WO2012162820A1 - Maleimide-functionalized gold nanoparticles - Google Patents
Maleimide-functionalized gold nanoparticles Download PDFInfo
- Publication number
- WO2012162820A1 WO2012162820A1 PCT/CA2012/000609 CA2012000609W WO2012162820A1 WO 2012162820 A1 WO2012162820 A1 WO 2012162820A1 CA 2012000609 W CA2012000609 W CA 2012000609W WO 2012162820 A1 WO2012162820 A1 WO 2012162820A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- maleimide
- nanoparticle
- formula
- functionalized gold
- gold nanoparticle
- Prior art date
Links
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 101
- 239000010931 gold Substances 0.000 title claims abstract description 84
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 82
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 81
- 239000003446 ligand Substances 0.000 claims description 58
- 238000000034 method Methods 0.000 claims description 35
- 239000003814 drug Substances 0.000 claims description 20
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical compound C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 18
- 238000010438 heat treatment Methods 0.000 claims description 16
- 239000000523 sample Substances 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 229940124597 therapeutic agent Drugs 0.000 claims description 11
- 229940079593 drug Drugs 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 6
- 239000000700 radioactive tracer Substances 0.000 claims description 5
- 239000002356 single layer Substances 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000002243 precursor Substances 0.000 claims description 3
- 238000000701 chemical imaging Methods 0.000 claims description 2
- RZTAMFZIAATZDJ-UHFFFAOYSA-N felodipine Chemical compound CCOC(=O)C1=C(C)NC(C)=C(C(=O)OC)C1C1=CC=CC(Cl)=C1Cl RZTAMFZIAATZDJ-UHFFFAOYSA-N 0.000 claims 1
- 238000003384 imaging method Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 22
- 150000003573 thiols Chemical class 0.000 description 21
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 18
- 239000000243 solution Substances 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 12
- 238000006845 Michael addition reaction Methods 0.000 description 12
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 11
- 229920001223 polyethylene glycol Polymers 0.000 description 11
- 210000004556 brain Anatomy 0.000 description 10
- 239000002202 Polyethylene glycol Substances 0.000 description 9
- 241000700159 Rattus Species 0.000 description 9
- 125000005439 maleimidyl group Chemical group C1(C=CC(N1*)=O)=O 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 238000005481 NMR spectroscopy Methods 0.000 description 6
- 230000008499 blood brain barrier function Effects 0.000 description 6
- 210000001218 blood-brain barrier Anatomy 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 238000009826 distribution Methods 0.000 description 6
- 125000001424 substituent group Chemical group 0.000 description 6
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 5
- 238000006742 Retro-Diels-Alder reaction Methods 0.000 description 5
- 125000000217 alkyl group Chemical group 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000000163 radioactive labelling Methods 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 238000002411 thermogravimetry Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 4
- 238000000026 X-ray photoelectron spectrum Methods 0.000 description 4
- 150000001336 alkenes Chemical group 0.000 description 4
- 210000000988 bone and bone Anatomy 0.000 description 4
- 210000001638 cerebellum Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- RMVRSNDYEFQCLF-UHFFFAOYSA-N phenyl mercaptan Natural products SC1=CC=CC=C1 RMVRSNDYEFQCLF-UHFFFAOYSA-N 0.000 description 4
- 238000012636 positron electron tomography Methods 0.000 description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- TUFFYSFVSYUHPA-UHFFFAOYSA-M rhodamine 123 Chemical compound [Cl-].COC(=O)C1=CC=CC=C1C1=C(C=CC(N)=C2)C2=[O+]C2=C1C=CC(N)=C2 TUFFYSFVSYUHPA-UHFFFAOYSA-M 0.000 description 4
- 229910052717 sulfur Inorganic materials 0.000 description 4
- 239000011593 sulfur Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000005160 1H NMR spectroscopy Methods 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 0 C*(CCO*(CCSC)C(C)(C)*NS*C1(C)*2CCCC1C2)C(C)(CCN)IC Chemical compound C*(CCO*(CCSC)C(C)(C)*NS*C1(C)*2CCCC1C2)C(C)(CCN)IC 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 238000005698 Diels-Alder reaction Methods 0.000 description 3
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000012377 drug delivery Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 3
- -1 isodecyl Chemical group 0.000 description 3
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 229910000073 phosphorus hydride Inorganic materials 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000004611 spectroscopical analysis Methods 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 238000012453 sprague-dawley rat model Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- ZIBGPFATKBEMQZ-UHFFFAOYSA-N triethylene glycol Chemical compound OCCOCCOCCO ZIBGPFATKBEMQZ-UHFFFAOYSA-N 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 238000003917 TEM image Methods 0.000 description 2
- 125000003545 alkoxy group Chemical group 0.000 description 2
- 125000004453 alkoxycarbonyl group Chemical group 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 125000003710 aryl alkyl group Chemical group 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- OBMPJXWHIMIXSH-UHFFFAOYSA-N benzenethiol Chemical compound SC1=CC=CC=C1.SC1=CC=CC=C1 OBMPJXWHIMIXSH-UHFFFAOYSA-N 0.000 description 2
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 238000006352 cycloaddition reaction Methods 0.000 description 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
- 235000018417 cysteine Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010253 intravenous injection Methods 0.000 description 2
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 2
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 125000001971 neopentyl group Chemical group [H]C([*])([H])C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 2
- 230000014207 opsonization Effects 0.000 description 2
- 239000012044 organic layer Substances 0.000 description 2
- 238000006053 organic reaction Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 2
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 2
- 239000011877 solvent mixture Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 125000000547 substituted alkyl group Chemical group 0.000 description 2
- QBVXKDJEZKEASM-UHFFFAOYSA-M tetraoctylammonium bromide Chemical compound [Br-].CCCCCCCC[N+](CCCCCCCC)(CCCCCCCC)CCCCCCCC QBVXKDJEZKEASM-UHFFFAOYSA-M 0.000 description 2
- 238000004627 transmission electron microscopy Methods 0.000 description 2
- AGBQKNBQESQNJD-SSDOTTSWSA-N (R)-lipoic acid Chemical compound OC(=O)CCCC[C@@H]1CCSS1 AGBQKNBQESQNJD-SSDOTTSWSA-N 0.000 description 1
- 125000005917 3-methylpentyl group Chemical group 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000219495 Betulaceae Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000978750 Havardia Species 0.000 description 1
- PIWKPBJCKXDKJR-UHFFFAOYSA-N Isoflurane Chemical compound FC(F)OC(Cl)C(F)(F)F PIWKPBJCKXDKJR-UHFFFAOYSA-N 0.000 description 1
- 241000306729 Ligur Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 239000012901 Milli-Q water Substances 0.000 description 1
- 238000012879 PET imaging Methods 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- YZCKVEUIGOORGS-IGMARMGPSA-N Protium Chemical compound [1H] YZCKVEUIGOORGS-IGMARMGPSA-N 0.000 description 1
- 241000287181 Sturnus vulgaris Species 0.000 description 1
- YTGJWQPHMWSCST-UHFFFAOYSA-N Tiopronin Chemical compound CC(S)C(=O)NCC(O)=O YTGJWQPHMWSCST-UHFFFAOYSA-N 0.000 description 1
- 108010058907 Tiopronin Proteins 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- AGBQKNBQESQNJD-UHFFFAOYSA-N alpha-Lipoic acid Natural products OC(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000001949 anaesthesia Methods 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000004037 angiogenesis inhibitor Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000001028 anti-proliverative effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 229940121357 antivirals Drugs 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 150000003842 bromide salts Chemical group 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 150000005829 chemical entities Chemical class 0.000 description 1
- 239000007810 chemical reaction solvent Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 229940126214 compound 3 Drugs 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- NZNMSOFKMUBTKW-UHFFFAOYSA-N cyclohexanecarboxylic acid Chemical compound OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 1
- 125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 150000001993 dienes Chemical class 0.000 description 1
- IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 150000002019 disulfides Chemical class 0.000 description 1
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 239000006167 equilibration buffer Substances 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 229940116589 hepatic and reticulo endothelial system diagnostic radiopharmaceuticals Drugs 0.000 description 1
- 125000003187 heptyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 229960002725 isoflurane Drugs 0.000 description 1
- 125000004491 isohexyl group Chemical group C(CCC(C)C)* 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000019136 lipoic acid Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- LLAZQXZGAVBLRX-UHFFFAOYSA-N methyl 2,5-dioxopyrrole-1-carboxylate Chemical compound COC(=O)N1C(=O)C=CC1=O LLAZQXZGAVBLRX-UHFFFAOYSA-N 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 125000001400 nonyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000005935 nucleophilic addition reaction Methods 0.000 description 1
- 238000010534 nucleophilic substitution reaction Methods 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003003 phosphines Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000425 proton nuclear magnetic resonance spectrum Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000005258 radioactive decay Effects 0.000 description 1
- 230000003439 radiotherapeutic effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- VUFNRPJNRFOTGK-UHFFFAOYSA-M sodium;1-[4-[(2,5-dioxopyrrol-1-yl)methyl]cyclohexanecarbonyl]oxy-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].O=C1C(S(=O)(=O)[O-])CC(=O)N1OC(=O)C1CCC(CN2C(C=CC2=O)=O)CC1 VUFNRPJNRFOTGK-UHFFFAOYSA-M 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- DZLFLBLQUQXARW-UHFFFAOYSA-N tetrabutylammonium Chemical compound CCCC[N+](CCCC)(CCCC)CCCC DZLFLBLQUQXARW-UHFFFAOYSA-N 0.000 description 1
- 229940071240 tetrachloroaurate Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- DUYAAUVXQSMXQP-UHFFFAOYSA-M thioacetate Chemical compound CC([S-])=O DUYAAUVXQSMXQP-UHFFFAOYSA-M 0.000 description 1
- 229960002663 thioctic acid Drugs 0.000 description 1
- 150000007944 thiolates Chemical class 0.000 description 1
- 229960004402 tiopronin Drugs 0.000 description 1
- CMQCNTNASCDNGR-UHFFFAOYSA-N toluene;hydrate Chemical compound O.CC1=CC=CC=C1 CMQCNTNASCDNGR-UHFFFAOYSA-N 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 125000002948 undecyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
- A61K49/0041—Xanthene dyes, used in vivo, e.g. administered to a mice, e.g. rhodamines, rose Bengal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
- A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
- A61K51/04—Organic compounds
- A61K51/06—Macromolecular compounds, carriers being organic macromolecular compounds, i.e. organic oligomeric, polymeric, dendrimeric molecules
- A61K51/065—Macromolecular compounds, carriers being organic macromolecular compounds, i.e. organic oligomeric, polymeric, dendrimeric molecules conjugates with carriers being macromolecules
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
- A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
- A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
- A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
- A61K49/0054—Macromolecular compounds, i.e. oligomers, polymers, dendrimers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0063—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres
- A61K49/0065—Preparation for luminescence or biological staining characterised by a special physical or galenical form, e.g. emulsions, microspheres the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
- A61K51/12—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules
- A61K51/1241—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules particles, powders, lyophilizates, adsorbates, e.g. polymers or resins for adsorption or ion-exchange resins
- A61K51/1244—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules particles, powders, lyophilizates, adsorbates, e.g. polymers or resins for adsorption or ion-exchange resins microparticles or nanoparticles, e.g. polymeric nanoparticles
- A61K51/1251—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules particles, powders, lyophilizates, adsorbates, e.g. polymers or resins for adsorption or ion-exchange resins microparticles or nanoparticles, e.g. polymeric nanoparticles micro- or nanospheres, micro- or nanobeads, micro- or nanocapsules
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/12—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains three hetero rings
- C07D491/18—Bridged systems
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/333—Polymers modified by chemical after-treatment with organic compounds containing nitrogen
- C08G65/33331—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing imide group
- C08G65/33334—Polymers modified by chemical after-treatment with organic compounds containing nitrogen containing imide group acyclic
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/334—Polymers modified by chemical after-treatment with organic compounds containing sulfur
- C08G65/3344—Polymers modified by chemical after-treatment with organic compounds containing sulfur containing oxygen in addition to sulfur
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/70—Nanostructure
- Y10S977/773—Nanoparticle, i.e. structure having three dimensions of 100 nm or less
- Y10S977/775—Nanosized powder or flake, e.g. nanosized catalyst
- Y10S977/777—Metallic powder or flake
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
- Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
Definitions
- the present specification broadly relates to maleimide- functionalized gold nanoparticles. More specifically, but not exclusively the present specification relates to maleimide-functionalized gold nanoparticles for multimodal biological applications. The present specification also relates to a process for the preparation of maleimide-functionalized gold nanoparticles.
