NZ626296B2 - Compositions and methods for antibodies targeting factor p - Google Patents

Abstract

The present invention relates to antibodies or antigen binding fragments thereof that bind to complement Factor P (also known as Properdin).

Description

COMPOSITIONS AND METHODS FOR ANTIBODIES TARGETING FACTOR P BACKGROUND OF THE INVENTION Age related r degeneration (AMD) is a progressive disease and a leading cause of vision loss and blindness in Americans aged 65 and older. AMD primarily affects the macula; a part of the retina sible for high visual acuity needed to read or drive. The majority of AMD patients suffer from an early stage of the e which is characterized by the presence of extracellular retinal deposits called drusen. Drusen are extracellular retinal deposits of cell debris, inflammatory mediators, and extracellular matrix components. The late stages of AMD manifest as a dry or wet form, both are associated with vision loss. Dry AMD, also known as geographic atrophy, appears on lmoscopic ation as clearly demarcated regions corresponding to local areas of retinal pigmented epithelium (RPE) loss. Wet AMD is associated with neo— vascularization of the choriod, causing a loss of integrity in s membrane and vessel growth in the retina, where they can often hemorrhage. This leakage causes permanent damage to retinal cells which die off and create blind spots in the central vision.

The innate human system is composed of the complement pathway. The complement pathway serves to defend against ic bacterial infection bridging innate and adaptive immunity; and disposing of products of immune complexes and inflammatory injury. The ment is a system of more than 30 proteins involved in cascade reactions in plasma and cell surfaces. The complement system and its complement components are involved in various immune processes. For example, complement C5b-9 complex, also termed the al complex or the ne attack complex (MAC), plays an important role in cell death by inducing membrane permeability damages.

There are three known ment activation ys: the classical, lectin, and alternative pathways. All three ys lead to the cleavage of C3 by C3 convertase and subsequent cleavage of C5 by the CS convertase, releasing C3a, C5a, and 05b.

Factor P is a key regulator of the alternative complement pathway. it is proposed to have two major functions in vivo. First, Factor P stabilizes the C3 and 05 convertases by binding to 03b of the convertase enzyme and thereby prolongs the half life of C3 convertase. Second, Factor P may determine which cells will be lysed by attaching to a cell surface and functioning as a template on which convertases can form, leading to activation of the alternative ment pathway and lysis of the cell.

Recent work has demonstrated that complement components C3 and CS are principal constituents of drusen in patients with AMD. Mulling, R.F. et al. (2000) FASEB J 14, 835-46 Their presence as well as that of the membrane attack complex (MAC) C5b-9 and other acute phase reactant proteins in RPE cells overlying drusen has been speculated to be involved in the process that can trigger complement activation and formation of MAC. Johnson, L et al. (2001) Exp Eye Res 73, 887-896. Thus, there is growing evidence that ment components are more than mere mediators of innate immunity.

Nutritional intervention has been prescribed to inhibit ssion of dry AMD to wet AMD. At present the only FDA approved treatments for wet AMD include photodynamic therapy (PDT), an EGF aptamer, such as pegaptanib, and anti- VEGF antibodies, ranibizumab. These drugs or ies are typically administered to patients who have already suffered ntial vision loss.

There remains a need to develop an effective ent for AMD, particularly dry AMD to replace or supplement current ents. Particularly, there is a need for treatments which can provide early detection, prevention or restoration of vision loss.

SUMMARY OF THE INVENTION The present invention and the ion of NZ 721643, which was divided from the present application, relate to an isolated dy, or antigen binding fragment thereof, that binds to human or cynomolgus Factor P, wherein said antibody binds to the TSR5 domain (SEQ ID NO: 406). For example, the antibodies, or antigen binding fragments described herein bind to a region of the TSR5 domain comprising the sequence of SEQ ID NO: 407, more specifically said antibodies also bind a region of the Factor P TSR5 domain comprising the amino acid sequence KSlSC (SEQ ID NO: 408).

In certain embodiments, the isolated antibodies, or antigen binding fragments thereof, bind to a Factor P epitope comprising the amino acid sequence of SEQ ID NO: 407. In other ments, the isolated antibodies, or antigen binding fragments thereof, bind to a Factor P epitope sing the amino acid sequence of SEQ ID NO: 408.

In a first aspect, the t invention es an isolated antibody, or antigen binding fragment, that binds Factor P, said antibody or antigen binding fragment comprising: a) heavy chain variable region HCDRt, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain variable region LCDRt, LCDRZ, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6, respectively; (followed by page 2a) b) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 85, 86, and 87, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 88, 89, and 90, respectively; c) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 99, 100, and 101, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N05: 102, 103, and 104, respectively; d) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 113, 114, and 115, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 116, 117, and 118, respectively; 1O e) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N05: 169, 170, and 171, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N05: 172, 173, and 174, respectively; f) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 183, 184, and 185, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 186, 187, and 188, respectively; 9) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N05: 197, 198, and 199, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 200, 201, and 202, respectively; h) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 211, 212, and 213, tively, and light chain le region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 214, 215, and 216, respectively; or i) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 225, 226, and 227, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 228, 229, and 230, tively.

The isolated dies, or antigen binding fragments, described herein bind Factor P, with a KD of less than or equal to 1.2 nM. For example, the isolated antibodies or antigen binding fragments described herein may bind to human or cynomolgus Factor P with a KD of less than or equal to 1.1 nM, less than or equal to 1nM, less than or equal to 600pM, less than or equal to 500 pM, less than or equal to 400 pM, less than or equal to 300 pM, less than or equal to 200 pM, less than or equal to 100 pM, less than or equal [FOLLOWED BY PAGE 3] PCT/IBZOlZ/057394 to 75 pM, less than or equal to 50 pM, less than or equal to 40pM, less than or equal to pM, less than or equal to 20 pM, or less than or equal to 10pM.

The binding affinity of isolated antibodies and antigen binding nts described herein can be determined by solution equilibrium titration (SET). Methods for SET are known in the art and are bed in further detail below. Alternatively, binding y of the isolated antibodies, or fragments, described herein can be determined by Biacore assay. Methods for Biacore kinetic assays are know in the art and are described in further detail below.

The isolated antibodies and n binding fragments described herein can be used to inhibit the alternative complement pathway. For example, an isolated antibody or antigen binding fragment thereof can inhibit the alternative complement pathway as e by an in vitro hemolytic assay with an lCSO of less than or equal to 25 nm, less than or equal to 20 nM, less than or equal to 16nM, less than or equal to 15nM, less than or equal to 14nM, less than or equal to 13nM, less than or equal to 12nM, less than or equal to 11nM, less than or equal to 10nM, less than or equal to 9nM, less than or equal to 8nM, less than or equal to 7nM. More specifically, an isolated antibody or antigen g fragment thereof as bed herein can inhibit the alternative complement y in human as measure by an in vitro hemolytic assay with an ICSO of less than or equal to 16 nm, or less than or equal to 9 nm.

An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway as measure by an in vitro 03b deposition assay with an |C50 of less than or equal to 10 nm, less than or equal to 7nM, less than or equal to 6 nM, less than or equal to 5nM, less than or equal to 4 nM, less than or equal to 3 nM, less than or equal to 2 nM, less than or equal to 1 nM, less than or equal to 15nM, less than or equal to 1 nM, less than or equal to 0.5 nM, or less than or equal to 0.1 nM. More ically, an isolated antibody or antigen binding fragment thereof as bed herein can inhibit the alternative ment pathway in human as measure by an in vitro C3b deposition assay with an ICSO of less than or equal to 3 nm, or less than or equal to 2 nM.

An isolated antibody or antigen binding fragment thereof as described herein can inhibit the alternative complement pathway with an ICSO of less than or equal to 25 nm, less than or equal to 20 nM, less than or equal to 15 nM, less than or equal to 10 nM, less than or equal to 9 nM, less than or equal to 8 nM, less than or equal to 7 nM, or less than or equal to 6 nM, as measure by deposition of the complement membrane attack complex. More specifically, an isolated antibody or fragment thereof as described herein can inhibit the alternative complement pathway in human with an ICSO of less than or equal to 25 nm, or less than or equal to 7.5 nM, as measure by deposition of the ment membrane attack complex.

An isolated antibody or antigen binding fragment thereof as described herein can inhibit the ative ment y with an lCSO of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of 03a.

An isolated antibody or antigen binding fragment thereof as bed herein may also inhibit the alternative complement pathway with an lCSO of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of iCSb.

An isolated antibody or antigen g fragment f as described herein may also t the ative complement pathway with an ICSO of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C5a.

An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway with an ICSO of less than or equal to 80nM, less than or equal to 50nM, less than or equal to 45nM, or less than or equal to 35nM, as measure by generation of C5b.

An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the alternative complement pathway by destabilizing and/or blocking the activity of C3 and/or CS convertase, as measured by a decrease in production of C3a, 03b, iC3b, 05a, and/or C5b.

An isolated antibody or antigen binding fragment thereof as described herein may also inhibit the generation of 05a with an ICSO of less than or equal to 80nM, less than or equal to SOnM, less than or equal to 45nM, or less than or equal to 35nM.

The isolated antibodies, or antigen g fragment thereof, may also block Factor P binding to 03b and/or prevent Factor P binding to the cell surface or to DNA or oligonucleotides.

Another aspect of the invention includes an isolated antibody, or n binding fragment thereof, that specifically binds to human, cynomolgus, rat and/or rabbit Factor P. In a further aspect, the isolated antibody, or antigen binding fragment, competes for g with an antibody, or antigen binding fragment, described in Table 1.

The isolated antibodies, or antigen binding fragments thereof, as described herein can be a monoclonal antibodies, a human or zed antibodies, a chimeric antibodies, single chain antibodies, Fab fragments, Fv fragments, F(ab’)2 fragments, or ScFv nts, and/or IgG isotypes.

The isolated antibodies, or antigen binding fragments thereof, as described herein can also include a framework in which an amino acid has been tuted into the antibody framework from the respective human VH or VL germline sequences.

Another aspect of the invention includes an isolated antibody or antigen binding fragment thereof having the heavy and light chain sequences of Fabs described in Table 1. For e, the isolated antibody or antigen binding fragment thereof can have the heavy and light chain sequences of Fab NV8962, NV8963, NVS964, NVS965, , , NV8962-G, NV8962-S, NVS962-T, NVS962-Q, NVS962-SB1A, NV8965-Q, NVS965—S, NVS965—T, NV8804, , NVS806, NV8807, or NV8808.

A further aspect of the invention includes an isolated dy or antigen binding nt thereof having the heavy and light chain variable domain sequences of Fabs described in Table 1. For example, the isolated dy or antigen binding fragment there of can have the heavy and light chain variable domain sequence of Fab NV8962 , NVS963, NVS964, NV8965, NV8966, NVSQ67, NV8962—G, NVS962—S, NV8962-T, NV8962-Q, NV8962-S31A, NVS965—Q, NVS965-S, NV8965—T, NVS804, , NVS806, NVS807, or NVS808.

The ion also relates to an isolated antibody or antigen binding fragment thereof that includes a heavy chain CDR1 selected from the group ting of SEQ lD NOs 1, 15, 29, 43, 57, 71, 85, 99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, and 267; a heavy chain CDR2 selected from the group consisting of SEQ ID NOs: 2, 16, , 44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268; and a heavy chain CDR3 selected from the group consisting of SEQ lD NOs: 3, 17, 31, 45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199, 213, 227, 241, 255, and 269, wherein the isolated antibody or antigen binding fragment thereof binds to human Factor P. in another , the isolated antibody or antigen binding fragment thereof further includes a light chain CDR1 selected from the group ting of SEQ lD NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186,200,214, 228, 242, 256, and 270; a light chain CDR2 selected from the group consisting of SEQ ID NOs 5, 19, 33, 47, 61, PCT/IBZOlZ/057394 75,89, 103, 117, 131, 145, 159, 173, 187,201, 215, 229,243,257, and 271; and a light chain CDR3 selected from the group consisting of SEQ ID NOs 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188,202, 216, 230, 244, 258, and 272.

The invention also relates to an isolated dy or antigen binding fragment thereof that includes a light chain CDR1 ed from the group consisting of SEQ ID NOs: 4, 18, 32,46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; a light chain CDR2 selected from the group consisting of SEQ ID NOs 5, 19, 33,47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201,215, 229,243, 257, and 271; and a light chain CDR3 selected from the group ting of SEQ ID NOs 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188,202, 216, 230, 244, 258, and 272, wherein the isolated antibody or antigen binding fragment thereof binds to human Factor The invention also relates to an ed dy or antigen binding nt thereof that binds Factor P having HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs: 1, 2, 3, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 4, 5, 6; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N08 15, 16, 17, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 18, 19, 20; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ lD N03 29, 30, 31, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N08: 32, 33, 34; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N08 43, 44, 45, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 46, 47, 48; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N03 57, 58, 59, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N05: 60, 61, 62; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ lD N03 71, 72, 73, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 74, 75, 76; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 85, 86, 87, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 88, 89, 90; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ lD N03 99, 100, 101, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 102, 103, 104; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N03 113, 114, 115, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N08: 116, 117, 118; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N08 127, 128, 129, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N08: 130, 131, 132; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N05 141, 142, 143, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 144, 145, 146; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N08 155, 156, 157, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 158, 159, 160; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N05 169, 170, 171, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 172, 173, 174; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N03 183, 184, 185, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 186, 187, 188; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, n HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 197, 198, 199, and LCDR1, LCDR2, LCDR3 ses SEQ ID NOs: 200, 201, 202; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 211, 212, 213, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 214, 215, 216; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 225, 226, 227, and LCDR1, LCDR2, LCDR3 comprises SEQ ID NOs: 228, 229, 230; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID NOs 239, 240, 241, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N05: 242, 243, 244; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, n HCDR1, HCDR2, HCDR3 comprises SEQ ID N03 253, 254, 255, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N08: 256, 257, 258; or HCDR1, HCDR2, HCDR3 and LCDR1, LCDR2, LCDR3, wherein HCDR1, HCDR2, HCDR3 comprises SEQ ID N03 267, 268, 269, and LCDR1, LCDR2, LCDR3 comprises SEQ ID N03: 270, 271, 272.

In one embodiment of the invention the ed antibody or n binding fragment thereof includes a heavy chain variable domain sequence selected from the group consisting of SEQ ID NOs: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 and 273. In another embodiment , the isolated antibody or antigen binding fragment further comprises a light chain variable domain seqeunce wherein the heavy chain le domain and light chain variable domain combine to form and antigen binding site for Factor P. In a r embodiment the isolated antibody or antigen binding fragment further includes a light chain variable domain sequence selected from SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274 wherein said isolated dy or antigen binding fragment thereof binds Factor P.

The invention also relates to an isolated antibody or antigen g fragment thereof that includes a light chain variable domain sequence selected from the group consisting of SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, wherein said isolated antibody or antigen binding fragment thereof binds to human Factor P. In one embodiment or , the isolated antibody antigen g fragment further comprises a heavy chain le domain sequence wherein the light chain variable domain and heavy chain variable domain combine to form and antigen binding site for Factor P.

In another embodiment of the invention, the isolated antibody or antigen binding nt thereof that binds Factor P, may have heavy and light chain variable domains comprising the sequences of SEQ lD NOs: 7 and 8; 21 and 22; 35 and 36; 49 and 50; 63 and 64; 77 and 78; 91 and 92; 104 and 105; 118 and 119; 132 and 133; 146 and 147; 160 and 161; 174 and 175; 188 and 189; 202 and 203; 216 and 217; 230 and 231; 244 and 245; 258 and 259; or 272 and 273, respectively.

The invention further relates to an isolated antibody or antigen g nt thereof, that includes a heavy chain variable domain having at least 90% sequence identity to a sequence selected from the group ting of SEQ ID NOs: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 and 273, wherein said antibody binds to Factor P. in one aspect, the isolated antibody or antigen binding fragment thereof also includes a light chain variable domain having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274.

In another embodiment the isolated antibody or antigen binding fragment thereof, may include a light chain variable domain having at least 90% sequence ty to a sequence selected from the group consisting of SEQ lD NOS 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232,246, 260, and 274, wherein said antibody binds Factor P.

In another embodiment the isolated antibody, or n binding fragment thereof, that binds to Factor P may have a heavy chain sing the ce of SEQ lD NO: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261 or 275. in a further embodiment, the isolated antibody also es a light chain that can combine with the heavy chain to form an antigen binding site to human Factor P. in a further embodiment, the isolated antibody or antigen binding fragment thereof es a light chain having a sequence comprising SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276.

The invention still further relates to an isolated antibody or antigen binding nt thereof that includes a heavy chain having at least 90% sequence ty to a sequence selected from the group consisting of SEQ lD NOs 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191,205, 219, 233, 247, 261 and 275, wherein said antibody binds to Factor P. In one aspect, the isolated antibody or antigen binding fragment thereof also includes a light chain having at least 95% sequence identity to a sequence selected from the group consisting of SEQ lD N08 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192,206, 220, 234,248, 262, and 276.

The invention still further s to an isolated antibody or antigen g fragment thereof that includes a light chain having at least 90% sequence identity to a sequence selected from the group ting of SEQ lD NOs 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247,261 and 275, wherein said antibody binds Factor P.

The ion also relates to compositions comprising the isolated antibody, or n g fragment thereof, described herein. As well as, antibody compositions in combination with a pharmaceutically acceptable carrier. Specifically, the invention further includes pharmaceutical compositions comprising an antibody or antigen binding fragment thereof of Table 1, such as, for example antibody NVS962, NV8963, NV8964, NV8965, NVS966, NV8967, NV8962-G, NV8962-S, NV8962-T, NVS962—Q, NVS962— 831A, NVS965—Q, NV8965-S, —T, NV8804, NV8805, NVS806, NV8807, or NVSBO8. The invention also realtes to pharmaceutical compositions comprising a combination of two or more of the isolated antibodies or antigen binding fragments thereof of Table 1.

The invention also relates to an ed nucleic acid comprising a ce encoding a polypeptide that includes a heavy chain le domain having at least 90% ce identity to a sequence selected from the group ting of SEQ ID NOs: 7, 21, 35, 49, 63, 77, 91, 105,119,133,147,161, 175, 189, 203, 217, 231, 245, 259 and 273.

The invention also relates to an isolated nucleic acid comprising a sequence encoding a polypeptide that includes a light chain variable domain having at least 90% sequence identity to a sequence selected from the group consisting of SEQ ID NOs 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274.

The invention also s to a vector that includes one or more of the nucleic acid molecules described herein.

The invention also relates to an isolated host cell that includes a inant DNA sequence encoding a heavy chain of the antibody described above, and a second recombinant DNA sequence encoding a light chain of the antibody described above, wherein said DNA sequences are operably linked to a promoter and are capable of being expressed in the host cell. it is plated that the antibody can be a human monoclonal antibody. it is also contemplated that the host cell is a non—human mammalian cell.

The invention also s to a method of inhibiting the ment mediated cell death wherein the method includes the step of contacting a cell with an effective amount of a composition comprising the isolated antibody or n binding fragments thereof described herein. It is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. it is still further contemplated that the subject is human.

The invention still further relates to a method of ting the alternative complement pathway in a cell wherein the method includes the step of contacting the cell with an ive amount of a composition comprising the isolated antibody or n binding fragments thereof described herein. In one aspect, it is contemplated that the cell is a human cell. It is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.

The invention also relates to a method of inhibiting the formation of membrane attack complex in a cell wherein the method includes the step of contacting the cell with an ive amount of a ition comprising the ed antibody or antigen binding fragments thereof described herein. it is contemplated that the cell is a human cell. it is further contemplated that the cell is in a subject. It is still further contemplated that the subject is human.

Any of the foregoing isolated antibodies or n binding fragments thereof may be a monoclonal antibody or antigen binding fragment thereof.

PCT/[32012/057394 In one aspect, the invention provides a a first antibody, or antigen binding fragment thereof, that binds Factor P, and a second antibody, or antigen binding fragment thereof, that binds C5, wherein said combination inhibits the alternative complement pathway. In one aspect the first and second antibodies can be in combination as a composition.

Such a combination can be used to inhibit ocular inflammation. Ocular inflammation can be determined by measuring neutrophil accumulation and/or macrophage recruitment in the retina.

In one aspect, such a combination can be used to inhibit neutrophil accumulation in the retina, or macrophage recruitment in the .

In one aspect, the antibody in such a combination that binds Factor P, binds a region of Factor P comprising SEQ ID NO: 408. Alternatively or in combination, such an antibody binds a region of Factor P comprising SEQ ID NO: 407.

In a further aspect, the combination of antibodies or binding fragments thereof that bind Factor P and 05 e a first antibody or antigen binding fragment selected from Table 1 and a second antibody or antigen-binding fragment selected from Table 2.

In one aspect, the first dy, or antigen binding nt thereof binds the same epitope as is an antibody described in Table 1 and the second dy, or antigen binding fragment thereof, binds the same e as is an antibody described in Table 2.

In one aspect, the invention provides a first antibody, or antigen binding fragment thereof that comprises a heavy chain CDR1, 2, 3, and a light chain CDR1, 2, 3, selected from the group ting of a) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6, tively; b) a heavy chain le region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 15, 16, and 17, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N08: 18, 19, and 20, respectively; 0) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 29, 30, and 31, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 32, 33, and 34, respectively; d) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 43, 44, and 45, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 46, 47, and 48, respectively; e) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 57, 58, and 59, tively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 60, 61, and 62, respectively; f) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 71, 72, and 73, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N05: 74, 75, and 76, respectively; 9) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 85, 86, and 87, tively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 88, 89, and 90, tively; h) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 99, 100, and 101, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 102, 103, and 104, respectively; i) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 113, 114, and 115, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 116, 117, and 118, respectively; j) a heavy chain le region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 127, 128, and 129, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOS: 130, 131, and 132, respectively; k) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 141, 142, and 143, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 144, 145, and 146, tively; I) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 155, 156, and 157, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 158, 159, and 160, respectively; m) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 169, 170, and 171, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 172, 173, and 174, respectively; n) a heavy chain le region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 183, 184, and 185, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 186, 187, and 188, respectively; 0) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 197, 198, and 199, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 200, 201, and 202, respectively; p) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 211, 212, and 213, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 214, 215, and 216, respectively; q) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 225, 226, and 227, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N08: 228, 229, and 230, respectively; r) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 239, 240, and 241, respectively, and light chain variable region PCT/[82012/057394 LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 242, 243, and 244, respectively; 5) a heavy chain le region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 253, 254, and 255, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 256, 257, and 258, respectively; and t) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N05: 267, 268, and 269, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N08: 270, 271, and 272, respectively, and wherein the second antibody or antigen binding nt thereof comprises a heavy chain CDR1, 2, 3 and light chain CDR1, 2, 3 selected from the group consisting of: a) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 410, 411, and 412, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N05: 413, 414, and 415, respectively; b) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 426, 427, and 428, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 429, 430, and 431, respectively; 0) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 442, 443, and 444, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 445, 446, and 447, respectively; d) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 426, 458, and 428, respectively, and light chain le region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 429, 430, and 459, respectively; and e) a heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 470, 471, and 472, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 473, 474 and 475, respectively.

In one aspect, the invention relates to a first and second dy or antigen bidning fragement thereof (which may be in combination as a ition) where the first antibody or n binding fragment thereof includes heavy and light chain variable regions having amino acid sequences at least 90% identical to SEQ ID NOs: 7 and 8; SEQ ID N03: 21 and 22; SEQ ID N03: 35 and 36; SEQ ID NOs: 49 and 50; SEQ ID NOs: 63 and 64; SEQ ID NOs: 77 and 78; SEQ ID NOs: 91 and 92; SEQ ID N03: 105 and 106; SEQ ID N03: 119 and 120; SEQ ID NOs: 133 and 134; SEQ ID NOs: 147 and 148; SEQ ID N03: 161 and 162; SEQ ID N03: 175 and 176; SEQ ID N08: 189 and 190; SEQ ID N08: 203 and 204; SEQ ID NOs: 217 and 218; SEQ ID N08: 231 and 232; SEQ ID N03: 245 and 246; SEQ ID N03: 259 and 260; or SEQ ID N08: 273 and 274, respectively, and wherein the second antibody or antigen binding nt thereof includes heavy and light chain variable regions having amino acid sequences at least PCT/IBZOIZ/057394 90% identical to SEQ ID N03: 416 and 417; SEQ ID N03: 432 and 433; SEQ ID N08: 448 and 449; SEQ ID N08: 460 and 461; or SEQ ID N08: 476 and 477, respectively.

In one aspect, the invention s to a first and second dy or antigen bidning fragement thereof (which may be in combination as a ition) where the first antibody or antigen binding fragment thereof includes heavy and light chain variable s having amino acid sequences selected from SEQ ID NOs: 7 and 8; SEQ ID N03: 21 and 22; SEQ ID N03: 35 and 36; SEQ ID N08: 49 and 50; SEQ ID N08: 63 and 64; SEQ ID N05: 77 and 78; SEQ ID N08: 91 and 92; SEQ ID N08: 105 and 106; SEQ ID N03: 119 and 120; SEQ ID N03: 133 and 134; SEQ ID NOS: 147 and 148; SEQ ID N05: 161 and 162; SEQ ID N05: 175 and 176; SEQ ID NOs: 189 and 190; SEQ ID N03: 203 and 204; SEQ ID N03: 217 and 218; SEQ ID N08: 231 and 232; SEQ ID NOs: 245 and 246; SEQ ID N03: 259 and 260; or SEQ ID N03: 273 and 274, respectively, and wherein the second antibody or antigen binding fragment thereof includes heavy and light chain variable s having amino acid sequences selected from SEQ ID N03: 416 and 417; SEQ ID N05: 432 and 433; SEQ ID N03: 448 and 449; SEQ ID N08: 460 and 461; or SEQ ID N03: 476 and 477, respectively.

In a further aspect, the invention includes a first and second dy or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first antibody, or antigen binding fragment thereo includes a heavy chain variable region comprising SEQ ID NO: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259, or 273 and further includes a light chain variable region, wherein said heavy chain variable region and said light chain variable region combine to form an n binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof es a heavy chain variable region comprising SEQ ID NO: 416, 432, 448, 460 or 476 and further includes a light chain variable region, wherein said heavy chain le region and said light chain variable region combine to form an antigen binding site to 05. In a further aspect, the first antibody or antigen binding nt thereof includes the light chain variable region sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274, and the second dy or antigen binding fragment thereof includes the light chain variable region sequence of SEQ ID NO: 417, 433, 449, 461 or 477.

In a further aspect, the invention includes a first and second antibody or antigen g fragment thereof (which may be in combination as a composition) in which (a) the first antibody or antigen bidning fragment thereof incldues a light chain variable domain comprising SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274 and further includes a heavy chain variable domain, wherein the light chain variable domain and the heavy chain le domain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a light chain variable region comprises a light chain variable domain es SEQ ID NO: 417, 433, 449, 461 or 477 and further comprises a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen g site to C5.

In one , the invention includes a first and second antibody or antigen binding fragment thereof (which may be in ation as a composition) in which (a) the first antibody, or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 9, 23, 37, 51, 65, 79, 93, 107, 121,135, 149,163, 177, 191, 205, 219, 233, 247, 261 or 275 and further includes a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a heavy chain of SEQ ID NO: 418, 434, 450, 462, or 478 and further includes a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to 05. In a r aspect, the first antibody or antigen binding fragment f includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276, and n the second antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 419, 435, 451, 463, or 479.

In one aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) in which (a) the first dy, or antigen binding fragment f includes a light chain of SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and further includes a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to Factor P and (b) wherein the second antibody or antigen binding fragment thereof includes a light chain of SEQ ID NO: 419, 435, 451, 463, or 479 and r includes a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to C5.

In one aspect, the ion includes a first and second antibody or antigen g fragment thereof (which may be in ation as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219,233,247, 261 or 275 and further includes a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second antibody or antigen binding fragement f es a heavy chain with an amino acid sequence having at least 90% sequence identity to SEQ lD NO: 418, 434, 450, 462, or 478 and further includes a light chain with an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 419, 435, 451, 463, or 479.

In a further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a ition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain with an amino acid sequence ted from SEQ lD NO: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219,233, 247, 261 or 275 and further includes a light chain with an amino acid sequence selected from SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 or 276 and wherein the second dy or antigen binding fragement thereof includes a heavy chain with an amino acid sequence ed from SEQ lD NO: 418, 434, 450, 462, or 478 and further includes a light chain with an amino acid sequence selected from SEQ ID NO: 419, 435, 451, 463, or 479. in a further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) n the first dy, or n binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence having at least 90% ce identity, respectively, to SEQ ID NO: 9 and 10,23 and 24, 37 and 38, 51 and 52, 65 and 66,79 and 80, 93 and 94,107 and108, 121 and 122, 135 and 136, 149 and150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence having at least 90% sequence ty, respectively, to SEQ lD N03: 418 and 419, 434 and 435; 450 and 451; 462 and 463; or 478 and 479. in a still further aspect, the invention includes a first and second antibody or antigen binding fragment thereof (which may be in combination as a composition) wherein the first antibody, or antigen binding fragment thereof includes a heavy chain and a light chain with an amino acid sequence, respectively, selected from SEQ lD NO: 9 and 10, 23 and 24, 37 and 38, 51 and 52,65 and 66,79 and 80, 93 and 94, 107 and 108, 121 and 122, 135 and 136, 149 and 150, 163 and 164, 177 and 178, 191 and 192, 205 and 206, 219 and 220, 233 and 234, 247 and 248, 261 and 262, or 275 and 276; and wherein the second antibody or antigen binding fragment f includes a heavy chain and a light chain with an amino acid sequence, respectively, selected from SEQ ID N03: 418 and 419, 434 and 435; 450 and 451; 462 and 463; or 478 and 479.

The invention further relates to an isolated nucleic acid le comprising a nucleotide ce ng the first and/or second antibody or antigen binding fragment f as described herein. Such a nucleic acid sequence can be included in a vector, which may, in turn be included in a host cell which, in one aspect, is e of expressing such c acid sequence.

The invention further relates to a method of treating age related macular degeneration in a subject comprising administering to said subject, an effective amount of a first and second antibody or antigen binding fragment thereof, either singly, or in combination as a composition. The t may be a human.

The invention further releates to a method of inhibiting the alternative complement pathway in a subject comprising administering to said subject an effective amount of a first and second antibody or antigen binding fragment thereof, either singly, or in combination as a composition. The subject may be a human.

DEFINITIONS Unless defined othenNise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this invention pertains.

The term "antibody" as used herein means a whole antibodies and any antigen binding fragment (1'. e., "antigen-binding portion") or single chains thereof. A whole dy is a glycoprotein comprising at least two heavy (H) chains and two light (L) chains inter-connected by disulfide bonds. Each heavy chain is comprised of a heavy chain le region (abbreviated herein as VH) and a heavy chain constant region.

The heavy chain constant region Is comprised of three domains, CH1, CH2 and CH3.

Each light chain is comprised of a light chain variable region (abbreviated herein as VL) and a light chain constant region. The light chain constant region is comprised of one domain, CL. The VH and VL regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR). Each VH and VL is composed of three CDRs and four FRs ed from amino-terminus to y— terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The variable regions of the heavy and light chains contain a binding domain that interacts with an n. The constant regions of the antibodies may mediate the binding of the immunoglobulin to host tissues or s, including various cells of the immune system (e.g., effector cells) and the first component (Clq) of the cal complement system.

The term "antigen binding portion" or “antigen binding fragment” of an antibody, as used herein, refers to one or more fragments of an intact antibody that retain the ability to specifically bind to a given n (e.g., Factor P). Antigen binding functions of an antibody can be performed by fragments of an intact antibody. Examples of binding nts encompassed within the term n binding portion or antigen binding fragment of an antibody include a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; a F(ab)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; an Fd fragment consisting of the VH and CH1 domains; an Fv fragment consisting of the VL and VH domains of a single arm of an dy; a single domain antibody (dAb) nt (Ward et al., 1989 Nature 341:544—546), which consists of a VH domain or a VL domain; and an isolated mentarity determining region (CDR).

Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by an artificial peptide linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv ; see, 6.9., Bird etal., 1988 Science 242:423-426; and Huston etal., 1988 Proc.

Natl. Acad. Sci. 85:5879-5883). Such single chain antibodies include one or more antigen binding ns or nts of an dy. These antibody fragments are obtained using conventional techniques known to those of skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.

Antigen binding fragments can also be incorporated into single domain antibodies, maxibodies, minibodies, intrabodies, diabodies, triabodies, tetrabodies, v— NAR and bis-scFv (see, e.g., Hollinger and Hudson, 2005, Nature Biotechnology, 23, 9, 1126-1136). Antigen binding portions of antibodies can be grafted into scaffolds based on polypeptides such as Fibronectin type lll (Fn3) (see US. Pat. No. 6,703,199, which bes fibronectin polypeptide dies).

Antigen binding fragments can be incorporated into single chain molecules comprising a pair of tandem Fv segments (VH-CH1—VH—CH1) which, together with ‘l 9 mentary light chain polypeptides, form a pair of antigen binding regions (Zapata et al., 1995 Protein Eng. 8(10):1057-1062; and U.S. Pat. No. 5,641,870).

As used herein, the term "affinity" refers to the strength of interaction n dy and antigen at single antigenic sites. Within each antigenic site, the variable region of the dy “arm” interacts through weak non-covalent forces with antigen at numerous sites; the more interactions, the stronger the y. As used herein, the term "high affinity" for an lgG antibody or fragment thereof (e.g., a Fab fragment) refers to an antibody having a KD of 10'8 M or less, 10'9 M or less, or ”IO"10 M, or 10'11 M or less, or ‘lO'12 M or less, or 10‘13 M or less for a target antigen. However, high affinity g can vary for other antibody isotypes. For example, high affinity binding for an lgM isotype refers to an antibody having a KD of 10'7 M or less, or 10'8 M or less.

