Factor P may determine which cells will be lysed by attaching to a cell surface and functioning as a template on which convertases can form, leading to activation of the alternative complement pathway and lysis of the cell.
  • complement components C3 and C5 are principal constituents of drusen in patients with AMD. Mulling, R.F. et al. (2000) FASEB J 14, 835-46 Their presence as well as that of the membrane attack complex (MAC) C5b-9 and other acute phase reactant proteins in RPE cells overlying drusen has been speculated to be involved in the process that can trigger complement activation and formation of MAC. Johnson, L et al. (2001 ) Exp Eye Res 73, 887-896. Thus, there is growing evidence that complement components are more than mere mediators of innate immunity.
  • Nutritional intervention has been prescribed to inhibit progression of dry AMD to wet AMD. At present the only FDA approved treatments for wet AMD include
  • PDT photodynamic therapy
  • an anti-VEGF aptamer such as pegaptanib
  • anti-VEGF antibodies ranibizumab
  • the present invention relates to an isolated antibody, or antigen binding fragment thereof, that binds to human or cynomolgus Factor P, wherein said antibody binds to the TSR5 domain (SEQ ID NO: 406).
  • the antibodies, or antigen binding fragments described herein bind to a region of the TSR5 domain comprising the sequence of SEQ ID NO: 407, more specifically said antibodies also bind a region of the Factor P TSR5 domain comprising the amino acid sequence KSISC (SEQ ID NO: 408).
  • the isolated antibodies, or antigen binding fragments thereof bind to a Factor P epitope comprising the amino acid sequence of SEQ ID NO: 407.
  • the isolated antibodies, or antigen binding fragments thereof bind to a Factor P epitope comprising the amino acid sequence of SEQ ID NO: 408.
  • the isolated antibodies, or antigen binding fragments, described herein bind Factor P, with a KD of less than or equal to 1 .2 nM.
  • the isolated antibodies or antigen binding fragments described herein may bind to human or cynomolgus Factor P with a KD of less than or equal to 1.1 nM, less than or equal to 1 nM, less than or equal to 600pM, less than or equal to 500 pM, less than or equal to 400 pM, less than or equal to 300 pM, less than or equal to 200 pM, less than or equal to 100 pM, less than or equal to 75 pM, less than or equal to 50 pM, less than or equal to 40pM, less than or equal to 30 pM, less than or equal to 20 pM, or less than or equal to 10pM.
  • binding affinity of isolated antibodies and antigen binding fragments described herein can be determined by solution equilibrium titration (SET). Methods for SET are known in the art and are described in further detail below. Alternatively, binding affinity of the isolated antibodies, or fragments, described herein can be determined by Biacore assay. Methods for Biacore kinetic assays are know in the art and are described in further detail below.
  • an isolated antibody or antigen binding fragment thereof can inhibit the alternative complement pathway as measure by an in vitro hemolytic assay with an IC50 of less than or equal to 25 nm, less than or equal to 20 nM, less than or equal to 16nM, less than or equal to 15nM, less than or equal to 14nM, less than or equal to 13nM, less than or equal to 12nM, less than or equal to 1 1 nM, less than or equal to 10nM, less than or equal to 9nM, less than or equal to 8nM, less than or equal to 7nM. More specifically, an isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway in human as measure by an in vitro hemolytic assay with an IC50 of less than or equal to 16 nm, or less than or equal to 9 nm.
  • An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway as measure by an in vitro C3b deposition assay with an IC50 of less than or equal to 10 nm, less than or equal to 7nM, less than or equal to 6 nM, less than or equal to 5nM, less than or equal to 4 nM, less than or equal to 3 nM, less than or equal to 2 nM, less than or equal to 1 nM, less than or equal to 15nM, less than or equal to 1 nM, less than or equal to 0.5 nM, or less than or equal to 0.1 nM.
  • an isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway in human as measure by an in vitro C3b deposition assay with an IC50 of less than or equal to 3 nm, or less than or equal to 2 nM.
  • An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway with an IC50 of less than or equal to 25 nm, less than or equal to 20 nM, less than or equal to 15 nM, less than or equal to 10 nM, less than or equal to 9 nM, less than or equal to 8 nM, less than or equal to 7 nM, or less than or equal to 6 nM, as measure by deposition of the complement membrane attack complex. More specifically, an isolated antibody or fragment thereof as described herein can inhibit the alternative complement pathway in human with an IC50 of less than or equal to 25 nm, or less than or equal to 7.5 nM, as measure by deposition of the complement membrane attack complex.
  • An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C3a.
  • An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of iC3b.
  • An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C5a.
  • An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C5b.
  • An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway by destabilizing and/or blocking the activity of C3 and/or C5 convertase, as measured by a decrease in production of C3a, C3b, iC3b, C5a, and/or C5b.
  • An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the generation of C5a with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM.
  • the isolated antibodies, or antigen binding fragment thereof may also block Factor P binding to C3b and/or prevent Factor P binding to the cell surface or to DNA or oligonucleotides.
  • Another aspect of the invention includes an isolated antibody, or antigen binding fragment thereof, that specifically binds to human, cynomolgus, rat and/or rabbit Factor P.
  • the isolated antibody, or antigen binding fragment competes for binding with an antibody, or antigen binding fragment, described in Table 1.
  • the isolated antibodies, or antigen binding fragments thereof, as described herein can be a monoclonal antibodies, a human or humanized antibodies, a chimeric antibodies, single chain antibodies, Fab fragments, Fv fragments, F(ab')2 fragments, or ScFv fragments, and/or IgG isotypes.
  • the isolated antibodies, or antigen binding fragments thereof, as described herein can also include a framework in which an amino acid has been substituted into the antibody framework from the respective human VH or VL germline sequences.
  • Another aspect of the invention includes an isolated antibody or antigen binding fragment thereof having the heavy and light chain sequences of Fabs described in Table 1 .
  • the isolated antibody or antigen binding fragment thereof can have the heavy and light chain sequences of Fab NVS962, NVS963, NVS964, NVS965, NVS966, NVS967, NVS962-G, NVS962-S, NVS962-T, NVS962-Q, NVS962-S31A, NVS965-Q, NVS965-S, NVS965-T, NVS804, NVS805, NVS806, NVS807, or NVS808.
  • a further aspect of the invention includes an isolated antibody or antigen binding fragment thereof having the heavy and light chain variable domain sequences of Fabs described in Table 1 .
  • the isolated antibody or antigen binding fragment there of can have the heavy and light chain variable domain sequence of Fab NVS962 , NVS963, NVS964, NVS965, NVS966, NVS967, NVS962-G, NVS962-S, NVS962-T, NVS962-Q, NVS962-S31A, NVS965-Q, NVS965-S, NVS965-T, NVS804, NVS805, NVS806, NVS807, or NVS808.
  • the invention also relates to an isolated antibody or antigen binding fragment thereof that includes a heavy chain CDR1 selected from the group consisting of SEQ ID NOs 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, and 267; a heavy chain CDR2 selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268; and a heavy chain CDR3 selected from the group consisting of SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, and 269, wherein the isolated antibody or antigen binding fragment thereof binds to human Factor
  • the isolated antibody or antigen binding fragment thereof further includes a light chain CDR1 selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; a light chain CDR2 selected from the group consisting of SEQ ID NOs 5, 19, 33, 47, 61 , 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271; and a light chain CDR3 selected from the group consisting of SEQ ID NOs 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272.
  • a light chain CDR1 selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74
  • the invention also relates to an isolated antibody or antigen binding fragment thereof that includes a light chain CDR1 selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; a light chain CDR2 selected from the group consisting of SEQ ID NOs 5, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271 ; and a light chain CDR3 selected from the group consisting of SEQ ID NOs 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202,216, 230, 244, 258, and 272, wherein the isolated antibody or antigen binding fragment thereof binds to human Factor P.
  • the invention also relates to an isolated antibody or antigen binding fragment thereof that binds Factor P having HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs: 1, 2, 3, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 4, 5, 6; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 15, 16, 17, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 18, 19, 20; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 29, 30, 31, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 32, 33, 34; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR
  • the isolated antibody or antigen binding fragment thereof includes a heavy chain variable domain sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273.
  • the isolated antibody or antigen binding fragment further comprises a light chain variable domain seqeunce wherein the heavy chain variable domain and light chain variable domain combine to form and antigen binding site for Factor P.
  • the isolated antibody or antigen binding fragment further includes a light chain variable domain sequence selected from SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274 wherein said isolated antibody or antigen binding fragment thereof binds Factor P.
  • the invention also relates to an isolated antibody or antigen binding fragment thereof that includes a light chain variable domain sequence selected from the group consisting of SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, wherein said isolated antibody or antigen binding fragment thereof binds to human Factor P.
  • the isolated antibody or antigen binding fragment further comprises a heavy chain variable domain sequence wherein the light chain variable domain and heavy chain variable domain combine to form and antigen binding site for Factor P.
  • the isolated antibody or antigen binding fragment thereof that binds Factor P may have heavy and light chain variable domains comprising the sequences of SEQ ID NOs: 7 and 8; 21 and 22; 35 and 36; 49 and 50; 63 and 64; 77 and 78; 91 and 92; 104 and 105; 1 18 and 1 19; 132 and 133; 146 and 147; 160 and 161 ; 174 and 175; 188 and 189; 202 and 203; 216 and 217; 230 and 231 ; 244 and 245; 258 and 259; or 272 and 273, respectively.
  • the invention further relates to an isolated antibody or antigen binding fragment thereof, that includes a heavy chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273, wherein said antibody binds to Factor P.
  • the isolated antibody or antigen binding fragment thereof also includes a light chain variable domain having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274.
  • the isolated antibody or antigen binding fragment thereof may include a light chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, wherein said antibody binds Factor P.
  • the isolated antibody, or antigen binding fragment thereof, that binds to Factor P may have a heavy chain comprising the sequence of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275.
  • the isolated antibody also includes a light chain that can combine with the heavy chain to form an antigen binding site to human Factor P.
  • the isolated antibody or antigen binding fragment thereof includes a light chain having a sequence comprising SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276.
  • the invention still further relates to an isolated antibody or antigen binding fragment thereof that includes a heavy chain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 9, 23, 37, 51 , 65, 79, 93,
  • the isolated antibody or antigen binding fragment thereof also includes a light chain having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 10, 24, 38, 52, 66, 80, 94,
  • the invention still further relates to an isolated antibody or antigen binding fragment thereof that includes a light chain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 and 275, wherein said antibody binds Factor P.
  • the invention also relates to compositions comprising the isolated antibody, or antigen binding fragment thereof, described herein. As well as, antibody compositions in combination with a pharmaceutically acceptable carrier. Specifically, the invention further includes pharmaceutical compositions comprising an antibody or antigen binding fragment thereof of Table 1 , such as, for example antibody NVS962, NVS963, NVS964, NVS965, NVS966, NVS967, NVS962-G, NVS962-S, NVS962-T, NVS962-Q, NVS962- S31A, NVS965-Q, NVS965-S, NVS965-T, NVS804, NVS805, NVS806, NVS807, or NVS808.
  • the invention also realtes to pharmaceutical compositions comprising a combination of two or more of the isolated antibodies or antigen binding fragments thereof of Table 1.
  • the invention also relates to an isolated nucleic acid comprising a sequence encoding a polypeptide that includes a heavy chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273.
  • the invention also relates to an isolated nucleic acid comprising a sequence encoding a polypeptide that includes a light chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274.
  • the invention also relates to a vector that includes one or more of the nucleic acid molecules described herein.
  • the invention also relates to an isolated host cell that includes a recombinant DNA sequence encoding a heavy chain of the antibody described above, and a second recombinant DNA sequence encoding a light chain of the antibody described above, wherein said DNA sequences are operably linked to a promoter and are capable of being expressed in the host cell.
  • the antibody can be a human monoclonal antibody.
  • the host cell is a non-human mammalian cell.
  • the invention also relates to a method of inhibiting the complement mediated cell death wherein the method includes the step of contacting a cell with an effective amount of a composition comprising the isolated antibody or antigen binding fragments thereof described herein. It is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.
  • the invention still further relates to a method of inhibiting the alternative complement pathway in a cell wherein the method includes the step of contacting the cell with an effective amount of a composition comprising the isolated antibody or antigen binding fragments thereof described herein.
  • the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.
  • the invention also relates to a method of inhibiting the formation of membrane attack complex in a cell wherein the method includes the step of contacting the cell with an effective amount of a composition comprising the isolated antibody or antigen binding fragments thereof described herein. It is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.
  • any of the foregoing isolated antibodies or antigen binding fragments thereof may be a monoclonal antibody or antigen binding fragment thereof.
  • the invention provides a a first antibody, or antigen binding fragment thereof, that binds Factor P, and a second antibody, or antigen binding fragment thereof, that binds C5, wherein said combination inhibits the alternative complement pathway.
  • the first and second antibodies can be in combination as a composition.
  • Ocular inflammation can be determined by measuring neutrophil accumulation and/or macrophage recruitment in the retina.
  • such a combination can be used to inhibit neutrophil accumulation in the retina, or macrophage recruitment in the retina.
  • the antibody in such a combination that binds Factor P binds a region of Factor P comprising SEQ ID NO: 408.
  • such an antibody binds a region of Factor P comprising SEQ ID NO: 407.
  • the combination of antibodies or binding fragments thereof that bind Factor P and C5 include a first antibody or antigen binding fragment selected from Table 1 and a second antibody or antigen-binding fragment selected from Table 2.
  • the first antibody, or antigen binding fragment thereof binds the same epitope as is an antibody described in Table 1 and the second antibody, or antigen binding fragment thereof, binds the same epitope as is an antibody described in Table 2.
  • the invention provides a first antibody, or antigen binding fragment thereof that comprises a heavy chain CDR1 , 2, 3, and a light chain CDR1 , 2, 3, selected from the group consisting of a) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 , 2, and 3, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6,
  • a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 15, 16, and 17, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 18, 19, and 20, respectively;
  • the invention relates to a first and second antibody or antigen bidning fragement thereof (which may be in combination as a composition) where the first antibody or antigen binding fragment thereof includes heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 7 and 8; SEQ ID NOs: 21 and 22; SEQ ID NOs: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID NOs: 105 and 106; SEQ ID NOs: 1 19 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID NOs: 161 and 162; SEQ ID NOs: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID NOs: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID NOs: 231 and
  • the invention relates to a first and second antibody or antigen bidning fragement thereof (which may be in combination as a composition) where the first antibody or antigen binding fragment thereof includes heavy and light chain variable regions having amino acid sequences selected from SEQ ID NOs: 7 and 8; SEQ ID NOs: 21 and 22; SEQ ID NOs: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID NOs: 105 and 106; SEQ ID NOs: 1 19 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID NOs: 161 and 162; SEQ ID NOs: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID NOs: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID NOs: 231 and 232; SEQ ID NO
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereo includes a heavy chain variable region comprising SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259, or 273 and further includes a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a heavy chain variable region comprising SEQ ID NO: 416, 432, 448, 460 or 476 and further includes a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to C5.
  • the first antibody, or antigen binding fragment thereo includes a heavy chain variable region comprising SEQ ID NO: 7, 21
  • the first antibody or antigen binding fragment thereof includes the light chain variable region sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274, and the second antibody or antigen binding fragment thereof includes the light chain variable region sequence of SEQ ID NO: 417, 433, 449, 461 or 477.
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody or antigen bidning fragment thereof incldues a light chain variable domain comprising SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274 and further includes a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a light chain variable region comprises a light chain variable domain includes SEQ ID NO: 417, 433, 449, 461 or 477 and further comprises a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to C5.
  • the first antibody or antigen bidning fragment thereof incldues a light chain variable domain comprising
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further includes a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to C5.
  • the first antibody, or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163,
  • the first antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276, and wherein the second antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 419, 435, 451 , 463, or 479.
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereof includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and further includes a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 419, 435, 451 , 463, or 479 and further includes a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to C5.
  • the first antibody, or antigen binding fragment thereof includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178,
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further includes a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second antibody or antigen binding fragement thereof includes a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain with an amino acid sequence having at least
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain with an amino acid sequence seclected from SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further includes a light chain with an amino acid sequence selected from SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second antibody or antigen binding fragement thereof includes a heavy chain with an amino acid sequence selected from SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain with an amino acid sequence selected from SEQ ID NO: 419, 435, 4
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity, respectively, to SEQ ID NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52, 65 and 66, 79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity, respectively, to SEQ ID NOs: 418 and 419, 434 and 435; 450 and 451 ; 462 and 463; or 478 and 479.
  • the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence, respectively, selected from SEQ ID NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52, 65 and 66, 79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence, respectively, selected from SEQ ID NOs: 418 and 419, 434 and 435; 450 and 451 ; 462 and 463; or 478 and 479.
  • the invention further relates to an isolated nucleic acid molecule comprising a nucleotide sequence encoding the first and/or second antibody or antigen binding fragment thereof as described herein.
  • a nucleic acid sequence can be included in a vector, which may, in turn be included in a host cell which, in one aspect, is capable of expressing such nucleic acid sequence.
  • the invention further relates to a method of treating age related macular degeneration in a subject comprising administering to said subject, an effective amount of a first and second antibody or antigen binding fragment thereof, either singly, or in combination as a composition.
  • the subject may be a human.
  • the invention further releates to a method of inhibiting the alternative
  • complement pathway in a subject comprising administering to said subject an effective amount of a first and second antibody or antigen binding fragment thereof, either singly, or in combination as a composition.
  • the subject may be a human.
  • antibody as used herein means a whole antibodies and any antigen binding fragment (/ ' . e., "antigen-binding portion") or single chains thereof.
  • a whole antibody is a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds.
  • Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region.
  • the heavy chain constant region is comprised of three domains, CH1 , CH2 and CH3.
  • Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region.
  • the light chain constant region is comprised of one domain, CL.
  • VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR).
  • CDR complementarity determining regions
  • FR framework regions
  • Each VH and VL is composed of three CDRs and four FRs arranged from amino-terminus to carboxy- terminus in the following order: FR1 , CDR1 , FR2, CDR2, FR3, CDR3, FR4.
  • the variable regions of the heavy and light chains contain a binding domain that interacts with an antigen.
  • the constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system.
  • antigen binding portion or "antigen binding fragment” of an antibody, as used herein, refers to one or more fragments of an intact antibody that retain the ability to specifically bind to a given antigen (e.g., Factor P).
  • Antigen binding functions of an antibody can be performed by fragments of an intact antibody.
  • binding fragments encompassed within the term antigen binding portion or antigen binding fragment of an antibody include a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; a F(ab) 2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; an Fd fragment consisting of the VH and CH1 domains; an Fv fragment consisting of the VL and VH domains of a single arm of an antibody; a single domain antibody (dAb) fragment (Ward et al., 1989 Nature 341 :544-546), which consists of a VH domain or a VL domain; and an isolated complementarity determining region (CDR).
  • Fab fragment a monovalent fragment consisting of the VL, VH, CL and CH1 domains
  • F(ab) 2 fragment a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region
  • an Fd fragment consisting of the
  • the two domains of the Fv fragment, VL and VH are coded for by separate genes, they can be joined, using recombinant methods, by an artificial peptide linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see, e.g., Bird et al., 1988 Science 242:423-426; and Huston et al., 1988 Proc. Natl. Acad. Sci. 85:5879-5883).
  • Such single chain antibodies include one or more antigen binding portions or fragments of an antibody. These antibody fragments are obtained using conventional techniques known to those of skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.
  • Antigen binding fragments can also be incorporated into single domain antibodies, maxibodies, minibodies, intrabodies, diabodies, triabodies, tetrabodies, v- NAR and bis-scFv (see, e.g., Hollinger and Hudson, 2005, Nature Biotechnology, 23, 9, 1 126-1 136).
  • Antigen binding portions of antibodies can be grafted into scaffolds based on polypeptides such as Fibronectin type III (Fn3) (see U.S. Pat. No. 6,703,199, which describes fibronectin polypeptide monobodies).
  • Fn3 Fibronectin type III
  • Antigen binding fragments can be incorporated into single chain molecules comprising a pair of tandem Fv segments (VH-CH1 -VH-CH1 ) which, together with complementary light chain polypeptides, form a pair of antigen binding regions (Zapata et a/., 1995 Protein Eng. 8(10):1057-1062; and U.S. Pat. No. 5,641 ,870).
  • the term “affinity” refers to the strength of interaction between antibody and antigen at single antigenic sites. Within each antigenic site, the variable region of the antibody “arm” interacts through weak non-covalent forces with antigen at numerous sites; the more interactions, the stronger the affinity.
  • the term “high affinity” for an IgG antibody or fragment thereof refers to an antibody having a KD of 10 "8 M or less, 10 "9 M or less, or 10 "10 M, or 10 "11 M or less, or 10 "12 M or less, or 10 "13 M or less for a target antigen.
  • high affinity binding can vary for other antibody isotypes.
  • high affinity binding for an IgM isotype refers to an antibody having a KD of 10 "7 M or less, or 10 "8 M or less.
  • amino acid refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids.
  • Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, ⁇ -carboxyglutamate, and O-phosphoserine.
  • Amino acid analogs refer to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an alpha carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid.
  • Amino acid mimetics refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally occurring amino acid.
  • binding specificity refers to the ability of an individual antibody combining site to react with only one antigenic determinant.
  • an antibody e.g., a Factor P- binding antibody
  • an antibody refers to a binding reaction that is determinative of the presence of a cognate antigen (e.g., a human Factor P or cynomolgus Factor P) in a heterogeneous population of proteins and other biologies.
  • a cognate antigen e.g., a human Factor P or cynomolgus Factor P
  • an antibody recognizing an antigen and “an antibody specific for an antigen” are used interchangeably herein with the term “an antibody which binds specifically to an antigen”.
  • condition or disorders associated with macular degeneration refers to any of a number of conditions in which the retinal macula degenerates or becomes dysfunctional, e.g., as a consequence of decreased growth of cells of the macula, increased death or rearrangement of the cells of the macula (e.g., RPE cells), loss of normal biological function, or a combination of these events.
  • Macular degeneration results in the loss of integrity of the histoarchitecture of the cells and/or extracellular matrix of the normal macula and/or the loss of function of the cells of the macula.
  • macular degeneration-related disorder examples include AMD, North Carolina macular dystrophy, Sorsby's fundus dystrophy, Stargardt's disease, pattern dystrophy, Best disease, dominant drusen, and malattia leventinese (radial drusen).
  • AMD North Carolina macular dystrophy
  • Sorsby's fundus dystrophy Stargardt's disease
  • pattern dystrophy Best disease
  • dominant drusen radial drusen
  • malattia leventinese radial drusen
  • macular degeneration-related disorder also broadly includes any condition which alters or damages the integrity or function of the macula (e.g., damage to the RPE or Bruch's membrane).
  • the term encompasses retinal detachment, chorioretinal degenerations, retinal degenerations, photoreceptor degenerations, RPE degenerations, mucopolysaccharidoses, rod-cone dystrophies, cone-rod dystrophies and cone degenerations.
  • complement component refers to the molecules that are involved in activation of the complement system.
  • the classical pathway components include, e.g., C1 q, C1 r, C1 s, C4, C2, C3, C5, C6, C7, C8, C9, and C5b-9 complex (membrane attack complex: MAC).
  • the alternative pathway components include, e.g., Factor B, Factor D, Factor H, Factor I and Properdin.
  • cellular activities regulated by the complement pathway include cell damage resulting from the C5b-9 attack complex, vascular permeability changes, contraction and migration of smooth muscle cells, T cell proliferation, immune adherence, aggregation of dendritic cells, monocytes, granulocyte and platelet, phagocytosis, migration and activation of neutrophils (PMN) and macrophages.
  • activation of the complement pathways results in the increase of proinflammatory response contributed by the by-products within the complement pathway.
  • Disorders associated with activation of the complement pathway include nephritis, asthma, reperfusion injury, hemodialysis, rheumatoid arthritis, systemic lupus, psoriasis, multiple sclerosis, transplantation, Alzheimer's disease, aHUS, MPGN II, or any other complement-mediated disease.
  • disorders associated with macular degeneration include AMD, North Carolina macular dystrophy, Sorsby's fundus dystrophy, Stargardt's disease, pattern dystrophy, Best disease, dominant drusen, and malattia leventinese (radial drusen), extramacular changes that occur prior to, or following dysfunction and/or degeneration of the macula, retinal detachment, chorioretinal degenerations, retinal degenerations, photoreceptor degenerations, RPE degenerations, mucopolysaccharidoses, rod-cone dystrophies, cone-rod dystrophies and cone degenerations.
  • chimeric antibody is an antibody molecule in which (a) the constant region, or a portion thereof, is altered, replaced or exchanged so that the antigen binding site (variable region) is linked to a constant region of a different or altered class, effector function and/or species, or an entirely different molecule which confers new properties to the chimeric antibody, e.g., an enzyme, toxin, hormone, growth factor, drug, etc.; or (b) the variable region, or a portion thereof, is altered, replaced or exchanged with a variable region having a different or altered antigen specificity.
  • a mouse antibody can be modified by replacing its constant region with the constant region from a human immunoglobulin. Due to the replacement with a human constant region, the chimeric antibody can retain its specificity in recognizing the antigen while having reduced antigenicity in human as compared to the original mouse antibody.
  • human Factor P has the sequence as set out in Table 1 : SEQ ID NO: 401 .
  • Human Factor P can be obtained from Complement Tech, Tyler, TX.
  • Cynomolgus Factor P can be purified from cynomolgus serum (protocol adapted from Nakano et al., (1986) J Immunol Methods 90:77-83).
  • Examples of Factor P protein from other species are provided in Table 1 , SEQ ID NOs: 402, 403, 404 or 405, as well as Factor P protein binding domains and fragments (e.g.: SEQ ID NOs: 406, 407 or 408).
  • Factor P is also know in the art as "Properdin”.
  • conservatively modified variants refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide.
  • nucleic acid variations are "silent variations," which are one species of conservatively modified variations. Every nucleic acid sequence herein which encodes a polypeptide also describes every possible silent variation of the nucleic acid.
  • each codon in a nucleic acid except AUG, which is ordinarily the only codon for methionine, and TGG, which is ordinarily the only codon for tryptophan
  • TGG which is ordinarily the only codon for tryptophan
  • conservatively modified variants include individual substitutions, deletions or additions to a polypeptide sequence which result in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art. Such conservatively modified variants are in addition to and do not exclude polymorphic variants, interspecies homologs, and alleles of the invention.
  • the following eight groups contain amino acids that are conservative substitutions for one another: 1 ) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), Threonine (T); and 8) Cysteine (C), Methionine (M) (see, e.g., Creighton, Proteins (1984)).
  • the term "conservative sequence modifications” are used to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence.
  • epitope means a protein determinant capable of specific binding to an antibody.
  • Epitopes usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and nonconformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.
  • human antibody is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from sequences of human origin. Furthermore, if the antibody contains a constant region, the constant region also is derived from such human sequences, e.g., human germline sequences, or mutated versions of human germline sequences.
  • the human antibodies of the invention may include amino acid residues not encoded by human sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).
  • human monoclonal antibody refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human sequences.
  • the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
  • a “humanized” antibody is an antibody that retains the reactivity of a non-human antibody while being less immunogenic in humans. This can be achieved, for instance, by retaining the non-human CDR regions and replacing the remaining parts of the antibody with their human counterparts (i.e., the constant region as well as the framework portions of the variable region). See, e.g., Morrison et al., Proc. Natl. Acad. Sci. USA, 81 :6851 -6855, 1984; Morrison and Oi, Adv. Immunol., 44:65-92, 1988;
  • nucleic acids or polypeptide sequences refer to two or more sequences or subsequences that are the same.
  • Two sequences are “substantially identical” if two sequences have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 60% identity, optionally 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% identity over a specified region, or, when not specified, over the entire sequence), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection.
  • the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides (or 20, 50, 200 or more amino acids) in length.
  • sequence comparison typically one sequence acts as a reference sequence, to which test sequences are compared.
  • test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated.
  • sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program
  • a “comparison window”, as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned.
  • Methods of alignment of sequences for comparison are well known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith and Waterman (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol.
  • Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always > 0) and N (penalty score for mismatching residues; always ⁇ 0).
  • M forward score for a pair of matching residues; always > 0
  • N penalty score for mismatching residues; always ⁇ 0.
  • a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached.
  • the BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment.
  • W wordlength
  • E expectation
  • the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787, 1993).
  • One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance.
  • P(N) the smallest sum probability
  • a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01 , and most preferably less than about 0.001.
  • the percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4:1 1 -17, 1988) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.
  • the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. Mol, Biol.
  • nucleic acid sequences or polypeptides are substantially identical is that the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the antibodies raised against the polypeptide encoded by the second nucleic acid, as described below.
  • a polypeptide is typically substantially identical to a second polypeptide, for example, where the two peptides differ only by conservative
  • nucleic acid sequences are substantially identical.
  • the two molecules or their complements hybridize to each other under stringent conditions, as described below.
  • Yet another indication that two nucleic acid sequences are substantially identical is that the same primers can be used to amplify the sequence.
  • inhibitor (or inhibits) the alternative complement pathway refers to the ability of Factor P antibodies to interfere with activation of the alternative complement pathway.
  • inhibitor refers to a statistically significant decrease (i.e., p ⁇ 0.05) in alternative complement activation as measured by one or more assays as described herein, including MAC formation, hemolytic assay, or C3b deposition assay in a cell or subject following contact with an anti-Factor P antibody or fragment thereof as described herein relative to a control.
  • inhibitor or inhibits the alternative complement activation as measured by one or more assays as described herein, including MAC formation, hemolytic assay, or C3b deposition assay in a cell or subject following contact with an anti-Factor P antibody or fragment thereof as described herein relative to a control.
  • complement pathway can also refer to a clinically relevant improvement in visual function or retinal anatomy following treatment with an anti-Factor P antibody described herein in a patient diagnosed with age related macular degeneration as described below.
  • isolated antibody refers to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds Factor P is substantially free of antibodies that specifically bind antigens other than Factor P).
  • An isolated antibody that specifically binds Factor P may, however, have cross-reactivity to other antigens.
  • an isolated antibody may be substantially free of other cellular material and/or chemicals.
  • isotype refers to the antibody class (e.g., IgM, IgE, IgG such as lgG1 or lgG4) that is provided by the heavy chain constant region genes. Isotype also includes modified versions of one of these classes, where modifications have been made to alter the Fc function, for example, to enhance or reduce effector functions or binding to Fc receptors.
  • Kassoc or “Ka”, as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction
  • Kdis or “Kd,” as used herein, is intended to refer to the dissociation rate of a particular antibody-antigen interaction
  • K D is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e. Kd/Ka) and is expressed as a molar concentration (M).
  • K D values for antibodies can be determined using methods well established in the art. Methods for determining the K D of an antibody include measuring surface plasmon resonance using a biosensor system such as a Biacore ® system, or measuring affinity in solution by solution equilibrium titration (SET).
  • monoclonal antibody or “monoclonal antibody composition” as used herein refer to a preparation of antibody molecules of single molecular composition.
  • a monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.
  • nucleic acid is used herein interchangeably with the term
  • polynucleotide refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form.
  • the term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl
  • PNAs peptide-nucleic acids
  • degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081 , 1991 ; Ohtsuka et al., J. Biol. Chem. 260:2605-2608, 1985; and Rossolini et al., Mol. Cell. Probes 8:91 -98, 1994).
  • operably linked refers to a functional relationship between two or more polynucleotide (e.g., DNA) segments.
  • the term refers to the functional relationship of a transcriptional regulatory sequence to a transcribed sequence.
  • a promoter or enhancer sequence is operably linked to a coding sequence if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system.
  • promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acting.
  • some transcriptional regulatory sequences, such as enhancers need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.
  • the term, "optimized" means that a nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in the production cell or organism, generally a eukaryotic cell, for example, a cell of Pichia, a Chinese Hamster Ovary cell (CHO) or a human cell.
  • the optimized nucleotide sequence is engineered to retain completely or as much as possible the amino acid sequence originally encoded by the starting nucleotide sequence, which is also known as the "parental" sequence.
  • the optimized sequences herein have been engineered to have codons that are preferred in mammalian cells. However, optimized expression of these sequences in other eukaryotic cells or prokaryotic cells is also envisioned herein.
  • the amino acid sequences encoded by optimized nucleotide sequences are also referred to as optimized.
  • polypeptide and "protein” are used interchangeably herein to refer to a polymer of amino acid residues.
  • the terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers and non- naturally occurring amino acid polymer. Unless otherwise indicated, a particular polypeptide sequence also implicitly encompasses conservatively modified variants thereof.
  • recombinant human antibody includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from an animal (e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, antibodies isolated from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isolated by any other means that involve splicing of all or a portion of a human immunoglobulin gene, sequences to other DNA sequences.
  • Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences.
  • such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.
  • recombinant host cell refers to a cell into which a recombinant expression vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein.
  • subject includes human and non-human animals.
  • Non-human animals include all vertebrates (e.g.: mammals and non-mammals) such as, non-human primates (e.g.: cynomolgus monkey), sheep, dog, cow, chickens, amphibians, and reptiles. Except when noted, the terms "patient” or “subject” are used herein
  • cyno or “cynomolgus” refer to the cynomolgus monkey (Macaca fascicularis).
  • treating refers in one embodiment, to ameliorating the disease or disorder (i.e., slowing or arresting or reducing the development of the disease or at least one of the clinical symptoms thereof).
  • treating refers to alleviating or ameliorating at least one physical parameter including those which may not be discernible by the patient.
  • treating refers to modulating the disease or disorder, either physically, (e.g., stabilization of a discernible symptom), physiologically, (e.g., stabilization of a physical parameter), or both.
  • treating refers to preventing or delaying the onset or development or progression of the disease or disorder.
  • prevention as it relates to AMD means any action that prevents or slows a worsening in visual function, retinal anatomy, and/or an AMD disease parameter, as described below, in a patient at rist for said worsening.
  • treatment of AMD means any action that results in the improvement or preservation of visual function and/or reginal anatomy.
  • vector is intended to refer to a polynucleotide molecule capable of transporting another polynucleotide to which it has been linked.
  • plasmid refers to a circular double stranded DNA loop into which additional DNA segments may be ligated.
  • viral vector such as an adeno- associated viral vector (AAV, or AAV2), wherein additional DNA segments may be ligated into the viral genome.
  • AAV adeno- associated viral vector
  • Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors).
  • vectors e.g., non-episomal mammalian vectors
  • vectors can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome.
  • certain vectors are capable of directing the expression of genes to which they are operatively linked.
  • Such vectors are referred to herein as "recombinant expression vectors" (or simply, "expression vectors”).
  • expression vectors of utility in recombinant DNA techniques are often in the form of plasmids.
  • plasmid and vector may be used interchangeably as the plasmid is the most commonly used form of vector.
  • the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.
  • FIG 1 Depicts the Factor P binding site.
  • Firgure 1A illustrates the relative position TSR5 domain of Factor P and the TSR5 sequence fragments: A, B, C and D.
  • Figure 1 B shows the human TSR5 sequence aligned with the mouse sequence. Brackets indicate the sequence fragments of TSR5.
  • Figure 1 C illustrates the antibodies bind to region B of TSR5.
  • Figure 2 shows the results of a hemolytic assay demonstrating inhibition of the alternative complement pathway in 20% human serum.
  • Figure 3 shows an isobologram generated using the data from the hemolytic assay depicted in Figure 2.
  • Figure 4 shows the % inhibition of macrophage infiltrates in a mouse poly-IC model, comparing the inhibition of anti-FactorP and anti-C5 antibodies singly and in
  • Figure 5 shows an isobologram generated using the data from the poly-IC results depicted in Figure 4.
  • the present invention is based, in part, on the discovery of antibody molecules that specifically bind to both human and cynomolgus Factor P.
  • the invention relates to both full IgG format antibodies as well as antigen binding fragments thereof, such as Fab fragments (e.g., see antibodies NVS965-S, NVS962-S, NVS804 and NVS807 ).
  • the present invention provides antibodies that specifically bind to Factor P (e.g., human Factor P, cynomolgus Factor P, rat Factor P, rabbit Factor P), pharmaceutical compositions, production methods, and methods of use of such antibodies and compositions.
  • Factor P e.g., human Factor P, cynomolgus Factor P, rat Factor P, rabbit Factor P
  • pharmaceutical compositions e.g., human Factor P, cynomolgus Factor P, rat Factor P, rabbit Factor P
  • production methods e.g., production methods, and methods of use of such antibodies and compositions.
  • the present invention provides antibodies that specifically bind to Factor P.
  • the present invention provides antibodies that specifically bind to human, cynomolgus, rat and/or rabbit Factor P.
