WO2012074285A2 - Agent for stabilizing peptides or proteins - Google Patents

Abstract

The present invention relates to a composition for stabilizing peptides or proteins which comprises at least one selected from the group consisting of panthenol, polyalcohol ester-based compound, casein, and derivatives thereof. In addition, the invention relates to a composition comprising at least one selected from the group consisting of: at least one of peptides or proteins; and at least one of panthenol, polyalcohol ester-based compound, casein, and derivatives thereof. Furthermore, the invention relates to a method for stabilizing peptides or proteins, which comprises a step for preparing a composition by mixing at least one of peptides or proteins with at least one selected from the group consisting of panthenol, polyalcohol ester-based compound, casein, and derivatives thereof.

Description

Peptide or Protein Stabilizer

The present invention relates to agents that stabilize peptides or proteins.

Proteins are high-molecular organic compounds that make up the body of every organism. It is important as a constituent of the body of an organism and as a catalyst for various chemical reactions in cells. Based on these characteristics, proteins are widely used in various fields such as food, medicine, or cosmetics.

The present invention seeks to provide a peptide or protein stabilizer and a composition in which the peptide or protein remains stable. The present invention also provides a method of stabilizing a peptide or protein.

One aspect of the present invention provides a peptide or protein stabilizer comprising at least one selected from the group consisting of panthenol, polyalcohol ester compounds, casein, and derivatives thereof.

Another aspect of the invention provides at least one of peptides and proteins; And it provides a composition comprising at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof.

Another aspect of the invention provides at least one of peptides and proteins; And it provides a peptide or protein stabilization method comprising the step of preparing a composition by mixing at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof.

At least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof of the present invention is excellent in stabilizing peptides or proteins. The composition comprising at least one of the peptides and proteins according to one aspect of the invention comprises at least one selected from the group consisting of panthenol, polyalcohol ester compounds, casein and derivatives thereof, so that the peptide or protein is not degraded for a long time. Can be kept stable. In particular, in the case of a composition containing a peptide or protein in a dissolved state, by including one or more selected from the group consisting of panthenol, polyalcohol ester compound, casein, and derivatives thereof, since the peptide or protein is hardly decomposed, immediately before use There is no need to dissolve the peptide or protein and it is easy to store and distribute with the peptide or protein included.

Peptides or proteins are used in various fields, such as food, medicine or cosmetics, but they are unstable under external physical and chemical conditions, especially at high temperature, and are not easy to formulate. In addition, since the peptide or protein is easily dissolved when dissolved, it is inconvenient to dissolve immediately before use if the peptide or protein is to be used. In order to solve this problem, attempts have been made to include peptides such as sucrose, trehalose, fructose and mannitol in a composition containing at least one of peptides and proteins to stabilize peptides or proteins, but the degree of effect is satisfactory. no.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

One aspect of the present invention provides a peptide or protein stabilizer comprising one or more selected from the group consisting of panthenol, polyalcohol ester compounds, casein and derivatives thereof. The at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof can physically and chemically protect the peptide or protein to prevent the peptide or protein from being degraded, thereby keeping the peptide or protein stable for a long time. .

One aspect of the invention is at least one of a peptide and a protein; And it provides a composition comprising at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof. The composition comprises at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof, so that the peptide or protein contained therein can be stably maintained without prolonged degradation.

One aspect of the invention is at least one of a peptide and a protein; And it provides a peptide or protein stabilization method comprising the step of preparing a composition by mixing at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof. In this way, the peptide or protein is mixed with one or more selected from the group consisting of panthenol, polyalcohol ester compound, casein, and derivatives thereof, so that the peptide or protein can be stably maintained without degradation for a long time.

In this specification, panthenol is an alcohol derivative of pantothenic acid, which is one of the vitamin B groups, and may be represented by the following Chemical Formula 1.

