WO2013067259A3 - Regulatory nucleic acids and methods of use - Google Patents

Regulatory nucleic acids and methods of use Download PDF

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Publication number
WO2013067259A3
WO2013067259A3 PCT/US2012/063169 US2012063169W WO2013067259A3 WO 2013067259 A3 WO2013067259 A3 WO 2013067259A3 US 2012063169 W US2012063169 W US 2012063169W WO 2013067259 A3 WO2013067259 A3 WO 2013067259A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
methods
regulatory nucleic
regulatory elements
expression
Prior art date
Application number
PCT/US2012/063169
Other languages
French (fr)
Other versions
WO2013067259A2 (en
Inventor
Michael Nuccio
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2011/059123 external-priority patent/WO2012061585A2/en
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of WO2013067259A2 publication Critical patent/WO2013067259A2/en
Publication of WO2013067259A3 publication Critical patent/WO2013067259A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8269—Photosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1294—Phosphotransferases with paired acceptors (2.7.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Abstract

The present invention relates generally to the field of molecular biology and describes nucleic acids encoding regulatory elements capable of affecting expression of a coding sequence. The regulatory elements described herein may be used to direct the expression of a heterologous coding region in the green tissues and upon exposure to light in plants. The invention may also be used to create transgenic plants having increased yield.
PCT/US2012/063169 2011-11-03 2012-11-02 Regulatory nucleic acids and methods of use WO2013067259A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2011/059123 WO2012061585A2 (en) 2010-11-04 2011-11-03 In silico prediction of high expression gene combinations and other combinations of biological components
USPCT/US2011/059123 2011-11-03

Publications (2)

Publication Number Publication Date
WO2013067259A2 WO2013067259A2 (en) 2013-05-10
WO2013067259A3 true WO2013067259A3 (en) 2013-08-15

Family

ID=47178358

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2012/063169 WO2013067259A2 (en) 2011-11-03 2012-11-02 Regulatory nucleic acids and methods of use
PCT/US2012/063161 WO2013067252A1 (en) 2010-11-04 2012-11-02 Polynucleotides, polypeptides and methods for enhancing photossimilation in plants

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2012/063161 WO2013067252A1 (en) 2010-11-04 2012-11-02 Polynucleotides, polypeptides and methods for enhancing photossimilation in plants

Country Status (6)

Country Link
CN (1) CN103998611A (en)
AU (1) AU2012332343A1 (en)
CA (1) CA2853490A1 (en)
HU (1) HUP1400505A2 (en)
MX (1) MX2014005375A (en)
WO (2) WO2013067259A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001083790A2 (en) * 2000-05-01 2001-11-08 Monsanto Technology Llc Plant regulatory sequences for selective control of gene expression
US20070209085A1 (en) * 2004-09-01 2007-09-06 Kunsheng Wu Zea mays ribulose bisphosphate carboxylase activase promoter
US20080263730A1 (en) * 1999-05-07 2008-10-23 Andersen Scott E Nucleic acid molecules and other molecules associated with plants
WO2009091518A2 (en) * 2008-01-15 2009-07-23 Monsanto Technology, Llc Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
WO2009146015A2 (en) * 2008-03-31 2009-12-03 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
WO2010028456A1 (en) * 2008-09-15 2010-03-18 Agriculture Victoria Services Pty Ltd Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant
WO2012061585A2 (en) * 2010-11-04 2012-05-10 Syngenta Participations Ag In silico prediction of high expression gene combinations and other combinations of biological components