- Ligand-capped gold nanoparticles which consist of a small gold core and a monolayer of ligands have been extensively studied and characterized in the last two decades. 11,21 Their high chemical stability, low toxicity, and the ease by which their surface can be designed, makes gold nanoparticles ideal bio-probes or vectors for probe/drug delivery in the application of biological diagnostics and therapeutics. (3 5) Water soluble gold nanoparticles synthesized by the Turkevich method provide access to lelatively large AuNP (>10 nm diameter).' 61 [0004] Most AuNP studies relate to alkylthiolated protected gold nanoparticles, which usually have excellent stability in organic solvents and can be manipulated like many small organic molecules.
- citrate serves both as the reducing reagent (to reduce HAuCI 4 in water) and as the capping ligand preventing the gold cores from aggregating.
- the size distribution of the citrate-capped AuNP can be controlled to vary between 5 to 147 nm by a selective choosing of the temperature, the ratio of gold to citrate, and the sequence of adding reagents.' 7 51
- weak ionic interactions limit the stability of these AuNPs
- the NPs prepared using this method cannot easily be separated from water and re-dispersed in water after storage.
- PEG Polyethylene glycol
- Mono-maleimide functionalized gold nanoparticles huvt been previously prepared by Hainfeld and coworkers 115 161
- the maleimide containing monolayer protected nanoparticle (MPN) is prepared by mixing fluorescein- conjugated phosphine ligand protected MPNs with a 100 fold excess of N- methoxycarbonylmaleimide
- This phosphine protected nanoparticle however suffers from low stability. It has to be stored at -4 U C and must be used at low temperatures.
- the low thermal stability reduces its potential for incubation conditions at 37"C More importantly, it can degrade upon exposure to thiols, as thiols can undergo not only the desired Michael addition reaction but also the fast place-exchange reactions, replacing the weaKly bound phosphine ligands.
- Zhu et el. have prepared maleimide-MPNs which can dissolve in toluene, benzene, dichloromethane, and other organic solvents.' 171 They also studied cycloaddition reactions involving the maleimide-MPNs with dienes or nitrones to further modify the nanoparticles under harsh conditions, e g. 96,000 atm.
- the present specification broadly relates to maleimide- functionalized gold nanoparticles
- the present specification relates to a maleimide-functionalized gold nanoparticle of Formula I:
- n and m are integers independently ranging from 1 to 100.
- the present specification relates to a maleimide-functionalized gold nanoparticle of Formula II: Formula II wherein "n” and “m” are integers independently ranging from 1 [0016] In a further embodiment, the present specification relates to a maleimide-functionaiized gold nanoparticle-based bioprobe of Formula HI
- n and m are integers independently ranging from 1 to 100;
- X is selected from the group consisting of NR and S;
- R 1 is H or C 1-H alkyl
- B is a probe or a therapeutic agent (for example a biomolecular probe), and
- z is an integer ranging from 0 to 5.
- the present specification relates lo a radiolabeled maleimide-functionaiized gold nanoparticle of Formula IV;
- n and m are integers independently ranging from 1 to 100.
- the present specification relates to a furan protected maleimide-PEG-thiol having the formula:
- n is an integer ranging from 1 to 100.
- the present specification relates to a maleimide-functionalized gold nanoparticle comprising from about 950 to about 2300 gold atoms.
- the present specification relalos to a maleimide-functionalized gold nanoparticle comprising about 1000 gold atoms.
- the present specification relates to a maleimide-functionalized gold nanoparticle comprising a gold core having an average size ranging from 3 to 4 nm.
- the present specification relates to a maleimide-functionalized gold nanoparticle comprising a gold core having an average size of about 3.2 nm.
- the present specification relates to a maleimide-functionalized gold nanoparticle comprising maleimide-terminated ligands and PEG ligands.
- the maleimide-terminated ligands are maleimide-terminated PEG ligands
- the present specification relates to a maleimide-functionalized gold nanoparticle comprising maleirnide-terminated ligands and PEG ligands, wherein the ratio of maleimide-terminated ligands to PEG ligands ranges from 1 :1 to 1 :3
- the present specification relates to a water soluble maleimide-functionalized gold nanoparticle.
- the present specification relates to a process for preparing a maleimide-functionalized gold nanoparticle, the process comprising reacting PEGylated AuNPs with a furan-protected maleimide-PEG- thiol followed by the removal of the furan-protection group.
- the present specification relates to a process for preparing a maleimide- functionalized gold nanoparticle of Formula I comprising heating a maleimide- functionalized gold nanoparticle of Formula II under conditions lu fof rn the maleimide-functionalized gold nanoparticle of Formula I.
- the reactive maleimide group is generated by heating the functionalized gold nanoparticle of Formula II in a suitable solvent or solvent mixture at a temperature ranging from about 50 to 150°C.
- the present specification relates to the use of a maleimide-functionalized gold nanoparticle for multimodal biological applications.
- the present specification relates to the use of a radiolabeled maleimide-functionalized gold nanoparticle of Formula IV as a radiotracer in spectroscopic imaging applications.
- the present specification relates to a method for delivering a therapeutic agent to a subject, the method comprising:
- the present specification relates to a method for delivering a radioimmaging agent to a subject, the method comprising:
- the present specification relates to a method of using a radiolabeled maleimide-functionalized gold nanoparlicle of Formula IV as a radiotracer for radioimaging applications, the method comprising administering the radiolabeled maleimide-functionalized gold nanoparticle of Formula IV to a subject.
- FIG. 1 is an illustration of the monitoring of the retro-Diels-
- FIG. 2 is a Transmission Electron Microscopy (TEM) image of
- 6-AuNP and Maleimide-AuNP scale bar is 20 nm.
- FIG. 3 is a Thermal Gravimetric Analysis (TGA) of 6-AulMP and maleimide-AuNP (programmed temperature rising speed l0°C/rnin.).
- FIG. 4 is an illustration of the X-ray Photoelectron bpectiurn
- FIG. 5 is an illustration of the fluorescent spectra for
- Rhodamine-MPN before and after treatment with l 2 /KI (excited at 505 nm and emission at 530 nm).
- FIG. 6 is an illustration of the 1 H NMR spectrum of a maleimide-AuNP; peaks at 5.82 ppm and 2.80 ppm correspond to the alkene protons of the maleimide and the protons a to the sulfur, respectively (solvent D 2 O).
- each maleimide-PEG ligand contains 2 alkene protons in addition to 2 protons a to sulfur and each PEG ligand contains 2 protons a to sulfur
- the mole ratio of the maleimide-PEG-thiolated ligand can be determined from the integration as being 1 :2.
- FIG. 7 is an illustration of the radiolabeling of a maleimide-
- the radiolabeling comprises: 1 ) the 18 F labeling of SiFA-SH to produce [ ie F]$iFA-SH; 2) reacting [ ,B F]SiFA-SH with a maleimide-AuNP; and 3) purification of the [ ie F]SiFA-AuP by SEC.
- FIG. 8 is an illustration of representative body and brain scan images of a micro PET scan following intravenous injection of a solution of
- 18 F]- SiFA-AuNP in rats. Broadly, the images represent coronal (a), sagittal (b) and transverse planes (c); and brain scan images in coronal (d), sagittal (e) and transverse planes (f). Sum images t 60-120 min.
- FIG. 10 is an illustration of the biodistribution of [ 1M F]-SiFA-
- AuNP in selected tissues injected dose for each gram of tissue (%ID/g) calculated from the ⁇ vivo biodistribution (left) and zoom of the left figure to Show the distribution of [ l0 F]-SiFA-AuNP (right).
- - SiFA-AuNP was highest in the kidneys (2%), liver ( .5%) and spleen (1.5%)
- the second component as used herein is chemically different from the other components or first component, A “third” component is different from the other, first, and second components, and further enumerated or “additional” components are similarly different
- alkyl can be straight-chain or branched. This also applies if they carry substituents or occur as substituents on other residues, for example in alkoxy residues, alkoxycarbonyl residues or arylalkyl residues. Substituted alkyl residues can be substituted in any suitable position.
- alkyl residues containing from 1 to 18 carbon atoms are methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, dodecyl, tetradecyl, hexadecyl and octadecyl, the f ⁇ -isorners of all these residues, isopropyl, isobutyl, isopentyl, neopentyl, isohexyl, isodecyl, 3- methylpentyl, 2,3,4-trimethylhexyl, sec-butyl, fert-butyl, or fert-pentyl.
- a specific group of alkyl residues is formed by the residues methyl, ethyl, n-propyl, isopropyl, ⁇ -butyl, isobutyl, sec-butyl and rerf-butyl.
- lower alkyl can be straight-chain or branched. This also applies if they carry substituents or occur as substituents on other residues, for example in alkoxy residues, alkoxycarbonyl residues or arylalkyl residues Substituted alkyl residues can be substituted in any suitable position. Examples of lower alkyl residues containing from 1 to 6 carbon aloms are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, isopentyl, neopentyl, and hexyl,
- probe means a chemical entity that probes, examines or tests. The entity will possess a site that interacts with a desired species, for example in solution and for example in a selective and detectable manner
- biomolecular probe refers to a probe that will interact with a biomolecule, such as a protein, peptide, amino acid, nucleic acid, polysaccharide, lipid, phospholipid and the like, for example, in solution and for example in a selective manner.
- therapeutic agent refers to any agent or drug that has a pharmacological effect on a cell or a subject.
- therapeutic agents include anti-inriarnmatory agents, antibiotics, antivirals, antineoplastic/antiangiogenic agents and antiproliferative agents.
- interact means for one entity to associate with another entity in a manner such that the interaction is detectable.
- substituted means that a hydrogen radical of the designated moiety is replaced with the radical of a specified substituent, provided that the substitution results in a stable or chemically feasible compound.
- substituents include halogen (F, CI, Br, or I) for example F, and C h alky!
- a gold nanoparticle can be depicted using either and (AU) Both are used interchangeably throughout tho present specification,
- multimodal means to have more than one mode of operation or function, for example, for the delivery of more lhan one therapeutic agent and/or for probing more than one biomolecule and/or for the delivery of more than one radioimmaging agent.
- suitable means that the selection of the particular compound or conditions would depend on the specific synthetic manipulation to be performed, and the identity of the molcculG(s) to be transformed, but the selection would be well within the skill of a person liained in the art. All process/method steps described herein are to be conducted under conditions sufficient to provide the product shown.
- reaction conditions including, for example, reaction solvent, reaction time, reaction temperature, reaction pressure, reactant ratio and whether or not the reaction should be performed under an anhydrous or inert atmosphere, can be varied to optimize the yield of the desired product and it is within their skill to do so.
- the expression "proceed to a sufficient extent" as used herein with reference to the reactions or process steps disclosed herein means that the reactions or process steps proceed to an extent that conversion of tho starting material or substrate to product is maximized. Conversion may be maximized when greater than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 99% of the starling material or substrate is converted to product.
- subject means a mammal.
- the subject is a human.
- AuNPs The chemical properties of AuNPs are principally determined by the surrounding ligands.
- a variety of functional alkyl-thiolated protected AuNPs can be prepared via direct synthesis, place exchange reactions or post- synthesis reactions. The first two methods require tedious and time-consuming work to prepare each individual thiol. In addition, these methods tend to suffer from low thiol efficiency, especially for the place-exchange reactions which require a large excess of thiol lo rnaleimide ratios to effectively replace the existing ligands.
