WO2012125745A2 - Detector and method for detection of h. pylori - Google Patents
Detector and method for detection of h. pylori Download PDFInfo
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- WO2012125745A2 WO2012125745A2 PCT/US2012/029103 US2012029103W WO2012125745A2 WO 2012125745 A2 WO2012125745 A2 WO 2012125745A2 US 2012029103 W US2012029103 W US 2012029103W WO 2012125745 A2 WO2012125745 A2 WO 2012125745A2
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- ammonia
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- 0 C[*-]Cc(cc(cc1)NC(C=C2)=CC=I2Nc(cc2)ccc2Nc2ccc(*(*)*)cc2)c1N*(*)* Chemical compound C[*-]Cc(cc(cc1)NC(C=C2)=CC=I2Nc(cc2)ccc2Nc2ccc(*(*)*)cc2)c1N*(*)* 0.000 description 2
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
- A61B5/097—Devices for facilitating collection of breath or for directing breath into or through measuring devices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Detecting, measuring or recording devices for evaluating the respiratory organs
- A61B5/082—Evaluation by breath analysis, e.g. determination of the chemical composition of exhaled breath
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
- A61B5/4216—Diagnosing or evaluating gastrointestinal ulcers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
- A61B2562/02—Details of sensors specially adapted for in-vivo measurements
- A61B2562/0285—Nanoscale sensors
Definitions
- TITLE DETECTOR AND METHOD FOR DETECTION OF H. PYLORI
- This invention relates to a portable, hand-held, point-of-care medical diagnostic device for the detection of Helicobacter Pylori (H. Pylori) with unlabeled urea and utilizing an ammonia specific and sensitive nanosensor constructed using a polyaniline (PANI) - carbon nanotube composite.
- H. Pylori Helicobacter Pylori
- PANI polyaniline
- H. Pylori a well-known pathogen of the human digestive tract, colonizes the gastrointestinal mucosa at any age, most commonly in childhood. H. Pylori has been associated with significant morbidity and mortality being etiologically linked to peptic ulcer disease, bleeding ulcers, gastric lymphomas (MALTOMAS) and certain forms of gastric cancer. Early diagnosis and eradication of H. Pylori contributes to improved health and prevention of one of the deadliest human cancers. The prevalence of H. Pylori in the developed world is estimated at 25%-55%, depending on demographics, and in the developing world at over 80%, the majority being children.
- H. Pylori Detection of H. Pylori has been accomplished through several diagnostic modalities. These include: a) serum testing for antibodies to H. Pylori; b) gastrointestinal mucosal biopsies for rapid urease testing; c) culture and sensitivity of H. Pylori in tissues obtained through upper gastrointestinal endoscopies; d) stool testing for H. Pylori antigen; and e) Urea Breath Test (UBT).
- Urea Breath Test for the detection of H. Pylori is based on the ability the bacterium possesses to convert urea to ammonia and CC
- UBTs There are two types of FDA approved, commercially-available UBTs based upon the type of urea substrate used: lj C labeled Urea and 14 C labeled urea.
- 14 C is a radioactive isotope of carbon and the 14 C- UBT based on detection of l C0 2 in breath is practically abandoned.
- 13 C is a stable non-radioactive isotope of carbon, encountered in nature and the 13 C-UBT based on the detection of 13 C0 2 in breath is currently the gold standard breath test for the detection of H. Pylori.
- the present invention solves above-mentioned shortcomings by using unlabeled urea as a substrate, by measuring ammonia in breath, instead of CO 2 , and by utilizing an ammonia specific and sensitive nanosensor constructed using a polyaniline (PANI) - carbon nanotube composite.
- PANI polyaniline
- the present invention provides a highly sensitive and specific ammonia sensing device for the detection of H. Pylori which is inexpensive, non-invasive, portable, point-of- care and easy to operate. . » jiviij for gastrointestinal endoscopy to obtain mucosal biopsies and culture for H. Pylori, for stool- testing for the detection of H. Pylori antigen and for the detection of H. Pylori using ⁇ C- Urea Breath Test.
- the present invention comprises an inexpensive, portable, hand held, point-of-care, non- ammonia in breath using a Dolvaniline-carbon nanotube sensor.
- Figure 1 is a block diagram of a detector according to the invention.