  • Antibodies of the invention include, but are not limited to, the human monoclonal antibodies and Fabs, isolated as described in the Examples.
  • the present invention provides antibodies that specifically bind a Factor P protein (e.g., human and/or cynmolgus Factor P), wherein the antibodies comprise a VH domain having an amino acid sequence of SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 or 273.
  • the present invention also provides antibodies that specifically bind to a Factor P protein, wherein the antibodies comprise a VH CDR having an amino acid sequence of any one of the VH CDRs listed in Table 1 , infra.
  • the invention provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), wherein the antibodies comprise (or alternatively, consist of) one, two, three, or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1 , infra.
  • a Factor P protein e.g., human and/or cynomolgus Factor P
  • the antibodies comprise (or alternatively, consist of) one, two, three, or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1 , infra.
  • the present invention provides antibodies that specifically bind to a Factor P protein, said antibodies comprising a VL domain having an amino acid sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274.
  • the present invention also provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), said antibodies comprising a VL CDR having an amino acid sequence of any one of the VL CDRs listed in Table 1 , infra.
  • the invention provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1 , infra.
  • a Factor P protein e.g., human and/or cynomolgus Factor P
  • said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1 , infra.
  • antibodies of the invention include amino acids that have been mutated, yet have at least 60, 70, 80, 85, 90 or 95 percent identity in the CDR regions with the CDR regions depicted in the sequences described in Table 1. In some embodiments, it includes mutant amino acid sequences wherein no more than 1 , 2, 3, 4 or 5 amino acids have been mutated in the CDR regions when compared with the CDR regions depicted in the sequence described in Table 1 .
  • the present invention also provides nucleic acid sequences that encode VH, VL, the full length heavy chain, and the full length light chain of the antibodies that specifically bind to a Factor P protein ⁇ e.g., human and/or cynomolgus Factor P).
  • a Factor P protein e.g., human and/or cynomolgus Factor P.
  • Such nucleic acid sequences can be optimized for expression in mammalian cells (for example, Table 1 shows the optimized nucleic acid sequences for the heavy chain and light chain of antibodies of the invention).
  • AMINO ACID SEQUENCE IDENTIFIER SEQ . ID . NO :
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • NINPVDGGTEYAQKFQG (Rabat) / NPVDGG (Chothia)
  • AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAACATCAACCCCGTGGACGGCGGCACCGAGTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCA
  • HGGYVFDS (Rabat) / HGGYVFDS (Chothia)
  • AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
  • HGGYVFDS (Rabat) / HGGYVFDS (Chothia)
  • AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia) CDRL1 172/356
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • PN ENCODING 193 SEQ. ID.NO: 189 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • VL 204 VL 204
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • VH 231 EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
  • Antibody 8109 Sequence Identifier (SEQ ID NO:)
  • Antibody 8110 Sequence Identifier (SEQ ID NO:) and Sequence or comments

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Abstract

The present invention relates to antibodies or antigen binding fragments thereof that bind to complement Factor P and used thereof as well as combinations of anti- Factor P antibodies with antibodies or antigen binding fragments thereof that bind to compelement component 5 (C5).

Description

COMPOSITIONS AND METHODS FOR ANTIBODIES TARGETING FACTOR P
BACKGROUND OF THE INVENTION
Age related macular degeneration (AMD) is a progressive disease and a leading cause of vision loss and blindness in Americans aged 65 and older. AMD primarily affects the macula; a part of the retina responsible for high visual acuity needed to read or drive. The majority of AMD patients suffer from an early stage of the disease which is characterized by the presence of extracellular retinal deposits called drusen. Drusen are extracellular retinal deposits of cell debris, inflammatory mediators, and extracellular matrix components. The late stages of AMD manifest as a dry or wet form, both are associated with vision loss. Dry AMD, also known as geographic atrophy, appears on ophthalmoscopic examination as clearly demarcated regions corresponding to local areas of retinal pigmented epithelium (RPE) loss. Wet AMD is associated with neovascularization of the choriod, causing a loss of integrity in Bruch's membrane and vessel growth in the retina, where they can often hemorrhage. This leakage causes permanent damage to retinal cells which die off and create blind spots in the central vision.
The innate human system is composed of the complement pathway. The complement pathway serves to defend against pyogenic bacterial infection bridging innate and adaptive immunity; and disposing of products of immune complexes and inflammatory injury. The complement is a system of more than 30 proteins involved in cascade reactions in plasma and cell surfaces. The complement system and its complement components are involved in various immune processes. For example, complement C5b-9 complex, also termed the terminal complex or the membrane attack complex (MAC), plays an important role in cell death by inducing membrane permeability damages.
There are three known complement activation pathways: the classical, lectin, and alternative pathways. All three pathways lead to the cleavage of C3 by C3 convertase and subsequent cleavage of C5 by the C5 convertase, releasing C3a, C5a, and C5b. Factor P is a key regulator of the alternative complement pathway. It is proposed to have two major functions in vivo. First, Factor P stabilizes the C3 and C5 convertases by binding to C3b of the convertase enzyme and thereby prolongs the half life of C3 convertase. Second, Factor P may determine which cells will be lysed by attaching to a cell surface and functioning as a template on which convertases can form, leading to activation of the alternative complement pathway and lysis of the cell. Recent work has demonstrated that complement components C3 and C5 are principal constituents of drusen in patients with AMD. Mulling, R.F. et al. (2000) FASEB J 14, 835-46 Their presence as well as that of the membrane attack complex (MAC) C5b-9 and other acute phase reactant proteins in RPE cells overlying drusen has been speculated to be involved in the process that can trigger complement activation and formation of MAC. Johnson, L et al. (2001 ) Exp Eye Res 73, 887-896. Thus, there is growing evidence that complement components are more than mere mediators of innate immunity.
Nutritional intervention has been prescribed to inhibit progression of dry AMD to wet AMD. At present the only FDA approved treatments for wet AMD include
photodynamic therapy (PDT), an anti-VEGF aptamer, such as pegaptanib, and anti- VEGF antibodies, ranibizumab. These drugs or therapies are typically administered to patients who have already suffered substantial vision loss.
There remains a need to develop an effective treatment for AMD, particularly dry AMD to replace or supplement current treatments. Particularly, there is a need for treatments which can provide early detection, prevention or restoration of vision loss.
SUMMARY OF THE INVENTION
The present invention relates to an isolated antibody, or antigen binding fragment thereof, that binds to human or cynomolgus Factor P, wherein said antibody binds to the TSR5 domain (SEQ ID NO: 406). For example, the antibodies, or antigen binding fragments described herein bind to a region of the TSR5 domain comprising the sequence of SEQ ID NO: 407, more specifically said antibodies also bind a region of the Factor P TSR5 domain comprising the amino acid sequence KSISC (SEQ ID NO: 408). In certain embodiments, the isolated antibodies, or antigen binding fragments thereof, bind to a Factor P epitope comprising the amino acid sequence of SEQ ID NO: 407. In other embodiments, the isolated antibodies, or antigen binding fragments thereof, bind to a Factor P epitope comprising the amino acid sequence of SEQ ID NO: 408.
The isolated antibodies, or antigen binding fragments, described herein bind Factor P, with a KD of less than or equal to 1 .2 nM. For example, the isolated antibodies or antigen binding fragments described herein may bind to human or cynomolgus Factor P with a KD of less than or equal to 1.1 nM, less than or equal to 1 nM, less than or equal to 600pM, less than or equal to 500 pM, less than or equal to 400 pM, less than or equal to 300 pM, less than or equal to 200 pM, less than or equal to 100 pM, less than or equal to 75 pM, less than or equal to 50 pM, less than or equal to 40pM, less than or equal to 30 pM, less than or equal to 20 pM, or less than or equal to 10pM.
The binding affinity of isolated antibodies and antigen binding fragments described herein can be determined by solution equilibrium titration (SET). Methods for SET are known in the art and are described in further detail below. Alternatively, binding affinity of the isolated antibodies, or fragments, described herein can be determined by Biacore assay. Methods for Biacore kinetic assays are know in the art and are described in further detail below.
The isolated antibodies and antigen binding fragments described herein can be used to inhibit the alternative complement pathway. For example, an isolated antibody or antigen binding fragment thereof can inhibit the alternative complement pathway as measure by an in vitro hemolytic assay with an IC50 of less than or equal to 25 nm, less than or equal to 20 nM, less than or equal to 16nM, less than or equal to 15nM, less than or equal to 14nM, less than or equal to 13nM, less than or equal to 12nM, less than or equal to 1 1 nM, less than or equal to 10nM, less than or equal to 9nM, less than or equal to 8nM, less than or equal to 7nM. More specifically, an isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway in human as measure by an in vitro hemolytic assay with an IC50 of less than or equal to 16 nm, or less than or equal to 9 nm.
An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway as measure by an in vitro C3b deposition assay with an IC50 of less than or equal to 10 nm, less than or equal to 7nM, less than or equal to 6 nM, less than or equal to 5nM, less than or equal to 4 nM, less than or equal to 3 nM, less than or equal to 2 nM, less than or equal to 1 nM, less than or equal to 15nM, less than or equal to 1 nM, less than or equal to 0.5 nM, or less than or equal to 0.1 nM. More specifically, an isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway in human as measure by an in vitro C3b deposition assay with an IC50 of less than or equal to 3 nm, or less than or equal to 2 nM.
An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway with an IC50 of less than or equal to 25 nm, less than or equal to 20 nM, less than or equal to 15 nM, less than or equal to 10 nM, less than or equal to 9 nM, less than or equal to 8 nM, less than or equal to 7 nM, or less than or equal to 6 nM, as measure by deposition of the complement membrane attack complex. More specifically, an isolated antibody or fragment thereof as described herein can inhibit the alternative complement pathway in human with an IC50 of less than or equal to 25 nm, or less than or equal to 7.5 nM, as measure by deposition of the complement membrane attack complex.
An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C3a.
An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of iC3b.
An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C5a.
An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C5b.
An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway by destabilizing and/or blocking the activity of C3 and/or C5 convertase, as measured by a decrease in production of C3a, C3b, iC3b, C5a, and/or C5b.
An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the generation of C5a with an IC50 of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM.
The isolated antibodies, or antigen binding fragment thereof, may also block Factor P binding to C3b and/or prevent Factor P binding to the cell surface or to DNA or oligonucleotides.
Another aspect of the invention includes an isolated antibody, or antigen binding fragment thereof, that specifically binds to human, cynomolgus, rat and/or rabbit Factor P. In a further aspect, the isolated antibody, or antigen binding fragment, competes for binding with an antibody, or antigen binding fragment, described in Table 1.
The isolated antibodies, or antigen binding fragments thereof, as described herein can be a monoclonal antibodies, a human or humanized antibodies, a chimeric antibodies, single chain antibodies, Fab fragments, Fv fragments, F(ab')2 fragments, or ScFv fragments, and/or IgG isotypes.
The isolated antibodies, or antigen binding fragments thereof, as described herein can also include a framework in which an amino acid has been substituted into the antibody framework from the respective human VH or VL germline sequences.
Another aspect of the invention includes an isolated antibody or antigen binding fragment thereof having the heavy and light chain sequences of Fabs described in Table 1 . For example, the isolated antibody or antigen binding fragment thereof can have the heavy and light chain sequences of Fab NVS962, NVS963, NVS964, NVS965, NVS966, NVS967, NVS962-G, NVS962-S, NVS962-T, NVS962-Q, NVS962-S31A, NVS965-Q, NVS965-S, NVS965-T, NVS804, NVS805, NVS806, NVS807, or NVS808.
A further aspect of the invention includes an isolated antibody or antigen binding fragment thereof having the heavy and light chain variable domain sequences of Fabs described in Table 1 . For example, the isolated antibody or antigen binding fragment there of can have the heavy and light chain variable domain sequence of Fab NVS962 , NVS963, NVS964, NVS965, NVS966, NVS967, NVS962-G, NVS962-S, NVS962-T, NVS962-Q, NVS962-S31A, NVS965-Q, NVS965-S, NVS965-T, NVS804, NVS805, NVS806, NVS807, or NVS808.
The invention also relates to an isolated antibody or antigen binding fragment thereof that includes a heavy chain CDR1 selected from the group consisting of SEQ ID NOs 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, and 267; a heavy chain CDR2 selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268; and a heavy chain CDR3 selected from the group consisting of SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, and 269, wherein the isolated antibody or antigen binding fragment thereof binds to human Factor P. In another aspect, the isolated antibody or antigen binding fragment thereof further includes a light chain CDR1 selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; a light chain CDR2 selected from the group consisting of SEQ ID NOs 5, 19, 33, 47, 61 , 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271; and a light chain CDR3 selected from the group consisting of SEQ ID NOs 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272.
The invention also relates to an isolated antibody or antigen binding fragment thereof that includes a light chain CDR1 selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; a light chain CDR2 selected from the group consisting of SEQ ID NOs 5, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271 ; and a light chain CDR3 selected from the group consisting of SEQ ID NOs 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202,216, 230, 244, 258, and 272, wherein the isolated antibody or antigen binding fragment thereof binds to human Factor P.
The invention also relates to an isolated antibody or antigen binding fragment thereof that binds Factor P having HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs: 1, 2, 3, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 4, 5, 6; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 15, 16, 17, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 18, 19, 20; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 29, 30, 31, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 32, 33, 34; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 43, 44, 45, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 46, 47, 48; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 57, 58, 59, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 60, 61, 62; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 71, 72, 73, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 74, 75, 76; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 85, 86, 87, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 88, 89, 90; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 99, 100, 101, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 102, 103, 104; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 113, 114, 115, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 116, 117, 118; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 127, 128, 129, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 130, 131 , 132; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 141 , 142, 143, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 144, 145, 146; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 155, 156, 157, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 158, 159, 160; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 169, 170, 171 , and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 172, 173, 174; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 183, 184, 185, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 186, 187, 188; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 197, 198, 199, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 200, 201 , 202; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 21 1 , 212, 213, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 214, 215, 216; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 225, 226, 227, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 228, 229, 230; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 239, 240, 241 , and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 242, 243, 244; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 253, 254, 255, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 256, 257, 258; or HCDR1 , HCDR2, HCDR3 and LCDR1 , LCDR2, LCDR3, wherein HCDR1 , HCDR2, HCDR3 comprises SEQ ID NOs 267, 268, 269, and LCDR1 , LCDR2, LCDR3 comprises SEQ ID NOs: 270, 271 , 272.
In one embodiment of the invention the isolated antibody or antigen binding fragment thereof includes a heavy chain variable domain sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273. In another embodiment , the isolated antibody or antigen binding fragment further comprises a light chain variable domain seqeunce wherein the heavy chain variable domain and light chain variable domain combine to form and antigen binding site for Factor P. In a further embodiment the isolated antibody or antigen binding fragment further includes a light chain variable domain sequence selected from SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274 wherein said isolated antibody or antigen binding fragment thereof binds Factor P. The invention also relates to an isolated antibody or antigen binding fragment thereof that includes a light chain variable domain sequence selected from the group consisting of SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, wherein said isolated antibody or antigen binding fragment thereof binds to human Factor P. In one embodiment , the isolated antibody or antigen binding fragment further comprises a heavy chain variable domain sequence wherein the light chain variable domain and heavy chain variable domain combine to form and antigen binding site for Factor P.
In another embodiment of the invention, the isolated antibody or antigen binding fragment thereof that binds Factor P, may have heavy and light chain variable domains comprising the sequences of SEQ ID NOs: 7 and 8; 21 and 22; 35 and 36; 49 and 50; 63 and 64; 77 and 78; 91 and 92; 104 and 105; 1 18 and 1 19; 132 and 133; 146 and 147; 160 and 161 ; 174 and 175; 188 and 189; 202 and 203; 216 and 217; 230 and 231 ; 244 and 245; 258 and 259; or 272 and 273, respectively.
The invention further relates to an isolated antibody or antigen binding fragment thereof, that includes a heavy chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273, wherein said antibody binds to Factor P. In one aspect, the isolated antibody or antigen binding fragment thereof also includes a light chain variable domain having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274.
In another embodiment the isolated antibody or antigen binding fragment thereof, may include a light chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, wherein said antibody binds Factor P.
In another ambodiment the isolated antibody, or antigen binding fragment thereof, that binds to Factor P may have a heavy chain comprising the sequence of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275. In a further embodiment, the isolated antibody also includes a light chain that can combine with the heavy chain to form an antigen binding site to human Factor P. In a further embodiment, the isolated antibody or antigen binding fragment thereof includes a light chain having a sequence comprising SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276.
The invention still further relates to an isolated antibody or antigen binding fragment thereof that includes a heavy chain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 9, 23, 37, 51 , 65, 79, 93,
107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 and 275, wherein said antibody binds to Factor P. In one aspect, the isolated antibody or antigen binding fragment thereof also includes a light chain having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 10, 24, 38, 52, 66, 80, 94,
108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, and 276.
The invention still further relates to an isolated antibody or antigen binding fragment thereof that includes a light chain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 and 275, wherein said antibody binds Factor P.
The invention also relates to compositions comprising the isolated antibody, or antigen binding fragment thereof, described herein. As well as, antibody compositions in combination with a pharmaceutically acceptable carrier. Specifically, the invention further includes pharmaceutical compositions comprising an antibody or antigen binding fragment thereof of Table 1 , such as, for example antibody NVS962, NVS963, NVS964, NVS965, NVS966, NVS967, NVS962-G, NVS962-S, NVS962-T, NVS962-Q, NVS962- S31A, NVS965-Q, NVS965-S, NVS965-T, NVS804, NVS805, NVS806, NVS807, or NVS808. The invention also realtes to pharmaceutical compositions comprising a combination of two or more of the isolated antibodies or antigen binding fragments thereof of Table 1.
The invention also relates to an isolated nucleic acid comprising a sequence encoding a polypeptide that includes a heavy chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273.
The invention also relates to an isolated nucleic acid comprising a sequence encoding a polypeptide that includes a light chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274.
The invention also relates to a vector that includes one or more of the nucleic acid molecules described herein.
The invention also relates to an isolated host cell that includes a recombinant DNA sequence encoding a heavy chain of the antibody described above, and a second recombinant DNA sequence encoding a light chain of the antibody described above, wherein said DNA sequences are operably linked to a promoter and are capable of being expressed in the host cell. It is contemplated that the antibody can be a human monoclonal antibody. It is also contemplated that the host cell is a non-human mammalian cell.
The invention also relates to a method of inhibiting the complement mediated cell death wherein the method includes the step of contacting a cell with an effective amount of a composition comprising the isolated antibody or antigen binding fragments thereof described herein. It is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.
The invention still further relates to a method of inhibiting the alternative complement pathway in a cell wherein the method includes the step of contacting the cell with an effective amount of a composition comprising the isolated antibody or antigen binding fragments thereof described herein. In one aspect, it is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.
The invention also relates to a method of inhibiting the formation of membrane attack complex in a cell wherein the method includes the step of contacting the cell with an effective amount of a composition comprising the isolated antibody or antigen binding fragments thereof described herein. It is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.
Any of the foregoing isolated antibodies or antigen binding fragments thereof may be a monoclonal antibody or antigen binding fragment thereof. In one aspect, the invention provides a a first antibody, or antigen binding fragment thereof, that binds Factor P, and a second antibody, or antigen binding fragment thereof, that binds C5, wherein said combination inhibits the alternative complement pathway. In one aspect the first and second antibodies can be in combination as a composition.
Such a combination can be used to inhibit ocular inflammation. Ocular inflammation can be determined by measuring neutrophil accumulation and/or macrophage recruitment in the retina.
In one aspect, such a combination can be used to inhibit neutrophil accumulation in the retina, or macrophage recruitment in the retina.
In one aspect, the antibody in such a combination that binds Factor P, binds a region of Factor P comprising SEQ ID NO: 408. Alternatively or in combination, such an antibody binds a region of Factor P comprising SEQ ID NO: 407.
In a further aspect, the combination of antibodies or binding fragments thereof that bind Factor P and C5 include a first antibody or antigen binding fragment selected from Table 1 and a second antibody or antigen-binding fragment selected from Table 2. In one aspect, the first antibody, or antigen binding fragment thereof binds the same epitope as is an antibody described in Table 1 and the second antibody, or antigen binding fragment thereof, binds the same epitope as is an antibody described in Table 2.
In one aspect, the invention provides a first antibody, or antigen binding fragment thereof that comprises a heavy chain CDR1 , 2, 3, and a light chain CDR1 , 2, 3, selected from the group consisting of a) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 , 2, and 3, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6,
respectively; b) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 15, 16, and 17, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 18, 19, and 20, respectively; c) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 29, 30, and 31 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 32, 33, and 34, respectively; d) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 43, 44, and 45, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 46, 47, and 48, respectively; e) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 57, 58, and 59, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 60, 61 , and 62, respectively; f) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 71 , 72, and 73, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 74, 75, and 76, respectively; g) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 85, 86, and 87, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 88, 89, and 90, respectively; h) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 99, 100, and 101 ,
respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 102, 103, and 104, respectively; i) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 13, 1 14, and 1 15, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 1 16, 1 17, and 1 18, respectively; j) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 127, 128, and 129, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 130, 131 , and 132, respectively; k) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 141 , 142, and 143, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 144, 145, and 146, respectively; I) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 155, 156, and 157, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 158, 159, and 160, respectively; m) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 169, 170, and 171 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 172, 173, and 174, respectively; n) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 183, 184, and 185, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 186, 187, and 188, respectively; o) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 197, 198, and 199, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 200, 201 , and 202, respectively; p) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 21 1 , 212, and 213, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 214, 215, and 216, respectively; q) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 225, 226, and 227, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 228, 229, and 230, respectively; r) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 239, 240, and 241 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 242, 243, and 244, respectively; s) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 253, 254, and 255, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 256, 257, and 258, respectively; and t) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 267, 268, and 269, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 270, 271 , and 272, respectively, and wherein the second antibody or antigen binding fragment thereof comprises a heavy chain CDR1 , 2, 3 and light chain CDR1 , 2, 3 selected from the group consisting of: a) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 410, 41 1 , and 412, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 413, 414, and 415, respectively; b) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 426, 427, and 428, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 429, 430, and 431 , respectively; c) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 442, 443, and 444, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 445, 446, and 447, respectively; d) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 426, 458, and 428, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 429, 430, and 459, respectively; and e) a heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 470, 471 , and 472, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 473, 474 and 475,
respectively.
In one aspect, the invention relates to a first and second antibody or antigen bidning fragement thereof (which may be in combination as a composition) where the first antibody or antigen binding fragment thereof includes heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 7 and 8; SEQ ID NOs: 21 and 22; SEQ ID NOs: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID NOs: 105 and 106; SEQ ID NOs: 1 19 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID NOs: 161 and 162; SEQ ID NOs: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID NOs: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID NOs: 231 and 232; SEQ ID NOs: 245 and 246; SEQ ID NOs: 259 and 260; or SEQ ID NOs: 273 and 274, respectively, and wherein the second antibody or antigen binding fragment thereof includes heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 416 and 417; SEQ ID NOs: 432 and 433; SEQ ID NOs: 448 and 449; SEQ ID NOs: 460 and 461 ; or SEQ ID NOs: 476 and 477, respectively.
In one aspect, the invention relates to a first and second antibody or antigen bidning fragement thereof (which may be in combination as a composition) where the first antibody or antigen binding fragment thereof includes heavy and light chain variable regions having amino acid sequences selected from SEQ ID NOs: 7 and 8; SEQ ID NOs: 21 and 22; SEQ ID NOs: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID NOs: 105 and 106; SEQ ID NOs: 1 19 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID NOs: 161 and 162; SEQ ID NOs: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID NOs: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID NOs: 231 and 232; SEQ ID NOs: 245 and 246; SEQ ID NOs: 259 and 260; or SEQ ID NOs: 273 and 274, respectively, and wherein the second antibody or antigen binding fragment thereof includes heavy and light chain variable regions having amino acid sequences selected from SEQ ID NOs: 416 and 417; SEQ ID NOs: 432 and 433; SEQ ID NOs: 448 and 449; SEQ ID NOs: 460 and 461 ; or SEQ ID NOs: 476 and 477, respectively.
In a further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereo includes a heavy chain variable region comprising SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259, or 273 and further includes a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a heavy chain variable region comprising SEQ ID NO: 416, 432, 448, 460 or 476 and further includes a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to C5. In a further aspect, the first antibody or antigen binding fragment thereof includes the light chain variable region sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274, and the second antibody or antigen binding fragment thereof includes the light chain variable region sequence of SEQ ID NO: 417, 433, 449, 461 or 477.
In a further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody or antigen bidning fragment thereof incldues a light chain variable domain comprising SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274 and further includes a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a light chain variable region comprises a light chain variable domain includes SEQ ID NO: 417, 433, 449, 461 or 477 and further comprises a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to C5.
In one aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further includes a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to C5. In a further aspect, the first antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276, and wherein the second antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 419, 435, 451 , 463, or 479.
In one aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereof includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and further includes a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 419, 435, 451 , 463, or 479 and further includes a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to C5.
In one aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further includes a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second antibody or antigen binding fragement thereof includes a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 419, 435, 451 , 463, or 479.
In a further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain with an amino acid sequence seclected from SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further includes a light chain with an amino acid sequence selected from SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second antibody or antigen binding fragement thereof includes a heavy chain with an amino acid sequence selected from SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain with an amino acid sequence selected from SEQ ID NO: 419, 435, 451 , 463, or 479.
In a further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity, respectively, to SEQ ID NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52, 65 and 66, 79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity, respectively, to SEQ ID NOs: 418 and 419, 434 and 435; 450 and 451 ; 462 and 463; or 478 and 479.
In a still further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence, respectively, selected from SEQ ID NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52, 65 and 66, 79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence, respectively, selected from SEQ ID NOs: 418 and 419, 434 and 435; 450 and 451 ; 462 and 463; or 478 and 479.
The invention further relates to an isolated nucleic acid molecule comprising a nucleotide sequence encoding the first and/or second antibody or antigen binding fragment thereof as described herein. Such a nucleic acid sequence can be included in a vector, which may, in turn be included in a host cell which, in one aspect, is capable of expressing such nucleic acid sequence.
The invention further relates to a method of treating age related macular degeneration in a subject comprising administering to said subject, an effective amount of a first and second antibody or antigen binding fragment thereof, either singly, or in combination as a composition. The subject may be a human.
The invention further releates to a method of inhibiting the alternative
complement pathway in a subject comprising administering to said subject an effective amount of a first and second antibody or antigen binding fragment thereof, either singly, or in combination as a composition. The subject may be a human.
DEFINITIONS
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this invention pertains.
The term "antibody" as used herein means a whole antibodies and any antigen binding fragment (/'. e., "antigen-binding portion") or single chains thereof. A whole antibody is a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds. Each heavy chain is comprised of a heavy chain variable region (abbreviated herein as VH) and a heavy chain constant region. The heavy chain constant region is comprised of three domains, CH1 , CH2 and CH3. Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region. The light chain constant region is comprised of one domain, CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs arranged from amino-terminus to carboxy- terminus in the following order: FR1 , CDR1 , FR2, CDR2, FR3, CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an antigen. The constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the classical complement system.
The term "antigen binding portion" or "antigen binding fragment" of an antibody, as used herein, refers to one or more fragments of an intact antibody that retain the ability to specifically bind to a given antigen (e.g., Factor P). Antigen binding functions of an antibody can be performed by fragments of an intact antibody. Examples of binding fragments encompassed within the term antigen binding portion or antigen binding fragment of an antibody include a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; a F(ab)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; an Fd fragment consisting of the VH and CH1 domains; an Fv fragment consisting of the VL and VH domains of a single arm of an antibody; a single domain antibody (dAb) fragment (Ward et al., 1989 Nature 341 :544-546), which consists of a VH domain or a VL domain; and an isolated complementarity determining region (CDR).
Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by an artificial peptide linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see, e.g., Bird et al., 1988 Science 242:423-426; and Huston et al., 1988 Proc. Natl. Acad. Sci. 85:5879-5883). Such single chain antibodies include one or more antigen binding portions or fragments of an antibody. These antibody fragments are obtained using conventional techniques known to those of skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.
Antigen binding fragments can also be incorporated into single domain antibodies, maxibodies, minibodies, intrabodies, diabodies, triabodies, tetrabodies, v- NAR and bis-scFv (see, e.g., Hollinger and Hudson, 2005, Nature Biotechnology, 23, 9, 1 126-1 136). Antigen binding portions of antibodies can be grafted into scaffolds based on polypeptides such as Fibronectin type III (Fn3) (see U.S. Pat. No. 6,703,199, which describes fibronectin polypeptide monobodies).
Antigen binding fragments can be incorporated into single chain molecules comprising a pair of tandem Fv segments (VH-CH1 -VH-CH1 ) which, together with complementary light chain polypeptides, form a pair of antigen binding regions (Zapata et a/., 1995 Protein Eng. 8(10):1057-1062; and U.S. Pat. No. 5,641 ,870).
As used herein, the term "affinity" refers to the strength of interaction between antibody and antigen at single antigenic sites. Within each antigenic site, the variable region of the antibody "arm" interacts through weak non-covalent forces with antigen at numerous sites; the more interactions, the stronger the affinity. As used herein, the term "high affinity" for an IgG antibody or fragment thereof (e.g., a Fab fragment) refers to an antibody having a KD of 10"8 M or less, 10"9 M or less, or 10"10 M, or 10"11 M or less, or 10"12 M or less, or 10"13 M or less for a target antigen. However, high affinity binding can vary for other antibody isotypes. For example, high affinity binding for an IgM isotype refers to an antibody having a KD of 10"7 M or less, or 10"8 M or less.
The term "amino acid" refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids. Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, γ-carboxyglutamate, and O-phosphoserine. Amino acid analogs refer to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an alpha carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, e.g., homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid. Amino acid mimetics refers to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but that functions in a manner similar to a naturally occurring amino acid.
The term "binding specificity" as used herein refers to the ability of an individual antibody combining site to react with only one antigenic determinant.
The phrase "specifically (or selectively) binds" to an antibody (e.g., a Factor P- binding antibody) refers to a binding reaction that is determinative of the presence of a cognate antigen (e.g., a human Factor P or cynomolgus Factor P) in a heterogeneous population of proteins and other biologies. The phrases "an antibody recognizing an antigen" and "an antibody specific for an antigen" are used interchangeably herein with the term "an antibody which binds specifically to an antigen".
The term "conditions or disorders associated with macular degeneration" refers to any of a number of conditions in which the retinal macula degenerates or becomes dysfunctional, e.g., as a consequence of decreased growth of cells of the macula, increased death or rearrangement of the cells of the macula (e.g., RPE cells), loss of normal biological function, or a combination of these events. Macular degeneration results in the loss of integrity of the histoarchitecture of the cells and/or extracellular matrix of the normal macula and/or the loss of function of the cells of the macula.
Examples of macular degeneration-related disorder include AMD, North Carolina macular dystrophy, Sorsby's fundus dystrophy, Stargardt's disease, pattern dystrophy, Best disease, dominant drusen, and malattia leventinese (radial drusen). The term also encompasses extramacular changes that occur prior to, or following dysfunction and/or degeneration of the macula. Thus, the term "macular degeneration-related disorder" also broadly includes any condition which alters or damages the integrity or function of the macula (e.g., damage to the RPE or Bruch's membrane). For example, the term encompasses retinal detachment, chorioretinal degenerations, retinal degenerations, photoreceptor degenerations, RPE degenerations, mucopolysaccharidoses, rod-cone dystrophies, cone-rod dystrophies and cone degenerations.
The term "complement component", "complement proteins" or "complement component proteins" refers to the molecules that are involved in activation of the complement system. The classical pathway components include, e.g., C1 q, C1 r, C1 s, C4, C2, C3, C5, C6, C7, C8, C9, and C5b-9 complex (membrane attack complex: MAC). The alternative pathway components include, e.g., Factor B, Factor D, Factor H, Factor I and Properdin.
The term "cellular activities regulated by the complement pathway" include cell damage resulting from the C5b-9 attack complex, vascular permeability changes, contraction and migration of smooth muscle cells, T cell proliferation, immune adherence, aggregation of dendritic cells, monocytes, granulocyte and platelet, phagocytosis, migration and activation of neutrophils (PMN) and macrophages.
Further, activation of the complement pathways results in the increase of proinflammatory response contributed by the by-products within the complement pathway. Disorders associated with activation of the complement pathway include nephritis, asthma, reperfusion injury, hemodialysis, rheumatoid arthritis, systemic lupus, psoriasis, multiple sclerosis, transplantation, Alzheimer's disease, aHUS, MPGN II, or any other complement-mediated disease. Disorders associated with macular degeneration include AMD, North Carolina macular dystrophy, Sorsby's fundus dystrophy, Stargardt's disease, pattern dystrophy, Best disease, dominant drusen, and malattia leventinese (radial drusen), extramacular changes that occur prior to, or following dysfunction and/or degeneration of the macula, retinal detachment, chorioretinal degenerations, retinal degenerations, photoreceptor degenerations, RPE degenerations, mucopolysaccharidoses, rod-cone dystrophies, cone-rod dystrophies and cone degenerations.
The term "chimeric antibody" is an antibody molecule in which (a) the constant region, or a portion thereof, is altered, replaced or exchanged so that the antigen binding site (variable region) is linked to a constant region of a different or altered class, effector function and/or species, or an entirely different molecule which confers new properties to the chimeric antibody, e.g., an enzyme, toxin, hormone, growth factor, drug, etc.; or (b) the variable region, or a portion thereof, is altered, replaced or exchanged with a variable region having a different or altered antigen specificity. For example, a mouse antibody can be modified by replacing its constant region with the constant region from a human immunoglobulin. Due to the replacement with a human constant region, the chimeric antibody can retain its specificity in recognizing the antigen while having reduced antigenicity in human as compared to the original mouse antibody.
The term "Factor P protein" or "Factor P antigen" or "Factor P" are used
interchangeably, and refers to the Factor P protein in different species. For example, human Factor P has the sequence as set out in Table 1 : SEQ ID NO: 401 . Human Factor P can be obtained from Complement Tech, Tyler, TX. Cynomolgus Factor P can be purified from cynomolgus serum (protocol adapted from Nakano et al., (1986) J Immunol Methods 90:77-83). Examples of Factor P protein from other species are provided in Table 1 , SEQ ID NOs: 402, 403, 404 or 405, as well as Factor P protein binding domains and fragments (e.g.: SEQ ID NOs: 406, 407 or 408). Factor P is also know in the art as "Properdin".
The term "conservatively modified variant" applies to both amino acid and nucleic acid sequences. With respect to particular nucleic acid sequences, conservatively modified variants refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide. Such nucleic acid variations are "silent variations," which are one species of conservatively modified variations. Every nucleic acid sequence herein which encodes a polypeptide also describes every possible silent variation of the nucleic acid. One of skill will recognize that each codon in a nucleic acid (except AUG, which is ordinarily the only codon for methionine, and TGG, which is ordinarily the only codon for tryptophan) can be modified to yield a functionally identical molecule. Accordingly, each silent variation of a nucleic acid that encodes a polypeptide is implicit in each described sequence.
For polypeptide sequences, "conservatively modified variants" include individual substitutions, deletions or additions to a polypeptide sequence which result in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art. Such conservatively modified variants are in addition to and do not exclude polymorphic variants, interspecies homologs, and alleles of the invention. The following eight groups contain amino acids that are conservative substitutions for one another: 1 ) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), Threonine (T); and 8) Cysteine (C), Methionine (M) (see, e.g., Creighton, Proteins (1984)). In some embodiments, the term "conservative sequence modifications" are used to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence.
The term "epitope" means a protein determinant capable of specific binding to an antibody. Epitopes usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and nonconformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.
The term "human antibody", as used herein, is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from sequences of human origin. Furthermore, if the antibody contains a constant region, the constant region also is derived from such human sequences, e.g., human germline sequences, or mutated versions of human germline sequences. The human antibodies of the invention may include amino acid residues not encoded by human sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). The term "human monoclonal antibody" refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human sequences. In one embodiment, the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.
A "humanized" antibody is an antibody that retains the reactivity of a non-human antibody while being less immunogenic in humans. This can be achieved, for instance, by retaining the non-human CDR regions and replacing the remaining parts of the antibody with their human counterparts (i.e., the constant region as well as the framework portions of the variable region). See, e.g., Morrison et al., Proc. Natl. Acad. Sci. USA, 81 :6851 -6855, 1984; Morrison and Oi, Adv. Immunol., 44:65-92, 1988;
Verhoeyen et al., Science, 239:1534-1536, 1988; Padlan, Molec. Immun., 28:489-498, 1991 ; and Padlan, Molec. Immun., 31 :169-217, 1994. Other examples of human engineering technology include, but are not limited to Xoma technology disclosed in US 5,766,886.
