WO2013028058A1 - Appareil pour le tri de particules par diélectrophorèse - Google Patents

Appareil pour le tri de particules par diélectrophorèse Download PDF

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Publication number
WO2013028058A1
WO2013028058A1 PCT/MY2012/000168 MY2012000168W WO2013028058A1 WO 2013028058 A1 WO2013028058 A1 WO 2013028058A1 MY 2012000168 W MY2012000168 W MY 2012000168W WO 2013028058 A1 WO2013028058 A1 WO 2013028058A1
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WO
WIPO (PCT)
Prior art keywords
electrode
microchannel
electrodes
primary
particles
Prior art date
Application number
PCT/MY2012/000168
Other languages
English (en)
Inventor
Hing Wah Lee
Ismahadi Syono MOHD
Bin Sulaiman Azhar
Original Assignee
Mimos Berhad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mimos Berhad filed Critical Mimos Berhad
Publication of WO2013028058A1 publication Critical patent/WO2013028058A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications

Definitions

  • the present invention relates to an apparatus for sorting particles by dielectrophoresis in a microfluidic system.
  • DEP Dielectrophoresis
  • Patent Publication No. 2006/0177815 which discloses methods, devices and systems that utilize dielectrophoretic forces to separate a target species from a plurality of species in a sample. Another example of such apparatus is provided in US Patent Publication No.
  • the microfluidic dielectrophoresis separating device includes a primary passage, at least a secondary passage and at least an electrode assembly.
  • the primary passage has a primary flow containing a plurality of particulates flowing therein.
  • the secondary passage has an input path and an output path and is connected with the primary passage.
  • the electrode assembly generates a dielectrophoresis force to drive a specific one of the particulates into the output path.
  • the present invention provides an apparatus for sorting particles by dielectrophoresis.
  • the apparatus comprises of a primary microchannel (110), at least one side microchannel (120), and at least three electrically coupled electrodes (131 , 132, 133).
  • the electrodes (131 , 132, 133) are arranged in the primary microchannel (110) and are positioned near the proximal end of the side microchannel (120), wherein a first electrode (131) is a planar electrode forming a part of the bottom inner surface of the primary microchannel (110), and wherein a second electrode (132) is a planar electrode forming a part of the top inner surface of the primary microchannel (110), and wherein a third electrode (133) is a comb-like electrode suspended in the middle of the primary microchannel (110) and planarly inclined towards the side microchannel (120).
  • the electrodes (131 , 132, 133) are placed vertically opposite one another, wherein the first electrode (131) is placed directly below the third electrode (133) while the second electrode (132) is placed directly above the third electrode (133).
  • the electrodes (131, 132, 133) are connected to one or more power source of either DC or AC voltage, and wherein the first and second electrodes (131, 132) have an opposite polarity from the third electrode (133).
  • FIG. 1 illustrates a top view of an apparatus for sorting particles by dieletrophoresis according to an embodiment of the present invention.
  • FIG. 2 illustrates a cross sectional view of the apparatus of FIG. 1.
  • FIGS. 3(a-d) illustrate an operation of the apparatus of FIG. 1 according to an embodiment of the present invention. DESCRIPTION OF THE PREFFERED EMBODIMENT
  • the apparatus generally comprises of a primary microchannel (110) having a side microchannel (120) and three electrically coupled electrodes (131, 132, 133).
  • the apparatus may comprise additionally one or more side microchannels (120) that are capable of flowing fluid and sorted particles from the primary microchannel (110) to a side outlet (121).
  • the primary microchannel (110) is used to flow fluid containing particles from its inlet (111) to its outlet (112), while the side microchannel (120) is used to flow particles sorted from the fluid flowing through the primary microchannel (110) to a side outlet (121).
  • the primary channel (110) is suitably made of silicon, glass or polymers such as, but not limited to polydimethylsiloxane (PDMS), poly-methyl- metha-crylate (PMMA) or polyimide.
  • the three electrodes (131, 132, 133) are arranged in the primary microchannel (110) and are positioned near the proximal end of the side microchannel (120) so as to deflect selected particles from the fluid into the side channel (120) and onto distal end of the side microchannel (120).
