WO2012032802A1 - Appareil pour la concentration de particules et appareil pour la concentration et l'extraction de particules - Google Patents

Appareil pour la concentration de particules et appareil pour la concentration et l'extraction de particules Download PDF

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Publication number
WO2012032802A1
WO2012032802A1 PCT/JP2011/055136 JP2011055136W WO2012032802A1 WO 2012032802 A1 WO2012032802 A1 WO 2012032802A1 JP 2011055136 W JP2011055136 W JP 2011055136W WO 2012032802 A1 WO2012032802 A1 WO 2012032802A1
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particle
electrode
flow path
concentrating
particles
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PCT/JP2011/055136
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English (en)
Japanese (ja)
Inventor
昌廣 元祐
眞治 本阿弥
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学校法人東京理科大学
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Publication of WO2012032802A1 publication Critical patent/WO2012032802A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids

Definitions

  • the present invention relates to a particle concentrating device capable of concentrating fine particles contained in a liquid and a particle concentrating / extracting device capable of concentrating and extracting particles.
  • fine particles can be concentrated by controlling the difference in the electrophoresis speed of the target substance in solutions having different conductivity and pH.
  • an electroosmotic flow generation electrode is installed in the flow path separately from the electrophoresis electrode, and a predetermined DC voltage is applied to control the electrophoretic velocity and osmotic flow velocity of the charged particles, thereby concentrating. An effect can be obtained.
  • the fine particle As a method of switching a fine particle contained in a liquid to one selected from a plurality of alternative destinations physically and spatially, the fine particle is moved in a microchannel (i) an upstream location. And (ii) suspended in a fluid flowing through the branch junction to (iii) each of a plurality of branch channels leading to an alternative downstream destination, wherein the particles are a plurality of alternatives.
  • the particles suspended in the flowing fluid travel straight through the junction and flow to the first downstream destination.
  • the particles suspended in the flowing fluid travel straight through the junction and flow to the first downstream destination.
  • it deflects under the radiation beam and enters an alternative second downstream destination.
  • the cells or the population of particles are irradiated with light to physically organize the cells or the population of particles. Moving the light beam relative to the population of cells or particles, and the cells or particles physically organized at a rate that effectively separates at least a portion of the physically linear population of cells or particles.
  • a method of separating cells or particles using a moving beam of light including the step of moving a light beam with respect to a group (Japanese Patent Publication No. 2005-515071).
  • the population of cells or particles is physically bundled linearly, for example, by moving light rays at a constant speed with respect to the population of cells or particles.
  • a liquid transport force is applied to the solution in the flow path on the straight line from a direction opposite to the light irradiation direction, and the light radiation pressure and the liquid transport force are balanced, whereby the fine particles are irradiated with light. Sort along the direction.
  • Non-Patent Document 1 and Patent Document 1 concentrate particles contained in a liquid flow using electrophoresis and electroosmotic flow using a DC voltage.
  • There are disadvantages such as limited state and the like, and inapplicable to neutral fine particles, complicated introduction of a plurality of solutions, and high voltage of several hundred volts.
  • grains with a concentrated state is not realizable.
  • Patent Documents 2 to 4 all relate to a technique for separating particles by irradiating light contained in a liquid, but the development of a technique with higher separation efficiency has been made. It was desired.
  • the present invention has been made in view of the prior art, and an object of the present invention is to provide a technique that can concentrate and extract fine particles contained in a liquid with high efficiency and without depending on the charged state of the particles.
  • the particle concentrating device of the present invention is a particle concentrating device for concentrating particles in a liquid containing fine particles, wherein a main body in which a liquid channel including the fine particles is formed, and along the channel
  • a particle concentrating means having at least one pair of arranged electrodes and an AC power source for applying an AC voltage to the electrodes, and increasing the concentration of fine particles flowing in the flow path by the AC electroosmotic flow in the vicinity of the electrodes; It is characterized by providing.
  • an AC voltage is applied to at least a pair of electrodes to generate an AC electroosmotic flow due to an AC electric field in the gap space between the electrodes.
  • the particles in the liquid can be efficiently accumulated on the electrode.
  • stored on the electrode are conveyed toward a particle
  • the present invention also provides a particle concentration / extraction device for concentrating and extracting a particle concentration in a liquid containing fine particles, the main body having a liquid flow path including the fine particles, and the flow Having at least one pair of electrodes arranged along a path, and a first AC power source for applying an AC voltage to the electrodes, the concentration of fine particles flowing in the flow path by AC electroosmotic flow in the vicinity of the electrodes And at least two branch channels formed downstream of the particle concentration unit in the channel, wherein at least one of the branch channels is concentrated by the particle concentration unit.
  • a branch flow channel communicating with the concentrated particle take-out port for extracting the fine particles, and a flow of fine particles concentrated by the particle concentrating means are shifted to the branch flow channel communicating with the concentrated particle take-out port. It is characterized in that it comprises a and a particle extractor having a guiding means for.
  • an AC voltage is applied to at least a pair of electrodes to generate an AC electroosmotic flow due to an AC electric field in the gap space between the electrodes. Can be efficiently accumulated on the electrode. Further, since the particle concentrating / extracting device includes a particle extracting unit, the concentrated fine particles are extracted separately from the remaining liquid. Therefore, the operator's separation and extraction operation can be omitted.
