WO2012133633A1 - Disposable lysine sensor - Google Patents

Disposable lysine sensor Download PDF

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Publication number
WO2012133633A1
WO2012133633A1 PCT/JP2012/058334 JP2012058334W WO2012133633A1 WO 2012133633 A1 WO2012133633 A1 WO 2012133633A1 JP 2012058334 W JP2012058334 W JP 2012058334W WO 2012133633 A1 WO2012133633 A1 WO 2012133633A1
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Prior art keywords
lysine
disposable
oxidase
electrode
measuring electrode
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PCT/JP2012/058334
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French (fr)
Japanese (ja)
Inventor
武司 上村
希 稲葉
加成恵 板岡
悦夫 篠原
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株式会社テクノメデイカ
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Priority to JP2011-071654 priority
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Publication of WO2012133633A1 publication Critical patent/WO2012133633A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806Determination of free amino acids
    • G01N33/6812Assays for specific amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/902Oxidoreductases (1.)
    • G01N2333/906Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02Food

Abstract

[Problem] To provide a disposable sensor capable of measuring lysine. [Solution] The disposable lysine sensor relating to the present invention is a sensor for measuring the matrix concentration of a sample solution by forming an electrode system having at least a measuring electrode and a counter electrode on an electrically insulating substrate and using the electrode system to electrochemically detect changes in the substance concentration during reaction of an enzyme, electron receptor and sample solution, which is characterized by the formation, on the measuring electrode, of a reaction layer that supports lysine oxidase and ferricyan ions, which react with lysine in the presence of the lysine oxidase as a catalyst.

Description

Disposable lysine sensor

The present invention relates to a disposable sensor for measuring the lysine.

In recent years, the malnutrition despite are taking a meal, so-called, is the patient to become a modern type malnutrition is increasing.
The modern type malnutrition, refers to the malnutrition caused by spite to are taking a certain amount of meal, the components necessary for the body from the bias of the meal content is depleted.
In modern times that can eat freely choose what you like, the patient to become a modern type malnutrition while there is no subjective symptoms the person by the gastronomic and unbalanced diet often.
The components needed for the body, often there, but play a role protein to build muscle, organs, bone, skin, blood, hormones, enzymes, such as an immune substance is particularly important.
Proteins, macromolecules 20 amino acids are able to bind the human body is composed of about 100,000 different proteins. Said of the amino acid, because the 9 types can not be synthesized in the body, must be ingested from food, these 9 kinds of amino acids called essential amino acids.

JP 10-197473 Laid

Lysine, which is one of the essential amino acids, and repair the body's tissues, is an important amino acid involved in the body of the growth, which may cause growth failure or the like to be deficient.
Lysine as described above, regardless of whether it is a very important amino acid, most likely to lack in essential amino acids.
Lysine, not included much in vegetable protein, and dairy products such as milk and cheese, soy, meat, because it contains abundant in fish, by the wrong diet and unbalanced diet, etc., these foods If you do not intake causes a shortage in the body, and as a result, leading to the modern type malnutrition.
Lysine as described above, despite an important item in diagnosing modern type malnutrition, diagnosis of malnutrition, it is common to be performed by examining the amount of albumin, malnutrition does not measure lysine is carried out for the diagnosis of.
Conventionally, instead of using the enzyme as an electron acceptor, the biosensor using a metal complex or an organic compound such as potassium ferricyanide as the electron acceptor has been proposed, in this type of biosensor, substrate and enzyme to be measured by oxidation at the electrode reductant of the electron acceptor generated by enzyme reaction between, but seeking substrate concentrations from the oxidation current value (see Patent Document 1), even in the biosensor of this kind, biosensor for the measurement of lysine is not present.
Inventors have focused on current situation described above, conducted intensive studies on the biosensor capable of measuring the lysine found a combination of optimal lysine oxidase and an electron acceptor to measure lysine in disposable sensor .
An object of the present invention is to provide a disposable sensor that can measure lysine.