The terms "identical" or percent "identity," in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same. Two sequences are "substantially identical" if two sequences have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 60% identity, optionally 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% identity over a specified region, or, when not specified, over the entire sequence), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Optionally, the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides (or 20, 50, 200 or more amino acids) in length.
For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program
parameters.
A "comparison window", as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are well known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith and Waterman (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 48:443, 1970, by the search for similarity method of Pearson and Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444, 1988, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wl), or by manual alignment and visual inspection (see, e.g., Brent et al., Current Protocols in Molecular Biology, John Wiley & Sons, Inc. (Ringbou ed., 2003)).
Two examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., Nuc. Acids Res. 25:3389-3402, 1977; and Altschul et al., J. Mol. Biol. 215:403-410, 1990, respectively. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased.
Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always > 0) and N (penalty score for mismatching residues; always < 0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 1 1 , an expectation (E) or 10, M=5, N=-4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915, 1989) alignments (B) of 50, expectation (E) of 10, M=5, N=-4, and a comparison of both strands.
The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787, 1993). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01 , and most preferably less than about 0.001.
The percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4:1 1 -17, 1988) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. Mol, Biol. 48:444-453, 1970) algorithm which has been incorporated into the GAP program in the GCG software package (available on the world wide web at gcg.com), using either a Blossom 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1 , 2, 3, 4, 5, or 6.
Other than percentage of sequence identity noted above, another indication that two nucleic acid sequences or polypeptides are substantially identical is that the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the antibodies raised against the polypeptide encoded by the second nucleic acid, as described below. Thus, a polypeptide is typically substantially identical to a second polypeptide, for example, where the two peptides differ only by conservative
substitutions. Another indication that two nucleic acid sequences are substantially identical is that the two molecules or their complements hybridize to each other under stringent conditions, as described below. Yet another indication that two nucleic acid sequences are substantially identical is that the same primers can be used to amplify the sequence. The term "inhibit (or inhibits) the alternative complement pathway" refers to the ability of Factor P antibodies to interfere with activation of the alternative complement pathway. Specifically, "inhibit" refers to a statistically significant decrease (i.e., p<0.05) in alternative complement activation as measured by one or more assays as described herein, including MAC formation, hemolytic assay, or C3b deposition assay in a cell or subject following contact with an anti-Factor P antibody or fragment thereof as described herein relative to a control. As used herein, "inhibit (or inhibits) the alternative
complement pathway" can also refer to a clinically relevant improvement in visual function or retinal anatomy following treatment with an anti-Factor P antibody described herein in a patient diagnosed with age related macular degeneration as described below.
The term "isolated antibody" refers to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds Factor P is substantially free of antibodies that specifically bind antigens other than Factor P). An isolated antibody that specifically binds Factor P may, however, have cross-reactivity to other antigens. Moreover, an isolated antibody may be substantially free of other cellular material and/or chemicals.
The term "isotype" refers to the antibody class (e.g., IgM, IgE, IgG such as lgG1 or lgG4) that is provided by the heavy chain constant region genes. Isotype also includes modified versions of one of these classes, where modifications have been made to alter the Fc function, for example, to enhance or reduce effector functions or binding to Fc receptors.
The term "Kassoc" or "Ka", as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction, whereas the term "Kdis" or "Kd," as used herein, is intended to refer to the dissociation rate of a particular antibody-antigen interaction. The term "KD", as used herein, is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e. Kd/Ka) and is expressed as a molar concentration (M). KD values for antibodies can be determined using methods well established in the art. Methods for determining the KD of an antibody include measuring surface plasmon resonance using a biosensor system such as a Biacore® system, or measuring affinity in solution by solution equilibrium titration (SET).
The terms "monoclonal antibody" or "monoclonal antibody composition" as used herein refer to a preparation of antibody molecules of single molecular composition. A monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope. The term "nucleic acid" is used herein interchangeably with the term
"polynucleotide" and refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form. The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl
phosphonates, 2-O-methyl ribonucleotides, peptide-nucleic acids (PNAs).
Unless otherwise indicated, a particular nucleic acid sequence also implicitly
encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences, as well as the sequence explicitly indicated. Specifically, as detailed below, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081 , 1991 ; Ohtsuka et al., J. Biol. Chem. 260:2605-2608, 1985; and Rossolini et al., Mol. Cell. Probes 8:91 -98, 1994).
The term "operably linked" refers to a functional relationship between two or more polynucleotide (e.g., DNA) segments. Typically, the term refers to the functional relationship of a transcriptional regulatory sequence to a transcribed sequence. For example, a promoter or enhancer sequence is operably linked to a coding sequence if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system. Generally, promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis-acting. However, some transcriptional regulatory sequences, such as enhancers, need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.
As used herein, the term, "optimized" means that a nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in the production cell or organism, generally a eukaryotic cell, for example, a cell of Pichia, a Chinese Hamster Ovary cell (CHO) or a human cell. The optimized nucleotide sequence is engineered to retain completely or as much as possible the amino acid sequence originally encoded by the starting nucleotide sequence, which is also known as the "parental" sequence. The optimized sequences herein have been engineered to have codons that are preferred in mammalian cells. However, optimized expression of these sequences in other eukaryotic cells or prokaryotic cells is also envisioned herein. The amino acid sequences encoded by optimized nucleotide sequences are also referred to as optimized.
The terms "polypeptide" and "protein" are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid polymers and non- naturally occurring amino acid polymer. Unless otherwise indicated, a particular polypeptide sequence also implicitly encompasses conservatively modified variants thereof.
The term "recombinant human antibody", as used herein, includes all human antibodies that are prepared, expressed, created or isolated by recombinant means, such as antibodies isolated from an animal (e.g., a mouse) that is transgenic or transchromosomal for human immunoglobulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell transformed to express the human antibody, e.g., from a transfectoma, antibodies isolated from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isolated by any other means that involve splicing of all or a portion of a human immunoglobulin gene, sequences to other DNA sequences. Such recombinant human antibodies have variable regions in which the framework and CDR regions are derived from human germline immunoglobulin sequences. In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis (or, when an animal transgenic for human Ig sequences is used, in vivo somatic mutagenesis) and thus the amino acid sequences of the VH and VL regions of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.
The term "recombinant host cell" (or simply "host cell") refers to a cell into which a recombinant expression vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term "host cell" as used herein. The term "subject" includes human and non-human animals. Non-human animals include all vertebrates (e.g.: mammals and non-mammals) such as, non-human primates (e.g.: cynomolgus monkey), sheep, dog, cow, chickens, amphibians, and reptiles. Except when noted, the terms "patient" or "subject" are used herein
interchangeably. As used herein, the terms "cyno" or "cynomolgus" refer to the cynomolgus monkey (Macaca fascicularis).
As used herein, the term "treating" or "treatment" of any disease or disorder (i.e., AMD) refers in one embodiment, to ameliorating the disease or disorder (i.e., slowing or arresting or reducing the development of the disease or at least one of the clinical symptoms thereof). In another embodiment "treating" or "treatment" refers to alleviating or ameliorating at least one physical parameter including those which may not be discernible by the patient. In yet another embodiment, "treating" or "treatment" refers to modulating the disease or disorder, either physically, (e.g., stabilization of a discernible symptom), physiologically, (e.g., stabilization of a physical parameter), or both. In yet another embodiment, "treating" or "treatment" refers to preventing or delaying the onset or development or progression of the disease or disorder. "Prevention" as it relates to AMD means any action that prevents or slows a worsening in visual function, retinal anatomy, and/or an AMD disease parameter, as described below, in a patient at rist for said worsening. More specifically, "treatment" of AMD means any action that results in the improvement or preservation of visual function and/or reginal anatomy. Methods for assessing treatment and/or prevention of disease are known in the art and described hereinbelow.
The term "vector" is intended to refer to a polynucleotide molecule capable of transporting another polynucleotide to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments may be ligated. Another type of vector is a viral vector, such as an adeno- associated viral vector (AAV, or AAV2), wherein additional DNA segments may be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as "recombinant expression vectors" (or simply, "expression vectors"). In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, "plasmid" and "vector" may be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 Depicts the Factor P binding site. Firgure 1A illustrates the relative position TSR5 domain of Factor P and the TSR5 sequence fragments: A, B, C and D. Figure 1 B shows the human TSR5 sequence aligned with the mouse sequence. Brackets indicate the sequence fragments of TSR5. Figure 1 C illustrates the antibodies bind to region B of TSR5.
Figure 2 shows the results of a hemolytic assay demonstrating inhibition of the alternative complement pathway in 20% human serum.
Figure 3 shows an isobologram generated using the data from the hemolytic assay depicted in Figure 2.
Figure 4 shows the % inhibition of macrophage infiltrates in a mouse poly-IC model, comparing the inhibition of anti-FactorP and anti-C5 antibodies singly and in
combination.
Figure 5 shows an isobologram generated using the data from the poly-IC results depicted in Figure 4.
DETAILED DESCRIPTION
The present invention is based, in part, on the discovery of antibody molecules that specifically bind to both human and cynomolgus Factor P. The invention relates to both full IgG format antibodies as well as antigen binding fragments thereof, such as Fab fragments (e.g., see antibodies NVS965-S, NVS962-S, NVS804 and NVS807 ).
Accordingly, the present invention provides antibodies that specifically bind to Factor P (e.g., human Factor P, cynomolgus Factor P, rat Factor P, rabbit Factor P), pharmaceutical compositions, production methods, and methods of use of such antibodies and compositions. Factor P Antibodies & Antigen Binding Fragments
The present invention provides antibodies that specifically bind to Factor P. In some embodiments, the present invention provides antibodies that specifically bind to human, cynomolgus, rat and/or rabbit Factor P. Antibodies of the invention include, but are not limited to, the human monoclonal antibodies and Fabs, isolated as described in the Examples.
The present invention provides antibodies that specifically bind a Factor P protein (e.g., human and/or cynmolgus Factor P), wherein the antibodies comprise a VH domain having an amino acid sequence of SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 or 273. The present invention also provides antibodies that specifically bind to a Factor P protein, wherein the antibodies comprise a VH CDR having an amino acid sequence of any one of the VH CDRs listed in Table 1 , infra. In particular, the invention provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), wherein the antibodies comprise (or alternatively, consist of) one, two, three, or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1 , infra.
The present invention provides antibodies that specifically bind to a Factor P protein, said antibodies comprising a VL domain having an amino acid sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274. The present invention also provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), said antibodies comprising a VL CDR having an amino acid sequence of any one of the VL CDRs listed in Table 1 , infra. In particular, the invention provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1 , infra.
Other antibodies of the invention include amino acids that have been mutated, yet have at least 60, 70, 80, 85, 90 or 95 percent identity in the CDR regions with the CDR regions depicted in the sequences described in Table 1. In some embodiments, it includes mutant amino acid sequences wherein no more than 1 , 2, 3, 4 or 5 amino acids have been mutated in the CDR regions when compared with the CDR regions depicted in the sequence described in Table 1 .
The present invention also provides nucleic acid sequences that encode VH, VL, the full length heavy chain, and the full length light chain of the antibodies that specifically bind to a Factor P protein {e.g., human and/or cynomolgus Factor P). Such nucleic acid sequences can be optimized for expression in mammalian cells (for example, Table 1 shows the optimized nucleic acid sequences for the heavy chain and light chain of antibodies of the invention).
Table 1 Examples of Factor P Antibodies, Fabs and Factor P Proteins
AMINO ACID SEQUENCE IDENTIFIER ( SEQ . ID . NO : ) AND SEQUENCE SEQUENCE OR POLYNUCLEOTIDE
(PN)
NVS962
CDRH1 1/281
SYAIS (Rabat)/ GGTFNSY (Chothia)
CDRH2 2/282
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 3/283
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 4/284
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 5/285
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 6/286
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 7
EVQLVQSGAEVRRPGSSVRVSCRASGGTFNSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 8
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 9
EVQLVQSGAEVRRPGSSVRVSCRASGGTFNSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 10
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 11
SEQ. ID. NO: 7 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 12
SEQ. ID. NO: 8 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 13
SEQ. ID. NO: 9 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 14
SEQ. ID. NO: 10 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS963
CDRH1 15/287
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 16/288
PINPYYGDAIYAQKFQG (Rabat) / NPYYGD (Chothia)
CDRH3 17/289
YYSDYMDY (Rabat) / YYSDYMDY (Chothia)
CDRL1 18/290
TGSSSNIGAGYDVH (Rabat)/ SSSNIGAGYD (Chothia)
CDRL2 19/291
DNSHRPS (Rabat)/ DNS (Chothia)
CDRL3 20/292
ASYDESAHS (Rabat)/ YDESAHS (Chothia)
VH 21
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GPINPYYGDAIYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARYYSDYMDYWGQGTLVTVSS
VL 22
QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKL LIHDNSHRPSGVPDRFSGSKSGTSASLAITGLQSEDEADYYCASYDES AHSVFGGGTKLTVL
HEAVY CHAIN 23
EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GPINPYYGDAIYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARYYSDYMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC
LIGHT CHAIN 24
QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKL LIHDNSHRPSGVPDRFSGSKSGTSASLAITGLQSEDEADYYCASYDES AHSVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFY PGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSH RSYSCQVTHEGSTVEKTVAPTECS
PN ENCODING 25
SEQ.ID.NO:21 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTTAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCCCCATCAACCCCTACTACGGCGACGCCATCTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCCGGTACTACAGCGACTACATGGACTACTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 26
SEQ.ID.NO:22 CAGTCAGTGCTGACCCAGCCTCCCTCTGTGTCTGGCGCCCCTGGCCAG
AGAGTGACCATCAGCTGCACCGGCTCCAGCAGCAACATCGGAGCTGGA TACGACGTGCACTGGTATCAGCAGCTGCCCGGCACAGCCCCTAAGCTG CTGATCCACGACAACAGCCACAGACCCAGCGGCGTGCCCGATAGATTC AGCGGCAGCAAGAGCGGCACCAGCGCCAGCCTGGCCATCACCGGCCTG CAGAGCGAGGACGAGGCCGACTACTACTGCGCCAGCTACGACGAGAGC GCCCACAGCGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 27
SEQ.ID.NO:23 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTTAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCCCCATCAACCCCTACTACGGCGACGCCATCTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCCGGTACTACAGCGACTACATGGACTACTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 28
SEQ.ID.NO:24 CAGTCAGTGCTGACCCAGCCTCCCTCTGTGTCTGGCGCCCCTGGCCAG
AGAGTGACCATCAGCTGCACCGGCTCCAGCAGCAACATCGGAGCTGGA TACGACGTGCACTGGTATCAGCAGCTGCCCGGCACAGCCCCTAAGCTG CTGATCCACGACAACAGCCACAGACCCAGCGGCGTGCCCGATAGATTC AGCGGCAGCAAGAGCGGCACCAGCGCCAGCCTGGCCATCACCGGCCTG CAGAGCGAGGACGAGGCCGACTACTACTGCGCCAGCTACGACGAGAGC GCCCACAGCGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAG CCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG CTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTAC CCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAG GCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTAC GCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC AGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAG ACAGTGGCCCCTACAGAATGTTCA
NVS964
CDRH1 29/293
SHYMH (Rabat)/ GYTFTSH (Chothia)
CDRH2 30/294
KINADLGDTNYAQKFQG (Rabat) / NADLGD (Chothia)
CDRH3 31/295
DGIEHGGHYYWGYLFDI (Rabat) / DGIEHGGHYYWGYLFDI (Chothia)
CDRL1 32/296
SGDS IREYYVH (Rabat)/ DSIREYY (Chothia)
CDRL2 33/297
DDTNRPS (Rabat)/ DDT (Chothia)
CDRL3 34/298
AAWDFSPAI (Rabat)/ WDFSPAI (Chothia)
VH 35
EVQLVQSGAEVRRPGASVRVSCRASGYTFTSHYMHWVRQAPGQGLEWM GRINADLGDTNYAQRFQGRVTMTRDTS ISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSS
VL 36
SYELTQPPSVSVAPGQTARISCSGDSIREYYVHWYQQRPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCAAWDFSPAI VFGGGTRLTVL
HEAVY CHAIN 37
EVQLVQSGAEVRRPGASVRVSCRASGYTFTSHYMHWVRQAPGQGLEWM GRINADLGDTNYAQRFQGRVTMTRDTS ISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSSASTRGPSVFPLAPSSRST SGGTAALGCLVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SVVTVPSSSLGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 38
SYELTQPPSVSVAPGQTARISCSGDSIREYYVHWYQQRPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCAAWDFSPAI VFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLI SDFYPGA VTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRSY SCQVTHEGSTVERTVAPTECS
PN ENCODING 39
SEQ.ID.NO:35 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAAGATCAACGCCGACCTGGGCGACACCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCA
PN ENCODING 40
SEQ.ID.NO:36 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 41
SEQ.ID.NO:37 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAAGATCAACGCCGACCTGGGCGACACCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCAGCATCC ACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACC TCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTG CACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGC AGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATC TGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGT
PN ENCODING 42
SEQ.ID.NO:38 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAGGCT GCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCC AACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCC GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG GAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC AGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTAC AGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCC CCTACAGAATGTTCA
NVS966
CDRH1 43/299
NYWIG (Rabat)/ GYSFTNY (Chothia)
CDRH2 44/300
RIDPGESLTNYAPSFQG (Rabat) / DPGESL (Chothia)
CDRH3 45/301
TGVADVDMPFAH (Rabat) / TGVADVDMPFAH (Chothia)
CDRL1 46/302
SGDNLGSYYVN (Rabat)/ DNLGSYY (Chothia)
CDRL2 47/303
GDSERPS (Rabat)/ GDS (Chothia)
CDRL3 48/304
GSWDITSF (Rabat) / WDITSF (Chothia)
VH 49
EVQLVQSGAEVRRPGESLRISCRGSGYSFTNYWIGWVRQMPGRGLEWM GRIDPGESLTNYAPSFQGQVTISADRS ISTAYLQWSSLRASDTAMYYC ARTGVADVDMPFAHWGQGTLVTVSS
VL 50
SYVLTQPPSVSVAPGRTARISCSGDNLGSYYVNWYQQRPGQAPVLVIY GDSERPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCGSWDITSFV FGGGTKLTVL
HEAVY CHAIN 51
EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYWIGWVRQMPGKGLEWM GRIDPGESLTNYAPSFQGQVTISADKS ISTAYLQWSSLKASDTAMYYC ARTGVADVDMPFAHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTA ALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTV PSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC
LIGHT CHAIN 52
SYVLTQPPSVSVAPGKTARISCSGDNLGSYYVNWYQQKPGQAPVLVIY GDSERPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCGSWDITSFV FGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAV TVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYS CQVTHEGSTVEKTVAPTECS
PN ENCODING 53
SEQ. ID. NO: 49 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAGCCTGGCGAG
AGCCTGAAGATCAGCTGCAAGGGCAGCGGCTACAGCTTCACCAACTAC TGGATCGGCTGGGTGCGCCAGATGCCTGGCAAGGGCCTGGAATGGATG GGCAGAATCGACCCCGGCGAGTCCCTGACCAACTACGCCCCCAGCTTC CAGGGCCAGGTGACAATCAGCGCCGACAAGAGCATCAGCACCGCCTAT CTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC GCCAGAACCGGCGTGGCCGACGTGGACATGCCTTTTGCCCACTGGGGC CAGGGCACCCTGGTGACCGTGAGCTCA
PN ENCODING 54
SEQ. ID. NO: 50 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCTACTACGTG AACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC GGCGACAGCGAGAGGCCTAGCGGCATCCCCGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGGCTCCTGGGACATCACCAGCTTCGTG TTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 55
SEQ. ID. NO: 51 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAGCCTGGCGAG
AGCCTGAAGATCAGCTGCAAGGGCAGCGGCTACAGCTTCACCAACTAC TGGATCGGCTGGGTGCGCCAGATGCCTGGCAAGGGCCTGGAATGGATG GGCAGAATCGACCCCGGCGAGTCCCTGACCAACTACGCCCCCAGCTTC CAGGGCCAGGTGACAATCAGCGCCGACAAGAGCATCAGCACCGCCTAT CTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC GCCAGAACCGGCGTGGCCGACGTGGACATGCCTTTTGCCCACTGGGGC CAGGGCACCCTGGTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCG GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCG GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTG TCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCT GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTG CCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCAC AAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 56
SEQ.ID.NO:52 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCTACTACGTG AACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC GGCGACAGCGAGAGGCCTAGCGGCATCCCCGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGGCTCCTGGGACATCACCAGCTTCGTG TTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCC CCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAAC AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTG ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAG ACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGC TATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGC TGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCT ACAGAATGTTCA
NVS965
CDRH1 57/305
SYAIS (Rabat)/ GGTFNSY (Chothia)
CDRH2 58/306
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 59/307
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 60/308
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 61/309
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 62/310
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 63
EVQLVQSGAEVRRPGSSVRVSCRASGGTFNSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 64
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVL
HEAVY CHAIN 65
EVQLVQSGAEVRRPGSSVRVSCRASGGTFNSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 66
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 67
SEQ. ID. NO: 63 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 68
SEQ. ID. NO: 64 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 69
SEQ. ID. NO: 65 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 70
SEQ. ID. NO: 66 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS967
CDRH1 71/311
SHYMH (Rabat)/ GYTFTSH (Chothia)
CDRH2 72/312
NINPVDGGTEYAQKFQG (Rabat) / NPVDGG (Chothia)
CDRH3 73/313
DGIEHGGHYYWGYLFDI (Rabat) / DGIEHGGHYYWGYLFDI (Chothia)
CDRL1 74/314
SGDS IREYYVH (Rabat)/ DSIREYY (Chothia)
CDRL2 75/315
DDTNRPS (Rabat)/ DDT (Chothia)
CDRL3 76/316
AAWDFSPAI (Rabat)/ WDFSPAI (Chothia)
VH 77
EVQLVQSGAEVRRPGASVRVSCRASGYTFTSHYMHWVRQAPGQGLEWM GNINPVDGGTEYAQRFQGRVTMTRDTS ISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSS
VL 78
SYVLTQPPSVSVAPGRTARISCSGDSIREYYVHWYQQRPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCAAWDFSPAI VFGGGTRLTVL
HEAVY CHAIN 79 EVQLVQSGAEVKKPGASVKVSCKASGYTFTSHYMHWVRQAPGQGLEWM GNINPVDGGTEYAQKFQGRVTMTRDTS ISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSSASTKGPSVFPLAPSSKST SGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC
LIGHT CHAIN 80
SYVLTQPPSVSVAPGKTARISCSGDSIREYYVHWYQQKPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCAAWDFSPAI VFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLI SDFYPGA VTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSY SCQVTHEGSTVEKTVAPTECS
PN ENCODING 81
SEQ. ID. NO: 77 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAACATCAACCCCGTGGACGGCGGCACCGAGTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCA
PN ENCODING 82
SEQ. ID. NO: 78 TCTTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 83
SEQ. ID. NO: 79 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAACATCAACCCCGTGGACGGCGGCACCGAGTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCAGCCTCC ACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACC TCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTG CACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGC AGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATC TGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGT
PN ENCODING 84
SEQ. ID. NO: 80 TCTTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAGGCT GCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCC AACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCC GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG GAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC AGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTAC AGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCC CCTACAGAATGTTCA
NVS807
CDRH1 85/317
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 86/318
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 87/319
HGGYSYFDS (Rabat)/ HGGYYFDS (Chothia)
CDRL1 88/320
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 89/321
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 90/322
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 91
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSS
VL 92
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 93
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 94
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 95
SEQ. ID.NO: 91 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 96
SEQ. ID.NO: 92 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 97
SEQ. ID.NO: 93 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 98
SEQ. ID. NO: 94 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS808
CDRH1 99/323
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 100/324
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 101/325
HGGYIFDS (Rabat) / HGGYIFDS (Chothia)
CDRL1 102/326
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 103/327
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 104/328
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 105
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSS
VL 106
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 107
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC LIGHT CHAIN 108
SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS
PN ENCODING 109
SEQ. ID. NO: 105 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 110
SEQ. ID. NO: 106 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 111
SEQ. ID. NO: 107 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 112
SEQ. ID. NO: 108 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS806
CDRH1 113/329
SYAIS (Rabat) / GGTFSSY (Chothia)
CDRH2 114/330
RIIPIFGTANYAQKFQG (Rabat) / IPIFGT (Chothia)
CDRH3 115/331
HGGYVFDS (Rabat) / HGGYVFDS (Chothia)
CDRL1 116/332
SGDNLGSRYVD (Rabat) / DNLGSRY (Chothia)
CDRL2 117/333
SDNNRPS (Rabat) / SDN (Chothia)
CDRL3 118/334
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 119
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSS
VL 120
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 121
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 122
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 123
SEQ. ID.NO: 119 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 124
SEQ. ID.NO: 120 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 125
SEQ. ID.NO: 121 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 126
SEQ. ID. NO: 122 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS804
CDRH1 127/335
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 128/336
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 129/337
HGGYVFDS (Rabat) / HGGYIFDS (Chothia)
CDRL1 130/338
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 131/339
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 132/340
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 133
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSS
VL 134
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVL
HEAVY CHAIN 135
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC
LIGHT CHAIN 136
SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS
PN ENCODING 137
SEQ. ID.NO: 133 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 138
SEQ. ID.NO: 134 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 139
SEQ. ID.NO: 135 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 140
SEQ. ID.NO: 136 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS809 CDRH1 141/341
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 142/342
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 143/343
HGGYYFDS (Rabat) / HGGYYFDS (Chothia)
CDRL1 144/344
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 145/345
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 146/346
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 147
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSS
VL 148
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVL
HEAVY CHAIN 149
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 150
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 151
SEQ. ID. NO: 147 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 152
SEQ. ID. NO: 148 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 153
SEQ. ID. NO: 149 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 154
SEQ. ID. NO: 150 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS805
CDRH1 155/347
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 156/348
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 157/349
HGGYVFDS (Rabat) / HGGYVFDS (Chothia)
CDRL1 158/350
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 159/351
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 160/352
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 161
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSS
VL 162
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVL
HEAVY CHAIN 163
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 164
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS
PN ENCODING 165
SEQ. ID. NO: 161 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 166
SEQ. ID. NO: 162 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 167
SEQ. ID. NO: 163 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 168
SEQ. ID. NO: 164 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS962-S
CDRH1 169/353
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 170/354
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 171/355
HGGYSFDS (Rabat) / HGGYSFDS (Chothia) CDRL1 172/356
SGDNLGSKYVD (Rabat)/ DNLGSKY (Chothia)
CDRL2 173/357
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 174/358
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 175
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 176
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 177
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 178
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 179
SEQ. ID. NO: 175 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 180
SEQ. ID. NO: 176 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 181
SEQ. ID. NO: 177 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 182
SEQ. ID. NO: 178 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS962-Q
CDRH1 183/359
SYAIS (Rabat)/ GGTFQSY (Chothia)
CDRH2 184/360
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 185/361
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 186/362
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 187/363
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 188/364
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 189
EVQLVQSGAEVRRPGSSVRVSCRASGGTFQSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 190
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 191
EVQLVQSGAEVRRPGSSVRVSCRASGGTFQSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 192
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 193 SEQ. ID.NO: 189 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 194
SEQ. ID.NO: 190 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 195
SEQ. ID.NO: 191 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 196
SEQ. ID.NO: 192 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS962-S31A
CDRH1 197/365
SYAIS (Rabat)/ GGTFNAY (Chothia)
CDRH2 198/366
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 199/367
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 200/368
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia) CDRL2 201/369
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 202/370
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 203
EVQLVQSGAEVRRPGSSVRVSCRASGGTFNAYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 204
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 205
EVQLVQSGAEVRRPGSSVRVSCRASGGTFNAYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 206
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 207
SEQ.ID.NO:203 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACGCCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 208
SEQ.ID.NO:204 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 209
SEQ.ID.NO:205 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACGCCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 210
SEQ.ID.NO:206 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS962-G
CDRH1 211/371
SYAIS (Rabat)/ GGTFGSY (Chothia)
CDRH2 212/372
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 213/373
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 214/374
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 215/375
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 216/376
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 217
EVQLVQSGAEVRRPGSSVRVSCRASGGTFGSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 218
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVL
HEAVY CHAIN 219
EVQLVQSGAEVRRPGSSVRVSCRASGGTFGSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 220
SYELTQPPSVSVAPGQTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 221
SEQ.ID.NO:217 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCGGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 222
SEQ.ID.NO:218 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 223
SEQ.ID.NO:219 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCGGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 224
SEQ.ID.NO:220 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS962-T
CDRH1 225/377
SYAIS (Rabat)/ GGTFTSY (Chothia)
CDRH2 226/378
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 227/379
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 228/380
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 229/381
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 230/382
QTYTSGNNYL (Rabat) / YTSGNNYL (Chothia)
VH 231 EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 232
SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL
HEAVY CHAIN 233
EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC
LIGHT CHAIN 234
SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS
PN ENCODING 235
SEQ.ID.NO:231 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 236
SEQ.ID.NO:232 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 237
SEQ.ID.NO:233 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 238
SEQ.ID.NO:234 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG
GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC
CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG
GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA
GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA
GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC
AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC
TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG
GCCCCTACAGAATGTTCA
NVS965 -T
CDRH1 239/383
SYAIS (Rabat)/ GGTFTSY (Chothia)
CDRH2 240/384
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 241/385
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 242/386
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 243/387
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 244/388
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 245
EVQLVQSGAEVRRPGSSVRVSCRASGGTFTSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 246
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVL
HEAVY CHAIN 247
EVQLVQSGAEVRRPGSSVRVSCRASGGTFTSYAISWVRQAPGQGLEWM
GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC
ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC
LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS
LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 248
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY
SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT
EVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG
AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS
YSCQVTHEGSTVERTVAPTECS
PN ENCODING 249
SEQ. ID .NO 245 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 250
SEQ. ID .NO 246 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 251
SEQ.ID.NO:247 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 252
SEQ.ID.NO:248 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS965-Q
CDRH1 253/389
SYAIS (Rabat)/ GGTFQSY (Chothia)
CDRH2 254/390
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 255/391
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 256/392
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 257/393
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 258/394
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 259
EVQLVQSGAEVRRPGSSVRVSCRASGGTFQSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 260
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVL
HEAVY CHAIN 261
EVQLVQSGAEVKKPGSSVKVSCKASGGTFQSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC
LIGHT CHAIN 262
SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS
PN ENCODING 263
SEQ.ID.NO:259 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 264
SEQ.ID.NO:260 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 265
SEQ.ID.NO:261 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 266
SEQ.ID.NO:262 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
NVS965-S
CDRH1 267/395
SYAIS (Rabat)/ GGTFSSY (Chothia)
CDRH2 268/396
RIIPIFGTANYAQKFQG (Rabat)/ IPIFGT (Chothia)
CDRH3 269/397
HGGYSFDS (Rabat) / HGGYSFDS (Chothia)
CDRL1 270/398
SGDNLGSRYVD (Rabat)/ DNLGSRY (Chothia)
CDRL2 271/399
SDNNRPS (Rabat)/ SDN (Chothia)
CDRL3 272/400
ATYDSSPRTE (Rabat) / YDSSPRTE (Chothia)
VH 273
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS
VL 274
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVL
HEAVY CHAIN 275
EVQLVQSGAEVRRPGSSVRVSCRASGGTFSSYAISWVRQAPGQGLEWM GRI I PI FGTANYAQRFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTRGPSVFPLAPSSRSTSGGTAALGC LVRDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHRPSNTRVDRRVEPRSC
LIGHT CHAIN 276
SYVLTQPPSVSVAPGRTARISCSGDNLGSRYVDWYQQRPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTRLTVLGQPRAAPSVTLFPPSSEELQANRATLVCLISDFYPG AVTVAWRADSSPVRAGVETTTPSRQSNNRYAASSYLSLTPEQWRSHRS YSCQVTHEGSTVERTVAPTECS
PN ENCODING 277
SEQ.ID.NO:273 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC
AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA
PN ENCODING 278
SEQ.ID.NO:274 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA
PN ENCODING 279 SEQ.ID.NO:275 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT
PN ENCODING 280
SEQ.ID.NO:276 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG
ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA
Human Factor P 401
NP 001138724.1 PVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGGLCQPCR
SPRWSLWSTWAPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTLEWQL QACEDQQCCPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAPKCGG HCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETR SRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSP CPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDG EWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQ QDIRHCYS IQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLP KYPPTVSMVEGQGEKNVTFWGRPLPRCEELQGQKLWEEKRPCLHVPA CKDPEEEEL
Chimpanzee 402
Factor P MITEGAQAPCLLLPPLLLLLTLPATGSDPVLCFTQYEESSGKCKGLLG
XP 001136665.1 GGVSVKDCCLNTAYAYQERNGGLCQPCRSPRWSLWSTWAPCSVTCSEG
SQLRYRRCVGWNGQCSERVALGTLEWQLQACEDKQCCPEMGGWSDWGP WEPCSVTCSKGMRTRRRACNHPAPKCGGHCPGEAQESEACDTQQVCPT HGAWAAWGPWSPCSGSCHGGPHEPKETRSRTCSAPEPSQKPPGKPCPG PAYEHRKCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTIERRTCNRPV PQHGGPSCAGDATRTHICNTAAPCPVDGEWDLWGQWSTCVRRNMKSIS CEEIPGQQSRWRTCKGRKFDGHRCTGQQQDIRHCYSIQHCPLKGSWSE WSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFW GRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEKL
Rat Factor P 403
NP 001100227.1 MPVGMQAPQWLLLLLLILPTTGSDPVLCFTQYEEPSGRCKGLLGRDIR
VEDCCLNTAYAFQEHDGGLCQSCRSPQWSAWSSWGPCSVTCSEGSQLR HRRCVGRGGQCSEKAAPGTLEWQLQACEDQLCCPEMGGWSEWGPWGPC SVTCSKGTQTRQRLCDNPAPKCGGHCPGEAQQSQACDTQKICPTHGAW ASWGPWSACSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGTAYE HRGCSGLPPCPVAGGWGPWGPSSPCPVTCGLGQTLERRTCDHPVPRHG GPFCAGDATRKHVCNTAMPCPVNGEWEAWGKWSHCSRVRMKSI SCDEI PGQQSRSRSCGGRKFDGQPCTGKLQDIRHCYDIHNCVLKGSWSQWSTW GLCTPPCGPNPTRVRQRLCTPLLPKYSPTVSMVEGQGEKNVTFWGIPR PLCEVLQGQKLVVEEKRPCLHVPSCRDPEEKKP
Rabbit Factor P 404
XP 002719931.1 MPAQAQPPLPLLLLPLLLTLPATGADPWCFTEYDEPSGKCKGLLGGG
VSVEHCCLNAAYAFQEPGSGLCHACRSPLWSPWSAWAPCSVTCSEGSQ LRHRRCVGQGGPCSEKAAPGTLQWQLQACEDQPCCPEIGGWSDWGPWR PCSVTCSKGTKTRQRACDRPAPKCGGRCPGEAQESEACDTKQVCPTHG LWAAWGPWSPCSGSCHGGPQVPKETRSRTCSAPEPSKQPPGKPCSGPA YEEQSCAGLPPCPVAGGWGPWGPVSSCSVTCGLGKTLEKRTCDHPVPQ HGGPFCTGDATRTHICNTAVPCPVNGEWEAWGEWSECSRPGRKSISCE EVPGQQRRTRVCKGRKFDGQRCAGEYQDIRHCYNIQRCRLKGSWLEWS SWGLCTPPCGPSPTRTRQRLCTALLPKFPPTISLVEGQGEKNVTFWGK PWPQCEQLQGQKLVVEEKRPCLHVPACKDPEEKP
Mouse Factor P 405
NP 032849.2 MPAEMQAPQWLLLLLVILPATGSDPVLCFTQYEESSGRCKGLLGRDIR