Formula 1

As used herein, the derivative of panthenol includes all compounds that can be obtained by chemically changing a part of panthenol, and includes hydrogen or a specific atomic group all substituted with another atom or atomic group. More specifically, derivatives of panthenol include allantoin calcium pantothenate, calcium pantothenate, ethyl dicarboethoxy pantothenate (C ₁₇ H ₂₉ NO ₉ , formula 2), Hydroxyethyl Pantothenamide MEA (C ₁₃ H ₂₆ N ₂ O ₆ , Formula 3), Pantothenamide MEA, C ₁₁ H ₂₂ N ₂ O ₅ , Formula 4), Pantothenic Acid , C ₉ H ₁₇ NO ₅ , Formula 5), Pantothenoyl Dipeptide-13, Sodium Pantothenate, PanthenylEthylether, PanthenylEthylether acetate, C ₁₃ H ₂₅ NO ₅ , formula 6) and panthenyl triacetate (C ₁₅ H ₂₅ NO ₇ , formula 7).

Formula 2

Formula 3

Formula 4

Formula 5

Formula 6

Formula 7

In the present specification, a polyalcohol ester compound refers to a compound including a polyalcohol ester group. In one aspect of the present invention, the polyalcohol ester compound and derivatives thereof include a compound in which the ester of polyalcohol and stearic acid is esterified, and specifically, polyglyceryl stearate, polyglyceryl distearate, PEG stearate, PEG distearate, polyglyceryl methylglucose distearate or PEG methyl glucose distearate, and more particularly polyglyceryl-3 methylglucose distearate or PEG-20 methyl glucose distearate do. In another aspect of the present invention, the polyalcohol ester compound includes a compound that acts as a surfactant.

In this specification, a casein derivative is a concept including all compounds that can be obtained by chemically changing a part of casein. In one aspect of the invention, the casein or derivative thereof includes a casein salt such as casein hydrolysate or casein sodium hydrolyzed casein with an acid or enzyme. In one aspect of the invention, the casein or derivative thereof may stabilize other proteins included in the composition in addition to itself.

In one aspect of the invention, a peptide refers to a polymerized molecule of amino acids, from 2 to 10 amino acids are linked, it is a concept including a polypeptide of 10 to 20 or more than 20 linked. In another aspect of the present invention, the peptide comprises about 10 amino acids linked.

In one aspect of the invention, proteins generally include all substances that can be classified as proteins.