- Figure 2 shows the fundamental mechanism for chemical sensing using polyaniline thin films
- Figure 3 shows an image of the sensor in a sensor holder
- Figures 4(a) and (b) show sensor output as a function of pulses of ammonia in dry nitrogen, with Figure 4(a) being a magnification of the circled area (direction of the arrow) of Figure 4(b);
- Figure 5 shows change of resistance of PANI-EB sensor in linear scale on both axes
- Figure 6 shows resistance of the sensor in semi-log scale
- Figure 7 shows sensor output under a pulse of lOppm of ammonia in nitrogen
- Figure 8 shows sensor output under lOppm ammonia in nitrogen in semi-log scale
- Figure 9 shows a graph of how the PANI-EB sensor easily detects ammonia at lOppb level in linear approximation.
- Figure 10 shows electronic circuitry of a device according to the invention. DETAILED DESCRIPTION OF AN EMBODIMENT
- Figure 1 shows a breath-analyzer which consists of mouth piece, trap, emeraldme-base polyaniline (PANI-EB) sensor, N 2 flow, and electronics.
- the electronics comprise an acquisition module, a memory/computation module, and a yes/no display, and a numerical display.
- PANI-EB Emeraldine base polyaniline
- PANI-CSA emeraldine salt form
- Thin films were prepared using the spin casting technique on clean glass substrates with pre- patterned platinum electrodes. Glass substrates were cleaned via sonication in acetone followed by rinsing in deionized water. Polymer films were spin-cast at 1000 RPM for 45 seconds to produce films -100-200 nm thick. A section of the thin films were removed from the Pt finger electrodes to ensure direct electrical contact during measurements by using a combination of Oi/Ar plasma in a March Plasma RIE.
- Figure 3 shows an image of the sensor in the sensor holder.
- the whole substrate is covered with spin-casted PANI film.
- the active area is located in the center of the substrate
- the PANI-CSA thin film samples were characterized by measuring current flow as a function of exposure to ammonia vapors in nitrogen atmosphere at room and elevated temperatures (up to 70°C) at fixed applied voltage. The duration of sensor exposure to ammonia and the concentration of ammonia gas varied.
- FIG. 4(b) shows sensor output as a function of a pulse of ammonia in dry nitrogen of approximately lOppb at 100 seconds and 10 4 ppm at about 250 seconds.
- the initial stage of the sensor response in the circle corresponds to approximately lOppb, as shown in magnification in Figure 4(a), and has a sudden change of characteristic resistance in ohms from the flat baseline to a significant slope. The increase in resistance results in a drop in voltage.
- Figure 5 is a graph showing the change of resistance of the PANI-EB sensor in a linear scale on both axis in response to a pulse of 10 4 ppm of NH 3 in dry N 2 .
- Figure 6 is a graph showing resistance of the sensor in semi-log scale, and shows a two order of magnitude change of the resistance at the application of 10 4 ppm NH 3 in dry N 2 .
- Figure 7 shows a graph of sensor output under a pulse of 10 ppm of ammonia (NH 3 ) in dry nitrogen (N 2 ).
- the analog-to-digital converter (ADC) is shown (proportional to the current in the sensor, where 1 Volt corresponds to 10 U A)-
- the heating of the sensor up to 70°C was introduced for the purpose of recovery of the sensor.
- Figure 8 shows a graph of sensor output under 10 ppm of ammonia ( ⁇ ⁇ , ) in dry nitrogen (N 2 ) in semi-log scale.
- the resistance of the sensor in semi -log scale shows one order of magnitude change of the application of 10 ppm (NH 3 ) in dry (N 2 ).
- Figure 9 shows a graph of how the PANI-EB sensor easily detects ammonia at 10 ppb level.
- the PANI-EB ammonia sensor is an improvement over the M0O 3 ammonia sensor in the Urea Breath Test in the following ways:
- the PANI-EB sensor has higher specificity and sensitivity to ammonia than the M0O 3 sensor.
- the PANI-EB sensor need only be heated to 70°F in order to return to the baseline and be ready to be re-used, while the M0O 3 sensor requires heating at 470°F. Such high heat will create problems for handling a hand-held device.
- the PANI-EB sensor is very easily reproduced as compared to the M0O 3 nanosensor.
- Figure 10 shows the electronic circuitry of the device.
- Figure 10 shows a sensor, interface circuitry and display.
- a micro-controller contains the Analog-to-Digital Converter memory (SRAM), and an Arithmetic Logic Unit (ALU).
- the V test is a voltage proportional to the resistance of the sensor.
