WO2012131723A1 - A herbal formulation to treat mastitis in animals - Google Patents
A herbal formulation to treat mastitis in animals Download PDFInfo
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- WO2012131723A1 WO2012131723A1 PCT/IN2012/000219 IN2012000219W WO2012131723A1 WO 2012131723 A1 WO2012131723 A1 WO 2012131723A1 IN 2012000219 W IN2012000219 W IN 2012000219W WO 2012131723 A1 WO2012131723 A1 WO 2012131723A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/27—Asclepiadaceae (Milkweed family), e.g. hoya
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Definitions
- the present invention relates to a herbal formulation to treat mastitis in animals. More particularly, the herbal formulation of present invention is prepared from Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis for the treatment of mastitis.
- Pergularia daemia belongs to family Asclepiadaceae. It is a perennial herb growing widely along the road sides of India and is also known by the names Utaran, Sagovani, Aakasan, Gadaria Ki bel and Jutak. It has been used in the folk medicine to treat liver disorders. It is astringent, acrid, emetic, expectorant and laxative.
- Cenchrus ciliaris is a perennial grass, about 50 cm tall, native to India. Cenchrus ciliaris, commonly known as buff el grass and belongs to the Family: Poaceae (alt. Gramineae). It is mainly used as a permanent pasture, but can be used for hay or silage. The dried shoots provide an excellent fuel for fire, from which the plant recovers rapidly by basal shoots. It is not suited to short-term pasture because it is too difficult to remove and binds nutrient.
- Abutilon indicum belongs to family Malvaceae. It is a herbaceous, tomentose plant, grows as a weed, and is found abundandy in wastelands from the seashore to 1,200 meters high in India and in the sub- Himalayan tracts. It is widely used for medicinal purpose like in deafness, ringing in the ears, earache, eye problems, cold, high fever, mumps, hives, pulmonary tuberculosis, cough, bronchitis, lack of PAT 121 urination, difficult/painful urination, diabetes, thirst, painful menses, carbuncle hemorrhoids, hernia, diarrhea, worms, infusion, poultice or paste for boils, ulcers.
- Coccinia grandis is a tropical vine also known as ivy gourd belongs to family Cucurbetaceae. This plant is grown for its edible young shoots and edible fruits. In traditional medicine, fruits have been used to treat leprosy and bronchitis. The plant pacifies vitiated kapha, pitta, constipation, burning sensation, leucorrhea, skin disease, fever, asthma, cough and jaundice.
- the synergistic formulation prepared from Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis for treatment of mastitis is not known in art.
- the present invention relates to a herbal formulation prepared from Pergularia daemia, Brachiaria eruciformis, Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis.
- the herbal composition of the present invention is used in the treatment of mastitis.
- the present invention provides a herbal formulation for the treatment of mastitis. More particularly, the present invention provides a herbal composition to treat mastitis comprising Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis.
- the herbal composition comprises combination of extracts of plants Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis, prepared by a process comprising the steps of :
- step (ii) sieving the plant material as obtained in step (i) followed by mixing to obtain a mixture of plant materials
- step (iii) grinding the mixture as obtained in step (ii) in the presence of water, added in an amount sufficient to obtain herbal composition; PAT 121
- step (iv) optionally mixing the herbal composition of step (iii) in water, boiling the mixture to obtain the herbal composition in the form of extract.
- the herbal composition comprises the plant material in a proportion of: 35 to 45 % of Pergularia daemia, 5 to 15 % of Brachiaria eruciformis, 10 to 15 % of Cenchrus ciliaris, 15 to 25 % of Abutilon indicum, and 15 to 25 % of Coccinia grandis.
- the plant material in step (ii) is mixed in a proportion of: 35 to 45 wt/wt% of Pergularia daemia, 5 to 15 wt/wt% of Brachiaria eruciformis., 10 to 15 wt/wt% Cenchrus ciliaris, 15 to 25 wt/wt% of Abutilon indicum and 15 to 25 wt/wt% of Cocinnia grandis.
- extracts of plants include both “paste” as well as “aqueous extract” of plant or plant parts.
- paste refers to the paste obtained by grinding the whole plant or plant parts selected from leaf, rhizome, seed, root, bark and fruit. Water can be used in the grinding process.
- Aqueous extract means extract of the plant or the plant parts in water.
- the plant material is selected from the group consisting of a whole plant, a rhizome, a leaf, a seed, a root, a bark, a flower or a fruit.
- plant material is leaves of Pergularia daemia, whole plant of Brachiaria eruciformis., whole plant of Cenchrus ciliaris, leaves of Abutilon indicum, and leaves of Coccinia grandis
- composition of the present invention may be administered as such or in admixture with a pharmaceutically accepted vehicle or an adjuvant
- the herbal composition can be formulated in the form of decoction, dried decoction, infusion, tincture, dried extract, capsule, tablet, syrup or the like.