In one aspect of the invention, the peptide or protein may refer to a potent ingredient generally included in a pharmaceutical, cosmetic or food composition. In another aspect of the invention, peptides or proteins include those extracted from animals or plants. In another aspect of the invention, the peptide or protein comprises one or more of growth factors, hormones, interferons, vaccines, recombinant proteins, recombinant peptides and enzymes. More specifically, the peptide or protein may include epidermal growth factor, basic fibroblast growth factor, platelet derived growth factor, and vascular endothelial growth factor. ), Tyrosine Kinase Ligand, Interleukin 1 Alpha, Interleukin 1 Beta, Interleukin 17 Alpha, CC Motif Chemokine Ligand 5 (CC Motif) Chemokine Ligand 5), Human Growth Hormone, Insulin, Acyl Coenzyme A Desaturase, Albumin (Albumin), Alkaline Phosphatase, Aminopeptidase, Amylase (Amylase), Amyloglucosidase, Apo-Lactoferrin, Arthrospira Extract, Bromelain, Casein, Catalase, Cellulase, Cellulase, Collagen, Crystallins, Cytochrome C, Deoxyribonuclease, Elastin Elastin, Fibronectin, Ficin, Fish Plasma Protein, Galactose Dehydrogenase, Gelatin, Gelidium Pulchrum Protein, Glia Gliadin, Glucose Oxidase, Glycoproteins, Hemoglobin, Hesperidinase, Horsadish Peroxidase, Human Placenta Enzyme, Human Enzyme, Enzyme Placental Enzyme Placenta Protein, Human Placental Protein, Hyaluronidase, Hydroxysteroid Oxygen idoreductase, Kallikrein, Keratin, Lacase, Lactate Dehydrogenase, Lactis Proteinum, Lactoferrin, Lactoglobulin , Lactoperoxidase, Ligninase, Ligninase, Lipase, Lupineus Albus Protein, Lysozyme, Lysozyme Beta-Glucan, Macrosistis Macrocystis Pyrifera (Kelp) Protein, Milk Protein, Nacre Protein, Oryza Sativa (Rice) Bran Protein, Orissa Oryza Sativa (Rice) Seed Protein, Oxido Reductases, Pancreatin, Papain, Papain Beta-Glucan, Pepsin ), Protease, Prunus Prunus Amygdalus Dulcis (Sweet Almond) Protein, r-Clostridium Histolyticum Collagenase, Serralysin, Serum Albumin Albumin, Serum Protein, Silk, Soluble Elastin, Soluble Keratin, Soluble Proteoglycan, Soybean Peroxidase, Subtilisin ), Sutilains, Trypsin, Tyrosinase, Urease, Whey Protein, Zein, Fibroblast Growth Factor, FGF-1, FGF -2, FGF-7, FGF-10, Insulin-like growth factor-1 (IGF-1), IGF-2, Keratinocyte Growth Factor (KGF), KGF-2, β-NGF (Nerve Growth factor), Protein Placental Growth Factor-1CG (Protein PIGF-1CG), Converting Growth Factor (TGF) -α, TGF-beta-1, TGF-3, Thioredoxin, Stem Cell Factor, Tissue Inhibitors of Il-2, IL-10, IL-4, Metalloproteinase-2 Inhibitor of Metalloproteinases-2), Placenta Growth Factor, Fetal Liver Kinase-2, Superoxide Dismutase, Tripartite Motif-Containing Protein, Thymosin, Human Beta Defensin, botulinum toxin, commonly referred to as Botox ^® , and polio, diphtheria, pertussis, measles, rubella, typhoid fever, paratyphoid fever, cholera, plague, tuberculosis, One selected from the group consisting of antigens used for vaccines against tetanus, yato disease, staphylococcus or streptococci, chronic infections, tetanus, flu, yellow fever, encephalitis, roche erythematosus, rash typhus or hepatitis B Including the above, It is not limited to this. In another aspect of the invention, when the casein or derivative thereof is selected from the group consisting of panthenol, polyalcohol ester compounds, casein and derivatives thereof, at least one selected from peptides and proteins does not comprise casein or milk protein .

In one aspect of the invention, the composition comprising the peptide or protein is panthenol depending on the storage conditions such as the content of the peptide or protein, the viscosity of the composition comprising the peptide or protein or the storage temperature and duration of the composition comprising the peptide or protein, etc. , Polyalcohol ester compounds, casein and derivatives thereof may include one or more selected from the group consisting of various contents. More specifically, at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof is 0.1 to 50% by weight, specifically 0.5 to 30% by weight, and more specifically 1 to 20% by weight, based on the total weight of the composition. It may include. When included in the weight percent, not only is it appropriate to exhibit the intended effect of the present invention, but also satisfies both the stability and safety of the composition, it may be appropriate to include in the above range in terms of cost-effectiveness.

In one aspect of the invention, the weight ratio of at least one of the peptides and proteins and at least one selected from the group consisting of panthenol, polyalcohol ester compounds, casein and derivatives thereof is 50,000,000: 1 to 1: 1 or 1: 1: 1 to 50,000,000 For example, it may be 10,000,000: 1 to 1: 1 or 1: 1 to 1: 10,000,000, more specifically 500,000: 1 to 1: 1 or 1: 1 to 500,000. When included in the weight ratio, not only is it appropriate to exhibit the intended effect of the present invention, but also satisfies both the stability and safety of the composition, and may be appropriate in terms of cost-effectiveness.