- the sensor will detect ammonia gas. If one wishes to detect gases other than ammonia, other sensors could be used. More than one sensor could be incorporated, with a switch to select connection of the sensor to the circuit for the specific gas to be detected.
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- Veterinary Medicine (AREA)
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Abstract
An inexpensive, portable, hand held, point-of-care, non-invasive breath-analyzer for the detection of H. Pylori in adults and children by measuring ammonia in breath using a polyaniline-carbon nanotube sensor.
Description
TITLE: DETECTOR AND METHOD FOR DETECTION OF H. PYLORI
CROSS REFERENCE TO RELATED APPLICATIONS
This application claims priority on U.S. Provisional Application Serial No. 61/452,391 filed March 14, 2011, which is incorporated by reference herein. This application also incorporates by reference U.S. Serial No. 61/379,963 filed September 3, 2010, U.S. Serial No. 13/226,082 filed September 6, 2011 , and U.S. Serial No. 61/452,507 filed March 14, 2011.
FIELD OF THE INVENTION
This invention relates to a portable, hand-held, point-of-care medical diagnostic device for the detection of Helicobacter Pylori (H. Pylori) with unlabeled urea and utilizing an ammonia specific and sensitive nanosensor constructed using a polyaniline (PANI) - carbon nanotube composite.
BACKGROUND OF THE INVENTION
H. Pylori, a well-known pathogen of the human digestive tract, colonizes the gastrointestinal mucosa at any age, most commonly in childhood. H. Pylori has been associated with significant morbidity and mortality being etiologically linked to peptic ulcer disease, bleeding ulcers, gastric lymphomas (MALTOMAS) and certain forms of gastric cancer. Early diagnosis and eradication of H. Pylori contributes to improved health and prevention of one of the deadliest human cancers. The prevalence of H. Pylori in the developed world is estimated at 25%-55%, depending on demographics, and in the developing world at over 80%, the majority being children.
Detection of H. Pylori has been accomplished through several diagnostic modalities. These include: a) serum testing for antibodies to H. Pylori; b) gastrointestinal mucosal biopsies for rapid urease testing; c) culture and sensitivity of H. Pylori in tissues obtained through upper gastrointestinal endoscopies; d) stool testing for H. Pylori antigen; and e) Urea Breath Test (UBT).
Serologic testing for the detection of antibodies to H. Pylori is an inefficient diagnostic tool as it cannot be used to assess treatment efficacy and eradication of H. Pylori.
Gastrointestinal endoscopy to obtain mucosal biopsies is an invasive and expensive method to detect the presence of H. Pylori in the gastrointestinal tract while stool testing for H. Pylori antigen is inefficient and cumbersome and has the lowest sensitivity and specificity of all available H. Pylori detection methods. 13C-Urea Breath Test is expensive and requires breath samples to be transported to a central lab facility for testing.
Urea Breath Test for the detection of H. Pylori is based on the ability the bacterium possesses to convert urea to ammonia and CC There are two types of FDA approved, commercially-available UBTs based upon the type of urea substrate used: ljC labeled Urea and 14C labeled urea. 14C is a radioactive isotope of carbon and the 14C- UBT based on detection of l C02 in breath is practically abandoned. 13 C is a stable non-radioactive isotope of carbon, encountered in nature and the 13C-UBT based on the detection of 13C02 in breath is currently the gold standard breath test for the detection of H. Pylori.
Limitations of widespread use of 1 C-UBT arise from the high cost of 13C-urea and of the instrument to detect Ι 3(Χ>2.
SUMMARY OF THE INVENTION
The present invention solves above-mentioned shortcomings by using unlabeled urea as a substrate, by measuring ammonia in breath, instead of CO2, and by utilizing an ammonia specific and sensitive nanosensor constructed using a polyaniline (PANI) - carbon nanotube composite.
As a result the present invention provides a highly sensitive and specific ammonia sensing device for the detection of H. Pylori which is inexpensive, non-invasive, portable, point-of- care and easy to operate.
. » jiviij for gastrointestinal endoscopy to obtain mucosal biopsies and culture for H. Pylori, for stool- testing for the detection of H. Pylori antigen and for the detection of H. Pylori using ~ C- Urea Breath Test.