- the formulation with a concentration of 400mg per ml is prepared from water extract of medicinal PAT 121 plant. Eight clinical cases were chosen for the study. In the study population it was found that 4 animals were in second lactation and two each in third & first stage of lactation. Among the test animal population one clinical case belongs to Buffalo species and remaining seven were cross bred animals. Except for two clinical cases, remaining six animals, mastitis was limited to single quarter. a. Collection of milk sample:
- Teats were washed thoroughly and dried with a singleuse paper towel. The first three streams of milk from each teat were discarded. The teat end and orifice was sanitized with cotton swabs soaked in 70% ethyl alcohol and approximately 5 ml for milk sample were collected from each quarter of cow in a sterile tube. b. Isolation of causative organism;
- the affected milk quarter were observed for 10 days starting from Day O (pretreatment period), Day I, Day II, Day III, Day VI and Day X.
- the diagnosis of the ailment was based on signs, clinical examination, result through pH paper and bacteriological culture. Most of the animals had shown clinical signs for 0-5 days before treatment.
- the prescribed duration of treatment ranged from 2 days to 4 days.
- the clinical condition had improved upon administering the medication indicating its therapeutic role in curing mastitis due to Pseudomonas aeruginosa, a common bacteria.
- the formulation had exhibited inhibitory effect of 9.5 ⁇ 0.31 Diameter in mm at 5 mg concentration over Staphylococcus aureus.
- the clinical condition affected with conforms were cured after administration of medication.
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
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- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
A herbal formulation prepared from Pergularia daemia, Brachiaria eniciformis., Cenchrus ciliaris, Abutilon
indicum and Cocinnia grandis for the treatment of mastitis.
Description
PAT 121
A HERBAL FORMULATION TO TREAT MASTITIS IN ANIMALS
FIELD OF INVENTION
The present invention relates to a herbal formulation to treat mastitis in animals. More particularly, the herbal formulation of present invention is prepared from Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis for the treatment of mastitis.
BACKGROUND OF THE INVENTION
Pergularia daemia belongs to family Asclepiadaceae. It is a perennial herb growing widely along the road sides of India and is also known by the names Utaran, Sagovani, Aakasan, Gadaria Ki bel and Jutak. It has been used in the folk medicine to treat liver disorders. It is astringent, acrid, emetic, expectorant and laxative.
Reference is made to the Journal of Ethnopharmacology, Volume 107, Issue 2, September 19, 2006, pp- 164-168, "Hepatoprotective effects of extracts from Pergularia daemia", by S;V.Sureshkumar and S.H.Mishra wherein it has been disclosed that the ethanolic extracts obtained from aerial parts of Pergularia daemia possess significant hepatoprotective activity . It is also used for cough and increases the levels of total protein. It lowers total cholesterol and cures digestive and menstrual disorders.
Cenchrus ciliaris is a perennial grass, about 50 cm tall, native to India. Cenchrus ciliaris, commonly known as buff el grass and belongs to the Family: Poaceae (alt. Gramineae). It is mainly used as a permanent pasture, but can be used for hay or silage. The dried shoots provide an excellent fuel for fire, from which the plant recovers rapidly by basal shoots. It is not suited to short-term pasture because it is too difficult to remove and binds nutrient.
Abutilon indicum belongs to family Malvaceae. It is a herbaceous, tomentose plant, grows as a weed, and is found abundandy in wastelands from the seashore to 1,200 meters high in India and in the sub- Himalayan tracts. It is widely used for medicinal purpose like in deafness, ringing in the ears, earache, eye problems, cold, high fever, mumps, hives, pulmonary tuberculosis, cough, bronchitis, lack of
PAT 121 urination, difficult/painful urination, diabetes, thirst, painful menses, carbuncle hemorrhoids, hernia, diarrhea, worms, infusion, poultice or paste for boils, ulcers.
Coccinia grandis is a tropical vine also known as ivy gourd belongs to family Cucurbetaceae. This plant is grown for its edible young shoots and edible fruits. In traditional medicine, fruits have been used to treat leprosy and bronchitis. The plant pacifies vitiated kapha, pitta, constipation, burning sensation, leucorrhea, skin disease, fever, asthma, cough and jaundice.
The synergistic formulation prepared from Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis for treatment of mastitis is not known in art.
SUMMARY OF THE INVENTION
The present invention relates to a herbal formulation prepared from Pergularia daemia, Brachiaria eruciformis, Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis. The herbal composition of the present invention is used in the treatment of mastitis.
DESCRIPTION OF THE INVENTION
The present invention provides a herbal formulation for the treatment of mastitis. More particularly, the present invention provides a herbal composition to treat mastitis comprising Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis.