The term "amino acid" refers to lly occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids. Naturally occurring amino acids are those encoded by the genetic code, as well as those amino acids that are later modified, e.g., hydroxyproline, y-carboxyglutamate, and O-phosphoserine. Amino acid s refer to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an alpha carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, 9.9., homoserine, cine, methionine ide, methionine methyl sulfonium. Such analogs have modified R groups (9.9., norleucine) or d peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid. Amino acid mimetics refers to chemical compounds that have a structure that is different from the general Chemical structure of an amino acid, but that functions in a manner similar to a lly ing amino acid.

The term “binding specificity” as used herein refers to the ability of an individual antibody combining site to react with only one antigenic determinant.

The phrase “specifically (or selectively) blnds” to an antibody (e.g., a Factor P- binding antibody) refers to a binding reaction that is determinative of the presence of a cognate antigen (e.g., a human Factor P or cynomolgus Factor P) in a heterogeneous population of proteins and other biologics. The phrases “an antibody recognizing an antigen” and “an antibody specific for an antigen” are used interchangeably herein with the term “an antibody which binds specifically to an antigen”.

The term "conditions or disorders associated with macular degeneration" refers to any of a number of conditions in which the retinal macula rates or becomes ZOIZ/057394 dysfunctional, e.g., as a consequence of decreased growth of cells of the macula, sed death or rearrangement of the cells of the macula (e.g., RPE cells), loss of normal biological function, or a combination of these events. Macular degeneration s in the loss of ity of the histoarchitecture of the cells and/or extracellular matrix of the normal macula and/or the loss of function of the cells of the macula.

Examples of macular degeneration-related disorder include AMD, North Carolina macular dystrophy, Sorsby's fundus dystrophy, Stargardt‘s disease, pattern dystrophy, Best disease, dominant drusen, and malattia leventinese (radial drusen). The term also encompasses extramacular changes that occur prior to, or ing dysfunction and/or degeneration of the . Thus, the term "macular degeneration—related disorder" also broadly includes any condition which alters or damages the integrity or function of the macula (e.g., damage to the RPE or s membrane). For example, the term encompasses retinal detachment, chorioretinal degenerations, retinal degenerations, photoreceptor degenerations, RPE rations, mucopolysaccharidoses, rod-cone dystrophies, cone-rod dystrophies and cone degenerations.

The term “complement component’3 H , complement proteins" or “complement component proteins” refers to the molecules that are involved in activation of the complement . The classical pathway components include, e.g., Ciq, Cir, 018, C4, CZ, C3, C5, CB, CY, C8, 09, and CSb-Q complex (membrane attack complex: MAC).

The alternative pathway components include, e.g., Factor B, Factor D, Factor H, Factor I and Properdin.

The term “cellular activities regulated by the complement pathway” include cell damage resulting from the CSb-Q attack x, vascular permeability changes, contraction and ion of smooth muscle cells, T cell proliferation, immune adherence, aggregation of dendritic cells, tes, granulocyte and platelet, phagocytosis, migration and activation of neutrophils (PMN) and macrophages.

Further, activation of the complement pathways s in the increase of proinflammatory response contributed by the by-products within the complement pathway. ers associated with activation of the ment pathway include nephritis, asthma, reperfusion injury, hemodialysis, rheumatoid arthritis, systemic lupus, psoriasis, le sclerosis, transplantation, Alzheimer’s disease, aHUS, MPGN II, or any other complement-mediated disease. Disorders associated with macular degeneration include AMD, North Carolina r phy, Sorsby's fundus dystrophy, Stargardt's disease, pattern dystrophy, Best disease, dominant drusen, and malattia leventinese (radial drusen), extramacular changes that occur prior to, or PCT/IBZOlZ/057394 following dysfunction and/or degeneration of the macula, retinal detachment, chorioretinal degenerations, retinal degenerations, photoreceptor degenerations, RPE degenerations, mucopolysaccharidoses, rod-cone dystrophies, cone-rod dystrophies and cone degenerations.

The term "chimeric antibody" is an antibody molecule in which (a) the constant , or a portion f, is altered, replaced or exchanged so that the antigen binding site (variable region) is linked to a constant region of a different or altered class, effector function and/or species, or an entirely different le which confers new properties to the chimeric antibody, e.g., an enzyme, toxin, hormone, growth factor, drug, etc.; or (b) the variable region, or a portion thereof, is altered, replaced or exchanged with a variable region having a different or altered antigen icity. For example, a mouse antibody can be modified by replacing its constant region with the constant region from a human globulin. Due to the replacement with a human constant region, the chimeric antibody can retain its specificity in izing the antigen while having reduced antigenicity in human as compared to the original mouse antibody.

The term “Factor P protein” or “Factor P antigen” or “Factor P” are used interchangeably, and refers to the Factor P protein in different species. For example, human Factor P has the sequence as set out in Table ‘l: SEQ ID NO: 401. Human Factor P can be obtained from Complement Tech, Tyler, TX. Cynomolgus Factor P can be purified from cynomolgus serum (protocol adapted from Nakano etal., (1986) J Immunoi Methods 90:77-83). es of Factor P protein from other species are provided in Table 1, SEQ lD N03: 402, 403, 404 or 405, as well as Factor P protein binding domains and fragments (e.g.: SEQ lD N03: 406, 407 or 408). Factor P is also know in the art as “Properdin”.

The term "conservatively modified variant" applies to both amino acid and nucleic acid sequences. With respect to ular nucleic acid sequences, conservatively modified variants refers to those nucleic acids which encode identical or ially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the c code, a large number of functionally identical nucleic acids encode any given protein. For ce, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without ng the d polypeptide. Such nucleic acid variations are "silent variations," which are one species of conservatively modified ions. Every nucleic acid sequence herein which PCT/IBZOIZ/057394 encodes a polypeptide also describes every possible silent variation of the nucleic acid.

One of skill will recognize that each codon in a nucleic acid (except AUG, which is ordinarily the only codon for methionine, and TGG, which is ordinarily the only codon for tryptophan) can be modified to yield a functionally identical molecule. Accordingly, each silent variation of a nucleic acid that encodes a polypeptide is implicit in each described sequence.

For polypeptide sequences, “conservatively modified variants” include individual substitutions, deletions or additions to a polypeptide sequence which result in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art.

Such conservatively modified variants are in addition to and do not exclude polymorphic variants, pecies homologs, and alleles of the invention. The ing eight groups contain amino acids that are conservative substitutions for one another: 1) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) gine (N), Glutamine (Q); 4) ne (R), Lysine (K); 5) Isoleucine (l), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), ine (T); and 8) Cysteine (C), Methionine (M) (see, e.g., Creighton, Proteins (1984)). In some embodiments, the term "conservative sequence modifications" are used to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody ning the amino acid sequence.

The term “epitope” means a protein determinant capable of specific binding to an antibody. Epitopes usually consist of ally active e groupings of les such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. Conformational and nonconformational es are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents.

The term "human antibody", as used herein, is intended to include antibodies having variable regions in which both the ork and CDR regions are derived from sequences of human origin. Furthermore, if the dy contains a nt region, the constant region also is derived from such human sequences, e.g., human germline sequences, or mutated ns of human germline sequences. The human antibodies of the invention may include amino acid residues not encoded by human sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo).

The term ”human monoclonal antibody" refers to antibodies displaying a single binding specificity which have variable regions in which both the framework and CDR regions are derived from human sequences. in one embodiment, the human monoclonal antibodies are produced by a hybridoma which includes a B cell obtained from a transgenic nonhuman animal, e.g., a transgenic mouse, having a genome comprising a human heavy chain transgene and a light chain transgene fused to an immortalized cell.

A "humanized" antibody is an antibody that retains the vity of a non-human antibody while being less immunogenic in humans. This can be achieved, for ce, by retaining the non—human CDR regions and replacing the remaining parts of the antibody with their human counterparts (i.e., the constant region as well as the framework ns of the variable region). See, e.g., Morrison et al., Proc. Natl. Acad.

Sci. USA, 81:6851—6855, 1984; Morrison and Oi, Adv. lmmunol., 44:65-92, 1988; Verhoeyen et al., e, 239:1534-1536, 1988; Padlan, Molec. lmmun., 28:489-498, 1991; and Padlan, Molec. lmmun., 31 :169-217, 1994. Other examples of human engineering logy include, but are not limited to Xoma technology disclosed in US ,766,886.

The terms “identical" or percent “identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same. Two sequences are "substantially cal" if two sequences have a specified percentage of amino acid es or nucleotides that are the same (i.e., 60% ty, optionally 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% identity over a specified region, or, when not specified, over the entire sequence), when compared and aligned for maximum correspondence over a comparison window, or designated region as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Optionally, the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or more preferably over a region that is 100 to 500 or 1000 or more nucleotides (or 20, 50, 200 or more amino acids) in length.

For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm m parameters are designated.

Default m parameters can be used, or alternative parameters can be designated.

The ce comparison thm then ates the percent ce identities for WO 93762 2012/057394 the test sequences relative to the reference sequence, based on the program parameters.

A “comparison window”, as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more y about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous ons after the two sequences are optimally aligned. Methods of alignment of sequences for ison are well known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith and Waterman (1970) Adv. Appl. Math. 224820, by the homology alignment algorithm of Needleman and Wunsch, J. Mol. Biol. 482443, 1970, by the search for similarity method of Pearson and Lipman, Proc. Natl. Acad. Sci. USA 852444, 1988, by computerized implementations of these algorithms (GAP, BESTFlT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, WI), or by manual alignment and visual inspection (see, 9.9., Brent et al., Current Protocols in Molecular y, John Wiley & Sons, Inc. (Ringbou ed., 2003)).

Two examples of algorithms that are suitable for determining percent sequence identity and sequence rity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al., Nuc. Acids Res. 25:3389-3402, 1977; and ul et al., J.

Mol. Biol. 215:403-410, 1990, respectively. re for performing BLAST analyses is publicly available through the al Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive—valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each ce for as far as the cumulative alignment score can be increased.

Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching es; always > 0) and N (penalty score for mismatching es; always < 0). For amino acid sequences, a g matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either ce is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and PCT/[82012/057394 speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) or 10, M=5, N=-4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a ngth of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and ff, Proc. Natl. Acad. Sci. USA 15, 1989) alignments (B) of 50, expectation (E) of 10, M=5, N=-4, and a ison of both strands.

The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul, Proc. Natl. Acad. Sci. USA 90:5873-5787, 1993). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid ces would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum ility in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most ably less than about 0.001.

The percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Blosci., 4:11-17, 1988) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent ty between two amino acid sequences can be determined using the Needleman and Wunsch (J. Mol, Biol. 48:444-453, 1970) algorithm which has been incorporated into the GAP program in the G06 software package (available on the world wide web at gcg.com), using either a Blossom 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6.

Other than percentage of sequence identity noted above, r indication that two nucleic acid sequences or polypeptides are ntially identical is that the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the antibodies raised against the polypeptide encoded by the second nucleic acid, as described below. Thus, a polypeptide is lly substantially identical to a second polypeptide, for example, where the two es differ only by conservative substitutions. r tion that two nucleic acid sequences are substantially identical is that the two molecules or their complements hybridize to each other under stringent conditions, as described below. Yet another indication that two nucleic acid sequences are substantially identical is that the same primers can be used to amplify the sequence.

WO 93762 PCT/IBZOlZ/057394 The term “inhibit (or inhibits) the alternative complement pathway” refers to the ability of Factor P antibodies to interfere with tion of the alternative complement y. Specifically, “inhibit" refers to a statistically significant decrease (i.e., ) in alternative complement activation as measured by one or more assays as described herein, including MAC ion, hemolytic assay, or 03b deposition assay in a cell or subject ing contact with an actor P dy or fragment f as described herein relative to a control. As used herein, “inhibit (or inhibits) the alternative complement pathway” can also refer to a clinically relevant improvement in visual function or retinal anatomy following ent with an anti—Factor P antibody described herein in a patient diagnosed with age related macular degeneration as described below.

The term "isolated antibody“ refers to an antibody that is substantially free of other antibodies having different antigenic specificities (e.g., an isolated antibody that specifically binds Factor P is substantially free of antibodies that specifically bind antigens other than Factor P). An isolated antibody that specifically binds Factor P may, however, have cross-reactivity to other antigens. Moreover, an ed antibody may be substantially free of other cellular material and/or chemicals.

The term "isotype" refers to the antibody class (e.g., lgM, lgE, lgG such as lgG1 or lgG4) that is provided by the heavy chain constant region genes. lsotype also includes modified versions of one of these classes, where modifications have been made to alter the Fc function, for example, to enhance or reduce effector functions or binding to Fc receptors.

The term "Kassoc" or "Ka", as used herein, is intended to refer to the association rate of a particular antibody-antigen interaction, whereas the term "Kdis" or "Kd," as used herein, is intended to refer to the dissociation rate of a particular antibody—antigen interaction. The term "KD", as used herein, is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e. Kd/Ka) and is sed as a molar concentration (M). KD values for antibodies can be determined using methods well established in the art. Methods for determining the KB of an antibody include measuring surface plasmon resonance using a biosensor system such as a Biacore® system, or ing affinity in solution by solution brium titration (SET).

The terms "monoclonal antibody" or "monoclonal antibody ition" as used herein refer to a ation of antibody molecules of single molecular composition. A monoclonal antibody composition displays a single binding specificity and affinity for a particular epitope.

ZOIZ/057394 The term "nucleic acid" is used herein interchangeably with the term “polynucleotide” and refers to deoxyribonucleotides or cleotides and rs thereof in either single— or double—stranded form. The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non—naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner r to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, peptide—nucleic acids (PNAs).

Unless ise indicated, a ular nucleic acid ce also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and mentary sequences, as well as the sequence explicitly indicated. Specifically, as detailed below, rate codon tutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues r et al., Nucleic Acid Res. 1925081, 1991; Ohtsuka eta/., J. Biol. Chem. 260:2605-2608, 1985; and Rossolini eta/., Mol. Cell. Probes 8:91-98, 1994).

The term "operably " refers to a functional relationship between two or more polynucleotide (e.g., DNA) segments. Typically, the term refers to the functional relationship of a transcriptional regulatory ce to a transcribed sequence. For example, a promoter or enhancer sequence is operably linked to a coding sequence if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system. Generally, promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cis—acting. However, some transcriptional regulatory sequences, such as enhancers, need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance.

As used herein, the term, "optimized" means that a nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in the tion cell or organism, generally a eukaryotic cell, for e, a cell of Pichia, a Chinese Hamster Ovary cell (CHO) or a human cell. The optimized nucleotide sequence is engineered to retain completely or as much as possible the amino acid sequence originally encoded by the starting nucleotide sequence, which is also known as the "parental" sequence. The zed sequences herein have been engineered to have codons that are preferred in mammalian cells. However, optimized expression of these PCT/{82012/057394 sequences in other eukaryotic cells or prokaryotic cells is also envisioned herein. The amino acid sequences encoded by optimized nucleotide sequences are also referred to as optimized.

The terms eptide“ and "protein" are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical mimetic of a corresponding naturally occurring amino acid, as well as to naturally occurring amino acid rs and non- naturally occurring amino acid polymer. Unless ise indicated, a particular polypeptide sequence also implicitly encompasses conservatively modified variants thereof.

The term "recombinant human antibody", as used herein, includes all human antibodies that are prepared, expressed, created or isolated by inant means, such as antibodies isolated from an animal (e.g., a mouse) that is transgenic or transchromosomal for human globulin genes or a hybridoma prepared therefrom, antibodies isolated from a host cell ormed to express the human antibody, e.g., from a transfectoma, antibodies ed from a recombinant, combinatorial human antibody library, and antibodies prepared, expressed, created or isolated by any other means that involve splicing of all or a portion of a human immunoglobulin gene, sequences to other DNA sequences. Such recombinant human antibodies have variable regions in which the ork and CDR regions are derived from human germline immunoglobulin sequences. in certain embodiments, however, such recombinant human dies can be ted to in vitro mutagenesis (or, when an animal transgenic for human lg sequences is used, in vivo c mutagenesis) and thus the amino acid sequences of the VH and VL s of the recombinant antibodies are sequences that, while derived from and related to human germline VH and VL sequences, may not naturally exist within the human antibody germline repertoire in vivo.

The term “recombinant host cell” (or simply “host cell”) refers to a cell into which a recombinant expression vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the y of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein. 2012/057394 The term “subject" includes human and non-human animals. Non-human animals e all vertebrates (e.g.: mammals and non-mammals) such as, non-human primates (e.g.: cynomolgus monkey), sheep, dog, cow, chickens, amphibians, and reptiles. Except when noted, the terms “patient” or “subject” are used herein interchangeably. As used herein, the terms “cyno” or “cynomolgus” refer to the cynomolgus monkey (Macaca fascicularis).

As used herein, the term “treating" or "treatment" of any e or disorder (i.e., AMD) refers in one embodiment, to ameliorating the disease or disorder (i.e., g or arresting or reducing the development of the disease or at least one of the clinical symptoms thereof). In another embodiment "treating" or "treatment" refers to alleviating or ameliorating at least one physical parameter including those which may not be discernible by the patient. In yet r embodiment, "treating" or "treatment" refers to modulating the disease or disorder, either physically, (e.g., stabilization of a nible symptom), physiologically, (e.g., stabilization of a physical parameter), or both. in yet another embodiment, "treating" or "treatment" refers to preventing or delaying the onset or development or ssion of the disease or disorder. “Prevention” as it relates to AMD means any action that prevents or slows a ing in visual function, retinal anatomy, and/or an AMD disease parameter, as described below, in a patient at rist for said worsening. More specifically, “treatment” of AMD means any action that s in the improvement or preservation of visual function and/or reginal anatomy. Methods for assessing treatment and/or prevention of disease are known in the art and bed hereinbelow.

The term “vector” is intended to refer to a polynucleotide molecule capable of transporting another polynucleotide to which it has been linked. One type of vector is a “plasmid”, which refers to a ar double stranded DNA loop into which additional DNA segments may be ligated. Another type of vector is a viral vector, such as an adeno- ated viral vector (AAV, or AAV2), wherein additional DNA segments may be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian s). Other vectors (e.g., non-episomal mammalian vectors) can be ated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are e of ing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “recombinant expression vectors” (or simply, ssion vectors”). In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. in the present PCT/IBZOlZ/057394 specification, “plasmid” and “vector” may be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 Depicts the Factor P binding site. Firgure 1A illustrates the relative position TSR5 domain of Factor P and the TSR5 sequence fragments: A, B, C and D. Figure 1B shows the human TSR5 sequence aligned with the mouse sequence. Brackets te the sequence fragments of TSR5. Figure 10 illustrates the antibodies bind to region B of TSR5.

Figure 2 shows the results of a hemolytic assay trating inhibition of the alternative ment y in 20% human serum.

Figure 3 shows an isobologram generated using the data from the tic assay depicted in Figure 2.

Figure 4 shows the % inhibition of macrophage infiltrates in a mouse poly-IC model, comparing the inhibition of anti-FactorP and anti-CS antibodies singly and in combination.

Figure 5 shows an isobologram generated using the data from the poly—IC results depicted in Figure 4.

DETAILED DESCRIPTION The present invention is based, in part, on the ery of antibody molecules that specifically bind to both human and cynomolgus Factor P. The invention s to both full IgG format antibodies as well as n binding fragments thereof, such as Fab fragments (e.g., see antibodies NVSQSS—S, NVSQGZ-S, NVS804 and NV8807 ).

Accordingly, the present ion provides antibodies that specifically bind to Factor P (e.g., human Factor P, cynomolgus Factor P, rat Factor P, rabbit Factor P), pharmaceutical compositions, production methods, and methods of use of such dies and compositions.

PCT/IBZOlZ/057394 Factor P Antibodies & Antigen Binding Fragments The present ion provides antibodies that specifically bind to Factor P. In some embodiments, the present invention provides antibodies that specifically bind to human, cynomolgus, rat and/or rabbit Factor P. Antibodies of the ion e, but are not limited to, the human monoclonal antibodies and Fabs, isolated as described in the es.

The present invention provides antibodies that specifically bind a Factor P n (e.g., human and/or cynmolgus Factor P), wherein the antibodies comprise a VH domain having an amino acid sequence of SEQ lD NO: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 or 273. The t invention also provides antibodies that specifically bind to a Factor P protein, wherein the antibodies comprise a VH CDR having an amino acid sequence of any one of the VH CDRs listed in Table 1, infra. In particular, the invention provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), wherein the antibodies comprise (or alternatively, consist of) one, two, three, or more VH CDRs having an amino acid sequence of any of the VH CDRs listed in Table 1, infra.

The t invention provides antibodies that specifically bind to a Factor P protein, said antibodies comprising a VL domain having an amino acid sequence of SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274. The present invention also provides antibodies that cally bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), said dies comprising a VL CDR having an amino acid sequence of any one of the VL CDRs listed in Table 1, infra. In ular, the invention provides antibodies that specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P), said antibodies comprising (or alternatively, consisting of) one, two, three or more VL CDRs having an amino acid sequence of any of the VL CDRs listed in Table 1, infra.

Other antibodies of the invention include amino acids that have been mutated, yet have at least 60, 70, 80, 85, 90 or 95 percent identity in the CDR regions with the CDR regions depicted in the sequences described in Table 1. In some embodiments, it includes mutant amino acid sequences wherein no more than 1, 2, 3, 4 or 5 amino acids have been mutated in the CDR regions when compared with the CDR regions ed in the sequence described in Table 1.

