WO2013048251A1 - Procédés et usages améliorés liés à l'élevage d'un insecte zoophytophage et protection des récoltes - Google Patents

Procédés et usages améliorés liés à l'élevage d'un insecte zoophytophage et protection des récoltes Download PDF

Info

Publication number
WO2013048251A1
WO2013048251A1 PCT/NL2012/050683 NL2012050683W WO2013048251A1 WO 2013048251 A1 WO2013048251 A1 WO 2013048251A1 NL 2012050683 W NL2012050683 W NL 2012050683W WO 2013048251 A1 WO2013048251 A1 WO 2013048251A1
Authority
WO
WIPO (PCT)
Prior art keywords
carbohydrate
zoophytophagous
insect
eggs
macrolophus
Prior art date
Application number
PCT/NL2012/050683
Other languages
English (en)
Inventor
Karel Jozef Florent Bolckmans
Alberto URBANEJA GARCIA
Miquel ALONSO VALIENTE
Alejandro TENA BARREDA
Pablo URBANEJA BERNAT
Radbout TIMMER
Original Assignee
Koppert B.V.
Instituto Valenciano De Investigaciones Agrarias (Ivia)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koppert B.V., Instituto Valenciano De Investigaciones Agrarias (Ivia) filed Critical Koppert B.V.
Publication of WO2013048251A1 publication Critical patent/WO2013048251A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms

