WO2013033094A3 - Elimination of seed formation by ablating floral organs - Google Patents

Elimination of seed formation by ablating floral organs Download PDF

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Publication number
WO2013033094A3
WO2013033094A3 PCT/US2012/052662 US2012052662W WO2013033094A3 WO 2013033094 A3 WO2013033094 A3 WO 2013033094A3 US 2012052662 W US2012052662 W US 2012052662W WO 2013033094 A3 WO2013033094 A3 WO 2013033094A3
Authority
WO
WIPO (PCT)
Prior art keywords
ablating
elimination
plants
seed formation
floral organs
Prior art date
Application number
PCT/US2012/052662
Other languages
French (fr)
Other versions
WO2013033094A2 (en
Inventor
Chunsheng Zhang
Kimberly Ann Winkeler
Kim Norris-Caneda
Original Assignee
Arborgen Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arborgen Inc. filed Critical Arborgen Inc.
Publication of WO2013033094A2 publication Critical patent/WO2013033094A2/en
Publication of WO2013033094A3 publication Critical patent/WO2013033094A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/829—Female sterility

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to methods to limit or eliminate migration of pollen and seeds from transgenic plants into wild plant populations by producing sterile transgenic plants capable of achieving full vegetative growth. Specifically, the present invention provides targeted ablation of reproductive structures in plants by devising a promoter specific for carpel tissue and transforming plants with DNA constructs in which the promoter mediates expression of cytotoxic genes.
PCT/US2012/052662 2011-08-31 2012-08-28 Elimination of seed formation by ablating floral organs WO2013033094A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161529595P 2011-08-31 2011-08-31
US61/529,595 2011-08-31

Publications (2)

Publication Number Publication Date
WO2013033094A2 WO2013033094A2 (en) 2013-03-07
WO2013033094A3 true WO2013033094A3 (en) 2014-05-08

Family

ID=47757147

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/052662 WO2013033094A2 (en) 2011-08-31 2012-08-28 Elimination of seed formation by ablating floral organs

Country Status (1)

Country Link
WO (1) WO2013033094A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015294830A1 (en) * 2014-08-01 2017-02-02 The New Zealand Institute For Plant And Food Research Limited Methods and materials for producing coreless fruit
CN111454955B (en) * 2020-04-27 2023-05-12 广西壮族自治区林业科学研究院 RNAi fragment derived from Eucalyptus urophylla CAD gene sequence and application thereof
CN117417930A (en) * 2023-09-26 2024-01-19 南京林业大学 Segmented genome of populus nigra lncRNA, segmentation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090075326A1 (en) * 2004-09-22 2009-03-19 Arborgen, Llc Reproductive ablation constructs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090075326A1 (en) * 2004-09-22 2009-03-19 Arborgen, Llc Reproductive ablation constructs

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 16 April 2009 (2009-04-16), "Eucalyptus camaldulensis AGL gene for agamous-like protein, promoter region, clone: EcAGL1-pro", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/AB465732.1 accession no. B465732.1 *
KATO ET AL.: "Isolation and expression analysis of AGAMOUS-like genes from Eucalyptus grandis.", PLANT BIOTECHNOLOGY, vol. 26, no. 1, 25 March 2009 (2009-03-25), pages 121 - 124 *
YANG ET AL.: "Two similar but distinct second intron fragments from tobacco AGAMOUS homologs confer identical floral organ-specific expression sufficient for generating complete sterility in plants.", PLANTA, vol. 231, no. 5, April 2010 (2010-04-01), pages 1159 - 1169, XP019803074 *

Also Published As

Publication number Publication date
WO2013033094A2 (en) 2013-03-07

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