WO2013072914A3 - Plant rad52 and uses thereof - Google Patents

Plant rad52 and uses thereof Download PDF

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Publication number
WO2013072914A3
WO2013072914A3 PCT/IL2012/050460 IL2012050460W WO2013072914A3 WO 2013072914 A3 WO2013072914 A3 WO 2013072914A3 IL 2012050460 W IL2012050460 W IL 2012050460W WO 2013072914 A3 WO2013072914 A3 WO 2013072914A3
Authority
WO
WIPO (PCT)
Prior art keywords
rad52
plants
plant
present
analogues
Prior art date
Application number
PCT/IL2012/050460
Other languages
French (fr)
Other versions
WO2013072914A2 (en
Inventor
Avraham A. Levy
Aviva Samach
Shmuel Pietrokovski
Original Assignee
Yeda Research And Development Co. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Research And Development Co. Ltd. filed Critical Yeda Research And Development Co. Ltd.
Publication of WO2013072914A2 publication Critical patent/WO2013072914A2/en
Publication of WO2013072914A3 publication Critical patent/WO2013072914A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention relates to compositions and methods useful in genetic manipulation of plants. Particularly, the present invention discloses the presence of RAD52 analogues in plants and provides genetically modified plants having altered homologous recombination and DNA repair processes.
PCT/IL2012/050460 2011-11-17 2012-11-15 Plant rad52 and uses thereof WO2013072914A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161560841P 2011-11-17 2011-11-17
US61/560,841 2011-11-17

Publications (2)

Publication Number Publication Date
WO2013072914A2 WO2013072914A2 (en) 2013-05-23
WO2013072914A3 true WO2013072914A3 (en) 2013-08-01

Family

ID=47428787

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2012/050460 WO2013072914A2 (en) 2011-11-17 2012-11-15 Plant rad52 and uses thereof

Country Status (1)

Country Link
WO (1) WO2013072914A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110846239B (en) * 2019-11-29 2022-08-23 南京工业大学 Recombinant yarrowia lipolytica with high homologous recombination efficiency as well as construction method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022811A2 (en) * 2000-09-15 2002-03-21 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agrifood Canada Modulation of meiotic recombination
US20060150283A1 (en) * 2004-02-13 2006-07-06 Nickolai Alexandrov Sequence-determined DNA fragments and corresponding polypeptides encoded thereby

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
US6541684B1 (en) 1998-02-13 2003-04-01 Trustees Of Columbia University In The City Of New York Nucleotide sequences encoding maize RAD51
AUPQ901500A0 (en) 2000-07-27 2000-08-17 Rhobio Composition and method for increased meiotic recombination in plants
US20030121074A1 (en) 2001-12-20 2003-06-26 Boyce Thompson Institute For Plant Research Plant XRCC3 genes and methods of use
US6906243B2 (en) 2001-12-20 2005-06-14 Boyce Thompson Institute For Plant Research Plant MSH2 sequences and methods of use
US7892823B2 (en) 2007-02-06 2011-02-22 Geneprotech, Inc. Methods and systems for high homologous recombination (“HR”) targeting efficiency

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002022811A2 (en) * 2000-09-15 2002-03-21 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agrifood Canada Modulation of meiotic recombination
US20060150283A1 (en) * 2004-02-13 2006-07-06 Nickolai Alexandrov Sequence-determined DNA fragments and corresponding polypeptides encoded thereby

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A. SAMACH ET AL: "Identification of Plant RAD52 Homologs and Characterization of the Arabidopsis thaliana RAD52-Like Genes", THE PLANT CELL ONLINE, vol. 23, no. 12, 1 December 2011 (2011-12-01), pages 4266 - 4279, XP055052763, ISSN: 1040-4651, DOI: 10.1105/tpc.111.091744 *
MORTENSEN U H ET AL: "Rad52", CURRENT BIOLOGY, CURRENT SCIENCE, GB, vol. 19, no. 16, 25 August 2009 (2009-08-25), pages R676 - R677, XP026498316, ISSN: 0960-9822, [retrieved on 20090824], DOI: 10.1016/J.CUB.2009.06.001 *
SABINA JANICKA ET AL: "A RAD52-like single-stranded DNA binding protein affects mitochondrial DNA repair by recombination", THE PLANT JOURNAL, vol. 72, no. 3, 30 August 2012 (2012-08-30), pages 423 - 435, XP055054241, ISSN: 0960-7412, DOI: 10.1111/j.1365-313X.2012.05097.x *

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