WO2012131646A2 - Herbal compositions for increase and improvement of milk yield in cattle - Google Patents
Herbal compositions for increase and improvement of milk yield in cattle Download PDFInfo
- Publication number
- WO2012131646A2 WO2012131646A2 PCT/IB2012/051571 IB2012051571W WO2012131646A2 WO 2012131646 A2 WO2012131646 A2 WO 2012131646A2 IB 2012051571 W IB2012051571 W IB 2012051571W WO 2012131646 A2 WO2012131646 A2 WO 2012131646A2
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- WIPO (PCT)
- Prior art keywords
- acacia
- leucophloea
- herbal compositions
- acacia leucophloea
- herbal
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
Definitions
- the synthetic medicines or compositions, and associated enhancement methods can be effective in increasing milk yield, but they pose the threat of side effects and chemical toxicity which can adversely affect a subject's overall health, and mostly involve high cost and effort in manufacturing.
- the object of the invention is to provide novel herbal compositions which may be cost effective and easily available for increase and improvement of milk yield in cattle, and which have less or no side effects to the health of a subject.
- the present invention in a preferred embodiment provides herbal compositions for increase and improvement of milk yield in a subject, the herbal composition comprising of at least a part of Acacia leucophloea, to which parts of one or more additional complimentary herb may be optionally added, wherein the additional complimentary herb is also a herb for increase and improvement of milk yield in a subject and is selected from but not limited Chenopodium ambrosioides seeds, Desmodium gangeticum leaves, Nigella sativa leaves, Launaea mucronata leaves, rice water, genera Centella, Withania, Pueraria, Asparagus, Chlorophytum, Boerhaavia, Sida, Saraca, Elettaria cardamomum, Piper longum, Myristica fragrans fruit, or any combination thereof.
- the invention also provides for methods of preparation of the herbal compositions.
- the present invention in a preferred embodiment provides herbal compositions for increase and improvement of milk yield in cattle, comprising of at least a part of Acacia leucophloea, to which parts of one or more additional complimentary herb may be optionally added, wherein the additional complimentary herb is also a herb for increase and improvement of milk yield in cattle and is selected from but not limited to Chenopodium ambrosioides seeds, Desmodium gangeticum leaves, Nigella sativa leaves, Launaea mucronata leaves, rice water, genera Centella, Withania, Pueraria, Asparagus, Chlorophytum, Boerhaavia, Sida, Saraca, Elettaria cardamomum, Piper longum, Myristica fragrans fruit, or any combination thereof.
- Parts of Acacia leucophloea for the purpose of this invention includes the whole Acacia leucophloea, extract of Acacia leucophloea, pulverized form of Acacia leucophloea, crushed form of Acacia leucophloea, pieces cut or severed from Acacia leucophloea, chemical constituents of Acacia leucophloea along with their derivatives, or any combination thereof.
- the parts of Acacia leucophloea are severed by different methods such as but not limited to cutting, slicing, pounding, grinding, mashing or mincing.
- composition' for the purpose of this invention means a substance or mixture comprising of at least one natural component having medicinal property, wherein the natural component shall include a component extracted or derived from a natural source such as a herb or a shrub or a tree or a plant or parts thereof.
- the term subject for the purpose of the invention means one or more of a human or any other animal which is capable of producing milk.
- the herbs used for the compositions in accordance with the present invention shall include other herbs of their respective species or family or genus which display similar medicinal properties.
- a solvent may be used for preparing the herbal compositions of the present invention, and the solvent used may be any suitable solvent such as but not limited to one or more of methanol, ethanol, n-propanol, isopropanol, hexane, heptane, petroleum ether, cyclohexane, diethyl ether, dusopropyl ether, ethyl acetate, methyl acetate, ethyl formate, methyl formate, isobutyl acetate, n-butyl acetate, methylene chloride, ethylene chloride, chloroform, carbon tetrachloride, acetone, ethyl methyl ketone, diisobutyl ketone, methyl isobutyl ketone, 1,4- dioxane, toluene, ammonia solution, glacial acetic acid, ammonium hydroxide, sodium hydroxide, calcium hydrox
- a suitable excipient is added to the herbal compositions of the present invention to prepare a consumable formulation for effective therapeutic delivery.