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
JP2615013B2 (en) 1985-08-07 1997-05-28 モンサント コンパニ− Glyphosate resistant chimeric gene
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ES2018274T5 (en) 1986-03-11 1996-12-16 Plant Genetic Systems Nv VEGETABLE CELLS RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS, PREPARED BY GENETIC ENGINEERING.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
US5637489A (en) 1986-08-23 1997-06-10 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
US4940935A (en) 1989-08-28 1990-07-10 Ried Ashman Manufacturing Automatic SMD tester
ES2187497T3 (en) 1990-04-12 2003-06-16 Syngenta Participations Ag PROMOTERS PREFERREDLY IN FABRICS.
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5187267A (en) 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
US5266317A (en) 1990-10-04 1993-11-30 University Of Georgia Research Foundation, Inc. Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions
FR2673642B1 (en) 1991-03-05 1994-08-12 Rhone Poulenc Agrochimie CHIMERIC GENE COMPRISING A PROMOTER CAPABLE OF GIVING INCREASED TOLERANCE TO GLYPHOSATE.
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
AU668096B2 (en) 1991-08-27 1996-04-26 Syngenta Participations Ag Proteins with insecticidal properties against homopteran insects and their use in plant protection
US5994629A (en) 1991-08-28 1999-11-30 Novartis Ag Positive selection
GB9304200D0 (en) 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
US5607914A (en) 1993-01-13 1997-03-04 Pioneer Hi-Bred International, Inc. Synthetic antimicrobial peptides
RU2143496C1 (en) 1993-06-30 1999-12-27 Моген Интернэшнл Н.В. Trehalose producing in plants
US5580852A (en) 1993-12-17 1996-12-03 Pioneer Hi-Bred International, Inc. Derivatives of tachyplesin having inhibitory activity towards plant pathogenic fungi
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
US6100450A (en) 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
IL138191A0 (en) 1998-03-11 2001-10-31 Novartis Ag Expression of trehalose biosynthetic genes in plants
GB9816639D0 (en) 1998-07-30 1998-09-30 Novartis Ag Organic compounds
WO2002016655A2 (en) 2000-08-24 2002-02-28 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
US7695968B2 (en) 2003-03-12 2010-04-13 Evogene Ltd. Nucleotide sequences regulating gene expression and constructs and methods utilizing same
WO2005021723A2 (en) 2003-08-27 2005-03-10 Syngenta Participations Ag Nucleic acid molecules from rice controlling abiotic stress tolerance
US20060260011A1 (en) 2005-03-28 2006-11-16 Syngenta Participations Ag Methods and genetic constructs for modification of lignin composition of corn cobs
US8129588B2 (en) 2004-04-20 2012-03-06 Syngenta Participations Ag Regulatory sequences for expressing gene products in plant reproductive tissue
ES2429430T3 (en) 2005-01-14 2013-11-14 University Of Guelph Gene and protein for the detection of nitrogen regulated sugars and their modulation
EP2183372A2 (en) * 2007-07-31 2010-05-12 BASF Plant Science GmbH Plants having enhanced yield-related traits and a method for making the same
CN101173002B (en) 2007-10-29 2010-08-25 中国科学院遗传与发育生物学研究所 Plants stress tolerance correlation transcription factor GmWRKY54, encoding gene and application thereof
WO2010031312A1 (en) 2008-09-16 2010-03-25 Institute Of Genetics And Development Biology, Chinese Academy Of Sciences Regulation of nitrogen starvation response
US20110030099A1 (en) 2009-07-17 2011-02-03 John Andrew Ryals Plants and modulators for improved drought tolerance
JP5937635B2 (en) 2014-03-28 2016-06-22 ファナック株式会社 Motor driving device having welding detection function of magnetic contactor

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080263730A1 (en) * 1999-05-07 2008-10-23 Andersen Scott E Nucleic acid molecules and other molecules associated with plants
WO2001083790A2 (en) * 2000-05-01 2001-11-08 Monsanto Technology Llc Plant regulatory sequences for selective control of gene expression
US20070209085A1 (en) * 2004-09-01 2007-09-06 Kunsheng Wu Zea mays ribulose bisphosphate carboxylase activase promoter
WO2009091518A2 (en) * 2008-01-15 2009-07-23 Monsanto Technology, Llc Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits
WO2009146015A2 (en) * 2008-03-31 2009-12-03 Ceres, Inc. Promoter, promoter control elements, and combinations, and uses thereof
WO2010028456A1 (en) * 2008-09-15 2010-03-18 Agriculture Victoria Services Pty Ltd Modification of fructan biosynthesis, increasing plant biomass, and enhancing productivity of biochemical pathways in a plant
WO2012061585A2 (en) * 2010-11-04 2012-05-10 Syngenta Participations Ag In silico prediction of high expression gene combinations and other combinations of biological components

