WO2012115390A3 - Method for preparing lactulose from lactose using cellobiose 2-epimerase or n-acetyl glucosamin 2-epimerase - Google Patents

Method for preparing lactulose from lactose using cellobiose 2-epimerase or n-acetyl glucosamin 2-epimerase Download PDF

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Publication number
WO2012115390A3
WO2012115390A3 PCT/KR2012/001177 KR2012001177W WO2012115390A3 WO 2012115390 A3 WO2012115390 A3 WO 2012115390A3 KR 2012001177 W KR2012001177 W KR 2012001177W WO 2012115390 A3 WO2012115390 A3 WO 2012115390A3
Authority
WO
WIPO (PCT)
Prior art keywords
epimerase
cellobiose
lactose
lactulose
glucosamin
Prior art date
Application number
PCT/KR2012/001177
Other languages
French (fr)
Korean (ko)
Other versions
WO2012115390A9 (en
WO2012115390A2 (en
Inventor
오덕근
김영수
Original Assignee
건국대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020110016067A external-priority patent/KR101261004B1/en
Priority claimed from KR1020110081385A external-priority patent/KR101361688B1/en
Application filed by 건국대학교 산학협력단 filed Critical 건국대학교 산학협력단
Publication of WO2012115390A2 publication Critical patent/WO2012115390A2/en
Publication of WO2012115390A9 publication Critical patent/WO2012115390A9/en
Publication of WO2012115390A3 publication Critical patent/WO2012115390A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03011—Cellobiose epimerase (5.1.3.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03014—UDP-N-acetylglucosamine 2-epimerase (non-hydrolysing) (5.1.3.14)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a method for preparing lactulose from lactose using cellobiose 2-epimerase or N-acetyl glucosamin 2-epimerase, and more specifically relates to a production method for obtaining lactulose using recombinant expression vectors containing these genes, microorganisms transformed thereby, and enzymes.
PCT/KR2012/001177 2011-02-23 2012-02-16 Method for preparing lactulose from lactose using cellobiose 2-epimerase or n-acetyl glucosamin 2-epimerase WO2012115390A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020110016067A KR101261004B1 (en) 2011-02-23 2011-02-23 Method for production of lactulose from lactose using by cellobiose 2-epimerase
KR10-2011-0016067 2011-02-23
KR10-2011-0081385 2011-08-16
KR1020110081385A KR101361688B1 (en) 2011-08-16 2011-08-16 Method for production of lactulose from lactose using N-acetyl glucosamin 2-epimerase

Publications (3)

Publication Number Publication Date
WO2012115390A2 WO2012115390A2 (en) 2012-08-30
WO2012115390A9 WO2012115390A9 (en) 2012-10-18
WO2012115390A3 true WO2012115390A3 (en) 2012-12-06

Family

ID=46721305

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/001177 WO2012115390A2 (en) 2011-02-23 2012-02-16 Method for preparing lactulose from lactose using cellobiose 2-epimerase or n-acetyl glucosamin 2-epimerase

Country Status (1)

Country Link
WO (1) WO2012115390A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56102774A (en) * 1980-01-22 1981-08-17 Shikamitsu Honsha:Kk Production of food product controlling intestinal function and giving trace amount nutrients
JPH07222594A (en) * 1994-02-10 1995-08-22 Riyoushiyoku Kenkyukai Production of galactooligosaccharide
WO2006021059A1 (en) * 2004-08-24 2006-03-02 Tetra Laval Holdings & Finance S.A. A method for elimination of microorganisms present in milk

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56102774A (en) * 1980-01-22 1981-08-17 Shikamitsu Honsha:Kk Production of food product controlling intestinal function and giving trace amount nutrients
JPH07222594A (en) * 1994-02-10 1995-08-22 Riyoushiyoku Kenkyukai Production of galactooligosaccharide
WO2006021059A1 (en) * 2004-08-24 2006-03-02 Tetra Laval Holdings & Finance S.A. A method for elimination of microorganisms present in milk

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIM, YEONG SU ET AL.: "Lactulose production from lactose and fructose by a thermostable beta-galactosidase from Sulfolobus solfataricus", ENZYME AND MICROBIAL TECHNOLOGY, vol. 39, 2006, pages 903 - 908 *
MOHAMMED AIDER ET AL.: "Isomerization of lactose and lactulose production: review", TRENDS IN FOOD SCIENCE & TECHNOLOGY, vol. 18, 2007, pages 356 - 364 *

Also Published As

Publication number Publication date
WO2012115390A9 (en) 2012-10-18
WO2012115390A2 (en) 2012-08-30

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