Definitions

  • the present invention relates to the field of biological crop protection.
  • the invention relates to a method for rearing a zoophytophagous insect.
  • Further aspects of the invention relate to a method for crop protection, a method for reducing crop damage caused by a zoophytophagous insect, the use of a carbohydrate source as a nutritional source for a zoophytophagous insect, compositions comprising a carbohydrate source for specific uses in relation to the development of a zoophytophagous insect and the use of a carbohydrate source as a food source for a zoophytophagous insect.
  • Zoophytophagous insects are insects which have omnivorous feeding habits and can feed on both prey and plant tissue.
  • the dependency on prey and plant tissue may differ between the species of zoophytophagous insect and for a certain species may differ depending on the environmental conditions, such as availability of prey.
  • Species having a prominent predatory behaviour have a beneficial utility in agriculture and horticulture as biological crop protection tools, due to their preying on crop pests.
  • Species having a prominent feeding on plant tissue are potential pests for crops produced in agriculture and/or horticulture.
  • Nesidiocoris tenuis Reuter (Hemiptera: Miridae) is an example of a zoophytophagous mirid with biological control potential that commonly appears in tomato crops in the Mediterranean (Arno et al. 2010b). This predator is mass-reared and it has been primarily released to control whiteflies (Hemiptera: Aleyrodidae) in greenhouses (Calvo et al. 2009, Urbaneja et al. 2005, Gabarra et al. 2008, Castane et al. 2008).
  • Nesidiocoris tenuis was also detected preying upon the invasive pest, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), immediately after its detection in the Spanish Mediterranean coastal area (Desneux et al. 2010), on which it has been shown to be able to regulate its populations (Molla et al. 2011, Gonzalez-Cabrera et al. 2011).
  • N tenuis has also been observed to contribute to the control of thrips, leafminers, spidermites and other lepidopteran pests in greenhouses (Jacas et al. 2008).
  • Nesidiocoris tenuis can make significant contributions to control of tomato greenhouse pests mentioned above. However, by feeding directly on vegetative and reproductive parts of the plant it may cause necrotic rings on stems and leaf petioles, flower abortion. In addition it can also puncture fruits, such as tomato fruits, possibly reducing yields (Sanchez 2008).
  • N. tenuis 1-2 individuals/m 2
  • This strategy has been successfully used to control whitefly populations once a certain number of N. tenuis is present in the crop.
  • establishment of this number requires five to eight weeks from the release in spring- summer crops.
  • E. kuehniella eggs which can be rather expensive.
  • the problem of costs of E. kuehniella eggs is also encountered in the rearing of phytophagous insects, such as mirids. In the rearing processes for these organisms E. kuehniella eggs are also used as a food source. For this reason, providing further alternative diets for mirids would be beneficial.
  • N. tenuis Apart from N. tenuis other predatory species from the family of the Miridae are also used for biological crop protection. These related predators from the family of the Miridae have a similar developmental biology as N. tenuis. For example Macrolophus pygmaeus and Macrolophus caliginosus are also commercially available. The family of the Miridae has promise to provide additional commercially valuable predators, especially from the genera Nesidiocoris, Macrolophus, Engytatus, Dicyphus and Deraeocoris. Tests using the mirid N.
  • tenuis as a model insect for zoophytophagous insects have surprisingly shown that when this model insect is provided with a carbohydrate source in the presence of alternative food sources known to be well accepted, such as E. kuehniella eggs or plant tissue, the consumption of these alternative food sources decreases. It has surprisingly been shown that provision of a carbohydrate source reduces the amount of E. kuehniella eggs required in mass rearing of this zoophytophagous mirid and/or for its establishment in a crop. Furthermore it has been found that provision of a carbohydrate source to the zoophytophagous mirid N. tenuis has a positive effect on parameters related to population development, such as fecundity.
  • the invention according to an aspect relates to a method for rearing a zoophytophagous insect comprising:
  • a food source comprising protein, such as animal protein
  • the population of the zoophytophagous insect preferably comprises sexually mature individuals of both sexes and/or comprises individuals of both sexes that can develop to sexual maturity. Individuals that can develop to sexual maturity include all pre-mature life stages of the zoophytophagous insect, including fertilized eggs and nymphs.
  • the method according to the invention is aimed at rearing a zoophytophagous insect.
  • zoophytophagous insects are insects which have omnivorous feeding habits and can feed on both prey and plant tissue. Certain species of zoophytophagous insects are classified as beneficial, due to extensive preying on crop pests, other zoophytophagous insects are classified as pests due to extensive feeding on plant tissue.
