WO2012081911A3 - Saliva purifying method - Google Patents

Saliva purifying method Download PDF

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Publication number
WO2012081911A3
WO2012081911A3 PCT/KR2011/009639 KR2011009639W WO2012081911A3 WO 2012081911 A3 WO2012081911 A3 WO 2012081911A3 KR 2011009639 W KR2011009639 W KR 2011009639W WO 2012081911 A3 WO2012081911 A3 WO 2012081911A3
Authority
WO
WIPO (PCT)
Prior art keywords
saliva
present
purifying method
purify
analyses
Prior art date
Application number
PCT/KR2011/009639
Other languages
French (fr)
Korean (ko)
Other versions
WO2012081911A2 (en
Inventor
박성대
Original Assignee
퍼팩트코리아(주)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020100127671A external-priority patent/KR20110068906A/en
Priority claimed from KR1020110133597A external-priority patent/KR101467371B1/en
Application filed by 퍼팩트코리아(주) filed Critical 퍼팩트코리아(주)
Publication of WO2012081911A2 publication Critical patent/WO2012081911A2/en
Publication of WO2012081911A3 publication Critical patent/WO2012081911A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition

Abstract

The present invention relates to a method which denatures proteins contained in the saliva by suddenly varying a temperature and pH, to thereby purify the saliva. Particularly, the method of the present invention has higher accuracy compared to conventional saliva pre-treatment methods, and thus enables very small amount of hormone in the saliva to be detected, and is therefore very useful. That is, the method of the present invention may be valuably used in a pre-treatment process for a variety of analyses which use saliva as a sample.
PCT/KR2011/009639 2010-12-14 2011-12-14 Saliva purifying method WO2012081911A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR1020100127671A KR20110068906A (en) 2009-12-15 2010-12-14 A method of diagnosing a menstrual activation using saliva
KR10-2010-0127671 2010-12-14
KR10-2011-0133597 2011-12-13
KR1020110133597A KR101467371B1 (en) 2010-12-14 2011-12-13 A Method of Purifying a Saliva

Publications (2)

Publication Number Publication Date
WO2012081911A2 WO2012081911A2 (en) 2012-06-21
WO2012081911A3 true WO2012081911A3 (en) 2012-09-07

Family

ID=46245226

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/009639 WO2012081911A2 (en) 2010-12-14 2011-12-14 Saliva purifying method

Country Status (1)

Country Link
WO (1) WO2012081911A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060183170A1 (en) * 2002-09-09 2006-08-17 Gc Corporation Pretreatment kit for saliva and pretreatment method for saliva
JP2009085946A (en) * 2007-09-12 2009-04-23 Kao Corp Quantification method for steroid hormone

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060183170A1 (en) * 2002-09-09 2006-08-17 Gc Corporation Pretreatment kit for saliva and pretreatment method for saliva
JP2009085946A (en) * 2007-09-12 2009-04-23 Kao Corp Quantification method for steroid hormone

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AKUTSU, N. ET AL.: "Measurement of estradiol levels insaliva collected from females in different menstrual phases and at different times of the day.", THE AUTONOMIC NERVOUS SYSTEMS, vol. 42, 2005 *
YEHUDA, R. ET AL.: "Relationship between 24-hour urinary-free cortisol excretion and salivary cortisol levels sampled from awakening to bedtime in healthy subjects.", LIFE SCIENCES, vol. 73, 2003 *

Also Published As

Publication number Publication date
WO2012081911A2 (en) 2012-06-21

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