VEDCCLNAAYAFQEHDGGLCQACRSPQWSAWSLWGPCSVTCSEGSQLR HRRCVGRGGQCSENVAPGTLEWQLQACEDQPCCPEMGGWSEWGPWGPC SVTCSKGTQIRQRVCDNPAPKCGGHCPGEAQQSQACDTQKTCPTHGAW ASWGPWSPCSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGPAYE HKACSGLPPCPVAGGWGPWSPLSPCSVTCGLGQTLEQRTCDHPAPRHG GPFCAGDATRNQMCNKAVPCPVNGEWEAWGKWSDCSRLRMS INCEGTP GQQSRSRSCGGRKFNGKPCAGKLQDIRHCYNIHNCIMKGSWSQWSTWS LCTPPCSPNATRVRQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGTPRP LCEALQGQKLWEEKRSCLHVPVCKDPEEKKP
TSR5 Domain of 406
SEQ ID NO: 401 VDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQD
IRHCYSIQHCP
Region B of TSR 407
5 domain PCIRRNMKSISCQEIPGQQSRGR
Region of TSR 5 408
binding domain KSISC
TSR5 Domain of 409
mouse SEQ ID VNGEWEAWGKWSDCSRLRMSINCEGTPGQQSRSRSCGGRKFNGKPCAGKLQDI NO: 405 RHCY IHNCI
Table 2 Examples of C5 Antibodies, Fabs and C5 Proteins
Antibody 8109 Sequence Identifier (SEQ ID NO:)
CDRH1 410
SYAIS
CDRH2 41 1
GIGPFFGTANYAQKFQG
CDRH3 412
DTPYFDY
CDRL1 413
SGDSIPNYYVY
CDRL2 414
DDSNRPS
CDRL3 415
QSFDSSLNAEV VH 416
EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQG RVTITADESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSS
VL 417
SYELTQPLSVSVALGQTARITCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGN TATLTISRAQAGDEADYYCQSFDSSLNAEVFGGGTKLTVL
Heavy chain 418
EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQG
RVTITADESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKST
SGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVN
HKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDP
EVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA
KGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL
YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Light chain 419
SYELTQPLSVSVALGQTARITCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGSNSGN TATLTISRAQAGDEADYYCQSFDSSLNAEVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVC LISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGST VEKTVAPTECS
PN encoding 420
SEQ ID GAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCT NO:416 GCAAAGCCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAG
GGTCTCGAGTGGATGGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCA
GGGCCGGGTGACCATTACCGCGGATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTG
CGTAGCGAAGATACGGCCGTGTATTATTGCGCGCGTGATACTCCTTA I I I I GATTATTGGGGCCA
AGGCACCCTGGTGACGGTTAGCTCA
PN encoding 421
SEQ ID TCCTATGAACTCACACAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCCGGATCACCT NO:417 GCTCCGGCGACAGCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCC
CGTGCTGGTGATCTACGACGACAGCAACCGGCCCAGCGGCATCCCCGAGCGGTTCAGCGGCAG
CAACAGCGGCAACACCGCCACCCTGACCATTTCCAGAGCACAGGCAGGCGACGAGGCCGACTA
CTACTGCCAGAGCTTCGACAGCAGCCTGAACGCCGAGGTGTTCGGCGGAGGGACCAAGTTAACC
GTCCTA
PN encoding 422
SEQ ID GAGGTGCAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCT NQ:418 GCAAAGCCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAG
GGTCTCGAGTGGATGGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCA
GGGCCGGGTGACCATTACCGCGGATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTG
CGTAGCGAAGATACGGCCGTGTATTATTGCGCGCGTGATACTCCTTA I I I I GATTATTGGGGCCA
AGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCC
TCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCG
AACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTG
TCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGG
CACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT
GAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGG
GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAG
GTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGG
ACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC
GGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA
GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC
TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA
GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTAC
AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC
ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
PN encoding 423
SEQ ID TCCTATGAACTCACACAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCCGGATCACCT NO:419 GCTCCGGCGACAGCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCC
CGTGCTGGTGATCTACGACGACAGCAACCGGCCCAGCGGCATCCCCGAGCGGTTCAGCGGCAG
CAACAGCGGCAACACCGCCACCCTGACCATTTCCAGAGCACAGGCAGGCGACGAGGCCGACTA
CTACTGCCAGAGCTTCGACAGCAGCCTGAACGCCGAGGTGTTCGGCGGAGGGACCAAGTTAACC
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTC
AAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC
CTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAG
CAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGA
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAAT
GTTCA
Optimized PN 424
encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAGAAGCCCGGTAGCAGCGTCAAGGTGTCC ID NO:418 TGCAAGGCCAGCGGCGGCACCTTCAGCAGCTACGCCATCAGCTGGGTGCGGCAGGCCCCAGGC
CAGGGCCTGGAGTGGATGGGCGGCATCGGCCCATTCTTCGGCACCGCCAACTACGCCCAGAAG
TTCCAGGGCAGGGTCACCATCACCGCCGACGAGAGCACCAGCACCGCCTACATGGAGCTGTCCA
GCCTGAGAAGCGAGGACACCGCCGTGTACTACTGCGCCAGAGACACCCCCTACTTCGACTACTG
GGGCCAGGGCACCCTGGTGACCGTGAGCAGCGCTAGCACCAAGGGCCCCAGCGTGTTCCCCCT
GGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGACTA
CTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTT
CCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAG
CAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGAC
AAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAA
GCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCA
GGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCA
ACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTACA
ACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGA
ATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGCC
AAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG
AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGG
AGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCA
GCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAG
CTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCC
GGCAAG
Optimized PN 425
encoding SEQ AGCTACGAGCTGACCCAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCAGGATCACC ID NO:419 TGCAGCGGCGACAGCATCCCCAACTACTACGTGTACTGGTATCAGCAGAAGCCCGGCCAGGCCC
CCGTGCTGGTGATCTACGACGACAGCAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCA
GCAACAGCGGCAACACCGCCACCCTGACCATCAGCAGAGCCCAGGCCGGCGACGAGGCCGACT
ACTACTGCCAGAGCTTCGACAGCTCACTGAACGCCGAGGTGTTCGGCGGAGGGACCAAGCTGAC
CGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCT
GCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTG
GCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAG
AGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACA GGTCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCG AGTGCAGC
Antibody 8110 Sequence Identifier (SEQ ID NO:) and Sequence or comments
CDRH1 426
NYIS
CDRH2 427
IIDPDDSYTEYSPSFQG
CDRH3 428
YEYGGFDI
CDRL1 429
SGDNIGNSYVH
CDRL2 430
KDNDRPS
CDRL3 431
GTYDIESYV
VH 432
EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQV TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
VL 433
SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCGTYDIESYVFGGGTKLTVL
Heavy chain 434
EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQV
TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTS
GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNH
KPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPE
VKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK
GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY
SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Light chain 435
SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCGTYDIESYVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLIS DFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVE KTVAPTECS
PN encoding 436
SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:432 GCAAAGGTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGT
CTCGAGTGGATGGGCATTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTC I I I I CAGGGT CAGGTCACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGC GAGCGATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGG I I I I GATATTTGGGGCCAAG GCACCCTGGTGACGGTTAGCTCA
PN encoding 437
SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:434 GTAGCGGCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCA
GTTCTTGTGATTTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC
AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT
GCGGTACTTATGATATTGAGTCTTATGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTA
PN encoding 438
SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:435 GCAAAGGTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGT
CTCGAGTGGATGGGCATTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTC 1 1 1 1 CAGGGT
CAGGTCACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGC
GAGCGATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGG 1 1 1 1 GATATTTGGGGCCAAG
GCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTC
CTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAA
CCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTC
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCA
CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGA
GCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGA
CCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGT
CACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGAC
GGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGG
GTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGG
TCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCG
AGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTG
ACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGC
CGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAG
CAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCAT
GAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
PN encoding 439
SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:436 GTAGCGGCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCA
GTTCTTGTGATTTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC
AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT
GCGGTACTTATGATATTGAGTCTTATGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAG
CCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG
CCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGA
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTAC
GCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCC
AGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
Optimized PN 440
encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGC ID NO:434 TGCAAGGGCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAG
GGCCTGGAGTGGATGGGCATCATCGACCCCGACGACAGCTACACCGAGTACAGCCCCAGCTTCC
AGGGCCAGGTGACCATCAGCGCCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCC
TGAAGGCCAGCGACACCGCCATGTACTACTGCGCCAGATACGAGTACGGCGGCTTCGACATCTG
GGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCCCCT
GGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGACTA
CTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTT
CCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAG
CAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGAC
AAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAA
GCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCA
GGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCA
ACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTACA
ACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGA
ATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGCC
AAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG
AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGG AGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCA GCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAG CTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCC GGCAAG
Optimized PN 441
encoding SEQ AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGC ID NO:435 TGCAGCGGCGACAACATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCC
CCCGTGCTGGTGATCTACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGC
AGCAACTCCGGCAACACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAGGACGAGGCCGAC
TACTACTGCGGCACCTACGACATCGAGTCATACGTGTTCGGCGGAGGGACCAAGCTGACCGTGC
TGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCTGCAGG
CCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTGGCCTG
GAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAGAGCAA
CAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTC
CTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTG
CAGC
Antibody 8111 Sequence Identifier (SEQ ID NO:) and Sequence or comments
CDRH1 442
TSGGGVS
CDRH2 443
NIDDADIKDYSPSLKS
CDRH3 444
GPYGFDS
CDRL1 445
TGTSS D I GTYN YVS
CDRL2 446
DDSNRPS
CDRL3 447
QSYDSQSIV
VH 448
EVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLANIDDADIKDYSPSLKSRL TISKDTSKNQWLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSS
VL 449
ESALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQHPGKAPKLMIYDDSNRPSGVSNRFSGSK SGNTASLTISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVL
Heavy chain 450
EVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLANIDDADIKDYSPSLKSRL
TISKDTSKNQWLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTS
GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNH
KPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPE
VKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK
GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY
SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Light chain 451
ESALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQHPGKAPKLMIYDDSNRPSGVSNRFSGSK SGNTASLTISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLV CLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGS TVEKTVAPTECS
PN encoding 452
SEQ ID GAGGTGACATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCT N 0:448 GTACCTTTTCCGGATTTAGCCTGTCTACTTCTGGTGGTGGTGTGTCTTGGATTCGCCAGCCGCCT GGGAAAGCCCTCGAGTGGCTGGCTAATATTGATGATGCTGATATTAAGGATTATTCTCCTTCTCTT AAGTCTCGTCTGACCATTAGCAAAGATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATG GACCCGGTGGATACGGCCACCTATTATTGCGCGCGTGGTCCTTATGG I I I I GATTCTTGGGGCCA AGGCACCCTGGTGACGGTTAGCTCA
PN encoding 453
SEQ ID GAAAGCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGT
N 0:449 GTACGGGTACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGG
AAGGCGCCGAAACTTATGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCG I I I I AGC
GGATCCAAAAGCGGCAACACCGCGAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCG
GATTATTATTGCCAGTCTTATGATTCTCAGTCTATTGTGTTTGGCGGCGGCACGAAGTTAACCGTC
CTA
PN encoding 454
SEQ ID GAGGTGACATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCT NO:450 GTACC I I I I CCGGATTTAGCCTGTCTACTTCTGGTGGTGGTGTGTCTTGGATTCGCCAGCCGCCT
GGGAAAGCCCTCGAGTGGCTGGCTAATATTGATGATGCTGATATTAAGGATTATTCTCCTTCTCTT
AAGTCTCGTCTGACCATTAGCAAAGATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATG
GACCCGGTGGATACGGCCACCTATTATTGCGCGCGTGGTCCTTATGG I I I I GATTCTTGGGGCCA
AGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCC
TCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCG
AACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTG
TCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGG
CACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT
GAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGG
GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAG
GTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGG
ACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC
GGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA
GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC
CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC
TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA
GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTAC
AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC
ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
PN encoding 455
SEQ ID GAAAGCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGT NO:451 GTACGGGTACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGG
AAGGCGCCGAAACTTATGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCG I I I I AGC
GGATCCAAAAGCGGCAACACCGCGAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCG
GATTATTATTGCCAGTCTTATGATTCTCAGTCTATTGTGTTTGGCGGCGGCACGAAGTTAACCGTC
CTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG
CCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG
GAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAAC
AACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCT
ACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTC
A
Optimized PN 456
encoding SEQ GAGGTGACCCTGAAGGAGAGCGGCCCAGCCCTGGTGAAGCCCACCCAGACCCTGACCCTGACT ID NO:448 TGCACCTTCAGCGGCTTCAGCCTGAGCACCAGCGGAGGGGGCGTGAGCTGGATCAGGCAGCCC
CCAGGTAAGGCCCTGGAGTGGCTGGCCAATATCGACGACGCCGATATCAAGGACTACAGCCCCA GCCTGAAGAGCAGGCTGACCATCAGCAAGGACACCAGCAAGAACCAGGTGGTGCTGACCATGAC
CAATATGGACCCCGTGGACACCGCCACCTACTACTGCGCCAGAGGCCCCTACGGCTTCGACAGC
TGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCCC
CTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGAC
TACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCT
TCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCA
GCAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGA
CAAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGA
AGCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGC
AGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTC
AACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTAC
AACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAG
AATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGC
CAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAA
GAACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGG
GAGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGC
AGCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCA
GCTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACC
CGGCAAG
Optimized PN 457
encoding SEQ GAGAGCGCCCTGACCCAGCCCGCCAGCGTGAGCGGCAGCCCAGGCCAGTCTATCACAATCAGC ID NO:449 TGCACCGGCACCTCCAGCGATATCGGCACCTACAACTACGTGAGCTGGTATCAGCAGCACCCCG
GCAAGGCCCCCAAGCTGATGATCTACGACGACAGCAACAGGCCCAGCGGCGTGAGCAACAGGTT
CAGCGGCAGCAAGAGCGGCAACACCGCCAGCCTGACAATCAGCGGCCTGCAGGCCGAGGACGA
GGCCGACTACTACTGCCAGAGCTACGACAGCCAGTCAATCGTGTTCGGCGGAGGGACCAAGCTG
ACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAG
CTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCG
TGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGC
AGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCA
CAGGTCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAAC
CGAGTGCAGC
Antibody 8113 Sequence Identifier (SEQ ID NO:) and Sequence or comments
CDRH1 SEQ ID NO:426
CDRH2 458
IIDPDDSYTRYSPSFQG
CDRH3 SEQ ID NO:428
CDRL1 SEQ ID NO:429
CDRL2 SEQ ID NO:430
CDRL3 459
ATWGSEDQV
VH 460
EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQV TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS
VL 461
SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCATWGSEDQVFGGGTKLTVL
Heavy chain 462
EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQV
TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTS
GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNH KPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Light chain 463
SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCATWGSEDQVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCL ISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTV EKTVAPTECS
PN encoding 464
SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:460 GCAAAGGTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGT
CTCGAGTGGATGGGCATTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTTTCAGGG
ACAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAG
CGAGCGATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGG I I I I GATATTTGGGGCCAA
GGCACCCTGGTGACGGTTAGCTCA
PN encoding 465
SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:461 GTAGCGGCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCA
GTTCTTGTGATTTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC
AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT
GCGCTACTTGGGGTTCTGAGGATCAGGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTA
PN encoding 466
SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:462 GCAAAGGTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGT
CTCGAGTGGATGGGCATTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTTTCAGGG
ACAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAG
CGAGCGATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGG I I I I GATATTTGGGGCCAA
GGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCT
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCG
AACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTG
TCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGG
CACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT
GAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGG
GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAG
GTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGG
ACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC
GGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA
GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC
CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC
TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA
GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTAC
AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC
ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
PN encoding 467
SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NQ:463 GTAGCGGCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCA
GTTCTTGTGATTTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC
AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT
GCGCTACTTGGGGTTCTGAGGATCAGGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCA GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG
GCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG
ATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTA
CGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC
CAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
Optimized PN 468
encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGC ID NO:462 TGCAAGGGCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAG
GGCCTGGAGTGGATGGGCATCATCGACCCCGACGACAGCTACACCAGGTACAGCCCCAGCTTCC
AGGGCCAGGTGACCATCAGCGCCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCC
TGAAGGCCAGCGACACCGCCATGTACTACTGCGCCAGATACGAGTACGGCGGCTTCGACATCTG
GGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCCCCT
GGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGACTA
CTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTT
CCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAG
CAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGAC
AAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAA
GCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCA
GGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCA
ACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTACA
ACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGA
ATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGCC
AAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG
AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGG
AGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCA
GCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAG
CTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCC
GGCAAG
Optimized PN 469
encoding SEQ AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGC ID NO:463 TGCAGCGGCGACAATATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCCC
CCGTGCTGGTGATCTACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCA
GCAACTCCGGCAACACCGCCACCCTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACT
ACTACTGCGCCACCTGGGGCTCAGAGGACCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTGC
TGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCTGCAGG
CCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTGGCCTG
GAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAGAGCAA
CAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTC
CTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTG
CAGC
Antibody 8114 Sequence Identifier (SEQ ID NO:) and Sequence or comments
CDRH1 470
SYYIG
CDRH2 471
11 D PTDS QTAYS PS FQG
CDRH3 472
YMMRGFDH
CDRL1 473
SGDSLGDYYAY CDRL2 474
KDNNRPS
CDRL3 475
QTWDTGESGV
VH 476
EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQ VTISADKSISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS
VL 477
SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCQTWDTGESGVFGGGTKLTVL
Heavy chain 478
EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQ
VTISADKSISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKS
TSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNV
NHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHE
DPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTI
SKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGS
FF LYS KLTVD KS RWQQG N VFSCSVM H EAL H N HYTQ KSLSLSPGK
Light chain 479
SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCQTWDTGESGVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLV CLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGS TVEKTVAPTECS
PN encoding 480
SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:476 GCAAAGGTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAG
GGTCTCGAGTGGATGGGCATTATTGATCCTACTGATTCTCAGACTGCTTATTCTCCTTC 1 1 1 1 CAG GGTCAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAA AGCGAGCGATACGGCCATGTATTATTGCGCGCGTTATATGATGCGTGG 1 1 1 1 GATCATTGGGGCC AAGGCACCCTGGTGACGGTTAGCTCA
PN encoding 481
SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:478 GTAGCGGCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCA
GTTCTTGTGATTTATAAGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC
AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT
GCCAGACTTGGGATACTGGTGAGTCTGGTGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTA
PN encoding 482
SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NQ:479 GCAAAGGTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAG
GGTCTCGAGTGGATGGGCATTATTGATCCTACTGATTCTCAGACTGCTTATTCTCCTTC I I I I CAG
GGTCAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAA
AGCGAGCGATACGGCCATGTATTATTGCGCGCGTTATATGATGCGTGG I I I I GATCATTGGGGCC
AAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACC
CTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC
GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCT
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGG
GCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGT
TGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGG
GGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGA
GGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTG GACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTAC
CGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCA
AGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCC
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGC
CTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGC
AGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTA
CAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATG
CATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA
PN encoding 483
SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:480 GTAGCGGCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCA
GTTCTTGTGATTTATAAGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC
AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT
GCCAGACTTGGGATACTGGTGAGTCTGGTGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGG
TCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAAC
AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGG
CAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAA
GTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGC
TGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA
Optimized PN 484
encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGC ID NO:479 TGCAAGGGCAGCGGCTACAGCTTCACCAGCTACTACATCGGCTGGGTGCGGCAGATGCCCGGC
AAGGGCCTGGAGTGGATGGGCATCATCGACCCCACCGACAGCCAGACCGCCTACAGCCCCAGC
TTCCAGGGCCAGGTGACCATCAGCGCCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCA
GCCTGAAGGCCAGCGACACCGCCATGTACTACTGCGCCCGGTACATGATGAGGGGCTTCGACCA
CTGGGGTCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCC
CCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGA
CTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACAC
CTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAG
CAGCAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTG
GACAAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCC
GAAGCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCA
GCAGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGT
TCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGT
ACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAA
AGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAG
GCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACC
AAGAACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGT
GGGAGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACG
GCAGCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTT
CAGCTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCA
CCCGGCAAG
Optimized PN 485
encoding SEQ AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGC ID NO:480 TGCAGCGGCGACAGCCTGGGCGACTACTACGCCTACTGGTATCAGCAGAAGCCCGGCCAGGCC
CCCGTGCTGGTGATCTACAAGGACAACAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCA
GCAACAGCGGCAACACCGCCACCCTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACT
ACTACTGCCAGACCTGGGACACCGGCGAGTCAGGCGTGTTCGGCGGAGGGACCAAGCTGACCG
TGCTGGGTCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCTGC
AGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTGGC CTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAGAG CAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAG GTCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGA GTGCAGC
Other antibodies of the invention include those where the amino acids or nucleic acids encoding the amino acids have been mutated, yet have at least 60, 65, 70, 75, 80, 85, 90, or 95 percent identity to the sequences described in Table 1. Some
embodiments include mutant amino acid sequences wherein no more than 1 , 2, 3, 4 or 5 amino acids have been mutated in the variable regions when compared with the variable regions depicted in the sequence described in Table 1 , while retaining substantially the same antigen binding activity.
Since each of these antibodies can bind to Factor P, the VH, VL, full length light chain, and full length heavy chain sequences (amino acid sequences and the nucleotide sequences encoding the amino acid sequences) can be "mixed and matched" to create other Factor P-binding antibodies of the invention. Such "mixed and matched" Factor P- binding antibodies can be tested using the binding assays known in the art (e.g., ELISAs, and other assays described in the Example section). When these chains are mixed and matched, a VH sequence from a particular VH/VL pairing should be replaced with a structurally similar VH sequence. Likewise a full length heavy chain sequence from a particular full length heavy chain / full length light chain pairing should be replaced with a structurally similar full length heavy chain sequence. Likewise, a VL sequence from a particular VH/VL pairing should be replaced with a structurally similar VL sequence. Likewise a full length light chain sequence from a particular full length heavy chain / full length light chain pairing should be replaced with a structurally similar full length light chain sequence. Accordingly, in one aspect, the invention provides an isolated antibody or antigen binding region thereof having: a heavy chain variable domain comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273, and a light chain variable domain comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274 wherein the antibody specifically binds to Factor P (e.g., human and/or cynomolgus Factor P).
In another aspect, the invention provides (i) an isolated antibody having: a full length heavy chain comprising an amino acid sequence that has been optimized for expression in a mammalian cell selected from the group consisting of SEQ ID NOs: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 and 275, and a full length light chain comprising an amino acid sequence that has been optimized for expression in a mammalian cell selected from the group consisting of SEQ ID NOs: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 and 276; or (ii) a functional protein comprising an antigen binding portion thereof.
The terms "complementarity determining region," and "CDR," as used herein refer to the sequences of amino acids within antibody variable regions which confer antigen specificity and binding affinity. In general, there are three CDRs in each heavy chain variable region (HCDR1 , HCDR2, HCDR3) and three CDRs in each light chain variable region (LCDR1 , LCDR2, LCDR3).
The precise amino acid sequence boundaries of a given CDR can be readily determined using any of a number of well-known schemes, including those described by Kabat et al. (1991 ), "Sequences of Proteins of Immunological Interest," 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD ("Kabat" numbering scheme), Al-Lazikani et al., (1997) JMB 273,927-948 ("Chothia" numbering scheme).
For example, under Kabat, the CDR amino acid residues in the heavy chain variable domain (VH) are numbered 31-35 (HCDR1 ), 50-65 (HCDR2), and 95-102 (HCDR3); and the CDR amino acid residues in the light chain variable domain (VL) are numbered 24-34 (LCDR1 ), 50-56 (LCDR2), and 89-97 (LCDR3). Under Chothia the CDR amino acids in the VH are numbered 26-32 (HCDR1 ), 52-56 (HCDR2), and 95-102 (HCDR3); and the amino acid residues in VL are numbered 26-32 (LCDR1 ), 50-52 (LCDR2), and 91 -96 (LCDR3). By combining the CDR definitions of both Kabat and Chothia, the CDRs consist of amino acid residues 26-35 (HCDR1 ), 50-65 (HCDR2), and 95-102 (HCDR3) in human VH and amino acid residues 24-34 (LCDR1 ), 50-56 (LCDR2), and 89-97 (LCDR3) in human VL.
In another aspect, the present invention provides Factor P binding antibodies that comprise the heavy chain and light chain CDR1 s, CDR2s, and CDR3s as described in Table 1 , or combinations thereof. The amino acid sequences of the VH CDR1 s of the antibodies are shown in SEQ ID NOs: 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, or 267. The amino acid sequences of the VH CDR2s of the antibodies and are shown in SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, or 268. The amino acid sequences of the VH CDR3s of the antibodies are shown in SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, or 269. The amino acid sequences of the VL CDR1 s of the antibodies are shown in SEQ I D NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, or 270. The amino acid sequences of the VL CDR2s of the antibodies are shown in SEQ I D NOs: 5, 19, 33, 47, 61 , 75, 89, 103, 1 17, 131 , 145, 159, 173, 187, 201 , 215, 229, 243, 257, or 271 . The amino acid sequences of the VL CDR3s of the antibodies are shown in SEQ I D NOs: 6, 20, 34, 48, 62, 76, 90, 104, 1 18, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, or 272. These CDR regions are delineated using the Kabat system.
Alternatively, as defined using the Chothia system (Al-Lazikani et al., (1997) JMB 273,927-948) the amino acid sequences of the VH CDR1 s of the antibodies are shown in SEQ I D NOs: 281 , 287, 293, 299, 305, 31 1 , 317, 323, 329, 335, 341 , 347, 353, 359, 365, 371 , 377, 383, 389, or 395. The amino acid sequences of the VH CDR2s of the antibodies and are shown in SEQ I D NOs: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, or 396. The amino acid sequences of the VH CDR3s of the antibodies are shown in SEQ I D NOs: 283, 289, 295, 301 , 307, 313, 319, 325, 331 , 337, 343, 349, 355, 361 , 367, 373, 379, 385, 391 , or 397. The amino acid sequences of the VL CDR1 s of the antibodies are shown in SEQ I D NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, or 398. The amino acid sequences of the VL CDR2s of the antibodies are shown in SEQ I D NOs: 285, 291 , 297, 303, 309, 315, 321 , 327, 333, 339, 345, 351 , 357, 363, 369, 375, 381 , 387, 393, or 399. The amino acid sequences of the VL CDR3s of the antibodies are shown in SEQ I D NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, or 400.
Given that each of these antibodies can bind to Factor P and that antigen-binding specificity is provided primarily by the CDR1 , 2 and 3 regions, the VH CDR1 , 2 and 3 sequences and VL CDR1 , 2 and 3 sequences can be "mixed and matched" (i.e., CDRs from different antibodies can be mixed and matched, although each antibody preferably contains a VH CDR1 , 2 and 3 and a VL CDR1 , 2 and 3 to create other Factor P binding binding molecules of the invention. Such "mixed and matched" Factor P binding antibodies can be tested using the binding assays known in the art and those described in the Examples (e.g., ELISAs, SET, Biacore). When VH CDR sequences are mixed and matched, the CDR1 , CDR2 and/or CDR3 sequence from a particular VH sequence should be replaced with a structurally similar CDR sequence(s). Likewise, when VL CDR sequences are mixed and matched, the CDR1 , CDR2 and/or CDR3 sequence from a particular VL sequence should be replaced with a structurally similar CDR sequence(s). It will be readily apparent to the ordinarily skilled artisan that novel VH and VL sequences can be created by substituting one or more VH and/or VL CDR region sequences with structurally similar sequences from the CDR sequences shown herein for monoclonal antibodies of the present invention. In addition to the foregoing, in one embodiment, the antigen binding fragments of the antibodies described herein can comprise a VH CDR1 , 2, and 3, or a VL CDR 1 , 2, and 3, wherein the fragment binds to Factor P as a single variable domain.
In certain embodiments of the invention, the antibodies or antigen binding fragments thereof may have the heavy and light chain sequences of the Fabs described in Table 1 . More specifically, the antibody or antigen binding fragment thereof may have the heavy and light sequence of Fab NVS962, NVS963, NVS964, NVS965, NVS966, NVS967, NVS805, NVS806, NVS807, NVS808, NVS809, NVS962-S, NVS962-Q, NVS962-S31A, NVS962-G, NVS962-T, NVS965-S, NVS965-T, or NVS965-Q.
In other enbodiments of the invention the antibody or antigen binding fragment in that specifically binds Factor P comprises a heavy chain variable region CDR1 , a heavy chain variable region CDR2, a heavy chain variable region CDR3, a light chain variable region CDR1 , a light chain variable region CDR2, and a light chain variable region CDR3 as defined by Kabat and described in Table 1. In still other embodiments of the invention the antibody or antigen binding fragment in that specifically binds Factor P comprises a heavy chain variable region CDR1 , a heavy chain variable region CDR2, a heavy chain variable region CDR3, a light chain variable region CDR1 , a light chain variable region CDR2, and a light chain variable region CDR3 as defined by Chothia and described in Table 1.
In a specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO:1 ; a heavy chain variable region CDR2 of SEQ ID NO: 2; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 6. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 15; a heavy chain variable region CDR2 of SEQ ID NO: 16; a heavy chain variable region CDR3 of SEQ ID NO: 17; a light chain variable region CDR1 of SEQ ID NO: 18; a light chain variable region CDR2 of SEQ ID NO: 19; and a light chain variable region CDR3 of SEQ ID NO: 20. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 29; a heavy chain variable region CDR2 of SEQ ID NO: 30; a heavy chain variable region CDR3 of SEQ ID NO: 31 ; a light chain variable region CDR1 of SEQ ID NO: 32; a light chain variable region CDR2 of SEQ ID NO: 33; and a light chain variable region CDR3 of SEQ ID NO: 34. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 43; a heavy chain variable region CDR2 of SEQ ID NO: 44; a heavy chain variable region CDR3 of SEQ ID NO: 45; a light chain variable region CDR1 of SEQ ID NO: 46; a light chain variable region CDR2 of SEQ ID NO: 47; and a light chain variable region CDR3 of SEQ ID NO: 48. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 57; a heavy chain variable region CDR2 of SEQ ID NO: 58; a heavy chain variable region CDR3 of SEQ ID NO: 59; a light chain variable region CDR1 of SEQ ID NO: 60; a light chain variable region CDR2 of SEQ ID NO: 61 ; and a light chain variable region CDR3 of SEQ ID NO: 62. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 71 ; a heavy chain variable region CDR2 of SEQ ID NO: 72; a heavy chain variable region CDR3 of SEQ ID NO: 73; a light chain variable region CDR1 of SEQ ID NO: 74; a light chain variable region CDR2 of SEQ ID NO: 75; and a light chain variable region CDR3 of SEQ ID NO: 76. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 85; a heavy chain variable region CDR2 of SEQ ID NO: 86; a heavy chain variable region CDR3 of SEQ ID NO: 87; a light chain variable region CDR1 of SEQ ID NO: 88; a light chain variable region CDR2 of SEQ ID NO: 89; and a light chain variable region CDR3 of SEQ ID NO: 90. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 99; a heavy chain variable region CDR2 of SEQ ID NO: 100; a heavy chain variable region CDR3 of SEQ ID NO: 101 ; a light chain variable region CDR1 of SEQ ID NO: 102; a light chain variable region CDR2 of SEQ ID NO: 103; and a light chain variable region CDR3 of SEQ ID NO: 104. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 1 13; a heavy chain variable region CDR2 of SEQ ID NO: 1 14; a heavy chain variable region CDR3 of SEQ ID NO: 1 15; a light chain variable region CDR1 of SEQ ID NO: 1 16; a light chain variable region CDR2 of SEQ ID NO: 1 17; and a light chain variable region CDR3 of SEQ ID NO: 1 18. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 127; a heavy chain variable region CDR2 of SEQ ID NO: 128; a heavy chain variable region CDR3 of SEQ ID NO: 129; a light chain variable region CDR1 of SEQ ID NO: 130; a light chain variable region CDR2 of SEQ ID NO: 131 ; and a light chain variable region CDR3 of SEQ ID NO: 132. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 141 ; a heavy chain variable region CDR2 of SEQ ID NO: 142; a heavy chain variable region CDR3 of SEQ ID NO: 143; a light chain variable region CDR1 of SEQ ID NO: 144; a light chain variable region CDR2 of SEQ ID NO: 145; and a light chain variable region CDR3 of SEQ ID NO: 146. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 155; a heavy chain variable region CDR2 of SEQ ID NO: 156; a heavy chain variable region CDR3 of SEQ ID NO: 157; a light chain variable region CDR1 of SEQ ID NO: 158; a light chain variable region CDR2 of SEQ ID NO: 159; and a light chain variable region CDR3 of SEQ ID NO: 160. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 169; a heavy chain variable region CDR2 of SEQ ID NO: 170; a heavy chain variable region CDR3 of SEQ ID NO: 171 ; a light chain variable region CDR1 of SEQ ID NO: 172; a light chain variable region CDR2 of SEQ ID NO: 173; and a light chain variable region CDR3 of SEQ ID NO: 174. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 183; a heavy chain variable region CDR2 of SEQ ID NO: 184; a heavy chain variable region CDR3 of SEQ ID NO: 185; a light chain variable region CDR1 of SEQ ID NO: 186; a light chain variable region CDR2 of SEQ ID NO: 187; and a light chain variable region CDR3 of SEQ ID NO: 188. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 197; a heavy chain variable region CDR2 of SEQ ID NO: 198; a heavy chain variable region CDR3 of SEQ ID NO: 199; a light chain variable region CDR1 of SEQ ID NO: 200; a light chain variable region CDR2 of SEQ ID NO: 201 ; and a light chain variable region CDR3 of SEQ ID NO: 202. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 21 1 ; a heavy chain variable region CDR2 of SEQ ID NO: 212; a heavy chain variable region CDR3 of SEQ ID NO: 213; a light chain variable region CDR1 of SEQ ID NO: 214; a light chain variable region CDR2 of SEQ ID NO: 215; and a light chain variable region CDR3 of SEQ ID NO: 216. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 225; a heavy chain variable region CDR2 of SEQ ID NO: 226; a heavy chain variable region CDR3 of SEQ ID NO: 227; a light chain variable region CDR1 of SEQ ID NO: 228; a light chain variable region CDR2 of SEQ ID NO: 229; and a light chain variable region CDR3 of SEQ ID NO: 230. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 239; a heavy chain variable region CDR2 of SEQ ID NO: 240; a heavy chain variable region CDR3 of SEQ ID NO: 241 ; a light chain variable region CDR1 of SEQ ID NO: 242; a light chain variable region CDR2 of SEQ ID NO: 243; and a light chain variable region CDR3 of SEQ ID NO: 244. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 253; a heavy chain variable region CDR2 of SEQ ID NO: 254; a heavy chain variable region CDR3 of SEQ ID NO: 255; a light chain variable region CDR1 of SEQ ID NO: 256; a light chain variable region CDR2 of SEQ ID NO: 257; and a light chain variable region CDR3 of SEQ ID NO: 258. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 267; a heavy chain variable region CDR2 of SEQ ID NO: 268; a heavy chain variable region CDR3 of SEQ ID NO: 269; a light chain variable region CDR1 of SEQ ID NO: 270; a light chain variable region CDR2 of SEQ ID NO: 271 ; and a light chain variable region CDR3 of SEQ ID NO: 271.