- ratios of PEG- thiol/furan-protected maleimide-PEG-thiol in excess of 1 :1 for examplo 2 1 , 3 1 , 4:1 , 5:1 , 6:1 , 7:1 , 8:1 , 9:1 10:1 , 20:1 or higher were used to effectively replace the existing ligands.
- a functional AuNP which could be treated as analogous to common organic molecules is an important aspect for its application in a functional platform.
- This template AuNP should contain one or more active end-groups which can be further transformed via commonly used organic reactions, such as nucleophilic substitution reactions, nucleophilic addition reactions, Fisher esterification reactions and/of cycloaddition reactions, as a means to introduce new functional groups.
- a template for use lo post-modify AuNPs optimally has the following characteristics: it should effectively undergo organic reactions under mild conditions; the functional groups on the AuNP should react selectively with the incoming molecules; and the introduced molecules should require only little or no pre-modificalion steps before the installation.
- the maleimide-tln ' ol reaction is a popular reaction used by biochemists to effectively couple a peptide of interest to a substrate Moreover, as a variety of biomolecules are naturally bearing thiol or amino groups, a rnaleimide functionalized nanoparticle is a promising template for use as a carrier for drug delivery or therapeutic diagnostics [0078]
- the present specification relates to an economic, efficient and reproducible method for preparing stable, water-soluble, maleimide-terminated thiolate protected gold nanoparticles
- the present specification relates to the preparation of size controlled PEGylated gold nanoparticles with an average size ranging between about 2-10 nm, following a modified House method.' 201 Subsequently, these PEGylated AuNPs were modified with a furan-protected maleimide-PEG-thiol.
- the resulting furan-protected maleimide-monolayer protected nanoparticle (MPN) has excellent thermal and temporal stability and the reactive maieimide group can be generated via a retro-Diels-Alder reaction simply by heating at 100°C over a period of two hours.
- ⁇ -NMR spectroscopy represents a powerful tool to examine the structure and composition of the MPN monolayers.
- the resonance peaks of the MPN monolayers are characteristically broad mainly due to spin spin relaxation (T 2 ) broadening.
- T 2 spin spin relaxation
- the dense packing of the Iigand chains close to the gold core results in a solid-like character due to dipolar interactions which translate into a fast spin relaxation.
- the short relaxation time results in a broadened and less intense signal.
- the maleimide-NPs prepared in accordance wilh an embodiment of the present specification possess excellent stability and can be purified by washing and drying using regular organic work-up methods. 'H-NMR spectroscopy was applied to monitor the retro- Diels-Alder reaction (for liberation of the maleimide) and was used to verify the purity of MPNs (FIG. 1 ).
- maleimide-NP can effectively react with amine or thiol model compounds via a Michael addition reaction which is indicative that the maleimide-NPs can be used as a platform to prepare; mono- modular or multi-modular probes by applying different combinations of thiol/amino containing probe molecules.
- these maleimide-NPs can also be used as drug delivery vehicles by linking a drug to the maleimide group.
- the drug comprises a thiol and/or amino function that ca be used for linking the drug to the maleimide NPs.
- this druy- linking is accomplished using a thiol/amino containing linker.
- these maleimide-NPs can also be used as radioimmaging delivery vehicles by linking a radioimmaging agent or its precursor to the maleimide group.
- the maleimide-functionalized gold NP could not be prepared by direct synthesis as a strong reducing reagent is usually applied during the reduction of Au(l) to Au(0).
- the desired maleimide-functionalized gold NP could also not be directly prepared via the commonly used place-exchange reaction with maleimide-PEG-thiol, because maleimide is a good thiol-scavenger and the maleimide-tethered thiol is likely to undergo a Michael addition reaction with itself.
- a furan protected maleimide-PEG-thiol (6) was synthesized so that it could be place-exchanged onlo a PEGylated AuNP. The reactive maleimide group could then be generated by heating.
- Schemes 1 and 2 depict the modified House method used for the preparation of the PEGylated nanoparticle' 701 (Scheme 1 ) and the preparation of the maleimide- functionalized gold NP (Scheme 2), respectively
- AuNPs were prepared as follows. Hydrogen tetrachloroaurate (HAuC ) or potassium tetrabromoaurate ( AuBr ) was dissolved in Milli-Q water and then mixed with tetraoctylammonium bromide (TOAB) in toluene. The contents were vigorously stirred in order to facilitate the phase transfer of the Au(lll) into the toluene layer. The organic layer was separated and dried with MgSd in order to remove any residual water and then cooled to 0 'J C in an ice bath.
- HUAC Hydrogen tetrachloroaurate
- AuBr potassium tetrabromoaurate
- TOAB tetraoctylammonium bromide
- Triethylene glycol-thiol "(TEG)-thiol"
- TEG Triethylene glycol-thiol
- a pegylated thiol was added to the solution via a volumetric pipette and allowed to stir for about 10 minutes.
- the observed darK orange solution faded with time.
- the thiol/gold molar ratio exceeded 2:1 , the solution became clear and colorless.
- Different sizes of AuNP can be obtained by varying the gold/thiol ratio.
- a fresh solution of tetrabutylammonium borohydride was then quickly added (in about 5 seconds) to a rapidly stirring toluene solution. The solution instantly turned black.
- the TEGylated NPs began to precipitate From the toluene after about 2 hours.
- TEGylated AuNP After stirring the mixture overnight ("12 hours), 30 ml. of illi-Q water was added to extract the TEGylated AuNP.
- the TEGylated AuNPs were purified by washing with toluene/acetonitrile or dialysis. The resulting pure AuNP sample was assessed by 'H NMR spectroscopy, and showed no sign of free ligand.
- this TEGylated MPN has a smaller size, e.g. 3 nm, good stability, and could be easily prepared, purified, and characterized. It could also be repeatedly dried and readily redissolved in water.
- the key resonances include the broad resonance at 6.35 ppm due to the alkene protons and those at 2.50 ppm due to the fused protons from the Diels-Alder adduct (FIG. 1a).
- the resonance of the methylene bridgehead protons was merged with the solvent peak while at room temperature. However, at an elevated temperature of 90 U C, the solvent peak shifts downfield and the signal at 4.95 ppm can be assigned to the methylene bridgehead protons (FIG. 1 )
- FIG. 1 illustrates the monitoring of the retro-Diels-Alder reaction by varied-temperature 1 H-NMR spectroscopy.
- the broad peak at 5.82 ppm corresponds to the alkene protons of the maleimide wherwas the peaks at 7.45 ppm and 6 50 ppm are attributed to the free furan liberated from the retro-Diels-Alder reaction (the furan peaks eventually disappear after heating for 90 min).
- the maleimide end-group started to be produced while heating and was completely recovered following heating at 90 C over a period of one hour.
- the product ma!eimide-AuNPs were readily soluble in water following drying and could be re-dissolved for several cycles.
- the molar ra!io of maleimide PEG- thiolated ligand to PEGylated ligand was determined from the integration of the ⁇ -NMR spectrum of maleimide-AuNP to be 1 :2 (FIG. 6).
- TEM images of 6- AuNP before and after heating (maleimide-AuNP) showed a small increase of the core size and wider dispersion from 3.0 ⁇ 0.5 nm to 3 2 ⁇ 0 8 run (FIG. 2). This slight increase in core size was due to small quantities of ligand which were liberated as disulfide during heating.
- TGA Thermal gravimetric analysis
- the maleimide-AuNP has a spherical shape.
- the average diameter of the mateimide-AuNP core could be derived from the planar sphere projection (from TEM derived to be 3.2 nm). It was also assumed that the gold core has the same density as bulk gold, the average number of gold atoms per AuNP is thus ca. 1000, using the formula : l2 ]
- N TM ⁇ N A
- N number of atoms per nanoparticle
- d average diameter of the nanoparticles
- N A Avogadro constant
- the total number of ligands per gold nanoparticle can be calculated from the weight percentage of the organic portion of the PN
- the mixed ligand rnaleimide-AuNP is capped with two different ligands (e.g. maleimide-TEG-thiol ligands and TEGylated thiol ligands), and the average protecting ligands number is determined to be 90, using:
- N L total number of ligands per nanopartide
- ⁇ percentage of mass loss due to the protecting ligands
- M L i molecular weight of the maleimide-TEG-thiol ligands
- Mi ? molecular weight of the TEGylated thiol ligands
- ⁇ the molar percentage of the maleimide ligand.
- the area per grafted ligand is thus 0.35 nm 2 , recognizing that this composition is based on using a gold core having a size of 3 2 nm
- the total mass of organic/gold particle was determined, providing for the determination of the relative contribution of the two organic species (ligands) to this total mass. This yielded a weighted average number of molecules per particle. Since the average surface area of the gold core is known, the area occupied per grafted ligand could be determined. An area of 0.35 nm"' is consistent with a moderately bulky thiol ligand.
- the simplified formula of IN: maleimide-AuNP can then be represented as Aui 0 on(TEG)Go( aleirnide)-io.
- X-ray photoelectron spectra (XPS) of the maieimide AuNP confirm the presence of the maieimide group (FIG. 4).
- the nitrogen N 1 s BE value of 400.28 eV indicates the presence of the maieimide on the AuNP surface.
- the sulfur/nitrogen (S:N) ratio was 7:3, which was slightly lower than the calculated ratio of 9:3 (from the ⁇ -NMR integration). This discrepancy likely arises from an attenuation of the S signal relative to the N signal in the XPS experiment, given that the S is directly bonded to the gold surface and is thus buried relative to the N.
- the reactive maieimide end groups of the maleimidc-AuNPs were shown to readily react with amines or thiols via the Michael addition reaction (Scheme 3).
- Cysteine modified biomolecules such as for exam l cystein-derivatii ed oclreotate, represent a large class of bioprobe/precursors.
- the model Michael addition reaction between a maleimide-AuNP and cysteine was studied by ⁇ -l-NM spectroscopy. Although the new product peaks overlap with the resonance signal of the PEGylated ligands, the disappearance of the maleimide alkene proton peaks indicates that the Michael addition reaction proceeds under these conditions.
- Rhodamine 123 was mixed with maleimide-AuNP in an aqueous solution over a period of 1 h.
- the resulting rhodamine-AuNPs were purified by washing with an ethyl acetate:ethano! solvent mixture to remove any free rhodamine 123 until no fluorescence could be detected in the organic layer Because the fluorescence of this fluorophore-coupled NP was quenched by the gold core (FIG. 5)
- the %lD/g value for brain and cerebellum is about 0.13%, about 50% higher than for bone uptake (0.08%), making the AuNP a promising deliveiy platform for the development of new PET tracers or radio-therapeutic agents.
- the [ 1B FJ- SiFA-AuNP was homogenously distributed in the brain and no aggregation could be observed.
- the use of an ,8 F-labeled AuNP for PET studies using a large NP (e.g. 13 nm) was recently reported. 1281 However, the 1S F-Iabeled AuNP could not be purified making an exact determination of its composition very difficult. Furthermore, larger AuNPs were reported as being unable to effectively cross the BBB.
- kidneys had the highest concentration of [ i a F]-SiF ' A-AuNP
- [ 18 F]-SiFA-SH was shown to effectively react with the water soluble maleimide-AuNPs of the present disclosure to generate radioactively labeled [ 8 F]-SiFA-AuNPs.
- these water soluble maleimide- AuNPs comprise a gold core having a size ranging from 3 to 4 nm.
- the labeled SiFA-AuNPs were readily purified by size exclusion column chromatography The radiolabeling was accomplished via a Michael addition reaction and no physical adsorption was observed when using [ 10 F]-SiFA-OH which does not undergo Michael additions.
- the [ 18 F]-SiFA-AuNPs were able to cross the blood brain barrier (BBB) as illustrated by small animal micro-PET studies as w l as by ex vivo biodistribution data.
