WO2013083270A3 - Method and microstructure device for the electrical contacting of biological cells - Google Patents

Method and microstructure device for the electrical contacting of biological cells Download PDF

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Publication number
WO2013083270A3
WO2013083270A3 PCT/EP2012/005018 EP2012005018W WO2013083270A3 WO 2013083270 A3 WO2013083270 A3 WO 2013083270A3 EP 2012005018 W EP2012005018 W EP 2012005018W WO 2013083270 A3 WO2013083270 A3 WO 2013083270A3
Authority
WO
WIPO (PCT)
Prior art keywords
lipid membrane
compartment
membrane
microaperture
particle
Prior art date
Application number
PCT/EP2012/005018
Other languages
German (de)
French (fr)
Other versions
WO2013083270A2 (en
Inventor
Jan Behrends
Gerhard Baaken
Srujan Kumar DONDAPATI
Original Assignee
Universitätsklinikum Freiburg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitätsklinikum Freiburg filed Critical Universitätsklinikum Freiburg
Publication of WO2013083270A2 publication Critical patent/WO2013083270A2/en
Publication of WO2013083270A3 publication Critical patent/WO2013083270A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Abstract

The invention relates to a method for the electrical contacting of at least one lipid membrane particle, particularly of a biological cell, comprising the steps; - provision of a microstructure device (1) which has at least one carrier substrate (2) having an upper side (3) with at least one microaperture (4), said microaperture having a diameter that is smaller than the diameter of the at least one lipid membrane particle to be measured, at least one first compartment (5) arranged below the microaperture, at least one second compartment (7) arranged above the microaperture, at least one first electrode (11) that is arranged in contact with the at least one first compartment, at least one second electrode (12) that is arranged in contact with the at least one second compartment, and at least one electrical control device (15); - provision of at least one first electrolyte (6) in the at least one first compartment; - formation of a lipid membrane (20) on the upper side of the carrier substrate, wherein the lipid membrane spans the at least one microaperture; - provision of at least one second electrolyte (8) in the at least one second compartment; - provision of at least one lipid membrane particle (30) above the at least one microaperture and above the lipid membrane in the at least one second compartment, wherein the lipid membrane particle has a membrane contact section (33) having a first membrane side (34) with which the lipid membrane particle lies against the lipid membrane and is supported by the carrier substrate, and a second membrane side (35) opposite the first membrane side; - generation of an ohmic contact between the at least one first electrolyte and the electrolyte (31) present on the second membrane side of the at least one lipid membrane particle through the generation of at least one voltage pulse between the at least one first and the at least one second electrode by means of the at least one control device. The invention further relates to a microstructure device that can be used for this purpose.
PCT/EP2012/005018 2011-12-06 2012-12-05 Method and microstructure device for the electrical contacting of biological cells WO2013083270A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011120394.3 2011-12-06
DE102011120394.3A DE102011120394B4 (en) 2011-12-06 2011-12-06 Method and microstructure device for electrical contacting of biological cells

Publications (2)

Publication Number Publication Date
WO2013083270A2 WO2013083270A2 (en) 2013-06-13
WO2013083270A3 true WO2013083270A3 (en) 2013-08-01

Family

ID=47429731

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/005018 WO2013083270A2 (en) 2011-12-06 2012-12-05 Method and microstructure device for the electrical contacting of biological cells

Country Status (2)

Country Link
DE (1) DE102011120394B4 (en)
WO (1) WO2013083270A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102015101425B4 (en) * 2014-10-31 2018-02-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Process for producing a component based on a structurable substrate with a three-dimensional membrane structure having pores in the nm range
EP3144674B1 (en) 2015-09-18 2020-12-02 Hochschule Kaiserslautern University of Applied Sciences Upside-down microelectrode array for single- and multicell cultures
DE102021200425A1 (en) 2021-01-18 2022-07-21 Albert-Ludwigs-Universität Freiburg, Körperschaft des öffentlichen Rechts Methods and systems for identifying a sequence of monomer building blocks of a biological or synthetic heteropolymer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006048447A1 (en) * 2004-11-05 2006-05-11 Universität Osnabrück Device and method for measuring cell properties

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7244349B2 (en) * 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006048447A1 (en) * 2004-11-05 2006-05-11 Universität Osnabrück Device and method for measuring cell properties

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PICHER ET AL: "Conducting and voltage-dependent behaviors of potassium ion channels reconstituted from diaphragm sarcoplasmic reticulum: comparison with the cardiac isoform", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - BIOMEMBRANES, ELSEVIER, AMSTERDAM, NL, vol. 1279, no. 1, 21 February 1996 (1996-02-21), pages 93 - 103, XP005891475, ISSN: 0005-2736, DOI: 10.1016/0005-2736(95)00239-1 *

Also Published As

Publication number Publication date
WO2013083270A2 (en) 2013-06-13
DE102011120394A1 (en) 2013-06-06
DE102011120394B4 (en) 2015-06-25

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