The present invention also provides nucleic acid sequences that encode VH, VL, the full length heavy chain, and the full length light chain of the antibodies that PCT/lBZOlZ/057394 specifically bind to a Factor P protein (e.g., human and/or cynomolgus Factor P). Such nucleic acid sequences can be optimized for expression in mammalian cells (for example, Table 1 shows the optimized nucleic acid sequences for the heavy chain and htofanfibodbs<fitheinvenfionl Table 1 Examples of Factor P dies, Fabs and Factor P Proteins AMINO ACID SEQUENCE IDENTIFIER (SEQ.ID.NO:)AND SEQUENCE SEQUENCE OR POLYNUCLEOTIDE (PN) NV8962 CDRHl 1/281 SYAIS (Kabat)/ GGTFNSY (Chothia) CDRHZ 2/282 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) “283 HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) “284 SKYVD (Kabat)/ DNLGSKY (Chothia) “285 SDNNRPS (Kabat)/ SDN (Chothia) “286 QTYTSGNNYL (Kabat)/ YTSGNNYL (Chothia) VH 7 SGAEVKKPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 9 SGAEVKKPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 10 PPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG KADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 11 SEQ.ID.NO:7 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG W0 2013(093762 —GTGACCGTGAGCTCA PN ENCODING 12 SEQ.ID.NO:8 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN NG l3 SEQ.ID.NO:9 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC AGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING l4 SEQ.ID.NO:10 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG AGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC GGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA NV8963 CDRHl 15/287 SYAIS (Kabat)/ GGTFSSY (Chothia) CDRH2 16/288 PINPYYGDAIYAQKFQG (Kabat)/ NPYYGD (Chothia) CDRH3 17/289 YYSDYMDY (Kabat)/ YYSDYMDY (Chothia) CDRLl 18/290 IGAGYDVH (Kabat)/ SSSNIGAGYD (Chothia) CDRL2 19/291 DNSHRPS (Kabat)/ DNS (Chothia) CDRL3 20/292 ASYDESAHS (Kabat)/ YDESAHS (Chothia) VH 21 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GPINPYYGDAIYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC WO 93762 —ARYYSDYMDYWGQGTLVTVSS QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKL LIHDNSHRPSGVPDRFSGSKSGTSASLAITGLQSEDEADYYCASYDES GGTKLTVL , HEAVY CHAIN 23 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GPINPYYGDAIYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARYYSDYMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 24 QSVLTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKL LIHDNSHRPSGVPDRFSGSKSGTSASLAITGLQSEDEADYYCASYDES AHSVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFY PGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSH RSYSCQVTHEGSTVEKTVAPTECS PN ENCODING 25 SEQ.ID.NO:21 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTTAGCAGCTAC AGCTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCCCCATCAACCCCTACTACGGCGACGCCATCTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCCGGTACTACAGCGACTACATGGACTACTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 26 SEQ.ID.NO:22 CAGTCAGTGCTGACCCAGCCTCCCTCTGTGTCTGGCGCCCCTGGCCAG AGAGTGACCATCAGCTGCACCGGCTCCAGCAGCAACATCGGAGCTGGA TACGACGTGCACTGGTATCAGCAGCTGCCCGGCACAGCCCCTAAGCTG CTGATCCACGACAACAGCCACAGACCCAGCGGCGTGCCCGATAGATTC AGCGGCAGCAAGAGCGGCACCAGCGCCAGCCTGGCCATCACCGGCCTG CAGAGCGAGGACGAGGCCGACTACTACTGCGCCAGCTACGACGAGAGC GCCCACAGCGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 27 SEQ.ID.NO:23 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTTAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCCCCATCAACCCCTACTACGGCGACGCCATCTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCCGGTACTACAGCGACTACATGGACTACTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA CTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 28 SEQ.ID.NO:24 CAGTCAGTGCTGACCCAGCCTCCCTCTGTGTCTGGCGCCCCTGGCCAG AGAGTGACCATCAGCTGCACCGGCTCCAGCAGCAACATCGGAGCTGGA TACGACGTGCACTGGTATCAGCAGCTGCCCGGCACAGCCCCTAAGCTG CTGATCCACGACAACAGCCACAGACCCAGCGGCGTGCCCGATAGATTC AGCGGCAGCAAGAGCGGCACCAGCGCCAGCCTGGCCATCACCGGCCTG CAGAGCGAGGACGAGGCCGACTACTACTGCGCCAGCTACGACGAGAGC PCT/IBZOIZ/057394 GCCCACAGCGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAG CCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTAC CCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAG GCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTAC GCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC AGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAG ACAGTGGCCCCTACAGAATGTTCA CDRHl 29/293 SHYMH (Kabat)/ GYTFTSH (Chothia) CDRH2 30/294 KINADLGDTNYAQKFQG )/ NADLGD (Chothia) CDRH3 31/295 DGIEHGGHYYWGYLFDI (Kabat)/ DGIEHGGHYYWGYLFDI (Chothia) 32/296 SGDSIREYYVH (Kabat)/ DSIREYY (Chothia) CDRLZ 33/297 DDTNRPS (Kabat)/ DDT (Chothia) CDRL3 34/298 AAWDFSPAI )/ WDFSPAI (Chothia) EVQLVQSGAEVKKPGASVKVSCKASGYTFTSHYMHWVRQAPGQGLEWM GKINADLGDTNYAQKFQGRVTMTRDTSISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDSIREYYVHWYQQKPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCAAWDFSPAI VFGGGTKLTVL HEAVY CHAIN 37 EVQLVQSGAEVKKPGASVKVSCKASGYTFTSHYMHWVRQAPGQGLEWM GKINADLGDTNYAQKFQGRVTMTRDTSISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSSASTKGPSVFPLAPSSKST SGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 38 SYELTQPPSVSVAPGQTARISCSGDSIREYYVHWYQQKPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCAAWDFSPAI VFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGA VTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSY EGSTVEKTVAPTECS PN ENCODING 39 SEQ.ID.NO:35 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAAGATCAACGCCGACCTGGGCGACACCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCA PN ENCODING 4O SEQ.ID.NO:36 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG AGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC W0 20131‘093762 ACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 41 SEQ.ID.NO:37 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAAGATCAACGCCGACCTGGGCGACACCAACTACGCCCAGAAATTC AGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCAGCATCC ACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACC TCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTG CACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGC AGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATC GTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGT PN ENCODING 42 SEQ.ID.NO:38 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAGGCT GCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCC AACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCC GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG ACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC AGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTAC AGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCC GAATGTTCA NV3966 CDRHl 43/299 NYWIG (Kabat)/ GYSFTNY (Chothia) CDRH2 44/300 RIDPGESLTNYAPSFQG (Kabat)/ DPGESL (Chothia) CDRH3 45/301 TGVADVDMPFAH (Kabat)/ TGVADVDMPFAH (Chothia) CDRLl 46/302 SGDNLGSYYVN (Kabat)/ DNLGSYY (Chothia) CDRL2 47/303 GDSERPS (Kabat)/ GDS (Chothia) CDRL3 48/304 GSWDITSF (Kabat)/ WDITSF ia) EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYWIGWVRQMPGKGLEWM GRIDPGESLTNYAPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYC ARTGVADVDMPFAHWGQGTLVTVSS SYVLTQPPSVSVAPGKTARISCSGDNLGSYYVNWYQQKPGQAPVLVIY ZOlZ/057394 GDSERPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCGSWDITSFV FGGGTKLTVL HEAVY CHAIN 51 EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYWIGWVRQMPGKGLEWM GRIDPGESLTNYAPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYC ARTGVADVDMPFAHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTA ALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTV PSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 52 PPSVSVAPGKTARISCSGDNLGSYYVNWYQQKPGQAPVLVIY SGIPERFSGSNSGNTATLTISRAQAGDEADYYCGSWDITSFV FGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAV TVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYS CQVTHEGSTVEKTVAPTECS PN ENCODING 53 SEQ.ID.NO:49 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAGCCTGGCGAG AGCCTGAAGATCAGCTGCAAGGGCAGCGGCTACAGCTTCACCAACTAC TGGATCGGCTGGGTGCGCCAGATGCCTGGCAAGGGCCTGGAATGGATG GGCAGAATCGACCCCGGCGAGTCCCTGACCAACTACGCCCCCAGCTTC CAGGGCCAGGTGACAATCAGCGCCGACAAGAGCATCAGCACCGCCTAT CTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC GCCAGAACCGGCGTGGCCGACGTGGACATGCCTTTTGCCCACTGGGGC CAGGGCACCCTGGTGACCGTGAGCTCA PN NG 54 SEQ.ID.NO:50 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCTACTACGTG AACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC GGCGACAGCGAGAGGCCTAGCGGCATCCCCGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGGCTCCTGGGACATCACCAGCTTCGTG TTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 55 SEQ.ID.NO:51 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAGCCTGGCGAG AGCCTGAAGATCAGCTGCAAGGGCAGCGGCTACAGCTTCACCAACTAC TGGATCGGCTGGGTGCGCCAGATGCCTGGCAAGGGCCTGGAATGGATG GGCAGAATCGACCCCGGCGAGTCCCTGACCAACTACGCCCCCAGCTTC CAGGGCCAGGTGACAATCAGCGCCGACAAGAGCATCAGCACCGCCTAT CTGCAGTGGAGCAGCCTGAAGGCCAGCGACACCGCCATGTACTACTGC ACCGGCGTGGCCGACGTGGACATGCCTTTTGCCCACTGGGGC CAGGGCACCCTGGTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCG GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCG GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTG TCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCT GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTG CCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCAC AAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 56 SEQ.ID.NO:52 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCTACTACGTG AACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC GGCGACAGCGAGAGGCCTAGCGGCATCCCCGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGGCTCCTGGGACATCACCAGCTTCGTG TTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAGGCTGCC CCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAAC AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTG ACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAG ACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGC TATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGC TGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCT ACAGAATGTTCA NV8965 CDRHl 57/305 SYAIS (Kabat)/ GGTFNSY (Chothia) CDRH2 58/306 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) CDRH3 59/307 HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) CDRLl 60/308 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) CDRL2 61/309 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 62/310 ATYDSSPRTE (Kabat)/ YDSSPRTE (Chothia) V 63 EVQLVQSGAEVKKPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVL HEAVY CHAIN 65 EVQLVQSGAEVKKPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 66 PPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG KADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 67 SEQ.ID.NO:63 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC CTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN NG 68 SEQ.ID.NO:64 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG AGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG TATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG PCT/IBZOIZ/057394 _—GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 69 SEQ.ID.NO:65 CAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC CTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 7O SEQ.ID.NO:66 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC TTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA ACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC TATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA NVS967 c DRHl 71/3:1 SHYMH (Kabat)/ GYTFTSH (Chothia) CDRH2 72/3;2 NINPVDGGTEYAQKFQG (Kabat)/ NPVDGG (Chothia) CDRH3 73/3L3 DGIEHGGHYYWGYLFDI (Kabat)/ DGIEHGGHYYWGYLFDI (Chothia) CDRLl 74/3:4 SGDSIREYYVH (Kabat)/ DSIREYY (Chothia) CDRLZ 75/315 DDTNRPS (Kabat)/ DDT (Chothia) CDRL3 76/316 AAWDFSPAI )/ WDFSPAI (Chothia) Iiillllllllllll 77 EVQLVQSGAEVKKPGASVKVSCKASGYTFTSHYMHWVRQAPGQGLEWM GNINPVDGGTEYAQKFQGRVTMTRDTSISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSS vL 78 SYVLTQPPSVSVAPGKTARISCSGDSlREYYVHWYQQKPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCAAWDFSPAI VFGGGTKLTVL W0 2013I093762 ZOlZ/057394 SGAEVKKPGASVKVSCKASGYTFTSHYMHWVRQAPGQGLEWM DGGTEYAQKFQGRVTMTRDTSISTAYMELSSLRSEDTAVYYC ARDGIEHGGHYYWGYLFDIWGQGTLVTVSSASTKGPSVFPLAPSSKST SGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 8O SYVLTQPPSVSVAPGKTARISCSGDSIREYYVHWYQQKPGQAPVLVIG DDTNRPSGIPERFSGSNSGNTATLTISRAQAGDEADYYCAAWDFSPAI VFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGA VTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSY SCQVTHEGSTVEKTVAPTECS PN ENCODING 81 SEQ.ID.NO:77 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAACATCAACCCCGTGGACGGCGGCACCGAGTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCA PN ENCODING 82 SEQ.ID.NO:78 TCTTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN NG 83 SEQ.ID.NO:79 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCTGGCGCC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCTACACCTTCACCAGCCAC TACATGCACTGGGTGCGCCAGGCTCCAGGACAGGGCCTGGAATGGATG GGCAACATCAACCCCGTGGACGGCGGCACCGAGTACGCCCAGAAATTC CAGGGCAGAGTGACCATGACCCGGGACACCAGCATCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGC GCCAGGGACGGCATCGAGCACGGCGGCCACTACTACTGGGGCTACCTG TTCGACATCTGGGGCCAGGGCACCCTGGTGACCGTGAGCTCAGCCTCC ACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACC TCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTG CACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGC AGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATC TGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGT PN NG 84 SEQ.ID.NO:8O TCTTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAGCATCCGGGAGTACTACGTG CACTGGTATCAGCAGAAGCCCGGCCAGGCTCCTGTGCTGGTGATCGGC GACGACACCAACAGACCCAGCGGCATCCCCGAGAGATTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCTCTAGAGCCCAGGCCGGC GACGAGGCCGATTACTACTGCGCCGCCTGGGACTTCAGCCCTGCCATC GTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAGGCT TCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCC AACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCC GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG GAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC PCT/132012/057394 AGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTAC AGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCC GAATGTTCA 85/317 SYAIS (Kabat)/ GGTFSSY (Chothia) CDRH2 86/318 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) CDRH3 87/319 HGGYSYFDS (Kabat)/ HGGYYFDS (Chothia) CDRLl 88/320 SKYVD (Kabat)/ DNLGSKY ia) CDRL2 89/321 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 90/322 QTYTSGNNYL (Kabat)/ YTSGNNYL (Chothia) EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 93 SGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 94 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 95 SEQ.ID.NO:91 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC AGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGAGCTCA PN ENCODING 96 SEQ.ID.NO:92 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 97 SEQ.ID.NO:93 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC PCT/182012/057394 AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC AGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC CTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 98 SEQ. ID.NO:94 GAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA CDRHl 99/323 SYAIS (Kabat)/ GGTFSSY (Chothia) CDRH2 100/324 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) CDRH3 101/325 HGGYIFDS (Kabat)/ HGGYIFDS (Chothia) CDRLl 102/326 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) CDRLZ 103/327 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 104/328 QTYTSGNNYL (Kabat)/ YTSGNNYL (Chothia) VH 105 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 107 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQS SGLYSLSSWTVPS S S LGTQTY I CNVNHKPSNTKVDKRVEPKS C W0 2013.1093762 LIGHT CHAIN 108 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 109 SEQ.ID.NO:105 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 110 SEQ.ID.NO:106 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC GGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 111 SEQ.ID.NO:107 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC CTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC ACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 112 SEQ.ID.NO:108 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC PCT/IBZOIZ/057394 AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA steo6 — CDRHl 113/329 SYAIS (Kabat) / GGTFSSY (Chothia) CDRH2 114/330 RIIPIFGTANYAQKFQG (Rabat) / IPIFGT (Chothia) CDRH3 115/331 HGGYVFDS (Kabat) / HGGYVFDS ia) CDRLl 2 SKYVD (Kabat) / DNLGSKY (Chothia) CDRL2 117/333 SDNNRPS (Kabat) / SDN (Chothia) CDRL3 118/334 QTYTSGNNYL (Kabat) / YTSGNNYL (Chothia) EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 121 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 122 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG KADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 123 SEQ.ID.NO:119 CAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG ATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 124 SEQ.ID.NO:12O AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG TATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 125 SEQ.ID.NO:121 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC W0 20131093762 AAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC AGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 126 SEQ.ID.NO:122 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC TTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA ””3” SYAIS (Kabat)/ GGTFSSY (Chothia) 128”” RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) 129/337 HGGYVFDS (Kabat)/ HGGYIFDS (Chothia) 130/338 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) SDNNRPS (Kabat)/ SDN (Chothia) ”234‘” PRTE (Kabat)/ YDSSPRTE (Chothia) VH 133 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC IFDSWGQGTLVTVSS lii|||||||||||||||| 134SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIYSDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRTEVFGGGTKLTVL HEAVY CHAIN 135 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYIFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC 2012/057394 LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS ICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 136 SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING I37 SEQ.ID.NO:133 CAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 138 SEQ.ID.NO:134 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 139 SEQ.ID.NO:135 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACATTTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 140 SEQ.ID.NO:136 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG CCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA SYAIS (Kabat)/ GGTFSSY (Chothia) GTANYAQKFQG (Kabat)/ IPIFGT (Chothia) DS )/ HGGYYFDS (Chothia) SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) C W SDNNRPS (Kabat)/ SDN (Chothia) PRTE (Kabat)/ YDSSPRTE (Chothia) -EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMVH 147 GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSS IiiIIIIIIIIIIIII 148SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIYSDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRTEVFGGGTKLTVL HEAVY CHAIN 149 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYYFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 150 SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 151 SEQ.ID.NO:147 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 152 SEQ.ID.NO:148 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 153 .NO:149 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC CTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACTACTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACcCTCCTcCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 154 SEQ.ID.NO:150 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG AGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCc AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA CDRHl 155/347 SYAIS (Kabat)/ GGTFSSY ia) CDRHZ 156/348 GTANYAQKFQG (Kabat)/ IPIFGT (Chothia) CDRH3 157/349 HGGYVFDS (Kabat)/ HGGYVFDS (Chothia) CDRLl :58/350 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) CDRLZ :59/351 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 160/352 ATYDSSPRTE (Kabat)/ TE (Chothia) VH I61 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSS SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVL HEAVY CHAIN 163 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYVFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 164 SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG 2012/057394 AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN NG 165 SEQ.ID.NO:161 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 166 SEQ.ID.NO:162 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC TTCGGAGGCGGAACAAAGTTAACCGTCCTA PN NG 167 SEQ.ID.NO:163 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACGTCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 168 SEQ.ID.NO:164 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG CCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA NVS962—S SYAIS (Kabat)/ GGTFSSY (Chothia) RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) W0 20131093762 ”“5” SKYVD (Kabat)/ DNLGSKY (Chothia) “33” SDNNRPS (Kabat)/ SDN (Chothia) ””5” QTYTSGNNYL )/ YTSGNNYL (Chothia) -EVOLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMVH 175 GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS IiiIIIIIIIIIIIIII 176SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIYSDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNYLVFGGGTKLTVL HEAVY CHAIN 177 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 178 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 179 SEQ.ID.NO:175 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 180 SEQ.ID.NO:176 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC GGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 181 SEQ.ID.NO:177 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC ACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 182 .NO:178 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG TATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA CDRHl 183/359 SYAIS (Kabat)/ GGTFQSY (Chothia) CDRHZ 184/360 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) CDRH3 185/361 HGGYSFDS (Kabat)/ HGGYSFDS ia) CDRLl 186/362 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) CDRL2 3 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 188/364 QTYTSGNNYL (Kabat)/ YTSGNNYL (Chothia) VH 189 EVQLVQSGAEVKKPGSSVKVSCKASGGTFQSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 191 EVQLVQSGAEVKKPGSSVKVSCKASGGTFQSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 192 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING PCT/IBZOlZ/057394 SEQ.ID.NO:189 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 194 SEQ.ID.NO:19O GAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG TATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 195 SEQ.ID.NO:191 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC CTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 196 SEQ.ID.NO:192 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA NV8962-S31A SYAIS (Kabat)/ GGTE‘NAY (Chothia) RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) SKYVD (Kabat)/ DNLGSKY (Chothia) 201/369 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 0 NNYL (Kabat)/ YTSGNNYL (Chothia) liillllllllllll 203EVQLVQSGAEVKKPGSSVKVSCKASGGTFNAYAISWVRQAPGQGLEWMGRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARHGGYSFDSWGQGTLVTVSS Iiilllllllllllll 204SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIYSDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNYLVFGGGTKLTVL HEAVY CHAIN 205 EVQLVQSGAEVKKPGSSVKVSCKASGGTFNAYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 206 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 207 SEQ.ID.NO:203 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACGCCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC CTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 208 SEQ.ID.NO:204 AGCTACGAGCTGACTCAGCCCCCITCTGTGTCTGTGGCCCCTGGCCAG AGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 209 SEQ.ID.NO:205 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAACGCCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC AGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PCT/IBZOlZ/057394 PN ENCODING 210 SEQ. ID.NO:206 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA CJRHl 211/371 SYAIS (Kabat)/ GGTFGSY (Chothia) O 3RH2 212/372 RZIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) o 3RH3 3 HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) r) DRLl 214/374 SGDNLGSKYVD )/ DNLGSKY (Chothia) O DRLZ 215/375 SDNNRPS (Kabat)/ SDN (Chothia) 0 3RL3 2:6/376 QTYTSGNNYL (Kabat)/ YTSGNNYL (Chothia) <1:1: 2:7 EVQLVQSGAEVKKPGSSVKVSCKASGGTFGSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS <t‘i 218 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 219 SGAEVKKPGSSVKVSCKASGGTFGSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 220 PPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN NG 221 SEQ. ID.NO:217 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCGGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC W0 20131093762 ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 222 SEQ.ID.NO:218 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 223 SEQ.ID.NO:219 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCGGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC CTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA CTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 224 SEQ.ID.NO:220 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG TATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA CDRHI 225/377 SYAIS (Kabat)/ GGTFTSY (Chothia) CDRHZ 8 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) CDRH3 227/379 HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) CDRLl 228/380 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) CDRL2 229/381 SDNNRPS (Kabat)/ SDN (Chothia) CDRL3 230/382 NNYL (Kabat))/ YTSGNNYL (Chothia) PCT/IBZOIZ/057394 EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVL HEAVY CHAIN 233 EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 234 SYELTQPPSVSVAPGQTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAEDEADYYCQTYTSGNNY LVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 235 SEQ.ID.NO:23I CAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 236 SEQ.ID.NO:232 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 237 SEQ.ID.NO:233 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC CTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 238 SEQ.ID.NO:234 AGCTACGAGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCCAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG TATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC PCT/[82012/057394 AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAG GACGAGGCCGACTACTACTGCCAGACCTACACCAGCGGCAACAACTAC CTGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG CCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA NVS965-T 23938” SYAIS (Kabat)/ GGTFTSY (Chothia) GTANYAQKFQG (Kabat)/ IPIFGT (Chothia) HGGYSEDS (Kabat)/ HGGYSFDS (Chothia) SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) SDNNRPS (Kabat)/ SDN (Chothia) “4388/ ATYDSSPRTE (Kabat)/ YDSSPRTE (Chothia) IIIIIIIIIIH 245 EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS llilllllllllllllll 246SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIYSDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRTEVFGGGTKLTVL HEAVY CHAIN 247 EVQLVQSGAEVKKPGSSVKVSCKASGGTFTSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC SFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 248 SYVLTQPPSVSVAPGKTAR:SCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 249 .NO:245 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 250 SEQ.ID.NO:246 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG W0 20132‘093762 GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING 251 SEQ.ID.NO:247 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCACCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC GTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 252 SEQ.ID.NO:248 GTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA GCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA NVS965-Q SYAIS (Kabat)/ GGTFQSY (Chothia) RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT ia) HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) SGDNLGSKYVD (Kabat)/ Y (Chothia) SDNNRPS (Kabat)/ SDN (Chothia) ATYDSSPRTE (Kabat)/ YDSSPRTE (Chothia) Iii|||||||||||||| LVQSGAEVKKPGSSVKVSCKASGGTFQSYAISWVRQAPGQGLEWMGRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARHGGYSFDSWGQGTLVTVSS Iiillllllllllllllll 260SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIYSDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT W0 20132093762 —EVFGGGTKLTVL HEAVY CHAIN 261 EVQLVQSGAEVKKPGSSVKVSCKASGGTFQSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC LVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 262 SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG KADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 263 SEQ.ID.NO:259 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC AGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 264 SEQ.ID.NO:260 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTA PN NG 265 .NO:26I CAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCCAAAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC TTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 266 SEQ.ID.NO:262 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACccTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA CDRHl 267/395 SYAIS (Kabat)/ GGTFSSY ia) DRHZ 268/396 RIIPIFGTANYAQKFQG (Kabat)/ IPIFGT (Chothia) DRH3 269/397 HGGYSFDS (Kabat)/ HGGYSFDS (Chothia) 3RL1 270/398 SGDNLGSKYVD (Kabat)/ DNLGSKY (Chothia) O 3RL2 271/399 SDNNRPS (Kabat)/ SDN (Chothia) O 3RL3 272/400 ATYDSSPRTE (Kabat)/ YDSSPRTE (Chothia) L 273 SGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM GRIIPIFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSS L“ 274 SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVL HEAVY CHAIN 275 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWM FGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYC ARHGGYSFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGC PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSS LGTQTYICNVNHKPSNTKVDKRVEPKSC LIGHT CHAIN 276 SYVLTQPPSVSVAPGKTARISCSGDNLGSKYVDWYQQKPGQAPVLVIY SDNNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYDSSPRT EVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPG AVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRS YSCQVTHEGSTVEKTVAPTECS PN ENCODING 277 SEQ . ID.NO:273 GAGGTGCAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC AGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT GCCCGGCACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCA PN ENCODING 278 SEQ. ID.NO:274 AGCTACGTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC TTCGGAGGCGGAACAAAGTTAACCGTCCTA PN ENCODING WO 2013093762 PCT/IBZOIZ/057394 SEQ.ID.NO:275 CAGCTGGTGCAGAGCGGAGCCGAAGTGAAGAAACCCGGCAGC AGCGTGAAGGTGTCCTGCAAGGCCAGCGGCGGCACCTTCAGCAGCTAC GCCATCAGCTGGGTGCGCCAGGCTCCTGGACAGGGCCTGGAATGGATG GGCCGGATCATCCCCATCTTCGGCACCGCCAACTACGCCCAGAAATTC CAGGGCAGAGTGACCATCACCGCCGACGAGAGCACCAGCACCGCCTAC ATGGAACTGAGCAGCCTGAGAAGCGAGGACACCGCCGTGTACTACTGT CACGGCGGCTACAGCTTCGATAGCTGGGGCCAGGGCACCCTG GTGACCGTGAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGC CTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCA GGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCC TCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC ACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC ACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGT PN ENCODING 280 SEQ.ID.NO:276 GTGCTGACTCAGCCCCCTTCTGTGTCTGTGGCCCCTGGCAAG ACCGCCAGAATCAGCTGCAGCGGCGACAACCTGGGCAGCAAATACGTG GACTGGTATCAGCAGAAGCCCGGCCAGGCTCCCGTGCTGGTGATCTAC AGCGACAACAACCGGCCCAGCGGCATCCCTGAGCGGTTCAGCGGCAGC AACAGCGGCAATACCGCCACCCTGACCATCAGCGGCACCCAGGCCATG GACGAGGCCGACTACTACTGCGCCACCTACGACAGCAGCCCCAGAACC GAGGTGTTCGGAGGCGGAACAAAGTTAACCGTCCTAGGTCAGCCCAAG GCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAA AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGA GCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGA GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGC TACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTG GCCCCTACAGAATGTTCA Human Factor P 40l NP_OOll38724.l PVLCFTQYEESSGKCKGLLGGGVSVEDCCLNTAFAYQKRSGGLCQPCR SPRWSLWSTWAPCSVTCSEGSQLRYRRCVGWNGQCSGKVAPGTLEWQL QACEDQQCCPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAPKCGG HCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETR SRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSP CPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDG EWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQ QDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLP KYPPTVSMVEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPA CKDPEEEEL Chimpanzee 402 Factor P MITEGAQAPCLLLPPLLLLLTLPATGSDPVLCFTQYEESSGKCKGLLG XP_001136665.1 GGVSVKDCCLNTAYAYQERNGGLCQPCRSPRWSLWSTWAPCSVTCSEG SQLRYRRCVGWNGQCSERVALGTLEWQLQACEDKQCCPEMGGWSDWGP WEPCSVTCSKGMRTRRRACNHPAPKCGGHCPGEAQESEACDTQQVCPT WGPWSPCSGSCHGGPHEPKETRSRTCSAPEPSQKPPGKPCPG PAYE PQHGGPSCAGDATRTHICNTAAPCPVDGEWDLWGQWSTCVRRNMKSIS CEEIPGQQSRWRTCKGRKFDGHRCTGQQQDIRHCYSIQHCPLKGSWSE WSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFW GRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEKL Rat Factor P 403 NP_001100227.1 MPVGMQAPQWLLLLLLILPTTGSDPVLCFTQYEEPSGRCKGLLGRDIR VEDCCLNTAYAFQEHDGGLCQSCRSPQWSAWSSWGPCSVTCSEGSQLR HRRCVGRGGQCSEKAAPGTLEWQLQACEDQLCCPEMGGWSEWGPWGPC SVTCSKGTQTRQRLCDNPAPKCGGHCPGEAQQSQACDTQKICPTHGAW ASWGPWSACSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGTAYE HRGCSGLPPCPVAGGWGPWGPSSPCPVTCGLGQTLERRTCDHPVPRHG GPFCAGDATRKHVCNTAMPCPVNGEWEAWGKWSHCSRVRMKSISCDEI PGQQSRSRSCGGRKFDGQPCTGKLQDIRHCYDIHNCVLKGSWSQWSTW GLCTPPCGPNPTRVRQRLCTPLLPKYSPTVSMVEGQGEKNVTFWGIPR QGQKLVVEEKRPCLHVPSCRDPEEKKP Rabbit Factor P 404 XP_002719931.1 PPLPLLLLPLLLTLPATGADPVVCFTEYDEPSGKCKGLLGGG VSVEHCCLNAAYAFQEPGSGLCHACRSPLWSPWSAWAPCSVTCSEGSQ LRHRRCVGQGGPCSEKAAPGTLQWQLQACEDQPCCPEIGGWSDWGPWR PCSVTCSKGTKTRQRACDRPAPKCGGRCPGEAQESEACDTKQVCPTHG LWAAWGPWSPCSGSCHGGPQVPKETRSRTCSAPEPSKQPPGKPCSGPA YEEQSCAGLPPCPVAGGWGPWGPVSSCSVTCGLGKTLEKRTCDHPVPQ HGGPFCTGDATRTHICNTAVPCPVNGEWEAWGEWSECSRPGRKSISCE EVPGQQRRTRVCKGRKFDGQRCAGEYQDIRHCYNIQRCRLKGSWLEWS SWGLCTPPCGPSPTRTRQRLCTALLPKFPPTISLVEGQGEKNVTFWGK PWPQCEQLQGQKLVVEEKRPCLHVPACKDPEEKP Mouse Factor P 405 NP_O32849.2 MPAEMQAPQWLLLLLVILPATGSDPVLCFTQYEESSGRCKGLLGRDIR VEDCCLNAAYAFQEHDGGLCQACRSPQWSAWSLWGPCSVTCSEGSQLR HRRCVGRGGQCSENVAPGTLEWQLQACEDQPCCPEMGGWSEWGPWGPC SVTCSKGTQIRQRVCDNPAPKCGGHCPGEAQQSQACDTQKTCPTHGAW ASWGPWSPCSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGPAYE HKACSGLPPCPVAGGWGPWSPLSPCSVTCGLGQTLEQRTCDHPAPRHG GPFCAGDATRNQMCNKAVPCPVNGEWEAWGKWSDCSRLRMSINCEGTP RSCGGRKFNGKPCAGKLQDIRHCYNIHNCIMKGSWSQWSTWS LCTPPCSPNATRVRQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGTPRP LCEALQGQKLVVEEKRSCLHVPVCKDPEEKKP TSRS Domain of 406 SEQ ID NO: 401 VDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQD IRHCYSIQHCP domain PCIRRNMKSISCQEIPGQQSRGR binding domain KSISC TSRS Domain of 409 mouse SEQ ID VNGEWEAWGKWSDCSRLRMSINCEGTPGQQSRSRSCGGRKFNGKPCAGKLQDI NO: 405 HNCI Table 2 Examples of C5 Antibodies, Fabs and C5 Proteins -_CDRH1 410 -_CDRH2 411 -_CDRH3 412 -—CDRL1 413 -_CDRLZ 414 -_CDRL3 415 PCT/IBZOIZ/057394 41 6 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQG RVTITADESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSS 41 7 PLSVSVALGQTARITCSGDSIPNYYVYWYQQKPGQAPVLVIYDDSNRPSGIPERFSGS NSGN TATLTISRAQAGDEADYYCQSFDSSLNAEVFGGGTKLTVL 41 8 EVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIGPFFGTANYAQKFQG RVTITADESTSTAYMELSSLRSEDTAVYYCARDTPYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKST SGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVN HKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDP EVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTISKA KGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Light chain 41 9 SYELTQPLSVSVALGQTARITCSGDSI PNYYVYWYQQKPGQAPVLVIYDDSNRPSGlPERFSGSNSGN TATLTISRAQAGDEADYYCQSFDSSLNAEVFGGGTKLTVLGQF’KAAPSVTLFPPSSEELQANKATLVC PGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGST VEKTVAPTECS PN encoding 420 SEQ ID CAATTGGTTCAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCT NO:416 GCAAAGCCTCCGGAGGCACTTTTTCTTCTFATGCCATTTCTTGGGTGCGCCAAGCCCCTGGGCAG GGTCTCGAGTGGATGGGCGGTATCGGTCCGTITTITGGCACTGCGAATTACGCGCAGAAGTTTCA GGGCCGGGTGACCATTACCGCGGATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTG CGTAGCGAAGATACGGCCGTGTATTATTGCGCGCGTGATACTCCTTA'I'T'ITGATI'ATI'GGGGCCA AGGCACCCTGGTGACGGTTAGCTCA PN encoding 421 SEQ ID TCCTATGAACTCACACAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCCGGATCACCT NO:417 GCTCCGGCGACAGCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCC CGTGCTGGTGATCTACGACGACAGCAACCGGCCCAGCGGCATCCCCGAGCGG'ITCAGCGGCAG CAACAGCGGCAACACCGCCACCCTGACCATTTCCAGAGCACAGGCAGGCGACGAGGCCGACTA CTACTGCCAGAGCTTCGACAGCAGCCTGAACGCCGAGGTGTTCGGCGGAGGGACCAAGTTAACC GTCCTA PN encoding 422 SEQ ID GAGGTGCAA'ITGGTI'CAGTCTGGCGCGGAAGTGAAAAAACCGGGCAGCAGCGTGAAAGTGAGCT NO:418 GCAAAGCCTCCGGAGGCACTTTTTCTTCTTATGCCATTTCTI'GGGTGCGCCAAGCCCCTGGGCAG GGTCTCGAGTGGATGGGCGGTATCGGTCCGTTTTTTGGCACTGCGAATTACGCGCAGAAGTTTCA GGGCCGGGTGACCATTACCGCGGATGAAAGCACCAGCACCGCGTATATGGAACTGAGCAGCCTG CGTAGCGAAGATACGGCCGTGTATTATTGCGCGCGTGATACTCCTTA'H‘ITGATTATTGGGGCCA CCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCC TCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCG AACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTG TCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGG CACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGG GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAG GTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGG ACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC GGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC CCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTAC AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA PN encoding 423 SEQ ID TCCTATGAACTCACACAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCCGGATCACCT NO:419 GCTCCGGCGACAGCATCCCCAACTACTACGTGTACTGGTACCAGCAGAAGCCCGGCCAGGCCCC CGTGCTGGTGATCTACGACGACAGCAACCGGCCCAGCGGCATCCCCGAGCGGTTCAGCGGCAG CAACAGCGGCAACACCGCCACCCTGACCATTTCCAGAGCACAGGCAGGCGACGAGGCCGACTA CTACTGCCAGAGCTTCGACAGCAGCCTGAACGCCGAGGTGTTCGGCGGAGGGACCAAGTTAACC GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTC AAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC CTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAG CAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGA AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAAT GTTCA Optimized PN 424 encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAGAAGCCCGGTAGCAGCGTCAAGGTGTCC |DNOA18 TGCAAGGCCAGCGGCGGCACCTTCAGCAGCTACGCCATCAGCTGGGTGCGGCAGGCCCCAGGC CAGGGCCTGGAGTGGATGGGCGGCATCGGCCCATTCTTCGGCACCGCCAACTACGCCCAGAAG TTCCAGGGCAGGGTCACCATCACCGCCGACGAGAGCACCAGCACCGCCTACATGGAGCTGTCCA GCCTGAGAAGCGAGGACACCGCCGTGTACTACTGCGCCAGAGACACCCCCTACTTCGACTACTG GGGCCAGGGCACCCTGGTGACCGTGAGCAGCGCTAGCACCAAGGGCCCCAGCGTGTTCCCCCT GGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGACTA CTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTT CGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAG CAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGAC AAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAA GCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCA GGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCA ACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTACA CCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGA GTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGCC AAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGG AGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCA GCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAG CTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCC GGCAAG Optimized PN 425 encoding SEQ AGCTACGAGCTGACCCAGCCCCTGAGCGTGAGCGTGGCCCTGGGCCAGACCGCCAGGATCACC ID NO:419 TGCAGCGGCGACAGCATCCCCAACTACTACGTGTACTGGTATCAGCAGAAGCCCGGCCAGGCCC CCGTGCTGGTGATCTACGACGACAGCAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCA GCAACAGCGGCAACACCGCCACCCTGACCATCAGCAGAGCCCAGGCCGGCGACGAGGCCGACT ACTACTGCCAGAGCTTCGACAGCTCACTGAACGCCGAGGTGTTCGGCGGAGGGACCAAGCTGAC CGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCT GCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTG AAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAG AGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACA 2012/057394 GGTCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCG AGTGCAGC Antibody 8110 Sequence Identifier (SEQ ID NO:) and Sequence or comments -_CDRH1 426 -_CDRHZ 427 -_CDRH3 428 -_CDRL1 429 -—CDRL2 430 -—CDRL3 431 VH 432 SGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTEYSPSFQGQV TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDN |GNSWHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCGTYDIESYVFGGGTKLTVL EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGI |DPDDSYTEYSPSFQGQV S ISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTS GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNH KPSNTKVDKRVEPKSCDKTHTCF’PCPAPEAAGGPSVFLFF’PKPKDTLMISRTPEVTCVWDVSHEDPE VKFNWYVDGVEVH NAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK QVYTLPPSREEMTKNQVSLTCLVKGFYPSDlAVEWESNGQPENNYKTI'PPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Light chain 435 SYELTQPPSVSVAPGQTARISCSGDN |GNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCGTYDIESWFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLIS DFYPGAVTVAWKADSSPVKAGVETTTPSKQSN NKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVE KTVAPTECS PN encoding 436 SEQ ID GAGGTGCAATTGGTI'CAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT N02432 GCAAAGGTTCCGGATATTCCTTTACTAATTATATTTC'ITGGGTGCGCCAGATGCCTGGGAAGGGT CTCGAGTGGATGGGCATTATTGATCCTGATGATTCTI'ATACTGAGTATTCTCCTTC'TTTTCAGGGT CAGGTCACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGC GAGCGATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGGT'ITTGATATI'TGGGGCCAAG GCACCCTGGTGACGGTTAGCTCA PN encoding 437 SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:434 GTAGCGGCGATAATATTGGTAATI‘C'ITATGTI'CATTGGTACCAGCAGAAACCCGGGCAGGCGCCA GTTCTTGTGATTTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATI'ATTA‘IT GCGGTACTI'ATGATATTGAGTC‘ITATGTGTTTGGCGGCGGCACGAAG'I'I'AACCGTCCTA PN ng 488 SEQ ID GAGGTGCAA'I'TGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT W0 093762 GCAAAGGTTCCGGATATTCCTTTACTAATTATATTTCTTGGGTGCGCCAGATGCCTGGGAAGGGT CTCGAGTGGATGGGCATTATTGATCCTGATGATTCTTATACTGAGTATTCTCCTTCTTTTCAGGGT CAGGTCACCATTAGCGCGGATAAAAGCAITAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAGC GAGCGATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGGTTTTGATATTTGGGGCCAAG GCACCCTGGTGACGGTIAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCTC CTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAA CCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTC CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCA CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGA ATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGGGA CCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGT CACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGAC GGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGG GTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGG TCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCG ACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTG ACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGC CGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAG CAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCAT GAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA PN encoding 439 SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT N02436 GTAGCGGCGATAATATTGGTAATTCTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCA GTTCTTGTGATTTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT GCGGTACTTATGATATTGAGTCTTATGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCAG CCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG CCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGA TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTAC AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCC AGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA Optimized PN 44o ng SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGC iD NO:434 TGCAAGGGCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAG GGCCTGGAGTGGATGGGCATCATCGACCCCGACGACAGCTACACCGAGTACAGCCCCAGCTTCC AGGGCCAGGTGACCATCAGCGCCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCC TGAAGGCCAGCGACACCGCCATGTACTACTGCGCCAGATACGAGTACGGCGGCTTCGACATCTG GGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCCCCT GGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGACTA CTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTT CCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAG CAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGAC AAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAA GCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCA GGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCA ACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTACA ACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGA AJACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGCC AAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGG PCT/IBZOIZ/057394 AGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCA GCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAG CTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCC GGCAAG Optimized PN 441 encoding SEQ AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGC 1D NO:435 TGCAGCGGCGACAACATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCC CCCGTGCTGGTGATCTACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGC AGCAACTCCGGCAACACCGCCACCCTGACCATCAGCGGCACCCAGGCCGAGGACGAGGCCGAC TACTACTGCGGCACCTACGACATCGAGTCATACGTGTI'CGGCGGAGGGACCAAGCTGACCGTGC TGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCTGCAGG CCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTGGCCTG GAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAGAGCAA CAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTC CTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTG CAGC Antibody 8111 Sequence Identifer (SEQ ID NO' l : ) and Sequence or comments -_CDRH1 442 -_CDRH2 443 B 444 -_CDRL1 445 -—CDRL2 446 -—CDRL3 447 VH 448 EVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLAN| DDADiKDYSPSLKSRL TISKDTSKNQWLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSS V 449 ESALTQPASVSGSPGQSITISCTGTSSDIGTYNYVSWYQQ SGNTASLTISGLQAEDEADYYCQSYDSQSIVFGGGTKLTVL Heavy chain 450 EVTLKESGPALVKPTQTLTLTCTFSGFSLSTSGGGVSWIRQPPGKALEWLANIDDADIKDYSPSLKSRL TISKDTSKNQWLTMTNMDPVDTATYYCARGPYGFDSWGQGTLVTVSSASTKGPSVFPLAPSSKSTS GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNH VDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSH EDPE VDGVEVH NAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY KSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Light chain 451 ESALTQPASVSGSPGQSiTlSCTGTSSDIGTYNYVSWYQQ SGNTASLTiSGLQAEDEADYYCQSYDSQSIVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLV CLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGS TVEKTVAPTECS PN encoding 452 SEQ ID GAGGTGACATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCT WO 93762 GTACCT'ITTCCGGATTTAGCCTGTCTAC'ITCTGGTGGTGGTGTGTC'ITGGATTCGCCAGCCGCCT GGGAAAGCCCTCGAGTGGCTGGCTAATA'ITGATGATGCTGATATTAAGGATTATI'CTCCTI'CTCTT AAGTCTCGTCTGACCATTAGCAAAGATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATG GTGGATACGGCCACCTATTATTGCGCGCGTGGTCCTI'ATGGTFTTGATTCTTGGGGCCA AGGCACCCTGGTGACGGTTAGCTCA PN encoding 453 SEQ ID GAAAGCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGT N02449 GTACGGGTACTAGCAGCGATATTGGTACTTATAATTATGTGTCTI'GGTACCAGCAGCATCCCGGG AAGGCGCCGAAACTTATGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGC GGATCCAAAAGCGGCAACACCGCGAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCG GATI'ATTATTGCCAGTCTTATGATTCTCAGTCTATTGTGTTTGGCGGCGGCACGAAGTTAACCGTC PN encoding 454 SEQ ID GAGGTGACATTGAAAGAAAGCGGCCCGGCCCTGGTGAAACCGACCCAAACCCTGACCCTGACCT NO:450 GTACCTTTTCCGGATI'TAGCCTGTCTACTTCTGGTGGTGGTGTGTC‘ITGGATTCGCCAGCCGCCT GGGAAAGCCCTCGAGTGGCTGGCTAATATTGATGATGCTGATATFAAGGATTATTCTCCTTCTCTI' AAGTCTCGTCTGACCATTAGCAAAGATACTTCGAAAAATCAGGTGGTGCTGACTATGACCAACATG GACCCGGTGGATACGGCCACCTATTATTGCGCGCGTGGTCCTTATGGTFTTGATTCTTGGGGCCA CCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTI'CCCCCTGGCACCC TCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCG TGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTG TCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGG CACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGG GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAG GTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGG TGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC GGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTFCCTCTAC AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA PN encoding 455 SEQ ID GAAAGCGCACTGACCCAGCCAGCTTCAGTGAGCGGCTCACCAGGTCAGAGCATTACCATCTCGT NO:451 GTACGGGTACTAGCAGCGATATTGGTACTTATAATTATGTGTCTTGGTACCAGCAGCATCCCGGG AAGGCGCCGAAACTTATGATTTATGATGATTCTAATCGTCCCTCAGGCGTGAGCAACCGTTTTAGC GGATCCAAAAGCGGCAACACCGCGAGCCTGACCATTAGCGGCCTGCAAGCGGAAGACGAAGCG GATTATI'ATI'GCCAGTCTTATGATTCTCAGTCTATI'GTGTTTGGCGGCGGCACGAAGTTAACCGTC CTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG CCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTG GAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAAC AACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCT ACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTC Optimized PN 456 encoding SEQ GAGGTGACCCTGAAGGAGAGCGGCCCAGCCCTGGTGAAGCCCACCCAGACCCTGACCCTGACT ID NO:448 TGCACC'ITCAGCGGCTTCAGCCTGAGCACCAGCGGAGGGGGCGTGAGCTGGATCAGGCAGCCC CCAGGTAAGGCCCTGGAGTGGCTGGCCAATATCGACGACGCCGATATCAAGGACTACAGCCCCA PCT/IBZOIZ/057394 GCCTGAAGAGCAGGCTGACCATCAGCAAGGACACCAGCAAGAACCAGGTGGTGCTGACCATGAC CAATATGGACCCCGTGGACACCGCCACCTACTACTGCGCCAGAGGCCCCTACGGCTTCGACAGC TGGGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCCC CTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGAC TACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCT TCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCA GCAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGA CAAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGA AGCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGC AGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTC AACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTAC AACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAG AATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGC CAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAA GAACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGG GAGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGC AGCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCA GCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACC CGGCAAG OpmnMedPN 457 encoding SEQ GAGAGCGCCCTGACCCAGCCCGCCAGCGTGAGCGGCAGCCCAGGCCAGTCTATCACAATCAGC ID NO:449 TGCACCGGCACCTCCAGCGATATCGGCACCTACAACTACGTGAGCTGGTATCAGCAGCACCCCG GCAAGGCCCCCAAGCTGATGATCTACGACGACAGCAACAGGCCCAGCGGCGTGAGCAACAGGTT CAGCGGCAGCAAGAGCGGCAACACCGCCAGCCTGACAATCAGCGGCCTGCAGGCCGAGGACGA GGCCGACTACTACTGCCAGAGCTACGACAGCCAGTCAATCGTGTTCGGCGGAGGGACCAAGCTG ACCGTGCTGGGCCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAG CTGCAGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGAC‘ITCTACCCAGGCGCCGTGACCG TGGCCTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGC AGAGCAACAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCA CTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAAC CGAGTGCAGC Antibody 8113 Sequence Identifier (SEQ ID NO:) and Sequence or ts CDRH1 SEQ ID NO:426 CDRHZ 458 IIDPDDSYTRYSPSFQG CDRH3 SEQ ID NO:428 CDRL1 SEQ ID NO:429 CDRLZ SEQ ID NO:430 EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGIIDPDDSYTRYSPSFQGQV TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDNIGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCATWGSEDQVFGGGTKLTVL Heavychan 462 EVQLVQSGAEVKKPGESLKISCKGSGYSFTNYISWVRQMPGKGLEWMGHDPDDSYTRYSPSFQGQV TISADKSISTAYLQWSSLKASDTAMYYCARYEYGGFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTS GGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSWTVPSSSLGTQTYICNVNH PCT/lBZOlZ/057394 KPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLM| SRTPEVTCVWDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPI E KTISKAK GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTFPPVLDSDGSFFLY SKLTVD KSRWQQGNVFSCSVMHEALH NHYTQKSLSLSPGK Light chain 463 SYELTQPPSVSVAPGQTARISCSGDN IGNSYVHWYQQKPGQAPVLVIYKDNDRPSGIPERFSGSNSG NTATLT|SGTQAEDEADYYCATWGSEDQVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCL IS DFYPGAVTVAWKADSSPVKAGVETTTPSKQSN NKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTV EKTVAPTECS PN encoding 464 SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATFAGCT N01460 GCAAAGGTTCCGGATATTCCTTTACTAATTATATFTCTTGGGTGCGCCAGATGCCTGGGAAGGGT CTCGAGTGGATGGGCATTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGCTITCAGGG ACAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAAAG ATACGGCCATGTATTATTGCGCGCGTTATGAGTATGGTGGTTTTGATATTTGGGGCCAA GGCACCCTGGTGACGGTTAGCTCA PN encoding 465 SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT N0:461 GTAGCGGCGATAATATTGGTAATI'CTTATGTTCATI'GGTACCAGCAGAAACCCGGGCAGGCGCCA GTI'CTTGTGATITATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT CTTGGGGTI'CTGAGGATCAGGTGTFTGGCGGCGGCACGAAGTTAACCGTCCTA PN encoding 466 SEQ ID GAGGTGCAATTGGTI'CAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:462 GCAAAGGTTCCGGATATTCCTTTACTAATTATA‘I'I'TCTTGGGTGCGCCAGATGCCTGGGAAGGGT CTCGAGTGGATGGGCATTATCGATCCGGATGATAGCTATACCCGTTATTCTCCGAGC‘ITTCAGGG ACAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATC'ITCAATGGAGCAGCCTGAAAG CGAGCGATACGGCCATGTATI'ATTGCGCGCGTTATGAGTATGGTGGTTITGATAT‘I’I‘GGGGCCAA GGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTGGCACCCT AGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCG AACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTFCCCGGCTG TCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTI'GGG CACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTT GAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGGG GACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAG GTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGG ACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACC GGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAA GGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCC CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCC TGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTAC AGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGC ATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA PN ng 467 SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCG‘I'I'GCACCAGGTCAGACCGCGCGTATCTCGT N02483 GTAGCGGCGATAATATI’GGTAA'I'I'CTTATGTTCATTGGTACCAGCAGAAACCCGGGCAGGCGCCA GTI'CTTGTGA'ITTATAAGGATAATGATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT GCGCTACTTGGGGTTCTGAGGATCAGGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGGTCA PCT/[32012/057394 GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG GCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG ATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTA CAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC CAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA Optimized PN 468 encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGC ID NO:462 TGCAAGGGCAGCGGCTACAGCTTCACCAACTACATCAGCTGGGTGCGGCAGATGCCCGGCAAG GGCCTGGAGTGGATGGGCATCATCGACCCCGACGACAGCTACACCAGGTACAGCCCCAGCTTCC AGGGCCAGGTGACCATCAGCGCCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCAGCC TGAAGGCCAGCGACACCGCCATGTACTACTGCGCCAGATACGAGTACGGCGGCTTCGACATCTG GGGCCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCCCCT GGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGACTA CTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACACCTT CCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAGCAG CAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTGGAC AAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCCGAA GCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCAGCA GGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGTTCA ACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGTACA ACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAAAGA ATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAGGCC AAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACCAAG AACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGTGGG AGAGCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACGGCA TCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTTCAG CTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCACCC GGCAAG Optimized PN 469 encoding SEQ AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGC iD NO:463 TGCAGCGGCGACAATATCGGCAACAGCTACGTGCACTGGTATCAGCAGAAGCCCGGCCAGGCCC CCGTGCTGGTGATCTACAAGGACAACGACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCA GCAACTCCGGCAACACCGCCACCCTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACT ACTACTGCGCCACCTGGGGCTCAGAGGACCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTGC AGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCTGCAGG CCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTGGCCTG GAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAGAGCAA CAACAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAGGTC CTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGAGTG CAGC _CDRH1 470 -_CDRHZ 471 -_CDRH3 472 -_CDRL1 473 CDRL2 474 KDNNRPS CDRL3 475 QTWDTGESGV SGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGI IDPTDSQTAYSPSFQGQ VTISADKSISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSS SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCQTWDTGESGVFGGGTKLTVL EVQLVQSGAEVKKPGESLKISCKGSGYSFTSYYIGWVRQMPGKGLEWMGIIDPTDSQTAYSPSFQGQ KSISTAYLQWSSLKASDTAMYYCARYMMRGFDHWGQGTLVTVSSASTKGPSVFPLAPSSKS TSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNV NH KPS NTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSH E DPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTI PREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTI'PPVLDSDGS LTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Light chain 479 SYELTQPPSVSVAPGQTARISCSGDSLGDYYAYWYQQKPGQAPVLVIYKDNNRPSGIPERFSGSNSG NTATLTISGTQAEDEADYYCQTWDTGESGVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLV CLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGS TVEKTVAPTECS PN encoding 480 SEQ ID GAGGTGCAA'I'I'GGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:476 GCAAAGGTTCCGGATATTCCTTTACTTCTTATTATA'ITGGTTGGGTGCGCCAGATGCCTGGGAAG GGTCTCGAGTGGATGGGCATTATTGATCCTACTGATI'CTCAGACTGCTI'ATI'CTCCTTCTTTTCAG GGTCAGGTGACCATTAGCGCGGATAAAAGCATI'AGCACCGCGTATCTI'CAATGGAGCAGCCTGAA AGCGAGCGATACGGCCATGTATTATTGCGCGCGTTATATGATGCGTGG'ITTI'GATCA'l—I'GGGGCC AAGGCACCCTGGTGACGGTTAGCTCA PN encoding 481 SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT NO:478 GTAGCGGCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCA GTTCTTGTGATTTATAAGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATI'ATT GCCAGACTTGGGATACTGGTGAGTCTGGTGTGT'ITGGCGGCGGCACGAAGTFAACCGTCCTA PN encoding 482 SEQ ID GAGGTGCAATTGGTTCAGAGCGGCGCGGAAGTGAAAAAACCGGGCGAAAGCCTGAAAATTAGCT NO:479 GCAAAGGTTCCGGATATTCCTTTACTTCTTATTATATTGGTTGGGTGCGCCAGATGCCTGGGAAG GGTCTCGAGTGGATGGGCATTATTGATCCTACTGATI'CTCAGACTGCITATTCTCCTTCTTTTCAG GGTCAGGTGACCATTAGCGCGGATAAAAGCATTAGCACCGCGTATCTTCAATGGAGCAGCCTGAA AGCGAGCGATACGGCCATGTAWTATI'GCGCGCGTTATATGATGCGTGGTTTTGATCATTGGGGCC AAGGCACCCTGGTGACGGTTAGCTCAGCCTCCACCAAGGGTCCATCGGTCTI'CCCCCTGGCACC CTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC GAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCT GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGG GCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGT TGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAAGCAGCGGGG GGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGA GGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTG 2012/057394 GACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTAC CGGGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCA AGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCC CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGC CTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGC AGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTA GCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATG CATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAA PN encoding 483 SEQ ID AGTTACGAACTGACCCAGCCGCCTTCAGTGAGCGTTGCACCAGGTCAGACCGCGCGTATCTCGT N02480 GTAGCGGCGATTCTCTTGGTGATTATTATGCTTATTGGTACCAGCAGAAACCCGGGCAGGCGCCA GTTCTTGTGATTTATAAGGATAATAATCGTCCCTCAGGCATCCCGGAACGCTTTAGCGGATCCAAC AGCGGCAACACCGCGACCCTGACCATTAGCGGCACTCAGGCGGAAGACGAAGCGGATTATTATT GCCAGACTTGGGATACTGGTGAGTCTGGTGTGTTTGGCGGCGGCACGAAGTTAACCGTCCTAGG TCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAAC AAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGG CAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAA GTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGC TGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA OpmnuedPN 484 encoding SEQ GAGGTGCAGCTGGTGCAGAGCGGAGCCGAGGTGAAAAAGCCCGGTGAGAGCCTGAAGATCAGC ID N02479 TGCAAGGGCAGCGGCTACAGCTTCACCAGCTACTACATCGGCTGGGTGCGGCAGATGCCCGGC AAGGGCCTGGAGTGGATGGGCATCATCGACCCCACCGACAGCCAGACCGCCTACAGCCCCAGC TTCCAGGGCCAGGTGACCATCAGCGCCGACAAGAGCATCAGCACCGCCTACCTGCAGTGGAGCA AGGCCAGCGACACCGCCATGTACTACTGCGCCCGGTACATGATGAGGGGCTTCGACCA CTGGGGTCAGGGCACCCTGGTGACCGTCAGCTCAGCTAGCACCAAGGGCCCCAGCGTGTTCCC CCTGGCCCCCAGCAGCAAGAGCACCTCCGGCGGCACAGCCGCCCTGGGCTGCCTGGTGAAGGA CTACTTCCCCGAGCCCGTGACCGTGTCCTGGAACAGCGGAGCCCTGACCAGCGGCGTGCACAC CTTCCCCGCCGTGCTGCAGAGCAGCGGCCTGTACAGCCTGTCCAGCGTGGTGACAGTGCCCAG CAGCAGCCTGGGCACCCAGACCTACATCTGCAACGTGAACCACAAGCCCAGCAACACCAAGGTG GACAAGAGAGTGGAGCCCAAGAGCTGCGACAAGACCCACACCTGCCCCCCCTGCCCAGCCCCC GAAGCTGCAGGCGGCCCTTCCGTGTTCCTGTTCCCCCCCAAGCCCAAGGACACCCTGATGATCA GCAGGACCCCCGAGGTGACCTGCGTGGTGGTGGACGTGAGCCACGAGGACCCAGAGGTGAAGT TCAACTGGTACGTGGACGGCGTGGAGGTGCACAACGCCAAGACCAAGCCCAGAGAGGAGCAGT ACAACAGCACCTACAGGGTGGTGTCCGTGCTGACCGTGCTGCACCAGGACTGGCTGAACGGCAA AGAATACAAGTGCAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAAAAGACCATCAGCAAG GCCAAGGGCCAGCCACGGGAGCCCCAGGTGTACACCCTGCCCCCTTCTCGGGAGGAGATGACC AAGAACCAGGTGTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCCAGCGACATCGCCGTGGAGT GCAACGGCCAGCCCGAGAACAACTACAAGACCACCCCCCCAGTGCTGGACAGCGACG GCAGCTTCTTCCTGTACAGCAAGCTGACCGTGGACAAGAGCAGGTGGCAGCAGGGCAACGTGTT CAGCTGCAGCGTGATGCACGAGGCCCTGCACAACCACTACACCCAGAAGAGCCTGAGCCTGTCA CCCGGCAAG Optimized PN 485 encoding SEQ AGCTACGAGCTGACCCAGCCCCCCAGCGTGAGCGTGGCCCCAGGCCAGACCGCCAGGATCAGC ID NO:480 TGCAGCGGCGACAGCCTGGGCGACTACTACGCCTACTGGTATCAGCAGAAGCCCGGCCAGGCC CCCGTGCTGGTGATCTACAAGGACAACAACAGGCCCAGCGGCATCCCCGAGAGGTTCAGCGGCA GCAACAGCGGCAACACCGCCACCCTGACAATCAGCGGCACCCAGGCCGAGGACGAGGCCGACT ACTACTGCCAGACCTGGGACACCGGCGAGTCAGGCGTGTTCGGCGGAGGGACCAAGCTGACCG TGCTGGGTCAGCCTAAGGCTGCCCCCAGCGTGACCCTGTTCCCCCCCAGCAGCGAGGAGCTGC AGGCCAACAAGGCCACCCTGGTGTGCCTGATCAGCGACTTCTACCCAGGCGCCGTGACCGTGGC PCT/IBZOIZ/057394 CTGGAAGGCCGACAGCAGCCCCGTGAAGGCCGGCGTGGAGACCACCACCCCCAGCAAGCAGAG CAAGTACGCCGCCAGCAGCTACCTGAGCCTGACCCCCGAGCAGTGGAAGAGCCACAG GTCCTACAGCTGCCAGGTGACCCACGAGGGCAGCACCGTGGAAAAGACCGTGGCCCCAACCGA GTGCAGC Other antibodies of the invention include those where the amino acids or nucleic acids encoding the amino acids have been d, yet have at least 60, 65, 70, 75, 80, 85, 90, or 95 percent ty to the ces described in Table 1. Some embodiments include mutant amino acid sequences wherein no more than 1, 2, 3, 4 or 5 amino acids have been mutated in the variable regions when ed with the le regions depicted in the sequence described in Table 1, while retaining substantially the same antigen binding activity.

