WO2012099397A3 - 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트 - Google Patents
실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트 Download PDFInfo
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- WO2012099397A3 WO2012099397A3 PCT/KR2012/000445 KR2012000445W WO2012099397A3 WO 2012099397 A3 WO2012099397 A3 WO 2012099397A3 KR 2012000445 W KR2012000445 W KR 2012000445W WO 2012099397 A3 WO2012099397 A3 WO 2012099397A3
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- WO
- WIPO (PCT)
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- target genes
- determining
- single nucleotide
- nucleotide polymorphism
- chain reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 표적 유전자의 대립형질 특이적 프라이머를 서로 다른 튜브에 포함하고 표적 유전자에 공통적인 프로브를 각각 사용하여 실시간 중합효소 연쇄반응을 수행하여 상기 표적 유전자의 증폭산물을 생성하는 단계; 상기 각각의 튜브의 대립형질 특이적 증폭산물로부터 Ct 값을 구하는 단계; 상기 단계 (2)에서 얻어진 Ct 값으로부터 대립형질간의 ΔCt 값을 구하는 단계; 및 상기 얻어진 ΔCt 값으로부터 상기 표적 유전자의 단일 염기 다형성을 판별하는 단계를 포함하는 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법에 관한 것이다. 본 발명의 표적 유전자의 단일 염기 다형성 판별 방법은 빠른 시간 내에 원하는 SNP를 검출할 수 있다는 장점이 있다.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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KR20110004724 | 2011-01-18 | ||
KR10-2011-0004724 | 2011-01-18 |
Publications (2)
Publication Number | Publication Date |
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WO2012099397A2 WO2012099397A2 (ko) | 2012-07-26 |
WO2012099397A3 true WO2012099397A3 (ko) | 2012-12-06 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/KR2012/000445 WO2012099397A2 (ko) | 2011-01-18 | 2012-01-18 | 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트 |
Country Status (2)
Country | Link |
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KR (1) | KR20120083868A (ko) |
WO (1) | WO2012099397A2 (ko) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107760778A (zh) * | 2017-10-25 | 2018-03-06 | 广州和康医疗技术有限公司 | 一种对cyp2c9位点进行基因型检测的检测方法及其试剂盒 |
CN107653317A (zh) * | 2017-11-16 | 2018-02-02 | 黑龙江迪安医学检验所有限公司 | 一种分子信标探针检测人类cyp2c9基因多态性的试剂盒、方法及其应用 |
KR102084965B1 (ko) * | 2018-02-28 | 2020-03-05 | (주)제노텍 | 정성적 또는 정량적 돌연변이 유전형 분석방법 및 이 방법을 수행하기 위한 실시간 pcr 키트 |
CN108929904A (zh) * | 2018-07-26 | 2018-12-04 | 山东德诺生物科技有限公司 | 用于检测rs1057910的引物探针组及其应用 |
CN110819709A (zh) * | 2019-12-16 | 2020-02-21 | 北京和合医学诊断技术股份有限公司 | 用于荧光定量pcr检测cyp2c9和vkorc1基因多态性的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7297498B2 (en) * | 2005-11-21 | 2007-11-20 | Matsushita Electric Industrial Co., Ltd. | Discrimination method of target base in DNA, and allele specific primer used in the method of the same |
KR20100031188A (ko) * | 2008-09-12 | 2010-03-22 | 주식회사 엘지생명과학 | 결핵균군 또는 마이코박테리아 속 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법 |
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2012
- 2012-01-18 KR KR1020120005935A patent/KR20120083868A/ko not_active Application Discontinuation
- 2012-01-18 WO PCT/KR2012/000445 patent/WO2012099397A2/ko active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7297498B2 (en) * | 2005-11-21 | 2007-11-20 | Matsushita Electric Industrial Co., Ltd. | Discrimination method of target base in DNA, and allele specific primer used in the method of the same |
KR20100031188A (ko) * | 2008-09-12 | 2010-03-22 | 주식회사 엘지생명과학 | 결핵균군 또는 마이코박테리아 속 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법 |
Non-Patent Citations (3)
Title |
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AOMORI T. ET AL.: "Rapid single-nucleotide polymorphism detection of cytochrome p450 (CYP2C9) and vitamin K epoxide reductase(VKORCl) genes for the warfarin dose adjustment by the smart-amplification process version 2", CLINICAL CHEMISTRY, vol. 55, 30 January 2009 (2009-01-30), pages 804 - 812 * |
GERMER S. ET AL.: "High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR", GENOME RES, vol. 10, February 2000 (2000-02-01), pages 258 - 266, XP000927195, DOI: doi:10.1101/gr.10.2.258 * |
MATTARUCCHI E. ET AL.: "Different real time PCR approaches for the fine quantification of SNP's alleles in DNA pools: assays development, characterization and pre-validation", J BIOCHEM MOL BIO, SEPTEMBER, vol. 38, 2005, pages 555 - 562 * |
Also Published As
Publication number | Publication date |
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WO2012099397A2 (ko) | 2012-07-26 |
KR20120083868A (ko) | 2012-07-26 |
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