WO2012178101A3 - Compositions and methods to remove genetic markers using counter-selection - Google Patents

Compositions and methods to remove genetic markers using counter-selection Download PDF

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Publication number
WO2012178101A3
WO2012178101A3 PCT/US2012/043868 US2012043868W WO2012178101A3 WO 2012178101 A3 WO2012178101 A3 WO 2012178101A3 US 2012043868 W US2012043868 W US 2012043868W WO 2012178101 A3 WO2012178101 A3 WO 2012178101A3
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WO
WIPO (PCT)
Prior art keywords
methods
compositions
counter
selection
genetic markers
Prior art date
Application number
PCT/US2012/043868
Other languages
French (fr)
Other versions
WO2012178101A2 (en
Inventor
Yu Xu
Brian D. Green
Original Assignee
Joule Unlimited Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joule Unlimited Technologies, Inc. filed Critical Joule Unlimited Technologies, Inc.
Publication of WO2012178101A2 publication Critical patent/WO2012178101A2/en
Publication of WO2012178101A3 publication Critical patent/WO2012178101A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure provides compositions and methods for genetic marker removal in cyanobacteria. Cyanobacteria are developed having a genetic marker capable of being selected against in the presence of a negatively selective condition, resulting in removal of a positively selective marker (e.g., an antibiotic resistance marker) from the host cell.
PCT/US2012/043868 2011-06-23 2012-06-22 Compositions and methods to remove genetic markers using counter-selection WO2012178101A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161500528P 2011-06-23 2011-06-23
US61/500,528 2011-06-23

Publications (2)

Publication Number Publication Date
WO2012178101A2 WO2012178101A2 (en) 2012-12-27
WO2012178101A3 true WO2012178101A3 (en) 2013-04-04

Family

ID=47423246

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/043868 WO2012178101A2 (en) 2011-06-23 2012-06-22 Compositions and methods to remove genetic markers using counter-selection

Country Status (1)

Country Link
WO (1) WO2012178101A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9447422B2 (en) 2012-12-06 2016-09-20 Synthetic Genomics, Inc. Autonomous replication sequences and episomal DNA molecules
CN112300973B (en) * 2019-08-02 2022-06-24 南京理工大学 Rhodococcus gene editing method using phenylalanyl-tRNA synthetase gene mutant as reverse screening marker

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090181434A1 (en) * 2008-01-03 2009-07-16 Proterro, Inc. Transgenic photosynthetic microorganisms and photobioreactor
US20110053273A1 (en) * 2007-10-08 2011-03-03 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US20110053216A1 (en) * 2006-10-20 2011-03-03 Vermaas Willem F J Modified Cyanobacteria

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110053216A1 (en) * 2006-10-20 2011-03-03 Vermaas Willem F J Modified Cyanobacteria
US20110053273A1 (en) * 2007-10-08 2011-03-03 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US20090181434A1 (en) * 2008-01-03 2009-07-16 Proterro, Inc. Transgenic photosynthetic microorganisms and photobioreactor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 14 March 2008 (2008-03-14), "Pyrimidine operon attenuation protein/uracil phosphoribosyltransferase", retrieved from http://www.ncbi.nlm.nih.govlprotein/ACA99681 accession no. CA99681 *
DATABASE GENBANK 14 March 2008 (2008-03-14), "Synechococcus sp. PCC 7002, complete genome", retrieved from http://www.ncbi.nlm.nih.gov/nucleotide/CP000951 accession no. P000951 *

Also Published As

Publication number Publication date
WO2012178101A2 (en) 2012-12-27

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