WO2012065075A3 - Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) - Google Patents

Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) Download PDF

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Publication number
WO2012065075A3
WO2012065075A3 PCT/US2011/060391 US2011060391W WO2012065075A3 WO 2012065075 A3 WO2012065075 A3 WO 2012065075A3 US 2011060391 W US2011060391 W US 2011060391W WO 2012065075 A3 WO2012065075 A3 WO 2012065075A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
dep
devices
dielectrophoresis
high voltage
Prior art date
Application number
PCT/US2011/060391
Other languages
French (fr)
Other versions
WO2012065075A2 (en
Inventor
Michael Heller
Avery Sonnenberg
Rajaram Krishnan
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Publication of WO2012065075A2 publication Critical patent/WO2012065075A2/en
Publication of WO2012065075A3 publication Critical patent/WO2012065075A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/024—Non-uniform field separators using high-gradient differential dielectric separation, i.e. using a dielectric matrix polarised by an external field
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Devices and techniques are disclosed that provide single and multi "pore-based" AC/DC high voltage electrokinetic devices and improved features and methods for carrying out dielectrophoresis (DEP) under high conductance conditions. The electrokinetic devices and methods allow rapid isolation, separation, detection, and analysis of cells, bacteria, virus, cell free circulating (cfc) DNA, cfc-RNA, and other cellular nanoparticulates (exosomes, etc.), and also allow drug delivery and separation of other nanoparticles to be carried out under high conductance conditions directly from blood, plasma, serum, urine, and other clinical, biological, and environmental samples in an unaltered condition.
PCT/US2011/060391 2010-11-12 2011-11-11 Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) WO2012065075A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41330610P 2010-11-12 2010-11-12
US61/413,306 2010-11-12

Publications (2)

Publication Number Publication Date
WO2012065075A2 WO2012065075A2 (en) 2012-05-18
WO2012065075A3 true WO2012065075A3 (en) 2012-10-04

Family

ID=46051589

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/060391 WO2012065075A2 (en) 2010-11-12 2011-11-11 Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep)

Country Status (1)

Country Link
WO (1) WO2012065075A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150184225A1 (en) * 2012-06-25 2015-07-02 The Arizona Board Of Regents On Behalf Of The University Of Arizona Electrokinetic polymerase chain reaction (pcr) devices and methods
CN105241940B (en) * 2015-08-05 2018-04-27 深圳大学 A kind of Composition analyzed method and its system based on the dielectrophoresis field of force

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280590B1 (en) * 1996-09-06 2001-08-28 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US20060201811A1 (en) * 2005-03-04 2006-09-14 Hamers Robert J Apparatus for transport and analysis of particles using dielectrophoresis
WO2009146143A2 (en) * 2008-04-03 2009-12-03 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
US7658829B2 (en) * 2005-04-08 2010-02-09 Uti Limited Partnership Integrated microfluidic transport and sorting system
US7666289B2 (en) * 2005-03-11 2010-02-23 Sandia Corporation Methods and devices for high-throughput dielectrophoretic concentration

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280590B1 (en) * 1996-09-06 2001-08-28 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US20060201811A1 (en) * 2005-03-04 2006-09-14 Hamers Robert J Apparatus for transport and analysis of particles using dielectrophoresis
US7666289B2 (en) * 2005-03-11 2010-02-23 Sandia Corporation Methods and devices for high-throughput dielectrophoretic concentration
US7658829B2 (en) * 2005-04-08 2010-02-09 Uti Limited Partnership Integrated microfluidic transport and sorting system
WO2009146143A2 (en) * 2008-04-03 2009-12-03 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers

Also Published As

Publication number Publication date
WO2012065075A2 (en) 2012-05-18

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