 In one aspect of the invention, a composition comprising at least one of a peptide and a protein may have a viscosity of 5,000 cP to 30,000 cP. In another aspect of the invention, a composition comprising one or more of peptides and proteins may have a viscosity of 8,000 cP to 25,000 cP. In another aspect of the invention, a composition comprising one or more of peptides and proteins may have a viscosity of 10,000 cP to 20,000 cP. When a composition comprising at least one of a peptide and a protein has a viscosity in the above range, the peptide or protein stabilizing effect of panthenol, a polyalcohol ester compound, casein or a derivative thereof can be exerted better.

Peptide or protein stabilization method according to an aspect of the present invention is one or more of peptides and proteins; And mixing at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein, and derivatives thereof to prepare the composition, wherein the prepared composition is -30 ° C to 30 ° C, specifically -24 ° C to 4 ° C. The freezing conditions and the refrigeration conditions of -10 ℃ to 10 ℃, may further comprise the step of storing at room temperature conditions of 4 ℃ to 30 ℃.

One aspect of the invention provides a cosmetic, pharmaceutical or food composition comprising a peptide or protein stabilizer comprising at least one selected from the group consisting of panthenol, polyalcohol ester compounds, casein and derivatives thereof. Another aspect of the invention provides at least one of peptides and proteins; And it provides a cosmetic, pharmaceutical or food composition comprising at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof. Another aspect of the invention provides at least one of peptides and proteins; And it provides a peptide or protein stabilization method comprising the step of preparing a cosmetic, pharmaceutical or food composition by mixing at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof.

Cosmetic compositions according to the invention may be provided in all formulations suitable for topical application. For example, emulsions obtained by dispersing the oil phase in solutions, gels, solids, pastes, anhydrous products, water phases, emulsions obtained by dispersing the oil phases in water, suspensions, powders, pastes, microneedles, foams or aerosol compositions. It may be provided in the form of. These compositions can be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may include other ingredients in addition to the above-mentioned substances within the range not impairing the main effect, preferably giving a synergistic effect to the main effect. In addition, the cosmetic composition according to the present invention may further include a moisturizer, an emulsifier, an ultraviolet absorber, a preservative, a fungicide, an antioxidant, a pH adjuster, organic and inorganic pigments, flavors, coolants, or limiting agents. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.

The pharmaceutical composition according to an aspect of the present invention is not limited to the treatment of a particular disease, and includes a composition that can be used for medicinal purposes including peptides or proteins, and examples thereof include pharmaceutical compositions for promoting skin regeneration and wound healing. It is not limited.

The pharmaceutical composition according to one aspect of the present invention may be administered orally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Formulations for oral administration may be tablets, pills, soft and hard capsules, granules, powders, granules, solutions, emulsions or pellets, but are not limited thereto. It is not. These formulations may contain, in addition to the active ingredient, diluents (e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose or glycine), glidants (e.g. silica, talc, stearic acid or polyethylene glycol), or binders (e.g. magnesium aluminum Silicates, starch pastes, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose or polyvinylpyrrolidine). It may optionally contain pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, or sweeteners. The tablets can be prepared by conventional mixing, granulating or coating methods.

Formulations for parenteral administration may be, but are not limited to, injections, drops, lotions, ointments, gels, creams, suspensions, emulsions, suppositories, patches or sprays.

The active ingredient of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Dosage determination based on these factors is within the level of one skilled in the art and its daily dosage may be, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / kg. May be, but is not limited to.

The formulation of the food composition according to one aspect of the present invention is not particularly limited, but may be, for example, formulated into tablets, granules, drinks, caramels, diet bars and the like. In addition to the active ingredient, the food composition of each formulation may be suitably selected by a person skilled in the art according to the formulation or purpose of use in addition to the active ingredient, and may be synergistic when applied simultaneously with other raw materials.