  • a first electrode (131) is a planar electrode forming a part of the bottom inner surface of the primary microchannel (110).
  • a second electrode (132) is a planar electrode forming a part of the top inner surface of the primary microchannel (110).
  • a third electrode (133) is a comb-like electrode suspended in the middle of the primary microchannel (110). Moreover, the third electrode (133) is planarly inclined towards the side microchannel (120).
  • the electrodes (131 , 132, 133) are placed vertically opposite one another, wherein the first electrode (131) is placed directly below the third electrode (133) while the second electrode (132) is placed directly above the third electrode (133).
  • the electrodes (131, 132, 133) are made out of conducting materials such as, but not limited to doped polysilicon, metals or conductive polymers.
  • the electrodes (131, 132, 133) are connected to one or more power source of either DC or AC voltage.
  • the first and second electrodes (131 , 132) have an opposite polarity from the third electrode (133).
  • an electric field is generated in between the first and third electrodes (131 , 133) and another electric field is generated in between the second and third electrodes (132, 133) when voltage is applied to the electrodes (131 , 132, 133).
  • particles having a particular properties or sizes can be sort out from a fluid flowing through the primary microchannel (110).
  • FIGS. 3 a method of sorting particles by using the apparatus of FIG. 1 is illustrated.
  • a fluid is flowed through the primary microchannel (110) as shown in FIG. 3a.
  • Voltage is applied to the electrodes, wherein the first and second electrodes (131, 132) are applied with electrical potential having polarity opposite of those from the third electrode (133).
  • an electric field is generated in between the first and third electrodes (131, 133) and in between the second and third electrodes (132, 133).
  • the particles (10) of the fluid are subjected to a non-uniform electric field that creates a DEP force onto selected particles (10a) of the fluid.
  • the DEP force levitates and attracts the selected particles (10a) towards the third electrodes (133) as shown in FIG. 3b.
  • the DEP force blocks the progress of the selected particles (10a) through the primary microchannel (110) while allowing non-selected particles (10b) to pass through to the outlet (112) of the primary microchannel (110).
  • the hydraulic force from the fluid flow continues to push the particles (10) forward and thus, a combination of the DEP force and the hydraulic force generate a net force in the direction along the third electrode (133) as shown in FIG. 3c.
  • the selected particles (10a) are deflected into the side microchannel (120) and on to the outlet (121) of the side microchannel (120) as shown in FIG. 3d.
  • the selected particles (10a) may flow through the outlet (112) of the primary microchannel (110).
  • the comb-like structure of the third electrode (133) creates a multiple electric field with the first and second electrodes (131 , 132) and thereby, the selected particles (10a) that escaped from the initial DEP force is subsequently exposed to another electric field. Thereon, the sorted particles (10a) can be deflected towards the side microchannel (120).

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  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

La présente invention concerne un appareil pour le tri de particules par diélectrophorèse. L'appareil comporte un micro-canal primaire (110), au moins un micro-canal latéral (120), et au moins trois électrodes couplées électriquement (131, 132, 133). Les électrodes (131, 132, 133) sont disposées dans le micro-canal primaire (110) et sont positionnées à proximité de l'extrémité proximale du micro-canal latéral (120). Une première électrode (131) est une électrode planaire formant une partie de la surface intérieure inférieure du micro-canal primaire (110). Une seconde électrode (132) est une électrode planaire formant une partie de la surface intérieure supérieure du micro-canal primaire (110). Une troisième électrode (133) est une électrode de type peigne suspendue au milieu du micro-canal primaire (110) en inclinaison planaire vers le micro-canal latéral (120).
PCT/MY2012/000168 2011-08-24 2012-06-29 Appareil pour le tri de particules par diélectrophorèse WO2013028058A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2011700133A MY179484A (en) 2011-08-24 2011-08-24 Apparatus for sorting particles by dielectrophoresis
MYPI2011700133 2011-08-24