The present invention comprises an inexpensive, portable, hand held, point-of-care, non- ammonia in breath using a Dolvaniline-carbon nanotube sensor.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 is a block diagram of a detector according to the invention;
Figure 2 shows the fundamental mechanism for chemical sensing using polyaniline thin films;
Figure 3 shows an image of the sensor in a sensor holder;
Figures 4(a) and (b) show sensor output as a function of pulses of ammonia in dry nitrogen, with Figure 4(a) being a magnification of the circled area (direction of the arrow) of Figure 4(b);
Figure 5 shows change of resistance of PANI-EB sensor in linear scale on both axes;
Figure 6 shows resistance of the sensor in semi-log scale;
Figure 7 shows sensor output under a pulse of lOppm of ammonia in nitrogen;
Figure 8 shows sensor output under lOppm ammonia in nitrogen in semi-log scale;
Figure 9 shows a graph of how the PANI-EB sensor easily detects ammonia at lOppb level in linear approximation; and
Figure 10 shows electronic circuitry of a device according to the invention. DETAILED DESCRIPTION OF AN EMBODIMENT
A preferred embodiment will be described, but the embodiment is merely one example of how to practice the invention, and the invention is not limited to this embodiment.
Figure 1 shows a breath-analyzer which consists of mouth piece, trap, emeraldme-base polyaniline (PANI-EB) sensor, N2 flow, and electronics. The electronics comprise an acquisition module, a memory/computation module, and a yes/no display, and a numerical display.
Technologies based on polymer thin films for electronic and chemical sensor applications are currently under developnient and are enabled by the availability of a special class of oolvmeric materials referred to as coniusrated nolvmers— or conducting polymers. These
conducting polymers are being investigated for chemical sensor applications, and initial work focuses on the use of the emeraldine forms of polyaniline (PANI), a schematic of which is shown in Figure 2. Polyaniline is particularly useful as a chemical sensor for detection of ammonia vapors even at low concentrations (ppm - ppb) range. The present invention demonstrates the effect of highly conducting additives on improving the response rate and sensitivity of the sensor. Use of polyaniline thin films and their nanocomposites enables detection of low ammonia concentrations in human breath for detection of H. Pylori.
The fundamental mechanism for chemical sensing using polyaniline thin films is shown in Figure 2 (See, Pure Appl. Chem. Vol. 74, No. 5 pp 857-02) where the emeraldine salt form of polyaniline is deprotonated to yield the less conducting emeraldine base form. The change in resistivity from highly conducting lo practically insulating is used to gauge the extent of chemical reactivity with the nitrogen bonding sites along the PANI backbone. Exposure of the PANI emeraldine salt to ammonia produces this reversible de-doping effect allowing sensors to be re-used.
Emeraldine base polyaniline (PANI-EB) with a molecular weight of 10k and camphor-10 sulphonic acid B were purchased from Aldrich and used as received. PANI-EB and CSA were dissolved in chloroform at a molar ratio of 1:0.5 to completely protonate the PANI backbone to produce the emeraldine salt form (PANI -CSA). Solutions were stirred for 3 days and sonicated for 15 minutes prior to preparing thin films.
Thin films were prepared using the spin casting technique on clean glass substrates with pre- patterned platinum electrodes. Glass substrates were cleaned via sonication in acetone followed by rinsing in deionized water. Polymer films were spin-cast at 1000 RPM for 45 seconds to produce films -100-200 nm thick. A section of the thin films were removed from the Pt finger electrodes to ensure direct electrical contact during measurements by using a combination of Oi/Ar plasma in a March Plasma RIE.
Figure 3 shows an image of the sensor in the sensor holder. The whole substrate is covered with spin-casted PANI film. The active area is located in the center of the substrate
(dimension 20x10mm) and it is 4x4mm with four parallel planar Pt electrodes with Sum separation and PANI film on the top.
The PANI-CSA thin film samples were characterized by measuring current flow as a function of exposure to ammonia vapors in nitrogen atmosphere at room and elevated
temperatures (up to 70°C) at fixed applied voltage. The duration of sensor exposure to ammonia and the concentration of ammonia gas varied.
The general-sensitivity of the sensor to ammonia was tested by applying relatively high concentration of ammonia in dry nitrogen. As shown in Figures 4-9 ammonia
concentrations correspond to 10 ppm and 10,000 ppm NH3 in N2. The results are presented in Figures 4-9.
The sensitivity of the PANI-EB sensor is significant as demonstrated by the PANI-EB sensor's ability to sense very low ppb (approximately lOppb) concentrations of ammonia. Use of this technology will optimize the experimental configuration of the PANI-EB sensor to meet the rigorous demands that testing in the ppb range requires. Figure 4(b) shows sensor output as a function of a pulse of ammonia in dry nitrogen of approximately lOppb at 100 seconds and 104ppm at about 250 seconds. The initial stage of the sensor response in the circle corresponds to approximately lOppb, as shown in magnification in Figure 4(a), and has a sudden change of characteristic resistance in ohms from the flat baseline to a significant slope. The increase in resistance results in a drop in voltage.