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 23 November 1989 (1989-11-23), "Maize mRNA for phosphoenolpyruvate carboxylase (EC 4.1.1.31)", XP002697445, retrieved from EBI accession no. EM_STD:X15238 Database accession no. X15238 *
DATABASE EMBL [Online] 25 August 2003 (2003-08-25), "PUIHW27TB ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa0587E05, genomic survey sequence.", XP002697446, retrieved from EBI accession no. EM_GSS:CG160617 Database accession no. CG160617 *
DATABASE EMBL [online] 26 November 2001 (2001-11-26), "Sequence 84 from Patent WO0183790.", XP002697449, retrieved from EBI accession no. EM_PAT:AX299943 Database accession no. AX299943 *
DATABASE EMBL [Online] 28 August 2003 (2003-08-28), "OG1DG24TV ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0736C23, genomic survey sequence.", XP002697444, retrieved from EBI accession no. EM_GSS:CG247449 Database accession no. CG247449 *
DATABASE EMBL [Online] 29 December 2006 (2006-12-29), "Zea mays chromosome 5 clone ZMMBBb-265N23; ZMMBBb0265N23, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.", XP002697447, retrieved from EBI accession no. EM_HTG:AC195910 Database accession no. AC195910 *
DATABASE EMBL [online] 31 October 2008 (2008-10-31), "Zea mays clone 243130 phosphoribulokinase mRNA, complete cds.", XP002697450, retrieved from EBI accession no. EM_STD:EU962495 Database accession no. EU962495 *
DATABASE EMBL [Online] 4 March 2003 (2003-03-04), "OGFAF43TC ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0238G14, DNA sequence.", XP002697454, retrieved from EBI accession no. EM_GSS:BZ728810 Database accession no. BZ728810 *
DATABASE EMBL [Online] 7 November 2002 (2002-11-07), "ia55d06.g1 WGS-ZmaysF (JM107 adapted methyl filtered) Zea mays genomic clone ia55d06 5', DNA sequence.", XP002690889, retrieved from EBI accession no. EM_GSS:BZ321679 Database accession no. BZ321679 *
DATABASE Geneseq [online] 10 December 2009 (2009-12-10), "Zea mays DNA sequence, SEQ ID 11497.", XP002697451, retrieved from EBI accession no. GSN:AXG74984 Database accession no. AXG74984 *
DATABASE Geneseq [online] 3 February 2011 (2011-02-03), "Maize LIB189 cDNA library EST SEQ ID NO:23385.", XP002697448, retrieved from EBI accession no. GSN:AUQ72529 Database accession no. AUQ72529 *
NICKOLAI N ALEXANDROV ET AL: "Insights into corn genes derived from large-scale cDNA sequencing", PLANT MOLECULAR BIOLOGY, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NL, vol. 69, no. 1-2, 21 October 2008 (2008-10-21), pages 179 - 194, XP019648179, ISSN: 1573-5028 *
P. M. GILMARTIN: "Molecular Light Switches for Plant Genes", THE PLANT CELL ONLINE, vol. 2, no. 5, 1 May 1990 (1990-05-01), pages 369 - 378, XP055048800, ISSN: 1040-4651, DOI: 10.1105/tpc.2.5.369 *
RICHARD L HUDSPETH ET AL: "Structure and expression of the maize gene encoding the phosphoenolpyruvate carboxylase isozyme involved in C4 photosynthesis", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 12, no. 5, 1 May 1989 (1989-05-01), pages 579 - 589, XP001317951, ISSN: 0167-4412 *
ZHENLONG XU ET AL: "Characterization of promoter expression patterns of OsNrt2.1, a nitrate transporter gene of rice (Oryza sativa L.)", FRONTIERS OF AGRICULTURE IN CHINA, SP HIGHER EDUCATION PRESS, vol. 3, no. 4, 1 December 2009 (2009-12-01), pages 402 - 412, XP007921416, ISSN: 1673-7334, DOI: 10.1007/S11703-009-0047-5 *

Also Published As

Publication number Publication date
MX2014005375A (en) 2014-10-17
CN103998611A (en) 2014-08-20
WO2013067259A2 (en) 2013-05-10
CA2853490A1 (en) 2013-05-10
WO2013067252A1 (en) 2013-05-10
AU2012332343A1 (en) 2014-05-22
HUP1400505A2 (en) 2015-03-02

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