Disposable lysine sensor according to the present invention in order to achieve the above object, an electrode system is formed having at least a measuring electrode and a counter electrode on an electrically insulating substrate, enzyme, during the reaction of the electron acceptor and the sample solution holding the sensor detects the material density changes electrochemically the electrode system for measuring the substrate concentration of the sample solution, the measurement electrode, lysine oxidase and ferricyanide ions which react with lysine as a catalyst the lysine oxidase characterized in that the formation of the reaction layer.
The lysine oxidase, for example, can consist of fungal origin enzymes, specifically, an enzyme derived from cells of Trichoderma, more specifically, may be an enzyme derived from Trichoderma last place de bacteria.
Also, a second measuring electrode provided on the electrically insulating substrate, even when the lysine oxidase said second measuring electrode to form a reaction layer containing no lysine oxidase holding the ferricyanide ions which react with lysine as a catalyst good.
Furthermore, forming the second measuring electrode on an electrically insulating substrate provided, the reaction layer holding the ferricyanide ions which react with lysine said second measuring electrode lysine oxidase activity without protein and lysine oxidase as a catalyst it may be. In this case, the protein without the lysine oxidase activity can be, for example, lysine oxidase was inactivated.

Disposable lysine sensor according to the present invention, the electrically insulating substrate on which an electrode system having at least a measuring electrode and a counter electrode, an enzyme, a substance concentration change during the reaction of the electron acceptor and the sample solution is electrochemically in the sensor for measuring the substrate concentration detected by the electrode system the sample solution, the measurement electrode, to form a reaction layer which holds the ferricyanide ions which react with lysine lysine oxidase, and the lysine oxidase as a catalyst since, it is possible to easily measure the lysine. Thus, even in the diagnosis in general medicine and the like, it becomes possible to measure the lysine, it is possible to diagnose more accurately malnutrition.
The inventors have, as the lysine oxidase, fungal-derived enzyme, specifically, enzymes from bacterial Trichoderma, more specifically, using the enzyme derived from Trichoderma kink de bacteria, and, electron acceptor by using ferricyanide ions as the body, it was confirmed that favorable results are obtained.
Also, a second measuring electrode provided on the electrically insulating substrate, by a lysine oxidase said second measuring electrode to form a reaction layer containing no lysine oxidase holding the ferricyanide ions which react with lysine as a catalyst since it is possible to measure the amount of lysine based on the measurement result of the second measuring electrode without the first measuring electrode and lysine oxidase containing lysine oxidase, thereby enabling more accurate measurement.
Also, a second measuring electrode provided on the electrically insulating substrate, Felicia that reacts with lysine said second measuring electrode without lysine oxidase activity protein (e.g., lysine oxidase was inactivated) and lysine oxidase as a catalyst by forming the reaction layer holding the N'ion, it is possible to more closely the conditions of the first measuring electrode and the second measuring electrode, it is possible to more accurate measurements.

Is a view showing the measurement principle of a disposable lysine sensor according to the present invention. It is a graph showing a cyclic voltammogram obtained by cyclic voltammetry using a ferricyanide ion and molds from lysine oxidase. (A) is a graph showing a change in current value when done with the addition of lysine sequentially, (b) is a graph showing a current value according to the density change due to the addition of lysine. In FIG. 3 (b), is a graph showing the lysine low density side. It is a schematic exploded view of a disposable lysine sensor according to the present invention. (A) ~ (e) are schematic views showing a method of manufacturing disposable lysine sensor. (A) is a schematic top view of a disposable lysine sensor and portable analyzer is a schematic top view showing a (b) is a state of wearing the disposable lysine sensor portable analyzer. The internal processing of the portable analyzer is a block diagram schematically showing.

Hereinafter, it will be described the embodiments of the disposable lysine sensor according to the present invention with reference to the accompanying drawings.

Figure 1 is a view showing the measurement principle of a disposable lysine sensor according to the present invention.
As shown in the drawings, the disposable lysine sensor according to the present invention, a lysine oxidase, is used and the ferricyanide ion as the electron acceptor. The lysine as a substrate contained in the blood is oxidized by lysine oxidase, via a lysine oxidase by moving electrons to ferricyanide ions as electron acceptor, ferrocyanide ions ferricyanide ion is reduced generate, measure the concentration of lysine based on oxidation response current flowing when oxidized by the working electrode and ferrocyanide ions.