- the herbal compositions of the present invention may be formulated for oral administration by compounding the active ingredients with the usual nontoxic pharmaceutically acceptable excipients for tablets, pellets, granules, capsules, solutions, emulsions, suspensions, and any other form suitable for use.
- Formulations in accordance with the present invention may include one or more of talc, water, glucose, lactose, gum acacia, gelatin, mannitol, starch paste, magnesium trisilicate, corn starch, keratin, colloidal silica, potato starch, urea, and other excipients suitable for use in manufacturing preparations, in solid, semisolid or liquid form, or any combination thereof.
- auxiliary, stabilizing, thickening, flavoring, fragrance and coloring agents may be used.
- the herbal compositions can be administered in a solid form such as but not limited to tablets, capsules, pills, pellets, granules, boluses or powder.
- a solid form such as but not limited to tablets, capsules, pills, pellets, granules, boluses or powder.
- the herbal compositions may be mixed with a pharmaceutical excipient such as but not limited to corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums, along with other pharmaceutical diluents.
- the solid compositions may be further subdivided into unit dosage forms containing an effective amount of compositions of the present invention.
- the herbal compositions can be administered in a liquid form such as but not limited to aqueous solution, suitably flavored syrups, aqueous suspensions, oil suspensions, tinctures, tisanes, macerates and flavored emulsions with edible oils as well as elixirs or similar pharmaceutical carriers.
- the liquid form may include suitable dispersing or suspending agents for aqueous suspensions such as but not limited to tragacanth, acacia, alginate, dextran, sodium carboxymethyl cellulose, methylcellulose, polyvinylpyrrolidone or gelatin.
- Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for reconstitution with water or other suitable vehicles before use.
- a process for preparing herbal compositions for increase and improvement of milk yield comprise the steps of,
- a process for preparing an herbal composition for increase and improvement of milk yield comprise the steps of,
- a process for preparing an herbal composition for increase and improvement of milk yield comprise the steps of,
- a process for preparing an herbal composition for increase and improvement of milk yield comprises the steps of,
- the herbal compositions may be delivered as a semi-solid form or gelatinous solid, such as but not limited to paste, jelly, gel or chutney.
- the herbal compositions may be delivered as a gaseous form or in form of aerosols which includes but not limited to spray, fumes, inhalers or mist.
- the herbal compositions may also be formulated for parenteral administration by injection, which includes but is not limited to, using conventional catheterization techniques or infusion.
- the herbal compositions may also be formulated for topical administration, in the form of solution, cream, gel, ointment, powder, paste, gum or lozenge, using techniques known to those skilled in the formulation field.
- topical administration includes delivery of the compositions to mucosal tissue of the mouth, nose and throat by, for example, spray or mist application.
- the herbal compositions as an active ingredient may be used alone or in combination with other therapeutic agents, for increase and improvement of milk yield in cattle.
- the herbal compositions of the present invention may be in the form of a decoction or may be prepared using a decoction process.
- the parts of Acacia leucophloea can be given chemical treatment or made to react with other chemical reagents to form mixtures or herbal compositions which are used for increase and improvement of milk yield in cattle.
- the desirable dose of the compositions may vary depending on the condition and the weight of the subject, severity, drug form, route and period of administration.
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention in a preferred embodiment provides herbal compositions for increase and improvement of milk yield in cattle. The present invention also provides for the methods of preparation and various forms of administration of the said compositions. The said compositions comprise of at least a part of Acacia leucophloea.
Description
HERBAL COMPOSITIONS FOR INCREASE AND IMPROVEMENT OF MILK
YIELD IN CATTLE
BACKGROUND:
Obtaining increased yield of milk, more specifically from cattle, has been an important activity from the societal and commercial perspective.
Methods for increase and improvement of milk yield in cattle have been practiced over the years, including administering food supplements, breeding programs, and providing doses of synthetic drugs or hormones.
The synthetic medicines or compositions, and associated enhancement methods can be effective in increasing milk yield, but they pose the threat of side effects and chemical toxicity which can adversely affect a subject's overall health, and mostly involve high cost and effort in manufacturing.
Considering the significance of increasing milk yield of cattle, there is a continuous requirement for medicines or compositions for milk yield enhancement, which would be economical, easily available and may have lower side effects and lower toxicities affecting a subject's body, which can be addressed by herbal compositions of the present invention.
