WO2012131715A2 - A herbal medication to cure jaundice - Google Patents
A herbal medication to cure jaundice Download PDFInfo
- Publication number
- WO2012131715A2 WO2012131715A2 PCT/IN2012/000210 IN2012000210W WO2012131715A2 WO 2012131715 A2 WO2012131715 A2 WO 2012131715A2 IN 2012000210 W IN2012000210 W IN 2012000210W WO 2012131715 A2 WO2012131715 A2 WO 2012131715A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- herbal
- plant
- jaundice
- plant material
- herbal composition
- Prior art date
Links
- 206010023126 Jaundice Diseases 0.000 title claims abstract description 22
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- 239000003814 drug Substances 0.000 title abstract description 11
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
Definitions
- the present invention relates to a herbal medication to cure jaundice. More particularly, the present invention relates to a herbal medication prepared from Prosopis cineraria for the treatment of jaundice.
- Jaundice is a condition in which a person's skin, the conjunctival membranes over the sclerae (whites of the eyes) and other mucous membranes are discolored yellow due to an increased level of bilirubin in the blood. Jaundice is often caused due to a problem with the liver, gallbladder, or pancreas. Infections, use of certain drugs, cancer, blood disorders, gallstones, birth defects and a number of other medical conditions can lead to jaundice.
- the herbal remedies known to treat Jaundice includes: Ghritkumari (Aloe Vera), Kakmachi (Solanum Nigrum), Jaundice berry and Bhumiamla.
- Prosopis cineraria is a plant commonly called shami or khejari. It belongs to family Fabaceae. It is found in Bengal, Haryana.ON Pradesh. Bengal. Madhya Pradesh and Bengal and also in Pakistan. It is the state tree of Bengal and the provincial tree of the Sindh provincial region of Pakistan. All the parts of this tree are useful so it is also called "kalp taru”. The medicinal benefits of khejri have been highlighted in the ancient Ayurveda literature.
- bioactive compounds found in khejri disclosed by Sharma, Garg and Paul (2010) are - flavonoides, diketones, phenolic content, free amino acids, Patulitrin, specigerine, prosogerine- A,B,C,D, lipids, sugars and vitamins.
- the flower of the plant mixed with sugar is used during pregnancy as safeguard against miscarriage.
- Water-soluble extract of the residue from methanol extract of the stem bark exhibits anti-inflammatory properties.
- the smoke of the leaves is good for eye troubles.
- the bark of the tree is dry, acrid, bitter with a sharp taste; cooling anthelmintic; tonic, cures leprosy, dysentery, bronchitis, asthma, leucoderma, piles and tremors of the muscles.
- the pod is considered astringent in Punjab.
- the bark is used as a remedy for rheumatism, in cough colds, asthma.
- the plant is recommended for the treatment of snakebite.
- the bark of the tree provides immediate relief to a person bitten by snake or scorpion.
- the medicines prepared from its bark are also used for treating diarrhea, dysentery, piles, worm infestations and other skin problems.
- the bark is also used to cure leprosy, bronchitis, asthma, tumor of muscles and to improve concentration.
- the gum of the tree is nutritive and good in taste and is used by pregnant woman at the time of delivery.
- Pushpangadan and Nayar (1994) disclose that the flowers of khejri are used for blood purification and curing skin diseases. Bark is used against summer boils, leprosy, dysentery, bronchitis, asthma, leucoderma and piles.
- the present invention relates to a herbal formulation prepared from Prosopis cineraria which is very effective in the treatment of jaundice.
- Another objective of present invention is to provide an effective and low cost method for treatment of jaundice.
- the present invention relates to a herbal medication prepared from Prosopis cineraria to cure jaundice.
- Fig 1 Shows DPPH Free radical scavenging activity
- Fig 2 Shows scavenging of hydrogen peroxide
- Fig 3 Shows ABTS radical cation decolorization assay
- Fig 4 Shows nitric oxide scavenging activity.
- the present invention provides a herbal medication to cure jaundice. More particularly, the present invention provides a herbal formulation prepared from Prosopis cineraria to cure jaundice.
- the herbal formulation of the present invention provides a formulation comprising an extract obtained from Prosopis cineraria, and is prepared by a process comprising the steps of : drying plant material derived from the plant Prosopis cineraria
- step (b) grinding and sieving the plant of step (a) to obtain the powdered plant material
- step (c) mixing the powdered plant material of step (b) in water;
- step (d) boiling the mixture obtained in step (c) to obtain herbal formulation.