Measurement of lysine (Fig. 2)
Used equipment:
Potentiometer, galvano-stat (HA-501) (manufactured by Hokuto Denko Co., Ltd.)
Function Generator (HB-104) (manufactured by Hokuto Electric Works Co., Ltd.)
Measurement condition:
- counter, the concentration of the use-lysine working electrode both platinum wire 5 [mu] mol / dL
· Ferricyanide ion concentration is 5mM
And potential scanning range -300 ~ 800mV
· Scanning rate 50 mV / s
And environmental temperature 28 ℃
2, curve a, depositing a ferricyanide ion concentration of 5mM on the working electrode, there potential (V) and current density upon addition of lysine solution at a concentration of 5 [mu] mol / dL of (A / cm2) It shows the relationship.
2, curve b, the potential at which deposition of the ferricyanide ion concentration of 5mM to the working electrode, and thereto added lysine oxidase from fungi lysine solution and 1U / ml concentration of a concentration of 5 [mu] mol / dL (V) and it shows the relationship between the current density (a / cm2). Here, (EC number of Sigma-Aldrich Japan Co., Ltd. Sales: 1.4.3.14) Trichoderma Billiton de lysine oxidase derived from bacteria is an enzyme derived from fungi as lysine oxidase was used.
From the curves a and b, and the reaction proceeds, ferrocyanide ions ferricyanide ions are consumed is generated near the electrode, by adding it to lysine oxidase, ferricyanide ions functions as a mediator of lysine oxidase, further it can be confirmed that the current value of the oxidation side is increased because ferrocyanide ions are generated. Thus it was possible to confirm the redox peak attributable to ferricyanide ions in the system of lysine as with glucose system. From this result, it was confirmed that the ferricyanide ion is functioning as an electron acceptor lysine oxidase.
The curve c and d are not dependent on ferricyanide ion respectively show the cyclic voltammograms according to (hydrogen peroxide by) reaction. From the curves c and d, the hydrogen peroxide generated in this reaction can be confirmed that little influence on the current value of the oxidation side.

Then, the reaction system lysine and lysine oxidase, according to increase the lysine concentration, shows the results of what current response experiments were performed to see if seen in Figure 3 (a) and (b).
The measurement conditions are as follows.
Used equipment:
Potentiometer, galvano-stat (HA-501) (manufactured by Hokuto Denko Co., Ltd.)
Function Generator (HB-104) (manufactured by Hokuto Electric Works Co., Ltd.)
Measurement condition:
- counter to gold plate, using lysine oxidase deposited plate gold working electrode 22.4μl adding and ferricyanide ion concentration 5mM
And potential scanning range 550mV
And environmental temperature 28 ℃
Depositing a ferricyanide ion concentration of 5mM to the working electrode, together with the addition of lysine oxidase 22.4μl there was added a lysine solution as shown in Table 1. Here, (EC number of Sigma-Aldrich Japan Co., Ltd. Sales: 1.4.3.14) Trichoderma Billiton de lysine oxidase derived from bacteria is an enzyme derived from fungi as lysine oxidase was used.

Figure JPOXMLDOC01-appb-T000001
The change in the current value in FIG. 3 (a) when done with the addition of lysine sequentially, FIG. 3 (b) shows the current value according to the density change due to the addition of lysine.
As shown in FIG. 3 (a) and (b), in accordance with gradually increasing the amount of lysine, the current value in a stepwise manner, it was confirmed that the increase.
4, in the test results shown in FIG. 3 (b), a graph enlarging the following 0.99 nmol / ml to assume a practical concentration region.
As shown in the test results of FIG. 3 and FIG. 4, in accordance with gradually increasing the amount of lysine, since the current value in a stepwise manner is gradually increased, the current response by reaction of lysine and the lysine oxidase was confirmed. The current value, it was confirmed to correlate with lysine amount.