Since each of these antibodies can bind to Factor P, the VH, VL, full length light chain, and full length heavy chain sequences (amino acid sequences and the nucleotide sequences encoding the amino acid sequences) can be "mixed and matched" to create other Factor P-binding antibodies of the invention. Such "mixed and matched" Factor P- binding dies can be tested using the binding assays known in the art (e.g., ELlSAs, and other assays described in the Example section). When these chains are mixed and matched, a VH sequence from a particular VH/VL pairing should be replaced with a structurally similar VH sequence. Likewise a full length heavy chain sequence from a particular full length heavy chain /full length light chain pairing should be replaced with a structurally similar full length heavy chain ce. Likewise, a VL sequence from a particular VH/VL pairing should be replaced with a structurally similar VL sequence.

Likewise a full length light chain sequence from a particular full length heavy chain / full length light chain pairing should be replaced with a structurally similar full length light chain sequence. Accordingly, in one aspect, the invention provides an isolated antibody or antigen binding region thereof having: a heavy chain variable domain comprising an amino acid sequence selected from the group ting of SEQ ID NOs: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 and 273, and a light chain variable domain comprising an amino acid sequence selected from the group consisting of SEQ lD NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274 wherein the antibody specifically binds to Factor P (e.g., human and/or cynomolgus Factor P).

In another , the invention provides (i) an isolated antibody having: a full length heavy chain comprising an amino acid sequence that has been optimized for expression in a ian cell selected from the group consisting of SEQ ID NOs: 9, PCT/IBZOIZ/057394 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261 and 275, and a full length light chain comprising an amino acid sequence that has been zed for expression in a mammalian cell selected from the group consisting of SEQ ID N03: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262 and 276; or (ii) a onal protein comprising an antigen binding portion The terms “complementarity ining region,” and “CDR,” as used herein refer to the sequences of amino acids within antibody variable regions which confer antigen specificity and binding ty. in general, there are three CDRs in each heavy chain variable region (HCDR1, HCDR2, HCDR3) and three CDRs in each light chain le region (LCDR1, LCDR2, LCDR3).

The precise amino acid sequence boundaries of a given CDR can be readily determined using any of a number of well-known schemes, including those bed by Kabat et al. (1991), “Sequences of ns of immunological interest,” 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD (“Kabat" numbering scheme), Al—Lazikani et al., (1997) JMB 273,927-948 (“Chothia" numbering scheme).

For example, under Kabat, the CDR amino acid residues in the heavy chain variable domain (VH) are numbered 31—35 (HCDR1), 50—65 (HCDR2), and 95-102 (HCDR3); and the CDR amino acid residues in the light chain variable domain (VL) are numbered 24—34 (LCDR1), 50—56 (LCDRZ), and 89-97 (LCDR3). Under a the CDR amino acids in the VH are numbered 26~32 (HCDR1), 52—56 (HCDR2), and 95-102 (HCDR3); and the amino acid residues in VL are numbered 26—32 (LCDR1), 50—52 (LCDRZ), and 91-96 (LCDR3). By combining the CDR definitions of both Kabat and Chothia, the CDRs consist of amino acid residues 26-35 (HCDR1), 50-65 (HCDR2), and 95~102 (HCDR3) in human VH and amino acid residues 24-34 (LCDR1), 50—56 (LCDR2), and 89-97 (LCDR3) in human VL. in another , the present invention provides Factor P binding antibodies that comprise the heavy chain and light chain CDR1s, CDR2s, and CDR3s as described in Table 1, or combinations thereof. The amino acid sequences of the VH CDR1s of the antibodies are shown in SEQ lD NOs: 1, 15, 29, 43, 57, 71, 85, 99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, or 267. The amino acid ces of the VH CDR2s of the antibodies and are shown in SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, or 268. The amino acid sequences of the VH CDR3S of the antibodies are shown in SEQ iD NOS: 3, 17, 31, 45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199, 213, 227, 241, 255, or 269. The amino acid sequences of the VL CDRts of the antibodies are shown in SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, or 270. The amino acid sequences of the VL CDRZS of the antibodies are shown in SEQ ID NOs: 5, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, or 271. The amino acid sequences of the VL CDR3s of the antibodies are shown in SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, or 272. These CDR regions are delineated using the Kabat system.

Alternatively, as defined using the Chothia system (AI-Lazikani et al., (1997) JMB 273,927-948) the amino acid sequences of the VH CDR1s of the antibodies are shown in SEQ ID NOs: 281, 287, 293, 299, 305, 311, 317, 323, 329, 335, 341, 347, 353, 359, 365, 371, 377, 383, 389, or 395. The amino acid sequences of the VH CDR2s of the antibodies and are shown in SEQ ID N08: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, or 396. The amino acid sequences of the VH CDR3s of the antibodies are shown in SEQ ID NOs: 283, 289, 295, 301, 307, 313, 319, 325, 331, 337, 343, 349, 355, 361, 367, 373, 379, 385, 391, or 397. The amino acid sequences of the VL CDR1s of the antibodies are shown in SEQ ID NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, or 398. The amino acid sequences of the VL CDR2s of the dies are shown in SEQ ID NOs: 285, 291, 297, 303, 309, 315, 321, 327, 333, 339, 345, 351, 357, 363, 369, 375, 381, 387, 393, or 399. The amino acid sequences of the VL CDR3s of the antibodies are shown in SEQ ID N03: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, or 400.

Given that each of these antibodies can bind to Factor P and that antigen-binding specificity is provided primarily by the CDR1, 2 and 3 regions, the VH CDR1, 2 and 3 sequences and VL CDR1, 2 and 3 ces can be "mixed and matched" (i.e., CDRs from different antibodies can be mixed and matched, although each antibody preferably ns a VH CDR1, 2 and 3 and a VL CDR1, 2 and 3 to create other Factor P binding g molecules of the invention. Such "mixed and matched" Factor P g antibodies can be tested using the binding assays known in the art and those bed in the Examples (e.g., ELISAs, SET, Biacore). When VH CDR sequences are mixed and matched, the CDR1, CDR2 and/or CDR3 sequence from a particular VH sequence should be replaced with a urally similar CDR sequence(s). Likewise, when VL CDR sequences are mixed and matched, the CDR1, CDR2 and/or CDR3 sequence from a particular VL sequence should be replaced with a structurally similar CDR sequence(s).

It will be readily nt to the ordinarily skilled artisan that novel VH and VL sequences can be created by tuting one or more VH and/or VL CDR region sequences with structurally r sequences from the CDR sequences shown herein for monoclonal antibodies of the present invention. In addition to the foregoing, in one embodiment, the antigen binding fragments of the antibodies described herein can comprise a VH CDR1, 2, and 3, or a VL CDR 1, 2, and 3, wherein the fragment binds to Factor P as a single variable domain.

In certain embodiments of the invention, the antibodies or n g fragments thereof may have the heavy and light chain sequences of the Fabs described in Table 1. More specifically, the antibody or antigen binding fragment thereof may have the heavy and light sequence of Fab NVSQ62, NVSQBB, NV8964, NV8965, NV8966, NV8967, NVS805, , NVS807, NVS808, NVS809, NV8962-S, NVSQSZ-Q, NVSQ62-S31A, NVSQBZ-G, NVSQ62—T, NVSQGS-S, NV8965—T, or NV8965—Q.

In other ments of the invention the antibody or antigen g fragment in that specifically binds Factor P ses a heavy chain variable region CDR1, a heavy chain variable region CDR2, a heavy chain variable region CDR3, a light chain variable region CDR1, a light chain variable region CDR2, and a light chain variable region CDR3 as defined by Kabat and described in Table 1. In still other embodiments of the invention the antibody or antigen g nt in that specifically binds Factor P ses a heavy chain variable region CDR1, a heavy chain variable region CDR2, a heavy chain variable region CDR3, a light chain variable region CDR1, a light chain variable region CDR2, and a light chain variable region CDR3 as defined by Chothia and described in Table 1.

In a specific embodiment, the ion includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO:1; a heavy chain variable region CDR2 of SEQ ID NO: 2; a heavy chain variable region CDR3 of SEQ ID NO: 3; a light chain variable region CDR1 of SEQ ID NO: 4; a light chain variable region CDR2 of SEQ ID NO: 5; and a light chain variable region CDR3 of SEQ ID NO: 6. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 15; a heavy chain variable region CDR2 of SEQ ID NO: 16; a heavy chain variable region CDR3 of SEQ ID NO: 17; a light chain variable region CDR1 of SEQ ID NO: 18; a light chain variable region CDR2 of SEQ ID NO: 19; and a light chain variable region CDR3 of SEQ ID NO: 20. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 29; a heavy chain variable region CDR2 of SEQ ID NO: 30; a heavy chain variable region CDR3 of SEQ ID NO: 31; a light chain variable region CDR1 of SEQ ID NO: 32; a light chain variable region CDR2 of SEQ ID NO: 33; and a light chain le region CDR3 of SEQ ID NO: 34. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 43; a heavy chain variable region CDR2 of SEQ ID NO: 44; a heavy chain variable region CDR3 of SEQ ID NO: 45; a light chain variable region CDR1 of SEQ ID NO: 46; a light chain variable region CDR2 of SEQ ID NO: 47; and a light chain variable region CDR3 of SEQ ID NO: 48. In another specific embodiment, the invention es an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 57; a heavy chain le region CDR2 of SEQ ID NO: 58; a heavy chain variable region CDR3 of SEQ ID NO: 59; a light chain variable region CDR1 of SEQ ID NO: 60; a light chain variable region CDR2 of SEQ ID NO: 61; and a light chain le region CDR3 of SEQ ID NO: 62. In another specific embodiment, the invention es an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 71; a heavy chain variable region CDR2 of SEQ ID NO: 72; a heavy chain variable region CDR3 of SEQ ID NO: 73; a light chain variable region CDR1 of SEQ ID NO: 74; a light chain variable region CDR2 of SEQ ID NO: 75; and a light chain variable region CDR3 of SEQ ID NO: 76. In another specific ment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 85; a heavy chain variable region CDR2 of SEQ ID NO: 86; a heavy chain variable region CDR3 of SEQ ID NO: 87; a light chain variable region CDR1 of SEQ ID NO: 88; a light chain variable region CDR2 of SEQ ID NO: 89; and a light chain le region CDR3 of SEQ ID NO: 90. In r specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 99; a heavy chain variable region CDR2 of SEQ ID NO: 100; a heavy chain variable region CDR3 of SEQ ID NO: 101; a light chain variable region CDR1 of SEQ ID NO: 102; a light chain variable region CDR2 of SEQ ID NO: 103; and a light chain variable region CDR3 of SEQ ID NO: 104. In another specific embodiment, the ion includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 113; a heavy chain variable region CDR2 of SEQ ID NO: 114; a heavy chain variable region CDR3 of SEQ ID NO: 115; a light chain variable region CDR1 of SEQ ID NO: 116; a light chain variable region CDR2 of SEQ ID NO: 117; and a light chain le region CDR3 of SEQ ID NO: 118. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 ofSEQ ID NO: 127; a heavy chain variable region CDR2 of SEQ ID NO: 128; a heavy chain variable region CDR3 of SEQ ID NO: 129; a light chain variable region CDR1 of SEQ ID NO: 130; a light chain le region CDR2 of SEQ ID NO: 131; and a light chain variable region CDR3 of SEQ ID NO: 132. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 141; a heavy chain variable region CDR2 of SEQ ID NO: 142; a heavy chain variable region CDR3 of SEQ ID NO: 143; a light chain variable region CDR1 of SEQ ID NO: 144; a light chain variable region CDR2 of SEQ ID NO: 145; and a light chain le region CDR3 of SEQ ID NO: 146. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 155; a heavy chain variable region CDR2 of SEQ ID NO: 156; a heavy chain variable region CDR3 of SEQ ID NO: 157; a light chain variable region CDR1 of SEQ ID NO: 158; a light chain variable region CDR2 of SEQ ID NO: 159; and a light chain le region CDR3 of SEQ ID NO: 160. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain le region CDR1 of SEQ ID NO: 169; a heavy Chain variable region CDR2 of SEQ ID NO: 170; a heavy chain variable region CDR3 ofSEQ ID NO: 171; a light chain variable region CDR1 of SEQ ID NO: 172; a light chain variable region CDR2 of SEQ ID NO: 173; and a light chain variable region CDR3 of SEQ ID NO: 174. In another specific ment, the ion includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 183; a heavy chain variable region CDR2 of SEQ ID NO: 184; a heavy chain variable region CDR3 of SEQ ID NO: 185; a light chain variable region CDR1 of SEQ ID NO: 186; a light chain le region CDR2 of SEQ ID NO: 187; and a light chain variable region CDR3 of SEQ ID NO: 188. In another specific embodiment, the invention includes an antibody that ically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 197; a heavy chain variable region CDR2 of SEQ ID NO: 198; a heavy chain variable region CDR3 of SEQ ID NO: 199; a light chain variable region CDR1 of SEQ ID NO: 200; a light chain variable region CDR2 of SEQ ID NO: 201; and a light chain variable region CDR3 of SEQ ID NO: 202. In another specific embodiment, the invention includes an dy that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 211; a heavy chain variable region CDR2 of SEQ ID NO: 212; a heavy chain variable region CDR3 of SEQ ID NO: 213; a light chain variable region CDR1 of SEQ ID NO: 214; a light chain variable region CDR2 of SEQ ID NO: 215; and a light chain variable region CDR3 of SEQ ID NO: 216. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 225; a heavy chain variable region CDR2 of SEQ ID NO: 226; a heavy chain variable region CDR3 of SEQ ID NO: 227; a light chain variable region CDR1 of SEQ ID NO: 228; a light chain variable region CDR2 of SEQ ID NO: 229; and a light chain variable region CDR3 of SEQ ID NO: 230. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 239; a heavy chain variable region CDR2 of SEQ ID NO: 240; a heavy chain variable region CDR3 of SEQ ID NO: 241; a light chain variable region CDR1 of SEQ ID NO: 242; a light chain variable region CDR2 of SEQ ID NO: 243; and a light chain variable region CDR3 of SEQ ID NO: 244. In r specific embodiment, the ion includes an dy that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 253; a heavy chain variable region CDR2 of SEQ ID NO: 254; a heavy chain variable region CDR3 of SEQ ID NO: 255; a light chain variable region CDR1 of SEQ ID NO: 256; a light chain variable region CDR2 of SEQ ID NO: 257; and a light chain variable region CDR3 of SEQ ID NO: 258. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 267; a heavy chain variable region CDR2 of SEQ ID NO: 268; a heavy chain variable region CDR3 of SEQ ID NO: 269; a light chain le region CDR1 of SEQ ID NO: 270; a light chain variable region CDR2 of SEQ ID NO: 271; and a light chain le region CDR3 of SEQ ID NO: 271.

In another specific embodiment, the invention includes an antibody that specifically binds to Factor P sing a heavy chain variable region CDR1 of SEQ ID NO: 281; a heavy chain variable region CDR2 of SEQ ID NO: 282; a heavy chain variable region CDR3 of SEQ ID NO: 283; a light chain variable region CDR1 of SEQ ID NO: 284; a light chain variable region CDR2 ofSEQ ID NO: 285; and a light chain variable region CDR3 of SEQ ID NO: 286. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 287; a heavy chain variable region CDR2 of SEQ ID NO: 288; a heavy chain variable region CDR3 of SEQ ID NO: 289; a light chain variable region CDR1 of SEQ ID NO: 290; a light chain variable region CDR2 of SEQ ID NO: 291; and a light chain variable region CDR3 of SEQ ID NO: 292. In another specific embodiment, the invention includes an dy that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 293; a heavy chain variable region CDR2 of SEQ ID NO: 294; a heavy chain variable region CDR3 of SEQ ID NO: 295; a light chain variable region CDR1 of SEQ ID NO: 296; a light chain variable region CDR2 of SEQ ID NO: 297; and a light chain variable region CDR3 of SEQ ID NO: 298. In another specific embodiment, the invention es an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 299; a PCT/IBZOIZIOS7394 heavy chain variable region CDR2 of SEQ ID NO: 300; a heavy chain variable region CDR3 of SEQ ID NO: 301; a light chain variable region CDR1 of SEQ ID NO: 302; a light chain variable region CDR2 of SEQ ID NO: 303; and a light chain variable region CDR3 of SEQ ID NO: 304. In another specific embodiment, the invention includes an dy that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 305; a heavy chain variable region CDR2 of SEQ ID NO: 306; a heavy chain variable region CDR3 of SEQ ID NO: 307; a light chain le region CDR1 of SEQ ID NO: 308; a light chain variable region CDR2 of SEQ ID NO: 309; and a light chain variable region CDR3 of SEQ ID NO: 310. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 311; a heavy chain variable region CDR2 of SEQ ID NO: 312; a heavy chain variable region CDR3 of SEQ ID NO: 313; a light chain variable region CDR1 of SEQ ID NO: 314; a light chain le region CDR2 of SEQ ID NO: 315; and a light chain variable region CDR3 of SEQ ID NO: 316. In another specific embodiment, the invention includes an dy that specifically binds to Factor P comprising a heavy chain le region CDR1 of SEQ ID NO: 317; a heavy chain variable region CDR2 of SEQ ID NO: 318; a heavy chain variable region CDR3 of SEQ ID NO: 319; a light chain variable region CDR1 of SEQ ID NO: 320; a light chain variable region CDR2 of SEQ ID NO: 321; and a light chain variable region CDR3 of SEQ ID NO: 322. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 323; a heavy chain variable region CDR2 of SEQ ID NO: 324; a heavy chain variable region CDR3 of SEQ ID NO: 325; a light chain variable region CDR1 of SEQ ID NO: 326; a light chain variable region CDR2 of SEQ ID NO: 327; and a light chain le region CDR3 of SEQ ID NO: 328. In another specific ment, the invention es an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 329; a heavy chain variable region CDR2 of SEQ ID NO: 330; a heavy chain variable region CDR3 of SEQ ID NO: 331; a light chain variable region CDR1 of SEQ ID NO: 332; a light chain variable region CDR2 of SEQ ID NO: 333; and a light chain variable region CDR3 of SEQ ID NO: 334. In r specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 335; a heavy chain variable region CDR2 of SEQ ID NO: 336; a heavy chain variable region CDR3 ofSEQ ID NO: 337; a light chain variable region CDR1 of SEQ ID NO: 338; a light chain variable region CDR2 of SEQ ID NO: 339; and a light chain variable region CDR3 of SEQ ID NO: 340. In another specific embodiment, the invention es an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 ofSEQ ID NO: 341; a heavy chain 2012/057394 variable region CDR2 of SEQ ID NO: 342; a heavy chain le region CDR3 of SEQ ID NO: 343; a light chain variable region CDR1 of SEQ ID NO: 344; a light chain variable region CDR2 of SEQ ID NO: 345; and a light chain variable region CDR3 of SEQ ID NO: 346. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 347; a heavy chain variable region CDR2 of SEQ ID NO: 348; a heavy chain variable region CDR3 of SEQ ID NO: 349; a light chain le region CDR1 of SEQ ID NO: 350; a light chain variable region CDR2 of SEQ ID NO: 351; and a light chain variable region CDR3 of SEQ ID NO: 352. In another specific embodiment, the ion includes an antibody that specifically binds to Factor P comprising a heavy chain le region CDR1 of SEQ ID NO: 353; a heavy chain variable region CDR2 of SEQ ID NO: 354; a heavy chain variable region CDR3 of SEQ ID NO: 355; a light chain variable region CDR1 of SEQ ID NO: 356; a light chain variable region CDR2 of SEQ ID NO: 357; and a light chain variable region CDR3 of SEQ ID NO: 358. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 359; a heavy chain variable region CDR2 of SEQ ID NO: 360; a heavy chain le region CDR3 of SEQ ID NO: 361; a light chain variable region CDR1 of SEQ ID NO: 362; a light chain variable region CDR2 of SEQ ID NO: 363; and a light chain variable region CDR3 of SEQ ID NO: 364. In r specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 365; a heavy chain variable region CDR2 of SEQ ID NO: 366; a heavy chain variable region CDR3 of SEQ ID NO: 367; a light chain variable region CDR1 of SEQ ID NO: 368; a light chain variable region CDR2 of SEQ ID NO: 369; and a light chain variable region CDR3 of SEQ ID NO: 370. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 371; a heavy chain variable region CDR2 of SEQ ID NO: 372; a heavy chain variable region CDR3 of SEQ ID NO: 373; a light chain variable region CDR1 of SEQ ID NO: 374; a light chain variable region CDR2 of SEQ ID NO: 375; and a light chain variable region CDR3 of SEQ ID NO: 376. In another specific embodiment, the invention includes an antibody that specifically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 377; a heavy chain variable region CDR2 of SEQ ID NO: 378; a heavy chain variable region CDR3 of SEQ ID NO: 379; a light chain variable region CDR1 of SEQ ID NO: 380; a light chain le region CDR2 of SEQ ID NO: 381; and a light chain variable region CDR3 of SEQ ID NO: 382. In another specific embodiment, the invention includes an dy that cally binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 383; a heavy chain variable region CDR2 of PCT/IBZOIZ/057394 SEQ ID NO: 384; a heavy chain variable region CDR3 of SEQ ID NO: 385; a light chain variable region CDR1 of SEQ ID NO: 386; a light chain variable region CDR2 of SEQ ID NO: 387; and a light chain variable region CDR3 of SEQ iD NO: 388. In another specific embodiment, the invention includes an antibody that ically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 389; a heavy chain variable region CDR2 of SEQ ID NO: 390; a heavy chain variable region CDR3 of SEQ ID NO: 391; a light chain le region CDR1 of SEQ ID NO: 392; a light chain variable region CDR2 of SEQ ID NO: 393; and a light chain variable region CDR3 of SEQ ID NO: 394. In another specific embodiment, the invention includes an antibody that ically binds to Factor P comprising a heavy chain variable region CDR1 of SEQ ID NO: 395; a heavy chain variable region CDR2 of SEQ ID NO: 396; a heavy chain le region CDR3 of SEQ ID NO: 397; a light chain variable region CDR1 of SEQ ID NO: 398; a light chain variable region CDR2 of SEQ ID NO: 399; and a light chain variable region CDR3 of SEQ ID NO: 400.