  • beneficial and pest zoophytophagous insects Rearing of both beneficial and pest zoophytophagous insects is of interest to agriculture and horticulture.
  • Beneficial zoophytophagous insects may be reared as biological crop protection tools.
  • the zoophytophagous insects regarded as (potential) crop pests may be reared to conduct research into for example their behaviour, reproduction and control. Rearing of a zoophytophagous insect suitable for use as a crop protection agent is preferred for the present invention.
  • the zoophytophagous insect preferably is a zoophytophagous mirid, meaning that it is selected from the family of the Miridae.
  • the zoophytophagous insect preferably is selected from the subtribe Dicyphina or from the tribe Deraeocorini, and from the subtribe Dicyphina for example is selected from the genus Nesidiocoris, such as Nesidiocoris tenuis, or from the genus Macrolophus, such as Macrolophus pygmaeus, Macrolophus caliginosus, Macrolophus praeclarus or Macrolophus melanotoma or from the genus Engytatus, such as Engytatus modestus, or from the genus Dicyphus, such as Dicyphus tamaninii, Dicyphus hesperus, Dicyphus hyalinipennis, and from the tribe Deraeo
  • a food source comprising protein is provided to the zoophytophagous insect.
  • the proteinaceous food source aids in supporting the protein requirement of the individuals of the zoophytophagous insect population.
  • the protein source may be selected such that it comprises animal protein.
  • Animal protein has a composition well adapted to the requirements of the zoophytophagous insect.
  • the proteinaceous food source is a prey.
  • a prey should be understood to mean a life stage of an animal organism that can be preyed by the zoophytophagous insect.
  • Arthropod eggs such as lepidopteran eggs, more particularly eggs from Ephestia kuehniella or Sitotroga cereallella, or eggs from other insect species such as from medfly (for example as disclosed in WO2011/010308) are particularly suitable prey that may be used as a protein containing food source.
  • Arthropod eggs preferably are provided in a frozen state or dried state.
  • Alternative prey may be selected from Artemia cysts, preferably decapsulated Artemia cysts.
  • a food source comprising a carbohydrate source is also provided to the zoophytophagous insect.
  • the food source comprising a carbohydrate source preferably is an artificial food source, meaning that at least one of its constituents and/or components is in a processed form.
  • the carbohydrate in the carbohydrate source is an isolated carbohydrate, meaning that it is obtained by isolation and/or purification from a natural source.
  • carbohydrate embraces monosaccharides, disaccharides, oligosaccharides and polysaccharides. Within the present invention the use of all these forms of saccharides is envisaged.
  • carbohydrate as a monosaccharide, a disaccharide, a trisaccharide or a tetrasaccahride.
  • Suitable sugars may be selected from glucose, fructose, mannose, sucrose, lactulose, maltose, trehalose, cellobiose, melizitose, trehalulose, glucosucrose, stachyose or mixtures thereof.
  • the carbohydrate may be provided to the zoophytophagous insect in any form which renders it suitable as a food source for the insect. Suitable forms include solid forms and aqueous solutions. Solid forms are preferably provided as powder forms. Solutions, are preferably provided entrapped in a matrix and/or by a coating. The use of an aqueous carbohydrate solution is beneficial in view of the fact that it may also be used as a water source by the zoophytophagous insect.
  • Solutions entrapped in a matrix include gels.
  • Gels may be obtained by using a gelling agent.
  • biological gelling agent is preferred.
  • the selection of biological gelling agent is within the ambit of the knowledge of the skilled person. Examples of suitable biological gelling agents are agar, carrageenan, alginate, gelatine, starch.
  • Solutions entrapped by a coating may be obtained with known encapsulation techniques. Particularly useful are the methods disclosed in US 6,780,507. Products obtained with the methods disclosed herein are obtainable via HYDROCAPSULE ® (Jasper, GA, USA).
  • the concentration of the carbohydrate in the carbohydrate solution may be in the range 0.2 -1.5 M, preferably 0.2-0.8 M, more preferably 0.3-0.6 M.
  • the use of solutions, having a carbohydrate concentration, in particular a sugar concentration, within this range have proven to be well accepted by zoophytophagous insects while obtaining the surprising effects associated with the present invention.
  • the carbohydrate source comprises >15%, >20%, > 25%, > 30%, >35%, >40%, >45%, >50%, >55%, >60%, >65%, >70%, >75%, >80%, >85%, >90%, >95% carbohydrate.
  • the rearing method it is further preferred to provide a water source to the
  • the source of water may for example be plant tissue such as tissue from tobacco plants as used in the rearing method disclosed by Castane and Zapata (2005).
  • Suitable standard conditions are 23-27°C, preferably 25 °C and 70 +/- 10% RH, and a 16:8 (L:D) photoperiod.
  • tobacco plants are used to provide a water source and/or an oviposition substrate.