- the plant material is collected and dried under shade at room temperature (25-45°C) for about 72 hours until the plant material is completely dry.
- the plant material is then ground into powdered form followed by sieving to obtain fine powder of the plant material.
- the plant material is selected from the group consisting of a whole plant, a rhizome, a leaf, a seed, a root, a bark, a flower or a fruit.
- the plant material is bark.
- the powdered plant material is then mixed with cold water and given to the patient suffering from jaundice.
- the powdered plant material is mixed with water in a ratio in the range of 200- to 400 gms in 1000 to 2000-mL water.
- the composition of the present invention may be administered as such or in admixture with a pharmaceutically acceptable vehicle, adjuvants, inactive excipients, diluents or lubricants.
- Non-limiting examples of pharmaceutically acceptable vehicle, adjuvants, inactive excipients, diluents or lubricants which can be used in the composition of the present invention include: cellulose, substituted cellulose, calcium carbonate, dicalcium phosphate, starches, lactose, modified food starches, dextrose, calcium sulfate, magnesium carbonate, magnesium stearate, stearic acid, glycerin, vegetable oils, polysorbates or lecithin.
- the herbal composition can be formulated in the form of decoction, dried decoction, infusion, tincture, dried extract, capsule, tablet, syrup or the like.
- the formulation of the present invention is prepared by mixing 1 teaspoon of bark of Prosopis cineraria in one cup of water and consumed thrice a day for 8-16 days.
- Total phenolic (TP) content of crude alcoholic extracts was determined by The Folin-Ciocalteu method [28]. To 1ml of methanolic plant extracts of various concentrations 5ml of 1:10 diluted FC reagent was added followed by 4ml of 1M Sodium carbonate solution. The absorbance of the colored reaction product was measured at 750 nm after 30min incubation in dark at room temperature. A calibration curve was created using different concentrations of standard Gallic acid. All readings were performed in triplicates and the level of Total Phenol in the extract was calculated from the standard calibration curve. Results were expressed in Gallic Acid Equivalents (mg GAE/g). b. DPPH Free radical scavenging activity:
- Hydrogen Peroxide has absorption maxima at 230nm and its reactivity is reduced by the free radical quenchers in the extract [30].
- a solution of hydrogen peroxide (20 mM) was prepared in phosphate buffered saline (20mM, pH 7.4). 1ml of methanolic extract of various concentrations was added to 2ml of hydrogen peroxide solution. Then finally the absorbance was measured at 230nm after 10 minutes at room temperature. All readings were performed in triplicates and the free radical scavenging activity was calculated from equation: [(A0-A)/A0] xlOO, where AO is the absorbance of Reagent blank and A is the absorbance of the test sample.
- a calibration curve was created using different concentrations of standard Ascorbic acid. All readings were performed in triplicates and the %Inhibition of the extract was calculated from the standard calibration curve. Results were compared with Standard Ascorbic acid. d. ABTS radical cation decolorization assay:
- ABTS radical cation 50ml of 2mM ABTS and 0.3mL of 17mM Potassium persulfate were mixed together and incubated in the dark for 12-16 h to develop Prussian blue colored ABTS-+ solution which has an absorption maxima at 734nm [31].
- 400pL of plant extracts of different concentrations was added to 320pL of ABTS-+ solution. The absorbance was measured at 734nm after 10 minutes at room temperature.
- Nitric oxide radical scavenging was estimated on the basis of Griess Illosvoy reaction using method followed by [32].
- Griess-Illosvoy reagent was modified by using naphthyl ethylene diamine dihydrochloride (0.1% w/v) instead of 1-napthylamine (5%). 6ml reaction mixture containing 2ml lOmM sodium nitroprusside, 1ml phosphate buffer saline and 1ml methanolic extracts of varying concentrations was incubated at 25°C for 150 min.
- Literature reveals that antioxidant activity of plant extract is mainly due to presence of phenolic compounds, which may exerts antioxidant effects as free radical scavengers, as hydrogen donating sources or as singlet oxygen quenchers and metal ion chelators [33].
- phenolic compounds which may exerts antioxidant effects as free radical scavengers, as hydrogen donating sources or as singlet oxygen quenchers and metal ion chelators [33].
- methanolic plant extract showed total phenolic content 0.05 mg/ml and is equivalent to 0.21 mg/ml gallic acid.