The herbal composition comprises combination of extracts of plants Pergularia daemia, Brachiaria eruciformis., Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis, prepared by a process comprising the steps of :
(i) washing plant material obtained from the plants Pergularia daemia, Brachiaria eruciformis.,
Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis;
(ii) sieving the plant material as obtained in step (i) followed by mixing to obtain a mixture of plant materials;
(iii) grinding the mixture as obtained in step (ii) in the presence of water, added in an amount sufficient to obtain herbal composition;
PAT 121
(iv) optionally mixing the herbal composition of step (iii) in water, boiling the mixture to obtain the herbal composition in the form of extract.
The herbal composition comprises the plant material in a proportion of: 35 to 45 % of Pergularia daemia, 5 to 15 % of Brachiaria eruciformis, 10 to 15 % of Cenchrus ciliaris, 15 to 25 % of Abutilon indicum, and 15 to 25 % of Coccinia grandis.
In an embodiment, the plant material in step (ii) is mixed in a proportion of: 35 to 45 wt/wt% of Pergularia daemia, 5 to 15 wt/wt% of Brachiaria eruciformis., 10 to 15 wt/wt% Cenchrus ciliaris, 15 to 25 wt/wt% of Abutilon indicum and 15 to 25 wt/wt% of Cocinnia grandis.
The term "extracts" of plants include both "paste" as well as "aqueous extract" of plant or plant parts. The term paste refers to the paste obtained by grinding the whole plant or plant parts selected from leaf, rhizome, seed, root, bark and fruit. Water can be used in the grinding process. Aqueous extract means extract of the plant or the plant parts in water.
In another embodiment, the plant material is selected from the group consisting of a whole plant, a rhizome, a leaf, a seed, a root, a bark, a flower or a fruit. In another preferred embodiment, plant material is leaves of Pergularia daemia, whole plant of Brachiaria eruciformis., whole plant of Cenchrus ciliaris, leaves of Abutilon indicum, and leaves of Coccinia grandis
The composition of the present invention may be administered as such or in admixture with a pharmaceutically accepted vehicle or an adjuvant
The herbal composition can be formulated in the form of decoction, dried decoction, infusion, tincture, dried extract, capsule, tablet, syrup or the like.
Example :
The formulation with a concentration of 400mg per ml is prepared from water extract of medicinal
PAT 121 plant. Eight clinical cases were chosen for the study. In the study population it was found that 4 animals were in second lactation and two each in third & first stage of lactation. Among the test animal population one clinical case belongs to Buffalo species and remaining seven were cross bred animals. Except for two clinical cases, remaining six animals, mastitis was limited to single quarter. a. Collection of milk sample:
Teats were washed thoroughly and dried with a singleuse paper towel. The first three streams of milk from each teat were discarded. The teat end and orifice was sanitized with cotton swabs soaked in 70% ethyl alcohol and approximately 5 ml for milk sample were collected from each quarter of cow in a sterile tube. b. Isolation of causative organism;
Very little attention is paid to the usefulness of culturing bacteria and developing treatment protocol. Hence the milk samples were tested to isolate the causative organism. One field sample had shown bacterial culture indicating Pseudomonas aeruginosa as an etiological agent for mastitis. Staphylococcus sp., was also identified among the milk sample. c. Observation period:
The affected milk quarter were observed for 10 days starting from Day O (pretreatment period), Day I, Day II, Day III, Day VI and Day X. The diagnosis of the ailment was based on signs, clinical examination, result through pH paper and bacteriological culture. Most of the animals had shown clinical signs for 0-5 days before treatment. The prescribed duration of treatment ranged from 2 days to 4 days.
HI. Case history and clinical examination*:
IV. Ph sical examination of udder and milk; Da I Da II Da HI Da VI and Da X
V. Bacterial culture
Result:
The clinical condition had improved upon administering the medication indicating its therapeutic role in curing mastitis due to Pseudomonas aeruginosa, a common bacteria. The formulation had exhibited inhibitory effect of 9.5 ± 0.31 Diameter in mm at 5 mg concentration over Staphylococcus aureus. The clinical condition affected with conforms were cured after administration of medication.
ADVANTAGES OF THE INVENTION
Cost effective, overcomes antibacterial resistance
Claims
1. A herbal composition for treatment of mastitis, the said composition comprising combination of extracts of plants Pergularia daemia, Brachiaria eruciformis, Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis, prepared by a process comprising the steps of :
(i) washing plant material obtained from the plants Pergularia daemia, Brachiaria eruciformis, Cenchrus ciliaris, Abutilon indicum and Cocinnia grandis;
(ii) sieving the plant material as obtained in step (i) followed by mixing to obtain a mixture of plant materials;
(iii) grinding the mixture as obtained in step (ii) in the presence of water, added in an amount sufficient to obtain herbal composition;
(iv) optionally mixing the herbal composition of step (iii) in water, boiling the mixture to obtain the herbal composition in the form of extract.