OBJECT OF THE INVENTION:
The object of the invention is to provide novel herbal compositions which may be cost effective and easily available for increase and improvement of milk yield in cattle, and which have less or no side effects to the health of a subject.
SUMMARY OF THE INVENTION:
The present invention in a preferred embodiment provides herbal compositions for increase and improvement of milk yield in a subject, the herbal composition comprising of at least a part of Acacia leucophloea, to which parts of one or more additional complimentary herb may be optionally added, wherein the additional complimentary herb is also a herb for increase and improvement of milk yield in a subject and is selected from but not limited
Chenopodium ambrosioides seeds, Desmodium gangeticum leaves, Nigella sativa leaves, Launaea mucronata leaves, rice water, genera Centella, Withania, Pueraria, Asparagus, Chlorophytum, Boerhaavia, Sida, Saraca, Elettaria cardamomum, Piper longum, Myristica fragrans fruit, or any combination thereof. The invention also provides for methods of preparation of the herbal compositions.
DETAILED DESCRITION OF THE INVENTION:
The present invention in a preferred embodiment provides herbal compositions for increase and improvement of milk yield in cattle, comprising of at least a part of Acacia leucophloea, to which parts of one or more additional complimentary herb may be optionally added, wherein the additional complimentary herb is also a herb for increase and improvement of milk yield in cattle and is selected from but not limited to Chenopodium ambrosioides seeds, Desmodium gangeticum leaves, Nigella sativa leaves, Launaea mucronata leaves, rice water, genera Centella, Withania, Pueraria, Asparagus, Chlorophytum, Boerhaavia, Sida, Saraca, Elettaria cardamomum, Piper longum, Myristica fragrans fruit, or any combination thereof.
Parts of Acacia leucophloea for the purpose of this invention includes the whole Acacia leucophloea, extract of Acacia leucophloea, pulverized form of Acacia leucophloea, crushed form of Acacia leucophloea, pieces cut or severed from Acacia leucophloea, chemical constituents of Acacia leucophloea along with their derivatives, or any combination thereof.
In an embodiment of the invention, the parts of Acacia leucophloea are severed by different methods such as but not limited to cutting, slicing, pounding, grinding, mashing or mincing.
The term 'herbal composition' for the purpose of this invention means a substance or mixture comprising of at least one natural component having medicinal property, wherein the natural component shall include a component extracted or derived from a natural source such as a herb or a shrub or a tree or a plant or parts thereof.
The term subject for the purpose of the invention means one or more of a human or any other animal which is capable of producing milk.
The herbs used for the compositions in accordance with the present invention, shall include other herbs of their respective species or family or genus which display similar medicinal properties.
In an embodiment of the invention, a solvent may be used for preparing the herbal compositions of the present invention, and the solvent used may be any suitable solvent such as but not limited to one or more of methanol, ethanol, n-propanol, isopropanol, hexane, heptane, petroleum ether, cyclohexane, diethyl ether, dusopropyl ether, ethyl acetate, methyl acetate, ethyl formate, methyl formate, isobutyl acetate, n-butyl acetate, methylene chloride, ethylene chloride, chloroform, carbon tetrachloride, acetone, ethyl methyl ketone, diisobutyl ketone, methyl isobutyl ketone, 1,4- dioxane, toluene, ammonia solution, glacial acetic acid, ammonium hydroxide, sodium hydroxide, calcium hydroxide, calcium carbonate, potassium hydroxide, potassium carbonate, water and other solvents known by those of skilled in the art.
In an embodiment of the invention, a suitable excipient is added to the herbal compositions of the present invention to prepare a consumable formulation for effective therapeutic delivery.
In an embodiment of the invention, the herbal compositions of the present invention may be formulated for oral administration by compounding the active ingredients with the usual nontoxic pharmaceutically acceptable excipients for tablets, pellets, granules, capsules, solutions, emulsions, suspensions, and any other form suitable for use. Formulations in accordance with the present invention may include one or more of talc, water, glucose, lactose, gum acacia, gelatin, mannitol, starch paste, magnesium trisilicate, corn starch, keratin, colloidal silica, potato starch, urea, and other excipients suitable for use in manufacturing preparations, in solid, semisolid or liquid form, or any combination thereof. Additionally, auxiliary, stabilizing, thickening, flavoring, fragrance and coloring agents may be used.