In certain embodiments, the invention includes antibodies or n binding fragments that specifically binds to Factor P as described in Table 1. In a red embodiment, the antibody, or antigen binding fragment, that binds Factor P is Fab NVS962, , , NV8965, NV8966, NV8967, NV8804, NV8805, NVSBOG, , NV8808, NV8809, NVS962-S, NV8962-Q, NVS962-G, NV8962—T, NV8962- 831A, NV8965-T, NVS965—Q, or NVS965-S.

As used herein, a human antibody comprises heavy or light chain variable s or full length heavy or light chains that are "the product of" or "derived from" a particular germline sequence if the variable regions or full length chains of the antibody are obtained from a system that uses human germline immunoglobulin genes. Such systems include immunizing a transgenic mouse carrying human immunoglobulin genes with the antigen of st or screening a human immunoglobulin gene library displayed on phage with the antigen of interest. A human antibody that is "the product of" or "derived from" a human germline immunoglobulin sequence can be identified as such by comparing the amino acid ce of the human antibody to the amino acid sequences of human germline immunoglobulins and selecting the human germline immunoglobulin sequence that is closest in sequence (i.e., greatest % identity) to the sequence of the human antibody. A human antibody that is "the product of" or "derived from” a particular human germline immunoglobulin sequence may contain amino acid differences as compared to the germline sequence, due to, for example, naturally ing somatic mutations or intentional introduction of site-directed mutations. However, in the VH or VL framework regions, a selected human dy typically is at least 90% identical in amino acids sequence to an amino acid sequence encoded by a human germline immunoglobulin gene and contains amino acid residues that identify the human antibody as being human when compared to the germline immunoglobulin amino acid sequences of other species (e.g., murine germline sequences). In certain cases, a human antibody may be at least 60%, 70%, 80%, 90%, or at least 95%, or even at least 96%, 97%, 98%, or 99% identical in amino acid sequence to the amino acid sequence encoded by the germline immunoglobulin gene. Typically, a recombinant human antibody will display no more than 10 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene in the VH or VL framework regions. In certain cases, the human antibody may display no more than 5, or even no more than 4, 3, 2, or 1 amino acid difference from the amino acid ce d by the germline globulin gene. Examples of human germline immunoglobulin genes include, but are not limited to the le domain germline fragments described below, as well as DP47 and DPK9.

Homologous antibodies In yet another embodiment, the present invention provides an antibody, or an n binding fragment thereof, comprising amino acid sequences that are homologous to the sequences described in Table 1, and the antibody binds to a Factor P n (9.9., human and/or cynomolgus Factor P), and retains the desired functional properties of those antibodies described in Table 1.

For example, the invention es an ed antibody, or a functional antigen binding fragment thereof, sing a heavy chain variable domain and a light chain variable , wherein the heavy chain variable domain comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group ting of SEQ ID NOs: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 and 273; the light chain variable domain comprises an amino acid sequence that is at least 80%, at least 90%, or at least 95% identical to an amino acid sequence selected from the group consisting of SEQ lD NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274; and the antibody specifically binds to Factor P (e.g., human and/or cynomolgus Factor P). in other embodiments, the VH and/or VL amino acid sequences may be 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1. In other embodiments, the VH and/or VL amino acid sequences may be PCT/182012/057394 identical except for an amino acid substitution in no more than 1,2,3,4 or 5 amino acid positions. An antibody having VH and VL regions having high (i. e., 80% or greater) identity to the VH and VL regions of those described in Table 1 can be obtained by mutagenesis (e.g., site-directed or PCR-mediated mutagenesis) of nucleic acid molecules encoding SEQ ID NOs: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 or 273 and SEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274, tively, followed by testing of the d altered antibody for retained function using the functional assays described herein.

In other embodiments, the full length heavy chain and/or full length light chain amino acid sequences may be 50% 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1. An antibody having a full length heavy chain and full length light chain having high (i.e., 80% or r) identity to the full length heavy chains of any of SEQ ID NOs : 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261 or 275, and full length light chains of any of SEQ ID NOs 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276, can be obtained by mutagenesis (e.g., site-directed or FOR-mediated nesis) of nucleic acid molecules encoding such polypeptides, followed by testing of the encoded altered antibody for ed function using the functional assays described herein.

In other ments, the full length heavy chain and/or full length light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1.

In other ments, the variable regions of heavy chain and/or the variable regions of light chain nucleotide sequences may be 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98% or 99% identical to the sequences set forth in Table 1.

As used herein, the percent identity between the two sequences is a function of the number of identical positions shared by the ces (i.e., % identity equals number of identical positions/total number of positions x 100), taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences. The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm, as bed in the non-limiting examples below. 2012/057394 Additionally or alternatively, the protein ces of the present invention can further be used as a "query sequence" to perform a search against public databases to, for e, identify related sequences. For example, such searches can be performed using the BLAST program (version 2.0) of Altschul, et a/., 1990 J.Mol. Biol. 215:403-10.

Antibodies with Conservative Modifications In certain embodiments, an antibody of the invention has a heavy chain variable region comprising CDR1, CDR2, and CDR3 sequences and a light chain variable region comprising CDR1, CDR2, and CDR3 sequences, wherein one or more of these CDR sequences have specified amino acid sequences based on the antibodies described herein or conservative modifications thereof, and wherein the dies retain the desired functional properties of the Factor P-binding antibodies of the invention.

Accordingly, the invention provides an ed antibody, or a antigen binding fragment f, consisting of a heavy chain variable region comprising CDR1, CDR2, and CDR3 sequences and a light chain variable region comprising CDR1, CDR2, and CDR3 sequences, wherein: the heavy chain variable region CDR1 amino acid ces are selected from the group consisting of SEQ iD NOs: 1, 15, 29, 43, 57, 71, 85, 99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, and 267, and conservative modifications thereof; the heavy chain variable region CDR2 amino acid sequences are ed from the group consisting ofSEQ lD NOs: 2, 16, 30, 44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268, and conservative modifications thereof; the heavy chain variable region CDR3 amino acid sequences are selected from the group consisting ofSEQ iD NOs: 3, 17, 31, 45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199, 213, 227, 241, 255, and 269, and vative modifications thereof; the light chain variable regions CDR1 amino acid sequences are selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270, and conservative modifications thereof; the light chain variable regions CDR2 amino acid sequences are selected from the group consisting of SEQ lD NOs: 5, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271, and conservative modifications f; the light chain le regions of CDR3 amino acid sequences are selected from the group consisting of SEQ ID NOS: 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272, and conservative modifications thereof; and the antibody or antigen binding fragment thereof specificaiiy binds to Factor P.

WO 93762 PCT/132012/057394 in other ments, the antibody of the invention is optimized for expression in a mammalian cell has a full length heavy chain sequence and a full length light chain sequence, wherein one or more of these sequences have ied amino acid ces based on the antibodies described herein or conservative modifications thereof, and wherein the antibodies retain the desired functional properties of the Factor P binding antibodies of the invention. Accordingly, the invention provides an isolated antibody optimized for expression in a mammalian cell consisting of a full length heavy chain and a full length light chain wherein the full length heavy chain has amino acid sequences ed from the group of SEQ ID NOs: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261 and 275, and conservative modifications thereof; and the full length light chain has amino acid sequences selected from the group of SEQ lD NOs: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, and 276, and vative modifications thereof; and the antibody ically binds to Factor P (e.g., human and/or cynomolgus Factor P).

Antibodies That Bind to the Same Epitope The present invention provides antibodies that bind to the same epitope as the Factor P binding antibodies described in Table 1. Additional dies can therefore be fied based on their ability to compete (e.g., to competitively inhibit the binding of, in a statistically significant manner) with other antibodies of the invention in Factor P binding assays (such as those described in the Examples). The ability of a test antibody to inhibit the binding of antibodies of the present invention to a Factor P n demonstrates that the test antibody can compete with that antibody for binding to Factor P; such an antibody may, according to non-limiting theory, bind to the same or a related (9.9., a structurally similar or spatially proximal) epitope on the Factor P protein as the antibody with which it es. in a certain embodiment, the antibody that binds to the same epitope on Factor P as the dies of the present invention is a human monoclonal antibody. Such human monoclonal dies can be prepared and isolated as described herein. As used herein, an antibody “competes" for binding when the competing antibody inhibits Factor P binding of an antibody or n binding fragment of the invention by more than 50%, in the presence of an equimolar concentration of competing antibody.

In other embodiments the antibodies or antigen binding fragments of the invention bind the Thrombospondin type 5 repeat (TSR 5) domain of Factor P (SEQ ID NO: 406). In other embodiments the antibodies or antigen binding fragments of the WO 93762 PCT/IBZOIZ/057394 invention bind a region of the Factor P TSR5 domain comprising SEQ ID NO: 407. Still in other embodiments the region comprises SEQ ID NO: 408.

In other embodiments of the ion the isolated antibodies or antigen binding fragments bind an epitope comprising SEQ ID NO: 407, and in other embodiments the epitope comprises SEQ ID NO: 408. In other embodiments of the invention, the antibodies or antigen binding fragments bind a peptide according to SEQ ID NO: 407 and in still other embodiments the Factor P epitope includes SEQ ID NO: 408.

Engineered and Modified dies An antibody of the Invention r can be prepared using an antibody having one or more of the VH and/or VL sequences shown herein as starting material to engineer a modified antibody, which modified antibody may have altered properties from the starting antibody. An antibody can be engineered by modifying one or more residues within one or both variable regions (i. e., VH and/or VL), for example within one or more CDR regions and/or within one or more framework regions. Additionally or alternatively, an antibody can be ered by modifying residues within the constant region(s), for example to alter the effector function(s) of the antibody.

One type of variable region engineering that can be med is CDR grafting.

Antibodies interact with target antigens predominantly through amino acid residues that are located in the six heavy and light chain complementarity determining regions .

For this reason, the amino acid sequences within CDRs are more diverse n individual antibodies than sequences outside of CDRs. Because CDR ces are responsible for most antibody-antigen interactions, it is le to express recombinant antibodies that mimic the properties of specific naturally occurring antibodies by constructing expression vectors that include CDR sequences from the specific naturally ing antibody d onto framework sequences from a different antibody with different properties (see, 9.9., Riechmann, L. et al., 1998 Nature 332:323-327; Jones, P. etal., 1986 Nature 321:522—525; Queen, C. etal., 1989 Proc. Natl. Acad., U.S.A. 29—10033; US. Patent No. 5,225,539 to Winter, and US. Patent Nos. 5,530,101; ,585,089; 5,693,762 and 6,180,370 to Queen at al.) Accordingly, another embodiment of the invention pertains to an isolated antibody, or an antigen binding fragment thereof, comprising a heavy chain variable region comprising CDR1 ces having an amino acid sequence selected from the group ting of SEQ ID NOs: 1, 15, 29, 43, 57, 71, 85, 99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, and 267; CDR2 sequences having an amino acid PCT/[32012/057394 sequence selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268; CDR3 ces having an amino acid ce selected from the group consisting of SEQ ID NOs: 3, 17, 31, 45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199, 213, 227, 241, 255, and 269, respectively; and a light chain variable region having CDR1 sequences having an amino acid ce selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270; CDR2 ces having an amino acid sequence selected from the group consisting of SEQ ID NOs25, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271; and CDR3 sequences consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272, respectively. Thus, such antibodies contain the VH and VL CDR sequences of monoclonal antibodies, yet may contain different framework sequences from these antibodies.

Alternatively, another embodiment of the invention pertains to an isolated antibody, or an n binding fragment thereof, comprising a heavy chain variable region comprising CDR1 ces having an amino acid sequence selected from the group consisting of SEQ ID N03: 281, 287, 293, 299, 305, 311, 317, 323, 329, 335, 341, 347, 353, 359, 365, 371, 377, 383, 389, and 395; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID N03: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, and 396; CDR3 ces having an amino acid sequence selected from the group consisting of SEQ ID N03: 283, 289, 295, 301, 307, 313, 319, 325, 331, 337, 343, 349, 355, 361, 367, 373, 379, 385, 391, and 397, respectively; and a light chain le region having CDR1 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, and 398; CDR2 sequences having an amino acid sequence selected from the group consisting of SEQ ID NOs: 285, 291, 297, 303, 309, 315, 321, 327, 333, 339, 345, 351, 357, 363, 369, 375, 381, 387, 393, and 399; and CDR3 sequences consisting of an amino acid ce selected from the group consisting of SEQ ID NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, and 400, respectively. Thus, such antibodies contain the VH and VL CDR sequences of monoclonal antibodies, yet may contain different framework sequences from these antibodies.

Such framework sequences can be obtained from public DNA databases or published references that include germline antibody gene sequences. For example, germline DNA sequences for human heavy and light chain variable region genes can be found in the “VBase” human germline sequence database (available on the world wide web at mrc- cpe.cam.ac.uk/vbase), as well as in Kabat, E. A., et al., 1991 Sequences of Proteins of Immunological interest, Fifth Edition, U.S. Department of Health and Human Services, NIH Publication No. 91-3242; Tomlinson, l. M., eta/., 1992 J. Mol. Biol. 227:776-798; and Cox, J. P. L. et al., 1994 Eur. J lmmunol. 24:827—836; the contents of each of which are expressly incorporated herein by reference.

An example of framework sequences for use in the antibodies of the invention are those that are urally similar to the framework sequences used by selected antibodies of the invention, e.g., consensus sequences and/or framework sequences used by monoclonal antibodies of the invention. The VH CDR1, 2 and 3 sequences, and the VL CDR1, 2 and 3 sequences, can be grafted onto framework regions that have the identical sequence as that found in the germline immunoglobulin gene from which the framework sequence derive, or the CDR sequences can be grafted onto framework regions that contain one or more mutations as compared to the germline sequences. For example, it has been found that in n instances it is cial to mutate residues within the framework regions to maintain or enhance the antigen binding ability of the antibody (see 6.9., US. Patent Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al). Frameworks that can be utilized as scaffolds on which to build the dies and antigen binding fragments described herein e, but are not limited to VH1A, VH1 B, VH3, Vk1, Vl2, and Vk2. onal frameworks are known in the art and may be found, for example, in the vBase data base on the world wide web at vbase.mrc~ m.ac.uk/index.ph p?&MMN_position=1 :1 .

Accordingly, an embodiment of the invention relates to isolated Factor P binding antibodies, or antigen g fragments thereof, comprising a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ lD NOs: 7, 21, 35, 49, 63, 77, 91, 105, 119,133,147,161,175, 189, 203, 217, 231, 245, 259 and 273, or an amino acid sequence having one, two, three, four or five amino acid tutions, deletions or additions in the framework region of such sequences, and further comprising a light chain variable region having an amino acid sequence selected from the group consisting ofSEQ ID NOs: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, and 274, or an amino acid sequence having one, two, three, four or five amino acid tutions, deletions or additions in the framework region of such sequences.

PCT/IBZOIZ/057394 r type of variable region ation is to mutate amino acid residues within the VH and/or VL CDR1, CDR2 and/or CDR3 regions to thereby improve one or more binding properties (e.g., ty) of the antibody of interest, known as “affinity maturation." Site-directed nesis or PCR-mediated mutagenesis can be performed to introduce the mutation(s) and the effect on antibody binding, or other functional property of st, can be evaluated in in vitro or in vivo assays as described herein and provided in the Examples. Conservative modifications (as sed above) can be introduced. The ons may be amino acid substitutions, additions or deletions.

Moreover, typically no more than one, two, three, four or five residues within a CDR region are altered.

Accordingly, in another embodiment, the invention provides isolated Factor P- binding antibodies, or antigen binding fragments f, consisting of a heavy chain variable region having a VH CDR1 region consisting of an amino acid sequence selected from the group having SEQ lD NOs: 1, 15,29, 43, 57, 71, 85,99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, and 267 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or ons as compared to SEQ lD NOs: 1, 15, 29, 43, 57, 71, 85, 99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, or 267; a Vl-l CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 16, 30, 44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 268 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 2, 16, 30,44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212,226, 240, 254, or 268; a VH CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 17, 31, 45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199, 213, 227, 241, 255, and 269, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ lD NOs: 3, 17, 31,45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199,213,227, 241, 255, or 269; a VL CDR1 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or ons as compared to SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228,242, 256, or 270; a VL CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 5, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: PCT/IBZOIZ/057394 , 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, or 271; and a VL CDR3 region having an amino acid ce selected from the group ting of SEQ ID NOS: 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID NOs: 6, 20, 34,48, 62, 76,90, 104, 118, 132, 146, 160, 174, 188, 202, 216,230,244, 258, or 272.

Accordingly, in another embodiment, the invention provides isolated Factor P— binding antibodies, or antigen binding fragments thereof, consisting of a heavy chain variable region having a VH CDR1 region consisting of an amino acid sequence selected from the group having SEQ ID N03: 281, 287, 9, 305, 311, 317, 323, 329, 335, 341, 347, 353, 359, 365, 371, 377, 383, 389, and 395 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID N08: 281, 287, 293, 299, 305, 311, 317, 323, 329, 335, 341, 347, 353, 359, 365, 371, 377, 383, 389, or 395; a VH CDR2 region having an amino acid ce selected from the group consisting of SEQ ID N03: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, and 396 or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID N03: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, or 396; a VH CDR3 region having an amino acid sequence selected from the group consisting of SEQ ID NOs: 283, 289, 295, 301, 307, 313, 319, 325, 331, 337, 343, 349, 355, 361, 367, 373, 379, 385, 391, and 397, or an amino acid sequence having one, two, three, four or five amino acid substitutions, ons or additions as compared to SEQ ID N03: 283, 289, 295, 301, 307, 313, 319, 325, 331, 337, 343, 349, 355, 361, 367, 373, 379, 385, 391, or 397; a VL CDR1 region having an amino acid sequence selected from the group consisting of SEQ ID N08: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, and 398, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or ons as compared to SEQ ID N03: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, or 398; a VL CDR2 region having an amino acid sequence selected from the group consisting of SEQ ID N03: 285, 291, 297, 303, 309, 315, 321, 327, 333, 339, 345, 351, 357, 363, 369, 375, 381, 387, 393, and 399, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID N03: 285, 291, 297, 303, 309, 315, 321, 327, 333, 339, 345, 351, 357, 363, 369, 375, 381, 387, 393, or 399; and a VL CDR3 region having an amino acid sequence selected from the group consisting of SEQ lD N03: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, and 400, or an amino acid sequence having one, two, three, four or five amino acid substitutions, deletions or additions as compared to SEQ ID N08: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, or 400.

Grafting Antigen-binding Domains Into Alternative Frameworks or Scaffolds A wide variety of antibody/ globulin frameworks or scaffolds can be employed so long as the resulting polypeptide includes at least one binding region which specifically binds to Factor P. Such frameworks or scaffolds include the 5 main idiotypes of human immunoglobulins, or fragments thereof, and include immunoglobulins of other animal s, preferably having humanized aspects. Single heavy-chain antibodies such as those identified in camelids are of particular interest in this regard. Novel frameworks, scaffolds and fragments continue to be discovered and developed by those skilled in the art.

In one aspect, the invention pertains to generating non-immunoglobulin based dies using non- immunoglobulin scaffolds onto which CDRs of the ion can be grafted. Known or future non-immunoglobulin frameworks and scaffolds may be employed, as long as they comprise a binding region specific for the target Factor P protein. Known non-immunoglobulin orks or scaffolds include, but are not limited to, fibronectin (Compound Therapeutics, Inc., Waltham, MA), ankyrin (Molecular Partners AG, Zurich, rland), domain antibodies (Domantis, Ltd., Cambridge, MA, and Ablynx nv, Zwijnaarde, Belgium), lipocalin (Pieris Proteolab AG, Freising, Germany), small modular immuno-pharmaceuticals (Trubion Pharmaceuticals lnc., Seattle, WA), maxybodies (Avidia, lnc., Mountain View, CA), n A (Affibody AG, Sweden), and affilin -crystallin or ubiquitin) (Scil Proteins GmbH, Halle, Germany).

The fibronectin scaffolds are based on fibronectin type lll domain (e.g., the tenth module of the fibronectin type ill (10 Fn3 domain». The fibronectin type III domain has 7 or 8 beta strands which are distributed between two beta sheets, which themselves pack against each other to form the core of the protein, and further containing loops (analogous to CDRs) which connect the beta strands to each other and are solvent exposed. There are at least three such loops at each edge of the beta sheet sandwich, where the edge is the ry of the n perpendicular to the direction of the beta strands (see US 6,818,418). These fibronectin-based lds are not an immunoglobulin, although the l fold is closely related to that of the smallest PCT/[32012/057394 functional dy fragment, the variable region of the heavy chain, which comprises the entire antigen ition unit in camel and llama lgG. Because of this ure, the non-immunoglobulin antibody mimics antigen binding properties that are r in nature and affinity to those of antibodies. These scaffolds can be used in a loop randomization and shuffling strategy in vitro that is similar to the process of ty maturation of antibodies in vivo. These ectin-based les can be used as scaffolds where the loop regions of the molecule can be replaced with CDRs of the invention using standard cloning techniques.

The ankyrin technology is based on using ns with ankyrin derived repeat modules as scaffolds for bearing variable regions which can be used for binding to different targets. The ankyrin repeat module is a 33 amino acid polypeptide consisting of two anti-parallel o-helices and a B-turn. Binding of the variable regions is mostly optimized by using ribosome y.

Avimers are d from l A—domain containing protein such as LRP—1.

These domains are used by nature for protein—protein interactions and in human over 250 ns are structurally based on A—domains. Avimers consist of a number of different “A—domain" monomers (2-10) linked via amino acid linkers. Avimers can be created that can bind to the target antigen using the methodology described in, for example, U.S. PatentApplication Publication Nos. 20040175756; 53973; 20050048512; and 20060008844.

Affibody affinity ligands are small, simple ns composed of a three—helix bundle based on the scaffold of one of the lgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold domain consists of 58 amino acids, 13 of which are randomized to generate affibody libraries with a large number of ligand variants (See e.g., US 5,831,012). Affibody molecules mimic antibodies, they have a molecular weight of 6 kDa, compared to the molecular weight of antibodies, which is 150 kDa. in spite of its small size, the binding site of affibody molecules is similar to that of an antibody.

Anticalins are products developed by the y Pieris ProteoLab AG. They are derived from lipocalins, a widespread group of small and robust proteins that are usually involved in the physiological transport or storage of chemically sensitive or insoluble compounds. Several natural lipocalins occur in human tissues or body liquids.

The protein architecture is reminiscent of immunoglobulins, with hypervariable loops on top of a rigid framework. r, in contrast with antibodies or their recombinant fragments, lipocalins are composed of a single ptide chain with 160 to 180 amino acid es, being just marginally bigger than a single immunoglobulin domain. The set of four loops, which makes up the binding pocket, shows nced structural plasticity and tolerates a variety of side chains. The g site can thus be reshaped in a proprietary process in order to recognize prescribed target molecules of different shape with high affinity and specificity. One protein of lipocalin family, the bilin—binding protein (BBP) of Pieris cae has been used to develop anticalins by mutagenizing the set of four loops. One example of a patent application describing anticalins is in PCT Publication No. WO 199916873.

Affilin molecules are small non-immunoglobulin proteins which are designed for specific affinities towards proteins and small molecules. New affilin molecules can be very quickly ed from two libraries, each of which is based on a different human derived scaffold protein. Affilin molecules do not show any structural homology to immunoglobulin ns. Currently, two affilin scaffolds are ed, one of which is gamma crystalline, a human structural eye lens protein and the other is "ubiquitin" superfamily proteins. Both human scaffolds are very small, show high temperature stability and are almost resistant to pH changes and denaturing agents. This high stability is mainly due to the expanded beta sheet structure of the proteins. Examples of gamma crystalline derived proteins are described in W0200104‘l44 and examples of "ubiquitin—like" proteins are bed in W02004106368.

Protein epitope mimetics (PEM) are medium~sized, cyclic, peptide-like molecules (MW ) mimicking airpin secondary structures of proteins, the major secondary structure involved in protein-protein interactions.

The present invention provides fully human antibodies that specifically bind to a Factor P protein. Compared to the chimeric or humanized antibodies, the human Factor P—binding antibodies of the invention have r reduced antigenicity when administered to human subjects.

Camelid antibodies Antibody proteins ed from members of the camel and dromedary (Camelus bactrianus and Calelus dromaderius) family including new world members such as llama s (Lama paccos, Lama glama and Lama vicugna) have been characterized with respect to size, structural complexity and antigenicity for human subjects. Certain lgG antibodies from this family of s as found in nature lack light chains, and are thus structurally distinct from the typical four chain quaternary structure having two heavy and two light chains, for dies from other animals. See PCT/EP93/02214 (WO 94/04678 published 3 March 1994).

A region of the camelid dy which is the small single variable domain identified as VHH can be obtained by genetic engineering to yield a small protein having high affinity for a target, resulting in a low molecular weight antibody-derived protein known as a “camelid nanobody”. See U.S. patent number 5,759,808 issued June 2, 1998; see also Stijlemans, B. etal., 2004 J Biol Chem 279: 1256-1261; Dumoulin, M. et al., 2003 Nature 424: 783—788; Pleschberger, M. et al. 2003 Bioconjugate Chem 14: 440— 448; —Retamozo, V. et al. 2002 lnt J Cancer 89: 456-62; and Lauwereys, M. eta]. 1998 EMBO J 17: 3512-3520. Engineered libraries of camelid antibodies and antibody fragments are commercially available, for example, from Ablynx, Ghent, Belgium. As with other dies of non-human , an amino acid ce of a camelid antibody can be altered recombinantly to obtain a sequence that more closely resembles a human sequence, i.e., the nanobody can be “humanized”. Thus the natural low antigenicity of camelid antibodies to humans can be further reduced.

The camelid nanobody has a molecular weight approximately one-tenth that of a human lgG molecule, and the n has a physical diameter of only a few nanometers.

One consequence of the small size is the ability of d nanobodies to bind to antigenic sites that are functionally invisible to larger antibody proteins, i.e., camelid nanobodies are useful as reagents detect antigens that are otherwise cryptic using classical immunological ques, and as possible eutic agents. Thus yet another consequence of small size is that a camelid nanobody can inhibit as a result of binding to a specific site in a groove or narrow cleft of a target protein, and hence can serve in a capacity that more closely resembles the function of a cal low molecular weight drug than that of a classical antibody.

The low molecular weight and compact size further result in camelid nanobodies being extremely thermostable, stable to extreme pH and to proteolytic digestion, and poorly antigenic. Another consequence is that camelid nanobodies readily move from the circulatory system into tissues, and even cross the blood-brain r and can treat disorders that affect nen/ous tissue. Nanobodies can further facilitated drug transport across the blood brain barrier. See US. patent application 20040161738 published August 19, 2004. These features combined with the low antigenicity to humans te great eutic ial. Further, these molecules can be fully expressed in prokaryotic cells such as E. coli and are expressed as fusion proteins with bacteriophage and are functional.

WO 93762 ZOIZ/057394 Accordingly, a feature of the present ion is a camelid antibody or nanobody having high y for Factor P. In certain embodiments herein, the camelid antibody or nanobody is naturally produced in the camelid animal, i.e., is produced by the camelid following immunization with Factor P or a peptide fragment thereof, using techniques described herein for other antibodies. Alternatively, the Factor P—binding camelid nanobody is ered, i.e., produced by selection for example from a library of phage displaying appropriately mutagenized camelid nanobody proteins using panning procedures with Factor P as a target as bed in the examples herein. Engineered nanobodies can further be customized by genetic engineering to have a half life in a recipient subject of from 45 minutes to two weeks. in a specific embodiment, the camelid antibody or nanobody is obtained by grafting the CDRs sequences of the heavy or light chain of the human dies of the invention into nanobody or single domain antibody framework sequences, as bed for example in PCT/EP93/02214.

Bispecific Molecules and Multivalent Antibodies In another aspect, the present ion features bispecific or multispecific les comprising a Factor P-binding dy, or a fragment thereof, of the invention. An antibody of the invention, or antigen—binding regions thereof, can be derivatized or linked to r functional molecule, e.g., another peptide or n (9.9., r antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules. The antibody of the invention may in fact be derivatized or linked to more than one other functional molecule to te multi-specific molecules that bind to more than two ent binding sites and/or target molecules; such multi-specific molecules are also intended to be encompassed by the term "bispecific molecule" as used herein. To create a bispecific molecule of the invention, an antibody of the invention can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, peptide or binding mimetic, such that a bispecific molecule results.

Accordingly, the present invention includes bispecific molecules comprising at least one first binding specificity for Factor P and a second binding specificity for a second target epitope. For example, the second target epitope is another epitope of Factor P different from the first target epitope.

Additionally, for the invention in which the ific molecule is multi-specific, the molecule can further include a third binding specificity, in on to the first and second target epitope. in one embodiment, the bispecific molecules of the ion comprise as a binding city at least one antibody, or an antibody fragment thereof, ing, e.g., a Fab, Fab', 2, Fv, or a single chain Fv. The antibody may also be a light chain or heavy chain dimer, or any minimal fragment f such as a Fv or a single chain construct as described in Ladner et al. US Patent No. 4,946,778.

Diabodies are bivalent, ific molecules in which VH and VL domains are expressed on a single polypeptide chain, connected by a linker that is too short to allow for pairing between the two domains on the same chain. The VH and VL domains pair with complementary domains of another chain, thereby creating two antigen binding sites (see e.g., Holliger etaL, 1993 Proc. Natl. Acad. Sci. USA 90:6444—6448; Poljak etal., 1994 Structure 2:1121-1123). Diabodies can be produced by expressing two polypeptide chains with either the structure VHA-VLB and A (VH-VL configuration), or VLA~VHB and VLB-VHA (VL—VH uration) within the same cell.

Most of them can be expressed in soluble form in bacteria. Single chain diabodies (scDb) are produced by connecting the two diabody—forming polypeptide chains with linker of approximately 15 amino acid es (see Holliger and Winter, 1997 Cancer lmmunol. |mmunother., 45(3-4):128—30; Wu et a/., 1996 lmmunotechnology, 2(1):.21-36). scDb can be expressed in bacteria in soluble, active ric form (see Holliger and Winter, 1997 Cancer Immunol. |mmunother., : 128-30; Wu eta!., 1996 lmmunotechnology, 2(1):21-36; Pluckthun and Pack, 1997 lmmunotechnology, 3(2): 83— 105; Ridgway etal., 1996 Protein Eng, 17—21). A diabody can be fused to Fc to generate a “di—diabody” (see Lu et al., 2004 J. Biol. Chem., 279(4):2856-65).

Other antibodies which can be employed in the bispecific molecules of the invention are murine, chimeric and humanized monoclonal antibodies.

Bispecific molecules can be prepared by conjugating the constituent binding specificities, using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then ated to one another.

When the binding specificities are proteins or peptides, a variety of coupling or cross— linking agents can be used for covalent conjugation. Examples of cross-linking agents include protein A, carbodiimide, N-succinimidyl—S-acetyl-thioacetate (SATA), 5,5'- dithiobis(2—nitrobenzoic acid) (DTNB), o-phenylenedimaleimide (oPDM), N—succinimidyl— PCT/[32012/057394 3-(2—pyridyldithio)propionate , and sulfosuccinimidyl 4—(N-maleimidomethyl) cyclohaxane-l-carboxylate (sulfo-SMCC) (see 9.9., Karpovsky et al., 1984 J. Exp. Med. 86; Liu, MA et a/., 1985 Proc. Natl. Acad. Sci. USA 8). Other methods include those described in Paulus, 1985 Behring Ins. Mitt. No. 78,118—132; Brennan et al., 1985 Science 229281-83), and Glennie et al., 1987 J. Immunol. 139: 2367—2375). ating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co.