  • a further aspect of the invention relates to a method for crop protection.
  • a zoophytophagous insect may be suitable for use as a crop protection agent in view of its ability to predate on crop pests.
  • beneficial zoophytophagous predators may also cause damage to crops by feeding on the plant tissue.
  • the inventors of the present invention have found that providing a carbohydrate source on a crop reduces the crop damage caused by a zoophytophagous insect and/or supports the establishement of a population of the zoophytophagous insect in the crop.
  • the method for crop protection comprises:
  • a food source for the zoophytophagous insect, said food source comprising a carbohydrate source.
  • a zoophytophagous insect is provided which is suitable for use as a crop protection agent.
  • the possible selections of zoophytophagous insect and further details concerning the zoophytophagous insect have already been discussed in connection to the preferred and alternative embodiments of the method of rearing.
  • the zoophytophagous insect may be provided on the crop by known methods. Suitable methods may be the methods known for releasing N. tenuis in a crop. In this respect reference may be made to the NESIBUG ® product available from Koppert B. V. (Berkel an Rodenrijs, The Netherlands) and the guidelines for its use.
  • a food source for the zoophytophagous insect is provided on the crop. Said food source comprises a carbohydrate source. Technical details about the food source comprising the carbohydrate source have already been discussed in connection to the method of rearing of the invention.
  • the method for crop protection may be aimed at protection against a pest selected from whiteflies (Hemiptera: Aleyrodidae), Tuta absoluta (Meyrick) (Lepidoptera:
  • a bioactive compound may be any compound which has an effect on the physiology of the zoophytophagous insect.
  • a number of is to be understood to mean one or more.
  • the bioactive compound may have a positive or negative effect on the physiology of the zoophytophagous insect. Positively acting bioactive compounds promote or improve normal physiological conditions and/or functions of the
  • zoophytophagous insect may for example be selected from from natural amino acids, natural proteins, vitamins. Negatively acting bioactive compounds may for example reduce or inhibit normal physiological conditions and/or functions of the zoophytophagous insect. It is preferred that the negatively acting bioactive compounds reduces the population development of the population of the zoophytophagous insect. Bioactive compounds reducing the population development of the zoophytophagous insect may for example be selected from agents that reduce fecundity, or reduce longevity or an insecticide, preferably a biological insecticide. The further provision of a bioactive compounds reducing the population development of the zoophytophagous insect, may be helpful at times where it is beneficial to decrease the number of zoophytophagous insects in the crop.
  • a further aspect of the invention relates to a method for reducing plant damage caused by a zoophytophagous insect.
  • a zoophytophagous insect As already discussed above, both zoophytophagous insects regarded as beneficial and zoophytophagous insects regarded as pests can cause plant damage. According to this aspect of the invention plant damage by a
  • zoophytophagous insect may be reduced by providing on the plant an amount of a carbohydrate source.
  • Yet another aspect of the invention relates to the use of a carbohydrate source as a food source for a zoophytophagous insect.
  • the use of a carbohydrate source as a food source for a zoophytophagous insect has not been disclosed in the prior art, particularly not when used in the presence of plant tissue or prey, which are food source known to be well accepted by zoophytophagous insects.
  • the inventors of the present invention have found that such use of a carbohydrate source as a food source for a zoophytophagous insect results in surprising effects. According to an embodiment of this aspect of the invention the surprising effect is faster establishment of a population of the
  • the surprising effect is reduction of the immature development time and/or increasing the mean body weight of the zoophytophagous insect.
  • Immature development may be measured as the number of days required for development from fresh hatchling (day of hatch from egg) to the last moult (last nymphal stage to adult).
  • the mean body weight may be determined of freshly moulted adults. In view of differences in development of males and females it is preferred to determine the mean body weight per sex.
  • a further aspect of the invention relates to a composition
  • a composition comprising a carbohydrate source for use in reducing the immature development time and/or increasing the mean body weight of a zoophytophagous insect.
  • the composition is used particularly as a food source for a zoophytophagous insect in the presence of a first food source, said first food source being selected from plant tissue or prey, which are food sources known to be well accepted by zoophytophagous insects.
