WO2012125737A3 - Methods for increasing carbon fixation - Google Patents

Methods for increasing carbon fixation Download PDF

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Publication number
WO2012125737A3
WO2012125737A3 PCT/US2012/029091 US2012029091W WO2012125737A3 WO 2012125737 A3 WO2012125737 A3 WO 2012125737A3 US 2012029091 W US2012029091 W US 2012029091W WO 2012125737 A3 WO2012125737 A3 WO 2012125737A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
carbon fixation
increasing carbon
transgenic plants
overexpression
Prior art date
Application number
PCT/US2012/029091
Other languages
French (fr)
Other versions
WO2012125737A2 (en
Inventor
Richard Sayre
Original Assignee
Donald Danforth Plant Science Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Donald Danforth Plant Science Center filed Critical Donald Danforth Plant Science Center
Publication of WO2012125737A2 publication Critical patent/WO2012125737A2/en
Publication of WO2012125737A3 publication Critical patent/WO2012125737A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8269—Photosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01127—[Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase (2.1.1.127)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01001—Carbonate dehydratase (4.2.1.1), i.e. carbonic anhydrase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention includes methods and transgenic plants that exhibit enhanced rates of carbon fixation and improved carbohydrate content. In one aspect, such methods and transgenic plants are created through the overexpression of membrane associated bicarbonate transporters in conjunction with a highly active carbonic anhydrase.
PCT/US2012/029091 2011-03-14 2012-03-14 Methods for increasing carbon fixation WO2012125737A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161452395P 2011-03-14 2011-03-14
US61/452,395 2011-03-14

Publications (2)

Publication Number Publication Date
WO2012125737A2 WO2012125737A2 (en) 2012-09-20
WO2012125737A3 true WO2012125737A3 (en) 2013-03-14

Family

ID=46831325

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/029091 WO2012125737A2 (en) 2011-03-14 2012-03-14 Methods for increasing carbon fixation

Country Status (1)

Country Link
WO (1) WO2012125737A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015103074A1 (en) * 2013-12-31 2015-07-09 The University Of Massachusetts Plants with enhanced photosynthesis and methods of manufacture thereof
AR100159A1 (en) 2014-04-22 2016-09-14 Du Pont GENES OF PLASID CARBON ANHYDRAINE FOR OIL INCREASE IN SEEDS WITH INCREASED DGAT EXPRESSION
ES2773601T3 (en) 2014-07-22 2020-07-13 Nmc Inc Improved carbon fixation systems in plants and algae
EP3029060A1 (en) * 2014-12-01 2016-06-08 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Genetically modified higher plants with increased photosynthesis and/or biomass production, methods and uses thereof
WO2017091309A2 (en) * 2015-10-23 2017-06-01 The University Of Massachusetts Compositions and methods for enhanced plant growth and seed yield
CN105602982B (en) * 2016-02-03 2019-10-18 昆明藻能生物科技有限公司 A kind of construction method of the hidden dinoflagellate mutant strain of Seedling height rate
WO2017136668A1 (en) * 2016-02-04 2017-08-10 Yield10 Bioscience, Inc. Transgenic land plants comprising a putative bicarbonate transporter protein of an edible eukaryotic algae
AU2018224065B2 (en) * 2017-02-22 2021-11-11 Yield10 Bioscience, Inc. Transgenic land plants comprising enhanced levels of mitochondrial transporter protein
CA3073050A1 (en) 2017-08-31 2019-03-07 Dow Agrosciences Llc Compositions and methods for expressing transgenes using regulatory elements from chlorophyll binding ab genes
GB201817792D0 (en) 2018-10-31 2018-12-19 Imperial Innovations Ltd Metal complexes for promoting growth in a photosynthetic organism

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6320101B1 (en) * 1999-06-14 2001-11-20 Yissum Research Development Company Of The Hebrew University Of Jerusalem Enhancing inorganic carbon fixation by photosynthetic organisms
US20060095981A1 (en) * 2002-05-27 2006-05-04 Rudiger Hain Method for producing plants with suppressed photorespiration and improved c02 fixation
WO2008134571A1 (en) * 2007-04-27 2008-11-06 University Of California Plant co2 sensors, nucleic acids encoding them, and methods for making and using them
US20090070901A1 (en) * 2003-05-08 2009-03-12 University Of Kentucky Research Foundation Modified rubisco large subunit n-methyltransferase useful for targeting molecules to the active-site vicinity of ribulose-1, 5-bisphosphate
WO2011139638A2 (en) * 2010-04-25 2011-11-10 Donald Danforth Plant & Science Center Enhanced carbon fixation in photosynthetic hosts

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6320101B1 (en) * 1999-06-14 2001-11-20 Yissum Research Development Company Of The Hebrew University Of Jerusalem Enhancing inorganic carbon fixation by photosynthetic organisms
US20060095981A1 (en) * 2002-05-27 2006-05-04 Rudiger Hain Method for producing plants with suppressed photorespiration and improved c02 fixation
US20090070901A1 (en) * 2003-05-08 2009-03-12 University Of Kentucky Research Foundation Modified rubisco large subunit n-methyltransferase useful for targeting molecules to the active-site vicinity of ribulose-1, 5-bisphosphate
WO2008134571A1 (en) * 2007-04-27 2008-11-06 University Of California Plant co2 sensors, nucleic acids encoding them, and methods for making and using them
WO2011139638A2 (en) * 2010-04-25 2011-11-10 Donald Danforth Plant & Science Center Enhanced carbon fixation in photosynthetic hosts

Also Published As

Publication number Publication date
WO2012125737A2 (en) 2012-09-20

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