WO2012067999A3 - Pathogen-derived effectors and their use in enhancing plant traits - Google Patents

Pathogen-derived effectors and their use in enhancing plant traits Download PDF

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Publication number
WO2012067999A3
WO2012067999A3 PCT/US2011/060567 US2011060567W WO2012067999A3 WO 2012067999 A3 WO2012067999 A3 WO 2012067999A3 US 2011060567 W US2011060567 W US 2011060567W WO 2012067999 A3 WO2012067999 A3 WO 2012067999A3
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WO
WIPO (PCT)
Prior art keywords
pathogen
derived
plants
effectors
enhancing plant
Prior art date
Application number
PCT/US2011/060567
Other languages
French (fr)
Other versions
WO2012067999A2 (en
Inventor
Nickolas J. Panopoulos
Antigoni Stylianou Papanikolaou
Panagiotis Sarris
Nickolas Skandalis
Original Assignee
Panopoulos Nickolas J
Antigoni Stylianou Papanikolaou
Panagiotis Sarris
Nickolas Skandalis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Panopoulos Nickolas J, Antigoni Stylianou Papanikolaou, Panagiotis Sarris, Nickolas Skandalis filed Critical Panopoulos Nickolas J
Publication of WO2012067999A2 publication Critical patent/WO2012067999A2/en
Publication of WO2012067999A3 publication Critical patent/WO2012067999A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Insects & Arthropods (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Method for producing transgenic plants that are resistant to at least one pathogen and one insect pest are provided. Plants expressing a pathogen-derived effector that are resistant to at least one pathogen, at least one insect pest, at freezing stress are provided. Genes and gene sequences comprising pathogen-derived effectors chosen as to not trigger the hypersensitive response and to be capable of enhancing gene silencing on a given plant are also provided, as well as plants, plant parts, plant cells, seeds, and non-human host cells expressing such genes.
PCT/US2011/060567 2010-11-15 2011-11-14 Pathogen-derived effectors and their use in enhancing plant resistance to pathogens, insect pests and freezing stress WO2012067999A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41373110P 2010-11-15 2010-11-15
US61/413,731 2010-11-15
US41514910P 2010-11-18 2010-11-18
US61/415,149 2010-11-18

Publications (2)

Publication Number Publication Date
WO2012067999A2 WO2012067999A2 (en) 2012-05-24
WO2012067999A3 true WO2012067999A3 (en) 2012-12-13

Family

ID=46084585

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/060567 WO2012067999A2 (en) 2010-11-15 2011-11-14 Pathogen-derived effectors and their use in enhancing plant resistance to pathogens, insect pests and freezing stress

Country Status (1)

Country Link
WO (1) WO2012067999A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020083489A1 (en) * 2000-04-03 2002-06-27 Alan Collmer DNA molecules and polypeptides of pseudomonas syringae Hrp pathogenicity island and their uses
US20070162994A1 (en) * 2002-02-12 2007-07-12 Alan Collmer Pseudomonas avr and hop proteins, their encoding nucleic acids, and use thereof
US20100257621A1 (en) * 2006-10-03 2010-10-07 Monsanto Technology Llc Methods for Hybrid Corn Seed Production and Compositions Produced Therefrom

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020083489A1 (en) * 2000-04-03 2002-06-27 Alan Collmer DNA molecules and polypeptides of pseudomonas syringae Hrp pathogenicity island and their uses
US20070162994A1 (en) * 2002-02-12 2007-07-12 Alan Collmer Pseudomonas avr and hop proteins, their encoding nucleic acids, and use thereof
US20100257621A1 (en) * 2006-10-03 2010-10-07 Monsanto Technology Llc Methods for Hybrid Corn Seed Production and Compositions Produced Therefrom

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ESCOBAR ET AL.: "RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis.", PROC NAT ACAD SCI, vol. 98, no. 23, 6 November 2001 (2001-11-06), pages 13437 - 13442 *
KATIYAR-AGARWAL ET AL.: "A pathogen-inducible endogenous siRNA in plant immunity.", PROC NAT ACAD SCI, vol. 103, no. 47, 21 November 2006 (2006-11-21), pages 18002 - 18007 *
LANDGRAF ET AL.: "Different versions of Pseudomonas syringae pv. tomato DC3000 exist due to the activity of an effector transposon.", MOL PLANT PATHOL SEPTEMBER, vol. 7, no. 5, 2006, pages 355 - 364 *
RUBENSTEIN ET AL.: "Long-term association of tomato yellow leaf curl virus with its whitefly vector Bemisia tabaci: effect on the insect transmission capacity, longevity and fecundity.", J GEN MICROBIOL, vol. 78, 1997, pages 2683 - 2689 *
SARRIS ET AL.: "Phytobacterial type III effectors HopXl, HopAB1 and HopF2 enhance sense- post-transcriptional gene silencing independently of plant R gene-effector recognition.", MOL PLANT MICROBE INTERACT, vol. 24, no. 8, August 2011 (2011-08-01), pages 907 - 917 *

Also Published As

Publication number Publication date
WO2012067999A2 (en) 2012-05-24

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