- BBB blood brain barrier
- Radiosynthesls of [ 18 F]-SiFA-SH: [ i e nfluoride/H a [ , B 0]0 (30 mCi) was passed through a QMA cartridge preconditioned with 10 mL of 0.5M K 2 C0 3 and 10 mL of H a O. The trapped 18 F was then eluted with 1 mL of a stock solution containing 75 mg (0.20 mmol) K.ryptofix2.2.2 and 0.10 rnrnol potassium oxalate in 10 mL of 96/4 acetonitriie/H 2 0 solution.
- Biod is tri button of [ 18 F]-AuNP in Rats by small animal PET;
- the SD rats were euthanized 2h post-injection of the radiotracers.
- the organs were removed, rinsed with physiological saline, blotted dry and placed in pre-weighted tubes.
- the radioactivity in each tube was counted in a gamma counter (Cobra II, Packard Instruments) and used to calculate the percentage of the injected dose per gram of tissue (% ID/g) for each organ.
- the means from three rats are reported.
- Niidome, T Yamagata, M.; Okamoto, Y.; Akiyama, Y.; Takhashi, H., Kawano, T.; Katayama, Y.; and Niidome, Y. J Controlled Release, 2006, 114, 343-347.
Abstract
A maleimide-functionalized gold nanoparticle is described herein. More specifically, a maleimide-functionalized gold nanoparticle of formula (I): is described, wherein "n" and "m" are integers independently ranging from 1 to 100.
Description
TITLE
MALEI IDE-FUNCTIONALIZED GOLD NANOPARTICLES
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application claims the benefit of priority from copending U.S. provisional application no. 61/491 ,638 filed on May 31 , 2011 , the contents of which are incorporated herein by reference in their entirety.
FIELD
[0002] The present specification broadly relates to maleimide- functionalized gold nanoparticles. More specifically, but not exclusively the present specification relates to maleimide-functionalized gold nanoparticles for multimodal biological applications. The present specification also relates to a process for the preparation of maleimide-functionalized gold nanoparticles.
BACKGROUND
[0003] Ligand-capped gold nanoparticles (L-AuNP) which consist of a small gold core and a monolayer of ligands have been extensively studied and characterized in the last two decades.11,21 Their high chemical stability, low toxicity, and the ease by which their surface can be designed, makes gold nanoparticles ideal bio-probes or vectors for probe/drug delivery in the application of biological diagnostics and therapeutics. (3 5) Water soluble gold nanoparticles synthesized by the Turkevich method provide access to lelatively large AuNP (>10 nm diameter).'61
[0004] Most AuNP studies relate to alkylthiolated protected gold nanoparticles, which usually have excellent stability in organic solvents and can be manipulated like many small organic molecules. In the Turkevich method, citrate serves both as the reducing reagent (to reduce HAuCI4 in water) and as the capping ligand preventing the gold cores from aggregating. The size distribution of the citrate-capped AuNP can be controlled to vary between 5 to 147 nm by a selective choosing of the temperature, the ratio of gold to citrate, and the sequence of adding reagents.'7 51 However, weak ionic interactions limit the stability of these AuNPs, Moreover, the NPs prepared using this method cannot easily be separated from water and re-dispersed in water after storage.
[0005] The use of tiopronin (N-(2*mercaptopropionyl)-glycine) as the protecting ligand to synthesize stable, thiolate-protected water-soluble AuNPs has been previously described. [y} Unfortunately, the charge of the tiopronin- AuNPs prohibits their in-vivo application, as charged particles are susceptible to opsonization by proteins.111,1
[0006] Polyethylene glycol (PEG) has been used to coat gold nanoparticles to reduce toxicity in wVo.111,121 The PEGylated species can reduce the interactions between proteins and nanoparticle surfaces through hydrophilicity and steric repulsion, thus reducing opsonization. It has been previously shown that PEGylated gold nanorods exhibit enhanced circulalion time and demonstrate no cytotoxicity in vitro.[V4] Similarly, studies on the in vitro permeation of gold nanoparticles through rat skin and intestine, show that the larger the gold nanoparticles, the weaker their permeation ability.1'41 However, these studies were dealing with larger nanoparticles (>15 nm).
[0007] Mono-maleimide functionalized gold nanoparticles huvt: been previously prepared by Hainfeld and coworkers 115 161 The maleimide containing
monolayer protected nanoparticle (MPN) is prepared by mixing fluorescein- conjugated phosphine ligand protected MPNs with a 100 fold excess of N- methoxycarbonylmaleimide This phosphine protected nanoparticle however suffers from low stability. It has to be stored at -4UC and must be used at low temperatures. The low thermal stability reduces its potential for incubation conditions at 37"C More importantly, it can degrade upon exposure to thiols, as thiols can undergo not only the desired Michael addition reaction but also the fast place-exchange reactions, replacing the weaKly bound phosphine ligands.
[0008] Zhu et el. have prepared maleimide-MPNs which can dissolve in toluene, benzene, dichloromethane, and other organic solvents.'171 They also studied cycloaddition reactions involving the maleimide-MPNs with dienes or nitrones to further modify the nanoparticles under harsh conditions, e g. 96,000 atm.
[0009] Recently, Rodriguez et al synthesized maleimide-MPNs by ligand place-exchange of CTAB (cetyltrimethylammonium bromide) with amine- terminated PEG ligands, followed by subsequent treatment with a maleimide bearing bio-linker, such as sulfo-SMCC (sulfosuccinirnidyl-4-[N- maleimidomethyl]cyclohexane-1-carboxylate) to prepare the desired NPs.l l£1) A similar study was carried out by Oh et al via place-exchange of the citric acid protected NPs with a PEGylated ligand functionaiized with thioctic acid and maleimide groups in both chain ends.t19] However, both of these studies related to large nanoparticles which were limited by the size of the starting NPs
[001 ] The present specification refers to a number of documents, the contents of which are herein incorporated by reference in their entirety.
SUMMARY
[0011] The present specification broadly relates to maleimide- functionalized gold nanoparticles
[0012] In an embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle of Formula I:
[0013] wherein "n" and "m" are integers independently ranging from 1 to 100.
[0014] In anolher embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle of Formula II:
Formula II wherein "n" and "m" are integers independently ranging from 1
[0016] In a further embodiment, the present specification relates to a maleimide-functionaiized gold nanoparticle-based bioprobe of Formula HI
Formula III
[0017] wherein "n" and "m" are integers independently ranging from 1 to 100;
[0018] X is selected from the group consisting of NR and S;
[0019] R1 is H or C1-Halkyl;
[0020] B is a probe or a therapeutic agent (for example a biomolecular probe), and
[0021] "z" is an integer ranging from 0 to 5.
[0022] In a further embodiment, the present specification relates lo a radiolabeled maleimide-functionaiized gold nanoparticle of Formula IV;
[0023] wherein "n" and "m" are integers independently ranging from 1 to 100.
[0024] In yet a further embodiment, the present specification relates to a furan protected maleimide-PEG-thiol having the formula:
[0025] wherein "n" is an integer ranging from 1 to 100.
[0026] In an embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle comprising from about 950 to about 2300 gold atoms.
[0027] In an embodiment, the present specification relalos to a maleimide-functionalized gold nanoparticle comprising about 1000 gold atoms.
[0028] In an embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle comprising a gold core having an average size ranging from 3 to 4 nm.
[0029] In an embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle comprising a gold core having an average size of about 3.2 nm.
[0030] In an embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle comprising maleimide-terminated ligands and PEG ligands. In an embodiment, the maleimide-terminated ligands are maleimide-terminated PEG ligands
[0031] In an embodiment, the present specification relates to a maleimide-functionalized gold nanoparticle comprising maleirnide-terminated ligands and PEG ligands, wherein the ratio of maleimide-terminated ligands to PEG ligands ranges from 1 :1 to 1 :3
[0032] In an embodiment, the present specification relates to a water soluble maleimide-functionalized gold nanoparticle.
[0033] In an embodiment, the present specification relates to a process for preparing a maleimide-functionalized gold nanoparticle, the process comprising reacting PEGylated AuNPs with a furan-protected maleimide-PEG- thiol followed by the removal of the furan-protection group. In an embodiment, the present specification relates to a process for preparing a maleimide- functionalized gold nanoparticle of Formula I comprising heating a maleimide- functionalized gold nanoparticle of Formula II under conditions lu fof rn the
maleimide-functionalized gold nanoparticle of Formula I. In an embodiment of the prese l specification, the reactive maleimide group is generated by heating the functionalized gold nanoparticle of Formula II in a suitable solvent or solvent mixture at a temperature ranging from about 50 to 150°C.
[0034] In an embodiment, the present specification relates to the use of a maleimide-functionalized gold nanoparticle for multimodal biological applications.
[0035] In an embodiment, the present specification relates to the use of a radiolabeled maleimide-functionalized gold nanoparticle of Formula IV as a radiotracer in spectroscopic imaging applications.
[0036] In an embodiment, the present specification relates to a method for delivering a therapeutic agent to a subject, the method comprising:
[0037] providing a nanoparticle of Formula I;
[0038] coupling the therapeutic agent to the nanoparticle of Formula I to form a nanoparticle-drug conjugate; and
[0039] administering to the subject the nanoparticle-drug conjugate.
[0040] In an embodiment, the present specification relates to a method for delivering a radioimmaging agent to a subject, the method comprising:
[0041] providing a nanoparticle of Formula I;
[0042] coupling the radioimmaging agent to the nanoparlicle of
Formula I to form a nanoparticle- radioimmaging agent conjugate; and
[0043] administering to the subject the nanoparticle-radiotmmaging agent conjugate.
[0044] in an embodiment, the present specification relates to a method of using a radiolabeled maleimide-functionalized gold nanoparlicle of Formula IV as a radiotracer for radioimaging applications, the method comprising administering the radiolabeled maleimide-functionalized gold nanoparticle of Formula IV to a subject.
[0045] The foregoing and other objects, advantages and features of the present specification will become more apparent upon reading of the following non-restrictive description of illustrative embodiments thereof, given by way of example only with reference to the accompanying drawings/ligures.
BRIEF DESCRIPTION OF THE DRAWINGS/FIGURES [0046] In the appended drawings/figures:
[0047] FIG, 1 is an illustration of the monitoring of the retro-Diels-
Alder reaction of 6-AuNP, obtained in accordance with an embodiment of the present specification, by Ή NMR spectroscopy (solvent D2O); 6-AuNP at room temperature (FIG. 1a); 6-AuNP at 90"C (FIG. 1 b), 6-AuNP following heating at 90°C over a period of 20 minutes (FIG, 1c); 6-AuNP following heating at 90°C over a period of 30 minutes (FIG. id); 6-AuNP following heating at 9Q°C over a period of 40 minutes (FIG. 1e); 6-AuNP following heating at 90°C over a period of 50 minutes (FIG. 1f); 6-AuNP following heating at 90°C over a period of 60
minutes (FIG. 1g); downward arrows and upward arrows corresponding to the resonance of the Diels-Alder adducts and the maleimide alkene protons, respectively.
[004B] FIG. 2 is a Transmission Electron Microscopy (TEM) image of
6-AuNP and Maleimide-AuNP (scale bar is 20 nm).
[0049] FIG. 3 is a Thermal Gravimetric Analysis (TGA) of 6-AulMP and maleimide-AuNP (programmed temperature rising speed l0°C/rnin.).
[0050] FIG. 4 is an illustration of the X-ray Photoelectron bpectiurn
(XPS) of maleimide-AuNP, N 1s and S 2p peaks are indicated.
[0051] FIG. 5 is an illustration of the fluorescent spectra for
Rhodamine-MPN before and after treatment with l2/KI (excited at 505 nm and emission at 530 nm).
[0052] FIG. 6 is an illustration of the 1H NMR spectrum of a maleimide-AuNP; peaks at 5.82 ppm and 2.80 ppm correspond to the alkene protons of the maleimide and the protons a to the sulfur, respectively (solvent D2O). As each maleimide-PEG ligand contains 2 alkene protons in addition to 2 protons a to sulfur and each PEG ligand contains 2 protons a to sulfur, the mole ratio of the maleimide-PEG-thiolated ligand. EG-thiolated ligand can be determined from the integration as being 1 :2.