The determination of the dosage of the active ingredient is within the level of those skilled in the art, and its daily dosage may vary depending on various factors such as the age, health condition, complications, etc. of the subject to be administered.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples, Comparative Examples and Experimental Examples. However, these Examples, Comparative Examples and Experimental Examples are provided only for the purpose of illustration in order to help the understanding of the present invention, but the scope and scope of the present invention is not limited thereto.

Example 1 and Comparative Examples 1 to 5 Preparation of Cosmetic Composition

The cosmetic composition (emulsion) of Example 1 and Comparative Examples 1 to 5 was prepared according to the configuration of Table 1 below (unit: wt%). Example 1 includes panthenol, and Comparative Examples 1-4 comprise sugars.

To explain the production method in more detail, the mixture was sufficiently mixed at 7000 rpm for 3 minutes while first adding an oil phase component to the aqueous phase component. Then, the epidermal growth factor solution was added and mixed well by homomixing for 1-2 minutes.

Table 1

ingredient	Example 1	Comparative Example 1	Comparative Example 2	Comparative Example 3	Comparative Example 4	Comparative Example 5
Squalane	5	5	5	5	5	5
100 ppm solution of epidermal growth factor	One	One	One	One	One	One
DL-panthenol	10	-	-	-	-	-
Trehalos	-	10	-	-	-	-
Sucrose	-	-	10	-	-	-
Xylitol	-	-	-	10	-	-
Mannitol	-	-	-	-	10	-
Carbomer	2	2	2	2	2	2
Sodium hydroxide	0.2	0.2	0.2	0.2	0.2	0.2
Distilled water	81.8	81.8	81.8	81.8	81.8	81.8

Experimental Example 1 Evaluation of Protein Stabilization of Panthenol

In order to determine the stability of the epidermal growth factor contained in the composition of Example 1 and Comparative Examples 1 to 5, each composition was stored at 4 ° C, 25 ° C, and 50 ° C, and the content thereof was determined by ELISA (Enzyme-Linked ImmunoSorbent Assay). ) Was confirmed by a measurement method. Measure the content value when stored at 4 ℃ and 25 ℃ for 2 weeks, then divide the content value after 2 weeks of storage at 4 ℃ from 2 weeks after storage at 25 ℃ to obtain relative thermal stability (%). After obtaining, it is shown in the table below.

TABLE 2

sample	Relative Thermal Stability (%)
Example 1	94.0
Comparative Example 1	45.4
Comparative Example 2	30.7
Comparative Example 3	16.5
Comparative Example 4	32.8
Comparative Example 5	10.4

As can be seen from the above results, the stability of epidermal cell growth factor in the example composition is significantly better than that of the comparative examples. That is, it can be seen that panthenol inhibits the reduction of the protein content, thereby increasing the stability of the protein composition.

Example 2 and Comparative Examples 6 to 8 Preparation of Cosmetic Composition

The cosmetic composition (emulsion) of Example 2 and Comparative Examples 6 to 8 was prepared in the same manner as in Example 1 and Comparative Examples 1 to 5 with the configuration of Table 3 below (unit: wt%). Example 1 includes polyglyceryl-3 methylglucose distearate, which is a polyalcohol ester compound, while Comparative Examples 6 to 8 do not include it.

TABLE 3

ingredient	Example 2	Comparative Example 6	Comparative Example 7	Comparative Example 8
Squalane	5	5	5	5
100 ppm solution of epidermal growth factor	One	One	One	One
Polyglyceryl-3 Methylglucose Distearate	2	-	-	-
Hydrogenated Lecithin / C 12-16 Alcohol / Palmite Acid (0.65: 0.175: 0.175)	-	2	-	-
C 14-22 Alcohol / C 12-20 Alkyl Glucoside (0.2: 0.8)	-	-	2	-
Sucrose fatty acid ester	-	-	-	2
Carbomer	2	2	2	2
Sodium hydroxide	0.2	0.2	0.2	0.2
Distilled water	89.8	89.8	89.8	89.8

Experimental Example 2 Evaluation of Protein Stabilization of Polyalcohol Ester Compound

In order to determine the stability of the epidermal growth factor contained in the composition of Examples 2 and Comparative Examples 8 to 8 above, the epidermal growth factor content of each composition was measured (initial value), and at 4 ° C. and 25 ° C. for 1 year. After storage, the content of epidermal growth factor was confirmed by ELISA. The results are shown in the table below.