  • Pesticide-free tomato plants (30 cm high) variety "Optima” (from Seminis Vegetable Seeds, Inc., Almeria, Spain) were used in all the assays. Tomato plants were planted on 8 x 8 x 8 cm pots.
  • a climatic chamber 25 ⁇ 1°C, 60 ⁇ 5 % HR, 16:8 h L:D photoperiod
  • N. tenuis was able to obtain the sugars from Hydrocapsules produced by Hydrocapsule Inc. (Jasper, Georgia (USA)) and the effect of their addition on the immature development of N. tenuis when it also feeds on E. kuehniella eggs. These microcapsules have a polymeric shell for the preservation, storage, and controlled delivery of sugars.
  • the Hydrocapsules used in this assay were 1-2 mm in diameter.
  • the core of the Hydrocapsules was 0.5 M sucrose solution (this concentration was selected from the previous results, in prep.).
  • Newly emerged nymphs were individualized in Petri dishes (6 cm in diameter) with a tomato leaf disc and fed with two treatments: E. kuehniella with and without Hydrocapsules. Each treatment was replicated 50 times. The eggs and the tomato leaf disc were added and renewed as above. The hydrocapsules were added ad libitum (0.6 g per dish and day) and renewed daily. The Petri dishes were undisturbed placed in a climatic chamber (25 ⁇ 1°C, 60 ⁇ 5 % HR, 16:8 h L:D photoperiod).
  • the petri dishes were checked daily to determine N. tenuis instar, egg consumption and mortality. These data were used to calculate the nymphal developmental time, the survivorship of each nymphal instar and the number of eggs consumed. Once they reached the adult stage, they were transferred in couples to a plastic cup (370 cm 3 ) with an apical shoot of tomato (14 cm approximately) with E. kuehniella eggs (300-400 eggs) and Hydrocapsules (0.6 g) ad libitum. To maintain the shoot turgor, the plastic cup was introduced in a smaller plastic glass (230 cm 3 ) with water. Tomato shoots reached the water through a hole in the base of the first glass (Sanchez et al. 2009). The glass with tomato and N.
  • N. tenuis was used as a model organism. The number of necrotic rings per plant was counted in plants with and without sugars added. Nesidiocoris tenuis produces necrotic rings when it feeds on tomato plants (Calvo et al. 2009, Arno et al. 2010a, Sanchez and Lacasa 2008). This parameter has been used to evaluate the damages produced by N. tenuis under field conditions (Calvo and Urbaneja 2004). This assay was conducted in a greenhouse at Instituto Valenciano de Investigaations Agrarias (IVIA) (Montcada, Valencia). The environmental conditions were 25 ⁇ 2 °C, 65 ⁇ 10% RH and natural photoperiod.
  • sugar sources Two amounts of sugar sources (one or three eppendorfs per plant) and a control (without sugar addition) were compared. Sugar sources were placed in the apical part of the tomato plant. Eppendorfs were filled with 0.5 M sucrose solution and tapped with cotton as in the previous assay.
  • Each replicate consisted of a plastic cage (55 x 30 x 30 cm) with two lateral holes and one in the upper part (10 X 10 cm) sealed with muslin to permit ventilation.
  • One of the lateral holes had a muslin sleeve to manipulate the interior part of the cage.
  • a tomato plant with the eppendorfs (0, 1 or 3 depending on the treatment) and a couple of N. tenuis (3-days old). Every two days, the plants were watered and the eppendorfs renewed. Each treatment was replicated 16 times (a total of 48 cages). Seven days later, eight plants of each treatment were taken to the lab. Once there, the number of necrotic rings and N.
  • Macrolophus pygmaeus has been mass-reared for several generations (>100) at the Koppert plant.
  • the system supplies frozen Ephestia eggs as a food source and plant material as moisture source and oviposition substrate (replaced twice a week).
  • Fresh nymphs (0-3 days old) were collected at the beginning of the trial from this mass- rearing.
  • Ephestia was offered on sticky labels (12x18 mm), to all rearing units. Each label prepared in this way contained an amount of ⁇ 40 mg.
  • Fecundity of Macrolophus pygmaeus was assessed according to a direct method described by Callebaut et al (2004).
  • the diet consisted either of pure frozen Ephestia kuehniella eggs or the same eggs supplemented with powdered saccharose sugar, both in combination with plant material as a moisture source and oviposition substrate. After 1 week of maturation the adults were dissected and the resulting egg counts provide a prediction of life-time fecundity.
  • Fresh nymphs (0-3 days old) were collected from this mass-rearing and reared until adulthood in the laboratory according the control in experiment 4. This experiment was started with fresh adults (0-3 days) and the experiment was performed in a petri-dish (0 7,5 cm,h 3 cm) with a ventilated gauze lid. 10-15 adults of both sexes were placed in each unit on day 0, together with the treatment diet and plant material as a moisture source and oviposition substrate. Ephestia was offered on 2 sticky labels (prepared as in trial 1), to all rearing units and replaced twice a week. Powdered sugar was obtained from retail (Van Gilse) and contained 99.2 % sucrose and E341. The treatments were as follows: Ephestia eggs + powdered saccharose sugar (50:50 b/w), and Ephestia eggs.
  • Phtyophagy in predaceaous Heteroptera effects on life history and population dynamics. Phtyophagy in predaceaous Heteroptera:

