WO2012081908A3 - 포도상구균의 테이코산에 대한 ｒｎａ 앱타머 - Google Patents

포도상구균의 테이코산에 대한 ｒｎａ 앱타머 Download PDF

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Publication number
WO2012081908A3
WO2012081908A3 PCT/KR2011/009631 KR2011009631W WO2012081908A3 WO 2012081908 A3 WO2012081908 A3 WO 2012081908A3 KR 2011009631 W KR2011009631 W KR 2011009631W WO 2012081908 A3 WO2012081908 A3 WO 2012081908A3
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WO
WIPO (PCT)
Prior art keywords
teiocoic
acid
staphylococcus aureus
rna aptamer
staphylococcal
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Ceased
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PCT/KR2011/009631
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English (en)
French (fr)
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WO2012081908A2 (ko
Inventor
조용진
김철진
김남수
김종태
맹진수
이성욱
이영주
한승렬
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Korea Food Research Institute KFRI
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Korea Food Research Institute KFRI
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Priority to KR1020137007652A priority Critical patent/KR101508219B1/ko
Publication of WO2012081908A2 publication Critical patent/WO2012081908A2/ko
Publication of WO2012081908A3 publication Critical patent/WO2012081908A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7115—Nucleic acids or oligonucleotides having modified bases, i.e. other than adenine, guanine, cytosine, uracil or thymine
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/44—Staphylococcus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

[요약] 본 발명은 포도상구균의 테이코산에 대한 ＲＮＡ 앱타머에 관한 것으로서, 보다 상세하게는 포도상구균의 테이코산에 특이적으로 결합하고, 그 염기 중 우라실(U) 및 시토신(C)의 2' 히드록실기가 플루오르기로 치환되어 있는 서열번호 1의 염기서열을 가짐으로써 식중독 등의 주요 원인이 되는 포도상구균의 검출에 유용하고, 포도상구균에 특이적으로 결합하여 그 작용을 억제함으로써 식품의 부패를 방지할 수 있으며, 포도상구균성 폐렴, 패혈증, 골수염, 포도상구균장염, 식중독, 포도상구균성 열상유사증후군(SSSS) 및 독소성 충격증후군(TSS)으로 이루어진 군에서 선택된 질환의 예방 및 치료에 유용한 RNA 앱타머에 관한 것이다. [대표도] 도 6
PCT/KR2011/009631 2010-12-17 2011-12-14 포도상구균의 테이코산에 대한 ｒｎａ 앱타머 Ceased WO2012081908A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020137007652A KR101508219B1 (ko) 2010-12-17 2011-12-14 포도상구균의 테이코산에 대한 ｒｎａ 앱타머

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0129767 2010-12-17
KR1020100129767A KR20120068235A (ko) 2010-12-17 2010-12-17 포도상구균의 테이코산에 대한 ｒｎａ 앱타머

Publications (2)

Publication Number Publication Date
WO2012081908A2 WO2012081908A2 (ko) 2012-06-21
WO2012081908A3 true WO2012081908A3 (ko) 2012-10-04

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PCT/KR2011/009631 Ceased WO2012081908A2 (ko) 2010-12-17 2011-12-14 포도상구균의 테이코산에 대한 ｒｎａ 앱타머

Country Status (2)

Country Link
KR (2) KR20120068235A (ko)
WO (1) WO2012081908A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103048369A (zh) * 2013-01-18 2013-04-17 江南大学 一种基于还原氧化石墨烯-纳米金复合材料的金黄色葡萄球菌无标记电化学适配体传感器

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101439158B1 (ko) * 2012-07-17 2014-09-18 한국식품연구원 식중독균 검출 키트 및 이를 이용한 식중독균 검출 방법
CA3152415A1 (en) * 2014-02-18 2015-08-27 Somalogic Operating Co., Inc. Compositions and methods for detecting microorganisms
RU2628800C2 (ru) * 2014-03-12 2017-08-22 Общество С Ограниченной Ответственностью "Фарминтерпрайсез" Амидные соединения, способы получения, применение в качестве средств для лечения и профилактики заболеваний, вызываемых рнк-содержащими вирусами
CN105483133B (zh) * 2015-11-26 2018-07-06 中国人民解放军南京军区福州总医院 金黄色葡萄球菌中毒性休克毒素-1核酸适配体t-7及其制备方法和应用
EP4288544A1 (en) 2021-02-04 2023-12-13 Fresenius Medical Care Holdings, Inc. Lipoteichoic acid (lta) aptamers and associated methods

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100227810A1 (en) * 2009-03-03 2010-09-09 Camille Cyncynatus Method for diagnosing staphylococcal infections

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100227810A1 (en) * 2009-03-03 2010-09-09 Camille Cyncynatus Method for diagnosing staphylococcal infections

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KOLBERG, J. ET AL.: "Detection of the phosphorylcholine epitope in sterptococci, Haemophilus and pathogenic Neisseriae by immunoblotting", MICROBIAL PATHOGENESIS., vol. 22, no. 6, June 1997 (1997-06-01), pages 321 - 329 *
TADLER, M. B ET AL.: "Sandwich immunoassay for the detection oflipoteichoic acid", JOURNAL OF CLINICAL LABORATORY ANALYSIS., vol. 3, no. 1, 1989, pages 21 - 25 *
WHEAT, L. J. ET AL.: "Solid-phase radioimmunoassay for detection of staphylococcal antigen in serum of rabbits with endocraditis due to Staphylococcus aureus", JOURNAL OF INFECTIOUS DISEASE., vol. 138, no. 2, August 1978 (1978-08-01), pages 174 - 180 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103048369A (zh) * 2013-01-18 2013-04-17 江南大学 一种基于还原氧化石墨烯-纳米金复合材料的金黄色葡萄球菌无标记电化学适配体传感器

Also Published As

Publication number Publication date
WO2012081908A2 (ko) 2012-06-21
KR20120068235A (ko) 2012-06-27
KR20130063013A (ko) 2013-06-13
KR101508219B1 (ko) 2015-04-07

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