[0053] FIG. 7 is an illustration of the radiolabeling of a maleimide-
AuNP with [18F]-SiFA-SH. The upper portion depicts the radiolabeling reaction whereas the lower portion illustrates the time line for the maleimide-AuNP
labeling. Broadly, the radiolabeling comprises: 1 ) the 18F labeling of SiFA-SH to produce [ieF]$iFA-SH; 2) reacting [,BF]SiFA-SH with a maleimide-AuNP; and 3) purification of the [ieF]SiFA-AuP by SEC.
[0054] FIG. 8 is an illustration of representative body and brain scan images of a micro PET scan following intravenous injection of a solution of | 18F]- SiFA-AuNP in rats. Broadly, the images represent coronal (a), sagittal (b) and transverse planes (c); and brain scan images in coronal (d), sagittal (e) and transverse planes (f). Sum images t = 60-120 min.
[0055] FIG. 9 is an illustration of Ihe time-activity curves for cerebellum, brain and bone extracted from in vivo micro PET scans for up to 60 min post injection of [18F]-SiFA-AuNP. Each data point represents the average of three independent animal experiments. The bars represent errors which are expressed as SEM (n =3).
[0056] FIG. 10 is an illustration of the biodistribution of [1MF]-SiFA-
AuNP in selected tissues; injected dose for each gram of tissue (%ID/g) calculated from the ΘΧ vivo biodistribution (left) and zoom of the left figure to Show the distribution of [l0F]-SiFA-AuNP (right). The concentration of f18F|- SiFA-AuNP was highest in the kidneys (2%), liver ( .5%) and spleen (1.5%)
DETAILED DESCRIPTION
I. Definitions
[0057] In order to provide a clear and consistent understanding of the terms used in the present specification, a number of definitions are provided
below. Moreover, unless defined otherwise, all technical and scientific terms as used herein have the same meaning as commonly understood by one ot ordinary skill in the art to which this specification pertains.
[0058] Unless otherwise indicated, the definitions and embodiments described in this and other sections are intended to be applicable to all embodiments and aspects of the application herein described for which they are suitable as would be understood by a person skilled in the art.
[0059] The use of the word "a" or "an" when used in conjunction with the term "comprising" in the claims and/or the specification may mean "one ", but it is also consistent with the meaning of "one or more", "at least one", and "one or more than one". Similarly, the word "another" may mean at least a second or more.
[0060] In embodiments comprising an "additional" or 'second" component, such as an additional or second nanoparticle, the second component as used herein is chemically different from the other components or first component, A "third" component is different from the other, first, and second components, and further enumerated or "additional" components are similarly different
[0061] As used in this specification and claim(s), the words
"comprising" (and any form of comprising, such as "comprise" and "comprises"), having (and any form of having, such as "have" and "has"), "including" (and any form of including, such as "include" and "includes"), or "containing" (and any form of containing, such as "contain" and "contains"), are inclusive and open- ended and do not exclude additional, unrecited elements or process steps.
[0062] The term "about" is used to indicate that a value includes an inherent variation of error for the device or the method being employed to determine the value.
[0063] As used herein, the term "alkyl" can be straight-chain or branched. This also applies if they carry substituents or occur as substituents on other residues, for example in alkoxy residues, alkoxycarbonyl residues or arylalkyl residues. Substituted alkyl residues can be substituted in any suitable position. Examples of alkyl residues containing from 1 to 18 carbon atoms are methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, dodecyl, tetradecyl, hexadecyl and octadecyl, the f}-isorners of all these residues, isopropyl, isobutyl, isopentyl, neopentyl, isohexyl, isodecyl, 3- methylpentyl, 2,3,4-trimethylhexyl, sec-butyl, fert-butyl, or fert-pentyl. A specific group of alkyl residues is formed by the residues methyl, ethyl, n-propyl, isopropyl, π-butyl, isobutyl, sec-butyl and rerf-butyl.
[0064] As used herein, the term "lower alkyl" can be straight-chain or branched. This also applies if they carry substituents or occur as substituents on other residues, for example in alkoxy residues, alkoxycarbonyl residues or arylalkyl residues Substituted alkyl residues can be substituted in any suitable position. Examples of lower alkyl residues containing from 1 to 6 carbon aloms are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, isopentyl, neopentyl, and hexyl,
[0065] As used herein, the term "probe" means a chemical entity that probes, examines or tests. The entity will possess a site that interacts with a desired species, for example in solution and for example in a selective and detectable manner,
[0066] The term "biomolecular" probe as used herein refers to a probe that will interact with a biomolecule, such as a protein, peptide, amino acid, nucleic acid, polysaccharide, lipid, phospholipid and the like, for example, in solution and for example in a selective manner.
[0067] The term "therapeutic agent" as used herein refers to any agent or drug that has a pharmacological effect on a cell or a subject. Non- limiting examples of therapeutic agents include anti-inriarnmatory agents, antibiotics, antivirals, antineoplastic/antiangiogenic agents and antiproliferative agents.
[0068] The term "interact" as used herein means for one entity to associate with another entity in a manner such that the interaction is detectable.
[0069] The term "substituted" as used herein, means that a hydrogen radical of the designated moiety is replaced with the radical of a specified substituent, provided that the substitution results in a stable or chemically feasible compound. Non-limiting examples of substituents include halogen (F, CI, Br, or I) for example F, and Chalky!
[0070] As used herein, a gold nanoparticle can be depicted using either and (AU) Both are used interchangeably throughout tho present specification,
[0071] As used herein, the expression "multimodal" means to have more than one mode of operation or function, for example, for the delivery of more lhan one therapeutic agent and/or for probing more than one biomolecule and/or for the delivery of more than one radioimmaging agent.
[0072] The term "suitable" as used herein means that the selection of the particular compound or conditions would depend on the specific synthetic manipulation to be performed, and the identity of the molcculG(s) to be transformed, but the selection would be well within the skill of a person liained in the art. All process/method steps described herein are to be conducted under conditions sufficient to provide the product shown. A person skilled in the art would understand that all reaction conditions, including, for example, reaction solvent, reaction time, reaction temperature, reaction pressure, reactant ratio and whether or not the reaction should be performed under an anhydrous or inert atmosphere, can be varied to optimize the yield of the desired product and it is within their skill to do so.
[0073] The expression "proceed to a sufficient extent" as used herein with reference to the reactions or process steps disclosed herein means that the reactions or process steps proceed to an extent that conversion of tho starting material or substrate to product is maximized. Conversion may be maximized when greater than about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 99% of the starling material or substrate is converted to product.
[0074] The term "subject" as used herein means a mammal.
Typically the subject is a human.
II. Gold Nanoparticl s and Compositions and Uses Thereof
[0075] The chemical properties of AuNPs are principally determined by the surrounding ligands. A variety of functional alkyl-thiolated protected AuNPs can be prepared via direct synthesis, place exchange reactions or post-
synthesis reactions. The first two methods require tedious and time-consuming work to prepare each individual thiol. In addition, these methods tend to suffer from low thiol efficiency, especially for the place-exchange reactions which require a large excess of thiol lo rnaleimide ratios to effectively replace the existing ligands. In an embodiment of the present specification, ratios of PEG- thiol/furan-protected maleimide-PEG-thiol in excess of 1 :1 for examplo 2 1 , 3 1 , 4:1 , 5:1 , 6:1 , 7:1 , 8:1 , 9:1 10:1 , 20:1 or higher were used to effectively replace the existing ligands.
[0076) The development of a functional AuNP which could be treated as analogous to common organic molecules is an important aspect for its application in a functional platform. This template AuNP should contain one or more active end-groups which can be further transformed via commonly used organic reactions, such as nucleophilic substitution reactions, nucleophilic addition reactions, Fisher esterification reactions and/of cycloaddition reactions, as a means to introduce new functional groups. Moreover, a template for use lo post-modify AuNPs optimally has the following characteristics: it should effectively undergo organic reactions under mild conditions; the functional groups on the AuNP should react selectively with the incoming molecules; and the introduced molecules should require only little or no pre-modificalion steps before the installation.
[0077] The maleimide-tln'ol reaction is a popular reaction used by biochemists to effectively couple a peptide of interest to a substrate Moreover, as a variety of biomolecules are naturally bearing thiol or amino groups, a rnaleimide functionalized nanoparticle is a promising template for use as a carrier for drug delivery or therapeutic diagnostics
[0078] In an embodiment, the present specification relates to an economic, efficient and reproducible method for preparing stable, water-soluble, maleimide-terminated thiolate protected gold nanoparticles
[0079] In an embodiment, the present specification relates to the preparation of size controlled PEGylated gold nanoparticles with an average size ranging between about 2-10 nm, following a modified Brust method.'201 Subsequently, these PEGylated AuNPs were modified with a furan-protected maleimide-PEG-thiol. The resulting furan-protected maleimide-monolayer protected nanoparticle (MPN) has excellent thermal and temporal stability and the reactive maieimide group can be generated via a retro-Diels-Alder reaction simply by heating at 100°C over a period of two hours.
[0080] Ή-NMR spectroscopy represents a powerful tool to examine the structure and composition of the MPN monolayers.121,223 The resonance peaks of the MPN monolayers are characteristically broad mainly due to spin spin relaxation (T2) broadening. The dense packing of the Iigand chains close to the gold core results in a solid-like character due to dipolar interactions which translate into a fast spin relaxation. The short relaxation time results in a broadened and less intense signal. However, it is still possible to assign the peaks of the Ή-NMR spectrum of the MPN monolayers from their chemical shifts and gain important structural information. More importantly, the absence of sharp peaks is an important characteristic of the purity of the MPN monolayers.
[0081] The maleimide-NPs prepared in accordance wilh an embodiment of the present specification possess excellent stability and can be purified by washing and drying using regular organic work-up methods. 'H-NMR
spectroscopy was applied to monitor the retro- Diels-Alder reaction (for liberation of the maleimide) and was used to verify the purity of MPNs (FIG. 1 ).
[0082] Results demonstrated that the maleimide-NP can effectively react with amine or thiol model compounds via a Michael addition reaction which is indicative that the maleimide-NPs can be used as a platform to prepare; mono- modular or multi-modular probes by applying different combinations of thiol/amino containing probe molecules. Moreover, these maleimide-NPs can also be used as drug delivery vehicles by linking a drug to the maleimide group. In one embodiment, the drug comprises a thiol and/or amino function that ca be used for linking the drug to the maleimide NPs. In an embodiment, this druy- linking is accomplished using a thiol/amino containing linker. Furthermore, these maleimide-NPs can also be used as radioimmaging delivery vehicles by linking a radioimmaging agent or its precursor to the maleimide group.
[0083] The preparation of PEGylated MPN, in accordance with an embodiment of the present specification, is illustrated hereinbelow in Scheme 1.
r'E G-thiol
Scheme 1
[0084] The synthesis of maleimide-PEG thiol (6) and maleimide- functionalized gold NPs (Maleimide-AuNP) in accordance with an embodiment of the present specification is illustrated hereinbelow in Scheme 2.
Maleimide -A 11 NP
Scheme 2
[0085] The maleimide-functionalized gold NP could not be prepared by direct synthesis as a strong reducing reagent is usually applied during the reduction of Au(l) to Au(0). The desired maleimide-functionalized gold NP could also not be directly prepared via the commonly used place-exchange reaction with maleimide-PEG-thiol, because maleimide is a good thiol-scavenger and the maleimide-tethered thiol is likely to undergo a Michael addition reaction with itself. To circumvent these problems, a furan protected maleimide-PEG-thiol (6) was synthesized so that it could be place-exchanged onlo a PEGylated AuNP. The reactive maleimide group could then be generated by heating. Schemes 1 and 2 depict the modified Brust method used for the preparation of the PEGylated nanoparticle'701 (Scheme 1 ) and the preparation of the maleimide- functionalized gold NP (Scheme 2), respectively
[0086] In an embodiment of the present specification, triethylene glycol (n=2) was used.