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Animal Behavior & Ethology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention appartient au domaine de la protection biologique des récoltes. Selon un premier aspect, l'invention concerne un procédé d'élevage d'un insecte zoophytophage. D'autres aspects de l'invention concernent un procédé de protection des récoltes, un procédé de réduction des dommages causés aux récoltes par un insecte zoophytophage, l'utilisation d'une source d'hydrates de carbone en tant que source de nutrition pour un insecte zoophytophage, des compositions comportant une source d'hydrates de carbone destinée à des usages particuliers en relation avec le développement d'un insecte zoophytophage et l'utilisation d'une source d'hydrates de carbone en tant que source d'alimentation pour un insecte zoophytophage.
PCT/NL2012/050683 2011-09-29 2012-09-28 Procédés et usages améliorés liés à l'élevage d'un insecte zoophytophage et protection des récoltes WO2013048251A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1039084A NL1039084C2 (en) 2011-09-29 2011-09-29 Improved methods and uses related to rearing of a zoophytophagous insect and crop protection.
NL1039084 2011-09-29

Publications (1)

Publication Number Publication Date
WO2013048251A1 true WO2013048251A1 (fr) 2013-04-04

Family

ID=47116211

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2012/050683 WO2013048251A1 (fr) 2011-09-29 2012-09-28 Procédés et usages améliorés liés à l'élevage d'un insecte zoophytophage et protection des récoltes

Country Status (2)

Country Link
NL (1) NL1039084C2 (fr)
WO (1) WO2013048251A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016023957A1 (fr) * 2014-08-12 2016-02-18 Invivo Agrosolutions Composition comprenant des oeufs d'arthropodes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999063814A1 (fr) 1998-06-08 1999-12-16 The United States Of America, Represented By The Secretary Of Agriculture Milieu artificiel pour l'elevage d'entomophages
WO2000049897A1 (fr) 1999-02-25 2000-08-31 The United States Of America, As Represented By The Secretary Of Agriculture Milieux nutritifs artificiels pour arthropodes
US6780507B2 (en) 2000-02-09 2004-08-24 Analytical Research Systems, Inc. Hydrocapsules and method of preparation thereof
WO2011010308A2 (fr) 2009-07-23 2011-01-27 Bio-Fly Ltd Système et procédé pour nourrir des insectes bénéfiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999063814A1 (fr) 1998-06-08 1999-12-16 The United States Of America, Represented By The Secretary Of Agriculture Milieu artificiel pour l'elevage d'entomophages
WO2000049897A1 (fr) 1999-02-25 2000-08-31 The United States Of America, As Represented By The Secretary Of Agriculture Milieux nutritifs artificiels pour arthropodes
US6780507B2 (en) 2000-02-09 2004-08-24 Analytical Research Systems, Inc. Hydrocapsules and method of preparation thereof
WO2011010308A2 (fr) 2009-07-23 2011-01-27 Bio-Fly Ltd Système et procédé pour nourrir des insectes bénéfiques

Non-Patent Citations (25)

