WO2013063478A4 - Bioconversion of biomass to ethanol - Google Patents

Bioconversion of biomass to ethanol Download PDF

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Publication number
WO2013063478A4
WO2013063478A4 PCT/US2012/062236 US2012062236W WO2013063478A4 WO 2013063478 A4 WO2013063478 A4 WO 2013063478A4 US 2012062236 W US2012062236 W US 2012062236W WO 2013063478 A4 WO2013063478 A4 WO 2013063478A4
Authority
WO
WIPO (PCT)
Prior art keywords
biomass
slurry
acid
produce
ethanol
Prior art date
Application number
PCT/US2012/062236
Other languages
French (fr)
Other versions
WO2013063478A1 (en
Inventor
Renata Bura
Shannon EWANICK
Original Assignee
Treefree Biomass Solutions, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Treefree Biomass Solutions, Inc. filed Critical Treefree Biomass Solutions, Inc.
Publication of WO2013063478A1 publication Critical patent/WO2013063478A1/en
Publication of WO2013063478A4 publication Critical patent/WO2013063478A4/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a method for the conversion of a biomass to ethanol that includes increasing the moisture content of the biomass. The invention may be employed with any of a variety of biomasses, such as Arundo donax.

Claims

AMENDED CLAIMS received by the International Bureau on 30 April 2013 (30.04.2013)
1. A method for producing ethanol from a biomass comprising:
a) increasing the moisture content of the biomass by soaking the biomass in water for about 6 to about 36 hours;
b) pretreating the biomass with an acid-catalyzed steam explosion to produce a slurry; and
c) subjecting the slurry to i) enzymatic hydrolysis and ii) fermentation by a microorganism to produce ethanol.
2. The method according to claim 1 , wherein the biomass is totally submerged in the water.
3. The method according to claim 1 , wherein the period of time is about 24 hours.
4. The method according to claim 1 , wherein the acid- catalyzed steam explosion utilizes an acid selected from sulfur dioxide and sulfuric acid.
5. A method for producing ethanol from a biomass comprising:
a) increasing the moisture content of the biomass by soaking the biomass in water for a period of time;
b) pretreating the biomass with an acid-catalyzed steam explosion to produce a slurry; and
c) subjecting the slurry to simultaneous saccharification and fermentation (SSF) by subjecting the slurry to i) enzymatic hydrolysis and ii) fermentation by a microorganism to produce ethanol.
6. The method according to claim 1 , wherein the microorganism is S. cerevisiae.
7. The method according to claim 1 , wherein the moisture content of the biomass after step (a) is 10-60%.
8. A method for producing ethanol from an Arundo donax biomass comprising:
a) increasing the moisture content of the Arundo donax biomass by soaking the Arundo donax biomass in water for a period of time;
b) pretreating the Arundo donax biomass with an acid- catalyzed steam explosion to produce a slurry; and
c) subjecting the slurry to i) enzymatic hydrolysis and ii) fermentation by a microorganism to produce ethanol.
9. A method for producing ethanol from a biomass comprising:
a) increasing the moisture content of the biomass by steaming the biomass for a period of time;
b) pretreating the biomass with an acid-catalyzed steam explosion to produce a slurry; and c) subjecting the slurry to i) enzymatic hydrolysis and ii) fermentation by a microorganism to produce ethanol.
10. The method according to claim 9, wherein the steaming occurs in a steam gun.
11. The method according to claim 9, wherein the period of time is from about 15 seconds to about 120 seconds.
12. The method according to claim 11 , wherein the period of time is about 45 seconds to about 90 seconds.
13. The method according to claim 9, wherein the acid- catalyzed steam explosion utilizes an acid selected from sulfur dioxide and sulfuric acid.
14. The method according to claim 8, wherein step c) comprises simultaneous saccharification and fermentation (SSF).
15. The method according to claim 9, wherein the
microorganism is S. ceravisiae.
16. The method according to claim 9, wherein the moisture content of the blomass after step (a) is about 10-60%.
17. The method according to claim 9, wherein the biomass is Arundo donax.
PCT/US2012/062236 2011-10-28 2012-10-26 Bioconversion of biomass to ethanol WO2013063478A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161552750P 2011-10-28 2011-10-28
US61/552,750 2011-10-28

Publications (2)

Publication Number Publication Date
WO2013063478A1 WO2013063478A1 (en) 2013-05-02
WO2013063478A4 true WO2013063478A4 (en) 2013-06-20

Family

ID=48168592

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/062236 WO2013063478A1 (en) 2011-10-28 2012-10-26 Bioconversion of biomass to ethanol

Country Status (1)

Country Link
WO (1) WO2013063478A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3241907T3 (en) 2016-05-03 2019-04-15 Beta Renewables Spa PROCEDURE FOR MANUFACTURING A BIO PRODUCT
FR3053969B1 (en) * 2016-07-18 2019-12-20 IFP Energies Nouvelles PROCESS OF TREATING LIGNOCELLULOSIC BIOMASS BY IMPREGNATION AND EXPLOSION WITH STEAM
DE102017125090A1 (en) 2016-10-27 2018-05-03 PTT Global Chemical Public Company Ltd. Pretreatment process of lignocellulosic biomass with alkaline solution and steam explosion
FR3069248B1 (en) * 2017-07-19 2020-07-31 Ifp Energies Now LIGNO-CELLULOSIC BIOMASS TREATMENT PROCESS.
CN116555359B (en) * 2023-06-25 2023-12-08 齐鲁工业大学(山东省科学院) Method for producing fuel ethanol by bioconversion of poplar biomass

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0608369A2 (en) * 2005-03-17 2010-11-16 Novozymes North America Inc process for the production of a fermentation product from lignocellulosic material
WO2008063512A2 (en) * 2006-11-13 2008-05-29 Pyramid Bioenergy Llc Methods, compositions and systems related to ethanol manufactured from the grass arundo donax
US20090117633A1 (en) * 2007-11-05 2009-05-07 Energy Enzymes Inc. Process of Producing Ethanol Using Starch with Enzymes Generated Through Solid State Culture
CN103221547B (en) * 2010-11-09 2015-12-09 绿源乙醇公司 For producing the continuation method of ethanol from lignocellulosic biomass

Also Published As

Publication number Publication date
WO2013063478A1 (en) 2013-05-02

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