[0087] In an embodiment of the present specification, the PEGylated
AuNPs were prepared as follows. Hydrogen tetrachloroaurate (HAuC ) or potassium tetrabromoaurate ( AuBr ) was dissolved in Milli-Q water and then mixed with tetraoctylammonium bromide (TOAB) in toluene. The contents were vigorously stirred in order to facilitate the phase transfer of the Au(lll) into the toluene layer. The organic layer was separated and dried with MgSd in order to remove any residual water and then cooled to 0'JC in an ice bath. Triethylene glycol-thiol "(TEG)-thiol", as a non-limiting example of a pegylated thiol, was added to the solution via a volumetric pipette and allowed to stir for about 10 minutes. The observed darK orange solution faded with time. When the thiol/gold molar ratio exceeded 2:1 , the solution became clear and colorless. Different sizes of AuNP can be obtained by varying the gold/thiol ratio. A fresh solution of tetrabutylammonium borohydride was then quickly added (in about 5
seconds) to a rapidly stirring toluene solution. The solution instantly turned black. The TEGylated NPs began to precipitate From the toluene after about 2 hours. After stirring the mixture overnight ("12 hours), 30 ml. of illi-Q water was added to extract the TEGylated AuNP. The TEGylated AuNPs were purified by washing with toluene/acetonitrile or dialysis. The resulting pure AuNP sample was assessed by 'H NMR spectroscopy, and showed no sign of free ligand. Unlike previously reported PEGylated NPs prepared from place- exchange reactions with citrate-nanoparticles or CTAB-nanoparticles, this TEGylated MPN has a smaller size, e.g. 3 nm, good stability, and could be easily prepared, purified, and characterized. It could also be repeatedly dried and readily redissolved in water.
[0088] The protected maleimide-PEG-thiol (6) was prepared as described hereinabove in Scheme 2. 3,6-Endoxo-A4-tetrahydrophthalimide (3) was reacted with excess dibromo-substituted PEG via a S reaction to generate the mono-substituted compound 4. The remaining bromide end-group of compound 4 was converted to the corresponding thioacetate 5 by reaction with KSAc/Acetone. Subsequent hydrolysis yielded the protected malcimide- PEG-thiol 6 in an overall yield of 30% (calculated starting from compound 3) The furan protected maleimide-PEG-thiol 6 was then incorporated into the previously prepared PEGylated AuNPs following a standard place-exchange reaction by mixing thiol 6/pegylated AuNP (mole ratio of 1 :1) over a period of one hour to generate the mixed ligand 6-AuNP. The purity of 6-AuNP, following a purification step, was confirmed by H-NMR spectroscopy (FIG. 1). The key resonances include the broad resonance at 6.35 ppm due to the alkene protons and those at 2.50 ppm due to the fused protons from the Diels-Alder adduct (FIG. 1a). The resonance of the methylene bridgehead protons was merged with the solvent peak while at room temperature. However, at an elevated
temperature of 90UC, the solvent peak shifts downfield and the signal at 4.95 ppm can be assigned to the methylene bridgehead protons (FIG. 1 )
[0089] The maleimide terminated gold nanoparfictes (maleimide-
AuNPs) were readily generated at elevated temperatures from the corresponding purified furan protected maleimide-MPN (6-AuNP). FIG. 1 illustrates the monitoring of the retro-Diels-Alder reaction by varied-temperature 1H-NMR spectroscopy. The broad peak at 5.82 ppm (indicated by the upward arrow; FIG. 1g) corresponds to the alkene protons of the maleimide wherwas the peaks at 7.45 ppm and 6 50 ppm are attributed to the free furan liberated from the retro-Diels-Alder reaction (the furan peaks eventually disappear after heating for 90 min). The maleimide end-group started to be produced while heating and was completely recovered following heating at 90 C over a period of one hour. The product ma!eimide-AuNPs were readily soluble in water following drying and could be re-dissolved for several cycles. The molar ra!io of maleimide PEG- thiolated ligand to PEGylated ligand was determined from the integration of the Ή-NMR spectrum of maleimide-AuNP to be 1 :2 (FIG. 6). TEM images of 6- AuNP before and after heating (maleimide-AuNP) showed a small increase of the core size and wider dispersion from 3.0 ± 0.5 nm to 3 2 ± 0 8 run (FIG. 2). This slight increase in core size was due to small quantities of ligand which were liberated as disulfide during heating.
[0090] Thermal gravimetric analysis (TGA) provided direct information on the quantity of organic components on the Au NP (FIG. 3). The total mass loss of the furan-masked AuNP (6-AuNP) was determined to be 8.6% Fur the maleimide-protected AuNP, the total mass loss was 8.0%. Ligands were removed from the gold NP surface starting at about 100°C. The additional mass loss for 6-AuNP accounts for the loss of furan from the retro Diels-Alder reaction.
[0091] Analysis of the data derived from TEM, NMR, and TGA experiments provided for an estimation of the composition of the AuNPs. Previous studies have indicated that the gold core has a truncated octahedron shape. ^ To simplify the calculation, it was assumed that the maleimide-AuNP has a spherical shape. The average diameter of the mateimide-AuNP core could be derived from the planar sphere projection (from TEM derived to be 3.2 nm). It was also assumed that the gold core has the same density as bulk gold, the average number of gold atoms per AuNP is thus ca. 1000, using the formula :l2 ]
[0092] N = ™~ N A
Ί·' , where
[0093] N = number of atoms per nanoparticle;
[0094] p = density of face centered cubic (fee) gold = 19.3g/cm \
[0095] d = average diameter of the nanoparticles;
[0096] MAi, = mole atomic weight of gold = 196.9665 g/mol;
[0097] NA = Avogadro constant
[0098] Thus the total number of ligands per gold nanoparticle can be calculated from the weight percentage of the organic portion of the PN In an embodiment of the present specification, the mixed ligand rnaleimide-AuNP is capped with two different ligands (e.g. maleimide-TEG-thiol ligands and
TEGylated thiol ligands), and the average protecting ligands number is determined to be 90, using:
[0099] N , - ω
M , , </? t M ( I - Ψ ) Where,
[00100] NL = total number of ligands per nanopartide;
[00101] ω = percentage of mass loss due to the protecting ligands;
[00102] MLi = molecular weight of the maleimide-TEG-thiol ligands
(CioH 14O4NS = 244.0643 g/mol);
[00103] Mi ? = molecular weight of the TEGylated thiol ligands
(C6Hi303S = 165.0585 g/mol);
[00104] φ = the molar percentage of the maleimide ligand.
[00105] The area per grafted ligand is thus 0.35 nm2, recognizing that this composition is based on using a gold core having a size of 3 2 nm The total mass of organic/gold particle was determined, providing for the determination of the relative contribution of the two organic species (ligands) to this total mass. This yielded a weighted average number of molecules per particle. Since the average surface area of the gold core is known, the area occupied per grafted ligand could be determined. An area of 0.35 nm"' is consistent with a moderately bulky thiol ligand. The simplified formula of IN: maleimide-AuNP can then be represented as Aui0on(TEG)Go( aleirnide)-io.
[00106] X-ray photoelectron spectra (XPS) of the maieimide AuNP confirm the presence of the maieimide group (FIG. 4). The nitrogen N 1 s BE value of 400.28 eV indicates the presence of the maieimide on the AuNP surface. The sulfur/nitrogen (S:N) ratio was 7:3, which was slightly lower than the calculated ratio of 9:3 (from the Ή-NMR integration). This discrepancy likely arises from an attenuation of the S signal relative to the N signal in the XPS experiment, given that the S is directly bonded to the gold surface and is thus buried relative to the N.
[00107] Unlike small organic molecules, Ihe reactivity of functional ligands on NPs might be quite different because of the steric environment of the NP ligands' corona. It was thus important to assess the reactivity and availability of the maieimide end groups of the maleimide-AuNPs. Maleimide-AuNPs were thus mixed with a large excess of furan (e.g. >2000 molar ratio) in aqueous solution over a period exceeding of a week. As expected, both exo- and endo- products were formed after the forward Diels-Alder reaction in a ratio of 5:4. This result confirmed that the maieimide end group in the maleimide-AuNP is freely accessible and reactive. Further modification of the maleimide-AuNP via Michael addition reaction would thus be facilitated.
[00108] EXPERIMENTAL
[00109] EXAMPLE 1
[00110] The reactive maieimide end groups of the maleimidc-AuNPs were shown to readily react with amines or thiols via the Michael addition reaction (Scheme 3). Cysteine modified biomolecules, such as for exam l cystein-derivatii ed oclreotate, represent a large class of bioprobe/precursors.
The model Michael addition reaction between a maleimide-AuNP and cysteine was studied by ^-l-NM spectroscopy. Although the new product peaks overlap with the resonance signal of the PEGylated ligands, the disappearance of the maleimide alkene proton peaks indicates that the Michael addition reaction proceeds under these conditions.
Scheme 3
[00111] EXAMPLE 2
[00112] The conjugation of the amino dye, rhodamine 123 (Scheme
3), was assessed by fluorescence spectroscopy which is more sensitive than NMR spectroscopy. Rhodamine 123 was mixed with maleimide-AuNP in an aqueous solution over a period of 1 h. The resulting rhodamine-AuNPs were purified by washing with an ethyl acetate:ethano! solvent mixture to remove any free rhodamine 123 until no fluorescence could be detected in the organic layer Because the fluorescence of this fluorophore-coupled NP was quenched by the
gold core (FIG. 5)|2R', cleavage of the ligand from the AuNP surface was necessary. Indeed, following I? cleavaget2r,), the fluorescence reappeared, indicating that the Michael addition reaction of Rhodamine 123 had occurred. Following cleavage from the gold AuNP, a mixture of compounds is obtained, comprising the 2 homodimers (i. e. the disulfide based on the rhodamine- maleimide-PEG-thiol ligand and the disulfide based on the PEG-thiol ligand) and the disulfide dimer based on both the rhodamine-maleimide-PEG-thiol ligand and PEG-thiol ligand.
[00113J EXAMPLE 3
[00114] The radiolabeling of a maleimide-AuNP with 1BF-labled 4-(di- fert-butylfluorosilanyl)benzenethiol ([18F]-SiFA-SH) for Position Emission Tomography (PET) was performed as follows (Fig. 7): A {d torf- Butylflurosilyl)benzenethiol (SiFA-SH) was prepared and [iaF]-SiFA-SH labeling was subsequently performed following a known literature procedure. w SiFA- SH was labeled via a fast isotope exchange reaction using [18F]F [16F]-SiFA- SH was then incorporated into the maleimide-AuNP via a Michael addition reaction. The resulting [1sF]-SiFA-AuNP was then purified through a ΝΛΡ-10 size exclusion column. Due to the characteristic black color of AuNPs, the pure [18F]-SiFA-AuNP fraction could be easily separated and collected in a radiochemical yield of 60%. Its purity was confirmed by radio-TLC A control labeling experiment was also carried out using [18F]-SiFA-OH and a maleimide- AuNP following the procedure illustrated in Fig. 7. Following separation of the ligand, there was no radioactivity observed on the AuNP which confirms that [18F]-SiFA-SH was attached to the NP surface via the desired Michael Addition reaction instead of physical adsorption.
[00115] EXAMPLE 4
[001 6] To evaluate the bio-distribution of the AuNPs, [,flF] SiFA AuNP
(12 MBq) were injected into healthy rats (n=3) and evaluated by micro-PET. Representative body and brain scan images of a micro-PET scan following intravenous injection of a solution of [,aF]-SiFA-AuNP are illustrated in Fig. 8. Radioactivity was found in the kidney, liver and spleen. One prominent insult from these studies was that this small [18F]-SiFA-AuNP was able to cross the blood brain barrier (BBB) as shown in Fig. 8d, e and f, even without specific modification of the AuNP surface with membrane penetrating peptides or proteins. Of note that [16F]-SiFA-SH alone does not cross the BBB.