2. The herbal composition as claimed in claim 1, wherein the plant material in step (i) is selected from the group consisting of a whole plant , a rhizome, a leaf, a seed, a root, a bark, a flower, a fruit or a combination thereof.
3. The herbal composition as claimed in claim 1, wherein the plant material in step (ii) is mixed in a proportion of: 35 to 45 wt/wt% of Pergularia daemia, 5 to 15 wt/wt% of Brachiaria eruciformis., 10 to 15 wt/wt% Cenchrus ciliaris, 15 to 25 wt/wt% of Abutilon indicum and 15 to 25 wt/wt% of Cocinnia grandis.
4. The composition as claimed in any of the preceding claims, wherein the plant material is a combination of leaves of Pergularia daemia, whole plant of Brachiaria eruciformis., whole plant of
Cenchrus ciliaris, leaves of Abutilon indicum, and leaves of Coccinia grandis
4. The composition as claimed in any of the preceding claims in admixture with a pharmaceutically acceptable vehicle or an adjuvant. PAT 121
5. The composition as claimed in any of the preceding claims, wherein the composition is formulated in a form selected from the group consisting of decoction, dried decoction, infusion, tincture, dried extract, capsule, tablet, syrup or the like thereof.
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Cited By (1)
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WO2014158671A1 (en) * | 2013-03-14 | 2014-10-02 | Avon Products, Inc | Abutilon indicum extracts and methods of use |
Citations (1)
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US20090317364A1 (en) * | 2006-07-10 | 2009-12-24 | Chandrakant Laxminarayan Rathi | Novel compositions for prevention and treatment of mastitis and metritis |
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US20090317364A1 (en) * | 2006-07-10 | 2009-12-24 | Chandrakant Laxminarayan Rathi | Novel compositions for prevention and treatment of mastitis and metritis |
Non-Patent Citations (8)
Title |
---|
DATABASE TKDL [online] 1976, "Thodathirku Ennei", XP002680940, Database accession no. GP02/986 * |
DEEPIKA THENMOZHI C ET AL: "Preliminary phytochemical screening and antibacterial activity of Pergularia daemia forsk", INTERNATIONAL JOURNAL OF PHARMA AND BIO SCIENCES, vol. 2, no. 1, 1 January 2011 (2011-01-01) - 1 March 2011 (2011-03-01), INTERNATIONAL JOURNAL OF PHARMA AND BIO SCIENCES IND, pages B-161 - B-166, XP009161571, ISSN: 0975-6299 * |
LIGHT M E ET AL: "Screening of Cenchrus ciliaris L. for biological activity.", SOUTH AFRICAN JOURNAL OF BOTANY, vol. 68, no. 3, September 2002 (2002-09-01), pages 411 - 413, XP009161515, ISSN: 0254-6299 * |
MANJULA M ET AL: "In vitro action of Coccinia grandis against bacterial organisms", ASIAN JOURNAL OF MICROBIOLOGY, BIOTECHNOLOGY AND ENVIRONMENTAL SCIENCES, vol. 11, no. 2, 2009, GLOBAL SCIENCE PUBLICATIONS IND, pages 317 - 320, XP009161519, ISSN: 0972-3005 * |
POONKOTHAI: "ANTIBACTERIAL ACTIVITY OF LEAF EXTRACT OF Abutilon indicum", ANCIENT SCIENCE OF LIFE VOL, 1 January 2006 (2006-01-01), pages 38 - 41, XP055033867, Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335236/pdf/ASL-26-39.pdf> [retrieved on 20120725] * |
S.V.SURESHKUMAR; S.H.MISHRA: "Hepatoprotective effects of extracts from Pergularia daemia", JOURNAL OF ETHNOPHARMACOLOGY, vol. 107, no. 2, 19 September 2006 (2006-09-19), pages 164 - 168 |
UDAYA PRAKASH N K ET AL: "A study on antibacterial activity of common weeds in Northern districts of Tamil Nadu, India", RESEARCH JOURNAL OF MEDICINAL PLANT, vol. 6, no. 4, 2012, ACADEMIC JOURNALS INC. USA, pages 341 - 345, XP055033870, ISSN: 1819-3455, DOI: 10.3923/RJMP.2012.341.345 * |
YUGI: "Yugi Munivar Vaithya Chinthamani", 1976, PALANI THANDAYUTHAPANI DEVASTHANAM, pages: 507 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014158671A1 (en) * | 2013-03-14 | 2014-10-02 | Avon Products, Inc | Abutilon indicum extracts and methods of use |
US9757327B2 (en) | 2013-03-14 | 2017-09-12 | Avon Products, Inc. | Abutilon indicum extracts and methods of use |
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