WO2012047324A2 - Systèmes et procédés pour amplification et présentation de phage - Google Patents

Systèmes et procédés pour amplification et présentation de phage Download PDF

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Publication number
WO2012047324A2
WO2012047324A2 PCT/US2011/039932 US2011039932W WO2012047324A2 WO 2012047324 A2 WO2012047324 A2 WO 2012047324A2 US 2011039932 W US2011039932 W US 2011039932W WO 2012047324 A2 WO2012047324 A2 WO 2012047324A2
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WO
WIPO (PCT)
Prior art keywords
library
clones
droplets
phage
distinguishable
Prior art date
Application number
PCT/US2011/039932
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English (en)
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WO2012047324A3 (fr
Inventor
Ratmir Derda
Sindy K.Y. Tang
George M. Whitesides
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President And Fellows Of Harvard College
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Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Priority to US13/702,603 priority Critical patent/US9499813B2/en
Publication of WO2012047324A2 publication Critical patent/WO2012047324A2/fr
Publication of WO2012047324A3 publication Critical patent/WO2012047324A3/fr
Priority to US15/294,208 priority patent/US20170298342A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne généralement l'amplification d'entités biologiques, par exemple, pour présentation de phage. Dans un aspect, des membres d'une banque d'entités biologiques sont encapsulés dans des compartiments séparés (par exemple, dans des gouttelettes microfluidiques séparées) et amplifiés. En tant qu'exemple spécifique, en plaçant des membres d'une banque de présentation sur phage dans des gouttelettes microfluidiques de sorte qu'aucune gouttelette ne contienne plus d'un membre de la banque, la banque peut être amplifiée sans aucun changement substantiel des taux de croissance ou des distributions de population, ou d'autres artefacts créés en raison de différences de taux de croissance ou d'amplification entre différents membres de la banque. Dans certains cas, le volume des compartiments peut être utilisé pour contrôler le nombre de copies d'une entité biologique pendant l'amplification. Dans certains cas, des entités biologiques avec différents taux d'amplification peuvent être amplifiées indépendamment les unes des autres. Dans certains modes de réalisation, le rapport entre une entité biologique à amplification rapide et une entité biologique à amplification lente peut être modulé. Cela peut être avantageux, par exemple, dans la conservation de la diversité au sein d'une banque en empêchant des entités biologiques à amplification rapide de dominer des entités biologiques à amplification lentes. Par exemple, certains procédés et systèmes de l'invention peuvent être utiles dans des situations dans lesquelles l'amplification préférentielle de membres d'une banque peut poser un problème.
PCT/US2011/039932 2010-06-10 2011-06-10 Systèmes et procédés pour amplification et présentation de phage WO2012047324A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/702,603 US9499813B2 (en) 2010-06-10 2011-06-10 Systems and methods for amplification and phage display
US15/294,208 US20170298342A1 (en) 2010-06-10 2016-10-14 Systems and methods for amplification and phage display

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35332410P 2010-06-10 2010-06-10
US61/353,324 2010-06-10

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/702,603 A-371-Of-International US9499813B2 (en) 2010-06-10 2011-06-10 Systems and methods for amplification and phage display
US15/294,208 Continuation US20170298342A1 (en) 2010-06-10 2016-10-14 Systems and methods for amplification and phage display

Publications (2)

Publication Number Publication Date
WO2012047324A2 true WO2012047324A2 (fr) 2012-04-12
WO2012047324A3 WO2012047324A3 (fr) 2012-09-07

Family

ID=45567099

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/039932 WO2012047324A2 (fr) 2010-06-10 2011-06-10 Systèmes et procédés pour amplification et présentation de phage

Country Status (2)

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US (2) US9499813B2 (fr)
WO (1) WO2012047324A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015048391A1 (fr) 2013-09-27 2015-04-02 The Board Of Trustees Of The University Of Illinois Procédé et nécessaire permettant de générer des agents de liaison à haute affinité
EP2878374B1 (fr) * 2013-11-29 2021-05-12 IMEC vzw Procédé pour effectuer une PCR numérique
US10928382B2 (en) * 2014-06-26 2021-02-23 Northeastern University Microfluidic device and method for analysis of tumor cell microenvironments
EP3524352A1 (fr) * 2014-10-24 2019-08-14 The Board of Trustees of the Leland Stanford Junior University Émulsion fluorée de pickering
US11039789B2 (en) 2015-11-23 2021-06-22 Verily Life Sciences Llc In-vivo magnetic detection of magnetic nanoparticles using microneedles
ES2841907T3 (es) * 2019-02-08 2021-07-12 Lightcast Discovery Ltd Método de manipulación de microgotitas

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Also Published As

Publication number Publication date
WO2012047324A3 (fr) 2012-09-07
US9499813B2 (en) 2016-11-22
US20170298342A1 (en) 2017-10-19
US20130210680A1 (en) 2013-08-15

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