WO2012178101A3 - Compositions and methods to remove genetic markers using counter-selection - Google Patents
Compositions and methods to remove genetic markers using counter-selection Download PDFInfo
- Publication number
- WO2012178101A3 WO2012178101A3 PCT/US2012/043868 US2012043868W WO2012178101A3 WO 2012178101 A3 WO2012178101 A3 WO 2012178101A3 US 2012043868 W US2012043868 W US 2012043868W WO 2012178101 A3 WO2012178101 A3 WO 2012178101A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- compositions
- counter
- selection
- genetic markers
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present disclosure provides compositions and methods for genetic marker removal in cyanobacteria. Cyanobacteria are developed having a genetic marker capable of being selected against in the presence of a negatively selective condition, resulting in removal of a positively selective marker (e.g., an antibiotic resistance marker) from the host cell.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161500528P | 2011-06-23 | 2011-06-23 | |
US61/500,528 | 2011-06-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2012178101A2 WO2012178101A2 (en) | 2012-12-27 |
WO2012178101A3 true WO2012178101A3 (en) | 2013-04-04 |
Family
ID=47423246
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2012/043868 WO2012178101A2 (en) | 2011-06-23 | 2012-06-22 | Compositions and methods to remove genetic markers using counter-selection |
Country Status (1)
Country | Link |
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WO (1) | WO2012178101A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9447422B2 (en) | 2012-12-06 | 2016-09-20 | Synthetic Genomics, Inc. | Autonomous replication sequences and episomal DNA molecules |
CN112300973B (en) * | 2019-08-02 | 2022-06-24 | 南京理工大学 | Rhodococcus gene editing method using phenylalanyl-tRNA synthetase gene mutant as reverse screening marker |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090181434A1 (en) * | 2008-01-03 | 2009-07-16 | Proterro, Inc. | Transgenic photosynthetic microorganisms and photobioreactor |
US20110053273A1 (en) * | 2007-10-08 | 2011-03-03 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
US20110053216A1 (en) * | 2006-10-20 | 2011-03-03 | Vermaas Willem F J | Modified Cyanobacteria |
-
2012
- 2012-06-22 WO PCT/US2012/043868 patent/WO2012178101A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110053216A1 (en) * | 2006-10-20 | 2011-03-03 | Vermaas Willem F J | Modified Cyanobacteria |
US20110053273A1 (en) * | 2007-10-08 | 2011-03-03 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
US20090181434A1 (en) * | 2008-01-03 | 2009-07-16 | Proterro, Inc. | Transgenic photosynthetic microorganisms and photobioreactor |
Non-Patent Citations (2)
Title |
---|
DATABASE GENBANK 14 March 2008 (2008-03-14), "Pyrimidine operon attenuation protein/uracil phosphoribosyltransferase", retrieved from http://www.ncbi.nlm.nih.govlprotein/ACA99681 accession no. CA99681 * |
DATABASE GENBANK 14 March 2008 (2008-03-14), "Synechococcus sp. PCC 7002, complete genome", retrieved from http://www.ncbi.nlm.nih.gov/nucleotide/CP000951 accession no. P000951 * |
Also Published As
Publication number | Publication date |
---|---|
WO2012178101A2 (en) | 2012-12-27 |
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