WO2012167338A3 - Processo para a obtenção de um substrato de cultivo para células-tronco pluripotentes e substrato de cultivo produzido pelo mesmo - Google Patents

Processo para a obtenção de um substrato de cultivo para células-tronco pluripotentes e substrato de cultivo produzido pelo mesmo Download PDF

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Publication number
WO2012167338A3
WO2012167338A3 PCT/BR2012/000173 BR2012000173W WO2012167338A3 WO 2012167338 A3 WO2012167338 A3 WO 2012167338A3 BR 2012000173 W BR2012000173 W BR 2012000173W WO 2012167338 A3 WO2012167338 A3 WO 2012167338A3
Authority
WO
WIPO (PCT)
Prior art keywords
culture substrate
substrate
stem cells
pluripotent stem
obtaining
Prior art date
Application number
PCT/BR2012/000173
Other languages
English (en)
French (fr)
Other versions
WO2012167338A2 (pt
Inventor
Stevens KASTRUP REHEN
Mariana PARANHOS STELLING
Original Assignee
Universidade Federal Do Rio De Janeiro - Ufrj
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidade Federal Do Rio De Janeiro - Ufrj filed Critical Universidade Federal Do Rio De Janeiro - Ufrj
Publication of WO2012167338A2 publication Critical patent/WO2012167338A2/pt
Publication of WO2012167338A3 publication Critical patent/WO2012167338A3/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

O presente pedido de patente de invenção compreende um substrato para o cultivo de células-tronco pluripotentes, o qual é gerado a partir da fixação por etanol de células de fibroblastos fetais de camundongo, e o processo de obtenção do mesmo. A invenção tem o objetivo de estabelecer um substrato com a complexidade necessária para garantir pelo menos 95% das células mantidas pluripotentes. Além disso, o uso de camadas de células alimentadoras é substituído, as condições de cultivo são mantidas e a contaminação com moléculas animais pela matriz é eliminada, sendo um substrato de baixo custo e compatível com a demanda científica nacional.
PCT/BR2012/000173 2011-06-06 2012-06-06 Processo para a obtenção de um substrato de cultivo para células-tronco pluripotentes e substrato de cultivo produzido pelo mesmo WO2012167338A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI1103059-3 2011-06-06
BRPI1103059-3A BRPI1103059B1 (pt) 2011-06-06 2011-06-06 Processo para a obtenção de um substrato de cultivo para células-tronco pluripotentes e substrato de cultivo produzido pelo mesmo

Publications (2)

Publication Number Publication Date
WO2012167338A2 WO2012167338A2 (pt) 2012-12-13
WO2012167338A3 true WO2012167338A3 (pt) 2013-02-21

Family

ID=47296532

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2012/000173 WO2012167338A2 (pt) 2011-06-06 2012-06-06 Processo para a obtenção de um substrato de cultivo para células-tronco pluripotentes e substrato de cultivo produzido pelo mesmo

Country Status (2)

Country Link
BR (1) BRPI1103059B1 (pt)
WO (1) WO2012167338A2 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113832095B (zh) * 2021-09-26 2024-03-22 河南城建学院 一种乙醇处理制备饲养层的方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175956A1 (en) * 1997-10-23 2003-09-18 Bodnar Andrea G. Feeder-free culture method for embryonic stem cell
US20050124063A1 (en) * 2003-12-09 2005-06-09 Industrial Technology Research Culture system and method for maintenance and proliferation of undifferentiated human embryonic stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175956A1 (en) * 1997-10-23 2003-09-18 Bodnar Andrea G. Feeder-free culture method for embryonic stem cell
US20050124063A1 (en) * 2003-12-09 2005-06-09 Industrial Technology Research Culture system and method for maintenance and proliferation of undifferentiated human embryonic stem cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ITO, YOSHIHIRO ET AL.: "Chemically fixed nurse cells for culturing murine or primate embryonic stem cells.", J BIOSCI BIOENG., vol. 103, no. 2, February 2007 (2007-02-01), pages 113 - 121 *

Also Published As

Publication number Publication date
WO2012167338A2 (pt) 2012-12-13
BRPI1103059A2 (pt) 2013-11-12
BRPI1103059B1 (pt) 2021-09-21

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