In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 281 ; a heavy chain variable region CDR2 of SEQ ID NO: 282; a heavy chain variable region CDR3 of SEQ ID NO: 283; a light chain variable region CDR1 of SEQ ID NO: 284; a light chain variable region CDR2 of SEQ ID NO: 285; and a light chain variable region CDR3 of SEQ ID NO: 286. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 287; a heavy chain variable region CDR2 of SEQ ID NO: 288; a heavy chain variable region CDR3 of SEQ ID NO: 289; a light chain variable region CDR1 of SEQ ID NO: 290; a light chain variable region CDR2 of SEQ ID NO: 291 ; and a light chain variable region CDR3 of SEQ ID NO: 292. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 293; a heavy chain variable region CDR2 of SEQ ID NO: 294; a heavy chain variable region CDR3 of SEQ ID NO: 295; a light chain variable region CDR1 of SEQ ID NO: 296; a light chain variable region CDR2 of SEQ ID NO: 297; and a light chain variable region CDR3 of SEQ ID NO: 298. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 299; a heavy chain variable region CDR2 of SEQ ID NO: 300; a heavy chain variable region CDR3 of SEQ ID NO: 301 ; a light chain variable region CDR1 of SEQ ID NO: 302; a light chain variable region CDR2 of SEQ ID NO: 303; and a light chain variable region CDR3 of SEQ ID NO: 304. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 305; a heavy chain variable region CDR2 of SEQ ID NO: 306; a heavy chain variable region CDR3 of SEQ ID NO: 307; a light chain variable region CDR1 of SEQ ID NO: 308; a light chain variable region CDR2 of SEQ ID NO: 309; and a light chain variable region CDR3 of SEQ ID NO: 310. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 31 1 ; a heavy chain variable region CDR2 of SEQ ID NO: 312; a heavy chain variable region CDR3 of SEQ ID NO: 313; a light chain variable region CDR1 of SEQ ID NO: 314; a light chain variable region CDR2 of SEQ ID NO: 315; and a light chain variable region CDR3 of SEQ ID NO: 316. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 317; a heavy chain variable region CDR2 of SEQ ID NO: 318; a heavy chain variable region CDR3 of SEQ ID NO: 319; a light chain variable region CDR1 of SEQ ID NO: 320; a light chain variable region CDR2 of SEQ ID NO: 321 ; and a light chain variable region CDR3 of SEQ ID NO: 322. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 323; a heavy chain variable region CDR2 of SEQ ID NO: 324; a heavy chain variable region CDR3 of SEQ ID NO: 325; a light chain variable region CDR1 of SEQ ID NO: 326; a light chain variable region CDR2 of SEQ ID NO: 327; and a light chain variable region CDR3 of SEQ ID NO: 328. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 329; a heavy chain variable region CDR2 of SEQ ID NO: 330; a heavy chain variable region CDR3 of SEQ ID NO: 331 ; a light chain variable region CDR1 of SEQ ID NO: 332; a light chain variable region CDR2 of SEQ ID NO: 333; and a light chain variable region CDR3 of SEQ ID NO: 334. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 335; a heavy chain variable region CDR2 of SEQ ID NO: 336; a heavy chain variable region CDR3 of SEQ ID NO: 337; a light chain variable region CDR1 of SEQ ID NO: 338; a light chain variable region CDR2 of SEQ ID NO: 339; and a light chain variable region CDR3 of SEQ ID NO: 340. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 341 ; a heavy chain variable region CDR2 of SEQ ID NO: 342; a heavy chain variable region CDR3 of SEQ ID NO: 343; a light chain variable region CDR1 of SEQ ID NO: 344; a light chain variable region CDR2 of SEQ ID NO: 345; and a light chain variable region CDR3 of SEQ ID NO: 346. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 347; a heavy chain variable region CDR2 of SEQ ID NO: 348; a heavy chain variable region CDR3 of SEQ ID NO: 349; a light chain variable region CDR1 of SEQ ID NO: 350; a light chain variable region CDR2 of SEQ ID NO: 351 ; and a light chain variable region CDR3 of SEQ ID NO: 352. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 353; a heavy chain variable region CDR2 of SEQ ID NO: 354; a heavy chain variable region CDR3 of SEQ ID NO: 355; a light chain variable region CDR1 of SEQ ID NO: 356; a light chain variable region CDR2 of SEQ ID NO: 357; and a light chain variable region CDR3 of SEQ ID NO: 358. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 359; a heavy chain variable region CDR2 of SEQ ID NO: 360; a heavy chain variable region CDR3 of SEQ ID NO: 361 ; a light chain variable region CDR1 of SEQ ID NO: 362; a light chain variable region CDR2 of SEQ ID NO: 363; and a light chain variable region CDR3 of SEQ ID NO: 364. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 365; a heavy chain variable region CDR2 of SEQ ID NO: 366; a heavy chain variable region CDR3 of SEQ ID NO: 367; a light chain variable region CDR1 of SEQ ID NO: 368; a light chain variable region CDR2 of SEQ ID NO: 369; and a light chain variable region CDR3 of SEQ ID NO: 370. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 371 ; a heavy chain variable region CDR2 of SEQ ID NO: 372; a heavy chain variable region CDR3 of SEQ ID NO: 373; a light chain variable region CDR1 of SEQ ID NO: 374; a light chain variable region CDR2 of SEQ ID NO: 375; and a light chain variable region CDR3 of SEQ ID NO: 376. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 377; a heavy chain variable region CDR2 of SEQ ID NO: 378; a heavy chain variable region CDR3 of SEQ ID NO: 379; a light chain variable region CDR1 of SEQ ID NO: 380; a light chain variable region CDR2 of SEQ ID NO: 381 ; and a light chain variable region CDR3 of SEQ ID NO: 382. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 383; a heavy chain variable region CDR2 of SEQ ID NO: 384; a heavy chain variable region CDR3 of SEQ ID NO: 385; a light chain variable region CDR1 of SEQ ID NO: 386; a light chain variable region CDR2 of SEQ ID NO: 387; and a light chain variable region CDR3 of SEQ ID NO: 388. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 389; a heavy chain variable region CDR2 of SEQ ID NO: 390; a heavy chain variable region CDR3 of SEQ ID NO: 391 ; a light chain variable region CDR1 of SEQ ID NO: 392; a light chain variable region CDR2 of SEQ ID NO: 393; and a light chain variable region CDR3 of SEQ ID NO: 394. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 395; a heavy chain variable region CDR2 of SEQ ID NO: 396; a heavy chain variable region CDR3 of SEQ ID NO: 397; a light chain variable region CDR1 of SEQ ID NO: 398; a light chain variable region CDR2 of SEQ ID NO: 399; and a light chain variable region CDR3 of SEQ ID NO: 400.
In certain embodiments, the invention includes antibodies or antigen binding fragments that specifically binds to Factor P as described in Table 1. In a preferred embodiment, the antibody, or antigen binding fragment, that binds Factor P is Fab NVS962, NVS963, NVS964, NVS965, NVS966, NVS967, NVS804, NVS805, NVS806, NVS807, NVS808, NVS809, NVS962-S, NVS962-Q, NVS962-G, NVS962-T, NVS962- S31A, NVS965-T, NVS965-Q, or NVS965-S.
As used herein, a human antibody comprises heavy or light chain variable regions or full length heavy or light chains that are "the product of" or "derived from" a particular germline sequence if the variable regions or full length chains of the antibody are obtained from a system that uses human germline immunoglobulin genes. Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of interest or screening a human immunoglobulin gene library displayed on phage with the antigen of interest. A human antibody that is "the product of" or "derived from" a human germline immunoglobulin sequence can be identified as such by comparing the amino acid sequence of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence {i.e., greatest % identity) to the sequence of the human antibody. A human antibody that is "the product of" or "derived from" a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally occurring somatic mutations or intentional introduction of site-directed mutations. However, in the VH or VL framework regions, a selected human antibody typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline
immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences). In certain cases, a human antibody may be at least 60%, 70%, 80%, 90%, or at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene. Typically, a recombinant human antibody will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene in the VH or VL framework regions. In certain cases, the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid sequence encoded by the germline immunoglobulin gene. Examples of human germline immunoglobulin genes include, but are not limited to the variable domain germline fragments described below, as well as DP47 and DPK9.
Homologous antibodies
In yet another embodiment, the present invention provides an antibody, or an antigen binding fragment thereof, comprising amino acid sequences that are homologous to the sequences described in Table 1 , and the antibody binds to a Factor P protein (e.g., human and/or cynomolgus Factor P), and retains the desired functional properties of those antibodies described in Table 1.
For example, the invention provides an isolated antibody, or a functional antigen binding fragment thereof, comprising a heavy chain variable domain and a light chain variable domain, wherein the heavy chain variable domain comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273; the light chain variable domain comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274; and the antibody specifically binds to Factor P (e.g., human and/or cynomolgus Factor P).
In other embodiments, the VH and/or VL amino acid sequences may be 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1 . In other embodiments, the VH and/or VL amino acid sequences may be identical except for an amino acid substitution in no more than 1 ,2,3,4 or 5 amino acid positions. An antibody having VH and VL regions having high (/'. e., 80% or greater) identity to the VH and VL regions of those described in Table 1 can be obtained by mutagenesis (e.g., site-directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 or 273 and SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274, respectively, followed by testing of the encoded altered antibody for retained function using the functional assays described herein.
In other embodiments, the full length heavy chain and/or full length light chain amino acid sequences may be 50% 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1 . An antibody having a full length heavy chain and full length light chain having high (i.e., 80% or greater) identity to the full length heavy chains of any of SEQ ID NOs : 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275, and full length light chains of any of SEQ ID NOs 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276, can be obtained by mutagenesis (e.g., site-directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding such polypeptides, followed by testing of the encoded altered antibody for retained function using the functional assays described herein.
In other embodiments, the full length heavy chain and/or full length light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1 .
In other embodiments, the variable regions of heavy chain and/or the variable regions of light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1 .
As used herein, the percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity equals number of identical positions/total number of positions x 100), taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences. The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm, as described in the non-limiting examples below. Additionally or alternatively, the protein sequences of the present invention can further be used as a "query sequence" to perform a search against public databases to, for example, identify related sequences. For example, such searches can be performed using the BLAST program (version 2.0) of Altschul, et al., 1990 J.Mol. Biol. 215:403-10.
Antibodies with Conservative Modifications
In certain embodiments, an antibody of the invention has a heavy chain variable region comprising CDR1 , CDR2, and CDR3 sequences and a light chain variable region comprising CDR1 , CDR2, and CDR3 sequences, wherein one or more of these CDR sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the Factor P-binding antibodies of the invention.
Accordingly, the invention provides an isolated antibody, or a antigen binding fragment thereof, consisting of a heavy chain variable region comprising CDR1 , CDR2, and CDR3 sequences and a light chain variable region comprising CDR1 , CDR2, and CDR3 sequences, wherein: the heavy chain variable region CDR1 amino acid sequences are selected from the group consisting of SEQ ID NOs: 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13,
127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, and 267, and conservative
modifications thereof; the heavy chain variable region CDR2 amino acid sequences are selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14,
128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268, and conservative
modifications thereof; the heavy chain variable region CDR3 amino acid sequences are selected from the group consisting of SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15,
129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, and 269, and conservative
modifications thereof; the light chain variable regions CDR1 amino acid sequences are selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16,
130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270, and conservative
modifications thereof; the light chain variable regions CDR2 amino acid sequences are selected from the group consisting of SEQ ID NOs: 5, 19, 33, 47, 61 , 75, 89, 103, 1 17,
131 , 145, 159, 173, 187, 201 , 215, 229, 243, 257, and 271 , and conservative
modifications thereof; the light chain variable regions of CDR3 amino acid sequences are selected from the group consisting of SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 1 18,
132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272, and conservative
modifications thereof; and the antibody or antigen binding fragment thereof specifically binds to Factor P. In other embodiments, the antibody of the invention is optimized for expression in a mammalian cell has a full length heavy chain sequence and a full length light chain sequence, wherein one or more of these sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the Factor P binding antibodies of the invention. Accordingly, the invention provides an isolated antibody optimized for expression in a mammalian cell consisting of a full length heavy chain and a full length light chain wherein the full length heavy chain has amino acid sequences selected from the group of SEQ ID NOs: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 and 275, and conservative
modifications thereof; and the full length light chain has amino acid sequences selected from the group of SEQ ID NOs: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, and 276, and conservative modifications thereof; and the antibody specifically binds to Factor P (e.g., human and/or cynomolgus Factor P).
Antibodies That Bind to the Same Epitope
The present invention provides antibodies that bind to the same epitope as the Factor P binding antibodies described in Table 1. Additional antibodies can therefore be identified based on their ability to compete (e.g., to competitively inhibit the binding of, in a statistically significant manner) with other antibodies of the invention in Factor P binding assays (such as those described in the Examples). The ability of a test antibody to inhibit the binding of antibodies of the present invention to a Factor P protein demonstrates that the test antibody can compete with that antibody for binding to Factor P; such an antibody may, according to non-limiting theory, bind to the same or a related (e.g., a structurally similar or spatially proximal) epitope on the Factor P protein as the antibody with which it competes. In a certain embodiment, the antibody that binds to the same epitope on Factor P as the antibodies of the present invention is a human monoclonal antibody. Such human monoclonal antibodies can be prepared and isolated as described herein. As used herein, an antibody "competes" for binding when the competing antibody inhibits Factor P binding of an antibody or antigen binding fragment of the invention by more than 50%, in the presence of an equimolar concentration of competing antibody.
In other embodiments the antibodies or antigen binding fragments of the invention bind the Thrombospondin type 5 repeat (TSR 5) domain of Factor P (SEQ ID NO: 406). In other embodiments the antibodies or antigen binding fragments of the invention bind a region of the Factor P TSR5 domain comprising SEQ ID NO: 407. Still in other embodiments the region comprises SEQ ID NO: 408.
In other embodiments of the invention the isolated antibodies or antigen binding fragments bind an epitope comprising SEQ ID NO: 407, and in other embodiments the epitope comprises SEQ ID NO: 408. In other embodiments of the invention, the antibodies or antigen binding fragments bind a peptide according to SEQ ID NO: 407 and in still other embodiments the Factor P epitope includes SEQ ID NO: 408.
Engineered and Modified Antibodies
An antibody of the invention further can be prepared using an antibody having one or more of the VH and/or VL sequences shown herein as starting material to engineer a modified antibody, which modified antibody may have altered properties from the starting antibody. An antibody can be engineered by modifying one or more residues within one or both variable regions (/'. e., VH and/or VL), for example within one or more CDR regions and/or within one or more framework regions. Additionally or alternatively, an antibody can be engineered by modifying residues within the constant region(s), for example to alter the effector function(s) of the antibody.
One type of variable region engineering that can be performed is CDR grafting. Antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions (CDRs). For this reason, the amino acid sequences within CDRs are more diverse between individual antibodies than sequences outside of CDRs. Because CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally occurring antibody grafted onto framework sequences from a different antibody with different properties (see, e.g., Riechmann, L. et al., 1998 Nature 332:323-327; Jones, P. et al., 1986 Nature 321 :522-525; Queen, C. et al., 1989 Proc. Natl. Acad., U.S.A.
86:10029-10033; U.S. Patent No. 5,225,539 to Winter, and U.S. Patent Nos. 5,530, 101 ; 5,585,089; 5,693,762 and 6, 180,370 to Queen et al.)
Accordingly, another embodiment of the invention pertains to an isolated antibody, or an antigen binding fragment thereof, comprising a heavy chain variable region comprising CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, and 267; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268; CDR3 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, and 269, respectively; and a light chain variable region having CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 5, 19, 33, 47, 61 , 75, 89, 103, 1 17, 131 , 145, 159, 173, 187, 201 , 215, 229, 243, 257, and 271 ; and CDR3 sequences consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 1 18, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272, respectively. Thus, such antibodies contain the VH and VL CDR sequences of monoclonal antibodies, yet may contain different framework sequences from these antibodies.
Alternatively, another embodiment of the invention pertains to an isolated antibody, or an antigen binding fragment thereof, comprising a heavy chain variable region comprising CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 281 , 287, 293, 299, 305, 31 1 , 317, 323, 329, 335, 341 , 347, 353, 359, 365, 371 , 377, 383, 389, and 395; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, and 396; CDR3 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 283, 289, 295, 301 , 307, 313, 319, 325, 331 , 337, 343, 349, 355, 361 , 367, 373, 379, 385, 391 , and 397, respectively; and a light chain variable region having CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, and 398; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 285, 291 , 297, 303, 309, 315, 321 , 327, 333, 339, 345, 351 , 357, 363, 369, 375, 381 , 387, 393, and 399; and CDR3 sequences consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, and 400, respectively. Thus, such antibodies contain the VH and VL CDR sequences of monoclonal antibodies, yet may contain different framework sequences from these antibodies.
Such framework sequences can be obtained from public DNA databases or published references that include germline antibody gene sequences. For example, germline DNA sequences for human heavy and light chain variable region genes can be found in the "VBase" human germline sequence database (available on the world wide web at mrc- cpe.cam.ac.uk/vbase), as well as in Kabat, E. A., et al., 1991 Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91 -3242; Tomlinson, I. M., et al., 1992 J. Mol. Biol.
227:776-798; and Cox, J. P. L. et al., 1994 Eur. J Immunol. 24:827-836; the contents of each of which are expressly incorporated herein by reference.
An example of framework sequences for use in the antibodies of the invention are those that are structurally similar to the framework sequences used by selected antibodies of the invention, e.g., consensus sequences and/or framework sequences used by monoclonal antibodies of the invention. The VH CDR1 , 2 and 3 sequences, and the VL CDR1 , 2 and 3 sequences, can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations as compared to the germline sequences. For example, it has been found that in certain instances it is beneficial to mutate residues within the framework regions to maintain or enhance the antigen binding ability of the antibody (see e.g., U.S. Patent Nos. 5,530, 101 ; 5,585,089; 5,693,762 and 6, 180,370 to Queen et al). Frameworks that can be utilized as scaffolds on which to build the antibodies and antigen binding fragments described herein include, but are not limited to VH1 A, VH1 B, VH3, Vk1 , VI2, and Vk2. Additional frameworks are known in the art and may be found, for example, in the vBase data base on the world wide web at vbase.mrc- cpe.cam.ac.uk/index.php?&MMN_position=1 :1.
Accordingly, an embodiment of the invention relates to isolated Factor P binding antibodies, or antigen binding fragments thereof, comprising a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 and 273, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions in the framework region of such sequences, and further comprising a light chain variable region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions in the framework region of such sequences. Another type of variable region modification is to mutate amino acid residues within the VH and/or VL CDR1 , CDR2 and/or CDR3 regions to thereby improve one or more binding properties (e.g., affinity) of the antibody of interest, known as "affinity maturation." Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of interest, can be evaluated in in vitro or in vivo assays as described herein and provided in the Examples. Conservative modifications (as discussed above) can be introduced. The mutations may be amino acid substitutions, additions or deletions.
Moreover, typically no more than one, two, three, four or five residues within a CDR region are altered.
Accordingly, in another embodiment, the invention provides isolated Factor P- binding antibodies, or antigen binding fragments thereof, consisting of a heavy chain variable region having a VH CDR1 region consisting of an amino acid sequence selected from the group having SEQ ID NOs: 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, and 267 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, or 267; a VH CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184,
198, 212, 226, 240, 254, and 268 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, or 268; a VH CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185,
199, 213, 227, 241 , 255, and 269, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs:
3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, or 269; a VL CDR1 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186,
200, 214, 228, 242, 256, and 270, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs:
4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, or 270; a VL CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 5, 19, 33, 47, 61 , 75, 89, 103, 1 17, 131 , 145, 159, 173, 187,
201 , 215, 229, 243, 257, and 271 , or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 5, 19, 33, 47, 61 , 75, 89, 103, 1 17, 131 , 145, 159, 173, 187, 201 , 215, 229, 243, 257, or 271 ; and a VL CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 1 18, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs:
6, 20, 34, 48, 62, 76, 90, 104, 1 18, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, or 272.
Accordingly, in another embodiment, the invention provides isolated Factor P- binding antibodies, or antigen binding fragments thereof, consisting of a heavy chain variable region having a VH CDR1 region consisting of an amino acid sequence selected from the group having SEQ ID NOs: 281 , 287, 293, 299, 305, 31 1 , 317, 323, 329, 335, 341 , 347, 353, 359, 365, 371 , 377, 383, 389, and 395 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 281 , 287, 293, 299, 305, 31 1 , 317, 323, 329, 335, 341 , 347, 353, 359, 365, 371 , 377, 383, 389, or 395; a VH CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, and 396 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, or 396; a VH CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 283, 289, 295, 301 , 307, 313, 319, 325, 331 , 337, 343, 349, 355, 361 , 367, 373, 379, 385, 391 , and 397, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 283, 289, 295, 301 , 307, 313, 319, 325, 331 , 337, 343, 349, 355, 361 , 367, 373, 379, 385, 391 , or 397; a VL CDR1 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374,
380, 386, 392, and 398, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, or 398; a VL CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 285, 291 , 297, 303, 309, 315, 321 , 327, 333, 339, 345, 351 , 357, 363, 369, 375, 381 , 387, 393, and 399, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 285, 291 , 297, 303, 309, 315, 321 , 327, 333, 339, 345, 351 , 357, 363, 369, 375,
381 , 387, 393, or 399; and a VL CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, and 400, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, or 400.
Grafting Antigen-binding Domains Into Alternative Frameworks or Scaffolds
A wide variety of antibody/ immunoglobulin frameworks or scaffolds can be employed so long as the resulting polypeptide includes at least one binding region which specifically binds to Factor P. Such frameworks or scaffolds include the 5 main idiotypes of human immunoglobulins, or fragments thereof, and include immunoglobulins of other animal species, preferably having humanized aspects. Single heavy-chain antibodies such as those identified in camelids are of particular interest in this regard. Novel frameworks, scaffolds and fragments continue to be discovered and developed by those skilled in the art.
In one aspect, the invention pertains to generating non-immunoglobulin based antibodies using non- immunoglobulin scaffolds onto which CDRs of the invention can be grafted. Known or future non-immunoglobulin frameworks and scaffolds may be employed, as long as they comprise a binding region specific for the target Factor P protein. Known non-immunoglobulin frameworks or scaffolds include, but are not limited to, fibronectin (Compound Therapeutics, Inc., Waltham, MA), ankyrin (Molecular Partners AG, Zurich, Switzerland), domain antibodies (Domantis, Ltd., Cambridge, MA, and Ablynx nv, Zwijnaarde, Belgium), lipocalin (Pieris Proteolab AG, Freising, Germany), small modular immuno-pharmaceuticals (Trubion Pharmaceuticals Inc., Seattle, WA), maxybodies (Avidia, Inc., Mountain View, CA), Protein A (Affibody AG, Sweden), and affilin (gamma-crystallin or ubiquitin) (Scil Proteins GmbH, Halle, Germany).
The fibronectin scaffolds are based on fibronectin type III domain (e.g., the tenth module of the fibronectin type III (10 Fn3 domain)). The fibronectin type III domain has 7 or 8 beta strands which are distributed between two beta sheets, which themselves pack against each other to form the core of the protein, and further containing loops
(analogous to CDRs) which connect the beta strands to each other and are solvent exposed. There are at least three such loops at each edge of the beta sheet sandwich, where the edge is the boundary of the protein perpendicular to the direction of the beta strands (see US 6,818,418). These fibronectin-based scaffolds are not an
immunoglobulin, although the overall fold is closely related to that of the smallest functional antibody fragment, the variable region of the heavy chain, which comprises the entire antigen recognition unit in camel and llama IgG. Because of this structure, the non-immunoglobulin antibody mimics antigen binding properties that are similar in nature and affinity to those of antibodies. These scaffolds can be used in a loop randomization and shuffling strategy in vitro that is similar to the process of affinity maturation of antibodies in vivo. These fibronectin-based molecules can be used as scaffolds where the loop regions of the molecule can be replaced with CDRs of the invention using standard cloning techniques.
The ankyrin technology is based on using proteins with ankyrin derived repeat modules as scaffolds for bearing variable regions which can be used for binding to different targets. The ankyrin repeat module is a 33 amino acid polypeptide consisting of two anti-parallel ohelices and a β-turn. Binding of the variable regions is mostly optimized by using ribosome display.
Avimers are derived from natural A-domain containing protein such as LRP-1 . These domains are used by nature for protein-protein interactions and in human over 250 proteins are structurally based on A-domains. Avimers consist of a number of different "A-domain" monomers (2-10) linked via amino acid linkers. Avimers can be created that can bind to the target antigen using the methodology described in, for example, U.S. Patent Application Publication Nos. 20040175756; 20050053973;
20050048512; and 20060008844.
Affibody affinity ligands are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold domain consists of 58 amino acids, 13 of which are randomized to generate affibody libraries with a large number of ligand variants (See e.g., US 5,831 ,012). Affibody molecules mimic antibodies, they have a molecular weight of 6 kDa, compared to the molecular weight of antibodies, which is 150 kDa. In spite of its small size, the binding site of affibody molecules is similar to that of an antibody.
Anticalins are products developed by the company Pieris ProteoLab AG. They are derived from lipocalins, a widespread group of small and robust proteins that are usually involved in the physiological transport or storage of chemically sensitive or insoluble compounds. Several natural lipocalins occur in human tissues or body liquids. The protein architecture is reminiscent of immunoglobulins, with hypervariable loops on top of a rigid framework. However, in contrast with antibodies or their recombinant fragments, lipocalins are composed of a single polypeptide chain with 160 to 180 amino acid residues, being just marginally bigger than a single immunoglobulin domain. The set of four loops, which makes up the binding pocket, shows pronounced structural plasticity and tolerates a variety of side chains. The binding site can thus be reshaped in a proprietary process in order to recognize prescribed target molecules of different shape with high affinity and specificity. One protein of lipocalin family, the bilin-binding protein (BBP) of Pieris Brassicae has been used to develop anticalins by mutagenizing the set of four loops. One example of a patent application describing anticalins is in PCT
Publication No. WO 199916873.
Affilin molecules are small non-immunoglobulin proteins which are designed for specific affinities towards proteins and small molecules. New affilin molecules can be very quickly selected from two libraries, each of which is based on a different human derived scaffold protein. Affilin molecules do not show any structural homology to immunoglobulin proteins. Currently, two affilin scaffolds are employed, one of which is gamma crystalline, a human structural eye lens protein and the other is "ubiquitin" superfamily proteins. Both human scaffolds are very small, show high temperature stability and are almost resistant to pH changes and denaturing agents. This high stability is mainly due to the expanded beta sheet structure of the proteins. Examples of gamma crystalline derived proteins are described in WO200104144 and examples of "ubiquitin-like" proteins are described in WO2004106368.
Protein epitope mimetics (PEM) are medium-sized, cyclic, peptide-like molecules (MW 1-2kDa) mimicking beta-hairpin secondary structures of proteins, the major secondary structure involved in protein-protein interactions.
The present invention provides fully human antibodies that specifically bind to a Factor P protein. Compared to the chimeric or humanized antibodies, the human Factor P-binding antibodies of the invention have further reduced antigenicity when
administered to human subjects.
Camelid antibodies
Antibody proteins obtained from members of the camel and dromedary (Camelus bactrianus and Calelus dromaderius) family including new world members such as llama species (Lama paccos, Lama glama and Lama vicugna) have been characterized with respect to size, structural complexity and antigenicity for human subjects. Certain IgG antibodies from this family of mammals as found in nature lack light chains, and are thus structurally distinct from the typical four chain quaternary structure having two heavy and two light chains, for antibodies from other animals. See PCT/EP93/02214 (WO 94/04678 published 3 March 1994).
A region of the camelid antibody which is the small single variable domain identified as VHH can be obtained by genetic engineering to yield a small protein having high affinity for a target, resulting in a low molecular weight antibody-derived protein known as a "camelid nanobody". See U.S. patent number 5,759,808 issued June 2, 1998; see also Stijlemans, B. et al., 2004 J Biol Chem 279: 1256-1261 ; Dumoulin, M. et a/., 2003 Nature 424: 783-788; Pleschberger, M. et al. 2003 Bioconjugate Chem 14: 440- 448; Cortez-Retamozo, V. et al. 2002 Int J Cancer 89: 456-62; and Lauwereys, M. et al. 1998 EMBO J 17: 3512-3520. Engineered libraries of camelid antibodies and antibody fragments are commercially available, for example, from Ablynx, Ghent, Belgium. As with other antibodies of non-human origin, an amino acid sequence of a camelid antibody can be altered recombinantly to obtain a sequence that more closely resembles a human sequence, i.e., the nanobody can be "humanized". Thus the natural low antigenicity of camelid antibodies to humans can be further reduced.
The camelid nanobody has a molecular weight approximately one-tenth that of a human IgG molecule, and the protein has a physical diameter of only a few nanometers. One consequence of the small size is the ability of camelid nanobodies to bind to antigenic sites that are functionally invisible to larger antibody proteins, i.e., camelid nanobodies are useful as reagents detect antigens that are otherwise cryptic using classical immunological techniques, and as possible therapeutic agents. Thus yet another consequence of small size is that a camelid nanobody can inhibit as a result of binding to a specific site in a groove or narrow cleft of a target protein, and hence can serve in a capacity that more closely resembles the function of a classical low molecular weight drug than that of a classical antibody.
The low molecular weight and compact size further result in camelid nanobodies being extremely thermostable, stable to extreme pH and to proteolytic digestion, and poorly antigenic. Another consequence is that camelid nanobodies readily move from the circulatory system into tissues, and even cross the blood-brain barrier and can treat disorders that affect nervous tissue. Nanobodies can further facilitated drug transport across the blood brain barrier. See U.S. patent application 20040161738 published August 19, 2004. These features combined with the low antigenicity to humans indicate great therapeutic potential. Further, these molecules can be fully expressed in prokaryotic cells such as E. coli and are expressed as fusion proteins with
bacteriophage and are functional. Accordingly, a feature of the present invention is a camelid antibody or nanobody having high affinity for Factor P. In certain embodiments herein, the camelid antibody or nanobody is naturally produced in the camelid animal, i.e., is produced by the camelid following immunization with Factor P or a peptide fragment thereof, using techniques described herein for other antibodies. Alternatively, the Factor P-binding camelid nanobody is engineered, i.e., produced by selection for example from a library of phage displaying appropriately mutagenized camelid nanobody proteins using panning procedures with Factor P as a target as described in the examples herein. Engineered nanobodies can further be customized by genetic engineering to have a half life in a recipient subject of from 45 minutes to two weeks. In a specific embodiment, the camelid antibody or nanobody is obtained by grafting the CDRs sequences of the heavy or light chain of the human antibodies of the invention into nanobody or single domain antibody framework sequences, as described for example in PCT/EP93/02214.
Bispecific Molecules and Multivalent Antibodies
In another aspect, the present invention features bispecific or multispecific molecules comprising a Factor P-binding antibody, or a fragment thereof, of the invention. An antibody of the invention, or antigen-binding regions thereof, can be derivatized or linked to another functional molecule, e.g., another peptide or protein (e.g., another antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules. The antibody of the invention may in fact be derivatized or linked to more than one other functional molecule to generate multi-specific molecules that bind to more than two different binding sites and/or target molecules; such multi-specific molecules are also intended to be encompassed by the term "bispecific molecule" as used herein. To create a bispecific molecule of the invention, an antibody of the invention can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, peptide or binding mimetic, such that a bispecific molecule results.
Accordingly, the present invention includes bispecific molecules comprising at least one first binding specificity for Factor P and a second binding specificity for a second target epitope. For example, the second target epitope is another epitope of Factor P different from the first target epitope. Additionally, for the invention in which the bispecific molecule is multi-specific, the molecule can further include a third binding specificity, in addition to the first and second target epitope.
In one embodiment, the bispecific molecules of the invention comprise as a binding specificity at least one antibody, or an antibody fragment thereof, including, e.g., a Fab, Fab', F(ab')2, Fv, or a single chain Fv. The antibody may also be a light chain or heavy chain dimer, or any minimal fragment thereof such as a Fv or a single chain construct as described in Ladner ef a/. U.S. Patent No. 4,946,778.
Diabodies are bivalent, bispecific molecules in which VH and VL domains are expressed on a single polypeptide chain, connected by a linker that is too short to allow for pairing between the two domains on the same chain. The VH and VL domains pair with complementary domains of another chain, thereby creating two antigen binding sites (see e.g., Holliger et ai, 1993 Proc. Natl. Acad. Sci. USA 90:6444-6448; Poljak ef a/., 1994 Structure 2:1 121 -1 123). Diabodies can be produced by expressing two
polypeptide chains with either the structure VHA-VLB and VHB-VLA (VH-VL
configuration), or VLA-VHB and VLB-VHA (VL-VH configuration) within the same cell. Most of them can be expressed in soluble form in bacteria. Single chain diabodies (scDb) are produced by connecting the two diabody-forming polypeptide chains with linker of approximately 15 amino acid residues (see Holliger and Winter, 1997 Cancer Immunol. Immunother., 45(3-4):128-30; Wu ef a/., 1996 Immunotechnology, 2(1 ):21 -36). scDb can be expressed in bacteria in soluble, active monomeric form (see Holliger and Winter, 1997 Cancer Immunol. Immunother., 45(34): 128-30; Wu et al., 1996
Immunotechnology, 2(1 ):21-36; Pluckthun and Pack, 1997 Immunotechnology, 3(2): 83- 105; Ridgway et al., 1996 Protein Eng., 9(7):617-21 ). A diabody can be fused to Fc to generate a "di-diabody" (see Lu et al., 2004 J. Biol. Chem., 279(4):2856-65).
Other antibodies which can be employed in the bispecific molecules of the invention are murine, chimeric and humanized monoclonal antibodies.
Bispecific molecules can be prepared by conjugating the constituent binding specificities, using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then conjugated to one another. When the binding specificities are proteins or peptides, a variety of coupling or cross- linking agents can be used for covalent conjugation. Examples of cross-linking agents include protein A, carbodiimide, N-succinimidyl-S-acetyl-thioacetate (SATA), 5,5'- dithiobis(2-nitrobenzoic acid) (DTNB), o-phenylenedimaleimide (oPDM), N-succinimidyl- 3-(2-pyridyldithio)propionate (SPDP), and sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohaxane-l-carboxylate (sulfo-SMCC) (see e.g., Karpovsky et al., 1984 J. Exp. Med. 160:1686; Liu, MA et al., 1985 Proc. Natl. Acad. Sci. USA 82:8648). Other methods include those described in Paulus, 1985 Behring Ins. Mitt. No. 78, 1 18-132; Brennan et al., 1985 Science 229:81 -83), and Glennie et al., 1987 J. Immunol. 139: 2367-2375). Conjugating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co. (Rockford, IL).
When the binding specificities are antibodies, they can be conjugated by sulfhydryl bonding of the C-terminus hinge regions of the two heavy chains. In a particularly embodiment, the hinge region is modified to contain an odd number of sulfhydryl residues, for example one, prior to conjugation.
Alternatively, both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell. This method is particularly useful where the bispecific molecule is a mAb x mAb, mAb x Fab, Fab x F(ab')2 or ligand x Fab fusion protein. A bispecific molecule of the invention can be a single chain molecule comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two binding determinants. Bispecific molecules may comprise at least two single chain molecules. Methods for preparing bispecific molecules are described for example in U.S. Patent Number 5,260,203; U.S. Patent Number 5,455,030; U.S. Patent Number 4,881 ,175; U.S. Patent Number 5, 132,405; U.S. Patent Number 5,091 ,513; U.S. Patent Number 5,476,786; U.S. Patent Number 5,013,653; U.S. Patent Number 5,258,498; and U.S. Patent Number 5,482,858.
Binding of the bispecific molecules to their specific targets can be confirmed by, for example, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (REA), FACS analysis, bioassay (e.g., growth inhibition), or Western Blot assay. Each of these assays generally detects the presence of protein-antibody complexes of particular interest by employing a labeled reagent (e.g., an antibody) specific for the complex of interest.
In another aspect, the present invention provides multivalent compounds comprising at least two identical or different antigen-binding portions of the antibodies of the invention binding to Factor P. The antigen-binding portions can be linked together via protein fusion or covalent or non covalent linkage. Alternatively, methods of linkage have been described for the bispecfic molecules. Tetravalent compounds can be obtained for example by cross-linking antibodies of the antibodies of the invention with an antibody that binds to the constant regions of the antibodies of the invention, for example the Fc or hinge region.
Trimerizing domain are described for example in Borean patent EP 1 012 280B1. Pentamerizing modules are described for example in PCT/EP97/05897.
Antibodies with Extended Half Life
The present invention provides for antibodies that specifically bind to Factor P protein which have an extended half-life in vivo.
Many factors may affect a protein's half life in vivo. For examples, kidney filtration, metabolism in the liver, degradation by proteolytic enzymes (proteases), and immunogenic responses (e.g., protein neutralization by antibodies and uptake by macrophages and dentritic cells). A variety of strategies can be used to extend the half life of the antibodies of the present invention. For example, by chemical linkage to polyethyleneglycol (PEG), reCODE PEG, antibody scaffold, polysialic acid (PSA), hydroxyethyl starch (HES), albumin-binding ligands, and carbohydrate shields; by genetic fusion to proteins binding to serum proteins, such as albumin, IgG, FcRn, and transferring; by coupling (genetically or chemically) to other binding moieties that bind to serum proteins, such as nanoboies, Fabs, DARPins, avimers, affibodies, and anticalins; by genetic fusion to rPEG, albumin, domain of albumin, albumin-binding proteins, and Fc; or by incorporation into nancarriers, slow release formulations, or medical devices.
To prolong the serum circulation of antibodies in vivo, inert polymer molecules such as high molecular weight PEG can be attached to the antibodies or a fragment thereof with or without a multifunctional linker either through site-specific conjugation of the PEG to the N- or C-terminus of the antibodies or via epsilon-amino groups present on lysine residues. To pegylate an antibody, the antibody, or fragment thereof, typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment. The pegylation can be carried out by an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer). As used herein, the term "polyethylene glycol" is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (C1 -C10) alkoxy- or aryloxy-polyethylene glycol or polyethylene glycol- maleimide. In certain embodiments, the antibody to be pegylated is an aglycosylated antibody. Linear or branched polymer derivatization that results in minimal loss of biological activity will be used. The degree of conjugation can be closely monitored by SDS-PAGE and mass spectrometry to ensure proper conjugation of PEG molecules to the antibodies. Unreacted PEG can be separated from antibody-PEG conjugates by size-exclusion or by ion-exchange chromatography. PEG-derivatized antibodies can be tested for binding activity as well as for in vivo efficacy using methods well-known to those of skill in the art, for example, by immunoassays described herein. Methods for pegylating proteins are known in the art and can be applied to the antibodies of the invention. See for example, EP 0 154 316 by Nishimura et al. and EP 0 401 384 by Ishikawa et al.