In an embodiment of the invention, the herbal compositions can be administered in a solid form such as but not limited to tablets, capsules, pills, pellets, granules, boluses or powder. For preparing the solid form, the herbal compositions may be mixed with a pharmaceutical excipient such as but not limited to corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums, along with other pharmaceutical diluents.
The solid compositions may be further subdivided into unit dosage forms containing an effective amount of compositions of the present invention.
In an embodiment of the invention, the herbal compositions can be administered in a liquid form such as but not limited to aqueous solution, suitably flavored syrups, aqueous suspensions, oil suspensions, tinctures, tisanes, macerates and flavored emulsions with edible oils as well as elixirs or similar pharmaceutical carriers. The liquid form may include suitable dispersing or suspending agents for aqueous suspensions such as but not limited to tragacanth, acacia, alginate, dextran, sodium carboxymethyl cellulose, methylcellulose, polyvinylpyrrolidone or gelatin. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for reconstitution with water or other suitable vehicles before use.
In an embodiment of the invention, a process for preparing herbal compositions for increase and improvement of milk yield, comprise the steps of,
a) washing a part of Acacia leucophloea;
b) grinding the part of Acacia leucophloea; and
c) preparing pharmaceutically acceptable formulation thereof.
In an embodiment of the invention, a process for preparing an herbal composition for increase and improvement of milk yield, comprise the steps of,
a) washing a part of Acacia leucophloea;
b) grinding the part of Acacia leucophloea;
c) mixing the part of Acacia leucophloea and the part of the additional herb; and d) preparing pharmaceutically acceptable formulation thereof.
In an embodiment of the invention, a process for preparing an herbal composition for increase and improvement of milk yield, comprise the steps of,
a) washing a part of fruit of Acacia leucophloea;
b) grinding the part of fruit of Acacia leucophloea;
c) mixing of the single fruit of Acacia leucophloea and rice water, and d) preparing pharmaceutically acceptable formulation thereof.
In an embodiment of the invention, a process for preparing an herbal composition for increase and improvement of milk yield, comprises the steps of,
a) washing a part of fruit of Acacia leucophloea;
b) grinding the part of fruit of Acacia leucophloea;
c) mixing of the single fruit of Acacia leucophloea and 400 ml of rice water, d) heating the above mixture in a vessel containing a solvent; and
e) preparing pharmaceutically acceptable formulation thereof.
In an embodiment of the invention, the herbal compositions may be delivered as a semi-solid form or gelatinous solid, such as but not limited to paste, jelly, gel or chutney.
In an embodiment of the invention, the herbal compositions may be delivered as a gaseous form or in form of aerosols which includes but not limited to spray, fumes, inhalers or mist.
In an embodiment of the invention, the herbal compositions may also be formulated for parenteral administration by injection, which includes but is not limited to, using conventional catheterization techniques or infusion.
In an embodiment of the invention, the herbal compositions may also be formulated for topical administration, in the form of solution, cream, gel, ointment, powder, paste, gum or lozenge, using techniques known to those skilled in the formulation field. As used herein, topical administration includes delivery of the compositions to mucosal tissue of the mouth, nose and throat by, for example, spray or mist application.
In an embodiment of the invention, the herbal compositions as an active ingredient may be used alone or in combination with other therapeutic agents, for increase and improvement of milk yield in cattle.
In an embodiment of the invention, the herbal compositions of the present invention may be in the form of a decoction or may be prepared using a decoction process.
In an embodiment of the invention, the parts of Acacia leucophloea can be given chemical treatment or made to react with other chemical reagents to form mixtures or herbal compositions which are used for increase and improvement of milk yield in cattle.
In an embodiment of the invention, the desirable dose of the compositions may vary depending on the condition and the weight of the subject, severity, drug form, route and period of administration.
It is observed that when more than one herbs are used in the herbal compositions of the present invention, there is enhanced efficacy and the combination of herbs lead to synergistic effect.