- DPPH is a purple color dye having absorption maxima of 517 nm and upon reaction with a hydrogen donor the purple color fades or disappears due to conversion of it to 2, 2-diphenyl-l-picryl hydrazine resulting in decrease in absorbance.
- Alcoholic extract exhibited free radical scavenging activity more than that of standard Ascorbic and this is clearly visible in fig. Standard ascorbic acid was found to have an IC50 value at 0.12mg/ml. Alcoholic extract has shown to have a good IC50 value at 0.014 mg/ml.
- Decolorization of the ABTS ' + radical cation similar to DPPH reflects the capacity of an antioxidant species to donate electrons or hydrogen atom to inactivate this radical species.
- the methanolic extracts exhibited greater ABTS ' + cation scavenging activity compared to Standard Ascorbic acid at 6pg/ml which is perceptible in the fig.
- the IC50 values of Ascorbic acid and plant extract were 5.8 pg/ml and 5 pg/ml respectively.
- Nitric oxide is implicated for inflammation, cancer and other pathological conditions. Hence, nitric oxide scavenging capacity of the extract may help to arrest the chain of reactions initiated by excess generation of nitric oxide that are detrimental to the human health [34]. Scavenging of nitric oxide radical is based on the generation of nitric oxide. Sodium nitroprusside in buffered saline, which reacts with oxygen to produce nitrite ions, measured by using Griess reagent. Perceptible in the fig plant extract decreased the amount of nitrite ions comparatively more than that of Ascorbic acid. The IC50 values of Ascorbic acid and plant extract were 1.1 mg/ml and 0.82mg/ml.
- the herbal formulation prepared according to present invention is effective for curing jaundice.
- the herbal composition used in present invention provides relief without any side effect.
- the present invention provides an effective and low cost method for curing jaundice .
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Abstract
The present invention relates to a herbal medication to cure jaundice. More particularly, the present invention relates to a herbal medication prepared from Prosopis cineraria for the treatment of jaundice. The herbal formulation prepared according to present invention is effective for curing jaundice. The herbal composition used in present invention provides relief without any side effect. The present invention provides an effective and low cost method for curing jaundice.
Description
"A HERBAL MEDICATION TO CURE JAUNDICE
FIELD OF INVENTION
The present invention relates to a herbal medication to cure jaundice. More particularly, the present invention relates to a herbal medication prepared from Prosopis cineraria for the treatment of jaundice.
BACKGROUND OF THE INVENTION
Jaundice is a condition in which a person's skin, the conjunctival membranes over the sclerae (whites of the eyes) and other mucous membranes are discolored yellow due to an increased level of bilirubin in the blood. Jaundice is often caused due to a problem with the liver, gallbladder, or pancreas. Infections, use of certain drugs, cancer, blood disorders, gallstones, birth defects and a number of other medical conditions can lead to jaundice. The herbal remedies known to treat Jaundice includes: Ghritkumari (Aloe Vera), Kakmachi (Solanum Nigrum), Jaundice berry and Bhumiamla. Prosopis cineraria is a plant commonly called shami or khejari. It belongs to family Fabaceae. It is found in Rajasthan, Haryana. Uttar Pradesh. Punjab. Madhya Pradesh and Gujarat and also in Pakistan. It is the state tree of Rajasthan and the provincial tree of the Sindh provincial region of Pakistan. All the parts of this tree are useful so it is also called "kalp taru".The medicinal benefits of khejri have been highlighted in the ancient Ayurveda literature.
A variety of chemicals have been discovered from various parts of Prosopis cineraria. The stem bark contains vitamin K, n-octacosyl acetate, the long chain aliphatic acid. Presence of glucose, rhamnose, sucrose and starch is also reported. A cytotoxic principle, patulibin, has been isolated from flowers. It is also found to contain Piperidin alkaloids, Juliflorinine and N-methyjulifloridine, Julifloricene, and Julifloricene. These compounds show significant anti-fungal activities against dermatophytes. The fruits yield a Flavoneglycoside Patulitrin which exhibit cytotoxic activity. Numerous bioactive compounds found in khejri disclosed by Sharma, Garg and Paul (2010) are - flavonoides, diketones,
phenolic content, free amino acids, Patulitrin, specigerine, prosogerine- A,B,C,D, lipids, sugars and vitamins.