[00117] The time-activity curves obtained from the PET images depicting the radioactivity uptake in the cerebellum and the brain (n=3), using bone radioactivity as a region of reference, are illustrated in Fig. 9 These curves illustrate that AuNPs start to accumulate in the cerebellum and brain at 25 - 120 min. Ex vivo biodistribution studies were also carried out to confirm the bio-distribution data of [1HF]-SiFA-AuNP in different organs 2 h following injection as obtained by micro-PET. The biodistribution data is illustrated in Fig. 10 and is expressed as the percentage of injected dose for each gram of tissue (%ID/g). The bio-distribution data were derived from an average of three rats (n=3) The bio-distribution results were in agreement with the results obtained from the PET images. The %lD/g value for brain and cerebellum is about 0.13%, about 50% higher than for bone uptake (0.08%), making the AuNP a promising deliveiy platform for the development of new PET tracers or radio-therapeutic agents. As there were no specific binding moieties conjugated to the AuNPs, the [1BFJ- SiFA-AuNP was homogenously distributed in the brain and no aggregation could be observed. The use of an ,8F-labeled AuNP for PET studies using a large NP (e.g. 13 nm) was recently reported.1281 However, the 1SF-Iabeled AuNP could not
be purified making an exact determination of its composition very difficult. Furthermore, larger AuNPs were reported as being unable to effectively cross the BBB.
[00118] The kidneys had the highest concentration of [i aF]-SiF'A-AuNP
(2%), indicative of a renal clearance of the [l 8FJSiFA-AuNPs (Fig. 10). Moreover, as the liver and spleen also had high concentrations of [i aF]-SiFA- AuNP (1.5%), respective hepatic and reticuloendothelial system (RES) clearances are also suggested. In view of the short half-life ot 10F { v = 110 min), a long term study of the clearance process was not performed.
[00119] [18F]-SiFA-SH was shown to effectively react with the water soluble maleimide-AuNPs of the present disclosure to generate radioactively labeled [ 8F]-SiFA-AuNPs. In an embodiment, these water soluble maleimide- AuNPs comprise a gold core having a size ranging from 3 to 4 nm. The labeled SiFA-AuNPs were readily purified by size exclusion column chromatography The radiolabeling was accomplished via a Michael addition reaction and no physical adsorption was observed when using [10F]-SiFA-OH which does not undergo Michael additions. The [18F]-SiFA-AuNPs were able to cross the blood brain barrier (BBB) as illustrated by small animal micro-PET studies as w l as by ex vivo biodistribution data.
[00120] EXAMPLE 5
[00121] Radiosynthesls of [18F]-SiFA-SH: [i enfluoride/Ha[, B0]0 (30 mCi) was passed through a QMA cartridge preconditioned with 10 mL of 0.5M K2C03 and 10 mL of HaO. The trapped 18F was then eluted with 1 mL of a stock solution containing 75 mg (0.20 mmol) K.ryptofix2.2.2 and 0.10 rnrnol potassium
oxalate in 10 mL of 96/4 acetonitriie/H20 solution. The residual water was azeotropically dried with anhydrous acetonitrile (MeCN) at 105UC. [lflF]F cryptate was dissolved in 0.3 mL of anhydrous MeCN. SiFA-SH (40 pg, 148 nmol) in 0 2 mL of dry MeCN was added and reacted at room temperature for 10 min without stirring. This [18F]SiFA-SH/MeCN (20 mCi) solution was used for Maleimide-AuNP conjugation without further purification
[00122] Reaction of [1sF]-SiFA-SH with Nlaleimitie-AuNP: 2 mL of the stock Maleimide-AuNP solution (55 mg in 25rnL water) was lyophili ed and re-dissolved in 1 mL of phosphate buffer (0.1 M, pH 7.2). The muleimide reactive part was recovered by heating the solution at 100°C for 2h. To this solution was subsequently added [18F]SiFA-SH (20 mCi; obtained as described above) and reacted al room temperature for 10 min without stirring. The 18F- labeled AuNP was purified using a Nap-10 column (equilibration buffer 0 1 M PBS, pH 7.2). The black colored fraction was collected and the purity of the product was confirmed by TLC. The radiochemical yield was approximately 60% (12 mCi).
[00123] Biod is tri button of [18F]-AuNP in Rats by small animal PET;
SD (Sprague-Dawley) Rats for in vivo PET studies were housed in a 12h lighl dark cycle at 21UC with access to food and water ad libitum. Treatment was in accordance with the Guide to the Care and Use of Experimental Animals (Ed2) of the Canadian Council on Animal Care. The Micro-PET imaging protocol was approved by the Animal Care Committee of McGill University (Montreal, Canada). The rats were kept under isoflurane anaesthesia for the injection of the radiotracer. Respiration rate, heart rate and body temperature were monitored throughout the scan (Biopac sytems MP150, Goleta, CA, USA). 12-15 MBq of [18F]-AuNP were intravenously administered via the lateral tail vein. Whole body data were acquired for 1 h and brain data were acquired for 2h
using a Concord MicroPET R4 small animal tomograph. All images were reconstructed using filtered back projection after applying normalized scatter correction for attenuation and radioactive decay. The PET images were analyzed using ASIPRO software (Concorde Microsystems). The time activity curves were obtained from regions of interest in heart and brain using bone and muscle as the references.
[00124] The SD rats were euthanized 2h post-injection of the radiotracers. The organs were removed, rinsed with physiological saline, blotted dry and placed in pre-weighted tubes. The radioactivity in each tube was counted in a gamma counter (Cobra II, Packard Instruments) and used to calculate the percentage of the injected dose per gram of tissue (% ID/g) for each organ. The means from three rats are reported.
[00125] It is to be understood that the specification is not limited in its application to the details of construction and parts as described hereinabove. The specification is capable of other embodiments and of being practiced in various ways. It is also understood that the phraseology or terminology used herein is for the purpose of description and not limitation. Hence, although the present invention has been described hereinabove by way of illustrative embodiments thereof, it can be modified, without departing from the spirit, scope and nature of the subject disclosure as defined in the appended claims.
References
1. Cheort, J.; Lee, J. Acc Chem. Res. , 2008, 41, 1630-1640.
2. Daniel, M. C; Astruc, D. Chem. Rev., 2004, 104, 293-346.
3. (a) Rosi, N. L; Mirkin, C. A. Chem. Rev., 2005, 105. 1547-1562. (b) Rosi, N L; Giljohann, D. A.; Thaxton, C. S.; Lytton-Jean, A. K. R. ; Han, M. S , and Mirkin. C. A. Science, 2006, 372, 1027-1030.
4. (a) Sonnichsen, C; Reinhard, 6. M.; Liphardt, J.; Alivisatos, A P., Nature Biotechnology, 2005, 23, 741 -745. (b) Reinhard, B M.; Siu, M.; Agarwal, H.; Alivisatos, A. P. ; Liphardt, J., Nano Letters, 2005, 5, 2246-2252.
5. El-Sayed, I. H. , Huang, X.; and El-Sayed, M. A, Nano Lett, 2005, 5. 829- 834.
6. Turkevich, J ; Stevenson, P. C; and Hillier, J. Discuss Faraday Soc . 1951 ,
11, 55-75.
7. Frens, G. Nature: Rhys. Sci., 1973, 247 , 20-22.
5. (a) Lo, C. K.; Xiao, D,; and Choi, M. M. F. J. Mater. Chem., 2007, 17, 2418- 2427. (b) Patil, V.; Mayya, K. S.; and Sastry, M. Langmuir, 1999, 15, 6587- 6590. (c) Yonezawa, T ; Kunitake, T. Colloid Surf. A.: Physicochem Fny Asp. 1999, 149, 193-199.
9. Templeton, A. C; Chen, $.; Gross, $. M. ; and Murray, R. W. Langmuir,
1999, 15, 66-76.
10. Owens, D. E. Ill; and Peppas, N. A Int. J. Pharm., 2006, 307, 93-102.
11. Simpson, C. A.; Huffman, B. J. ; Gerdon, A. E.; and Cliffel, D. A. Chem. Res.
Toxicol, 2010, 23, 1608-1616.
12. Liu, Y.; Shipton, M. K.; Ryan, J.; Kaufman, E. D.; Franzen, S.; and Feldheim, D. L. Anal. Chem., 2007, 79, 2221-2229
13. Niidome, T ; Yamagata, M.; Okamoto, Y.; Akiyama, Y.; Takhashi, H., Kawano, T.; Katayama, Y.; and Niidome, Y. J Controlled Release, 2006, 114, 343-347.
14. Sonavane, G.; Tomoda, K ; Sano, A.; Ohshima, H.; Terada, H. ; and akino, K. Colloids Surf. B, 2008, 65, 1-10.
15. US Patent No. 5,521 ,289.
16. Gregori, L.; Hainfeld, J. F.; Simon, M. N.; Goldgaber, D. J. Biol. Chem , 1997,
272, 58-62.
17. Zhu. J.; Lines, B. M.; Ganton, M. D.; Kerr, M. A.; and Workentin, M. S. J Org.
Chem., 2008, 73, 1099-1105. (b) Zhu, J.; Ganton, M. D.; Kerr, M. A.: and
Workentin, M. S. J. Am. Chem Soc . 2007, -729, 4904-4905. (c) Zhu, J., Kell, A. J.; and Workentin, M. S. Org. Lett., 2006, 8, 4993-4996.
Ba, H.; Rodriguez-Fernandez, J.; Stefani, F. D.; and Feldmann, J Nana Letters, 2010, 10, 3006-3012.
0h, E.; Susumu, .; Blanco-Canosa, J. B.; edintz, I. L,; Dawson, P. E.; and Mattoussi H. Small, 2010, 6, 1273-1278.
Brust, M.; Walker, M.; Bethell, D. ; Schiffrin, D. J.; and Whyman, R. J. J. Chem. Soc. Chem. Commun. , 1994. 801 -802.
(a) Badia, A; Cuccia, L; Demers, L; Morin, F; Lennox, R. 6.; and Reven, L. J. Am. Chem. Soc, 1997, 119, 1 1104-1 1105. (b) Badia, A ; Singh, S.; Demers, L,; Cuccia, L.; Brown, G. R. ; and Lennox, R. B. Chem. Eur J , 1996, 2, 359-363. (c) Badia, A.; Lennox, R. B.; Reven, L. Acc. Chem. Res. 2000, 33, 475-481.
(a) Terrill, R.H.; Postlethwaite, T. A.; Chen, C. H.; Poon, C. D.; Terzis, A.; Chen, A.D.; Hutchison, J. E.; Clark, M R.; Wignall, G.; Londono, J.D.; Superfine, R.; Falvo, , ; Johnson, C S., Samulski, E. T ; and Murray R. W. J. Am. Chem. Soc, 1995, 117, 12537-12548. (b) Templeton, A. C: Wuelfing, W. P. ; Murray, R. W. Acc. Chem. Res., 2000, 33, 27-36.
(a) Whetten, R. L; Khoury, J. T., Alvarez, M. M.; Murthy. S ; Ve7mar, I ; Wang, Z.; Stephens, P. W. Adv. Mater., 1996, 8, 428-433. (b) Cleveland. C. L; Landman, U.; Schaaff, T. G. ; Shaftgullin, M. N.. Stephens, P. W.; Whetten, R. L. Rhys. Rev. Lett. 1997, 79, 1873-1876. (c) Whetten, K. L.; Shafigullin, M. N.; Khoury, J. T.; Schaaff, T. G.; Vezmar, I.; Alvarez, M. M. ; Wilkinson, A. Acc. Chem. Res., 1999, 32, 397-406.
Liu, X., Atwater, M., Wang, J„ and Huo, Q Colloids Surf B, 2007, 58, 3-7. (a) Mayilo, S.; Kloster, M. A.; Wunderlich, M.; Lutich, A.; Klar, T.A.; Nichtl, A. ; KUrzinger, K.; Stefani, F. D.; and Feldmann, J. Nano Lett. 2009, 9, 94558- 4563. (b) Schneider, G.; and Decher, G. Nano Lett , 2006, 6. 530-536 (c) Hong, R.; Fernandez, J. M.; Nakade, H.; Arvizo, R ; Emrick, T.; and Rotello, V. M. Chem. Commun., 2006, 2347-2349.