(Rockford, lL).

When the binding specificities are antibodies, they can be conjugated by sulfhydryl bonding of the C-terminus hinge regions of the two heavy chains. In a particularly embodiment, the hinge region is modified to contain an odd number of sulfhydryl residues, for example one, prior to conjugation.

Alternatively, both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell. This method is particularly useful where the bispecific le is a mAb X mAb, mAb X Fab, Fab x F(ab‘)2 or ligand x Fab fusion protein. A bispecific molecule of the invention can be a single chain le comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two g inants. Bispecific molecules may se at least two single chain molecules. Methods for ing bispecific molecules are described for example in US. Patent Number 5,260,203; US. Patent Number ,455,030; U.S. Patent Number 4,881,175; U.S. Patent Number 5,132,405; US. Patent Number 5,091,513; US. Patent Number 5,476,786; U.S. Patent Number 5,013,653; U.S.

Patent Number 5,258,498; and US. Patent Number 5,482,858.

Binding of the bispecific molecules to their specific targets can be confirmed by, for e, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (REA), FACS analysis, bioassay (e.g., growth inhibition), or Western Blot assay. Each of these assays generally detects the presence of protein—antibody complexes of particular interest by ing a labeled reagent (e.g., an antibody) specific for the complex of interest.

In another aspect, the present invention provides multivalent compounds comprising at least two identical or different antigen-binding portions of the antibodies of the invention binding to Factor P. The antigen-binding portions can be linked together via protein fusion or covalent or non covalent linkage. Alternatively, s of linkage have been described for the bispecfic molecules. Tetravalent compounds can be obtained for example by cross—linking dies of the antibodies of the invention with 1 00 an antibody that binds to the nt regions of the antibodies of the invention, for example the F0 or hinge region.

Trimerizing domain are described for example in Borean patent EP 1 012 28081.

Pentamerizing modules are bed for e in PCT/EP97/05897.

Antibodies with Extended Half Life The t invention provides for antibodies that specifically bind to Factor P protein which have an extended half—life in vivo.

Many factors may affect a protein’s half life in vivo. For examples, kidney filtration, metabolism in the liver, degradation by proteolytlc enzymes ases), and immunogenic responses (e.g., protein neutralization by antibodies and uptake by macrophages and dentritic cells). A variety of strategies can be used to extend the half life of the antibodies of the present invention. For example, by chemical linkage to polyethyleneglycol (PEG), reCODE PEG, antibody ld, polysialic acid (PSA), hydroxyethyl starch (HES), n-binding ligands, and carbohydrate shields; by genetic fusion to proteins g to serum proteins, such as albumin, lgG, FcRn, and transferring; by coupling (genetically or chemically) to other binding es that bind to serum proteins, such as nanoboies, Fabs, DARPins, avimers, affibodies, and anticalins; by genetic fusion to rPEG, n, domain of albumin, albumin-binding proteins, and F0; or by incorporation into nancarriers, slow release formulations, or medical devices.

To prolong the serum circulation of antibodies in vivo, inert polymer molecules such as high molecular weight PEG can be attached to the antibodies or a fragment thereof with or without a multifunctional linker either through site-specific conjugation of the PEG to the N- or C-terminus of the antibodies or via epsilon-amino groups present on lysine residues. To pegylate an antibody, the antibody, or fragment thereof, typically is reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions in which one or more PEG groups become attached to the antibody or antibody fragment. The pegylation can be carried out by an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous ve water-soluble polymer). As used herein, the term "polyethylene " is intended to encompass any of the forms of PEG that have been used to derivatize other proteins, such as mono (Ct-C10) - or aryloxy—polyethylene glycol or polyethylene glycol- maleimide. In certain ments, the antibody to be pegylated is an aglycosylated antibody. Linear or ed polymer derivatization that results in minimal loss of ical activity will be used. The degree of conjugation can be closely monitored by 1 01 SDS—PAGE and mass spectrometry to ensure proper conjugation of PEG molecules to the antibodies. Unreacted PEG can be separated from antibody-PEG conjugates by size-exclusion or by ion-exchange chromatography. PEG-derivatized antibodies can be tested for binding activity as well as for in vivo efficacy using methods well-known to those of skill in the art, for e, by immunoassays described . Methods for pegylating proteins are known in the art and can be applied to the antibodies of the ion. See for example, EP 0 154 316 by Nishimura et al. and EP 0 401 384 by lshikawa et al.

Other modified pegylation technologies include reconstituting ally onal directed engineering technology E PEG), which incorporates chemically specified side chains into biosynthetic ns via a reconstituted system that es tRNA synthetase and tRNA. This technology enables incorporation of more than 30 new amino acids into biosynthetic proteins in E.coli, yeast, and mammalian cells.

The tRNA incorporates a nonnative amino acid any place an amber codon is positioned, converting the amber from a stop codon to one that s oration of the chemically specified amino acid.

Recombinant pegylation technology (rPEG) can also be used for serum halflife extension. This technology involves genetically fusing a 300-600 amino acid unstructured protein tail to an existing pharmaceutical protein. Because the apparent molecular weight of such an unstructured protein chain is about 15-fold larger than its actual molecular weight, the serum halflife of the protein is greatly increased. In contrast to ional PEGyIation, which requires chemical conjugation and repurification, the manufacturing process is greatly simplified and the product is homogeneous.

Polysialytion is another technology, which uses the natural polymer polysialic acid (PSA) to prolong the active life and improve the stability of therapeutic peptides and proteins. PSA is a polymer of siaiic acid (a sugar). When used for n and therapeutic peptide drug delivery, polysialic acid es a protective microenvironment on conjugation. This increases the active life of the therapeutic protein in the circulation and prevents it from being recognized by the immune system. The PSA r is naturally found in the human body. It was adopted by certain bacteria which d over millions of years to coat their walls with it. These naturally polysialylated bacteria were then able, by virtue of molecular mimicry, to foil the body’s defense system. PSA, nature's te stealth technology, can be easily produced from such bacteria in large quantities and with predetermined physical characteristics. Bacterial PSA is completely 1 02 non-immunogenic, even when coupled to proteins, as it is chemically identical to PSA in the human body. r technology includes the use of hydroxyethyl starch (“HES”) derivatives linked to antibodies. HES is a modified natural poiymer derived from waxy maize starch and can be metabolized by the body’s s. HES ons are usually administered to substitute deficient blood volume and to improve the rheological ties of the blood. Hesylation of an antibody enables the prolongation of the ation half-life by increasing the stability of the molecule, as welt as by ng renal clearance, resulting in an increased biological activity. By varying different parameters, such as the molecular weight of HES, a wide range of HES dy conjugates can be customized.

Antibodies having an sed half-life in vivo can also be generated introducing one or more amino acid modifications (i.e., substitutions, insertions or deletions) into an IgG constant domain, or FcRn binding fragment f (preferably a F0 or hinge Fc domain fragment). See, e.g., International Publication No. WO 98/23289; international Publication No. WO 31; and US. Patent No. 6,277,375.

Further, antibodies can be conjugated to albumin (e.g., human serum albumin; HSA) in order to make the antibody or antibody fragment more stable in vivo or have a longer half life in vivo. The techniques are well—known in the art, see, e.g., International Publication Nos. WO 93/15199, WO 93/15200, and WO 01/77137; and European Patent No. EP 413,622. In addition, in the context of a bispecific antibody as described above, the specificities of the dy can be designed such that one binding domain of the antibody binds to Factor P while a second binding domain of the antibody binds to serum albumin, ably HSA.

The strategies for increasing half life is especially useful in nanobodies, fibronectin-based binders, and other antibodies or proteins for which increased in vivo half life is desired.

Antibody Conjugates The present invention provides antibodies or fragments thereof that specifically bind to a Factor P protein recombinantly fused or chemically conjugated (including both covalent and non-covalent conjugations) to a heterologous protein or polypeptide (or fragment thereof, preferably to a polypeptide of at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90 or at least 100 amino acids) to generate fusion proteins. in particular, the invention es fusion proteins PCT/IBZOIZ/057394 1 03 comprising an antigen-binding nt of an antibody described herein (6.9., a Fab fragment, Fd fragment, Fv fragment, F(ab)2 nt, a VH domain, a VH CDR, a VL domain or a VL CDR) and a heteroiogous protein, polypeptide, or peptide. Methods for fusing or conjugating proteins, polypeptides, or peptides to an antibody or an antibody fragment are known in the art. See, e.g., US. Patent Nos. 5,336,603, 5,622,929, ,359,046, 5,349,053, 5,447,851, and 5,112,946; European Patent Nos. EP 307,434 and EP 367,166; international Publication Nos. WO 96/04388 and WO 91/06570; Ashkenazi et a/., 1991, Proc. Natl. Acad. Sci. USA 88: 10535-10539; Zheng et al., 1995, J. immunoi. 154:5590-5600; and Vii etal., 1992, Proc. Natl. Acad. Sci. USA 89:11337— 11341.

Additional fusion proteins may be ted through the techniques of gene- shuffiing, motif—shuffling, exon-shuffiing, and/or codon—shuffling (collectively ed to as “DNA shuffling”). DNA shuffling may be employed to alter the ties of antibodies of the invention or fragments thereof (e.g., dies or fragments thereof with higher affinities and lower dissociation rates). See, generally, US. Patent Nos. 5,605,793, ,811,238, 5,830,721, 5,834,252, and 5,837,458; Patten eta/., 1997, Curr. Opinion Biotechnol. 8:724—33; Harayama, 1998, Trends Biotechnol. 16(2):?6—82; Hansson, eta/., 1999, J. Moi. Biol. 287:265-76; and Lorenzo and , 1998, Biotechniques 24(2):308- 313 (each of these patents and publications are hereby orated by reference in its entirety). Antibodies or fragments thereof, or the encoded antibodies or fragments thereof, may be altered by being subjected to random mutagenesis by prone PCR, random nucleotide insertion or other methods prior to recombination. A polynucleotide encoding an antibody or fragment thereof that specifically binds to a Factor P protein may be ined with one or more ents, motifs, sections, parts, domains, fragments, etc. of one or more heteroiogous molecules.

Moreover, the antibodies or fragments f can be fused to marker sequences, such as a peptide to facilitate purification. in preferred embodiments, the marker amino acid sequence is a hexa—histidine peptide, such as the tag provided in a pQE vector (QIAGEN, inc., 9259 Eton , Chatsworth, CA, 91311), among others, many of which are commercially available. As described in Gentz et at, 1989, Proc.

Natl. Acad. Sci. USA 86:821-824, for instance, hexa-histidine provides for convenient purification of the fusion n. Other peptide tags useful for purification include, but are not limited to, the hemaggiutinin (“HA”) tag, which corresponds to an epitope derived from the influenza hemaggiutinin n (Wilson et a/., 1984, Cell 372767), and the “flag" tag. 1 04 In other embodiments, antibodies of the present invention or fragments f conjugated to a diagnostic or detectable agent. Such dies can be useful for monitoring or sing the onset, development, progression and/or severity of a disease or disorder as part of a clinical testing procedure, such as determining the efficacy of a particular therapy. Such diagnosis and detection can accomplished by coupling the antibody to detectable substances including, but not limited to, various enzymes, such as, but not limited to, horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase; prosthetic groups, such as, but not limited to, streptavidinlbiotin and avidin/biotin; fluorescent materials, such as, but not limited to, umbelliferone, fluorescein, scein isothiocynate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; luminescent materials, such as, but not limited to, luminol; bioluminescent materials, such as but not limited to, luciferase, luciferin, and aequorin; radioactive materials, such as, but not limited to, iodine (131i, 125i, 123i, and 12“,), carbon (14C), sulfur (358), tritium (3H), indium (115In, 113m, 112ln, and 111ln,), technetium (99Tc), thallium (201Ti), gallium (6863, 676a), palladium ), molybdenum (99Mo), xenon (133Xe), fluorine (18F), 1538m, 177Lu, 159C5d, 149Pm, 140La, 175Yb, 166Ho, 90Y, 4780, 186Re, 188Re,142 Pr, 105Rh, 97Ru, 686e, 5700, 65Zn, 858r, 32P, 15SGd, 169Yb, 51Cr, 54Mn, 7589, 113Sn, and 117Tin; and positron emitting metals using various on emission tomographies, and noradioactive gnetic metal ions.

The present invention further encompasses uses of antibodies or fragments thereof conjugated to a therapeutic moiety. An antibody or fragment thereof may be conjugated to a therapeutic moiety such as a cytotoxin, e.g., a cytostatic or dal agent, a therapeutic agent or a radioactive metal ion, e.g., alpha-emitters. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells.

Further, an antibody or fragment thereof may be ated to a therapeutic moiety or drug moiety that modifies a given biological response. Therapeutic moieties or drug moieties are not to be construed as limited to classical al therapeutic .

For example, the drug moiety may be a protein, peptide, or polypeptide possessing a d biological activity. Such proteins may include, for e, a toxin such as abrin, ricin A, pseudomonas exotoxin, cholera toxin, or diphtheria toxin; a protein such as tumor is , rferon, B-interferon, nerve growth factor, platelet derived growth factor, tissue plasminogen activator, an apoptotic agent, an anti-angiogenic agent; or, a biological response modifier such as, for example, a lymphokine.

WO 93762 1 05 Moreover, an antibody can be conjugated to therapeutic moieties such as a radioactive metal ion, such as alph-emiters such as 213Bi or macrocyclic chelators useful for conjugating radiometal ions, including but not limited to, 131ln, 131LU, 131Y, 131 Ho, 131Sm, to polypeptides. In certain ments, the macrocyclic or is —tetraazacyclododecane—N,N’,N”,N”’-tetraacetic acid (DOTA) which can be attached to the antibody via a linker molecule. Such linker molecules are commonly known in the art and described in o et al., 1998, Clin Cancer Res. 2483-90; Peterson et al., 1999, Bioconjug. Chem. 10(4):553-7; and Zimmerman etal., 1999, Nucl.

Med. Biol. 26(8):943-50, each orated by reference in their entireties.

Techniques for conjugating therapeutic moieties to antibodies are well known, see, e.g., Arnon et al., “Monoclonal Antibodies For lmmunotargeting Of Drugs In Cancer Therapy”, in Monoclonal Antibodies And Cancer y, Reisfeld et al. , pp. 243- 56 (Alan R. Liss, Inc. 1985); Hellstrom etal., “Antibodies For Drug Delivery”, in Controlled Drug ry (2nd Ed), Robinson et al. (eds.), pp. 623-53 (Marcel Dekker, Inc. 1987); Thorpe, “Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review”, in Monoclonal dies 84: Biological And Clinical Applications, Pinchera et al. (eds), pp. 475-506 (1985); “Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy”, in Monoclonal Antibodies For Cancer Detection And Therapy, Baldwin et al. (eds), pp. 303-16 (Academic Press 1985), and Thorpe et al., 1982, Immunol. Rev. 621119—58.

Antibodies may also be attached to solid supports, which are particularly useful for assays or purification of the target antigen. Such solid supports include, but are not limited to, glass, cellulose, polyacrylamide, nylon, polystyrene, polyvinyl chloride or polypropylene.

Methods of Producing Antibodies of the Invention Nucleic Acids Encoding the Antibodies The invention provides substantially purified nucleic acid molecules which encode polypeptides comprising segments or domains of the Factor P -binding dy chains described above. Some of the nucleic acids of the invention comprise the nucleotide sequence encoding the heavy chain variable region shown in SEQ ID NO: 7, 21, 35, 49, 63, 77, 91, 105, 119, 133, 147, 161, 175, 189, 203, 217, 231, 245, 259 or 273, and/or the nucleotide sequence encoding the light chain variable region shown in SEQ ID NO: 8, 22, 36, 50, 64, 78, 92, 106, 120, 134, 148, 162, 176, 190, 204, 218, 232, 246, 260, or 274. In a specific embodiment, the nucleic acid molecules are those identified in Table PCT/IBZOIZ/057394 1 06 1. Some other nucleic acid molecules of the invention comprise nucleotide sequences that are substantially cal (e.g., at least 65, 80%, 95%, or 99%) to the nucleotide sequences of those identified in Table 1. When sed from riate sion vectors, polypeptides encoded by these polynucleotides are capable of exhibiting Factor P antigen binding capacity.

Also provided in the invention are polynucleotides which encode at least one CDR region and usually all three CDR regions from the heavy or light chain of the Factor P-binding antibody set forth above. Some other cleotides encode all or substantially all of the variable region sequence of the heavy chain and/or the light chain of the Factor P-binding antibody set forth above. Because of the degeneracy of the code, a variety of nucleic acid sequences will encode each of the immunoglobulin amino acid sequences.

The nucleic acid molecules of the invention can encode both a variable region and a constant region of the dy. Some of nucleic acid sequences of the invention comprise nucleotides encoding a mature heavy chain sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature heavy chain sequence set forth in SEQ lD NO: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261 or 275. Some other nucleic acid sequences comprising tide encoding a mature light chain sequence that is substantially identical (e.g., at least 80%, 90%, or 99%) to the mature light chain sequence set forth in SEQ ID NO: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, or 276.

The polynucleotide sequences can be produced by de novo solid-phase DNA synthesis or by PCR mutagenesis of an existing sequence (e.g., sequences as described in the Examples below) encoding a Factor P—binding antibody or its binding fragment.

Direct chemical synthesis of nucleic acids can be accomplished by s known in the art, such as the phosphotriester method of Narang et al., 1979, Meth. Enzymol. 68:90; the phosphodiester method of Brown et al., Meth. Enzymol. 682109, 1979; the diethylphosphoramidite method of Beaucage et al., Tetra. Lett., 22:1859, 1981; and the solid support method of US. Patent No. 4,458,066. Introducing mutations to a polynucleotide sequence by PCR can be performed as described in, e.g., PCR Technology: Principles and Applications for DNA Amplification, H.A. Erlich (Ed.), Freeman Press, NY, NY, 1992; PCR Protocols: A Guide to Methods and Applications, Innis etal. (Ed), Academic Press, San Diego, CA, 1990; Mattila eta!., Nucleic Acids Res. , 1991; and Eckert et al., PCR Methods and Applications 1:17, 1991.

PCT/IBZOIZ/057394 1 07 Also provided in the invention are expression vectors and host cells for ing the Factor P-binding antibodies described above. Various expression vectors can be employed to express the poiynucleotides encoding the Factor ing antibody chains or binding fragments. Both viral-based and nonviral expression vectors can be used to e the antibodies in a ian host cell. Nonviral s and systems include plasmids, episomal vectors, typically with an sion cassette for expressing a protein or RNA, and human artificial chromosomes (see, e.g., Harrington eta/., Nat Genet :345, 1997). For example, nonviral vectors useful for expression of the Factor P- g polynucleotides and polypeptides in mammalian (e.g., human) cells include pThioHis A, B & C, pcDNA3.1/His, pEBVHis A, B & C, (lnvitrogen, San Diego, CA), MPSV vectors, and numerous other vectors known in the art for expressing other proteins. Useful viral vectors include vectors based on retroviruses, adenoviruses, adenoassociated viruses, herpes viruses, vectors based on SV40, papilloma virus, HBP Epstein Barr virus, vaccinia virus vectors and Semliki Forest virus (SFV). See, Brent et a/., supra; Smith, Annu. Rev. Microbiol. , 1995; and Rosenfeid et al., Cell 68:143, 1992.

The choice of expression vector depends on the intended host cells in which the vector is to be expressed. Typically, the expression vectors contain a promoter and other regulatory ces (e.g., enhancers) that are operably linked to the poiynucleotides encoding a Factor P-binding antibody chain or fragment. In some embodiments, an inducible promoter is employed to prevent expression of ed ces except under inducing conditions. inducible promoters include, 9.9., arabinose, lacZ, metallothionein promoter or a heat shock promoter. Cultures of transformed organisms can be ed under ucing conditions without biasing the population for coding sequences whose expression products are better tolerated by the host cells. in addition to promoters, other regulatory elements may also be required or desired for efficient expression of a Factor ing antibody chain or nt.

These elements typically include an ATG initiation codon and adjacent ribosome binding site or other sequences. In addition, the efficiency of expression may be enhanced by the inclusion of enhancers appropriate to the cell system in use (see, e.g., Scharf et at, Results Probl. Cell Differ. 20:125, 1994; and Bittner et a/., Meth. Enzymoi., 153:516, 1987). For example, the SV4O enhancer or CMV er may be used to increase expression in mammalian host cells.

The expression vectors may also provide a secretion signal sequence position to form a fusion protein with ptides encoded by inserted Factor P-binding antibody sequences. More often, the ed Factor P—binding antibody sequences are linked to PCT/IBZOIZ/057394 1 08 a signal sequences before inclusion in the vector. Vectors to be used to receive sequences encoding Factor P-binding antibody light and heavy chain variable domains sometimes also encode constant regions or parts thereof. Such vectors allow expression of the variable regions as fusion proteins with the constant regions thereby leading to production of intact dies or fragments f. Typically, such constant regions are human.

The host cells for harboring and sing the Factor P-binding antibody chains can be either prokaryotic or eukaryotic. E. coli is one prokaryotic host useful for cloning and expressing the polynucleotides of the present invention. Other microbial hosts suitable for use include bacilli, such as Bacillus subtilis, and other enterobacteriaceae, such as Salmonella, Serratia, and various Pseudomonas s. In these prokaryotic hosts, one can also make expression vectors, which typically contain expression control sequences compatible with the host cell (6.9., an origin of replication). In addition, any number of a variety of well-known promoters will be present, such as the lactose promoter system, a tryptophan (trp) promoter system, a beta-lactamase promoter system, or a promoter system from phage lambda. The promoters typically control expression, ally with an operator ce, and have ribosome binding site sequences and the like, for initiating and completing transcription and translation. Other microbes, such as yeast, can also be employed to express Factor P—binding polypeptides of the invention. Insect cells in combination with baculovirus vectors can also be used.

In some preferred ments, ian host cells are used to express and produce the Factor P —binding ptides of the present invention. For e, they can be either a hybridoma cell line expressing endogenous globulin genes (e.g., the 1D6.C9 myeloma hybridoma clone as bed in the Examples) or a mammalian cell line harboring an exogenous expression vector (e.g., the SP2/O myeloma cells exemplified below). These include any normal mortal or normal or abnormal immortal animal or human cell. For example, a number of suitable host cell lines capable of secreting intact immunoglobulins have been developed including the CH0 cell lines, various Cos cell lines, HeLa cells, myeloma cell lines, transformed B-cells and omas. The use of mammalian tissue cell culture to express polypeptides is discussed lly in, e.g., ker, FROM GENES TO CLONES, VCH Publishers, N.Y., N.Y., 1987. Expression vectors for mammalian host cells can include sion control sequences, such as an origin of replication, a promoter, and an enhancer (see, 9.9., Queen, at at, lmmunol. Rev. 89:49-68, 1986), and necessary sing information sites, such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcriptional terminator sequences. These expression vectors usually PCT/[320121057394 1 09 contain promoters derived from mammalian genes or from mammalian viruses. Suitable promoters may be constitutive, cell type-specific, stage—specific, and/or modulatable or regulatable. Useful promoters include, but are not limited to, the metallothionein promoter, the constitutive adenovirus major late promoter, the dexamethasone-inducible MMTV promoter, the SV40 promoter, the MRP pollll promoter, the constitutive MPSV er, the tetracycline-inducible CMV promoter (such as the human immediate-early CMV promoter), the constitutive CMV promoter, and promoter-enhancer combinations known in the art.

Methods for introducing expression vectors containing the polynucleotide sequences of interest vary depending on the type of cellular host. For e, calcium chloride transfection is commonly utilized for prokaryotic cells, whereas calcium phosphate treatment or oporation may be used for other cellular hosts. (See generally Sambrook, etai., . Other methods e, e.g., electroporation, calcium phosphate treatment, liposome—mediated transformation, injection and microinjection, ballistic methods, mes, liposomes, polycationmucleic acid conjugates, naked DNA, artificial s, fusion to the herpes virus structural protein VP22 (Elliot and O'Hare, Cell 88:223, 1997), agent-enhanced uptake of DNA, and ex vivo transduction.

For long-term, high-yield production of recombinant ns, stable expression will often be desired. For e, cell lines which stably express Factor P ~binding dy chains or binding fragments can be prepared using expression vectors of the invention which contain viral origins of ation or endogenous expression elements and a selectable marker gene. Following the introduction of the vector, cells may be allowed to grow for 1—2 days in an enriched media before they are switched to ive media. The purpose of the selectable marker is to confer resistance to selection, and its presence allows growth of cells which successfully express the introduced sequences in selective media. Resistant, stably transfected cells can be proliferated using tissue culture techniques appropriate to the cell type.

Generation of monoclonal antibodies of the invention onal antibodies (mAbs) can be produced by a variety of techniques, including conventional monoclonal antibody methodology e.g., the rd somatic cell hybridization technique of Kohler and Milstein, 1975 Nature 256: 495. Many techniques for producing monoclonal antibody can be employed e.g., viral or oncogenic transformation of B lymphocytes.

ZOIZ/057394 1 10 An animal systems for preparing hybridomas include the murine, rat and rabbit systems. Hybridoma production in the mouse is a well established procedure.

Immunization protocols and techniques for isolation of immunized cytes for fusion are known in the art. Fusion partners (e.g., murine myeloma cells) and fusion procedures are also known.

Chimeric or humanized antibodies of the present ion can be prepared based on the sequence of a murine monoclonal antibody prepared as described above.

DNA encoding the heavy and light chain immunoglobulins can be obtained from the murine hybridoma of interest and engineered to contain rine (e.g.,. human) immunoglobulin sequences using standard molecular biology techniques. For example, to create a chimeric antibody, the murine variable regions can be linked to human constant regions using methods known in the art (see e.g., U.S. Patent No. 4,816,567 to Cabilly et al.). To create a humanized antibody, the murine CDR regions can be inserted into a human framework using s known in the art. See e.g., US. Patent No. 5225539 to Winter, and US. Patent Nos. 5530101; 5585089; 2 and 6180370 to Queen et al.

In a certain embodiment, the antibodies of the invention are human monoclonal antibodies. Such human monoclonal antibodies directed against Factor P can be generated using transgenic or transchromosomic mice carrying parts of the human immune system rather than the mouse system. These transgenic and transchromosomic mice include mice referred to herein as HuMAb mice and KM mice, respectively, and are collectively referred to herein as "human lg mice." The HuMAb mouse® (Medarex, lnc.) contains human immunoglobulin gene miniloci that encode un-rearranged human heavy (u and v) and K light chain immunoglobulin sequences, er with targeted mutations that inactivate the nous u and K chain loci (see e.g., g, et al., 1994 Nature 368(6474): 856~ 859). Accordingly, the mice exhibit reduced expression of mouse IgM or K, and in response to immunization, the introduced human heavy and light chain transgenes undergo class switching and somatic mutation to generate high affinity human lgGK onal (Lonberg, N. et al., 1994 supra; reviewed in Lonberg, N., 1994 ok of Experimental Pharmacology 113:49-101; Lonberg, N. and Huszar, D., 1995 . Rev. lmmunol.13: 65—93, and Harding, F. and Lonberg, N., 1995 Ann. N. Y. Acad. Sci. 764:536-546). The preparation and use of HuMAb mice, and the genomic modifications carried by such mice, is further bed in Taylor, L. et al., 1992 Nucleic Acids Research 20:6287—6295; Chen, J. et at., 1993 lnternational immunology 5: 647-656; PCT/IB2012J057394 1 11 Tuaillon et al., 1993 Proc. Natl. Acad. Sci. USA 0-3724; Choi et al., 1993 Nature Genetics 42117—123; Chen, J. eta/., 1993 EMBO J. 12: 821-830; Tuaillon et al., 1994 J. lmmunol. 152:2912-2920; Taylor, L. etal., 1994 international Immunology 1; and Fishwild, D. etal., 1996 Nature Biotechnology 14: 845-851, the contents of all of which are hereby specifically incorporated by reference in their entirety. See further, US.

Patent Nos. 5,545,806; 5,569,825; 126; 5,633,425; 5,789,650; 5,877,397; ,661,016; 5,814,318; 5,874,299; and 5,770,429; all to Lonberg and Kay; US. Patent No. 5,545,807 to Surani et at; PCT Publication Nos. WO 92103918, WO 93112227, WO 94/25585, WO 97113852, WO 98/24884 and WO 62, all to Lonberg and Kay; and PCT ation No. WO 01/14424 to Korman et a}. in another embodiment, human antibodies of the invention can be raised using a mouse that carries human immunoglobulin sequences on transgenes and transchomosomes such as a mouse that carries a human heavy chain transgene and a human light chain transchromosome. Such mice, referred to herein as “KM mice", are described in detail in PCT Publication WO 02/43478 to lshida et al.

Still further, alternative transgenic animal systems expressing human immunoglobulin genes are available in the art and can be used to raise Factor P -binding antibodies of the invention. For example, an ative transgenic system referred to as the Xenomouse (Abgenix, inc.) can be used. Such mice are bed in, 6.9., US.

Patent Nos. 5,939,598; 6,075,181; 6,114,598; 6, 150,584 and 6,162,963 to Kucherlapati etal.

Moreover, alternative transchromosomic animal systems sing human immunoglobulin genes are available in the art and can be used to raise Factor P ng antibodies of the invention. For example, mice ng both a human heavy chain transchromosome and a human light chain romosome, referred to as “TC mice" can be used; such mice are described in Tomizuka et al., 2000 Proc. Natl. Acad. Sci.

USA 97:722-727. Furthermore, cows carrying human heavy and light chain transchromosomes have been bed in the art (Kuroiwa et al., 2002 Nature Biotechnology 20:889-894) and can be used to raise Factor P -binding antibodies of the invention.

Human monoclonal antibodies of the invention can also be prepared using phage display methods for screening libraries of human immunoglobulin genes. Such phage display methods for isolating human antibodies are established in the art or described in the examples below. See for example: US. Patent Nos. 5,223,409; 5,403,484; and PCT/IBZOIZ/057394 1 12 ,571,698 to Ladner et al.; US. Patent Nos. 5,427,908 and 5,580,717 to Dower et at; US. Patent Nos. 5,969,108 and 6,172,197 to McCafferty et al.; and US. Patent Nos. ,885,793; 6,521,404; 6,544,731; 6,555,313; 6,582,915 and 081 to Griffiths et al.

Human monoclonal dies of the invention can also be prepared using SCID mice into which human immune cells have been reconstituted such that a human antibody response can be generated upon immunization. Such mice are described in, for example, US. Patent Nos. 996 and 5,698,767 to Wilson at al.

Framework or F0 engineering Engineered antibodies of the invention include those in which modifications have been made to framework residues within VH and/or VL, e.g. to e the properties of the antibody. Typically such framework modifications are made to decrease the immunogenicity of the antibody. For example, one approach is to "backmutate" one or more framework residues to the corresponding germline sequence. More cally, an antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the dy framework ces to the ne sequences from which the antibody is derived. To return the framework region sequences to their germline configuration, the somatic ons can be "backmutated" to the germline ce by, for example, site-directed mutagenesis. Such "backmutated" antibodies are also intended to be encompassed by the invention.

Another type of ork modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell — es to thereby reduce the potential immunogenicity of the antibody. This approach is also referred to as "deimmunization" and is described in further detail in US. Patent ation No. 20030153043 by Carr 91‘ al.

In addition or alternative to modifications made within the framework or CDR regions, antibodies of the invention may be engineered to include modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, ment fixation, Fc receptor binding, and/or antigen-dependent cellular cytotoxicity. Furthermore, an antibody of the invention may be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or be modified to alter its glycosylation, again to alter one or more functional properties of the dy. Each of these embodiments is described in further detail below. The numbering of residues in the Fc region is that of the EU index of Kabat.

PCT/IBZOIZ/057394 1 13 In one embodiment, the hinge region of CH1 is modified such that the number of cysteine residues in the hinge region is d, e.g., sed or decreased. This approach is described r in US. Patent No. 5,677,425 by Bodmer et al. The number of ne residues in the hinge region of CH1 is d to, for example, tate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.

In another embodiment, the Fc hinge region of an antibody is mutated to decrease the biological half—life of the antibody. More specifically, one or more amino acid mutations are introduced into the CH2-CH3 domain interface region of the Fc-hinge fragment such that the dy has impaired Staphylococcyl protein A (SpA) binding relative to native Fc-hinge domain SpA binding. This approach is described in further detail in US. Patent No. 6,165,745 by Ward et al.

In another embodiment, the antibody is modified to increase its biological half-life.

Various approaches are possible. For example, one or more of the following mutations can be uced: T252L, T2548, T256F, as described in U.S. Patent No. 6,277,375 to Ward. Alternatively, to increase the biological half life, the antibody can be altered within the CH1 or CL region to contain a salvage receptor binding epitope taken from two loops ofa CH2 domain of an Fc region of an lgG, as described in US. Patent Nos. 5,869,046 and 6,121,022 by Presta etal. in yet other embodiments, the Fc region is altered by replacing at least one amino acid residue with a different amino acid residue to alter the effector functions of the antibody. For example, one or more amino acids can be replaced with a different amino acid residue such that the antibody has an altered affinity for an effector ligand but s the antigen-binding ability of the parent antibody. The effector ligand to which affinity is altered can be, for example, an F0 or or the C1 component of complement. This ch is described in further detail in U.S. Patent Nos. 5,624,821 and 5,648,260, both by Winter et al.

In another embodiment, one or more amino acids selected from amino acid residues can be replaced with a different amino acid residue such that the antibody has altered C1q binding and/or reduced or abolished complement dependent cytotoxicity (CDC). This approach is bed in further detail in US. Patent Nos. 551 by ldusogie et a].

