WO2012041761A3 - Botulinum neurotoxin polypeptides exhibiting a prolonged activity - Google Patents

Botulinum neurotoxin polypeptides exhibiting a prolonged activity Download PDF

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Publication number
WO2012041761A3
WO2012041761A3 PCT/EP2011/066504 EP2011066504W WO2012041761A3 WO 2012041761 A3 WO2012041761 A3 WO 2012041761A3 EP 2011066504 W EP2011066504 W EP 2011066504W WO 2012041761 A3 WO2012041761 A3 WO 2012041761A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
neurotoxin
botulinum neurotoxin
neurotoxin polypeptide
polypeptides exhibiting
Prior art date
Application number
PCT/EP2011/066504
Other languages
French (fr)
Other versions
WO2012041761A2 (en
Inventor
Juergen Frevert
Original Assignee
Merz Pharma Gmbh & Co. Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merz Pharma Gmbh & Co. Kgaa filed Critical Merz Pharma Gmbh & Co. Kgaa
Publication of WO2012041761A2 publication Critical patent/WO2012041761A2/en
Publication of WO2012041761A3 publication Critical patent/WO2012041761A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A61K38/4893—Botulinum neurotoxin (3.4.24.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Cosmetics (AREA)

Abstract

The present invention pertains to the field of modified neurotoxin polypeptides. In particular, it relates to a polynucleotide encoding a neurotoxin polypeptide comprising at least two neurotoxin light chains and a heavy chain. The neurotoxin polypeptide encoded by the polynucleotide exhibits, in an aspect, a prolonged duration of the biological effect in a subject. Moreover, contemplated are a vector and a host cell which comprise the polynucleotide as well as a neurotoxin polypeptide encoded by the aforementioned polynucleotide. Further, the present invention relates to a medicament comprising the polynucleotide, the vector or the neurotoxin polypeptide and its therapeutic and cosmetic applications.
PCT/EP2011/066504 2010-09-28 2011-09-22 Botulinum neurotoxin polypeptides exhibiting a prolonged activity WO2012041761A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40416610P 2010-09-28 2010-09-28
EP10181374 2010-09-28
EP10181374.9 2010-09-28
US61/404,166 2010-09-28

Publications (2)

Publication Number Publication Date
WO2012041761A2 WO2012041761A2 (en) 2012-04-05
WO2012041761A3 true WO2012041761A3 (en) 2012-08-16

Family

ID=43302480

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/066504 WO2012041761A2 (en) 2010-09-28 2011-09-22 Botulinum neurotoxin polypeptides exhibiting a prolonged activity

Country Status (1)

Country Link
WO (1) WO2012041761A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10792344B2 (en) * 2006-06-29 2020-10-06 Merz Pharma Gmbh & Co. Kgaa High frequency application of botulinum toxin therapy
FI3481852T3 (en) * 2016-07-08 2023-03-19 Childrens Medical Center A novel botulinum neurotoxin and its derivatives
EP3641746A4 (en) * 2017-06-19 2021-03-17 President And Fellows Of Harvard College Methods and compositions for treating a microbial infection

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010022979A1 (en) * 2008-08-29 2010-03-04 Merz Pharma Gmbh & Co. Kgaa Clostridial neurotoxins with altered persistency

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010022979A1 (en) * 2008-08-29 2010-03-04 Merz Pharma Gmbh & Co. Kgaa Clostridial neurotoxins with altered persistency

Also Published As

Publication number Publication date
WO2012041761A2 (en) 2012-04-05

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