Templeton, A. C ; Hostetler, M. J.; Kraft, C. J.; Murray, R. W. Am Chem SOC , 1998, 120, 1906-191 1
Wangler, B ; Quandt, G.; lovkova, L.; Schirrmacher, E.; Wangler, C; Boenig, G.; Hacker, M,; Schmoeckel, M.; Jurkschat, K.; Bartenstein, P. , and Schirrmacher, R. Biocorijugate Chemistry, 2009, 20, 317-321 .
Guerrero, S.; Herance, J. R.; Rojas, S.; Mena, J. F.; Gispert, J. D. , Acosla, G. A.; Albericio, F.; Kogan, M. J. Bioconjugate Chemistry, 2012, 23, 399 408.
Claims
1. A maieimide-functionalized gold nanoparlicle comprising a ligand monolayer, wherein said ligand monolayer includes at least a P G- thiolated ligand.
2. The maieimide-functionalized gold nanoparticle of claim 1 having the Formula I;
3. The maieimide-functionalized gold nanoparticle of claim 1 having the Formula II:
4. A maieimide-functionalized gold nanoparti obased bioprobe of Formula III;
"n" and "m" are integers independently ranging from 1 to 100,
X is selected from the group consisting of NR1 and S;
R1 is H or Ci-salkyl;
B is a probe, a therapeutic agent or a radioimmaging agon I or its precursor; and
"z" is an integer ranging from 0 to 5
5. The maleimide-functionalized gold nanoparticlc-based bioprobe of claim 4, wherein the probe is a biomolecular probe.
6. The maleimide-functionalized gold nanoparticle of claim 1 comprising from about 950 to about 2300 gold atoms.
7. The maleimide-functionalized gold nanoparticle of claim 6 comprising about 1000 gold atoms.
8. The maleimide-functionalized gold nanoparticle ol claim 1 comprising a gold core having an average size ranging from about 3 to 4 nm.
9. The maleimide-functionalized gold nanoparticle of claim 8 comprising a gold core having an average size of about 3.2 nrn.
10. The maleimide-functionalized gold nanoparticle of claim 2 wherein the maleimide-terminated ligands and PEG ligands are present in a ratio ranging from 1 :1 to 1 :3
11. The maleimide-functionalized gold nanoparticle of claim 1 wherein the gold nanoparticle is water-soluble.
12. A process for preparing a maleimide-functionalized gold nanoparticle in accordance with any one of claims 1 to 4, the process comprising reacting a PEGylated AuNP with a furan-protected maleirnide-PEG-thiol under conditions suitable to provide the maleimide-functionalized gold nanoparticle in accordance with any one of claims 1 to 4.
13. A process for preparing a maleimide-functionalized gold nanoparticle of claim 2, the process comprising reacting a PEGylated AuNP with a furan-protected maleimide-PEG-thiol to produce a furan-protected maleimide- functionali2ed gold nanoparticle of Formula I I , followed by heating the furan- protected maleimide-functionalized gold nanoparticle of Formula II under conditions suitable for removal of the furan-protection group and to provide the maleimide-functionalized gold nanoparticle of claim 2.
14. A kit comprising a maleimide-functionalized gold nanoparticle in accordance with any one of claims 1 to 4.
15. Use of the maleimide-functionalized gold nanoparticle of claim 4 for multimodal biological applications.
1Θ. A method for delivering a therapeutic agent to a subject, the method comprising:
a) providing a nanoparticle of Formula I;
b) coupling the therapeutic agent to the nanoparticle of Formula I to form a nanoparticle-drug conjugate; and
c) administering to the subject the nanoparticle-drug conjugate
17. A method for delivering a radioimmaging agent to a subject, the method comprising:
a) providing a nanoparticle of Formula I;
b) coupling the radioimmaging agent to the nanoparticle of Formula I to form a nanoparticle- radioimmaging agent conjugate; and
c) administering to the subject the nanoparticle-radioimmaging agent conjugate.
18. A radiolabeled maleimtde-funclionalized gold nanoparticle of
Formula IV
19. Use of the radiolabeled rnaleimide-functiona zed gold nanoparticle of claim 16 as a radiotracer in spectroscopic imaging applications
20. The use of claim 19, wherein the imaging application is
PET.
21. Use of the maleimide-functionalized gold nanoparticle of claim 2 as a carrier for PET tracers or therapeutic agents.
22. A method of using the radiolabeled maleimide- functionalized gold nanoparticle of Formula IV as a radiotracer for radioimaging applications, the method comprising administering the radiolabeled fnaleimide- functionalized gold nanoparticle of Formula IV to a subject.
23. The method of claim 22, wherein the radioimaging application is PET.
A furan protected maleimide-PEG-thiol having the formula
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/122,505 US20140186263A1 (en) | 2011-05-31 | 2012-05-31 | Maleimide-functionalized gold nanoparticles |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161491638P | 2011-05-31 | 2011-05-31 | |
US61/491,638 | 2011-05-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2012162820A1 true WO2012162820A1 (en) | 2012-12-06 |
Family
ID=47258229
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CA2012/000609 WO2012162820A1 (en) | 2011-05-31 | 2012-05-31 | Maleimide-functionalized gold nanoparticles |
Country Status (2)
Country | Link |
---|---|
US (1) | US20140186263A1 (en) |
WO (1) | WO2012162820A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108129652A (en) * | 2017-12-25 | 2018-06-08 | 湖南华腾制药有限公司 | A kind of polyethylene glycol lysine maleimide thioguanine conjugate |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2016128988A1 (en) | 2015-02-10 | 2016-08-18 | Rajiv Gandhi Institute Of Petroleum Technology, Rae Bareli | A process for the preparation of polyacryloyl hydrazide stabilized metal nano particles and the products obtained thereby |
WO2016196783A1 (en) * | 2015-06-04 | 2016-12-08 | The Regents Of The University Of California | Combination of isolated individual enhancements of x-ray radiation effect by nanomaterials |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2650008A1 (en) * | 2006-04-25 | 2007-11-01 | Centre National De La Recherche Scientifique (Cnrs) | Functionalization of metal nanoparticles with oriented proteins |
US20080226995A1 (en) * | 2007-03-13 | 2008-09-18 | Costanzo Philip J | Thermally controlled particulate core migration within polymer matrix |
WO2012025646A1 (en) * | 2010-08-27 | 2012-03-01 | Universitat De Barcelona | Maleimide‑furanyl compounds that can be used in a general method for preparing maleimide‑oligonucleotide derivatives |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7229841B2 (en) * | 2001-04-30 | 2007-06-12 | Cytimmune Sciences, Inc. | Colloidal metal compositions and methods |
US7413770B2 (en) * | 2002-08-01 | 2008-08-19 | E.I. Du Pont De Nemours And Company | Ethylene glycol monolayer protected nanoparticles |
US20060222595A1 (en) * | 2005-03-31 | 2006-10-05 | Priyabrata Mukherjee | Nanoparticles for therapeutic and diagnostic applications |
-
2012
- 2012-05-31 WO PCT/CA2012/000609 patent/WO2012162820A1/en active Application Filing
- 2012-05-31 US US14/122,505 patent/US20140186263A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2650008A1 (en) * | 2006-04-25 | 2007-11-01 | Centre National De La Recherche Scientifique (Cnrs) | Functionalization of metal nanoparticles with oriented proteins |
US20080226995A1 (en) * | 2007-03-13 | 2008-09-18 | Costanzo Philip J | Thermally controlled particulate core migration within polymer matrix |
WO2012025646A1 (en) * | 2010-08-27 | 2012-03-01 | Universitat De Barcelona | Maleimide‑furanyl compounds that can be used in a general method for preparing maleimide‑oligonucleotide derivatives |
Non-Patent Citations (2)
Title |
---|
OLIVER, J.-C. ET AL.: "Svnthesis of Peylated Immunoparticles", PHARMACEUTICAL RESEARCH, vol. 19, no. 8, 2002, pages 1J137 - 1143 * |
ZHU, J ET AL.: "Preparation of Water-Soluble Maleimide-Functionalized 3 nm Gold Nanoparticles: A New Bioconjugation Template", LANGMUIR, vol. 28, 19 March 2012 (2012-03-19), pages 5508 - 5512 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108129652A (en) * | 2017-12-25 | 2018-06-08 | 湖南华腾制药有限公司 | A kind of polyethylene glycol lysine maleimide thioguanine conjugate |
CN108129652B (en) * | 2017-12-25 | 2020-04-07 | 湖南华腾制药有限公司 | Polyethylene glycol lysine maleimide thioguanine conjugate |
Also Published As
Publication number | Publication date |
---|---|
US20140186263A1 (en) | 2014-07-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Glaus et al. | In vivo evaluation of 64Cu-labeled magnetic nanoparticles as a dual-modality PET/MR imaging agent | |
Reichert et al. | Metal complexes as diagnostic tools | |
Mitchell et al. | Incorporation of paramagnetic, fluorescent and PET/SPECT contrast agents into liposomes for multimodal imaging | |
Huang et al. | Biodegradable polydisulfide dendrimer nanoclusters as MRI contrast agents | |
EP2791254B1 (en) | Functionalised silicon nanoparticles | |
EP4000640A1 (en) | Dual mode radiotracer and therapeutic | |
EP3354375A1 (en) | Nanomaterial and method of production of a nanomaterial for medical applications, such as mri or sers | |
Tamba et al. | Silica nanoparticles: preparation, characterization and in vitro/in vivo biodistribution studies | |
JP2011500652A (en) | Use of lanthanide-based nanoparticles as radiosensitizers | |
Helbok et al. | Targeting properties of peptide-modified radiolabeled liposomal nanoparticles | |
Srivatsan et al. | Recent advances in nanoparticle-based nuclear imaging of cancers | |
Caminade et al. | Dendritic metal complexes for bioimaging. Recent advances | |
US10786582B2 (en) | Gd(III)-dithiolane gold nanoparticle conjugates | |
Grallert et al. | Polymeric micelles and molecular modeling applied to the development of radiopharmaceuticals | |
Laznickova et al. | Mono (pyridine-N-oxide) DOTA analog and its G1/G4-PAMAM dendrimer conjugates labeled with 177Lu: radiolabeling and biodistribution studies | |
US20150004096A1 (en) | Tumorspecific SPECT/MR(T1), SPECT/MR(T2) and SPECT/CT contrast agents | |
Lane et al. | 99mTc (CO) 3-DTMA bombesin conjugates having high affinity for the GRP receptor | |
WO2012162820A1 (en) | Maleimide-functionalized gold nanoparticles | |
MXPA06006044A (en) | Contrast agents. | |
Zhu et al. | Charge-conversional polyethylenimine-entrapped gold nanoparticles with 131 I-labeling for enhanced dual mode SPECT/CT imaging and radiotherapy of tumors | |
Fontes et al. | Ga (III) chelates of amphiphilic DOTA-based ligands: synthetic route and in vitro and in vivo studies | |
Prasanphanich et al. | The effects of linking substituents on the in vivo behavior of site-directed, peptide-based, diagnostic radiopharmaceuticals | |
Xu et al. | A novel, chelator-free method for 64 Cu labeling of dendrimers | |
Class et al. | Patent application title: MALEIMIDE-FUNCTIONALIZED GOLD NANOPARTICLES Inventors: Ralf Schirrmacher (Montreal, CA) Jun Zhu (Montreal, CA) R. Bruce Lennox (Montreal, CA) Assignees: The Royal Institution for the Advancement of Learning/McGill University | |
Gonçalves et al. | Studies on the biodistribution of dextrin nanoparticles |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 12793402 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 14122505 Country of ref document: US |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 12793402 Country of ref document: EP Kind code of ref document: A1 |