* Cited by examiner, † Cited by third party
Title
ARNO, J.; C. CASTANE; J. RIUDAVETS; R. GABARRA.: "Risk of damage to tomato crops by the generalist zoophytophagous predator Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae", BULL. ENTOMOL. RES., vol. 100, 2010, pages 105 - 115
ARNO, J.; R. GABARRA; T.-X. LIU; A. M. SIMMONS; D. GERLING: "Bemisia: Bionomics and Management of a Global Pest.", 2010, SPRINGER, article "Natural Enemies of Bemisia tabaci: Predators and Parasitoids", pages: 385 - 421
CALLEBAUT, E.; VAN BAAL, B.; VANDEKERKHOVE, K.; BOLCKMANS, P.; DE CLERCQ: "A fecundity test for assessing the quality of Macrolophus caliginosus reared on artificial diets", PARASITICA, vol. 60, no. 1-2, 2004, pages 9 - 14
CALVO, F. J.; A. URBANEJA: "Nesidiocoris tenuis un aliado para el control biologico de mosca blanca", HORTICULTURA INTERNACIONAL, vol. 44, 2004, pages 20 - 25
CALVO, J.; J. E. BELDA; A. GIMENEZ.: "Una nueva estrategia para el control biológico de mosca blanca y Tuta absoluta en tomate", PHYTOMA ESPANA, vol. 216, 2010, pages 46 - 52
CALVO, J.; K. BOLCKMANS; P. A. STANSLY; A. URBANEJA: "Predation by Nesidiocoris tenuis on Bemisia tabaci and injury to tomato", BIOCONTROL, vol. 54, 2009, pages 237 - 246, XP019670167
CASTAÑÉ, C.; J. ARNO; F. BEITIA; R. GABARRA.: "Control biológico de plagas agricolas", 2008, article "Control biológico de moscas blancas", pages: 239 - 253
CASTAÑÉ, C.; ZAPATA, R.: "Rearing the predatory bug Macrolophus caliginosus on a meat-based diet", BIOLOGICAL CONTROL, vol. 34, 2005, pages 66 - 72, XP004918407, DOI: doi:10.1016/j.biocontrol.2005.04.002
COHEN, A.C.: "Insect Diets, science and technology", 2004, CRC PRESS, pages: 324
DESNEUX, N.; E. WAJNBERG; K. WYCKHUYS; G. BURGIO; S. ARPAIA; C. NARVAEZ-VASQUEZ; J. GONZÁLEZ-CABRERA; D. CATALÁN-RUESCAS; E. TAB: "Biological invasion of European tomato crops by Tuta absoluta: Ecology, geographic expansion and prospects for biological control", J. PEST SCIENCE, vol. 83, 2010, pages 197 - 215, XP019810369
GABARRA, R.; J. ARNO; J. RIUDAVETS: "Control biológico de plagas agricolas", 2008, article "Tomate", pages: 410 - 422
GILLESPIE, D. R.; R. R. MCGREGOR: "The functions of plant feeding in the omnivorous predator Dicyphus hesperus: water places limits on predation", ECOL. ENTOMOL., vol. 25, 2000, pages 380 - 386
GONZÁLEZ-CABRERA, J.; O. MOLLA; H. MONTON; A. URBANEJA.: "Efficacy of Bacillus thuringiensis (Berliner) for controlling the tomato borer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae", BIOCONTROL, vol. 56, 2011, pages 71 - 80
JACAS, J. A.; A. URBANEJA; F. GARCIA-MARI.: "Control biológico de plagas agricolas", 2008, article "Artropodos depredadores", pages: 39 - 56
KOPPERT B.S., EL CONTROL BIOLÓGICO EN TOMATE: UNA REALIDAD PROBADA, 2011, Retrieved from the Internet <URL:http://www. koppert. es/noticias/control-biologico/detalle/el-control-biologico-en-tomate-una- realidad-probada>
MARTINEZ-CASCALES, J.I.; CENIS, J.L.; CASSIS, G.; SANCHEZ., J.A.: "Species identity of Macrolophus melanotoma (Costa 1853) and Macrolophus pygmaeus (Rambur 1839) (Insecta: Heteroptera: Miridae) based on morpholigical and molecular data and binomic implications", INSECT SYSTEMATICS AND EVOLUTION, vol. 35, no. 4, 2006, pages 385 - 404
MOLLÁ, 0.; J. GONZÁLEZ-CABRERA; A. URBANEJA.: "The combined use of Bacillus thuringiensis and Nesidiocoris tenuis against the tomato borer Tuta absoluta", BIOCONTROL IN PRESS, 2011
MONSERRAT, A.: "Medidas basicas para el manejo de la polilla del tomate Tuta absoluta", AGRICOLA VERGEL, vol. 333, 2009, pages 481 - 491
NARANJO, S.E; GIBSON, R.L.: "Phytophagy in predaceous Heteroptera: effects on life history and population dynamics. Phtyophagy in predaceaous Heteroptera: Zoophytophagous Heteroptera", THOMAS SAY PUBLICATIONS IN ENTOMOLOGY, 1996, pages 57 - 93
SANCHEZ, J. A.: "Zoophytophagy in the plantbug Nesidiocoris tenuis", AGRICULTURAL AND FOREST ENTOMOLOGY, vol. 10, 2008, pages 75 - 80
SANCHEZ, J. A.; A. LACASA.: "Impact of the zoophytophagous plant bug Nesidiocoris tenuis (Heteroptera: Miridae) on tomato yield", J. ECON. ENTOMOL., vol. 101, 2008, pages 1864 - 1870
SANCHEZ, J. A.; A. LACASA; J. ARNO; C. CASTANE; O. ALOMAR: "Life history parameters for Nesidiocoris tenuis (Reuter) (Het., Miridae) under different temperature regimes", J. APPL. ENTOMOL., vol. 133, 2009, pages 125 - 132
SCHUH, R.T.; SLATER, J.A.: "True bugs of the world (Hemiptera: Heteroptera): classification and natural history. London", 1995, COMSTOCK PUBLISHING ASSOCIATES, pages: 366
SEAGRAVES, M. P.; Y. KAJITA; D. C. WEBER; J. J. OBRYCKI; J. G. LUNDGREN.: "Sugar feeding by coccinellids under field conditions: the effects of sugar sprays in soybean", BIOCONTROL, vol. 56, 2011, pages 305 - 314, XP019907639, DOI: doi:10.1007/s10526-010-9337-3
URBANEJA, A.; G. TAPIA; P. STANSLY: "Influence of host plant and prey availability on developmental time and surviorship ofNesidiocoris tenius (Het.: Miridae", BIOCONTROL SCI. TECHNOL., vol. 15, 2005, pages 513 - 518

