WO2012099397A3 - 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트 - Google Patents

실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트 Download PDF

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WO2012099397A3
WO2012099397A3 PCT/KR2012/000445 KR2012000445W WO2012099397A3 WO 2012099397 A3 WO2012099397 A3 WO 2012099397A3 KR 2012000445 W KR2012000445 W KR 2012000445W WO 2012099397 A3 WO2012099397 A3 WO 2012099397A3
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WIPO (PCT)
Prior art keywords
target genes
determining
single nucleotide
nucleotide polymorphism
chain reaction
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PCT/KR2012/000445
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English (en)
French (fr)
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WO2012099397A2 (ko
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박한오
김동식
서진철
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(주) 바이오니아
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Publication of WO2012099397A2 publication Critical patent/WO2012099397A2/ko
Publication of WO2012099397A3 publication Critical patent/WO2012099397A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 표적 유전자의 대립형질 특이적 프라이머를 서로 다른 튜브에 포함하고 표적 유전자에 공통적인 프로브를 각각 사용하여 실시간 중합효소 연쇄반응을 수행하여 상기 표적 유전자의 증폭산물을 생성하는 단계; 상기 각각의 튜브의 대립형질 특이적 증폭산물로부터 Ct 값을 구하는 단계; 상기 단계 (2)에서 얻어진 Ct 값으로부터 대립형질간의 ΔCt 값을 구하는 단계; 및 상기 얻어진 ΔCt 값으로부터 상기 표적 유전자의 단일 염기 다형성을 판별하는 단계를 포함하는 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법에 관한 것이다. 본 발명의 표적 유전자의 단일 염기 다형성 판별 방법은 빠른 시간 내에 원하는 SNP를 검출할 수 있다는 장점이 있다.
PCT/KR2012/000445 2011-01-18 2012-01-18 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트 WO2012099397A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20110004724 2011-01-18
KR10-2011-0004724 2011-01-18

Publications (2)

Publication Number Publication Date
WO2012099397A2 WO2012099397A2 (ko) 2012-07-26
WO2012099397A3 true WO2012099397A3 (ko) 2012-12-06

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PCT/KR2012/000445 WO2012099397A2 (ko) 2011-01-18 2012-01-18 실시간 중합효소 연쇄반응을 이용한 표적 유전자의 단일 염기 다형성 판별 방법 및 이를 이용한 표적 유전자의 단일 염기 다형성 판별 키트

Country Status (2)

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KR (1) KR20120083868A (ko)
WO (1) WO2012099397A2 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107760778A (zh) * 2017-10-25 2018-03-06 广州和康医疗技术有限公司 一种对cyp2c9位点进行基因型检测的检测方法及其试剂盒
CN107653317A (zh) * 2017-11-16 2018-02-02 黑龙江迪安医学检验所有限公司 一种分子信标探针检测人类cyp2c9基因多态性的试剂盒、方法及其应用
KR102084965B1 (ko) * 2018-02-28 2020-03-05 (주)제노텍 정성적 또는 정량적 돌연변이 유전형 분석방법 및 이 방법을 수행하기 위한 실시간 pcr 키트
CN108929904A (zh) * 2018-07-26 2018-12-04 山东德诺生物科技有限公司 用于检测rs1057910的引物探针组及其应用
CN110819709A (zh) * 2019-12-16 2020-02-21 北京和合医学诊断技术股份有限公司 用于荧光定量pcr检测cyp2c9和vkorc1基因多态性的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297498B2 (en) * 2005-11-21 2007-11-20 Matsushita Electric Industrial Co., Ltd. Discrimination method of target base in DNA, and allele specific primer used in the method of the same
KR20100031188A (ko) * 2008-09-12 2010-03-22 주식회사 엘지생명과학 결핵균군 또는 마이코박테리아 속 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7297498B2 (en) * 2005-11-21 2007-11-20 Matsushita Electric Industrial Co., Ltd. Discrimination method of target base in DNA, and allele specific primer used in the method of the same
KR20100031188A (ko) * 2008-09-12 2010-03-22 주식회사 엘지생명과학 결핵균군 또는 마이코박테리아 속 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법

Non-Patent Citations (3)

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Title
AOMORI T. ET AL.: "Rapid single-nucleotide polymorphism detection of cytochrome p450 (CYP2C9) and vitamin K epoxide reductase(VKORCl) genes for the warfarin dose adjustment by the smart-amplification process version 2", CLINICAL CHEMISTRY, vol. 55, 30 January 2009 (2009-01-30), pages 804 - 812 *
GERMER S. ET AL.: "High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR", GENOME RES, vol. 10, February 2000 (2000-02-01), pages 258 - 266, XP000927195, DOI: doi:10.1101/gr.10.2.258 *
MATTARUCCHI E. ET AL.: "Different real time PCR approaches for the fine quantification of SNP's alleles in DNA pools: assays development, characterization and pre-validation", J BIOCHEM MOL BIO, SEPTEMBER, vol. 38, 2005, pages 555 - 562 *

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KR20120083868A (ko) 2012-07-26

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