Other modified pegylation technologies include reconstituting chemically orthogonal directed engineering technology (ReCODE PEG), which incorporates chemically specified side chains into biosynthetic proteins via a reconstituted system that includes tRNA synthetase and tRNA. This technology enables incorporation of more than 30 new amino acids into biosynthetic proteins in E.coli, yeast, and mammalian cells. The tRNA incorporates a nonnative amino acid any place an amber codon is positioned, converting the amber from a stop codon to one that signals incorporation of the chemically specified amino acid.
Recombinant pegylation technology (rPEG) can also be used for serum halflife extension. This technology involves genetically fusing a 300-600 amino acid
unstructured protein tail to an existing pharmaceutical protein. Because the apparent molecular weight of such an unstructured protein chain is about 15-fold larger than its actual molecular weight, the serum halflife of the protein is greatly increased. In contrast to traditional PEGylation, which requires chemical conjugation and repurification, the manufacturing process is greatly simplified and the product is homogeneous.
Polysialytion is another technology, which uses the natural polymer polysialic acid (PSA) to prolong the active life and improve the stability of therapeutic peptides and proteins. PSA is a polymer of sialic acid (a sugar). When used for protein and therapeutic peptide drug delivery, polysialic acid provides a protective microenvironment on conjugation. This increases the active life of the therapeutic protein in the circulation and prevents it from being recognized by the immune system. The PSA polymer is naturally found in the human body. It was adopted by certain bacteria which evolved over millions of years to coat their walls with it. These naturally polysialylated bacteria were then able, by virtue of molecular mimicry, to foil the body's defense system. PSA, nature's ultimate stealth technology, can be easily produced from such bacteria in large quantities and with predetermined physical characteristics. Bacterial PSA is completely non-immunogenic, even when coupled to proteins, as it is chemically identical to PSA in the human body.
Another technology includes the use of hydroxyethyl starch ("HES") derivatives linked to antibodies. HES is a modified natural polymer derived from waxy maize starch and can be metabolized by the body's enzymes. HES solutions are usually administered to substitute deficient blood volume and to improve the rheological properties of the blood. Hesylation of an antibody enables the prolongation of the circulation half-life by increasing the stability of the molecule, as well as by reducing renal clearance, resulting in an increased biological activity. By varying different parameters, such as the molecular weight of HES, a wide range of HES antibody conjugates can be customized.
Antibodies having an increased half-life in vivo can also be generated introducing one or more amino acid modifications (i.e., substitutions, insertions or deletions) into an IgG constant domain, or FcRn binding fragment thereof (preferably a Fc or hinge Fc domain fragment). See, e.g., International Publication No. WO 98/23289; International Publication No. WO 97/34631 ; and U.S. Patent No. 6,277,375.
Further, antibodies can be conjugated to albumin (e.g., human serum albumin; HSA) in order to make the antibody or antibody fragment more stable in vivo or have a longer half life in vivo. The techniques are well-known in the art, see, e.g., International Publication Nos. WO 93/15199, WO 93/15200, and WO 01/77137; and European Patent No. EP 413,622. In addition, in the context of a bispecific antibody as described above, the specificities of the antibody can be designed such that one binding domain of the antibody binds to Factor P while a second binding domain of the antibody binds to serum albumin, preferably HSA.
The strategies for increasing half life is especially useful in nanobodies, fibronectin-based binders, and other antibodies or proteins for which increased in vivo half life is desired.
Antibody Conjugates
The present invention provides antibodies or fragments thereof that specifically bind to a Factor P protein recombinantly fused or chemically conjugated (including both covalent and non-covalent conjugations) to a heterologous protein or polypeptide (or fragment thereof, preferably to a polypeptide of at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90 or at least 100 amino acids) to generate fusion proteins. In particular, the invention provides fusion proteins comprising an antigen-binding fragment of an antibody described herein {e.g., a Fab fragment, Fd fragment, Fv fragment, F(ab)2 fragment, a VH domain, a VH CDR, a VL domain or a VL CDR) and a heterologous protein, polypeptide, or peptide. Methods for fusing or conjugating proteins, polypeptides, or peptides to an antibody or an antibody fragment are known in the art. See, e.g., U.S. Patent Nos. 5,336,603, 5,622,929, 5,359,046, 5,349,053, 5,447,851 , and 5, 1 12,946; European Patent Nos. EP 307,434 and EP 367,166; International Publication Nos. WO 96/04388 and WO 91/06570; Ashkenazi et al., 1991 , Proc. Natl. Acad. Sci. USA 88: 10535-10539; Zheng et ai, 1995, J.
Immunol. 154:5590-5600; and Vil et al., 1992, Proc. Natl. Acad. Sci. USA 89:1 1337- 1 1341 .
Additional fusion proteins may be generated through the techniques of gene- shuffling, motif-shuffling, exon-shuffling, and/or codon-shuffling (collectively referred to as "DNA shuffling"). DNA shuffling may be employed to alter the activities of antibodies of the invention or fragments thereof (e.g., antibodies or fragments thereof with higher affinities and lower dissociation rates). See, generally, U.S. Patent Nos. 5,605,793, 5,81 1 ,238, 5,830,721 , 5,834,252, and 5,837,458; Patten et al., 1997, Curr. Opinion Biotechnol. 8:724-33; Harayama, 1998, Trends Biotechnol. 16(2):76-82; Hansson, et al., 1999, J. Mol. Biol. 287:265-76; and Lorenzo and Blasco, 1998, Biotechniques 24(2):308- 313 (each of these patents and publications are hereby incorporated by reference in its entirety). Antibodies or fragments thereof, or the encoded antibodies or fragments thereof, may be altered by being subjected to random mutagenesis by error-prone PCR, random nucleotide insertion or other methods prior to recombination. A polynucleotide encoding an antibody or fragment thereof that specifically binds to a Factor P protein may be recombined with one or more components, motifs, sections, parts, domains, fragments, etc. of one or more heterologous molecules.
Moreover, the antibodies or fragments thereof can be fused to marker sequences, such as a peptide to facilitate purification. In preferred embodiments, the marker amino acid sequence is a hexa-histidine peptide, such as the tag provided in a pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 9131 1 ), among others, many of which are commercially available. As described in Gentz et al., 1989, Proc. Natl. Acad. Sci. USA 86:821-824, for instance, hexa-histidine provides for convenient purification of the fusion protein. Other peptide tags useful for purification include, but are not limited to, the hemagglutinin ("HA") tag, which corresponds to an epitope derived from the influenza hemagglutinin protein (Wilson et al., 1984, Cell 37:767), and the "flag" tag. In other embodiments, antibodies of the present invention or fragments thereof conjugated to a diagnostic or detectable agent. Such antibodies can be useful for monitoring or prognosing the onset, development, progression and/or severity of a disease or disorder as part of a clinical testing procedure, such as determining the efficacy of a particular therapy. Such diagnosis and detection can accomplished by coupling the antibody to detectable substances including, but not limited to, various enzymes, such as, but not limited to, horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase; prosthetic groups, such as, but not limited to, streptavidinlbiotin and avidin/biotin; fluorescent materials, such as, but not limited to, umbelliferone, fluorescein, fluorescein isothiocynate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; luminescent materials, such as, but not limited to, luminol; bioluminescent materials, such as but not limited to, luciferase, luciferin, and aequorin; radioactive materials, such as, but not limited to, iodine (131 1, 1251, 1231, and 121 1,), carbon (14C), sulfur (35S), tritium (3H), indium (1 15ln, 1 13ln, 1 12ln, and 1 1 1 1n,), technetium (99Tc), thallium (201ΤΊ), gallium (68Ga, 67Ga), palladium (103Pd), molybdenum (99Mo), xenon (133Xe), fluorine (18F), 153Sm, 177Lu, 159Gd, 149Pm, 140La, 175Yb, 166Ho, 90Y, 47Sc, 186Re, 188Re,142 Pr, 105Rh, 97Ru, 68Ge, 57Co, 65Zn, 85Sr, 32P, 153Gd, 169Yb, 51 Cr, 54Mn, 75Se, 1 13Sn, and 1 17Tin; and positron emitting metals using various positron emission tomographies, and
noradioactive paramagnetic metal ions.
The present invention further encompasses uses of antibodies or fragments thereof conjugated to a therapeutic moiety. An antibody or fragment thereof may be conjugated to a therapeutic moiety such as a cytotoxin, e.g., a cytostatic or cytocidal agent, a therapeutic agent or a radioactive metal ion, e.g., alpha-emitters. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells.
Further, an antibody or fragment thereof may be conjugated to a therapeutic moiety or drug moiety that modifies a given biological response. Therapeutic moieties or drug moieties are not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein, peptide, or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, cholera toxin, or diphtheria toxin; a protein such as tumor necrosis factor, ointerferon, β-interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator, an apoptotic agent, an anti-angiogenic agent; or, a biological response modifier such as, for example, a lymphokine. Moreover, an antibody can be conjugated to therapeutic moieties such as a radioactive metal ion, such as alph-emiters such as 213Bi or macrocyclic chelators useful for conjugating radiometal ions, including but not limited to, 131 In, 131 LU, 131Y, 131 Ho, 131 Sm, to polypeptides. In certain embodiments, the macrocyclic chelator is 1 ,4,7, 10-tetraazacyclododecane-N,N',N",N"'-tetraacetic acid (DOTA) which can be attached to the antibody via a linker molecule. Such linker molecules are commonly known in the art and described in Denardo et al., 1998, Clin Cancer Res. 4(10):2483-90; Peterson et al., 1999, Bioconjug. Chem. 10(4):553-7; and Zimmerman et al., 1999, NucI. Med. Biol. 26(8):943-50, each incorporated by reference in their entireties.
Techniques for conjugating therapeutic moieties to antibodies are well known, see, e.g., Arnon et al., "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy", in Monoclonal Antibodies And Cancer Therapy, Reisfeld et al. (eds.), pp. 243- 56 (Alan R. Liss, Inc. 1985); Hellstrom et al., "Antibodies For Drug Delivery", in
Controlled Drug Delivery (2nd Ed.), Robinson et al. (eds.), pp. 623-53 (Marcel Dekker, Inc. 1987); Thorpe, "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review", in Monoclonal Antibodies 84: Biological And Clinical Applications, Pinchera et al. (eds.), pp. 475-506 (1985); "Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy", in Monoclonal
Antibodies For Cancer Detection And Therapy, Baldwin et al. (eds.), pp. 303-16
(Academic Press 1985), and Thorpe et al., 1982, Immunol. Rev. 62:1 19-58.
Antibodies may also be attached to solid supports, which are particularly useful for immunoassays or purification of the target antigen. Such solid supports include, but are not limited to, glass, cellulose, polyacrylamide, nylon, polystyrene, polyvinyl chloride or polypropylene.
Methods of Producing Antibodies of the Invention
Nucleic Acids Encoding the Antibodies
The invention provides substantially purified nucleic acid molecules which encode polypeptides comprising segments or domains of the Factor P -binding antibody chains described above. Some of the nucleic acids of the invention comprise the nucleotide sequence encoding the heavy chain variable region shown in SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259 or 273, and/or the nucleotide sequence encoding the light chain variable region shown in SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274. In a specific embodiment, the nucleic acid molecules are those identified in Table 1 . Some other nucleic acid molecules of the invention comprise nucleotide sequences that are substantially identical {e.g., at least 65, 80%, 95%, or 99%) to the nucleotide sequences of those identified in Table 1. When expressed from appropriate expression vectors, polypeptides encoded by these polynucleotides are capable of exhibiting Factor P antigen binding capacity.
Also provided in the invention are polynucleotides which encode at least one CDR region and usually all three CDR regions from the heavy or light chain of the Factor P-binding antibody set forth above. Some other polynucleotides encode all or substantially all of the variable region sequence of the heavy chain and/or the light chain of the Factor P-binding antibody set forth above. Because of the degeneracy of the code, a variety of nucleic acid sequences will encode each of the immunoglobulin amino acid sequences.
The nucleic acid molecules of the invention can encode both a variable region and a constant region of the antibody. Some of nucleic acid sequences of the invention comprise nucleotides encoding a mature heavy chain sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature heavy chain sequence set forth in SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275. Some other nucleic acid sequences comprising nucleotide encoding a mature light chain sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature light chain sequence set forth in SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276.
The polynucleotide sequences can be produced by de novo solid-phase DNA synthesis or by PCR mutagenesis of an existing sequence (e.g., sequences as described in the Examples below) encoding a Factor P-binding antibody or its binding fragment. Direct chemical synthesis of nucleic acids can be accomplished by methods known in the art, such as the phosphotriester method of Narang et al., 1979, Meth. Enzymol. 68:90; the phosphodiester method of Brown et al., Meth. Enzymol. 68:109, 1979; the
diethylphosphoramidite method of Beaucage et al., Tetra. Lett., 22:1859, 1981 ; and the solid support method of U.S. Patent No. 4,458,066. Introducing mutations to a polynucleotide sequence by PCR can be performed as described in, e.g., PCR
Technology: Principles and Applications for DNA Amplification, H.A. Erlich (Ed.), Freeman Press, NY, NY, 1992; PCR Protocols: A Guide to Methods and Applications, Innis et al. (Ed.), Academic Press, San Diego, CA, 1990; Mattila et al., Nucleic Acids Res. 19:967, 1991 ; and Eckert et al., PCR Methods and Applications 1 :17, 1991 . Also provided in the invention are expression vectors and host cells for producing the Factor P-binding antibodies described above. Various expression vectors can be employed to express the polynucleotides encoding the Factor P-binding antibody chains or binding fragments. Both viral-based and nonviral expression vectors can be used to produce the antibodies in a mammalian host cell. Nonviral vectors and systems include plasmids, episomal vectors, typically with an expression cassette for expressing a protein or RNA, and human artificial chromosomes (see, e.g., Harrington et al., Nat Genet 15:345, 1997). For example, nonviral vectors useful for expression of the Factor P- binding polynucleotides and polypeptides in mammalian (e.g., human) cells include pThioHis A, B & C, pcDNA3.1/His, pEBVHis A, B & C, (Invitrogen, San Diego, CA), MPSV vectors, and numerous other vectors known in the art for expressing other proteins. Useful viral vectors include vectors based on retroviruses, adenoviruses, adenoassociated viruses, herpes viruses, vectors based on SV40, papilloma virus, HBP Epstein Barr virus, vaccinia virus vectors and Semliki Forest virus (SFV). See, Brent et al., supra; Smith, Annu. Rev. Microbiol. 49:807, 1995; and Rosenfeld et al., Cell 68:143, 1992.
The choice of expression vector depends on the intended host cells in which the vector is to be expressed. Typically, the expression vectors contain a promoter and other regulatory sequences (e.g., enhancers) that are operably linked to the
polynucleotides encoding a Factor P-binding antibody chain or fragment. In some embodiments, an inducible promoter is employed to prevent expression of inserted sequences except under inducing conditions. Inducible promoters include, e.g., arabinose, lacZ, metallothionein promoter or a heat shock promoter. Cultures of transformed organisms can be expanded under noninducing conditions without biasing the population for coding sequences whose expression products are better tolerated by the host cells. In addition to promoters, other regulatory elements may also be required or desired for efficient expression of a Factor P-binding antibody chain or fragment. These elements typically include an ATG initiation codon and adjacent ribosome binding site or other sequences. In addition, the efficiency of expression may be enhanced by the inclusion of enhancers appropriate to the cell system in use (see, e.g., Scharf et al., Results Probl. Cell Differ. 20:125, 1994; and Bittner et al., Meth. Enzymol., 153:516, 1987). For example, the SV40 enhancer or CMV enhancer may be used to increase expression in mammalian host cells.
The expression vectors may also provide a secretion signal sequence position to form a fusion protein with polypeptides encoded by inserted Factor P-binding antibody sequences. More often, the inserted Factor P-binding antibody sequences are linked to a signal sequences before inclusion in the vector. Vectors to be used to receive sequences encoding Factor P-binding antibody light and heavy chain variable domains sometimes also encode constant regions or parts thereof. Such vectors allow expression of the variable regions as fusion proteins with the constant regions thereby leading to production of intact antibodies or fragments thereof. Typically, such constant regions are human.
The host cells for harboring and expressing the Factor P-binding antibody chains can be either prokaryotic or eukaryotic. E. coli is one prokaryotic host useful for cloning and expressing the polynucleotides of the present invention. Other microbial hosts suitable for use include bacilli, such as Bacillus subtilis, and other enterobacteriaceae, such as Salmonella, Serratia, and various Pseudomonas species. In these prokaryotic hosts, one can also make expression vectors, which typically contain expression control sequences compatible with the host cell (e.g., an origin of replication). In addition, any number of a variety of well-known promoters will be present, such as the lactose promoter system, a tryptophan (trp) promoter system, a beta-lactamase promoter system, or a promoter system from phage lambda. The promoters typically control expression, optionally with an operator sequence, and have ribosome binding site sequences and the like, for initiating and completing transcription and translation. Other microbes, such as yeast, can also be employed to express Factor P-binding polypeptides of the invention. Insect cells in combination with baculovirus vectors can also be used.
In some preferred embodiments, mammalian host cells are used to express and produce the Factor P -binding polypeptides of the present invention. For example, they can be either a hybridoma cell line expressing endogenous immunoglobulin genes (e.g., the 1 D6.C9 myeloma hybridoma clone as described in the Examples) or a mammalian cell line harboring an exogenous expression vector (e.g., the SP2/0 myeloma cells exemplified below). These include any normal mortal or normal or abnormal immortal animal or human cell. For example, a number of suitable host cell lines capable of secreting intact immunoglobulins have been developed including the CHO cell lines, various Cos cell lines, HeLa cells, myeloma cell lines, transformed B-cells and hybridomas. The use of mammalian tissue cell culture to express polypeptides is discussed generally in, e.g., Winnacker, FROM GENES TO CLONES, VCH Publishers, N.Y., N.Y., 1987. Expression vectors for mammalian host cells can include expression control sequences, such as an origin of replication, a promoter, and an enhancer (see, e.g., Queen, et al., Immunol. Rev. 89:49-68, 1986), and necessary processing information sites, such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcriptional terminator sequences. These expression vectors usually contain promoters derived from mammalian genes or from mammalian viruses. Suitable promoters may be constitutive, cell type-specific, stage-specific, and/or modulatable or regulatable. Useful promoters include, but are not limited to, the metallothionein promoter, the constitutive adenovirus major late promoter, the dexamethasone-inducible MMTV promoter, the SV40 promoter, the MRP pollll promoter, the constitutive MPSV promoter, the tetracycline-inducible CMV promoter (such as the human immediate-early CMV promoter), the constitutive CMV promoter, and promoter-enhancer combinations known in the art.
Methods for introducing expression vectors containing the polynucleotide sequences of interest vary depending on the type of cellular host. For example, calcium chloride transfection is commonly utilized for prokaryotic cells, whereas calcium phosphate treatment or electroporation may be used for other cellular hosts. (See generally Sambrook, et al., supra). Other methods include, e.g., electroporation, calcium phosphate treatment, liposome-mediated transformation, injection and microinjection, ballistic methods, virosomes, immunoliposomes, polycation:nucleic acid conjugates, naked DNA, artificial virions, fusion to the herpes virus structural protein VP22 (Elliot and O'Hare, Cell 88:223, 1997), agent-enhanced uptake of DNA, and ex vivo transduction. For long-term, high-yield production of recombinant proteins, stable expression will often be desired. For example, cell lines which stably express Factor P -binding antibody chains or binding fragments can be prepared using expression vectors of the invention which contain viral origins of replication or endogenous expression elements and a selectable marker gene. Following the introduction of the vector, cells may be allowed to grow for 1-2 days in an enriched media before they are switched to selective media. The purpose of the selectable marker is to confer resistance to selection, and its presence allows growth of cells which successfully express the introduced sequences in selective media. Resistant, stably transfected cells can be proliferated using tissue culture techniques appropriate to the cell type.
Generation of monoclonal antibodies of the invention
Monoclonal antibodies (mAbs) can be produced by a variety of techniques, including conventional monoclonal antibody methodology e.g., the standard somatic cell hybridization technique of Kohler and Milstein, 1975 Nature 256: 495. Many techniques for producing monoclonal antibody can be employed e.g., viral or oncogenic
transformation of B lymphocytes. An animal systems for preparing hybridomas include the murine, rat and rabbit systems. Hybridoma production in the mouse is a well established procedure.
Immunization protocols and techniques for isolation of immunized splenocytes for fusion are known in the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known.
Chimeric or humanized antibodies of the present invention can be prepared based on the sequence of a murine monoclonal antibody prepared as described above. DNA encoding the heavy and light chain immunoglobulins can be obtained from the murine hybridoma of interest and engineered to contain non-murine (e.g.,. human) immunoglobulin sequences using standard molecular biology techniques. For example, to create a chimeric antibody, the murine variable regions can be linked to human constant regions using methods known in the art (see e.g., U.S. Patent No. 4,816,567 to Cabilly et al.). To create a humanized antibody, the murine CDR regions can be inserted into a human framework using methods known in the art. See e.g., U.S. Patent No. 5225539 to Winter, and U.S. Patent Nos. 5530101 ; 5585089; 5693762 and 6180370 to Queen et al.
In a certain embodiment, the antibodies of the invention are human monoclonal antibodies. Such human monoclonal antibodies directed against Factor P can be generated using transgenic or transchromosomic mice carrying parts of the human immune system rather than the mouse system. These transgenic and transchromosomic mice include mice referred to herein as HuMAb mice and KM mice, respectively, and are collectively referred to herein as "human Ig mice."
The HuMAb mouse® (Medarex, Inc.) contains human immunoglobulin gene miniloci that encode un-rearranged human heavy (μ and γ) and κ light chain
immunoglobulin sequences, together with targeted mutations that inactivate the endogenous μ and κ chain loci (see e.g., Lonberg, et al., 1994 Nature 368(6474): 856- 859). Accordingly, the mice exhibit reduced expression of mouse IgM or κ, and in response to immunization, the introduced human heavy and light chain transgenes undergo class switching and somatic mutation to generate high affinity human IgGK monoclonal (Lonberg, N. et al., 1994 supra; reviewed in Lonberg, N., 1994 Handbook of Experimental Pharmacology 1 13:49-101 ; Lonberg, N. and Huszar, D., 1995 Intern. Rev. Immunol.13: 65-93, and Harding, F. and Lonberg, N., 1995 Ann. N. Y. Acad. Sci.
764:536-546). The preparation and use of HuMAb mice, and the genomic modifications carried by such mice, is further described in Taylor, L. et al., 1992 Nucleic Acids
Research 20:6287-6295; Chen, J. et at., 1993 International Immunology 5: 647-656; Tuaillon et al., 1993 Proc. Natl. Acad. Sci. USA 94:3720-3724; Choi et a/., 1993 Nature Genetics 4:1 17-123; Chen, J. et al., 1993 EMBO J. 12: 821 -830; Tuaillon et al., 1994 J. Immunol. 152:2912-2920; Taylor, L. et al., 1994 International Immunology 579-591 ; and Fishwild, D. et al., 1996 Nature Biotechnology 14: 845-851 , the contents of all of which are hereby specifically incorporated by reference in their entirety. See further, U.S. Patent Nos. 5,545,806; 5,569,825; 5,625, 126; 5,633,425; 5,789,650; 5,877,397;
5,661 ,016; 5,814,318; 5,874,299; and 5,770,429; all to Lonberg and Kay; U.S. Patent No. 5,545,807 to Surani et al.; PCT Publication Nos. WO 92103918, WO 93/12227, WO 94/25585, WO 971 13852, WO 98/24884 and WO 99/45962, all to Lonberg and Kay; and PCT Publication No. WO 01/14424 to Korman et al.
In another embodiment, human antibodies of the invention can be raised using a mouse that carries human immunoglobulin sequences on transgenes and
transchomosomes such as a mouse that carries a human heavy chain transgene and a human light chain transchromosome. Such mice, referred to herein as "KM mice", are described in detail in PCT Publication WO 02/43478 to Ishida et al.
Still further, alternative transgenic animal systems expressing human
immunoglobulin genes are available in the art and can be used to raise Factor P -binding antibodies of the invention. For example, an alternative transgenic system referred to as the Xenomouse (Abgenix, Inc.) can be used. Such mice are described in, e.g., U.S. Patent Nos. 5,939,598; 6,075, 181 ; 6, 1 14,598; 6, 150,584 and 6, 162,963 to Kucherlapati et al.
Moreover, alternative transchromosomic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise Factor P -binding antibodies of the invention. For example, mice carrying both a human heavy chain transchromosome and a human light chain tranchromosome, referred to as "TC mice" can be used; such mice are described in Tomizuka et al., 2000 Proc. Natl. Acad. Sci. USA 97:722-727. Furthermore, cows carrying human heavy and light chain
transchromosomes have been described in the art (Kuroiwa et al., 2002 Nature
Biotechnology 20:889-894) and can be used to raise Factor P -binding antibodies of the invention.
Human monoclonal antibodies of the invention can also be prepared using phage display methods for screening libraries of human immunoglobulin genes. Such phage display methods for isolating human antibodies are established in the art or described in the examples below. See for example: U.S. Patent Nos. 5,223,409; 5,403,484; and 5,571 ,698 to Ladner et a/.; U.S. Patent Nos. 5,427,908 and 5,580,717 to Dower et a/.; U.S. Patent Nos. 5,969, 108 and 6, 172, 197 to McCafferty et al.; and U.S. Patent Nos. 5,885,793; 6,521 ,404; 6,544,731 ; 6,555,313; 6,582,915 and 6,593,081 to Griffiths et al.
Human monoclonal antibodies of the invention can also be prepared using SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization. Such mice are described in, for example, U.S. Patent Nos. 5,476,996 and 5,698,767 to Wilson et al.
Framework or Fc engineering
Engineered antibodies of the invention include those in which modifications have been made to framework residues within VH and/or VL, e.g. to improve the properties of the antibody. Typically such framework modifications are made to decrease the immunogenicity of the antibody. For example, one approach is to "backmutate" one or more framework residues to the corresponding germline sequence. More specifically, an antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequences to the germline sequences from which the antibody is derived. To return the framework region sequences to their germline configuration, the somatic mutations can be "backmutated" to the germline sequence by, for example, site-directed mutagenesis. Such
"backmutated" antibodies are also intended to be encompassed by the invention.
Another type of framework modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell - epitopes to thereby reduce the potential immunogenicity of the antibody. This approach is also referred to as "deimmunization" and is described in further detail in U.S. Patent Publication No. 20030153043 by Can et al.
In addition or alternative to modifications made within the framework or CDR regions, antibodies of the invention may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity. Furthermore, an antibody of the invention may be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the antibody. Each of these embodiments is described in further detail below. The numbering of residues in the Fc region is that of the EU index of Kabat. In one embodiment, the hinge region of CH1 is modified such that the number of cysteine residues in the hinge region is altered, e.g., increased or decreased. This approach is described further in U.S. Patent No. 5,677,425 by Bodmer et al. The number of cysteine residues in the hinge region of CH1 is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.
In another embodiment, the Fc hinge region of an antibody is mutated to decrease the biological half-life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc-hinge fragment such that the antibody has impaired Staphylococcyl protein A (SpA) binding relative to native Fc-hinge domain SpA binding. This approach is described in further detail in U.S. Patent No. 6, 165,745 by Ward et al.
In another embodiment, the antibody is modified to increase its biological half-life. Various approaches are possible. For example, one or more of the following mutations can be introduced: T252L, T254S, T256F, as described in U.S. Patent No. 6,277,375 to Ward. Alternatively, to increase the biological half life, the antibody can be altered within the CH1 or CL region to contain a salvage receptor binding epitope taken from two loops of a CH2 domain of an Fc region of an IgG, as described in U.S. Patent Nos. 5,869,046 and 6, 121 ,022 by Presta et al.
In yet other embodiments, the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector functions of the antibody. For example, one or more amino acids can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but retains the antigen-binding ability of the parent antibody. The effector ligand to which affinity is altered can be, for example, an Fc receptor or the C1 component of
complement. This approach is described in further detail in U.S. Patent Nos. 5,624,821 and 5,648,260, both by Winter et al.
In another embodiment, one or more amino acids selected from amino acid residues can be replaced with a different amino acid residue such that the antibody has altered C1 q binding and/or reduced or abolished complement dependent cytotoxicity (CDC). This approach is described in further detail in U.S. Patent Nos. 6, 194,551 by Idusogie et al. In another embodiment, one or more amino acid residues are altered to thereby alter the ability of the antibody to fix complement. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al.
In yet another embodiment, the Fc region is modified to increase the ability of the antibody to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fey receptor by modifying one or more amino acids. This approach is described further in PCT Publication WO 00/42072 by Presta.
Moreover, the binding sites on human lgG1 for FcyRI, FcyRII, FcyRIII and FcRn have been mapped and variants with improved binding have been described (see Shields, R.L. et al., 2001 J. Biol. Chen. 276:6591-6604).
In still another embodiment, the glycosylation of an antibody is modified. For example, an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation). Glycosylation can be altered to, for example, increase the affinity of the antibody for "antigen'. Such carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence. For example, one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site. Such aglycosylation may increase the affinity of the antibody for antigen. Such an approach is described in further detail in U.S. Patent Nos. 5,714,350 and 6,350,861 by Co et al.
Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GlcNac structures. Such altered glycosylation patterns have been demonstrated to increase the ADCC ability of antibodies. Such carbohydrate modifications can be accomplished by, for example, expressing the antibody in a host cell with altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of the invention to thereby produce an antibody with altered glycosylation. For example, EP 1 , 176, 195 by Hang et al. describes a cell line with a functionally disrupted FUT8 gene, which encodes a fucosyl transferase, such that antibodies expressed in such a cell line exhibit hypofucosylation. PCT
Publication WO 03/035835 by Presta describes a variant CHO cell line, Lecl3 cells, with reduced ability to attach fucose to Asn(297)-linked carbohydrates, also resulting in hypofucosylation of antibodies expressed in that host cell (see also Shields, R.L. et al., 2002 J. Biol. Chem. 277:26733-26740). PCT Publication WO 99/54342 by Umana et al. describes cell lines engineered to express glycoprotein-modifying glycosyl transferases (e.g., beta(1 ,4)-N acetylglucosaminyltransferase III (GnTIII)) such that antibodies expressed in the engineered cell lines exhibit increased bisecting GlcNac structures which results in increased ADCC activity of the antibodies (see also Umana et al., 1999 Nat. Biotech. 17:176-180).
Methods of Engineering Altered Antibodies
As discussed above, the Factor P -binding antibodies having VH and VL sequences or full length heavy and light chain sequences shown herein can be used to create new Factor P-binding antibodies by modifying full length heavy chain and/or light chain sequences, VH and/or VL sequences, or the constant region(s) attached thereto. Thus, in another aspect of the invention, the structural features of a Factor P-binding antibody of the invention are used to create structurally related Factor P-binding antibodies that retain at least one functional property of the antibodies of the invention, such as binding to human Factor P and also inhibiting one or more functional properties of Factor P (e.g., inhibiting MAC deposition in a MAC deposition assay, inhibit red blood cell lysis in a hemolytic assay).
For example, one or more CDR regions of the antibodies of the present invention, or mutations thereof, can be combined recombinantly with known framework regions and/or other CDRs to create additional, recombinantly-engineered, Factor P - binding antibodies of the invention, as discussed above. Other types of modifications include those described in the previous section. The starting material for the engineering method is one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. To create the engineered antibody, it is not necessary to actually prepare (i.e., express as a protein) an antibody having one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. Rather, the information contained in the sequence(s) is used as the starting material to create a "second generation" sequence(s) derived from the original sequence(s) and then the "second generation" sequence(s) is prepared and expressed as a protein.
Accordingly, in another embodiment, the invention provides a method for preparing a Factor P-binding antibody consisting of a heavy chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 1 , 15, 29, 43, 57, 71 , 85, 99, 1 13, 127, 141 , 155, 169, 183, 197, 21 1 , 225, 239, 253, and 267, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 1 14, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 3, 17, 31 , 45, 59, 73, 87, 101 , 1 15, 129, 143, 157, 171 , 185, 199, 213, 227, 241 , 255, and 269; and a light chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 1 16, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 5, 19, 33, 47, 61 , 75, 89, 103, 1 17, 131 , 145, 159, 173,
187, 201 , 215, 229, 243, 257, and 271 , and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 1 18, 132, 146, 160, 174,
188, 202, 216, 230, 244, 258, and 272; altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein.
Accordingly, in another embodiment, the invention provides a method for preparing a Factor P-binding antibody consisting of a heavy chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 281 , 287, 293, 299, 305, 31 1 , 317, 323, 329, 335, 341 , 347, 353, 359, 365, 371 ,
377, 383, 389, and 395, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372,
378, 384, 390, and 396, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 283, 289, 295, 301 , 307, 313, 319, 325, 331 , 337, 343, 349, 355, 361 , 367, 373, 379, 385, 391 , and 397; and a light chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, and 398, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 285, 291 , 297, 303, 309, 315, 321 , 327, 333, 339, 345, 351 , 357, 363, 369, 375, 381 , 387, 393, and 399, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, and 400; altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein.
Accordingly, in another embodiment, the invention provides a method for preparing a Factor P-binding antibody optimized for expression in a mammalian cell consisting of: a full length heavy chain antibody sequence having a sequence selected from the group of SEQ ID NOs: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 and 275; and a full length light chain antibody sequence having a sequence selected from the group of SEQ ID NOs: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, and 276; altering at least one amino acid residue within the full length heavy chain antibody sequence and/or the full length light chain antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein. In one embodiment, the alteration of the heavy or light chain is in the framework region of the heavy or light chain.
The altered antibody sequence can also be prepared by screening antibody libraries having fixed CDR3 sequences or minimal essential binding determinants as described in US20050255552 and diversity on CDR1 and CDR2 sequences. The screening can be performed according to any screening technology appropriate for screening antibodies from antibody libraries, such as phage display technology.
Standard molecular biology techniques can be used to prepare and express the altered antibody sequence. The antibody encoded by the altered antibody sequence(s) is one that retains one, some or all of the functional properties of the Factor P-binding antibodies described herein, which functional properties include, but are not limited to, specifically binding to human and/or cynomolgus Factor P; and the antibody inhibit red blood cell lysis in a hemolytic assay.
In certain embodiments of the methods of engineering antibodies of the invention, mutations can be introduced randomly or selectively along all or part of an Factor P- binding antibody coding sequence and the resulting modified Factor P-binding antibodies can be screened for binding activity and/or other functional properties as described herein. Mutational methods have been described in the art. For example, PCT
Publication WO 02/092780 by Short describes methods for creating and screening antibody mutations using saturation mutagenesis, synthetic ligation assembly, or a combination thereof. Alternatively, PCT Publication WO 03/074679 by Lazar et al.
describes methods of using computational screening methods to optimize
physiochemical properties of antibodies. In certain embodiments of the invention antibodies have been engineered to remove sites of deamidation. Deamidation is known to cause structural and functional changes in a peptide or protein. Deamindation can result in decreased bioactivity, as well as alterations in pharmacokinetics and antigenicity of the protein pharmaceutical. {Anal Chem. 2005 Mar 1 ;77(5): 1432-9).
The functional properties of the altered antibodies can be assessed using standard assays available in the art and/or described herein, such as those set forth in the Examples (e.g., ELISAs).
Prophylactic and Therapeutic Uses
Antibodies that binds Factor P as described herein, can be used at a
therapeutically useful concentration for the treatment of a disease or disorder associated with increased complement activity by administering to a subject in need thereof an effective amount of the antibodies or antigen binding fragments of the invention. In a specific embodiment, the present invention provides a method of treating age-related macular degeneration (AMD) by administering to a subject in need thereof an effective amount of the antibodies of the invention.
The antibodies of the invention can be used, inter alia, to prevent progression of dry AMD to wet AMD, to slow and/or prevent progression of geographic atrophy, to treat or prevent macular edema, to reduce the frequency of Lucentis injection and to improve vision lost due to dry and wet AMD progression. It can also be used in combination with anti-VEGF therapies for the treatment of wet AMD patients.
Treatment and/or prevention of occular disease such as AMD can be determined by an ophthalmologist or health care professional using clinically relevant measurements of visual function and/or retinal anatomy. Treatment of AMD means any action (e.g., administration of an anti-Factor P antibody described herein) contemplated to improve or preserve visual function and/or retinal anatomy. In addition, prevention as it relates to AMD means any action (e.g., administration of an anti-Factor P antibody described herein) that prevents or slows a worsening in visual function, retinal anatomy, and/or an AMD disease parameter, as defined herein, in a patient at risk for said worsening.