Table 4

	Growth factor content (ppm)
	Initial value	1 year after cold storage	1 year after storage at 25 ℃
Example 2	0.1175	0.0184	0.0059
Comparative Example 6	0.1138	-0.0019	-0.0191
Comparative Example 7	0.0971	0.0091	0.0012
Comparative Example 8	0.1192	0.0091	-0.0019

As can be seen from the above results, it may be helpful to stabilize the hydrogenated series materials (hydrogenated lecithin / C _12-16 alcohol / palmitic acid) or sugar-based fatty acid esters that have been used as ingredients for formulating proteins. The epithelial cell growth factor content is higher after one year of the example composition than the composition of the comparative examples comprising the expected surfactant (C _14-22 alcohol / C _12-20 alkyl glucoside or sucrose fatty acid ester). This means that the polyalcohol ester compound can increase the stability of the protein, and particularly, the effect is better when refrigerated than when stored at room temperature. Through this, it can be seen that the polyalcohol ester compound has an effect of stabilizing the protein because it inhibits the reduction of the protein content for a long storage period.

[Examples 3 and 4 and Comparative Examples 9 to 14] Preparation of Cosmetic Composition

The cosmetic composition (emulsion) of Examples 3 and 4 and Comparative Examples 9 to 14 with the configuration of Table 5 below was prepared in substantially the same manner as Example 1 and Comparative Examples 1 to 5 (unit: wt%). Example 3 contains casein hydrolysates, while Example 4 contains casein sodium, whereas Comparative Examples 9-14 do not.

Table 5

ingredient	Example 3	Example 4	Comparative Example 9	Comparative Example 10	Comparative Example 11	Comparative Example 12	Comparative Example 13	Comparative Example 14
Squalane	5	5	5	5	5	5	5	5
100 ppm solution of epidermal growth factor	One	One	One	One	One	One	One	One
Casein Hydrolyzate 1%	One	-	-	-	-	-	-	-
Casein sodium 1%	-	One	-	-	-	-	-	-
Lactoferrin solution 1%	-	-	-	One	-	-	-	-
Gelatin 1%	-	-	-	-	One	-	-	-
Glycoprotein 1%	-	-	-	-	-	One	-	-
Whey 1%	-	-	-	-	-	-	One	-
L-Theanine 1%	-	-	-	-	-	-	-	One
Carbomer	2	2	2	2	2	2	2	2
Sodium hydroxide	0.2	0.2	0.2	0.2	0.2	0.2	0.2	0.2
Distilled water	90.8	90.8	91.8	90.8	90.8	90.8	90.8	90.8

Experimental Example 3 Evaluation of Protein Stability of Casein and Its Derivatives

In order to determine the stability of the epidermal growth factor contained in the composition of Examples 3 and 4 and Comparative Examples 9 to 14, the initial content of epithelial cell growth factor of each composition was confirmed by ELISA. After storage for 1 year at 4 ℃, 25 ℃ and 45 ℃ and then measured the epidermal growth factor content in the same way, and after calculating the relative thermal stability (%) compared with the initial content for each storage temperature It is shown in the table below.

