WO2013052717A3 - Microorganisms and methods for producing acrylate and other products from homoserine - Google Patents

Microorganisms and methods for producing acrylate and other products from homoserine Download PDF

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Publication number
WO2013052717A3
WO2013052717A3 PCT/US2012/058826 US2012058826W WO2013052717A3 WO 2013052717 A3 WO2013052717 A3 WO 2013052717A3 US 2012058826 W US2012058826 W US 2012058826W WO 2013052717 A3 WO2013052717 A3 WO 2013052717A3
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WO
WIPO (PCT)
Prior art keywords
microorganisms
methods
homoserine
products
acrylate
Prior art date
Application number
PCT/US2012/058826
Other languages
French (fr)
Other versions
WO2013052717A2 (en
Inventor
Jun Xu
Charles Winston Saunders
Phillip Richard Green
Juan Esteban VELASQUEZ
Original Assignee
The Procter & Gamble Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Procter & Gamble Company filed Critical The Procter & Gamble Company
Priority to CA2849823A priority Critical patent/CA2849823A1/en
Priority to CN201280048633.9A priority patent/CN103890186A/en
Priority to EP12780579.4A priority patent/EP2764111A2/en
Priority to AU2012318597A priority patent/AU2012318597A1/en
Publication of WO2013052717A2 publication Critical patent/WO2013052717A2/en
Publication of WO2013052717A3 publication Critical patent/WO2013052717A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01003—Aldehyde dehydrogenase (NAD+) (1.2.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04002—Oxoglutarate dehydrogenase (succinyl-transferring) (1.2.4.2), i.e. alpha-ketoglutarat dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03002—L-Amino-acid oxidase (1.4.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03003—D-Amino-acid oxidase (1.4.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01072—Branched-chain-2-oxoacid decarboxylase (4.1.1.72)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

This invention relates to microorganisms that convert a carbon source to acrylate or other desirable products using homoserine and 2-keto-4-hydroxybutyrate as intermediates. The invention provides genetically engineered microorganisms that carry out the conversion, as well as methods for producing acrylate by culturing the microorganisms. Also provided are microorganisms and methods for converting homoserine to 3-hydroxypropionyl-CoA, 3-hydroxypropionate (3HP), poly-3-hydroxypropionate and 1,3-propanediol.
PCT/US2012/058826 2011-10-05 2012-10-05 Microorganisms and methods for producing acrylate and other products from homoserine WO2013052717A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2849823A CA2849823A1 (en) 2011-10-05 2012-10-05 Microorganisms and methods for producing acrylate and other products from homoserine
CN201280048633.9A CN103890186A (en) 2011-10-05 2012-10-05 Microorganisms and methods for producing acrylate and other products from homoserine
EP12780579.4A EP2764111A2 (en) 2011-10-05 2012-10-05 Microorganisms and methods for producing acrylate and other products from homoserine
AU2012318597A AU2012318597A1 (en) 2011-10-05 2012-10-05 Microorganisms and methods for producing acrylate and other products from homoserine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161543511P 2011-10-05 2011-10-05
US61/543,511 2011-10-05

Publications (2)

Publication Number Publication Date
WO2013052717A2 WO2013052717A2 (en) 2013-04-11
WO2013052717A3 true WO2013052717A3 (en) 2013-06-13

Family

ID=47116373

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/058826 WO2013052717A2 (en) 2011-10-05 2012-10-05 Microorganisms and methods for producing acrylate and other products from homoserine

Country Status (5)

Country Link
EP (1) EP2764111A2 (en)
CN (1) CN103890186A (en)
AU (1) AU2012318597A1 (en)
CA (1) CA2849823A1 (en)
WO (1) WO2013052717A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2922120C (en) 2013-09-03 2022-09-06 Myriant Corporation A process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol
EP2930244A1 (en) * 2014-04-07 2015-10-14 The Procter and Gamble Company Microorganisms and methods for producing acrylate and other products from homoserine
WO2019152755A1 (en) * 2018-02-01 2019-08-08 Invista North America S.A.R.L. Methods and materials for the biosynthesis of beta hydroxy acids and/or derivatives thereof and/or compounds related thereto
CN110777127A (en) * 2019-11-28 2020-02-11 江南大学 Application of a branched-chain amino acid transaminase as reductase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004038030A2 (en) * 2002-05-10 2004-05-06 Metabolix, Inc. Bioabsorbable polymer containing 2-hdroxyacid monomers
WO2007042494A2 (en) * 2005-10-10 2007-04-19 Evonik Degussa Gmbh Microbiological production of 3-hydroxypropionic acid
WO2010076324A1 (en) * 2008-12-31 2010-07-08 Metabolic Explorer Method for the preparation of diols

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2801058B1 (en) 1999-11-16 2002-01-18 Roquette Freres PROCESS FOR THE PURIFICATION OF 1,3-PROPANEDIOL FROM A FERMENTATION MEDIUM
TWI456949B (en) 2008-04-30 2014-10-11 Thomson Licensing System and method for switching between phone services
WO2010085731A2 (en) 2009-01-23 2010-07-29 Microbia, Inc. Production of 1,4 butanediol in a microorganism
DE102009035091A1 (en) 2009-07-28 2011-02-10 Mahle International Gmbh Position sensor and linear actuator
US20110125118A1 (en) 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an Organic Acid and/or Related Chemicals

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004038030A2 (en) * 2002-05-10 2004-05-06 Metabolix, Inc. Bioabsorbable polymer containing 2-hdroxyacid monomers
WO2007042494A2 (en) * 2005-10-10 2007-04-19 Evonik Degussa Gmbh Microbiological production of 3-hydroxypropionic acid
WO2010076324A1 (en) * 2008-12-31 2010-07-08 Metabolic Explorer Method for the preparation of diols

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KOROTKOVA NATALIA ET AL: "Glyoxylate regeneration pathway in the methylotroph Methylobacterium extorquens AM1", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC; US, vol. 184, no. 6, 1 March 2002 (2002-03-01), pages 1750 - 1758, XP002496577, ISSN: 0021-9193, DOI: 10.1128/JB.184.6.1750-1758.2002 *

Also Published As

Publication number Publication date
CA2849823A1 (en) 2013-04-11
AU2012318597A1 (en) 2014-04-24
AU2012318597A2 (en) 2014-11-06
EP2764111A2 (en) 2014-08-13
CN103890186A (en) 2014-06-25
WO2013052717A2 (en) 2013-04-11

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