PCT/[32012/057394 1 14 In another embodiment, one or more amino acid residues are altered to thereby alter the ability of the antibody to fix ment. This approach is described further in PCT Publication WO 94/29351 by Bodmer et al. in yet another embodiment, the Fc region is modified to increase the ability of the dy to mediate antibody dependent cellular cytotoxicity (ADCC) and/or to increase the affinity of the antibody for an Fcy receptor by modifying one or more amino acids.

This ch is described further in PCT ation WO 00/42072 by .

Moreover, the binding sites on human igG1 for Fcle, FcyRiI, chRlll and FcRn have been mapped and variants with improved binding have been described (see Shields, R.L. eta/., 2001 J. Biol. Chen. 276:6591~6604). in still another embodiment, the glycosylation of an dy is modified. For example, an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation).

Glycosylation can be altered to, for example, increase the affinity of the antibody for "antigen'. Such carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence. For example, one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to y eliminate glycosylation at that site. Such aglycosylation may increase the affinity of the antibody for antigen. Such an approach is described in r detail in US. Patent Nos. 5,714,350 and 6,350,861 by Co et al.

Additionally or alternatively, an antibody can be made that has an altered type of glycosylation, such as a hypofucosylated antibody having reduced amounts of fucosyl residues or an antibody having increased bisecting GIcNac structures. Such altered glycosylation patterns have been demonstrated to increase the ADCC ability of dies. Such carbohydrate modifications can be accomplished by, for e, expressing the antibody in a host cell with d glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells in which to express recombinant antibodies of the invention to thereby produce an dy with altered glycosylation. For example, EP 1,176,195 by Hang eta]. bes a cell line with a functionally disrupted FUT8 gene, which encodes a fucosyl transferase, such that antibodies expressed in such a cell line exhibit hypofucosylation. PCT Publication WO 03/035835 by Presta describes a variant CHO cell line, Lecl3 cells, with reduced ability to attach fucose to Asn(297)—linked carbohydrates, also resulting in hypofucosylation of dies expressed in that host cell (see also Shields, R.L. et al., 2002 J. Biol. Chem. 277:26733—26740). PCT Publication WO 99/54342 by Umana et al. ’l 15 describes cell lines engineered to express rotein-modifying glycosyl transferases (e.g., beta(1,4)—N acetylglucosaminyltransferase lll (GnTlll)) such that antibodies expressed in the engineered cell lines t increased bisecting GlcNac structures which results in increased ADCC activity of the antibodies (see also Umana et al., 1999 Nat. Biotech. 17:176-180).

Methods of ering Altered dies As discussed above, the Factor P -binding antibodies having VH and VL sequences or full length heavy and light chain ces shown herein can be used to create new Factor P—binding antibodies by modifying full length heavy chain and/or light chain sequences, VH and/or VL sequences, or the nt region(s) attached thereto.

Thus, in another aspect of the invention, the structural features of a Factor P-binding antibody of the invention are used to create structurally related Factor P-binding antibodies that retain at least one functional property of the antibodies of the ion, such as binding to human Factor P and also inhibiting one or more functional properties of Factor P (6.9., inhibiting MAC deposition in a MAC deposition assay, inhibit red blood cell lysis in a hemolytic assay).

For example, one or more CDR regions of the dies of the present invention, or mutations thereof, can be ed recombinantly with known framework regions and/or other CDRs to create additional, recombinantly-engineered, Factor P — binding antibodies of the invention, as discussed above. Other types of modifications include those described in the previous section. The starting material for the engineering method is one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. To create the engineered antibody, it is not necessary to actually prepare (i.e., express as a protein) an antibody having one or more of the VH and/or VL sequences provided herein, or one or more CDR regions thereof. Rather, the information contained in the ce(s) is used as the starting material to create a "second tion" sequence(s) derived from the original sequence(s) and then the "second generation" sequence(s) is prepared and expressed as a protein.

Accordingly, in another embodiment, the invention provides a method for preparing a Factor P-binding antibody consisting of a heavy chain le region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID NOs: 1, 15, 29, 43, 57, 71, 85, 99, 113, 127, 141, 155, 169, 183, 197, 211, 225, 239, 253, and 267, a CDR2 sequence ed from the group consisting of SEQ ID NOS: 2, 16, 30, 44, 58, 72, 86, 100, 114, 128, 142, 156, 170, 184, 198, 212, 226, 240, 254, and 1 16 268, and/or a CDR3 sequence ed from the group ting of SEQ ID NOs: 3, 17, 31, 45, 59, 73, 87, 101, 115, 129, 143, 157, 171, 185, 199, 213, 227, 241, 255, and 269; and a light chain variable region antibody sequence having a CDR1 sequence ed from the group consisting of SEQ ID NOs: 4, 18, 32, 46, 60, 74, 88, 102, 116, 130, 144, 158, 172, 186, 200, 214, 228, 242, 256, and 270, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 5, 19, 33, 47, 61, 75, 89, 103, 117, 131, 145, 159, 173, 187, 201, 215, 229, 243, 257, and 271, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 6, 20, 34, 48, 62, 76, 90, 104, 118, 132, 146, 160, 174, 188, 202, 216, 230, 244, 258, and 272; altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein.

Accordingly, in another embodiment, the invention provides a method for preparing a Factor ing dy consisting of a heavy chain le region antibody sequence having a CDR1 sequence selected from the group ting of SEQ ID NOs: 281, 287, 293, 299, 305, 311, 317, 323, 329, 335, 341, 347, 353, 359, 365, 371, 377, 383, 389, and 395, a CDR2 sequence selected from the group consisting of SEQ ID NOs: 282, 288, 294, 300, 306, 312, 318, 324, 330, 336, 342, 348, 354, 360, 366, 372, 378, 384, 390, and 396, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 283, 289, 295, 301, 307, 313, 319, 325, 331, 337, 343, 349, 355, 361, 367, 373, 379, 385, 391, and 397; and a light chain variable region antibody sequence having a CDR1 sequence selected from the group consisting of SEQ ID N08: 284, 290, 296, 302, 308, 314, 320, 326, 332, 338, 344, 350, 356, 362, 368, 374, 380, 386, 392, and 398, a CDR2 sequence selected from the group consisting of SEQ ID N05: 285, 291, 297, 303, 309, 315, 321, 327, 333, 339, 345, 351, 357, 363, 369, 375, 381, 387, 393, and 399, and/or a CDR3 sequence selected from the group consisting of SEQ ID NOs: 286, 292, 298, 304, 310, 316, 322, 328, 334, 340, 346, 352, 358, 364, 370, 376, 382, 388, 394, and 400; altering at least one amino acid residue within the heavy chain variable region antibody sequence and/or the light chain variable region antibody sequence to create at least one altered antibody sequence; and expressing the d antibody sequence as a protein.

Accordingly, in another embodiment, the invention provides a method for preparing a Factor P—binding antibody optimized for expression in a mammalian cell consisting of: a full length heavy chain antibody ce having a sequence selected from the group of SEQ ID NOs: 9, 23, 37, 51, 65, 79, 93, 107, 121, 135, 149, 163, 177, 191, 205, 219, 233, 247, 261 and 275; and a full length light chain dy sequence WO 93762 1 17 having a sequence ed from the group of SEQ lD NOs: 10, 24, 38, 52, 66, 80, 94, 108, 122, 136, 150, 164, 178, 192, 206, 220, 234, 248, 262, and 276; altering at least one amino acid residue within the full length heavy chain antibody ce and/or the full length light chain antibody sequence to create at least one altered antibody sequence; and expressing the altered antibody sequence as a protein. In one embodiment, the alteration of the heavy or light chain is in the framework region of the heavy or light chain.

The altered antibody sequence can also be prepared by screening antibody libraries having fixed CDR3 sequences or minimal essential binding determinants as bed in US20050255552 and diversity on CDR1 and CDR2 sequences. The screening can be performed according to any ing technology appropriate for screening antibodies from antibody libraries, such as phage display technology.

Standard molecular biology techniques can be used to prepare and express the altered antibody sequence. The antibody encoded by the altered antibody sequence(s) is one that retains one, some or all of the onal properties of the Factor P-binding antibodies described herein, which functional properties include, but are not limited to, specifically binding to human and/or cynomolgus Factor P; and the antibody inhibit red blood cell lysis in a hemolytic assay.

In certain embodiments of the methods of engineering antibodies of the invention, mutations can be introduced randomly or ively along all or part of an Factor P— binding antibody coding sequence and the resulting modified Factor P-binding dies can be screened for binding activity and/or other functional ties as described herein. Mutational methods have been described in the art. For example, PCT Publication WO 02/092780 by Short bes methods for creating and ing antibody mutations using saturation mutagenesis, tic ligation assembly, or a combination thereof. Alternatively, PCT Publication WO 679 by Lazar et al. describes methods of using computational screening methods to optimize physiochemical properties of antibodies. 1 18 In certain embodiments of the invention antibodies have been engineered to remove sites of deamidation. Deamidation is known to cause structural and functional changes in a peptide or protein. Deamindation can result in decreased bioactivity, as well as alterations in pharmacokinetics and antigenicity of the protein pharmaceutical.

(Anal Chem. 2005 Mar 1:77(5):1432-9).

The functional properties of the d antibodies can be assessed using standard assays available in the art and/or described herein, such as those set forth in the Examples (e.g., ELlSAs).

Prophylactic and eutic Uses Antibodies that binds Factor P as bed herein, can be used at a therapeutically useful concentration for the treatment of a disease or disorder associated with increased complement activity by administering to a subject in need thereof an effective amount of the antibodies or antigen binding fragments of the invention. In a specific embodiment, the t invention provides a method of treating age—related macular degeneration (AMD) by administering to a subject in need f an effective amount of the antibodies of the invention.

The dies of the invention can be used, inter alia, to prevent progression of dry AMD to wet AMD, to slow and/or t progression of phic y, to treat or prevent macular edema, to reduce the frequency of Lucentis ion and to improve vision lost due to dry and wet AMD progression. it can also be used in combination with anti—VEGF therapies for the ent of wet AMD patients.

Treatment and/or prevention of r disease such as AMD can be determined by an ophthalmologist or health care professional using clinically relevant measurements of visual function and/or retinal anatomy. Treatment of AMD means any action (e.g., administration of an anti—Factor P antibody described herein) contemplated to improve or preserve visual function and/or retinal anatomy. in addition, prevention as it relates to AMD means any action (e.g., administration of an anti-Factor P antibody described herein) that prevents or slows a ing in visual function, retinal anatomy, and/or an AMD disease parameter, as defined herein, in a patient at risk for said worsening.

Visual function may include, for example, visual acuity, visual acuity with low illumination, visual field, central visual field, peripheral vision, contrast sensitivity, dark adaptation, photostress recovery, color discrimination, reading speed, dependence on assistive devices (e.g., large typeface, magnifying devices, telescopes), facial 1 19 ition, proficiency at operating a motor vehicle, ability to perform one or more activities of daily , and/or patient—reported satisfaction related to visual function.

THus, treatment of AMD can be said to occur where a subject has an at least 10% decrease or lack of a 10% or more se in time to a pre-specified degree of dark adaptation. in addition, treatment of AMD can be said to occur where a subject exhibits at least a 10% reduction or lack of a 10% or more se in total area of central visual scotoma expressed as a visual angle determined by a qualified health care professional (i.e., opthalmologist).

Exemplary measures of visual function include Snellen visual acuity, ETDRS visual acuity, low—luminance visual acuity, Amsler grid, Goldmann visual field, Humphrey visual field, microperimetry, Pelli-Robson charts, SKILL card, lshihara color plates, Farnsworth D15 or D100 color test, and validated tests for reading speed, facial recognition, g simulations, and patient reported satisfaction. Thus, ent of AMD can be said to be achieved upon a gain of or failure to lose 2 or more lines (or 10 letters) of vision on an ETDRS scale. In on, treatment of AMD can be said to occur where a subject exhibits at least a 10% an increase or lack of 10% decrease in reading speed (words per minute). in addition, treamtne of AMD can be said to occur where a subject exhibits at least a 20% increase or lack of a 20% decrease in the proportion of correctly identified plates on an lshihara test or sequenced disks ona Farnsworth test.

Undesirable aspects of retinal anatomy that may be treated or prevented include, for example, drusen, soft drusen, hard , cuticular drusen, basal laminar drusen, confluent drusen, large drusen (e.g., greater than 125 s in er), RPE atrophy, eceptor atrophy, geographic atrophy, choroidal neovascularization, inal neovascularization, retinal neovascularization, classic choroidal neovascularization, occult choroidal neovascularization, retinal atous proliferation, chorioretinal anastomosis, an abnormality of choroidal anatomy, subretinal hemorrhage, intraretinal hemorrhage, vitreous hemorrhage, macular scar, subretinal fibrosis, and retinal fibrosis. Thus, treatment of, for example, geographic atrophy can be determined by a 20% or more reduction in lesion growth rate as compared to control or previously documented growth rate in the same subject in the same eye.

Exemplary means of assessing retinal anatomy include funduscopy, fundus photography, fluorescein angiography, indocyanine green angiography, ocular coherence tomography (OCT), spectral domain ocular coherence tomography, scanning laser lmoscopy, confocal microscopy, adaptive optics, fundus orescence, biopsy, sy, and immunohistochemistry. Thus, AMD can be said to be treated in a PCT/IBZOlZ/057394 ’l 20 subject upon a 10% reduction in the measurement of macular thickness as determined by OCT, and/or a reduction of hyperfluorescence as determined by cein angiography.

Exemplary measures of retinal y include drusen area, drusen volume, geographic atrophy lesion area, geographic atrophy growth rate, and cular membrane area.

In some embodiments, the present invention provides methods of treating a complement related disease or er by administering to a t in need thereof an ive amount of the antibodies of the invention. Examples of known complement related diseases or disorders include: neurological ers, multiple sclerosis, , Guillain Barre Syndrome, traumatic brain injury, Parkinson's disease, disorders of inappropriate or undesirable ment activation, alysis complications, hyperacute allograft rejection, xenograft rejection, interleukin-2 induced toxicity during lL— 2 y, inflammatory disorders, inflammation of autoimmune diseases, Crohn's disease, adult respiratory distress syndrome, thermal injury including burns or frostbite, post-ischemic reperfusion ions, myocardial infarction, balloon angioplasty, post- pump syndrome in cardiopulmonary bypass or renal bypass, hemodialysis, renal ischemia, mesenteric artery reperfusion after acrotic reconstruction, infectious disease or sepsis, immune complex disorders and autoimmune diseases, rheumatoid tis, systemic lupus erythematosus (SLE), SLE nephritis, proliferative nephritis, hemolytic anemia, and myasthenia . in addition, other known complement related disease are lung disease and ers such as dyspnea, hemoptysis, ARDS, asthma, c obstructive pulmonary disease (COPD), ema, pulmonary embolisms and infarcts, pneumonia, fibrogenic dust diseases, inert dusts and minerals (e.g., silicon, coal dust, beryllium, and asbestos), pulmonary fibrosis, organic dust diseases, chemical injury (due to irritant gasses and chemicals, e.g., chlorine, phosgene, sulfur dioxide, hydrogen sulfide, nitrogen dioxide, ammonia, and hydrochloric acid), smoke injury, thermal injury (e.g., burn, freeze), asthma, allergy, bronchoconstriction, hypersensitivity pneumonitis, parasitic diseases, Goodpasture’s Syndrome, pulmonary vasculitis, and immune complex-associated inflammation.

In a specific embodiment, the present invention provides methods of treating a complement related disease or er by stering to a subject in need thereof an effective amount of the antibodies of the invention, wherein said disease or disorder is asthma, arthritis (e.g., toid arthritis), autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, ischemia-reperfusion injuries, Barraquer—Simons 1 21 Syndrome, hemodialysis, systemic lupus, lupus erythematosus, psoriasis, multiple sclerosis, transplantation, diseases of the central s system such as Alzheimer's disease and other egenerative conditions, aHUS, glomerulonephritis, bullous pemphigoid or MPGN II.

In a specific embodiment, the present invention provides methods of treating glomerulonephritis by stering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention. Symptoms of glomerulonephritis include, but not limited to, proteinuria; reduced glomerular filtration rate (GFR); serum electrolyte changes including azotemia (uremia, excessive blood urea en--BUN) and salt retention, leading to water retention ing in hypertension and edema; ria and abnormal urinary sediments including red cell casts; hypoalbuminemia; hyperlipidemia; and lipiduria. in a specific embodiment, the present invention provides methods of treating paroxysmal nocturnal hemoglobinuria (PNH) by administering to a t in need f an effective amount of a composition comprising an antibody of the present invention.

In a specific embodiment, the t invention es methods of reducing the dysfunction of the immune and hemostatic systems associated with extracorporeal circulation by administering to a subject in need thereof an effective amount of a composition comprising an antibody of the present invention. The dies of the present invention can be used in any procedure which involves ating the patient’s blood from a blood vessel of the patient, through a conduit, and back to a blood vessel of the patient, the conduit having a luminal surface comprising a material capable of causing at least one of complement tion, platelet activation, leukocyte activation, or platelet-leukocyte adhesion. Such procedures e, but are not d to, all forms of ECG, as well as procedures involving the introduction of an artificial or foreign organ, tissue, or vessel into the blood circuit of a patient.

Subjects to be treated with therapeutic agents of the present ion can also be administered other therapeutic agents with know methods of treating conditions associated with macular ration, such as antibiotic treatments as described in US Pat. No. 6,218,368. In other treatments, immunosuppressive agents such as cyclosporine, are agents capable of suppressing immune responses. These agents include cytotoxic drugs, corticosteriods, nonsteroidal nflammatory drugs (NSAIDs), specific T—lymphocyte immunosuppressants, and antibodies or fragments thereof (see Physicians' Desk Reference, 53rd edition, Medical Economics Company lnc., Montvale, N.J. (1999). Immunosuppressive treatment is typically continued at intervals for a period 012/057394 1 22 of a week, a month, three months, six months or a year. In some patients, ent is administered for up to the rest of a patient's life.

When the therapeutic agents of the present invention are administered together with another agent, the two can be stered tially in either order or simultaneously. In some aspects, an antibody of the present invention is administered to a subject who is also receiving therapy with a second agent (e.g., verteporfin). In other aspects, the binding molecule is administered in conjunction with surgical treatments.

Suitable agents for combination treatment with Factor P binding antibodies include agents known in the art that are able to modulate the activities of complement components (see, e.g., US. Pat. No. 5,808,109). Other agents have been reported to diminish complement-mediated activity. Such agents include: amino acids (Takada, Y. etal. logy 1978, 34, 509); phosphonate esters (Becker, L. Biochem. Biophy. Acta 1967, 147, 289); polyanionic substances (Conrow, R. B. etal. J. Med. Chem. 1980, 23, 242); sulfonyl des h, C.; Yoshimoto, M. J. Med. Chem. 1974, 17, 1160, and references cited therein); polynucleotides rcq, P. F. eta]. Biochem. Biophys. Res.

Commun. 1975, 67, 255); pimaric acids (Glovsky, M. M. etal. J. Immunol. 1969, 102, 1); porphines (Lapidus, M. and Tomasco, J. Immunopharmacol. 1981, 3, 137); several antiinflammatories (Burge, J. J. etal. J. Immunol. 1978, 120, 1625); phenols (Muller— Eberhard, H. J. 1978, in lar Basis of Biological Degradative Processes, Berlin, R.

D. el‘ al., eds. ic Press, New York, p. 65); and idines (Vogt, W. eta/ Immunology 1979, 36, 138). Some of these agents function by general inhibition of proteases and esterases. Others are not specific to any particular intermediate step in the complement pathway, but, rather, inhibit more than one step of complement activation. Examples of the latter compounds include the benzamidines, which block C1, C4 and 03b utilization (see, e.g., Vogt et al. Immunol. 1979, 36, 138).

Additional agents known in the art that can t activity of complement components include K-76, a fungal metabolite from Stachybotrys (Corey et al., J. Amer.

Chem. Soc. 104: 5551, 1982). Both K-76 and K~76 COOH have been shown to inhibit complement mainly at the C3b step (Hong et al., J. Immunol. 122: 2418, 1979; Miyazaki et al., iol. l. 24: 1091, 1980), and to prevent the generation ofa chemotactic factor from normal human complement (Bumpers et al., Lab. Clinc. Med. 102: 421, 1983). At high concentrations of K-76 or K—76 COOH, some inhibition of the reactions of 02, C3, C6, CT, and C9 with their respective preceding intermediaries is exhibited. K—76 or K—76 COOH has also been reported to inhibit the 03b inactivator system of complement (Hong et al., J. Immunol. 127: 104—108, 1981). Other suitable 1 23 agents for practicing methods of the present invention include griseofulvin (Weinberg, in Principles of Medicinal Chemistry, 2d Ed., Foye, W. 0., ed., Lea & r, Philadelphia, Pa., p. 813, 1981), isopannarin (Djura etal., Aust. J. Chem.36: 1057, 1983), and metabolites of Siphonodictyon coralli-phagum (Sullivan et al., Tetrahedron 37: 979, 1981) A combination therapy regimen may be additive, or it may produce synergistic results (e.g., reductions in complement pathway activity more than ed for the combined use of the two agents). in some embodiments, the present ion provide a combination therapy for ting and/or treating AMD or another complement related disease as described above with a Factor P binding antibody of the ion and an anti-angiogenic, such as anti-VEGF agent, or r anti-complement antibody such as an antibody or antigen binding fragment thereof that binds to complement factor 5 (C5). ation of anti-complement antibodies in one aspect, the invention provides combinations of any one or more of the anti- Factor P with an additional antibody that binds to and ts the activity of a different ent of the complement pathway. In particular, the invention includes any one or more of the anti-Factor P antibodies or antigen binding fragments described herein in combination with an antibody or antigen binding fragment that binds complement component 5 (CS). Examples of antibodies or antigen binding fragments thereof that bind to 05 and inhibit complement activation can be found, for example in US. Patent 8,241,628 (incorporated herein by reference). More specifically, antibodies or antigen binding fragments thereof that bind to C5 and inhibit the complement pathway are shown and described in Table 2. in one aspect the invention includes a combination of an anti- Factor P antibody or antigen binding fragment thereof as shown and described in Table 1 with the anti-C5 antibody 8109 from Table 2. More specifically, one aspect of the invention relates to a ation of antibody NV8962 from Table 1 (or an antigen binding fragment thereof) with antibody 8109 from Table 2 (or an antigen binding fragment thereof).

In one aspect the ations of anti-Factor P and anti-C5 antibodies described herein demonstrate a syntergistic inhibition of the complement pathway, particularly the alternative complement y. Such inhibition can be demonstrated, for example, using the tic or poly-IC assays described in the Examples below. Synergy in the inhibition of the alternative complement pathway, achieved using a combination of the actor—P and anti—C5 antibodies described herein can be ined using methods 1 24 that are well known in the art. For example, a synergistic effect of the combination of anti-Factor P antibody and anti-05 antibody can be determine relative to a merely additive effect using specific software, such as a Chalice Analyzer.

Briefly, Chalice Analyzer (Lehar et al, Nature Biotechnology 2009, 7:659) software can be used to determine whether the combination of complement inhibiting antibodies (e.g., anti-Factor P and anti—C5) acted synergistically to block complement activation.

Combination effects can be characterized by comparing each data point’s inhibition to that of a combination reference model that was derived from the single agent curves , Bravo, Parsons . The search for synergy: a al review from a response surface perspective. Pharmacol Rev 47(2): 331—85). in the Loewe additivity model (Loewe . Die tativen Prob/eme der Pharmako/ogie. Ergebn.

Physiol. 27: 47-187), [Loewe(Cx,C\/) is the inhibition that satisfies (CXIICX) + (Cy/Icy) = 1, and ICXIY are the effective concentrations at ILoewe for the fitted single agent curves. Loewe vity is the generally ed reference for synergy (Greco et al.), as it represents the combination response generated if X and Y are the same compound.

Potency shifting is usually shown using an isobologram (Greco et al.) which shows how much less drug is ed in combination to achieve a desired effect level, when compared to the single agent doses needed to reach that effect. The choice of effect level for the isobologram display and combination index calculations can either be ly or tically selected in the Chalice Analyzer. The automatic iso—level ion algorithm finds the observed [data with the the largest lam—(Loewe, excluding those points with Idata exceeding the lesser single agent’s Imax. This exclusion is applied to ensure that the isobologram reflects the best synergy at levels covered by both single agents. Having selected an isobologram level ’cut, the isobologram is drawn by identifying the locus of trations that correspond to crossing the chosen iso~ level. The isobologram shows the rd isobolographic analysis of synergy compared to the Loewe dose-additive “drug-with—itself’ standard. For a specified isobologram level, the ed iso-effect contour (e.g., curved line in Figure 3) is displayed with the tical dose-additive contour (e.g., straight line in Figure 3), on an lCeffect-normalized linear concentration scale for both substances in the combination. The Dose-additive reference is always a line connecting the two lCefiect concentrations. The lCefiect crossing points are found by interpolating the fitted sigmoidal dose response curves.

Potency shifting is scored as the combination index (Chou, Talalay (1984).

Quantitative analysis of dose-effect onships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul 22: 27-55) Cl. For a chosen iso—effect level Icut, Cl. = (Ox/ECX). + (CY/ECY)1, where (OX/ECX). for a particular data point is the ratio of the X compound’s measured concentration to its effective concentration at the chosen tion level. The Cl can be thought of as a rough estimate of how much drug was needed in combination relative to the single agent doses ed to achieve the chosen effect level, and a value of 0.1 means that only a tenth of equivalent amounts of the single agents were needed for the combination to reach the same effect level. Cl values in the range of 0.5-0.7 are typical for in vitro measurements of current clinical combinations (Greco et al.). A CI value of 1.0 is indicative of an additive effect of a combination of antibodies, while a Cl value of less than 0.5 is indiciative of a strong synergistic effect ing from the antibody combination. in the Chalice Analyzer, the best Cl is reported from the many combination index values calculated for each [cut crossing concentration. Among all the measured CI values, the one with the t signal-to-noise level is reported as the best combination index.

Combinations of anti-Factor P and anti-C5 antibodies as described herein can be administered singly or as a single composition. In addition, the relative dose of an anti- Factor P and anti-CS dy can be in a ratio of 1:1, or may be in a different ratio. The specific dose of an anti-Factor P antibody relative to an 5 antibody may ultimately be determined by a treating physician or health care professional to achieve improvement in the pathological ion being treated. For example, when a combination as described herein is used to treat AMD, a physician or health care professional may taylor the relative doses of the actor P and 5 antibodies so as to achive optimal therapeutic benefit as determined using the measurements and criteria described herein.

Pharmaceutical Compositions The invention provides pharmaceutical compositions comprising the Factor P— binding antibodies (intact or binding fragments) formulated together with a pharmaceutically acceptable carrier. The compositions can additionally n one or more other therapeutic agents that are suitable for treating or preventing, for e, pathological angiogeneis or tumor growth. Pharmaceutically acceptable carriers e or stabilize the composition, or can be used to facilitate ation of the composition. Pharmaceutically acceptable carriers e solvents, dispersion media, 1 26 coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like that are physiologically compatible.

A ceutical composition of the present ion can be administered by a variety of methods known in the art. The route and/or mode of administration vary depending upon the desired results. it is preferred that administration be intravitreal, intravenous, intramuscular, intraperitoneal, or subcutaneous, or administered proximal to the site of the . The pharmaceutically acceptable carrier should be suitable for intravitreal, intravenous, intramuscular, subcutaneous, eral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active compound, i.e., antibody, bispecific and multispecific molecule, may be coated in a material to protect the compound from the action of acids and other natural conditions that may inactivate the compound.

The ition should be e and fluid. Proper fluidity can be maintained, for e, by use of coating such as lecithin, by maintenance of required particle size in the case of dispersion and by use of surfactants. in many cases, it is preferable to include isotonic agents, for e, sugars, polyalcohols such as mannitol or sorbitol, and sodium chloride in the composition. Long—term absorption of the injectable compositions can be brought about by including in the composition an agent which delays tion, for example, aluminum monostearate or gelatin.

Pharmaceutical compositions of the invention can be prepared in accordance with methods well known and routinely practiced in the art. See, e.g., Remington: The Science and Practice of Pharmacy, Mack hing Co., 20th ed., 2000; and Sustained and Controlled Release Drug Delivery Systems, J.R. Robinson, ed., Marcel Dekker, Inc, New York, 1978. ceutical compositions are preferably manufactured under GMP ions. Typically, a therapeutically effective dose or efficacious dose of the Factor P- binding antibody is employed in the pharmaceutical compositions of the invention. The Factor P—binding antibodies are formulated into pharmaceutically acceptable dosage forms by tional methods known to those of skill in the art. Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response). For example, a single bolus may be administered, l divided doses may be administered over time or the dose may be proportionally reduced or sed as indicated by the exigencies of the therapeutic situation. It is especially advantageous to formulate parenteral compositions in dosage unit form for ease of administration and mity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the ts to be treated; each unit contains a 1 27 predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required ceutical carrier.

Actual dosage levels of the active ingredients in the pharmaceutical compositions of the present invention can be varied so as to obtain an amount of the active ient which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, t being toxic to the patient. The selected dosage level depends upon a variety of pharmacokinetic factors including the activity of the particular compositions of the present invention ed, or the ester, salt or amide thereof, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, the duration of the treatment, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, ion, general health and prior medical history of the patient being treated, and like factors.

A physician or veterinarian can start doses of the antibodies of the ion employed in the ceutical composition at levels lower than that required to achieve the desired therapeutic effect and gradually increase the dosage until the desired effect is achieved. In general, ive doses of the compositions of the present invention, for the treatment of an allergic inflammatory disorder described herein vary depending upon many ent factors, including means of administration, target site, physiological state of the patient, r the patient is human or an , other medications administered, and whether treatment is prophylactic or therapeutic. Treatment dosages need to be titrated to optimize safety and efficacy. For systemic administration with an antibody, the dosage ranges from about 0.0001 to 100 mg/kg, and more usually 0.01 to mg/kg, of the host body . For intravitreal administration with an antibody, the dosage may range from 0.1 mg/eye to 5mg/eye. For example, 0.1 mg/ml, 0.2 mg/ml, 0.3 mg/ml, 0.4 mg/ml, 0.5 mg/ml, 0.6 mg/ml, 0.7 mg/ml, 0.8 mg/ml, 0.9 mg/ml, 1.0 mg/ml, 1.1 mg/ml, 1.2 mg/ml, 1.3 mg/ml, 1.4 mg/ml, 1.5 mg/ml, 1.6 mg/ml, 1.7 mg/ml, 1.8 mg/ml, 1.9 mg/ml, 2.0 mg/ml, 2.1 mg/ml, 2.2 mg/ml, 2.3 mg/ml, 2.4 mg/ml, 2.5 mg/ml, 2.6 mg/ml, 2.7 mg/ml, 2.8 mg/ml, 2.9 mg/ml, 3.0 mg/ml, 3.1 mg/ml, 3.2 mg/ml, 3.3 mg/ml, 3.4 mg/ml, 3.5 mg/ml, 3.6 mg/ml, 3.7 mg/ml, 3.8 mg/ml, 3.9 mg/ml, 4.0 mg/ml, 4.1 mg/ml, 4.2 mg/ml, 4.3 mg/ml, 4.4 mg/ml, 4.5 mg/ml, 4.6 mg/ml, 4.7 mg/ml, 4.8 mg/ml, 4.9 mg/ml, or 5.0 mg/ml.

An exemplary treatment regime entails systemic stration once per every two weeks or once a month or once every 3 to 6 months. An exemplary treatment regime entails systemic administration once per every two weeks or once a month or once every 3 to 6 months PCT/IBZOI2/057394 1 28 dy is usually administered on multiple occasions. Intervals between single dosages can be weekly, monthly or yearly. intervals can also be irregular as indicated by measuring blood levels of Factor P-binding antibody in the patient. in addition alternative dosing intervals can be determined by a physician and stered monthly or as necessary to be cious. Efficacy is based on lesion growth, rate of is rescue, retinal thinckness as determined by Spectral Domain-optical Optical nce Tomography (SD-OCT), and secondary visual acuity. In some methods of systemic administration, dosage is adjusted to achieve a plasma antibody concentration of 1—1000 pg/ml and in some methods 25—500 ug/ml. Alternatively, antibody can be administered as a sustained release formulation, in which case less frequent administration is required. Dosage and frequency vary depending on the half—life of the antibody in the patient. in l, humanized antibodies show longer half life than that of chimeric antibodies and nonhuman antibodies. The dosage and frequency of administration can vary depending on whether the treatment is lactic or therapeutic. ln prophylactic applications, a relatively low dosage is administered at relatively infrequent intervals over a long period of time. Some patients ue to receive treatment for the rest of their lives. in eutic applications, a relatively high dosage at relatively short als is sometimes ed until progression of the disease is reduced or terminated, and preferably until the patient shows partial or complete ration of symptoms of disease. Thereafter, the patient can be administered a prophylactic regime.

The following examples are provided to further illustrate the invention but not to limit its scope. Other ts of the invention will be readily apparent to one of ordinary skill in the art and are encompassed by the appended claims.

Example 1: Generation of Affiniy d Factor P Antibodies A fully human phage display library was used to generate the Factor P binding antibodies described herein.

Biotinylated and non—biotinylated human and cynomolgus Factor P were used in solution and solid phase pannings. Standard panning were performed as well as RapMAT approaches (Prassler et al., (2009) lmmunotherapy 1(4):571—583). Following affinity maturation (Knappik et al., (2000) J.Mol.Biol., 296:57~86) a set of 10 antibodies were subsequently chosen for conversion to a disulfide-bridged Fab format. The resulting PCT/IBZOIZ/057394 1 29 disulfide bridged Fabs are shown in Table 1 (NV8962, NV8963, NV8964, NV8965, NV8966, NV8967).

Example 2: Further Antibody zation The following example describes methods that may be used to further optimize antibodies described herein.

Removal of Deamidation Sites Deamindation sites were identified by peptide mapping and size exclusion chromatography (SEC), run under ng conditions. The deamidated material has decreased potency in a MAC deposition assay and decreased affinity for human and cyno FP as measured by Biacore and SET. The extent of ation increased over time (3 weeks), at higher temperatures (5 days at 37C), and under ng conditions.