Next, it will be described for one embodiment of a disposable lysine sensor according to the measurement principle described above.
Figure 5 is a schematic exploded view of a disposable lysine sensor according to the present invention, FIG. 6 (a) ~ (e) are schematic views showing a manufacturing method of disposable lysine sensor.
Reference numeral 1 denotes an insulating substrate. On this substrate 1, after printing by the semiconductor printing technology gold, first measuring electrode 2 by heating drying, a second measuring electrode 3 is formed, also a silver / silver chloride is printed by a semiconductor printing technique after forming the counter electrode 4 by heat drying. Wherein the first measuring electrode 2 and the second measuring electrode 3 is formed in a position that is symmetrical about the counter electrode 4. (Figure 6 (a)).
Next, the first measuring electrode 2 of the substrate 1, the second measuring electrode 3 and counter electrode 4 of the electrode portion 2a, 3a, 4a and the terminal portions 2b, 3b, the photoresist film 8 so as to cover portions other than 4b provided (Figure 6 (b)).
Then, on the first measuring electrode and second electrodes 2a, lysine oxidase, was coated dry surfactant and ferricyanide ion dissolved polymer solution, a first reaction layer 6 on the first measuring electrode 2 electrode 2a Form. At the same time, on the second measuring electrode 3 electrode 3a, it was coated dry the same amount of polymer solution of the first reaction layer 6 surfactant and ferricyanide ions in the same amount, the second measuring electrode 3 electrode the second reaction layer 7 is formed on the 3a (Figure 6 (c)).
Then, the cover on the photoresist layer 8, the spacer 9 that notch 9a is provided to form a sample flow path 12 is disposed (FIG. 6 (d)), further thereon, the air holes 10 are formed 11 disposable lysine sensor is completed by providing a (FIG. 6 (e)).
Notch 9a of the spacer 9 surrounds the air hole 10 of the first measuring electrode 2, the second measuring electrode 3 and counter electrode 4 of the electrode portion 2a, 3a, 4a and the cover 11, and disposed on the substrate 1 end located on the opposite side of the terminal portions 2b, 3b and 4b are formed so as to form a sample introduction port 13 is opened in the state. The thickness of the spacer 9 between the photoresist film 8 and the cover 11, the thickness to form a specimen flow path 12 of the height to cause capillary action of the sample, specifically, for example, of approximately 0.3mm it is the thickness.
With the configuration described above, when the user introduces a sample such as blood from a specimen inlet 13, the specimen of the first measuring electrode 2 and the second measuring electrode 3 in the sample flow channel 12 by capillary phenomenon electrode portions 2a and 3a It will proceed to.

Disposable lysine sensor configured as described above, for example, it is used by attaching to the portable analyzer.
7 (a) is a schematic top view of a disposable lysine sensor and portable analyzer, FIG. 7 (b) is a schematic top view showing a state of mounting the disposable lysine sensor portable analyzer, Figure 8 is a portable analyzer the internal processing of the device is a block diagram schematically showing.
In the figure, symbol A is a disposable lysine sensor, reference numeral B denotes a portable analyzer, numeral 21 is a housing of the portable analyzer, reference numeral 22 denotes a display provided on the upper surface of the housing 21, reference numeral 23 an operation switch provided on the upper surface of the housing 21, reference numeral 24 denotes a sensor insertion port provided on one end of the housing 21, and, reference numeral 25 denotes each output terminal.
Figure 8 is a block diagram schematically showing the internal processing of the portable analyzer, numeral 26, 27 and 28, each terminal portion 2b of the disposable lysine sensor, 3b, a terminal connected to the 4b, reference numeral 29 a control unit for calculating the amount of lysine based on the input signal from the terminal 26, 27, 28, the reference numeral 30 is a storage device, reference numeral 22 denotes the display and reference numeral 25, denotes respectively the output terminal.