Figure 5 is a graph showing the change of resistance of the PANI-EB sensor in a linear scale on both axis in response to a pulse of 104 ppm of NH3 in dry N2.
Figure 6 is a graph showing resistance of the sensor in semi-log scale, and shows a two order of magnitude change of the resistance at the application of 104 ppm NH3 in dry N2.
Figure 7 shows a graph of sensor output under a pulse of 10 ppm of ammonia (NH3) in dry nitrogen (N2). The analog-to-digital converter (ADC) is shown (proportional to the current in the sensor, where 1 Volt corresponds to 10UA)- The heating of the sensor up to 70°C was introduced for the purpose of recovery of the sensor.
Figure 8 shows a graph of sensor output under 10 ppm of ammonia (ΝΗ ·, ) in dry nitrogen (N2) in semi-log scale. The resistance of the sensor in semi -log scale shows one order of magnitude change of the application of 10 ppm (NH3) in dry (N2).
Figure 9 shows a graph of how the PANI-EB sensor easily detects ammonia at 10 ppb level.
The PANI-EB ammonia sensor is an improvement over the M0O3 ammonia sensor in the Urea Breath Test in the following ways:
1. The PANI-EB sensor has higher specificity and sensitivity to ammonia than the M0O3 sensor.
2. The PANI-EB sensor need only be heated to 70°F in order to return to the baseline and be ready to be re-used, while the M0O3 sensor requires heating at 470°F. Such high heat will create problems for handling a hand-held device.
3. The PANI-EB sensor is very easily reproduced as compared to the M0O3 nanosensor.
Figure 10 shows the electronic circuitry of the device. Figure 10 shows a sensor, interface circuitry and display. A micro-controller (μθ) contains the Analog-to-Digital Converter memory (SRAM), and an Arithmetic Logic Unit (ALU). The V test is a voltage proportional to the resistance of the sensor. The sensor will detect ammonia gas. If one wishes to detect gases other than ammonia, other sensors could be used. More than one sensor could be incorporated, with a switch to select connection of the sensor to the circuit for the specific gas to be detected.
Although one preferred embodiment has been shown and described, the invention is not limited to this embodiment, and the scope is defined by reference to the following claims.
Claims
WHAT IS CLAIMED IS:
1. A detector for detecting H. Pylori, comprising a polyaniline (PANI) - carbon nanotube composite having unlabeled urea as a substrate which detects the presence of ammonia in breath, which thereby detects the presence of H. Pylori.
2. The detector according to Claim 1, wherein the detector is in the form of a hand-held, portable device.
3. The detector according to Claim 1, wherein the detector further comprises an acquisition module for receiving an output signal from the PANI nanotube composite having a characteristic indicating the detected concentration of ammonia and producing an output signal, a computation module connected to receive the output signal from the acquisition module and producing an output signal, and a display for displaying the results of the detector, based on the acquisition module output signal.
4. The detector according to Claim 3, wherein the display comprises a numerical display for displaying the concentration of detected ammonia.
5. The detector according to Claim 3, wherein the display comprises a visual indicator which displays the results of a threshold detection indicating the presence of H. Pylori above a threshold concentration.
6. A method of detecting H. Pylori, comprising using a polyaniline (PANI) - carbon nanotube composite having unlabeled urea as a substrate which detects the presence of ammonia in breath, which thereby detects the presence of H. Pylori.
7. The method according to Claim 6, further comprising using a detector in the form of a hand-held, portable device.
8. The method according to Claim 6, further comprising acquiring an output signal from the PANI nanotube composite which has a characteristic indicating the detected concentration of ammonia, using a computation module to complete the detected ammonia concentration, and displaying the results of the computation. ism: JLLL& ■
10. The method of Claim 8, comprising comparing the detected ammonia concentration to a threshold amount, and displaying whether the amount has been exceeded, or is less than the threshold.
11. A detector for detecting the presence of ammonia in breath, comprising: a hand-held portable housing;
a sensor in the housing for detecting the presence of ammonia in a concentration sufficiently low to detect, from a user's breath, the presence of H. Pylori;
an electronic circuit connected to the sensor for providing an output representative of the concentration of ammonia detected; and
an output display connected to the electronic circuit for displaying a visual signal in response to the output indicating the concentration of ammonia detected.
