WO2012048334A3 - Novel fungal proteases - Google Patents

Novel fungal proteases Download PDF

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Publication number
WO2012048334A3
WO2012048334A3 PCT/US2011/055703 US2011055703W WO2012048334A3 WO 2012048334 A3 WO2012048334 A3 WO 2012048334A3 US 2011055703 W US2011055703 W US 2011055703W WO 2012048334 A3 WO2012048334 A3 WO 2012048334A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
novel
relates
methods
processing
Prior art date
Application number
PCT/US2011/055703
Other languages
French (fr)
Other versions
WO2012048334A2 (en
Inventor
Johannes Visser
Sandra Hinz
Jan Werij
Jacob Visser
Vivi Joosten
Martijn Koetsier
Mark Emalfarb
Original Assignee
Dyadic International (Usa) Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dyadic International (Usa) Inc. filed Critical Dyadic International (Usa) Inc.
Publication of WO2012048334A2 publication Critical patent/WO2012048334A2/en
Publication of WO2012048334A3 publication Critical patent/WO2012048334A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0326—Rennet produced by fermentation, e.g. microbial rennet; Rennet produced by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to the degradation of protein and novel combinations of enzymes, including those that provide a synergistic release of amino acids or lower molecular weight proteins. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing or treating of clothing or fabrics, detergent processes, cleaning of carpets, leather treatment, animal feed, food processing, baking, increasing the protein yield of food, modification of food taste, processing of proteins in protein production, processing of beverages, medical treatment, and cosmetic treatment, and bio fuels.
PCT/US2011/055703 2010-10-08 2011-10-11 Novel fungal proteases WO2012048334A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39156510P 2010-10-08 2010-10-08
US61/391,565 2010-10-08

Publications (2)

Publication Number Publication Date
WO2012048334A2 WO2012048334A2 (en) 2012-04-12
WO2012048334A3 true WO2012048334A3 (en) 2013-01-10

Family

ID=45928495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/055703 WO2012048334A2 (en) 2010-10-08 2011-10-11 Novel fungal proteases

Country Status (1)

Country Link
WO (1) WO2012048334A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2760997A4 (en) 2011-09-30 2015-02-11 Codexis Inc Fungal proteases
RU2645252C2 (en) 2012-01-05 2018-02-19 Глюкос Финланд Ой Felamentous fungi cell with protease deficit and methods of its application
CN104884615B (en) 2012-09-05 2019-07-12 诺维信公司 Polypeptide with proteinase activity
EP2712915A1 (en) * 2012-10-01 2014-04-02 The Procter and Gamble Company Methods of treating a surface and compositions for use therein
EP2735303A1 (en) 2012-11-23 2014-05-28 Pilosciences Hair growth compositions and methods
RU2016104064A (en) 2013-07-10 2017-08-15 Новартис Аг CELLS OF MYCELIUM MUSHROOMS WITH MULTIPLE INSUFFICIENCY OF PROTEASES AND METHODS OF USING THEM
DE102013226729A1 (en) * 2013-12-19 2015-06-25 Henkel Ag & Co. Kgaa Fungal proteases and their use in detergents and cleaners
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
BR112018010310A2 (en) 2015-12-02 2018-12-04 Basf Se recombinant polypeptide production method, filamentous fungus, use of nucleic acid construction and use of filamentous fungus
BR112018009729A8 (en) 2015-12-02 2019-02-26 Basf Se method for producing a recombinant polypeptide, filamentous fungus myceliophthora thermophila and use of a nucleic acid construct
BR112019025950A2 (en) 2017-06-09 2020-09-15 Novozymes A/S polypeptide, use and method to hydrolyze protein
TW201904653A (en) 2017-06-30 2019-02-01 美商泰華施公司 Membrane cleaning solution and method for using the same for accelerating film cleaning
AU2020228355A1 (en) * 2019-02-25 2021-09-16 International N&H Denmark Aps Protein hydrolysates with increased yield of N-terminal amino acid
WO2023148301A1 (en) 2022-02-02 2023-08-10 Biotalys NV Modified microbial cells and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013452A (en) * 1995-12-15 2000-01-11 Novo Nordisk A/S Fungus wherein the areA, pepC and/or pepE genes have been inactivated
US6309868B1 (en) * 1997-07-05 2001-10-30 Nestec S.A. Cloning of the prolyl-dipeptidyl-peptidase from Aspergillus oryzae
US20060246545A1 (en) * 2005-04-12 2006-11-02 Huaming Wang Gene inactivated mutants with altered protein production
US20090099079A1 (en) * 2007-09-07 2009-04-16 Emalfarb Mark A Novel Fungal Enzymes
US20090253173A1 (en) * 2008-04-08 2009-10-08 Danisco Us Inc., Genencor Division Filamentous fungi with inactivated protease genes for altered protein production

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013452A (en) * 1995-12-15 2000-01-11 Novo Nordisk A/S Fungus wherein the areA, pepC and/or pepE genes have been inactivated
US6309868B1 (en) * 1997-07-05 2001-10-30 Nestec S.A. Cloning of the prolyl-dipeptidyl-peptidase from Aspergillus oryzae
US20060246545A1 (en) * 2005-04-12 2006-11-02 Huaming Wang Gene inactivated mutants with altered protein production
US20090099079A1 (en) * 2007-09-07 2009-04-16 Emalfarb Mark A Novel Fungal Enzymes
US20090253173A1 (en) * 2008-04-08 2009-10-08 Danisco Us Inc., Genencor Division Filamentous fungi with inactivated protease genes for altered protein production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BERKA, R. M. ET AL.: "Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris", NATURE BIOTECHNOLOGY, vol. 29, no. 10, 2 October 2011 (2011-10-02), pages 922 - 929, XP055034838, DOI: doi:10.1038/nbt.1976 *

Also Published As

Publication number Publication date
WO2012048334A2 (en) 2012-04-12

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