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016023957A1 (fr) * 2014-08-12 2016-02-18 Invivo Agrosolutions Composition comprenant des oeufs d'arthropodes
FR3024820A1 (fr) * 2014-08-12 2016-02-19 Invivo Agrosolutions Composition comprenant des oeufs d'arthropodes

Also Published As

Publication number Publication date
NL1039084C2 (en) 2013-04-02

Similar Documents

Publication Publication Date Title
De Clercq et al. Production of heteropteran predators
Kaya et al. Encapsulation of steinernematid and heterorhabditid nematodes with calcium alginate: a new approach for insect control and other applications
Delisle et al. Evaluation of various types of supplemental food for two species of predatory mites, Amblyseius swirskii and Neoseiulus cucumeris (Acari: Phytoseiidae)
Urbaneja-Bernat et al. Sugar as nutritional supplement for the zoophytophagous predator Nesidiocoris tenuis
Arijs et al. Rearing Orius laevigatus on cysts of the brine shrimp Artemia franciscana
Urbaneja-Bernat et al. Reduced phytophagy in sugar-provisioned mirids
Resende et al. Flowers of Apiaceous species as sources of pollen for adults of Chrysoperla externa (Hagen)(Neuroptera)
Sétamou et al. Bionomics of Mussidia nigrivenella (Lepidoptera: Pyralidae) on three host plants
Ndemah et al. The role of wild grasses in the management of lepidopterous stem-borers on maize in the humid tropics of western Africa
Akter et al. Biology of the Angoumois grain moth, Sitotroga cerealella (Oliver) on stored rice grain in laboratory condition
Showler Effects of compost and chicken litter on soil nutrition, and sugarcane physiochemistry, yield, and injury caused by Mexican rice borer, Eoreuma loftini (Dyar)(Lepidoptera: Crambidae)
CN113317091A (zh) 利用斜纹夜蛾繁育草地贪夜蛾优势天敌甲腹茧蜂的方法
Pereira et al. Silicon supplementation of maize impacts fall armyworm colonization and increases predator attraction
Romeis et al. Nectar-and pollen-feeding by adult herbivorous insects
Dindo et al. Production of dipteran parasitoids
Mátray et al. Do floral resources affect fitness of adult Cydia pomonella (Linnaeus 1758)(Lepidoptera: Tortricidae)?
NL1039084C2 (en) Improved methods and uses related to rearing of a zoophytophagous insect and crop protection.
Showler et al. Transgenic Bt corn varietal resistance against the Mexican rice borer, Eoreuma loftini (Dyar)(Lepidoptera: Crambidae) and implications to sugarcane
Marchioro et al. Importance of carbohydrate sources to the reproductive output of the wheat armyworm Pseudaletia sequax
Gündüz et al. Effects of sugar feeding on lipid, glycogen, and total sugar levels of a female parasitoid, Bracon hebetor (Say)(Hymenoptera: Braconidae)
Zaché et al. Evaluation of Palmistichus elaeisis Delvare & LaSalle (Hymenoptera: Eulophidae) as parasitoid of the Sarsina violascens Herrich-Schaeffer (Lepidoptera: Lymantriidae)
Santos Alternatives to chemical control of stored-product insects on small farms in the tropics.
Bouslama et al. Observations of some biological characteristics of Helicoverpa armigera reared under controlled conditions.
Lundbye et al. The effect of a seed-sucking bug on seed germination of an arctic cushion plant
Bhardwaj DEPARTMENT OF ENTOMOLOGY COLLEGE OF AGRICULTURE FACULTY OF AGRICULTURE INDIRA GANDHI KRISHI VISHWAVIDYALAYA RAIPUR (Chhattisgarh)

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12780302

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12780302

Country of ref document: EP

Kind code of ref document: A1