12. The detector according to Claim 11, wherein the detector further comprises an acquisition module for receiving an output signal from the PAN1 nanotube composite having a characteristic indicating the detected concentration of ammonia and producing an output signal, a computation module connected to receive the output signal from the acquisition module and producing an output signal, and a display for displaying the results of the detector, based on the acquisition module output signal.
13. The detector according to Claim 11, wherein the display comprises a numerical display for displaying the concentration of detected ammonia.
14. The detector according to Claim 13, wherein the display comprises a visual indicator which displays the results of a threshold detection indicating the presence of H. Pylori above a threshold concentration.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020137027085A KR20140037828A (en) | 2011-03-14 | 2012-03-14 | Detector and method for detection of h.pylori |
EP12757987.8A EP2685892A2 (en) | 2011-03-14 | 2012-03-14 | Detector and method for detection of h. pylori |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US201161452391P | 2011-03-14 | 2011-03-14 | |
US61/452,391 | 2011-03-14 |
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WO2012125745A2 true WO2012125745A2 (en) | 2012-09-20 |
WO2012125745A3 WO2012125745A3 (en) | 2013-12-12 |
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PCT/US2012/029103 WO2012125745A2 (en) | 2011-03-14 | 2012-03-14 | Detector and method for detection of h. pylori |
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EP (1) | EP2685892A2 (en) |
KR (1) | KR20140037828A (en) |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2506863A (en) * | 2012-10-09 | 2014-04-16 | Univ Dublin City | System and method for analysing and measuring ammonia levels in a sample |
US9678058B2 (en) | 2010-09-03 | 2017-06-13 | Anastasia Rigas | Diagnostic method and breath testing device |
US10401318B2 (en) | 2011-03-14 | 2019-09-03 | Anastasia Rigas | Breath analyzer and breath test methods |
WO2021021299A1 (en) * | 2019-07-26 | 2021-02-04 | Heteron Biotechnologies, Llc | Breath analyzer and urea breath test method |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040077965A1 (en) * | 2001-11-13 | 2004-04-22 | Photonic Biosystems, Inc. | Method for diagnosis of helicobacter pylori infection |
US20080077037A1 (en) * | 2003-04-21 | 2008-03-27 | Pelagia-Irene Gouma | Selective point of care nanoprobe breath analyzer |
US20080093226A1 (en) * | 2005-10-27 | 2008-04-24 | Mikhail Briman | Ammonia nanosensors, and environmental control system |
-
2012
- 2012-03-14 EP EP12757987.8A patent/EP2685892A2/en not_active Withdrawn
- 2012-03-14 KR KR1020137027085A patent/KR20140037828A/en not_active Application Discontinuation
- 2012-03-14 WO PCT/US2012/029103 patent/WO2012125745A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040077965A1 (en) * | 2001-11-13 | 2004-04-22 | Photonic Biosystems, Inc. | Method for diagnosis of helicobacter pylori infection |
US20080077037A1 (en) * | 2003-04-21 | 2008-03-27 | Pelagia-Irene Gouma | Selective point of care nanoprobe breath analyzer |
US20080093226A1 (en) * | 2005-10-27 | 2008-04-24 | Mikhail Briman | Ammonia nanosensors, and environmental control system |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9678058B2 (en) | 2010-09-03 | 2017-06-13 | Anastasia Rigas | Diagnostic method and breath testing device |
US10401318B2 (en) | 2011-03-14 | 2019-09-03 | Anastasia Rigas | Breath analyzer and breath test methods |
GB2506863A (en) * | 2012-10-09 | 2014-04-16 | Univ Dublin City | System and method for analysing and measuring ammonia levels in a sample |
WO2014056961A1 (en) * | 2012-10-09 | 2014-04-17 | Dublin City University | System and method for analysing and measuring ammonia levels in a sample |
US9435788B2 (en) | 2012-10-09 | 2016-09-06 | Dublin City University | System and method for analysing and measuring ammonia levels in a sample |
WO2021021299A1 (en) * | 2019-07-26 | 2021-02-04 | Heteron Biotechnologies, Llc | Breath analyzer and urea breath test method |
Also Published As
Publication number | Publication date |
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EP2685892A2 (en) | 2014-01-22 |
WO2012125745A3 (en) | 2013-12-12 |
KR20140037828A (en) | 2014-03-27 |
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