The aim of this specification is to describe the invention without limiting the invention to any one embodiment or specific collection of features. A person skilled in the relevant art may realize the variations from the specific embodiments that will nonetheless fall within the scope of the invention, and such variations are deemed to be within the scope of the current invention.
It may be appreciated that various other modifications and changes may be made to the embodiment described without departing from the spirit and scope of the invention.
Example:
One whole fruit of Acacia leucophloea was sun dried and grinded to make a powder, and 50gm of this powder was mixed with rice water and offered to a cow for a period of 15 days. It was observed that there was significant increase in the milk yield of the cow.
Claims
I CLAIM:
1) Herbal compositions for increase and improvement of milk yield, the herbal compositions comprising at least a part of Acacia leucophloea.
2) Herbal compositions as claimed in claim 1, wherein said herbal compositions is selected from a group comprising whole Acacia leucophloea, extracts of Acacia leucophloea, crushed Acacia leucophloea, pulverized Acacia leucophloea, severed Acacia leucophloea, chemical constituents of Acacia leucophloea, and any combination thereof.
3) Herbal compositions as claimed in any of the preceding claims, wherein said herbal compositions further comprise additional herbs.
4) Herbal compositions as claimed in any of the preceding claims, wherein said herbal compositions further comprise additional herbs selected from Chenopodium ambrosioides seeds, Desmodium gangeticum leaves, Nigella sativa leaves, Launaea mucronata leaves, rice water, genera Centella, Withania, Pueraria, Asparagus, Chlorophytum, Boerhaavia, Sida, Saraca, Elettaria cardamomum, Piper longum, Myristica fragrans fruit, and any combination thereof.
5) Herbal compositions as claimed in any of the preceding claims, wherein the herbal compositions of the invention further comprises one or more of solvents, excipients, auxiliary agents, stabilizing agents, thickening agents, flavoring agents, fragrance, preservatives, fillers, coloring agents, and any combination thereof.
6) Herbal compositions as claimed in any of the preceding claims, wherein the herbal compositions is in forms selected from a group comprising of injectable forms; solid tablets, pellets, granules, capsules, forms; oral usage form; topical application form; semi-solid form; gaseous form; and any combination thereof.
7) A process for preparing herbal compositions for increase and improvement of milk yield, comprises the steps of,
a) washing a part of Acacia leucophloea;
i
b) grinding the part of Acacia leucophloea; and
c) preparing pharmaceutically acceptable formulation thereof.
8) A process for preparing an herbal composition as claimed in any of the preceding claims, comprise the steps of,
a) washing a part of Acacia leucophloea;
b) grinding the part of Acacia leucophloea;
c) mixing the part of Acacia leucophloea and the part of the additional herb; and d) preparing pharmaceutically acceptable formulation thereof.
9) A process for preparing an herbal composition as claimed in any of the preceding claims, comprise the steps of,
a) washing a part of fruit of Acacia leucophloea;
b) grinding the part of fruit of Acacia leucophloea;
c) mixing of the single fruit of Acacia leucophloea and rice water, and d) preparing pharmaceutically acceptable formulation thereof.
10) A process for preparing an herbal composition as claimed in any of the preceding claims, comprise the steps of,
a) washing a part of fruit of Acacia leucophloea;
b) grinding the part of fruit of Acacia leucophloea;
c) mixing of the single fruit of Acacia leucophloea and 400 ml of rice water, d) heating the above mixture in a vessel containing a solvent; and
e) preparing pharmaceutically acceptable formulation thereof.
Applications Claiming Priority (2)
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IN464KO2011 | 2011-04-01 | ||
IN464/KOL/2011 | 2011-04-01 |
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WO2012131646A2 true WO2012131646A2 (en) | 2012-10-04 |
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Cited By (1)
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CN103222563A (en) * | 2013-04-19 | 2013-07-31 | 安徽强农牧业有限公司 | Forage capable of increasing disease resistance of dairy cows, and preparation method thereof |
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NZ515869A (en) * | 2001-11-30 | 2003-09-26 | Med Chem Ingredients Ltd | Compositions and methods for animal treatment comprising gluco- and isomalto- oligosaccharides |
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CN103222563A (en) * | 2013-04-19 | 2013-07-31 | 安徽强农牧业有限公司 | Forage capable of increasing disease resistance of dairy cows, and preparation method thereof |
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