Visual function may include, for example, visual acuity, visual acuity with low illumination, visual field, central visual field, peripheral vision, contrast sensitivity, dark adaptation, photostress recovery, color discrimination, reading speed, dependence on assistive devices (e.g., large typeface, magnifying devices, telescopes), facial recognition, proficiency at operating a motor vehicle, ability to perform one or more activities of daily living, and/or patient-reported satisfaction related to visual function. THus, treatment of AMD can be said to occur where a subject has an at least 10% decrease or lack of a 10% or more increase in time to a pre-specified degree of dark adaptation. In addition, treatment of AMD can be said to occur where a subject exhibits at least a 10% reduction or lack of a 10% or more increase in total area of central visual scotoma expressed as a visual angle determined by a qualified health care professional (i.e., opthalmologist).
Exemplary measures of visual function include Snellen visual acuity, ETDRS visual acuity, low-luminance visual acuity, Amsler grid, Goldmann visual field, Humphrey visual field, microperimetry, Pelli-Robson charts, SKILL card, Ishihara color plates, Farnsworth D15 or D100 color test, and validated tests for reading speed, facial recognition, driving simulations, and patient reported satisfaction. Thus, treatment of AMD can be said to be achieved upon a gain of or failure to lose 2 or more lines (or 10 letters) of vision on an ETDRS scale. In addition, treatment of AMD can be said to occur where a subject exhibits at least a 10% an increase or lack of 10% decrease in reading speed (words per minute). In addition, treamtne of AMD can be said to occur where a subject exhibits at least a 20% increase or lack of a 20% decrease in the proportion of correctly identified plates on an Ishihara test or sequenced disks ona Farnsworth test.
Undesirable aspects of retinal anatomy that may be treated or prevented include, for example, drusen, soft drusen, hard drusen, cuticular drusen, basal laminar drusen, confluent drusen, large drusen (e.g., greater than 125 microns in diameter), RPE atrophy, photoreceptor atrophy, geographic atrophy, choroidal neovascularization, subretinal neovascularization, retinal neovascularization, classic choroidal
neovascularization, occult choroidal neovascularization, retinal angiomatous proliferation, chorioretinal anastomosis, an abnormality of choroidal anatomy, subretinal hemorrhage, intraretinal hemorrhage, vitreous hemorrhage, macular scar, subretinal fibrosis, and retinal fibrosis. Thus, treatment of, for example, geographic atrophy can be determined by a 20% or more reduction in lesion growth rate as compared to control or previously documented growth rate in the same subject in the same eye.
Exemplary means of assessing retinal anatomy include funduscopy, fundus photography, fluorescein angiography, indocyanine green angiography, ocular coherence tomography (OCT), spectral domain ocular coherence tomography, scanning laser ophthalmoscopy, confocal microscopy, adaptive optics, fundus autofluorescence, biopsy, necropsy, and immunohistochemistry. Thus, AMD can be said to be treated in a subject upon a 10% reduction in the measurement of macular thickness as determined by OCT, and/or a reduction of hyperfluorescence as determined by fluorescein angiography.
Exemplary measures of retinal anatomy include drusen area, drusen volume, geographic atrophy lesion area, geographic atrophy growth rate, and neovascular membrane area.
In some embodiments, the present invention provides methods of treating a complement related disease or disorder by administering to a subject in need thereof an effective amount of the antibodies of the invention. Examples of known complement related diseases or disorders include: neurological disorders, multiple sclerosis, stroke, Guillain Barre Syndrome, traumatic brain injury, Parkinson's disease, disorders of inappropriate or undesirable complement activation, hemodialysis complications, hyperacute allograft rejection, xenograft rejection, interleukin-2 induced toxicity during IL- 2 therapy, inflammatory disorders, inflammation of autoimmune diseases, Crohn's disease, adult respiratory distress syndrome, thermal injury including burns or frostbite, post-ischemic reperfusion conditions, myocardial infarction, balloon angioplasty, post- pump syndrome in cardiopulmonary bypass or renal bypass, hemodialysis, renal ischemia, mesenteric artery reperfusion after acrotic reconstruction, infectious disease or sepsis, immune complex disorders and autoimmune diseases, rheumatoid arthritis, systemic lupus erythematosus (SLE), SLE nephritis, proliferative nephritis, hemolytic anemia, and myasthenia gravis. In addition, other known complement related disease are lung disease and disorders such as dyspnea, hemoptysis, ARDS, asthma, chronic obstructive pulmonary disease (COPD), emphysema, pulmonary embolisms and infarcts, pneumonia, fibrogenic dust diseases, inert dusts and minerals (e.g., silicon, coal dust, beryllium, and asbestos), pulmonary fibrosis, organic dust diseases, chemical injury (due to irritant gasses and chemicals, e.g., chlorine, phosgene, sulfur dioxide, hydrogen sulfide, nitrogen dioxide, ammonia, and hydrochloric acid), smoke injury, thermal injury (e.g., burn, freeze), asthma, allergy, bronchoconstriction, hypersensitivity pneumonitis, parasitic diseases, Goodpasture's Syndrome, pulmonary vasculitis, and immune complex-associated inflammation.
In a specific embodiment, the present invention provides methods of treating a complement related disease or disorder by administering to a subject in need thereof an effective amount of the antibodies of the invention, wherein said disease or disorder is asthma, arthritis (e.g., rheumatoid arthritis), autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, ischemia-reperfusion injuries, Barraquer-Simons Syndrome, hemodialysis, systemic lupus, lupus erythematosus, psoriasis, multiple sclerosis, transplantation, diseases of the central nervous system such as Alzheimer's disease and other neurodegenerative conditions, aHUS, glomerulonephritis, bullous pemphigoid or MPGN II.
In a specific embodiment, the present invention provides methods of treating glomerulonephritis by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention. Symptoms of
glomerulonephritis include, but not limited to, proteinuria; reduced glomerular filtration rate (GFR); serum electrolyte changes including azotemia (uremia, excessive blood urea nitrogen-BUN) and salt retention, leading to water retention resulting in hypertension and edema; hematuria and abnormal urinary sediments including red cell casts;
hypoalbuminemia; hyperlipidemia; and lipiduria. In a specific embodiment, the present invention provides methods of treating paroxysmal nocturnal hemoglobinuria (PNH) by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention.
In a specific embodiment, the present invention provides methods of reducing the dysfunction of the immune and hemostatic systems associated with extracorporeal circulation by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention. The antibodies of the present invention can be used in any procedure which involves circulating the patient's blood from a blood vessel of the patient, through a conduit, and back to a blood vessel of the patient, the conduit having a luminal surface comprising a material capable of causing at least one of complement activation, platelet activation, leukocyte activation, or platelet-leukocyte adhesion. Such procedures include, but are not limited to, all forms of ECC, as well as procedures involving the introduction of an artificial or foreign organ, tissue, or vessel into the blood circuit of a patient.
Subjects to be treated with therapeutic agents of the present invention can also be administered other therapeutic agents with know methods of treating conditions associated with macular degeneration, such as antibiotic treatments as described in U.S. Pat. No. 6,218,368. In other treatments, immunosuppressive agents such as
cyclosporine, are agents capable of suppressing immune responses. These agents include cytotoxic drugs, corticosteriods, nonsteroidal anti-inflammatory drugs (NSAIDs), specific T-lymphocyte immunosuppressants, and antibodies or fragments thereof (see Physicians' Desk Reference, 53rd edition, Medical Economics Company Inc., Montvale, N.J. (1999). Immunosuppressive treatment is typically continued at intervals for a period of a week, a month, three months, six months or a year. In some patients, treatment is administered for up to the rest of a patient's life.
When the therapeutic agents of the present invention are administered together with another agent, the two can be administered sequentially in either order or simultaneously. In some aspects, an antibody of the present invention is administered to a subject who is also receiving therapy with a second agent (e.g., verteporfin). In other aspects, the binding molecule is administered in conjunction with surgical treatments.
Suitable agents for combination treatment with Factor P binding antibodies include agents known in the art that are able to modulate the activities of complement components (see, e.g., U.S. Pat. No. 5,808, 109). Other agents have been reported to diminish complement-mediated activity. Such agents include: amino acids (Takada, Y. et al. Immunology 1978, 34, 509); phosphonate esters (Becker, L. Biochem. Biophy. Acta 1967, 147, 289); polyanionic substances (Conrow, R. B. et al. J. Med. Chem. 1980, 23, 242); sulfonyl fluorides (Hansch, C; Yoshimoto, M. J. Med. Chem. 1974, 17, 1 160, and references cited therein); polynucleotides (DeClercq, P. F. et al. Biochem. Biophys. Res. Commun. 1975, 67, 255); pimaric acids (Glovsky, M. M. et al. J. Immunol. 1969, 102, 1 ); porphines (Lapidus, M. and Tomasco, J. Immunopharmacol. 1981 , 3, 137); several antiinflammatories (Burge, J. J. et al. J. Immunol. 1978, 120, 1625); phenols (Muller- Eberhard, H. J. 1978, in Molecular Basis of Biological Degradative Processes, Berlin, R. D. et al., eds. Academic Press, New York, p. 65); and benzamidines (Vogt, W. et al Immunology 1979, 36, 138). Some of these agents function by general inhibition of proteases and esterases. Others are not specific to any particular intermediate step in the complement pathway, but, rather, inhibit more than one step of complement activation. Examples of the latter compounds include the benzamidines, which block C1 , C4 and C3b utilization (see, e.g., Vogt et al. Immunol. 1979, 36, 138).
Additional agents known in the art that can inhibit activity of complement components include K-76, a fungal metabolite from Stachybotrys (Corey et al., J. Amer. Chem. Soc. 104: 5551 , 1982). Both K-76 and K-76 COOH have been shown to inhibit complement mainly at the C3b step (Hong et al., J. Immunol. 122: 2418, 1979; Miyazaki et al., Microbiol. Immunol. 24: 1091 , 1980), and to prevent the generation of a
chemotactic factor from normal human complement (Bumpers et al., Lab. Clinc. Med. 102: 421 , 1983). At high concentrations of K-76 or K-76 COOH, some inhibition of the reactions of C2, C3, C6, C7, and C9 with their respective preceding intermediaries is exhibited. K-76 or K-76 COOH has also been reported to inhibit the C3b inactivator system of complement (Hong et al., J. Immunol. 127: 104-108, 1981 ). Other suitable agents for practicing methods of the present invention include griseofulvin (Weinberg, in Principles of Medicinal Chemistry, 2d Ed., Foye, W. O., ed., Lea & Febiger, Philadelphia, Pa., p. 813, 1981 ), isopannarin (Djura et al., Aust. J. Chem.36: 1057, 1983), and metabolites of Siphonodictyon coralli-phagum (Sullivan et al., Tetrahedron 37: 979, 1981 ).
A combination therapy regimen may be additive, or it may produce synergistic results (e.g., reductions in complement pathway activity more than expected for the combined use of the two agents). In some embodiments, the present invention provide a combination therapy for preventing and/or treating AMD or another complement related disease as described above with a Factor P binding antibody of the invention and an anti-angiogenic, such as anti-VEGF agent, or another anti-complement antibody such as an antibody or antigen binding fragment thereof that binds to complement factor 5 (C5).
Combination of anti-complement antibodies
In one aspect, the invention provides combinations of any one or more of the anti- Factor P with an additional antibody that binds to and inhibits the activity of a different component of the complement pathway. In particular, the invention includes any one or more of the anti-Factor P antibodies or antigen binding fragments described herein in combination with an antibody or antigen binding fragment that binds complement component 5 (C5). Examples of antibodies or antigen binding fragments thereof that bind to C5 and inhibit complement activation can be found, for example in U.S. Patent 8,241 ,628 (incorporated herein by reference). More specifically, antibodies or antigen binding fragments thereof that bind to C5 and inhibit the complement pathway are shown and described in Table 2. In one aspect the invention includes a combination of an anti- Factor P antibody or antigen binding fragment thereof as shown and described in Table 1 with the anti-C5 antibody 8109 from Table 2. More specifically, one aspect of the invention relates to a combination of antibody NVS962 from Table 1 (or an antigen binding fragment thereof) with antibody 8109 from Table 2 (or an antigen binding fragment thereof).
In one aspect the combinations of anti-Factor P and anti-C5 antibodies described herein demonstrate a syntergistic inhibition of the complement pathway, particularly the alternative complement pathway. Such inhibition can be demonstrated, for example, using the hemolytic or poly-IC assays described in the Examples below. Synergy in the inhibition of the alternative complement pathway, achieved using a combination of the anti-Factor-P and anti-C5 antibodies described herein can be determined using methods that are well known in the art. For example, a synergistic effect of the combination of anti-Factor P antibody and anti-C5 antibody can be determine relative to a merely additive effect using specific software, such as a Chalice Analyzer.
Briefly, Chalice Analyzer (Lehar et al, Nature Biotechnology 2009, 7:659) software can be used to determine whether the combination of complement inhibiting antibodies (e.g., anti-Factor P and anti-C5) acted synergistically to block complement activation. Combination effects can be characterized by comparing each data point's inhibition to that of a combination reference model that was derived from the single agent curves (Greco, Bravo, Parsons (1995). The search for synergy: a critical review from a response surface perspective. Pharmacol Rev 47(2): 331-85). In the Loewe additivity model (Loewe (1928). Die quantitativen Probleme der Pharmakologie. Erqebn. Physiol. 27: 47-187), /Loewe(Cx,CY) is the inhibition that satisfies (Cx//Cx) + (CY//CY) = 1 , and /CX Y are the effective concentrations at /Loewe for the fitted single agent curves. Loewe additivity is the generally accepted reference for synergy (Greco et al.), as it represents the combination response generated if X and Y are the same compound.
Potency shifting is usually shown using an isobologram (Greco et al.) which shows how much less drug is required in combination to achieve a desired effect level, when compared to the single agent doses needed to reach that effect. The choice of effect level for the isobologram display and combination index calculations can either be manually or automatically selected in the Chalice Analyzer. The automatic iso-level selection algorithm finds the observed /data with the the largest /data-/Loewe, excluding those points with /data exceeding the lesser single agent's /max. This exclusion is applied to ensure that the isobologram reflects the best synergy at levels covered by both single agents. Having selected an isobologram level /cut, the isobologram is drawn by identifying the locus of concentrations that correspond to crossing the chosen iso- level. The isobologram shows the standard isobolographic analysis of synergy compared to the Loewe dose-additive "drug-with-itself standard. For a specified isobologram level, the observed iso-effect contour (e.g., curved line in Figure 3) is displayed with the theoretical dose-additive contour (e.g., straight line in Figure 3), on an I Ceffect-normalized linear concentration scale for both substances in the combination. The Dose-additive reference is always a line connecting the two ICeffect concentrations. The I Ceffect crossing points are found by interpolating the fitted sigmoidal dose response curves. Potency shifting is scored as the combination index (Chou, Talalay (1984).
Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Requl 22: 27-55) CI. For a chosen iso-effect level /cut, CI I = (Cx/£Cx)i + (CY/£CY)i, where (Cx/£Cx)i for a particular data point is the ratio of the X compound's measured concentration to its effective concentration at the chosen inhibition level. The CI can be thought of as a rough estimate of how much drug was needed in combination relative to the single agent doses required to achieve the chosen effect level, and a value of 0.1 means that only a tenth of equivalent amounts of the single agents were needed for the combination to reach the same effect level. CI values in the range of 0.5-0.7 are typical for in vitro measurements of current clinical combinations (Greco et al.). A CI value of 1.0 is indicative of an additive effect of a combination of antibodies, while a CI value of less than 0.5 is indiciative of a strong synergistic effect resulting from the antibody combination. In the Chalice Analyzer, the best CI is reported from the many combination index values calculated for each /cut crossing concentration. Among all the measured CI values, the one with the largest signal-to-noise level is reported as the best combination index.
Combinations of anti-Factor P and anti-C5 antibodies as described herein can be administered singly or as a single composition. In addition, the relative dose of an anti- Factor P and anti-C5 antibody can be in a ratio of 1 :1 , or may be in a different ratio. The specific dose of an anti-Factor P antibody relative to an anti-C5 antibody may ultimately be determined by a treating physician or health care professional to achieve
improvement in the pathological condition being treated. For example, when a combination as described herein is used to treat AMD, a physician or health care professional may taylor the relative doses of the anti-Factor P and anti-C5 antibodies so as to achive optimal therapeutic benefit as determined using the measurements and criteria described herein.
Pharmaceutical Compositions
The invention provides pharmaceutical compositions comprising the Factor P- binding antibodies (intact or binding fragments) formulated together with a
pharmaceutically acceptable carrier. The compositions can additionally contain one or more other therapeutic agents that are suitable for treating or preventing, for example, pathological angiogeneis or tumor growth. Pharmaceutically acceptable carriers enhance or stabilize the composition, or can be used to facilitate preparation of the composition. Pharmaceutically acceptable carriers include solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible.
A pharmaceutical composition of the present invention can be administered by a variety of methods known in the art. The route and/or mode of administration vary depending upon the desired results. It is preferred that administration be intravitreal, intravenous, intramuscular, intraperitoneal, or subcutaneous, or administered proximal to the site of the target. The pharmaceutically acceptable carrier should be suitable for intravitreal, intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active compound, i.e., antibody, bispecific and multispecific molecule, may be coated in a material to protect the compound from the action of acids and other natural conditions that may inactivate the compound.
The composition should be sterile and fluid. Proper fluidity can be maintained, for example, by use of coating such as lecithin, by maintenance of required particle size in the case of dispersion and by use of surfactants. In many cases, it is preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol or sorbitol, and sodium chloride in the composition. Long-term absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate or gelatin.
Pharmaceutical compositions of the invention can be prepared in accordance with methods well known and routinely practiced in the art. See, e.g., Remington: The Science and Practice of Pharmacy, Mack Publishing Co., 20th ed., 2000; and Sustained and Controlled Release Drug Delivery Systems, J.R. Robinson, ed., Marcel Dekker, Inc., New York, 1978. Pharmaceutical compositions are preferably manufactured under GMP conditions. Typically, a therapeutically effective dose or efficacious dose of the Factor P- binding antibody is employed in the pharmaceutical compositions of the invention. The Factor P-binding antibodies are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those of skill in the art. Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, several divided doses may be
administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subjects to be treated; each unit contains a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
Actual dosage levels of the active ingredients in the pharmaceutical compositions of the present invention can be varied so as to obtain an amount of the active ingredient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient. The selected dosage level depends upon a variety of pharmacokinetic factors including the activity of the particular compositions of the present invention employed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors.
A physician or veterinarian can start doses of the antibodies of the invention employed in the pharmaceutical composition at levels lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved. In general, effective doses of the compositions of the present invention, for the treatment of an allergic inflammatory disorder described herein vary depending upon many different factors, including means of administration, target site, physiological state of the patient, whether the patient is human or an animal, other medications
administered, and whether treatment is prophylactic or therapeutic. Treatment dosages need to be titrated to optimize safety and efficacy. For systemic administration with an antibody, the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to 15 mg/kg, of the host body weight. For intravitreal administration with an antibody, the dosage may range from 0.1 mg/eye to 5mg/eye. For example, 0.1 mg/ml, 0.2 mg/ml, 0.3 mg/ml, 0.4 mg/ml, 0.5 mg/ml, 0.6 mg/ml, 0.7 mg/ml, 0.8 mg/ml, 0.9 mg/ml, 1 .0 mg/ml, 1 .1 mg/ml, 1 .2 mg/ml, 1 .3 mg/ml, 1.4 mg/ml, 1 .5 mg/ml, 1 .6 mg/ml, 1.7 mg/ml, 1 .8 mg/ml, 1 .9 mg/ml, 2.0 mg/ml, 2.1 mg/ml, 2.2 mg/ml, 2.3 mg/ml, 2.4 mg/ml, 2.5 mg/ml, 2.6 mg/ml, 2.7 mg/ml, 2.8 mg/ml, 2.9 mg/ml, 3.0 mg/ml, 3.1 mg/ml, 3.2 mg/ml, 3.3 mg/ml, 3.4 mg/ml, 3.5 mg/ml, 3.6 mg/ml, 3.7 mg/ml, 3.8 mg/ml, 3.9 mg/ml, 4.0 mg/ml, 4.1 mg/ml, 4.2 mg/ml, 4.3 mg/ml, 4.4 mg/ml, 4.5 mg/ml, 4.6 mg/ml, 4.7 mg/ml, 4.8 mg/ml, 4.9 mg/ml, or 5.0 mg/ml. An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months. An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months Antibody is usually administered on multiple occasions. Intervals between single dosages can be weekly, monthly or yearly. Intervals can also be irregular as indicated by measuring blood levels of Factor P-binding antibody in the patient. In addition alternative dosing intervals can be determined by a physician and administered monthly or as necessary to be efficacious. Efficacy is based on lesion growth, rate of Lucentis rescue, retinal thinckness as determined by Spectral Domain-optical Optical Coherence Tomography (SD-OCT), and secondary visual acuity. In some methods of systemic administration, dosage is adjusted to achieve a plasma antibody concentration of 1-1000 μg/ml and in some methods 25-500 μg/ml. Alternatively, antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the antibody in the patient. In general, humanized antibodies show longer half life than that of chimeric antibodies and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients continue to receive treatment for the rest of their lives. In therapeutic applications, a relatively high dosage at relatively short intervals is sometimes required until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete amelioration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime.
EXAMPLES
The following examples are provided to further illustrate the invention but not to limit its scope. Other variants of the invention will be readily apparent to one of ordinary skill in the art and are encompassed by the appended claims.
Example 1 : Generation of Affinity Matured Factor P Antibodies
A fully human phage display library was used to generate the Factor P binding antibodies described herein.
Biotinylated and non-biotinylated human and cynomolgus Factor P were used in solution and solid phase pannings. Standard panning were performed as well as RapMAT approaches (Prassler et al., (2009) Immunotherapy 1 (4):571 -583). Following affinity maturation (Knappik et al., (2000) J.Mol.Biol., 296:57-86) a set of 10 antibodies were subsequently chosen for conversion to a disulfide-bridged Fab format. The resulting disulfide bridged Fabs are shown in Table 1 (NVS962, NVS963, NVS964, NVS965, NVS966, NVS967).
Example 2: Further Antibody Optimization
The following example describes methods that may be used to further optimize antibodies described herein.
Removal of Deamidation Sites
Deamindation sites were identified by peptide mapping and size exclusion chromatography (SEC), run under reducing conditions. The deamidated material has decreased potency in a MAC deposition assay and decreased affinity for human and cyno FP as measured by Biacore and SET. The extent of deamidation increased over time (3 weeks), at higher temperatures (5 days at 37C), and under reducing conditions. Deamidation can be detected using an ion-exchange column resultin multiple peaks and observation of the additional, deamidated peak. Amino acid sequences that are most prone to deamidation are: SNG, LNG, LNN, ELN (Daugherty, A. and Mrsny, R. (2010) Current Trends in Monoclonal Antibody Development and Manufacturing. Springer. p103-129.). Accordingly, we engaged in a series of studies to remove the deamidation sites and test the modified antibodies for retained function.
Two Fabs, NVS962 and NVS965, were re-engineered to replace a deamindation site on the heavy chain, specifically occuring at an asparagine at position 30. The following new Fabs were generated to remove the deamindation site and corresponding amino acid replacements shown in Table 2.
Table 2: Deamidated Fabs
Deaminadated Fab N30 replaced with: Modified Fab
NVS962 Serine NVS962-S
Glutamine NVS962-Q
Glycine NVS962-G
Threonine NVS962-T
NVS965 Threonine NVS965-T
Glutamine NVS965-Q
Serine NVS965-S An additional Fab that was generated replaced serine 31 with an alanine in Fab NVS962, generating Fab NVS962-S31A. The sequences of the modified Fabs is shown in Table 1 .
Removal of Cleavage Sites
Further optimization was conducted on NVS962-S and NVS965-S to remove a cleavage site in the heavy chain CDR3. Specifically the heavy chain was cleaved at Y102S103. The following table describes the amino acid substitutions that were made to destroy the cleavage site. The sequences of the modified Fabs is shown in Table 1 .
Table 3: Modified Fabs
Figure imgf000131_0001
Example 3: Characterization of Optimized Antibodies
The following example describes methods that may be used to measure antibody affinity. These and other methods of measuring binding affinity are known in the art.
Affinity Determination
Antibody affinity for Factor P was measured by surface plasmon resonance (SPR) using a Biacore T200 (Biacore) and solution equilibrium titration (SET).
Explanations of each technology and corresponding mean results for Factor P binding are described below. Modelling assumptions take into account concentrations of Factor P in the system, kinetics of Factor P biosynthesis and half-life, as well as the desired dosing schedule, and suggest that a Fab with an affinity of greater than 500 pM for Factor P is sufficient to lower levels of free Factor P.
Biacore Determination
The kinetics of an interaction, i.e. the rates of complex formation (ka) and dissociation (kd), can be determined from the information in a sensorgram. If binding occurs as sample passes over a prepared sensor surface, the response in the sensorgram increases. If equilibrium is reached a constant signal will be seen. Replacing sample with buffer causes the bound molecules to dissociate and the response decreases. Biacore evaluation software generates the values of ka and kd by fitting the data to interaction models (Table 4).
Biacore kinetic experiments were done with the BIAcore T100 (GE Healthcare) using CM5 sensor chips (GE Healthcare, BR-1005-30) at 25°C. The running buffer was HBS-EP(+) (GE Healthcare, BR-1001 -88). Briefly, the following steps were carried out to determine binding affinity.
• Prepare anti-FP IqG immobilized sensor chip: Mouse anti-FP monnoclonal
antibody (Quidel, A235) (30ug/ml in acetate pH5.0 coupling buffer (GE
Healthcare, BR-1003-51 )) was coupled to two different flow cells (Fc1 and 2) on a CM5 chip at 10ul/min flow rate for 600 seconds by using amino-coupling procedure according to the supplier's instruction (GE Healthcare, BR-1000-50). The final immobilized level will be >7000RU.
• Capture FP on second flow cell: 1 ug/ml of FP in running buffer was injected at 10ul/min on second flow cell (Fc2) to reach capture level -20 RU for Fab or ~7 RU for IgG kinetics analysis.
• Inject anti-FP Fab or IqG at different concentration on both flow cells: Inject anti- FP solution (0.3125nM~10nM in running buffer; at 1 :2 serial dilutions) on both flow cells (Fc1 and 2) at 60ul/min for 240 seconds.
• Dissociation: Inject HBS-EP(+) running buffer at 60ul/min on both flow cells to monitor the dissociation between FP and anti-FP Fab/lgG. Dissociation time was set at 2400 seconds for 5nM and 2.5M Fab/lgG concentrations and at 300 seconds for all other concentrations including another 5nM Fab/lgG
concentration.
• Regeneration: Regeneration was performed at the end of each cycle on both flow cells with 10 mM Glycine-HCI pH1.7 (provided by GE Healthcare)+ 0.05% P20 surfactant (GE Healthcare, BR-1000-54) at a flow rate of 60ul/min for 15 seconds twice.
• Kinetics analysis: Kinetic rate constants was obtained by applying 1 :1 binding model with BIAevaluation 1 .1 software, wherein the Rmax values were fit locally.
The results of the Biacore binding kinetics determination are shown in Table 4. As shown the antibodies described herein exhibit high affinity binding to human Factor P, with KD values typically less than or equal to 1 nM, and in many cases less than or equal to 200 pM. These antibodies also show very high affinity to cyno Factor P (binding affinity less than 500 pM). SET Determination
In contrast to kinetic assays using sensor surfaces, such as SPR, SET is a method which determines affinities in solution. It is an equilibrium measurement that does not deliver kinetic data.
In SET, a constant amount of antibody is incubated with different concentrations of antigen until equilibrium is reached. The concentration of free antibody in the equilibrated solution is determined by applying the solution on an antigen coated MSD™ plate (Meso Scale Discovery™) followed by incubation with an ECL-labeled secondary antibody and measurement of signal intensity. At low antigen concentrations, a strong signal is achieved (high concentration of free antibody which binds to the antigen on the plate) whereas for high antigen concentration, the antibody is completely antigen- captured, resulting in a low signal. If a sufficient number of antigen concentrations in a matching range are available, the titration curve allows for a reasonable determination of the affinity, using the appropriate fit model. For a complete titration, antigen
concentrations of at least 10-fold higher than the anticipated KD have to be applied. The constant concentration of antibody applied in the assay should be in the range of, or below, the KD (Table 4).
For KD determination by solution equilibrium titration (SET), monomer fractions of antibody protein were used (at least 90% monomer content, analyzed by analytical SEC; Superdex75 (Amersham Pharmacia) for Fab, or Tosoh G3000SWXL (Tosoh Bioscience) for IgG, respectively).
Affinity determination in solution was basically performed as described in the literature (Friguet et al. 305-19). In order to improve the sensitivity and accuracy of the SET method, it was transferred from classical ELISA to ECL based technology (Haenel et al., 2005).
1 mg/ml goat-anti-human (Fab)2 fragment specific antibodies (Dianova) were labeled with MSD Sulfo-TAG™ NHS-Ester (Meso Scale Discovery, Gaithersburg, MD, USA) according to the manufacturer's instructions.
Human Factor P (Complement Technology cat#: A139) and Cyno Factor P purified from cyno serum (protocol adapted from Nakano, et al., (1986) J Immunol Methods 90:77-83) were coated on standard binding MSD plates (Meso-Scale
Discovery, 384-well: MSD cat#: L21XA, 96-well: MSD cat#: L15XA) at 0.2-0.3 g/ml in 25μΙ PBS and incubated overnight at 4°C. Factor P inhibitors were diluted to a fixed concentration (1 pM or 10pM) in incubation buffer (PBS with 2% BSA (Sigma cat#:
A4503) and 1 % Tween20 and 1 % Triton-X (Sigma cat#: 234729)), and added to a serial dilution of Factor P (human or cyno) in incubation buffer. Samples were allowed to reach equilibrium by incubation at RT overnight. Plates were washed 3x in wash buffer (PBS with 0.05% Tween20), and blocked with 10ΟμΙ incubation buffer at RT for 2hrs. Plates were washed 3x in wash buffer. Sample containing Factor P inhibitors and Factor P titration were added to the plate (25μΙ), and incubated at RT for 15min. Plates were washed 3x in wash buffer. 25μΙ detection antibody was added (Anti-Human (Goat) Sulfo- TAG, 1 :1000 in incubation buffer, MSD cat#: R32AJ-1 ), and incubated at RT for 60min. Plates were washed 3x in wash buffer, and 50μΙ of 1 x MSD Read buffer T was added (with surfactant, MSD cat#: R92TC-1 ). Plates were read on a MSD Spector Imager 6000. Data was analyzed using GraphPad Prism software v4, with background (an average of wells containing no Fab) subtracted from each value. X-axis values (concentration of Factor P in solution) were transformed into log 10x. KD values (KD) were fitted from the following model:
Fab:
Y=(Top-((Top/(2xFab))x((((10Ax)+Fab)+KD)-(((((10Ax)+Fab)+KD)x(((10Ax)+Fab)+KD)) - ((4x(10Ax))xFab))A0.5))))
Top= signal at antigen concentration = 0
x= concentration of Factor P in solution
Fab= concentration of applied monovalent analyte (Fab)
Table 4: Affinity Binding of Factor P Antibodies
Figure imgf000134_0001
NVS967 Human 40 60 1 x 106 9 x 10"5
Cyno 280 315 1 x 106 6 x 10"4
NVS962-Q Human 1061 496 6.27 x 105 3.1 1 x 10"4
Cyno 930 475 5.55 x 105 2.64x 10"4
NVS962-S Human 156 161 7.3 x 10b 1.17 x 10"4
Cyno 141 127 5.24 x 105 6.66 x 10"5
NVS962-T Human 251 131 6.39 x 105 8.35 x 10"5
Cyno 354 175 4.88 x 105 8.56 x 10"5
NVS962-G Human 953 1 140 3.87 x 10b 4.4 x 10"4
Cyno 567 1080 3.13 x 10b 3.39 x 10"4
NVS962-S31A Human 189 225 5.04 x 105 1.13 x 10"4
Cyno 189 234 3.72 x 105 8.7 x10"5
NVS965-Q Human 301 138 2.65 x 106 3.66 x 10"4
Cyno 201 215 2.77 x 106 5.94 x 10"4
NVS965-S Human 51 27 3.69 x 10 9.9 x 10"b
Cyno 36 60 2.71 x 106 1.63 x 10"4
NVS965-T Human 52 51 .4 2.37 x 106 1.03 x 10"4
Cyno 44 74.6 2.17 x 10s 1.51 x 10"4
NVS808 Human 107 195 7.91 x 10b 1.54 x 10"4
Cyno ND ND ND ND
NVS806 Human 18 52 4.34 x 10b 2.24 x10"b
Cyno ND ND ND ND
NVS807 Human 27 106 1.21 x 106 1.23 x 10"4
Cyno ND ND ND ND
NVS804 Human 0.8 5 2.72 x 10 1.29 x 10"b
Cyno ND ND ND ND
NVS805 Human 0.3 3 3.61 x 106 9.88 x 10-6
Cyno ND ND ND ND
NVS809 Human 4.2 24.7 4.25 x 106 1.04 x 10"4
Cyno ND ND ND ND Example 4: Factor P Antibodies Inhibit the Alternative Complement Pathway Hemolysis Assay
In hemolytic techniques, all of the complement components must be present and functional. Therefore hemolytic techniques can screen both functional integrity and deficiencies of the complement system (van et al., 1980;Minh et al., 1983;Tanaka et al., 1986). To measure the functional capacity of the classical pathway, sheep red blood cells coated with hemolysin (rabbit IgG to sheep red blood cells) or chicken red blood cells that are sensitized with rabbit anti-chicken antibodies are used as target cells (sensitized cells). These Ag-Ab complexes activate the classical pathway and result in lysis of the target cells when the components are functional and present in adequate concentration. To determine the functional capacity of the alternative pathway in human and cynomolgus sera, rabbit red blood cells are used as the target cell (see U.S. Pat. No. 6,087, 120).
The hemolytic assay is a basic functional assay that tests for complement activation and has been used to evaluate the ability of anti-human FP mAbs and Fab molecules to block lysis of red blood cells (RBCs) by complement pathways. In vitro and in vivo inhibition of complement activity by a single-chain Fv fragment recognizing human C5 can be measured using a haemolytic assay (Thomas et al., 1996;Rinder et al., 1995;Rinder et al., 1995) . Blockade of C5a and C5b-9 generation inhibits leukocyte and platelet activation during extracorporeal circulation. Briefly, for classical pathway assays, sensitized red blood cells (e.g., chicken RBCs) are used as targets for lysis by
complement proteins present in serum. The following assay is of interest for the characterization and screening of Factor P antibodies for their inhibition of the alternative complement pathway.
This procedure was adapted from (Rinder et al., 1995;Thomas et al., 1996).
Reagents:
• Rabbit red blood cells (Rb RBCs) - Lampire, Cat# 7246408
• Human serum - Novartis Blood Research Program; or Cyno serum - Alpha
Genesis
• Gelatin veronal buffer (GVB) - Boston BioProducts, Cat# IBB-300
• EGTA - Boston BioProducts, Cat# BM-151
• MgCI2
• U-bottom 96-well plate - Corning, Cat# 3795
• Flat-bottom 96-well plate - Corning, Cat# 3370
• NP-40 - Sigma, Cat# 74385 Protocol:
Rabbit red blood cells (RBCs) were washed and adjusted to 8.33x107 cells/ml in GVB/EGTA/Mg++. 50μΙ Fab diluted in GVB was added to wells in a 96-well round bottom plate. 50μΙ serum diluted in GVB with EGTA and Mg++ was then added. Control wells were prepared in the following manner: serum wihtout Fab (negative control) and cells plus 0.1 % NP-40 (100% lysis control), and NP-40 blank wells. Serum with and without Fab and controls were incubated at room temperature for 30 minutes. At that point, 30μΙ Rb RBCs were added to sample and control wells and 30μΙ of buffer was added to the blank wells. The cells were generally incubated for 30 minutes at 37°C and the plate centrifuged at 2000rpm for 5min. The supernatant was harvested and transferred to a flat-bottom plate. The absorbance of the supernatant was read at OD415 and OD570. Percent hemolysis was calculated using the formula below.
(ODsample - ODserum .blank ) - (OD 0%lysis - ODbuffer .blank ) (OD100 %lysis - ODNP 40 blank ) - (OD 0%fy 'sis - ODbuffer .blank )
Table 5 exemplifies of the ability of the Factor P antibodies and antigen binding fragments to inhibit hemolysis in 10% human or 20% cynomolgus serum. Each of the Factor P antibodies described herein inhibited hemolysis with an IC50 of less than or equal to 50 nM.