The flower of the plant mixed with sugar is used during pregnancy as safeguard against miscarriage. Water-soluble extract of the residue from methanol extract of the stem bark exhibits anti-inflammatory properties. The smoke of the leaves is good for eye troubles. The bark of the tree is dry, acrid, bitter with a sharp taste; cooling anthelmintic; tonic, cures leprosy, dysentery, bronchitis, asthma, leucoderma, piles and tremors of the muscles. The pod is considered astringent in Punjab. The bark is used as a remedy for rheumatism, in cough colds, asthma. The plant is recommended for the treatment of snakebite. The bark of the tree provides immediate relief to a person bitten by snake or scorpion. Its leaves and fruits are used in preparing medicines for curing nervous disorders. The medicines prepared from its bark are also used for treating diarrhea, dysentery, piles, worm infestations and other skin problems. The bark is also used to cure leprosy, bronchitis, asthma, tumor of muscles and to improve concentration. The gum of the tree is nutritive and good in taste and is used by pregnant woman at the time of delivery. Pushpangadan and Nayar (1994) disclose that the flowers of khejri are used for blood purification and curing skin diseases. Bark is used against summer boils, leprosy, dysentery, bronchitis, asthma, leucoderma and piles.
The present invention relates to a herbal formulation prepared from Prosopis cineraria which is very effective in the treatment of jaundice.
OBJECTIVE OF THE INVENTION
It is an objective of the present invention to provide a herbal formulation for curing jaundice.
Another objective of present invention is to provide an effective and low cost method for treatment of jaundice.
SUMMARY OF THE INVENTION
The present invention relates to a herbal medication prepared from Prosopis cineraria to cure jaundice.
BRIEF DESCRIPTION OF DRAWINGS:
Graph 1: Shows standard Graph of Gallic Acid: Concentration VS Absorbance
Fig 1: Shows DPPH Free radical scavenging activity
Fig 2: Shows scavenging of hydrogen peroxide
Fig 3: Shows ABTS radical cation decolorization assay
Fig 4: Shows nitric oxide scavenging activity.
DESCRIPTION OF THE INVENTION
The present invention provides a herbal medication to cure jaundice. More particularly, the present invention provides a herbal formulation prepared from Prosopis cineraria to cure jaundice.
The herbal formulation of the present invention provides a formulation comprising an extract obtained from Prosopis cineraria, and is prepared by a process comprising the steps of : drying plant material derived from the plant Prosopis cineraria
(b) grinding and sieving the plant of step (a) to obtain the powdered plant material;
(c) mixing the powdered plant material of step (b) in water;
(d) boiling the mixture obtained in step (c) to obtain herbal formulation. The plant material is collected and dried under shade at room temperature (25-45°C) for about 72 hours until the plant material is completely dry. The plant material is then ground into powdered form followed by sieving to obtain fine powder of the plant material.
In an embodiment, the plant material is selected from the group consisting of a whole plant, a rhizome, a leaf, a seed, a root, a bark, a flower or a fruit.
In another preferred embodiment, the plant material is bark.
The powdered plant material is then mixed with cold water and given to the patient suffering from jaundice.
In still another embodiment, the powdered plant material is mixed with water in a ratio in the range of 200- to 400 gms in 1000 to 2000-mL water. The composition of the present invention may be administered as such or in admixture with a pharmaceutically acceptable vehicle, adjuvants, inactive excipients, diluents or lubricants.
Non-limiting examples of pharmaceutically acceptable vehicle, adjuvants, inactive excipients, diluents or lubricants which can be used in the composition of the present invention include: cellulose, substituted cellulose, calcium carbonate, dicalcium phosphate, starches, lactose, modified food starches, dextrose, calcium sulfate, magnesium carbonate, magnesium stearate, stearic acid, glycerin, vegetable oils, polysorbates or lecithin.
The herbal composition can be formulated in the form of decoction, dried decoction, infusion, tincture, dried extract, capsule, tablet, syrup or the like.
The herbal formulation in a dosage of 200-500 mg per 200-250mL water is given to the patient thrice a day for 8-16 days. · EXAMPLE:
The formulation of the present invention is prepared by mixing 1 teaspoon of bark of Prosopis cineraria in one cup of water and consumed thrice a day for 8-16 days.
About 250g of the powdered material was subjected to soxhlation, it was first defatted with petroleum ether (Hi-Media, Bangalore) and then exhaustively extracted with methanol (Hi-Media, Bangalore) for 48 hrs. The solvent was distilled off at low temperature under reduced pressure using rotory flash evaporator (Buchi, Flawil, Switzerland.). The yield was 39.8 % w/w. The extract was subjected to preliminary phytochemical tests.