Deamidation can be detected using an ion—exchange coiumn resultin multiple peaks and observation of the additional, deamidated peak. Amino acid ces that are most prone to deamidation are: SNG, LNG, LNN, ELN (Daugherty, A. and Mrsny, R. (2010) Current Trends in Monoclonal Antibody Development and Manufacturing. Springer. pips-129.). Accordingly, we engaged in a series of studies to remove the deamidation sites and test the modified antibodies for ed on.

Two Fabs, NV8962 and NV8965, were re-engineered to replace a deamindation site on the heavy chain, specifically occuring at an asparagine at position 30. The ing new Fabs were generated to remove the deamindation site and corresponding amino acid replacements shown in Table 2.

Table 2: Deamidated Fabs Deaminadated Fab N30 replaced with: Modified Fab NV8962 1 30 An additional Fab that was generated replaced serine 31 with an e in Fab , generating Fab NV8962-831A. The sequences of the modified Fabs is shown in Table Removal of Cleavage Sites Further optimization was conducted on NV8962-S and NV8965-S to remove a cleavage site in the heavy chain CDRS. Specifically the heavy chain was cleaved at Y102S103.

The following table describes the amino acid substitutions that were made to destroy the cleavage site. The sequences of the modified Fabs is shown in Table 1.

Table 3: Modified Fabs NV8965-S NV8804 NV8805 Example 3: Characterization of Optimized Antibodies The following example describes methods that may be used to measure antibody affinity.

These and other methods of measuring binding ty are known in the art.

Affinity Determination Antibody affinity for Factor P was measured by surface plasmon resonance (SPR) using a Biacore T200 (Biacore) and solution equilibrium ion (SET).

Explanations of each logy and ponding mean results for Factor P binding are described below. Modelling assumptions take into account concentrations of Factor P in the , kinetics of Factor P biosynthesis and half-life, as well as the desired dosing schedule, and suggest that a Fab with an affinity of greater than 500 pM for Factor P is ient to lower levels of free Factor P. e Determination The kinetics of an interaction, i.e. the rates of complex formation (ka) and dissociation (kd), can be determined from the information in a sensorgram. if binding occurs as sample passes over a prepared sensor surface, the response in the sensorgram increases. if equilibrium is reached a constant signal will be seen.

Replacing sample with buffer causes the bound molecules to dissociate and the response decreases. Biacore evaluation software generates the values of ka and kd by fitting the data to interaction models (Table 4).

Biacore kinetic experiments were done with the BlAcore T100 (GE Healthcare) using CM5 sensor chips (GE Healthcare, BR30) at 25°C. The running buffer was HBS—EP(+) (GE Healthcare, BR—1001—88). Briefly, the following steps were carried out to determine binding affinity.

Prepare anti-FP lgG immobilized sensor chip: Mouse anti—FP monnoclonal antibody (Quidel, A235) (30ug/ml in acetate pH5.0 coupling buffer (GE Healthcare, BR51)) was d to two ent flow cells (F01 and 2) on a CM5 chip at 10ul/min flow rate for 600 seconds by using coupling procedure according to the supplier’s instruction (GE Healthcare, BR50).

The final immobilized level will be >7000RU.

Capture FP on second flow cell: tug/ml of FP in running buffer was injected at 1OUl/min on second flow cell (Fc2) to reach capture level ~20 RU for Fab or ~7 RU for lgG kinetics analysis. inject anti-FP Fab or lgG at different concentration on both flow cells: Inject anti- FP solution (0.3125nM~10nM in running buffer; at 1:2 serial dilutions) on both flow cells (F01 and 2) at 60u|lmin for 240 seconds.

Dissociation: Inject (+) running buffer at in on both flow cells to monitor the dissociation between FP and anti-FP Fab/lgG. iation time was set at 2400 seconds for 5nM and 2.5M Fab/lgG concentrations and at 300 seconds for all other concentrations including another 5nM Fab/lgG concentration.

Regeneration: Regeneration was performed at the end of each cycle on both flow cells with 10 mM Glycine-HCl pH1.7 ded by GE Healthcare)+ 0.05% P20 surfactant (GE Healthcare, BR54) at a flow rate of in for 15 seconds twice.

Kinetics analysis: Kinetic rate constants was obtained by applying 1:1 binding model with BlAevaluation 1.1 software, wherein the Rmax values were fit locally.

The results of the Biacore binding kinetics determination are shown in Table 4. As shown the antibodies described herein exhibit high affinity binding to human Factor P, with KD values typically less than or equal to 1 nM, and in many cases less than or equal to 200 pM. These dies also show very high ty to cyno Factor P (binding affinity less than 500 pM).

ZOIZ/057394 1 32 SET Determination In contrast to kinetic assays using sensor surfaces, such as SPR, SET is a method which determines affinities in solution. it is an equilibrium measurement that does not deliver kinetic data.

In SET, a constant amount of antibody is ted with different concentrations of antigen until equilibrium is reached. The concentration of free antibody in the brated solution is determined by ng the solution on an antigen coated MSDTM plate (Meso Scale DiscoveryTM) followed by incubation with an beled secondary antibody and measurement of signal intensity. At low antigen concentrations, a strong signal is achieved (high concentration of free antibody which binds to the antigen on the plate) whereas for high antigen concentration, the antibody is completely antigen- captured, resulting in a low signal. If a sufficient number of antigen concentrations in a matching range are available, the titration curve allows for a reasonable determination of the affinity, using the appropriate fit model. For a complete titration, antigen trations of at least 10-fold higher than the anticipated KD have to be applied. The constant concentration of antibody d in the assay should be in the range of, or below, the KD (Table 4).

For KD determination by solution equilibrium titration (SET), monomer fractions of antibody protein were used (at least 90% monomer content, analyzed by analytical SEC; Superdex75 (Amersham Pharmacia) for Fab, or Tosoh GBOOOSWXL (Tosoh Bioscience) for lgG, respectively). y determination in solution was basically performed as described in the literature (Friguet et al. 305-19). In order to improve the sensitivity and accuracy of the SET method, it was transferred from classical ELISA to ECL based technology (Haenel et al., 2005). 1 mg/ml goat-anti—human (Fab)2 fragment specific antibodies (Dianova) were labeled with MSD Sulfo-TAGTM NHS—Ester (Meso Scale Discovery, Gaithersburg, MD, USA) according to the manufacturer's ctions.

Human Factor P (Complement Technology cat#: A139) and Cyno Factor P purified from cyno serum (protocol adapted from Nakano, et al., (1986) J Immunol Methods 90:77—83) were coated on rd g MSD plates Scale Discovery, 384-well: MSD cat#: L21XA, 96—well: MSD cat#: L15XA) at 0.2—0.3ug/ml in 25u| PBS and incubated overnight at 4°C. Factor P inhibitors were diluted to a fixed concentration (1pM or 10pM) in incubation buffer (PBS with 2% BSA (Sigma cat#: A4503) and 1% Tween20 and 1% Triton-X (Sigma cat#: )), and added to a serial dilution of Factor P (human or cyno) in incubation buffer. Samples were allowed to reach equilibrium by incubation at RT overnight. Plates were washed 3x in wash buffer (PBS 1 33 with 0.05% Tween20), and blocked with 100ul incubation buffer at RT for 2hrs. Plates were washed 3x in wash buffer. Sample containing Factor P inhibitors and Factor P titration were added to the plate (25M), and incubated at RT for 15min. Plates were washed 3x in wash buffer. 25u| detection antibody was added (Anti-Human (Goat) Sulfo- TAG, 111000 in incubation buffer, MSD cat#: R32AJ-1), and ted at RT for 60min.

Plates were washed 3X in wash buffer, and 50u| of 1X MSD Read buffer T was added (with tant, MSD cat#: R92TC—1). Plates were read on a MSD Spector lmager 6000.

Data was analyzed using GraphPad Prism software v4, with background (an average of wells containing no Fab) subtracted from each value. X-axis values (concentration of Factor P in solution) were transformed into . KD values (KD) were fitted from the following model: Fab: Y=(Top-((Top/(2xFab))><((((1O"x)+Fab)+KD)-(((((1OAx)+Fab)+KD)><(((1OAx)+Fab)+KD)) - ((4X(10AX))XFab))"0-5)))) Top= signal at antigen concentration = O X: concentration of Factor P in solution Fab: tration of applied lent analyte (Fab) Table 4: Affinity Binding of Factor P Antibodies Factor P Factor P Biacore K; Biacore Ka Biacore Kd Antibody Species (W) (1/Ms) (1/s) NV8962 Human 46 83 1.52 X 10' 1.25 x10' 47 182 1.53 X 10 2.79 x 10 -CynoNV8965 Human 36 16 2.65 x 10' 4.10 X 10' 14 28 2.24 x 10' 6.22 x10‘ NV8963 Human 55 _ 1x10 1x104 PCT/IBZOlZ/057394 1 34 NV8967 — NV8962-Q _-_-l- ——__— WO 93762 1 35 Example 4: Factor P Antibodies Inhibit the Alternative Complement Pathway sr’s Assay ln hemolytic techniques, all of the complement components must be present and functional. Therefore hemolytic techniques can screen both functional integrity and deficiencies of the complement system (van et al., 1980;Minh et al., 1983;Tanaka et al., 1986). To measure the functional capacity of the classical pathway, sheep red blood cells coated with hemolysin (rabbit 196 to sheep red blood cells) or chicken red blood cells that are sensitized with rabbit anti-chicken antibodies are used as target cells (sensitized cells). These Ag-Ab xes te the classical pathway and result in lysis of the target cells when the components are functional and present in adequate concentration. To determine the functional capacity of the alternative y in human and cynomolgus sera, rabbit red blood cells are used as the target cell (see U.S. Pat. No. 6,087,120).

The hemolytic assay is a basic functional assay that tests for complement activation and has been used to te the ability of anti—human FP mAbs and Fab molecules to block lysis of red blood cells (RBCs) by complement pathways. In vitro and in vivo inhibition of complement activity by a single-chain Fv fragment recognizing human C5 can be ed using a haemolytic assay (Thomas et al., 1996;Rinder et al., 1995;Rinder et al., 1995) . Blockade of 05a and C5b—9 generation inhibits leukocyte and platelet activation during extracorporeal circulation. Briefly, for classical pathway assays, sensitized red blood cells (e.g., chicken RBCs) are used as targets for lysis by complement proteins present in serum. The following assay is of interest for the characterization and screening of Factor P dies for their inhibition of the alternative complement pathway.

This procedure was adapted from (Rinder et al., 1995;Thomas et al., 1996). 0 Rabbit red blood cells (Rb RBCs) — Lampire, Cat# 7246408 . Human serum — Novartis Blood Research Program; or Cyno serum — Alpha Genesis . n veronal buffer (GVB) — Boston BioProducts, Cat# lBB—300 o EGTA — Boston BioProducts, Cat# BM-151 . MgCl2 . U-bottom 96—well plate — g, Cat# 3795 o Flat-bottom 96-well plate — Corning, Cat# 3370 o NP—40 — Sigma, Cat# 74385 PCT/[B2012/057394 1 36 Protocol: Rabbit red blood cells (RBCs) were washed and adjusted to 8.33x107 cells/ml in GVB/EGTA/Mg++. 50u| Fab diluted in GVB was added to wells in a 96-well round bottom plate. 50u| serum diluted in GVB with EGTA and Mg++ was then added. Control wells were prepared in the following manner: serum wihtout Fab (negative control) and cells plus 0.1% NP-40 (100% lysis control), and NP-40 blank wells. Serum with and without Fab and controls were incubated at room temperature for 30 minutes. At that point, 30u| Rb RBCs were added to sample and control wells and 30ul of buffer was added to the blank wells. The cells were generally incubated for 30 minutes at 37°C and the plate centrifuged at m for 5min. The supernatant was harvested and transferred to a flat-bottom plate. The absorbance of the supernatant was read at OD415 and OD570. Percent hemolysis was calculated using the formula below.

(ODsampZe — ODserum .blank )— (OD ‘s —- ODbufler blank ) (y Hemol 731.30 Im.3 (OD100 %Zysz's - ODNP 40 blank )— (OD ‘s — ODbufler .biank ) Table 5 exemplifies of the ability of the Factor P antibodies and antigen binding fragments to inhibit hemolysis in 10% human or 20% cynomolgus serum. Each of the Factor P dies described herein inhibited hemolysis with an |C50 of less than or equal to 50 nM.

In contrast, when the assay was performed using sensitized red blood cells in order to examine activation of the classical complement pathway, the Factor P antibodies bed herein were found not to inhibit the classical complement pathway (data not shown). 03b Deposition Assay One method of measuring the tor activity against the ment C3 in the alternative pathway is to e its breakdown product, 03b, depositing on zymosan.

This ELISA based assay was performed according to the following steps: 25ul of 1mg/ml Zymosan A (Sigma Z4250) in carbonate buffer, pH 9.6 (Pierce Cat# 28382) was coated on Maxisorp 384—well ELISA plate (Nunc 464718) overnight at 4°C. On the following day, the zymosan—coated plate was aspirated and blocked with 100ul per well of ELISA blocking , Synblock (AbD Serotec BUF034C) for 2h at room ature. in a separate on, the inhibitors, serially diluted in gelatin veronal buffer (Boston Bioproducts ~10mM Barbital, 145mM NaCl, 0.1% Gelatin, 0.5mM MgClz, 10mM EGTA) were added to 10% serum supplemented with MgClz and EGTA for a final total reaction concentration of 1mM MgClz and 10mM EGTA. The positive control contained PCT/IBZOIZ/057394 1 37 no inhibitor and negative control had 25mM EDTA. The mixture was allowed to reach brium by incubating at room temperature for 30min. To remove the blocking buffer, the buffer was aspirated and the plate was washed once with TBS/0.05% Tween-20. 25ul per well of the 10% serum containing the inhibitors or controls was added to the plate and incubated at 37°C for 30min (previously determined by ourse to be within the linear range of C3b deposition on zymosan.) After the 30min incubation, the plate was washed three times with TBS/0.05% Tween-20. To detect 03b deposition on zymosan, 25ul per well of chicken anti—human CB-HRP conjugated polyclonal antibody (Immunology Consultants Laboratory, lnc. Cat# CC3-80P-1) diluted ing to manufacturer in PBS with 2%BSA Fraction V (Fisher Cat# lCN 80), 0.1% Tween20 (Sigma Cat# P1379), and 0.1% TritonX-100 (Sigma Cat# P234729) was added to the plate and incubate at room temperature for 1h. Afterward, the plate was washed three times with TBS/0.05% Tween-20 and then add 25ul of Ultra TMB Substrate Solution (Pierce Cat#34028.) When the solution in the well turned blue, the reaction was stoppedwith 15u| of 2N sulfuric acid. The plate was read at 450nm using the Spectromax with correction for the plastic plate at 570nm (OD 450-57%,“ reading.) The percentage of 03b deposition on zymosan was calculated using the following formula: [(013 no inhibitor - OD 25mM EDTA)‘ (OD sample - OD 25mM EDTA)] % 03b Deposition = 100 — 100 * (OD no inhibitor ' OD 25mM EDTA) Each of the antibodies tested were shown to inhibit C3b deposition with an ICSO of at least less than or equal to 10 nM (Table 5).

MAC Deposition Assay Another assay that was used to determine the ability of the Factor P antibodies to inhibit the alternative ment pathway was to e the ability of the antibodies to inhibit the generation of the ne attack complex (MAC), which is ream of the CS convertase and the activity of Factor P. Briefly, Zymosan A (Sigma ) was coated on a plate at 1mg/ml in carbonate buffer, pH 9.5, to activate the Alternative Pathway.

Fabs were pre—incubated with serum (20% serum, 5mM MgClg, 10mM EDTA), then added to the plate and incubated overnight at room temperature. After g the plate three times with TBST, MAC was ed by incubation with anti—C5b—9—ALP (Diatec) for 1h, followed by three washes with TBST, and incubation with ylumbelliferyl phosphate (Fisher) supplemented with 2mM MgClz for 30 minutes. The reaction was stopped with 0.2M EDTA, and the plate was read at ex=355nm , em=460nm. Inhibition ‘l 38 of MAC deposition was calculated for each sample relative to baseline (EDTA treated human serum) and positive control (human serum), and used to generate the IC50 curve with PRISM.

Table 5 shows data demonstrating the ability of the Factor P dies to inhibit the deposition of MAC, thus indicating that the antibodies inhibited the alternative complement pathway. Specifically, the antibodies inhibited MAC deposition with an lCSO of less than or equal to 25nM. 03a Deposition Assay Another method used to assess the ability of Factor P antibodies to inhibit the alternative complement pathway is to measure the y of antibodies to inhibit the generation of C3a following cleavage of CS by C3 tase. The assay was carried out on zymosan—coated Maxisorp plates coated at 10 mg/ml and 10% and 20% human serum pre-incubated with anti-properdin Fab diluted in a 2“ series. The serum was added to the plates for 30 s at which time the serum was collected for assessment of C3a generation.

Maxisorp plates were coated with anti-03a des-arg neo antibody (1 ug/ml) overnight, washed three times, and blocked with diluent for two hours at room temperature. ing aspiration of the diluent, serum was added for one hour. Plates were washed three times and a 100 uL/well detection antibody Mouse anti—Human C3a— Biotin 1:1000 diluted in diluent was added. Following an additional one hour incubation, a streptavidin-HRP secondary dy d 1:5000 in diluent was added to the wells for one hour at room temperature. Plates were washed four times before the addition of TMB detection substrate. The reaction was stopped using rd stop solution and absorbance was read at 450-570 nm.

In parallel to the addition of the serum, a standard curve was produced using purified 03a des—arg d in serum. ng at 5ug/ml, 03a des-arg was serially diluted 1:4 to generate a 7 point curve. The standard curve wells were treated, washed, and read as above. 2012/057394 1 39 Table 5: Functional Analysis of Factor P Antibodies Hemolytic Inhibition Zymosan- of C33 MAC. assay C3b [C50 _ generation, Factor P Antibody Factor P SpeCIes_ DeP°S‘t'°“I (nM) zooo % Serum ’ % human ECSO (nM) serum NV8962 Human 2.63 78.42 NVS965 Human 1.54 7.527 31.33 Cyno 22.17 6.36 13.20 NV8963 Human 2.34 65.08 Cyno ND 9.75 14 53 ND NV8966 Human ND 1.66 41.11 Cyno ND 6.62 13 00. N D NV8964 Human ND 1.54 42.18 Cyno NV8967 Human 2.53 43.53 C noV ND 5.92 -14.01 N D NVS962—Q Human 0.64 N D NV8962-S Human 1.53 ND Cyno 15.57 1.94 12 02 ND NV8962-T Human 1.75 C noy - -5.42 3.10 12.54 ND NV8962-G Human 1.18 ND NV8962-S31A Human 1.15 ND C noy - --7.86 3.12 11.61 ND NV8965—T 1 4O —-5.90 ND ND: Not Determined e 5: Species Cross Reactivity in order to determine whether, in addition to human and cynomolgus, the anti- Factor P antibodies described herein would bind to Factor P from other species, MAC deposition and hemolytic assays were carried out as described above. BlAcore is, or hemolytic assays were carried out as described above. The serum concentrations used for each species were as follows: 10 and 20% rabbit, 10 and 20% lgus, and and 20% human sera. Rat Factor P binding was assessed by BlAcore. As shown in Table 6 below, the Factor P antibodies were able to cross react with several species, including rabbit, rat and cynomolgus.

Table 6: Species cross-reactivity ----Rabbit Cyno PCT/IBZOlZ/057394 NV8965-S II ND: not determined Example 6: Epitope Mapping Factor P is comprised of several Thrombospondin repeat domains (TSR 0-6).

The TSRO domain is also referred to as the N terminal domain. Epitope mapping of the Factor P Fabs was performed by creating mouse and human chimeras for each TSR. us functional assays showed that the Fabs do not bind to mouse Factor P (hemolytic assays), although each of the chimeras was functional in Factor P-depleted serum. Using this method it was determined that all of the Fabs bind to TSR 5 (SEQ lD NO: 406). Figure 10 shows the dies bind region B of TSR5. The commercially- available antibody, A233, was shown not to bind in this region. Binding can be assessed by ELISA or Biacore using standard s. For one Ab, NVS487, the data was not conclusive due to cross reactivity to mouse Factor P. Sequence alignment between mouse and human Factor P TSR5 domain shows the epitope es the amino acids of SEQ ID NO: 408.

Example 7: In vivo inhibition of the Alternative Complement y Experiments were performed in cynomolgus money with antibodies of the invention to ine their ability to inhibit the alternative complement pathway.

The test item, , was administered at the dose levels shown in Table 7. The route of administration was either intravitral (NT) or intravenous (lV).

Table 7: In Vivo Study Design Dose volume Animals/group number description (pL/injection) PCT/IBZOIZ/057394 1 Control 0 (vehicle) 50llVT injection 100/IV injection 2 Low NT 1 mgleye 50/lVT (2 injection mg/mon key) 3 IV 10 100/lV mg/monkey injection 4 High IVT 5 mg/eye 50/lVT (1 0 injection key) The test item and vehicle solutions (vehicle: 10 mM His/His-HCl; 10% trehalose; 0.02% Tween 20; pH 5.5) were administered itreally and intravenously on days 1, 15, and 29 of the study as indicated in Table 7.

Assessment of toxicity was based on mortality, clinical observations, body weights, pharmacodynamics (hemolytic analysis), ophthalmic examinations, intraocular pressure ements, electroretinography, hematology, clinical chemistry, organ weights, and pathology.

There were no mortalities during the study and no test item related findings were seen after evaluation of clinical signs, body weights, ophthalmic examinations, cular pressure measurements, oretinography, hematology, clinical chemistry, organ weights, and pathology.

Complement mediated hemolytic activity was ed using the hemolytic assay described above (see Example 4). Analysis of the tic assay data showed that IV administration of NV8962 led to a complete or nearly complete but short—lived, inhibition of tic complement activity immediately after administration. When administered by the IVT route at a dose of 1 mg/eye, the test item had little or no effect on serum hemolytic complement activity. At 5 mg/eye and in 10% cynomolgus serum, a complete or nearly complete inhibition of hemolytic complement activity was observed.

WO 93762 1 43 Example 8: istic inhibition of the Alternative Complement Pathway by Antibody ations Hemo/ysis Assay Hemolytic assays using the Fab ns of the anti-CS antibody 8109 from Table 2 and anti—Factor P antibody NV8962 from Table 1 were performed as described in Example 4.

Figure 2 ifies of the ability of the Factor P antibodies and antigen binding fragments in combinations with anti-C5 antibodies and antigen binding fragments to inhibit hemolysis in 20% human serum. 500nM actor P and 500nM anti—CS Fabs individually demonstrate no inhibition of sis when incubated for 60 mins. In contrast, the combination of anti-factor P and anti-C5 antibodies at the same concentration and at concentrations as low as 167nM demonstrate near complete inhibition of hemolysis. In addition, the near complete inhibition of hemolysis lasts for up to 250 minutes.

Data from the hemolytic assay was used with Chalice Analyzer software to determine r the combination of complement inhibiting antibodies acted synergistically to block complement activation. Combination effects can be characterized by comparing each data point’s inhibition to that of a combination nce model that was derived from the single agent curves (Greco, Bravo, Parsons (1995). The search for synergy: a critical review from a response surface perspective. Pharmacol Rev 47(2): 331-85). in the Loewe additivity model (Loewe (1928). Die quantitativen Probieme der Pharmako/ogie. Ergebn. Physiol. 27: 47—187), lLoewe(Cx,CY) is the inhibition that satisfies X) + (Cy/ICY) = 1, and ley are the effective concentrations at [Loewe for the fitted single agent curves. Loewe additivity is the generally accepted reference for y (Greco et al.), as it represents the combination response generated if X and Y are the same compound.

Potency shifting is usually shown using an isobologram (Greco et al.) which shows how much less drug is required in combination to achieve a desired effect level, when compared to the single agent doses needed to reach that effect. The choice of effect level for the isobologram y and combination index calculations can either be ly or automatically ed in the Chalice er. The automatic iso-level selection algorithm finds the observed Idata with the the largest [data—[Loewe, excluding those points with [data exceeding the lesser single agent’s Imax. This exclusion is applied to ensure that the isobologram reflects the best synergy at levels covered by both single PCT/IBZOIZ/057394 1 44 agents. Having selected an isobologram level tout, the ogram is drawn by identifying the locus of concentrations that pond to crossing the chosen iso- Ievel. The isobologram shows the standard isobolographic analysis of synergy compared to the Loewe dose-additive “drug-with-itself' standard. For a specified isobologram level, the observed iso-effect contour (e.g., curved line in Figure 3) is displayed with the theoretical dose-additive contour (e.g., straight line in Figure 3), on an ICefiect-normalized linear concentration scale for both substances in the combination. The Dose—additive reference is always a line connecting the two ICefiect concentrations. The lCefiect crossing points are found by interpolating the fitted sigmoidal dose response CUl’VGS.

Potency shifting is scored as the combination index (Chou, Talalay (1984).

Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme tors. Adv Enzyme Regul 22: 27—55) CI. For a chosen iso-effect level icut, Cl. = (Ox/ECX). + (CY/ECY)|, where (Cx/ECX). for a particular data point is the ratio of the X compound’s measured concentration to its effective concentration at the chosen inhibition level. The Cl can be thought of as a rough estimate of how much drug was needed in combination relative to the single agent doses required to achieve the chosen effect level, and a value of 0.1 means that only a tenth of equivalent s of the single agents were needed for the combination to reach the same effect level. CI values in the range of 0.5—0.7 are typical for in vitro measurements of current clinical combinations (Greco et al.). A Cl value of 1.0 is indicative of an additive effect of a combination of antibodies, while a Cl value of less than 0.5 is indiciative of a synergistic effect resulting from the antibody combination. In the Chalice Analyzer, the best Cl is reported from the many combination index values calculated for each [cut crossing concentration. Among all the measured CI , the one with the largest signal-to- noise level is reported as the best combination index.

Data from the hemolytic assay were expressed as % inhibition and loaded into an 8x8 Excel table, in which the dies concentrations were sed as uM values.

The Excel te was uploaded to the Chalice re (Lehar et al. 2009) and the combination index was generated by ng an isobologram curve using ICZO for each antibody (Cl = Cx/ICX + Cy/le, where le and leare, tively, the concentrations of anti-factor P antibody and anti-C5 antibody alone that result in a 20% inhibition effect and Cx and Cy are the concentrations of each drug in the mixture that yield 20 % tion). The combination index at 20% inhibition is 0.36, indicating synergy between anti-factor P antibody and 5 antibody (Figure 3).

PCT/IBZOIZ/057394 1 45 Marcophage Infiltration The effect of anti—fP and anti—C5 Fabs individually or in combination were assessed in vivo using the poly-IC murine model of ocular ation. Mice were injected iv. with tic dsRNA analog, poly |:C in 0.1ml PBS systemically into CS7BL/6 mice along with anti-fP (antibody NV8962 from Table 1) and anti—CS antibodies (antibody 8019 from Table 2) individually or in combination. Mice were euthanized at indicated time points. Eyes and retinas were collected and protein extracts were prepared for cytokine and chemokine analysis using a multiplex assay (Pierce). To determine retinal leukocyte infiltration, eyes were fixed in 4% paraformaldehyde and stained with Alexa Fluor—488 conjugated F4/80 antibody for hages. The retinas were flat d with the retinal vasculature orientated superiorly onto a glass slide and coversliped with a drop of Vectashield mounting medium (Vector Laboratories Inc, game, CA). Fluorescent images of five (500um) regions on each retina were captured using the Axiocam MR3 camera on a Axio.lmageM1 microscope ). The number of neutrophils and macrophages was quantified with Axiovision software (Version 4.5 Zeiss). Using optical coherence tomography (OCT), images of retinas were obtained and analysed from mice treated with poly l:C. These results (Figure 4) demonstrate that at the highest concentrations tested (20ug) no greater than 45% inhibition of macrophage inhibition was observed. in contrast, combinations of the anti- Factor P and CS antibodies at concentrations as low as 2 ug demonstrated 79% inhibition and increasing the concentration achieved 100% inhibition (compared to only 13% and 32% inhibition respectively for the anti—CS and anti-Factor P antibodies dually).

Data from in vivo poly—IC model (macrophage infiltration) described in the preceding paragraph were expressed as % inhibition and loaded into a 4x4 Excel table, in which the antibody doses were expressed as ug values. The Excel template was ed to the Chalice analyzer (described above) and the combination index was generated by creating an isobologram curve using lC50 for each antibody (CI = Cx/ICX + Cy/le, where le and leare, respectively, the concentrations of anti-factor P dy and 5 antibody alone that result in a 50% inhibition effect and CX and Cy are the concentrations of each drug in the mixture that yield 50 % inhibition). The combination index at 50% tion is 0.42 (See Figure 5), indicating synergy between anti-factor P dy and anti—C5 antibody.

Claims (18)

What is claimed is

1. An isolated antibody, or antigen binding fragment, that binds Factor P, said antibody or antigen binding fragment comprising: a) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 1, 2, and 3, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 4, 5, and 6, respectively; b) heavy chain le region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N05: 85, 86, and 87, respectively, and light chain variable region 1O LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID NOs: 88, 89, and 90, respectively; 0) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N05: 99, 100, and 101, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 102, 103, and 104, 15 respectively; d) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 113, 114, and 115, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N08: 116, 117, and 118, respectively; 20 e) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID NOs: 169, 170, and 171, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N05: 172, 173, and 174, respectively; f) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ 25 ID N05: 183, 184, and 185, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 186, 187, and 188, respectively; 9) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N03: 197, 198, and 199, respectively, and light chain variable region 30 LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 200, 201, and 202, respectively; h) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N05: 211, 212, and 213, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N03: 214, 215, and 216, respectively; or i) heavy chain variable region HCDR1, HCDR2 and HCDR3 as set forth in SEQ ID N08: 225, 226, and 227, respectively, and light chain variable region LCDR1, LCDR2, and LCDR3 as set forth in SEQ ID N08: 228, 229, and 230, respectively.

The isolated antibody, or antigen g fragment, of claim 1 comprising a 1O heavy chain variable region and a light chain variable region, wherein: (a) said heavy chain variable region (1) has an amino acid sequence at least 90% identical to SEQ lD NO:175, and (2) ses an HCDR1 having the sequence of SEQ ID NO: 169; an HCDR2 having the sequence of SEQ ID NO:170; and an HCDR3 having the sequence of SEQ ID NO: 171; and 15 (b) said light chain variable region (1) has an amino acid ce at least 90% identical to SEQ lD NO:176 and (2) comprises an LCDR1 having the sequence of SEQ ID NO: 172; an LCDR2 having the sequence of SEQ ID NO:173; and an LCDR3 having the sequence of SEQ ID NO: 174.

The isolated antibody, or antigen g fragment, of claim 1 which comprises 20 a heavy chain variable region comprising SEQ ID NO: 7, 91, 105, 119, 175, 189, 203, 217, or 231 and a light chain variable region, wherein said heavy chain le region and said light chain variable region combine to form an n binding site to Factor P.

The isolated antibody, or antigen binding fragment, of claim 1 which comprises 25 a light chain variable domain comprising SEQ ID NO: 8, 92, 106, 120, 176, 190, 204, 218, or 232 and a heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable domain combine to form an antigen binding site to Factor P.

The isolated antibody, or antigen binding fragment, of claim 3 wherein said light 30 chain variable domain region comprises SEQ ID NO: 8, 92, 106, 120, 176, 190, 204, 218, or 232.

The ed antibody, or antigen binding fragment, of claim 1 which comprises a heavy chain of SEQ ID NO: 9, 93, 107, 121, 177, 191, 205, 219, or 233 and a light chain, wherein the heavy chain and the light chain combine to form an antigen binding site to Factor P.

The isolated antibody, or antigen binding fragment, of claim 1 which comprises a light chain of SEQ ID NO: 10, 94, 108, 122, 178, 192, 206, 220, or 234 and a heavy chain, wherein the light chain and the heavy chain combine to form an antigen binding site to Factor P.

The isolated antibody, or antigen g fragment, of claim 6 wherein said light 1O chain comprises SEQ ID NO: 10, 94, 108, 122, 178, 192, 206, 220, or 234.

The isolated antibody, or antigen binding fragment, of claim 2 n said isolated antibody, or antigen binding fragment, comprises a heavy chain and a light chain, said heavy chain: 15 (1) comprising said heavy chain variable ; and (2) having a sequence that is at least 90% ce identity to SEQ ID NO: 177; and said light chain: (1) comprising said light chain variable region; and 20 (2) having a sequence that is at least 90% sequence identity to SEQ ID NO: 178.

10. The antibody or antigen binding fragment of claim 1, wherein said antibody or antigen binding fragment is a human dy, a chimeric antibody, a 25 monoclonal antibody, a single chain antibody, Fab, Fab’, F(ab’)2, Fv or scFv.

11. The antibody or antigen binding fragment of claim 1, wherein said dy is an IgG isotype.

12. The isolated dy, or antigen binding fragment, of claim 1 comprising a heavy chain variable region comprising HCDR1, HCDR2 and HCDR3 each 30 having the sequence set forth in SEQ ID N03: 169, 170, and 171, respectively, and a light chain variable region comprising LCDR1, LCDR2, and LCDR3 each having the sequence set forth in SEQ ID NOs: 172, 173, and 174, respectively.

13. The antibody or antigen binding fragment of claim 12, wherein said dy or antigen binding fragment is a human antibody, a chimeric antibody, a monoclonal antibody, a single chain dy, Fab, Fab’, F(ab’)2, Fv, or scFv.

14. The antibody or antigen binding fragment of claim 12, wherein said antibody is an lgG e.

15. A composition comprising the antibody or antigen binding fragment of any one of the preceding claims and a pharmaceutically acceptable diluent or carrier.

16. The antibody or antigen binding nt of claim 1, substantially as herein described with reference to any one of the Examples and/or s thereof. 1O

17. The antibody or antigen binding fragment of any one of claims 1 to 14, substantially as herein described.

18. The composition of claim 15, substantially as herein described.