In contrast, when the assay was performed using sensitized red blood cells in order to examine activation of the classical complement pathway, the Factor P antibodies described herein were found not to inhibit the classical complement pathway (data not shown).
C3b Deposition Assay
One method of measuring the inhibitor activity against the complement C3 in the alternative pathway is to measure its breakdown product, C3b, depositing on zymosan. This ELISA based assay was performed according to the following steps: 25μΙ of 1 mg/ml Zymosan A (Sigma Z4250) in carbonate buffer, pH 9.6 (Pierce Cat# 28382) was coated on Maxisorp 384-well ELISA plate (Nunc 464718) overnight at 4°C. On the following day, the zymosan-coated plate was aspirated and blocked with 100μΙ per well of ELISA blocking buffer, Synblock (AbD Serotec BUF034C) for 2h at room temperature. In a separate reaction, the inhibitors, serially diluted in gelatin veronal buffer (Boston
Bioproducts IBB320-10mM Barbital, 145mM NaCI, 0.1 % Gelatin, 0.5mM MgCI2, 10mM EGTA) were added to 10% serum supplemented with MgCI2 and EGTA for a final total reaction concentration of 1 mM MgCI2 and 10mM EGTA. The positive control contained no inhibitor and negative control had 25mM EDTA. The mixture was allowed to reach equilibrium by incubating at room temperature for 30min. To remove the blocking buffer, the buffer was aspirated and the plate was washed once with TBS/0.05% Tween-20. 25μΙ per well of the 10% serum containing the inhibitors or controls was added to the plate and incubated at 37°C for 30min (previously determined by time-course to be within the linear range of C3b deposition on zymosan.) After the 30min incubation, the plate was washed three times with TBS/0.05% Tween-20. To detect C3b deposition on zymosan, 25μΙ per well of chicken anti-human C3-HRP conjugated polyclonal antibody (Immunology Consultants Laboratory, Inc. Cat# CC3-80P-1 ) diluted according to manufacturer in PBS with 2%BSA Fraction V (Fisher Cat# ICN 16006980), 0.1 %
Tween20 (Sigma Cat# P1379), and 0.1 % TritonX-100 (Sigma Cat# P234729) was added to the plate and incubate at room temperature for 1 h. Afterward, the plate was washed three times with TBS/0.05% Tween-20 and then add 25μΙ of Ultra TMB Substrate Solution (Pierce Cat#34028.) When the solution in the well turned blue, the reaction was stoppedwith 15μΙ of 2N sulfuric acid. The plate was read at 450nm using the
Spectromax with correction for the plastic plate at 570nm (OD 45o-570nm reading.) The percentage of C3b deposition on zymosan was calculated using the following formula:
[(OD no inhibitor - OD 25mM EDTA)- (OD sample - OD 25mM EDTA)]
% C3b Deposition = 100 - 100 *
(OD no inhibitor " OD 25mM EDTA)
Each of the antibodies tested were shown to inhibit C3b deposition with an IC50 of at least less than or equal to 10 nM (Table 5).
MAC Deposition Assay
Another assay that was used to determine the ability of the Factor P antibodies to inhibit the alternative complement pathway was to measure the ability of the antibodies to inhibit the generation of the membrane attack complex (MAC), which is downstream of the C3 convertase and the activity of Factor P. Briefly, Zymosan A (Sigma ) was coated on a plate at 1 mg/ml in carbonate buffer, pH 9.5, to activate the Alternative Pathway. Fabs were pre-incubated with serum (20% serum, 5mM MgCI2, 10mM EDTA), then added to the plate and incubated overnight at room temperature. After washing the plate three times with TBST, MAC was detected by incubation with anti-C5b-9-ALP (Diatec) for 1 h, followed by three washes with TBST, and incubation with 4-methylumbelliferyl phosphate (Fisher) supplemented with 2mM MgCI2 for 30 minutes. The reaction was stopped with 0.2M EDTA, and the plate was read at ex=355nm , em=460nm. Inhibition of MAC deposition was calculated for each sample relative to baseline (EDTA treated human serum) and positive control (human serum), and used to generate the IC50 curve with PRISM.
Table 5 shows data demonstrating the ability of the Factor P antibodies to inhibit the deposition of MAC, thus indicating that the antibodies inhibited the alternative complement pathway. Specifically, the antibodies inhibited MAC deposition with an IC50 of less than or equal to 25nM.
C3a Deposition Assay
Another method used to assess the ability of Factor P antibodies to inhibit the alternative complement pathway is to measure the ability of antibodies to inhibit the generation of C3a following cleavage of C3 by C3 convertase. The assay was carried out on zymosan-coated Maxisorp plates coated at 10 mg/ml and 10% and 20% human serum pre-incubated with anti-properdin Fab diluted in a 2n series. The serum was added to the plates for 30 minutes at which time the serum was collected for assessment of C3a generation.
Maxisorp plates were coated with anti-C3a des-arg neo antibody (1 ug/ml) overnight, washed three times, and blocked with diluent for two hours at room
temperature. Following aspiration of the diluent, serum was added for one hour. Plates were washed three times and a 100 uL/well detection antibody Mouse anti-Human C3a- Biotin 1 :1000 diluted in diluent was added. Following an additional one hour incubation, a streptavidin-HRP secondary antibody diluted 1 :5000 in diluent was added to the wells for one hour at room temperature. Plates were washed four times before the addition of TMB detection substrate. The reaction was stopped using standard stop solution and absorbance was read at 450-570 nm.
In parallel to the addition of the serum, a standard curve was produced using purified C3a des-arg diluted in serum. Starting at 5ug/ml, C3a des-arg was serially diluted 1 :4 to generate a 7 point curve. The standard curve wells were treated, washed, and read as above. Table 5: Functional Analysis of Factor P Antibodies
Hemolytic Inhibition
Zymosan-
MAC assay of C3a C3b IC50 generation,
Factor P Antibody Factor P Species Deposition, IC50
20% Serum (nM), 20%
(nM),
EC50 (nM) 10% human
10%
serum serum serum (nM)
NVS962 Human 18.79 2.63 13.18 78.42
Cyno 12.08 9.91 16.14 ND
NVS965 Human 17.32 1.54 7.527 31.33
Cyno 22.17 6.36 13.20 ND
NVS963 Human ND 2.34 10.11 65.08
Cyno ND 9.75 14.53 ND
NVS966 Human ND 1.66 7.154 41.11
Cyno ND 6.62 13.00 ND
NVS964 Human ND 1.54 9.26 42.18
Cyno ND 6.59 13.41 ND
NVS967 Human ND 2.53 9.61 43.53
Cyno ND 5.92 14.01 ND
NVS962-Q Human 23.12 0.64 15.27 ND
Cyno 20.88 1.62 14.7 ND
NVS962-S Human 6.53 1.53 8.21 ND
Cyno 15.57 1.94 12.02 ND
NVS962-T Human 6.61 1.75 9.31 ND
Cyno 5.42 3.10 12.54 ND
NVS962-G Human 12.68 1.18 13.39 ND
Cyno 9.27 2.94 14.23 ND
NVS962-S31A Human 9.96 1.15 12.79 ND
Cyno 7.86 3.12 11.61 ND
NVS965-Q Human 15.40 1.64 9.71 ND
Cyno 9.39 3.30 14.71 ND
NVS965-S Human 7.32 1.39 7.89 ND
Cyno 8.12 1.57 12.02 ND
NVS965-T Human 13.80 0.77 7.15 ND Cyno 10.98 1.91 12.54 ND
NVS808 Human 6.51 ND 13.28 ND
Cyno 8.38 ND 15.53 ND
NVS806 Human 5.74 ND 10.14 ND
Cyno 6.51 ND 13.93 ND
NVS807 Human 5.40 ND 12.91 ND
Cyno 6.35 ND 12.57 ND
NVS804 Human 5.85 ND 12.38 ND
Cyno 8.51 ND 14.44 ND
NVS805 Human 5.90 ND 12.82 ND
Cyno 5.95 ND 22.11 ND
NVS809 Human 6.46 ND 12.1 ND
Cyno 5.90 ND 12.78 ND
ND: Not Determined
Example 5: Species Cross Reactivity
In order to determine whether, in addition to human and cynomolgus, the anti- Factor P antibodies described herein would bind to Factor P from other species, MAC deposition and hemolytic assays were carried out as described above. BIAcore analysis, or hemolytic assays were carried out as described above. The serum concentrations used for each species were as follows: 10 and 20% rabbit, 10 and 20% cynomolgus, and 10 and 20% human sera. Rat Factor P binding was assessed by BIAcore. As shown in Table 6 below, the Factor P antibodies were able to cross react with several species, including rabbit, rat and cynomolgus.
Table 6: Species cross-reactivity
Antibody Human Rat Rabbit Cyno
NVS962 X X X X
NVS962-S X X X X
NVS801 X X X X
NVS965 X ND ND X NVS965-S X ND ND X
NVS808 X ND X X
NVS806 X ND X X
ND: nol determined
Example 6: Epitope Mapping
Factor P is comprised of several Thrombospondin repeat domains (TSR 0-6). The TSRO domain is also referred to as the N terminal domain. Epitope mapping of the Factor P Fabs was performed by creating mouse and human chimeras for each TSR. Previous functional assays showed that the Fabs do not bind to mouse Factor P
(hemolytic assays), although each of the chimeras was functional in Factor P-depleted serum. Using this method it was determined that all of the Fabs bind to TSR 5 (SEQ ID NO: 406). Figure 1 C shows the antibodies bind region B of TSR5. The commercially- available antibody, A233, was shown not to bind in this region. Binding can be assessed by ELISA or Biacore using standard methods. For one Ab, NVS487, the data was not conclusive due to cross reactivity to mouse Factor P. Sequence alignment between mouse and human Factor P TSR5 domain shows the epitope includes the amino acids of SEQ ID NO: 408.
Example 7: In vivo inhibition of the Alternative Complement Pathway
Experiments were performed in cynomolgus money with antibodies of the invention to determine their ability to inhibit the alternative complement pathway.
The test item, NVS962, was administered at the dose levels shown in Table 7. The route of administration was either intravitral (IVT) or intravenous (IV).
Table 7: In Vivo Study Design
Group Group Dose Route Dose volume Animals/group number description level of (ML/injection)
Male Female dosing 1 Control 0 (vehicle) IVT 50/IVT 1 1
IV injection
100/IV
injection
2 Low IVT 1 mg/eye IVT 50/IVT 1 1
(2 injection
mg/monkey)
3 IV 10 IV 100/IV 1 1
mg/monkey injection
4 High IVT 5 mg/eye IVT 50/IVT 1 1
(10 injection
mg/monkey)
The test item and vehicle solutions (vehicle: 10 mM His/His-HCI; 10% trehalose; 0.02% Tween 20; pH 5.5) were administered intravitreally and intravenously on days 1 , 15, and 29 of the study as indicated in Table 7.
Assessment of toxicity was based on mortality, clinical observations, body weights, pharmacodynamics (hemolytic analysis), ophthalmic examinations, intraocular pressure measurements, electroretinography, hematology, clinical chemistry, organ weights, and pathology.
There were no mortalities during the study and no test item related findings were seen after evaluation of clinical signs, body weights, ophthalmic examinations, intraocular pressure measurements, electroretinography, hematology, clinical chemistry, organ weights, and pathology.
Complement mediated hemolytic activity was measured using the hemolytic assay described above (see Example 4). Analysis of the hemolytic assay data showed that IV administration of NVS962 led to a complete or nearly complete but short-lived, inhibition of hemolytic complement activity immediately after administration. When administered by the IVT route at a dose of 1 mg/eye, the test item had little or no effect on serum hemolytic complement activity. At 5 mg/eye and in 10% cynomolgus serum, a complete or nearly complete inhibition of hemolytic complement activity was observed. Example 8: Synergistic inhibition of the Alternative Complement Pathway by Antibody
Combinations
Hemolysis Assay
Hemolytic assays using the Fab versions of the anti-C5 antibody 8109 from Table 2 and anti-Factor P antibody NVS962 from Table 1 were performed as described in Example 4.
Figure 2 exemplifies of the ability of the Factor P antibodies and antigen binding fragments in combinations with anti-C5 antibodies and antigen binding fragments to inhibit hemolysis in 20% human serum. 500nM anti-factor P and 500nM anti-C5 Fabs individually demonstrate no inhibition of hemolysis when incubated for 60 mins. In contrast, the combination of anti-factor P and anti-C5 antibodies at the same
concentration and at concentrations as low as 167nM demonstrate near complete inhibition of hemolysis. In addition, the near complete inhibition of hemolysis lasts for up to 250 minutes.
Data from the hemolytic assay was used with Chalice Analyzer software to determine whether the combination of complement inhibiting antibodies acted synergistically to block complement activation. Combination effects can be characterized by comparing each data point's inhibition to that of a combination reference model that was derived from the single agent curves (Greco, Bravo, Parsons (1995). The search for synergy: a critical review from a response surface perspective. Pharmacol Rev 47(2): 331-85). In the Loewe additivity model (Loewe (1928). Die quantitativen Probleme der Pharmakologie. Erqebn. Physiol. 27: 47-187), /i_oewe(Cx,CY) is the inhibition that satisfies (Cx//Cx) + (CY//CY) = 1 , and /CX Y are the effective concentrations at /Loewe for the fitted single agent curves. Loewe additivity is the generally accepted reference for synergy (Greco et al.), as it represents the combination response generated if X and Y are the same compound.
Potency shifting is usually shown using an isobologram (Greco et al.) which shows how much less drug is required in combination to achieve a desired effect level, when compared to the single agent doses needed to reach that effect. The choice of effect level for the isobologram display and combination index calculations can either be manually or automatically selected in the Chalice Analyzer. The automatic iso-level selection algorithm finds the observed /data with the the largest
Figure imgf000144_0001
excluding those points with /data exceeding the lesser single agent's /max. This exclusion is applied to ensure that the isobologram reflects the best synergy at levels covered by both single agents. Having selected an isobologram level /cut, the isobologram is drawn by identifying the locus of concentrations that correspond to crossing the chosen iso- level. The isobologram shows the standard isobolographic analysis of synergy compared to the Loewe dose-additive "drug-with-itself standard. For a specified isobologram level, the observed iso-effect contour (e.g., curved line in Figure 3) is displayed with the theoretical dose-additive contour (e.g., straight line in Figure 3), on an I Ceffect-normalized linear concentration scale for both substances in the combination. The Dose-additive reference is always a line connecting the two ICeffect concentrations. The I Ceffect crossing points are found by interpolating the fitted sigmoidal dose response curves.
Potency shifting is scored as the combination index (Chou, Talalay (1984). Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Requl 22: 27-55) CI. For a chosen iso-effect level /cut, CI I = (Cx/£Cx)i + (CY/£CY)i, where (Cx/£Cx)i for a particular data point is the ratio of the X compound's measured concentration to its effective concentration at the chosen inhibition level. The CI can be thought of as a rough estimate of how much drug was needed in combination relative to the single agent doses required to achieve the chosen effect level, and a value of 0.1 means that only a tenth of equivalent amounts of the single agents were needed for the combination to reach the same effect level. CI values in the range of 0.5-0.7 are typical for in vitro measurements of current clinical combinations (Greco et al.). A CI value of 1.0 is indicative of an additive effect of a combination of antibodies, while a CI value of less than 0.5 is indiciative of a synergistic effect resulting from the antibody combination. In the Chalice Analyzer, the best CI is reported from the many combination index values calculated for each /cut crossing concentration. Among all the measured CI values, the one with the largest signal-to- noise level is reported as the best combination index.
Data from the hemolytic assay were expressed as % inhibition and loaded into an 8x8 Excel table, in which the antibodies concentrations were expressed as uM values. The Excel template was uploaded to the Chalice software (Lehar et al. 2009) and the combination index was generated by creating an isobologram curve using IC20 for each antibody (CI = CX/ICX + CY/ICY, where ICX and ICy are, respectively, the concentrations of anti-factor P antibody and anti-C5 antibody alone that result in a 20% inhibition effect and Cx and Cy are the concentrations of each drug in the mixture that yield 20 % inhibition). The combination index at 20% inhibition is 0.36, indicating synergy between anti-factor P antibody and anti-C5 antibody (Figure 3). Marcophage Infiltration
The effect of anti-fP and anti-C5 Fabs individually or in combination were assessed in vivo using the poly-IC murine model of ocular inflammation. Mice were injected i.v. with synthetic dsRNA analog, poly l:C in 0.1 ml PBS systemically into C57BL/6 mice along with anti-fP (antibody NVS962 from Table 1 ) and anti-C5 antibodies (antibody 8019 from Table 2) individually or in combination. Mice were euthanized at indicated time points. Eyes and retinas were collected and protein extracts were prepared for cytokine and chemokine analysis using a multiplex assay (Pierce). To determine retinal leukocyte infiltration, eyes were fixed in 4% paraformaldehyde and stained with Alexa Fluor-488 conjugated F4/80 antibody for macrophages. The retinas were flat mounted with the retinal vasculature orientated superiorly onto a glass slide and coversliped with a drop of Vectashield mounting medium (Vector Laboratories Inc, Burtingame, CA). Fluorescent images of five (500um) regions on each retina were captured using the Axiocam MR3 camera on a Axio.lmageMI microscope (Zeiss). The number of neutrophils and macrophages was quantified with Axiovision software (Version 4.5 Zeiss). Using optical coherence tomography (OCT), images of retinas were obtained and analysed from mice treated with poly l:C. These results (Figure 4) demonstrate that at the highest concentrations tested (20ug) no greater than 45% inhibition of macrophage inhibition was observed. In contrast, combinations of the anti- Factor P and C5 antibodies at concentrations as low as 2 ug demonstrated 79% inhibition and increasing the concentration achieved 100% inhibition (compared to only 13% and 32% inhibition respectively for the anti-C5 and anti-Factor P antibodies individually).
Data from in vivo poly-IC model (macrophage infiltration) described in the preceding paragraph were expressed as % inhibition and loaded into a 4x4 Excel table, in which the antibody doses were expressed as ug values. The Excel template was uploaded to the Chalice analyzer (described above) and the combination index was generated by creating an isobologram curve using IC50 for each antibody (CI = CX/ICX + CY/ICY, where ICX and ICy are, respectively, the concentrations of anti-factor P antibody and anti-C5 antibody alone that result in a 50% inhibition effect and Cx and Cy are the concentrations of each drug in the mixture that yield 50 % inhibition). The combination index at 50% inhibition is 0.42 (See Figure 5), indicating synergy between anti-factor P antibody and anti-C5 antibody.

Claims

Claims
1 . An antibody, or antigen binding fragment thereof, that binds a region of Factor
P comprising SEQ ID NO: 408.
2. An antibody, or antigen binding fragment thereof, that binds a region of Factor
P comprising SEQ ID NO: 407.
3. The antibody, or antigen binding fragment of any preceding claim that binds
Factor P with a KD of less than or equal to 1.2 nM as measured by solution equilibrium titration.
4. The antibody, or antigen binding fragment of any preceding claim that binds
Factor P with a KD of less than or equal to 500 pM.
5. The antibody, or antigen binding fragment of any preceding claim that binds
Factor P with a KD of less than or equal to 200 pM.
6. The antibody, or antigen binding fragment, of any preceding claim, wherein said antibody binds human Factor P.
7. The antibody, or antigen binding fragment, any preceding claim that also binds cynomologous monkey, rabbit or rat Factor P.
8. The isolated antibody, or antigen binding fragment, of any preceding claim that further inhibits the alternative complement pathway as measured by an in vitro hemolytic assay with an IC50 of less than or equal to 25 nM.
9. The isolated antibody, or antigen binding fragment, of any preceding claim, wherein said antibody binds Human Factor P and inhibits the alternative complement pathway as measured by an in vitro hemolytic assay with an IC50 of less than or equal to 16 nM
10. The isolated antibody, or antigen binding fragment thereof, of any preceding claim that further inhibits the alternative complement pathway as measure by an in vitro C3b deposition assay with an IC50 of less than or equal to 10 nM.
1 1. The antibody, or antigen binding fragment of claim 10, wherein said antibody binds Human Factor P and inhibits the alternative complement pathway as measured by an in vitro C3b deposition assay with an IC50 of less than or equal to 3 nM.
12. The isolated antibody, or antigen binding fragment of any preceding claim, that further inhibits the alternative complement pathway as measure by an in vitro MAC deposition assay with an IC50 of less than or equal to 25 nM.
13. The antibody, or antigen binding fragment of any preceding claim, wherein said antibody binds Human Factor P and inhibits the alternative complement pathway as measure by an in vitro MAC deposition assay with an IC50 of less than or equal to 25 nM.
14. The isolated antibody, or antigen binding fragment, of any preceding claim that further inhibits the alternative complement pathway as measured by an in vitro hemolytic assay with an IC50 of less than or equal to 25 nM, an in vitro C3b deposition assay with an IC50 of less than or equal to 10 nM and an in vitro MAC deposition assay with an IC50 of less than or equal to 25 nM.
15. An isolated antibody, or antigen binding fragment thereof, that binds Factor P and competes with an antibody described in Table 1.
16. An isolated antibody, or antigen binding fragment, that binds Factor P, said antibody or antigen binding fragment comprising: a) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 , 2, and 3, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6, respectively; b) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 15, 16, and 17, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 18, 19, and 20, respectively; c) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 29, 30, and 31 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 32, 33, and 34, respectively; d) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 43, 44, and 45, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 46, 47, and 48, respectively; e) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 57, 58, and 59, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 60, 61 , and 62, respectively; f) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 71 , 72, and 73, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 74, 75, and 76, respectively; g) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 85, 86, and 87, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 88, 89, and 90, respectively; h) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 99, 100, and 101 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 102, 103, and 104, respectively; i) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 13, 1 14, and 1 15, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 1 16, 1 17, and 1 18, respectively; j) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 127, 128, and 129, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 130, 131 , and 132, respectively; k) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 141 , 142, and 143, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 144, 145, and 146, respectively;
I) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 155, 156, and 157, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 158, 159, and 160, respectively; m) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 169, 170, and 171 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 172, 173, and 174, respectively; n) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 183, 184, and 185, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 186, 187, and 188, respectively; o) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 197, 198, and 199, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 200, 201 , and 202, respectively; p) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 21 1 , 212, and 213, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 214, 215, and 216, respectively; q) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 225, 226, and 227, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 228, 229, and 230, respectively; r) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 239, 240, and 241 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 242, 243, and 244, respectively; s) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 253, 254, and 255, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 256, 257, and 258, respectively; or t) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 267, 268, and 269, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 270, 271 , and 272, respectively.
17. The antibody, or antigen binding fragment, of any preceding claim comprising heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 7 and 8; SEQ ID NOs: 21 and 22; SEQ ID NOs: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID NOs: 105 and 106; SEQ ID NOs: 1 19 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID NOs: 161 and 162; SEQ ID NOs: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID NOs: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID NOs: 231 and 232; SEQ ID NOs: 245 and 246; SEQ ID NOs: 259 and 260; or SEQ ID NOs: 273 and 274, respectively.
18. An isolated antibody, or antigen binding fragment, which comprises a heavy chain variable region comprising SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203, 217, 231 , 245, 259, or 273 and further comprising a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to Factor P.
19. An isolated antibody, or antigen binding fragment, which comprises a light chain variable domain comprising SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274 and further comprising a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to Factor P.
20. The isolated antibody, or antigen binding fragment, of any preceding claim
wherein said light chain variable domain region comprises SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274.
21. An isolated antibody, or antigen binding fragment, which comprises a heavy chain of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further comprising a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to Factor P.
22. An isolated antibody, or antigen binding fragment, which comprises a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and further comprising a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to Factor P.
23. The isolated antibody, or antigen binding fragment, of any preceding claim
wherein said light chain comprises SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276.
24. An isolated antibody, or antigen binding fragment, that binds Factor P
comprising a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further comprising a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276.
25. An isolated antibody, or antigen binding fragment, that binds Factor P
comprising a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52, 65 and 66, 79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276.
26. The antibody or antigen binding fragment of any preceding claim, wherein said antibody is a human antibody, a chimeric antibody, a monoclonal antibody, a single chain antibody, Fab, Fab', F(ab')2, Fv or scFv.
27. The antibody or antigen binding fragment of any preceding claim, wherein said antibody is an IgG isotype.
28. An isolated nucleic acid molecule comprising a nucleotide encoding the
antibody or fragment of any preceding claim.
29. An isolated nucleic acid molecule encoding a polypeptide comprising the heavy chain variable region of any preceding claim.
30. The nucleic acid molecule of any preceding claim, wherein said nucleic acid has at least 95% sequence identity to a sequence selected from SEQ ID NOs: 1 1 , 25, 39, 53, 67, 81 , 95, 109, 123, 137, 151 , 165, 179, 193, 207, 221 , 235, 249, 263, and 277.
31. An isolated nucleic acid molecule encoding a polypeptide comprising the light chain variable region of any preceding claim.
32. The nucleic acid molecule of any preceding claim wherein said nucleic acid sequence has 95% sequence identity to SEQ ID NO: 12, 26, 40, 54, 68, 82, 96,
1 10, 124, 138, 152, 166, 180, 194, 208, 222, 236, 250, 264, and 278.
33. An isolated nucleic acid molecule encoding a polypeptide comprising the heavy chain of any preceding claim.
34. The nucleic acid molecule of any preceding claim wherein said nucleic acid sequence has 95% sequence identity to SEQ ID NO: 13, 27, 41 , 55, 69, 83, 97,
1 1 1 , 125, 139, 153, 167, 181 , 195, 209, 223, 237, 251 , 265 and 279.
35. An isolated nucleic acid molecule encoding a polypeptide comprising the light chain of any preceding claim.
36. The nucleic acid molecule of any preceding claim wherein said nucleic acid sequence has 95% sequence identity to SEQ ID NO: 14, 28, 42, 56, 70, 84, 98,
1 12, 126, 140, 154, 168, 182, 196, 210, 224, 238, 252, 266 and 280.
37. A vector comprising the nucleic acid molecule of any one of claims 28-36.
38. An isolated host cell comprising the vector of claim 37.
39. A composition comprising the antibody or antigen binding fragment of any
preceding claim and a pharmaceutically acceptable diluent or carrier.
40. A method of treating age related macular degeneration in a subject comprising administering to said subject, an effective amount of a composition comprising the antibody or antigen binding fragment of any preceding claim.
41. The method of claim 40 wherein the subject is human.
42. A method of inhibiting the alternative complement pathway in a subject
comprising administering to said subject an effective amount of a composition comprising the antibody or antigen binding fragment of any preceding claim.
43. The method of claim 42 wherein the subject is human.
44. A method of inhibiting complement mediated cell death, comprising contacting a cell with a composition comprising the antibody or antigen binding fragment of any preceding claim.
45. A method of inhibiting the formation of C3b in a cell, comprising contacting a cell with a composition comprising the antibody or antigen binding fragment of any preceding claim.
46. A method of inhibiting the formation of the Membrane Attack Complex in a cell, comprising contacting a cell with a composition comprising the antibody or antigen binding fragment of any preceding claim.
47. A method of inhibiting the alternative complement pathway in a cell, comprising contacting a cell with a composition comprising the antibody or antigen binding fragment of any preceding claim and measuring said pathway activity by an in vitro hemolytic assay, an in vitro C3b deposition assay, or an in vitro MAC deposition assay, wherein a decrease in pathway activity is measured by a 10% decrease in hemolysis, C3b deposition and/or MAC deposition.
48. A composition comprising a first antibody, or antigen binding fragment thereof, that binds Factor P, and a second antibody, or antigen binding fragment thereof, that binds C5, wherein said combination inhibits the alternative complement pathway.
49. The composition of claim 48, wherein said combination inhibits ocular
inflammation.
50. The composition of any of claims 48-49, wherein said ocular inflammation is determined by measuring neutrophil accumulation and/or macrophage recruitment in the retina.
51. The combination of any of claims 48-50, wherein said combination inhibits
neutrophil accumulation in the retina.
52. The combination of any of claims 48-51 , wherein said combination inhibits
macrophage recruitment in the retina.
53. The composition of any of claims 48-52, wherein said antibody that binds
Factor P, binds a region of Factor P comprising SEQ ID NO: 408.
54. The composition of any of claims 48-52, wherein said antibody that binds Factor P, binds a region of Factor P comprising SEQ ID NO: 407.
55. The composition of any of claims 48-54, wherein said first antibody, or antigen binding fragment thereof, is an antibody selected from Table 1 and said second antibody or antigen-binding fragment thereof is an antibody or antigen binding fragment selected from Table 2.
56. The composition of any of claims 48-55, wherein the first antibody, or antigen binding fragment thereof binds the same epitope as is an antibody described in Table 1 and the second antibody, or antigen binding fragment thereof, binds the same epitope as is an antibody described in Table 2.
57. The composition of any of claims 48-56 wherein the first antibody, or antigen binding fragment thereof comprises a heavy chain CDR1 , 2, 3, and a light chain CDR1 , 2, 3, selected from the group consisting of: a) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 , 2, and 3, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6, respectively; b) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 15, 16, and 17, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 18, 19, and 20, respectively; c) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 29, 30, and 31 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 32, 33, and 34, respectively; d) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 43, 44, and 45, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 46, 47, and 48, respectively; e) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 57,
58, and 59, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 60, 61 , and 62, respectively; f) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 71 , 72, and 73, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 74, 75, and 76, respectively; g) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 85, 86, and 87, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 88, 89, and 90, respectively; h) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 99, 100, and 101 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 102, 103, and 104, respectively; i) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1 13, 1 14, and 1 15, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 1 16, 1 17, and 1 18, respectively; j) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 127, 128, and 129, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 130, 131 , and 132, respectively; k) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 141 , 142, and 143, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 144, 145, and 146, respectively;
I) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 155, 156, and 157, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 158, 159, and 160, respectively; m) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 169, 170, and 171 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 172, 173, and 174, respectively; n) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 183, 184, and 185, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 186, 187, and 188, respectively; o) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 197, 198, and 199, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 200, 201 , and 202, respectively; p) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 21 1 , 212, and 213, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 214, 215, and 216, respectively; q) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 225, 226, and 227, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 228, 229, and 230, respectively; r) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 239, 240, and 241 , respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 242, 243, and 244, respectively; s) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 253, 254, and 255, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 256, 257, and 258, respectively; and t) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 267, 268, and 269, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 270, 271 , and 272, respectively and wherein the second antibody or antigen binding fragment thereof comprises a heavy chain CDR1 , 2, 3 and light chain CDR1 , 2, 3 selected from the group consisting of: a) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 410, 41 1 , and 412, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 413, 414, and 415, respectively; b) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 426, 427, and 428, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 429, 430, and 431 , respectively; c) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 442, 443, and 444, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 445, 446, and 447, respectively; d) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 426, 458, and 428, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 429, 430, and 459, respectively; and e) heavy chain variable region HCDR1 , HCDR2 and HCDR3 as set forth in SEQ ID NOs: 470, 471 , and 472, respectively, and light chain variable region LCDR1 , LCDR2, and LCDR3 as set forth in SEQ ID NOs: 473, 474 and 475, respectively.
The composition of any of claims 48-57, wherein the first antibody or antigen binding fragment thereof comprises heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 7 and 8; SEQ ID NOs: 21 and 22; SEQ ID NOs: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID NOs: 105 and 106; SEQ ID NOs: 1 19 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID NOs: 161 and 162; SEQ ID NOs: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID NOs: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID NOs: 231 and 232; SEQ ID NOs: 245 and 246; SEQ ID NOs: 259 and 260; or SEQ ID NOs: 273 and 274, respectively, and wherein the second antibody or antigen binding fragment thereof comprises heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 416 and 417; SEQ ID NOs: 432 and 433; SEQ ID NOs: 448 and 449; SEQ ID NOs: 460 and 461 ; or SEQ ID NOs: 476 and 477, respectively.
59. The composition of any of claims 48-58 wherein (a) the first antibody, or antigen binding fragment thereo comprises a heavy chain variable region comprising SEQ ID NO: 7, 21 , 35, 49, 63, 77, 91 , 105, 1 19, 133, 147, 161 , 175, 189, 203,
217, 231 , 245, 259, or 273 and further comprises a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof comprises a heavy chain variable region comprising SEQ ID NO: 416, 432, 448, 460 or 476 and further comprises a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an antigen binding site to C5.
60. The composition of any of claims 48-59, wherein the first antibody, or antigen binding fragment thereof (a) comprises a light chain variable domain comprising SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204,
218, 232, 246, 260, or 274 and further comprises a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof comprises a light chain variable region comprises a light chain variable domain comprising SEQ ID NO: 417, 433, 449, 461 or 477 and further comprises a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to C5.
61. The composition of claim 59, wherein the first antibody or antigen binding
fragment thereof comprises the light chain variable region sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274, and wherein the second antibody or antigen binding fragment thereof comprises the light chain variable region sequence of SEQ ID NO: 417, 433, 449, 461 or 477.
62. The composition of any of claims 48-61 wherein (a) the first antibody, or antigen binding fragment thereof comprises a heavy chain of SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further comprises a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof comprises a heavy chain of SEQ ID NO: 418, 434, 450, 462, or 478 and further comprises a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to C5.
63. The composition of any of claims 48-62, wherein (a) the first antibody, or
antigen binding fragment thereof comprises a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and further comprises a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof comprises a light chain of SEQ ID NO: 419, 435, 451 , 463, or 479 and further comprises a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to C5.
64. The composition of claim 62, wherein the first antibody or antigen binding
fragment thereof comprises a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276, and wherein the second antibody or antigen binding fragment thereof comprises a light chain of SEQ ID NO: 419, 435, 451 , 463, or 479.
65. The composition of claim 48-64, wherein the first antibody, or antigen binding fragment thereof comprises a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 9, 23, 37, 51 , 65, 79, 93, 107, 121 , 135, 149, 163, 177, 191 , 205, 219, 233, 247, 261 or 275 and further comprises a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second antibody or antigen binding fragement thereof comprises a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 418, 434, 450, 462, or 478 and further comprises a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 419, 435, 451 , 463, or 479.
66. The composition of any of claims 48-65, wherein the first antibody, or antigen binding fragment thereof comprises a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity, respectively, to SEQ ID NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52, 65 and 66, 79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment thereof comprises a heavy chain and a light chain with an amino acid sequence having at least 90% sequence identity, respectively, to SEQ ID NOs: 418 and 419, 434 and 435; 450 and 451 ; 462 and 463; or 478 and 479.
67. An isolated nucleic acid molecule comprising a nucleotide sequence encoding the first antibody or fragment of any of claims 48-66.
68. An isolated nucleic acid molecule comprising nucleotide sequence encoding the second antibody or antigen binding fragment thereof of any of claims 48-66.
69. A vector comprising the nucleic acid molecule of claim 67 or 68
70. An isolated host cell comprising the vector of claim 69.
71. A method of treating age related macular degeneration in a subject comprising administering to said subject, an effective amount of the composition of any of claims 48-66.
72. The method of claim 71 wherein the subject is human.
73. A method of inhibiting the alternative complement pathway in a subject
comprising administering to said subject an effective amount of the composition of any of claims 48-66.
74. The method of claim 73, wherein said subject is human.
75. The antibody or antigen binding fragment thereof of claim 1 for use as a
medicament.
76. The composition of claim 48 for use as a medicament.
77. The antibody or antigen binding fragment thereof of any of claims 1-27 for use in the treatment of age related macular degeneration.
78. The antibody or antigen binding fragment thereof of any of claims 1-27 for use in the inhibition of the alternative complement pathway.
79. The antibody or antigen binding fragment thereof of any of claims 1-27 for use in the inhibition of complement mediated cell death.
80. The antibody or antigen binding fragment thereof of any of claims 1-27 for use in the inhibition of the formation of C3b in a cell.
81. The antibody or antigen binding fragment thereof of any of claims 1-27 for use in the inhibition of the formation of the Membrane Attack Complex in a cell.
82 The composition of any of claims 48-66 for use in the treatment of age related macular degeneration.
83. The composition of any of claims 48-66 for use in the inhibition of the
alternative complement pathway in a subject.
84. A method of treating age related macular degeneration in a subject comprising administering to said subject, an effective amount of the composition of claim 48.
85. The method of claim 84 wherein the subject is human.
86. A method of inhibiting the alternative complement pathway in a subject
comprising administering to said subject an effective amount of the composition of claim 48.
87. The method of claim 86, wherein said subject is human.
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US15/973,225 US10865237B2 (en) 2011-12-21 2018-05-07 Nucleic acids encoding anti-Factor P antibodies
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