Publications (1)

Publication Number Publication Date
WO2013028058A1 true WO2013028058A1 (fr) 2013-02-28

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PCT/MY2012/000168 WO2013028058A1 (fr) 2011-08-24 2012-06-29 Appareil pour le tri de particules par diélectrophorèse

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MY (1) MY179484A (fr)
WO (1) WO2013028058A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150041325A1 (en) * 2012-03-27 2015-02-12 The Regents Of The University Of California Continuous whole-chip 3-dimensional dep cell sorter and related fabrication method

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6663757B1 (en) * 1998-12-22 2003-12-16 Evotec Technologies Gmbh Method and device for the convective movement of liquids in microsystems
US20060024802A1 (en) * 2002-11-29 2006-02-02 Evotec Oai Ag Fluidic microsystem comprising field-forming passivation layers provided on microelectrodes
US20060177815A1 (en) 2004-11-29 2006-08-10 The Regents Of The University Of California Dielectrophoretic particle sorter
US20060290745A1 (en) * 2005-06-27 2006-12-28 Cfd Research Corporation Method and apparatus for separating particles by dielectrophoresis
EP1764418A1 (fr) * 2005-09-14 2007-03-21 STMicroelectronics S.r.l. Procédé et dispositif pour le traitement d'échantillons biologiques par la diélectrophorèse
US20070131554A1 (en) 2005-12-09 2007-06-14 Industrial Technology Research Institute Multi-sample microfluidic dielectrophoresis separating device and method thereof
US20070273356A1 (en) * 2004-08-31 2007-11-29 Niigata University Method for Electrically Detecting Motion of Nonpolar Composite Molecule by Utilizing Nonuniform Electric Field
US20090288963A1 (en) * 2006-05-31 2009-11-26 Mindseed Laboratories S.R.L. Method and apparatus for manipulating single cells and small aggregates thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6663757B1 (en) * 1998-12-22 2003-12-16 Evotec Technologies Gmbh Method and device for the convective movement of liquids in microsystems
US20060024802A1 (en) * 2002-11-29 2006-02-02 Evotec Oai Ag Fluidic microsystem comprising field-forming passivation layers provided on microelectrodes
US20070273356A1 (en) * 2004-08-31 2007-11-29 Niigata University Method for Electrically Detecting Motion of Nonpolar Composite Molecule by Utilizing Nonuniform Electric Field
US20060177815A1 (en) 2004-11-29 2006-08-10 The Regents Of The University Of California Dielectrophoretic particle sorter
US20060290745A1 (en) * 2005-06-27 2006-12-28 Cfd Research Corporation Method and apparatus for separating particles by dielectrophoresis
EP1764418A1 (fr) * 2005-09-14 2007-03-21 STMicroelectronics S.r.l. Procédé et dispositif pour le traitement d'échantillons biologiques par la diélectrophorèse
US20070131554A1 (en) 2005-12-09 2007-06-14 Industrial Technology Research Institute Multi-sample microfluidic dielectrophoresis separating device and method thereof
US20090288963A1 (en) * 2006-05-31 2009-11-26 Mindseed Laboratories S.R.L. Method and apparatus for manipulating single cells and small aggregates thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150041325A1 (en) * 2012-03-27 2015-02-12 The Regents Of The University Of California Continuous whole-chip 3-dimensional dep cell sorter and related fabrication method
US9770721B2 (en) * 2012-03-27 2017-09-26 The Regents Of The University Of California Continuous whole-chip 3-dimensional DEP cell sorter and related fabrication method
US10967387B2 (en) 2012-03-27 2021-04-06 The Regents Of The University Of California Continuous whole-chip 3-dimensional DEP cell sorter and related fabrication method

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Publication number Publication date
MY179484A (en) 2020-11-08

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