Evaluation of Antioxidant Activity:
a. Determination of total phenol by the Folin-Ciocalteu assay:
Total phenolic (TP) content of crude alcoholic extracts was determined by The Folin-Ciocalteu method [28]. To 1ml of methanolic plant extracts of various concentrations 5ml of 1:10 diluted FC reagent was added followed by 4ml of 1M Sodium carbonate solution. The absorbance of the colored reaction product was measured at 750 nm after 30min incubation in dark at room temperature. A calibration curve was created using different concentrations of standard Gallic acid. All readings were performed in triplicates and the level of Total Phenol in the extract was calculated from the standard calibration curve. Results were expressed in Gallic Acid Equivalents (mg GAE/g). b. DPPH Free radical scavenging activity:
The free radical scavenging activity of extracts was studied by its ability to reduce the DPPH Assay [29]. Briefly, ΙΟΟμΜ solution of DPPH in methanol was prepared and 2 ml of this solution was added to ΙΟΟμΙ each of alcoholic extract of different concentrations. After 10 minutes of incubation in dark, the absorbance was measured at 517 nm. All readings were performed in triplicates and the free radical scavenging activity was calculated from equation: [(A0-A)/A0] xlOO, where AO is the absorbance of Reagent blank and A is the absorbance of the test sample. The percentage inhibition of plant extract, Ascorbic acid standard was plotted in X-axis against respective concentrations used in Y-axis and their IC50 value was calculated by extrapolating the graph. c. Scavenging of hydrogen peroxide:
Hydrogen Peroxide has absorption maxima at 230nm and its reactivity is reduced by the free radical quenchers in the extract [30]. A solution of hydrogen peroxide (20 mM) was prepared in phosphate buffered saline (20mM, pH 7.4). 1ml of methanolic extract of various concentrations was added to 2ml of hydrogen peroxide solution. Then finally the absorbance was measured at 230nm after 10 minutes at room temperature. All readings were performed in triplicates and the free radical scavenging activity was calculated from equation: [(A0-A)/A0] xlOO, where AO is the absorbance of Reagent blank and A is the absorbance of the test sample. A calibration curve was created using different concentrations of standard Ascorbic acid. All readings were performed in triplicates and the %Inhibition of the extract was calculated from the standard calibration curve. Results were compared with Standard Ascorbic
acid. d. ABTS radical cation decolorization assay:
To generate ABTS radical cation, 50ml of 2mM ABTS and 0.3mL of 17mM Potassium persulfate were mixed together and incubated in the dark for 12-16 h to develop Prussian blue colored ABTS-+ solution which has an absorption maxima at 734nm [31]. To determine scavenging activity of methanolic extracts, 400pL of plant extracts of different concentrations was added to 320pL of ABTS-+ solution. The absorbance was measured at 734nm after 10 minutes at room temperature. All readings were performed in triplicates and the free radical scavenging activity was calculated from equation: [(A0- A)/A0] xlOO, where AO is the absorbance of Reagent blank and A is the absorbance of the test sample. The percentage inhibition of plant extract, Ascorbic acid standard was plotted against respective concentrations used and their IC50 value was calculated by extrapolating the graph. e. Nitric oxide scavenging activity:
Nitric oxide radical scavenging was estimated on the basis of Griess Illosvoy reaction using method followed by [32]. In this investigation, Griess-Illosvoy reagent was modified by using naphthyl ethylene diamine dihydrochloride (0.1% w/v) instead of 1-napthylamine (5%). 6ml reaction mixture containing 2ml lOmM sodium nitroprusside, 1ml phosphate buffer saline and 1ml methanolic extracts of varying concentrations was incubated at 25°C for 150 min. After incubation, 1ml of the reaction mixture mixed with 1ml of sulfanilic acid reagent (0.33% in 20% glacial acetic acid) and allowed to stand for 5 min for completing diazotization. Then, 1 ml of naphthyl ethylene diamine dihydrochloride was added, mixed and allowed to stand for 30 min at 25°C. A pink colored chromophore formed in diffused light. The absorbance of these solutions was measured at 540 nm against the corresponding blank solutions. All readings were performed in triplicates and the free radical scavenging activity was calculated from equation: [(A0-A)/A0] xlOO, where AO is the absorbance of Reagent blank and A is the absorbance of the test sample. The percentage inhibition of plant extract, Ascorbic acid standard was plotted against respective concentrations used and their IC50 value was calculated by extrapolating the graph. Table: Showing the Assays performed with IC50 values of Plant Extract and the Standard Ascorbic
acid:-
Result: a. Estimation of total phenolic content
Literature reveals that antioxidant activity of plant extract is mainly due to presence of phenolic compounds, which may exerts antioxidant effects as free radical scavengers, as hydrogen donating sources or as singlet oxygen quenchers and metal ion chelators [33]. In our present investigation, it was found that methanolic plant extract showed total phenolic content 0.05 mg/ml and is equivalent to 0.21 mg/ml gallic acid.