Hereinafter, we briefly describe how to use the disposable lysine sensor configured as described above.
As shown in FIG. 7 (b), the user inserting the disposable lysine sensor A which is prepared in the insertion port 24 of the portable analyzer B. Thus, the terminal portion 2b of the disposable lysine sensor A, 3b, 4b are connected to the terminals 26, 27 provided inside the portable analyzer B.
In this state, the user bled stabs his finger with a needle or the like, is brought into contact with the blood sample inlet 13, the blood is sucked into the sample flow path 12 by capillary action, the first measuring electrode 2 and second measuring electrode 3 of the electrode portions 2a and 3a, i.e., it moves to the reaction layer 6 and 7.
Then, in the first measuring electrode 2, contaminants lysine and sample solution in the sample solution is detected.
Moreover, it does not contain any lysine oxidase in the reaction layer 7, in the second measuring electrode 3, only contaminants in the sample liquid is detected.
The first measuring electrode 2 of the disposable lysine sensor A, a result of detection by the second measuring electrode 3 and counter electrode 4, each terminal portion 2b, 3b, through 4b, the portable analyzer B terminals 26 and 27, 28 is sent to the controller 29 from the control device 29, and the detection result of the reaction layer 6 (lysine and contaminants), by calculating the difference between the detection result (contaminants) in the reaction layer 7, the amount of lysine calculating a, with and stores the calculation result in the storage unit 30, if necessary, to display the calculation result on the display 22. The control device 29 is configured to be able to output to an external device from the output terminal 25 as necessary data stored in the storage unit 30.
Further, the control unit 29, based on the amount of the measured lysine, to determine malnutrition or not, may also be configured to output the determination result.

As described above, the ferricyanide ions to function as an electron acceptor lysine oxidase is confirmed, and also confirmed that the current value in the reaction system of lysine and the lysine oxidase is correlated with lysine amount because there, lysine oxidase in the first measurement best as in the embodiment described above, the surfactant and ferricyanide ions provided, a simple disposable sensor constructed to direct the sample to the first measurement electrode, the lysine concentration an effect that is possible to measure.

In the above described embodiment, on the second measuring electrode 3 of the electrode 3a, so to form a second reaction layer 7 containing a surfactant and ferricyanide ions between the first reaction layer 6 the same amount, measured in, matching conditions of the first measuring electrode 2 and the second measuring electrode 3, an effect that is obtained an accurate measurement result.

In the above embodiment, on the second measuring electrode 3 electrode 3a, it is provided with the second reaction layer 7 containing the first reaction layer 6 with the same amount of surfactant and an electron acceptor, the second reaction structure of layers 7 without being limited to this embodiment, for example, to align more conditions of the first reaction layer 6, a first reaction layer 6 the same amount of surfactant, electron acceptors and deactivated it may be formed by performing the coating and drying of the polymer solution of lysine oxidase is.

Furthermore, in the embodiment described above, the second measuring electrode 3 provided on the substrate, but to form a second reaction layer 7 on the second measuring electrode 3, the second measuring electrode 3 not an essential element , it may not be provided. For example, on the first measuring electrode 2, by holding the reagent capable of erasing the contaminants contained in the sample, it is possible to remove contaminants contained in the sample, the second measuring electrode 3 it is possible to obtain a measurement result which is not influenced by the contaminants without providing a. The large contaminant influence on the measurement of lysine, for example, L- ascorbic acid. In this case, ascorbic acid oxidase is provided as a reagent for removing the L- ascorbic acid on the first measuring electrode 2 obtain.

Implemented but, lysine oxidase used in the present invention as described above: In the above embodiment, as lysine oxidase, Trichoderma kink de subtilis -derived lysine oxidase (1.4.3.14 Sigma Aldrich Japan sold EC number) without being limited to the example, it is possible to use any enzyme as long as it is an enzyme that ferricyanide ions to function as an electron acceptor.
Specifically, may be a lysine oxidase derived from fungi other than Trichoderma kink de bacteria, also lysine oxidase except from molds, specifically, for example, it is extracted from immune system emanating from fish skin it may be a lysine oxidase and human origin of lysine oxidase.