Table 6

sample	4 ℃ Relative Thermal Stability (%)	25 ℃ relative thermal stability (%)	45 ℃ Relative Thermal Stability (%)
Example 3	81.9	57.0	18.2
Example 4	90.3	65.2	11.8
Comparative Example 9	45.4	38.8	11.5
Comparative Example 10	46.6	38.2	8.6
Comparative Example 11	45.2	35.1	18.5
Comparative Example 12	44.0	9.5	-0.3
Comparative Example 13	54.3	29.1	10.3
Comparative Example 14	44.1	36.0	10.9

As can be seen from the above results, the epithelial cell growth factor content of the composition of the Example composition is higher than that of the comparative examples comprising lactoferrin, gelatin, glycoprotein (glycoprotein), whey or L-theanine, which are known as conventional protein stabilizing substances. high. This means that casein and its derivatives can increase the stability of epidermal growth factor, and particularly, the effect is better when stored at a temperature below room temperature. Through this, it can be confirmed that casein and its derivatives have an effect of increasing the stability of the epidermal growth factor composition because it inhibits the decrease of the epidermal growth factor content for a long storage period.

Hereinafter, the formulation of the composition according to an aspect of the present invention will be described in more detail, but the composition is applicable to various formulations, which is intended to explain in detail only, not intended to limit the present invention.

Formulation Example 1 Flexible Lotion

According to the composition described in the table below to prepare a flexible lotion in a conventional manner.

TABLE 7

ingredient	Content (% by weight)
Panthenol	2.0
100 ppm solution of epidermal growth factor	5.0
glycerin	3.0
Butylene glycol	2.0
Propylene glycol	2.0
Carboxy Vinyl Polymer	0.1
Fiji-12 nonylphenyl ether	0.2
Polysorbate 80	0.4
ethanol	10.0
Triethanolamine	0.1
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 2 Nutritional Lotion (Milk Lotion)

To prepare a nourishing lotion according to the composition described in the table below.

Table 8

ingredient	Content (% by weight)
Polyglyceryl-3 Methylglucose Distearate	5.0
100 ppm solution of epidermal growth factor	5.0
glycerin	3.0
Butylene glycol	3.0
Propylene glycol	3.0
Carboxy Vinyl Polymer	0.1
Beeswax	4.0
Polysorbate 60	1.5
Caprylic / Capric Triglycerides	5.0
Squalane	5.0
Sorbitassquioleate	1.5
Liquid paraffin	0.5
Cetearyl Alcohol	1.0
Triethanolamine	0.2
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 3 Nutrition Cream

Nutritional creams are prepared by conventional methods according to the compositions set forth in the table below.

Table 9

ingredient	Content (% by weight)
Casein hydrolyzate	7.0
Epithelial Growth Factor Solution	7.0
glycerin	3.0
Butylene glycol	3.0
Liquid paraffin	7.0
Beta Glucan	7.0
Carbomer	0.1
Caprylic / Capric Triglycerides	3.0
Squalane	5.0
Cetearyl Glucoside	1.5
Sorbitan stearate	0.4
Polysorbate 60	1.2
Triethanolamine	0.1
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 4 Massage Cream

Massage cream is prepared in a conventional manner according to the composition described in the table below.

Table 10

ingredient	Content (% by weight)
Panthenol	8.0
100 ppm solution of epidermal growth factor	5.0
glycerin	8.0
Butylene glycol	4.0
Liquid paraffin	45.0
Beta Glucan	7.0
Carbomer	0.1
Caprylic / Capric Triglycerides	3.0
Beeswax	4.0
Cetearyl Glucoside	1.5
Sesqui oleic acid sorbitan	0.9
Vaseline	3.0
paraffin	1.5
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

[Formulation Example 5] Pack

Packs are prepared by conventional methods according to the compositions described in the table below.

Table 11

ingredient	Content (% by weight)
Polyglyceryl-3 Methylglucose Distearate	8.0
100 ppm solution of epidermal growth factor	8.0
glycerin	4.0
Polyvinyl alcohol	15.0
Hyaluronic acid extract	5.0
Beta Glucan	7.0
Allantoin	0.1
Nonyl Phenyl Ether	0.4
Polysorbate 60	1.2
ethanol	6.0
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Formulation Example 6 Injection

Injections are prepared by conventional methods according to the compositions listed in the table below.