b. DPPH Free radical scavenging activity
DPPH is a purple color dye having absorption maxima of 517 nm and upon reaction with a hydrogen donor the purple color fades or disappears due to conversion of it to 2, 2-diphenyl-l-picryl hydrazine resulting in decrease in absorbance. Alcoholic extract exhibited free radical scavenging activity more than that of standard Ascorbic and this is clearly visible in fig. Standard ascorbic acid was found to have an IC50 value at 0.12mg/ml. Alcoholic extract has shown to have a good IC50 value at 0.014 mg/ml.
c. Scavenging of hydrogen peroxide
Scavenging activity of H2O2 by plant extract may be attributed to their phenolics, which can donate electrons to H2O2, thus neutralizing it to water. The H2O2 scavenging activity was detected and compared with Ascorbic acid. Fig shows the % inhibition values of Ascorbic acid and methanolic plant extract respectively. The absorbance of the chromophore is measured at 230 nm in the presence of the fractions. The extract capable of scavenging H2O2 in a concentration dependent manner and was higher than that of standard. The Ic50 values of Ascorbic acid were found to be 0.4 mg/ml, 0.052 mg/ml.
d. ABTS radical cation decolorization assay:
Decolorization of the ABTS'+ radical cation similar to DPPH reflects the capacity of an antioxidant species to donate electrons or hydrogen atom to inactivate this radical species. The methanolic extracts exhibited greater ABTS'+ cation scavenging activity compared to Standard Ascorbic acid at 6pg/ml which is perceptible in the fig. The IC50 values of Ascorbic acid and plant extract were 5.8 pg/ml and 5 pg/ml respectively. e. Nitric oxide scavenging activity:
Nitric oxide is implicated for inflammation, cancer and other pathological conditions. Hence, nitric oxide scavenging capacity of the extract may help to arrest the chain of reactions initiated by excess generation of nitric oxide that are detrimental to the human health [34]. Scavenging of nitric oxide radical is based on the generation of nitric oxide. Sodium nitroprusside in buffered saline, which reacts with oxygen to produce nitrite ions, measured by using Griess reagent. Perceptible in the fig plant extract decreased the amount of nitrite ions comparatively more than that of Ascorbic acid. The IC50 values of Ascorbic acid and plant extract were 1.1 mg/ml and 0.82mg/ml.
ADVANTAGES OF THE INVENTION
The herbal formulation prepared according to present invention is effective for curing jaundice. The herbal composition used in present invention provides relief without any side effect. The present invention provides an effective and low cost method for curing jaundice .
Claims
1. A herbal formulation to cure jaundice, comprising extract obtained from the plant Prosopis cineraria, prepared by a process comprising the steps of :
(a) drying plant material derived from the plant Prosopis cineraria;
(b) grinding and sieving the plant of step (a) to obtain the powdered plant material;
(c) mixing the powdered plant material of step (b) in water;
(d) boiling the mixture obtained in step (c) to obtain herbal formulation.
2. The herbal composition as claimed in claim 1, wherein the plant material in step (a) is selected from the group consisting of a whole plant , a rhizome, a leaf, a seed, a root, a bark, a flower or a fruit.
3. The herbal composition as claimed in claim 1, wherein the plant material in step (a) is bark.
4. The herbal composition as claimed in step (c) of claim 1, wherein the powdered plant material is mixed with water in a ratio in the range of 200- to 400 gms in 1000 to 2000-mL water.
5. The herbal composition as claimed in any of the preceding claims, wherein the herbal composition is formulated in a form selected from the group consisting of decoction, dried decoction, infusion, tincture, dried extract, capsule, tablet, syrup or the like thereof.
6. The herbal composition as claimed in any of the preceding claims, wherein the herbal composition is in admixture with a pharmaceutically accepted vehicle or an adjuvant.
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