A disposable lysine sensor 1 substrate 2 first measuring electrode 2a electrode 2b terminal 3 the second measuring electrode 3a electrode 3b terminal portions 4 counter 4a electrode 4b terminal portions 6 first reaction layer 7 second reaction layer 8 photoresist film 9 spacer 9a notched 10 air holes 11 cover 12 sample channel 13 sample inlet

B portable analyzer 21 housing 22 display 23 operation switch 24 sensor insertion port 25 the output terminal 26 terminal 27 terminal 28 terminal 29 controller 30 memory

Claims (9)

  1. Forming an electrode system having at least a measuring electrode and a counter electrode on an electrically insulating substrate,
    Enzyme, in a substance concentration change during the reaction of the electron acceptor and the sample was detected electrochemically the electrode system sensor for measuring the substrate concentration of the sample solution,
    Wherein the measuring electrode, lysine oxidase and disposable lysine sensor, characterized in that the lysine oxidase to form a reaction layer which holds the ferricyanide ions which react with lysine as a catalyst.
  2. Disposable lysine sensor according to claim 1, wherein the lysine oxidase, characterized in that it consists of an enzyme derived from fungus.
  3. Disposable lysine sensor according to claim 2, wherein the lysine oxidase, characterized in that it is an enzyme derived from bacteria of the genus Trichoderma.
  4. Disposable lysine sensor according to claim 3, wherein the lysine oxidase, characterized in that it is an enzyme derived from Trichoderma last place de bacteria.
  5. And wherein the second measuring electrode provided on an electrically insulating substrate, and the lysine oxidase said second measuring electrode to form a reaction layer containing no lysine oxidase holding the ferricyanide ions which react with lysine as a catalyst disposable lysine sensor according to any one of claims 1 to 4.
  6. A second measuring electrode provided on the electrically insulating substrate, that said second without measuring electrode to lysine oxidase active protein and lysine oxidase to form a reaction layer which holds the ferricyanide ions which react with lysine as a catalyst disposable lysine sensor according to any one of claims 1 to 4, wherein.
  7. Disposable lysine sensor according to claim 6, wherein the protein without the lysine oxidase activity is lysine oxidase was inactivated.
  8. Disposable lysine sensor according to any one of claims 1 to 4, characterized in that to hold the reagent to remove contaminants in the sample liquid to the reaction layer formed on the measuring electrode.
  9. The contaminants are L- ascorbic acid, disposable lysine sensor according to claim 8 in which the reagent for removing contaminating material, characterized in that ascorbic acid oxidase.
PCT/JP2012/058334 2011-03-29 2012-03-29 Disposable lysine sensor WO2012133633A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017009430A (en) * 2015-06-22 2017-01-12 株式会社村田製作所 Biosensor

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JP2003501627A (en) * 1999-06-02 2003-01-14 ノヴァ バイオメディカル コーポレイション Disposable sensor and manufacturing method
JP2007514931A (en) * 2003-10-31 2007-06-07 ライフスキャン・スコットランド・リミテッド Method of reducing the effects of direct interference currents and indirect interferent current in an electrochemical test strip
JP2008209274A (en) * 2007-02-27 2008-09-11 Citizen Holdings Co Ltd Electrochemical sensor
JP2009171874A (en) * 2008-01-23 2009-08-06 Citizen Holdings Co Ltd Method for measuring saccharified protein concentration, and biosensor

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JP2003501627A (en) * 1999-06-02 2003-01-14 ノヴァ バイオメディカル コーポレイション Disposable sensor and manufacturing method
JP2007514931A (en) * 2003-10-31 2007-06-07 ライフスキャン・スコットランド・リミテッド Method of reducing the effects of direct interference currents and indirect interferent current in an electrochemical test strip
JP2008209274A (en) * 2007-02-27 2008-09-11 Citizen Holdings Co Ltd Electrochemical sensor
JP2009171874A (en) * 2008-01-23 2009-08-06 Citizen Holdings Co Ltd Method for measuring saccharified protein concentration, and biosensor

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FRANCESCO RICCI ET AL.: 'Prussian Blue and enzyme bulk-modified screen-printed electrodes for hydrogen peroxide and glucose determination with improved storage and operational stability' ANALYTICA CHIMICA ACTA vol. 485, 26 May 2003, pages 111 - 120 *
HOLGER OLSCHEWSKI ET AL.: 'Screen-printed enzyme sensors for L-lysine determination' ENZYME AND MICROBIAL TECHNOLOGY vol. 26, no. 7, April 2000, pages 537 - 543 *

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017009430A (en) * 2015-06-22 2017-01-12 株式会社村田製作所 Biosensor

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