Table 12

ingredient	Content (% by weight)
Casein sodium	0.5
100 ppm solution of epidermal growth factor	0.5
NaCl	0.4
Purified water	Remaining amount

Table 13

ingredient	Content (% by weight)
Panthenol	5
NaCl	0.4
albumin	1.0
Botulinum Toxin	100 units
Purified water	Remaining amount

Formulation Example 7 Ointment

Ointments are prepared by conventional methods according to the compositions described in the table below.

Table 14

ingredient	Content (% by weight)
Polyglyceryl-3 Methylglucose Distearate	0.1
glycerin	8.0
Butylene glycol	4.0
Floating paraffin	15.0
Beta Glucan	7.0
Carbomer	0.1
Caprylic / Capric Triglycerides	3.0
Squalane	1.0
Cetearyl Glucoside	1.5
Sorbitan stearate	0.4
Cetearyl Alcohol	1.0
Beeswax	4.0
Preservative, coloring, flavoring	Quantity
Purified water	Remaining amount

Claims (15)

1. Peptide or protein stabilizer comprising at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof.
2. The method of claim 1,

   The peptide or protein is a stabilizer comprising one or more selected from the group consisting of growth factors, hormones, interferons, vaccines, recombinant proteins, recombinant peptides and enzymes.
3. The method of claim 1,

   The polyalcohol ester compound or derivative thereof comprises at least one selected from the group consisting of polyglyceryl distearate, PEG distearate, polyglyceryl methylglucose distearate and PEG methylglucose distearate.
4. The method of claim 1,

   Casein or a derivative thereof is a stabilizer comprising at least one selected from the group consisting of casein hydrolyzate and casein salt.
5. Cosmetic composition comprising a stabilizer according to any one of claims 1 to 4.
6. A pharmaceutical composition comprising a stabilizer according to any one of claims 1 to 4.
7. One or more of peptides and proteins; And

   A composition comprising at least one selected from the group consisting of panthenol, polyalcohol ester compounds, casein, and derivatives thereof.
8. The method of claim 7, wherein

   At least one of the peptides and proteins comprises at least one selected from the group consisting of growth factors, hormones, interferons, vaccines, recombinant proteins, recombinant peptides and enzymes,

   The polyalcohol ester compound or derivative thereof includes at least one selected from the group consisting of polyglyceryl distearate, PEG distearate, polyglyceryl methylglucose distearate and PEG methylglucose distearate,

   Casein or a derivative thereof comprises at least one selected from the group consisting of casein hydrolyzate and casein salt.
9. The method of claim 7, wherein

   The composition comprises 0.1 to 50% by weight of at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof, based on the total weight of the composition.
10. The method of claim 7, wherein

    The composition has a viscosity of 5,000 cP to 30,000 cP.
11. The method according to any one of claims 7 to 10,

    A composition which is a cosmetic composition.
12. The method according to any one of claims 7 to 10,

    A composition that is a pharmaceutical composition.
13. One or more of peptides and proteins; And mixing at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein, and derivatives thereof to prepare a composition.
14. The method of claim 13,

    At least one of the peptides and proteins comprises at least one selected from the group consisting of growth factors, hormones, interferons, vaccines, recombinant proteins, recombinant peptides and enzymes,

    The polyalcohol ester compound or derivative thereof comprises at least one selected from the group consisting of polyglyceryl distearate, PEG distearate, polyglyceryl methylglucose distearate and PEG methylglucose distearate,

    Casein or a derivative thereof comprises at least one selected from the group consisting of casein hydrolysate and casein salt.
15. The method of claim 13,

    The step of preparing the composition is a method for preparing the composition by mixing at least one selected from the group consisting of panthenol, polyalcohol ester compound, casein and derivatives thereof in 0.1 to 50% by weight relative to the total weight of the composition.