  • the guiding means in the particle extraction unit is located upstream of the branch flow channel in the flow channel formed in the main body, and the concentrated particle collection from a branch flow channel not communicating with the concentrated particle extraction port.
  • the light emitting means includes a light emitting element composed of an LED element or a laser diode element, and a light emitting element driving power source for supplying power to the light emitting element.
  • the power source of the first AC power source that supplies power to the electrodes in the particle concentrating means and the power source of the light emitting element driving power source that supplies power to the light emitting elements in the light emitting means may be provided as a common power source. This simplifies the configuration of the power supply.
  • Connectors may be provided between the electrode and the first AC power source and between the light emitting element and the light emitting element driving power source, respectively. Thereby, the part except the power supply of a particle concentration / extraction apparatus can be made into a disposable type.
  • the induction means is arranged so as to be shifted laterally in a direction orthogonal to the flow direction from the position of the electrode of the particle concentration means, and an alternating voltage is applied to the induction electrode.
  • a second AC power source for applying the voltage may be included.
  • the second AC power supply may apply the same AC voltage to the induction electrode as the AC voltage applied to the electrode from the first AC power supply.
  • an apparatus that can concentrate and extract fine particles contained in a liquid with high efficiency is provided.
  • FIG. 1 is a schematic diagram illustrating the configuration of the particle concentration / extraction apparatus according to the first embodiment.
  • FIG. 2 is a cross-sectional view of the schematic diagram of the particle concentrating / extracting apparatus shown in FIG. 1 cut along a vertical plane along the longitudinal direction.
  • FIG. 3 is a plan view illustrating a configuration example of an electrode used in the particle concentrating unit of the particle concentrating / extracting apparatus according to the first embodiment.
  • FIG. 4 is an explanatory diagram showing that an AC electroosmotic flow is generated in the vicinity of an electrode to which an AC voltage is applied in the particle concentrating unit according to the first embodiment.
  • FIG. 5 is a diagram illustrating a modification of the particle extraction unit according to the first embodiment.
  • FIG. 1 is a schematic diagram illustrating the configuration of the particle concentration / extraction apparatus according to the first embodiment.
  • FIG. 2 is a cross-sectional view of the schematic diagram of the particle concentrating / extracting apparatus shown in FIG. 1 cut along a vertical plane along the longitudinal direction
  • FIG. 6 is a cross-sectional view showing the internal configuration of the particle concentration / extraction apparatus according to Embodiment 2 of the present invention.
  • FIG. 7 is a schematic diagram showing the electrode arrangement of the particle concentrating section and the particle guiding section in the particle concentrating / extracting apparatus shown in FIG.
  • FIG. 8 is a plan view showing the configuration of the particle concentration / extraction apparatus according to the third embodiment.
  • FIG. 9 is a cross-sectional view showing the XX cross section in FIG. 8 of the particle concentrating / extracting apparatus of the third embodiment.
  • FIG. 10 is a cross-sectional view illustrating another example of the particle concentration / extraction apparatus according to the third embodiment.
  • FIG. 11 is a plan view showing the configuration of the particle concentration / extraction apparatus according to the fourth embodiment.
  • FIG. 11 is a plan view showing the configuration of the particle concentration / extraction apparatus according to the fourth embodiment.
  • FIG. 12 is an explanatory diagram illustrating a particle distribution state on the electrode when no alternating current is applied to the electrode in the particle concentration / extraction apparatus according to the third embodiment.
  • FIG. 13 is an explanatory view showing a state where particles are collected on the central electrode when an alternating current is applied to the electrode in the particle concentration / extraction apparatus according to the third embodiment.
  • Embodiment 1 Hereinafter, an example of the particle concentration / extraction apparatus of the present invention will be described.
  • the present invention can be selectively configured as a particle concentrating device or a particle extracting device.
  • a particle concentrating / extracting device having both a particle concentrating function and an extracting function is provided. This will be described as an example.
  • the particle concentration / extraction apparatus 100 includes a plate-like main body 1 formed of a transparent material, a flow path 2 formed inside the main body 1, and A particle concentrating unit 3 disposed in the middle of the flow channel 2 and a particle extracting unit 4 provided in the vicinity of the downstream flow channel of the particle concentrating unit 3 are provided.
  • the particle concentration / extraction apparatus 100 of the present invention is configured in a microchip shape, and the main body 1 forms the main body of the microchip. Quartz, various glasses, polymethyl methacrylate resin, poly-4-methyl It is made of a transparent resin such as pentene-1 resin.
  • the main body 1 is preferably formed in a rectangular parallelepiped shape, and the flow path 2 is formed in an intermediate portion in the thickness direction.
  • the reason for using a transparent material for the main body 1 is to perform light irradiation to separate the concentrated particles from the remaining liquid. Therefore, in the embodiment in which the concentrated particles are separated without light irradiation, there is no reason why the main body must be made of a transparent material.
  • a liquid inlet 5 is provided at one end of the main body 1 connected to the flow path 2, and a concentrated fine particle solution is connected to the flow path at the end opposite to the inlet 5 of the main body 1.
  • a liquid outlet 7 which is an outlet for the remaining liquid from which the concentrated fine particle solution has been separated.
  • the flow path 2 is branched into two parts 43A and 43B, the branch flow path 43A is connected to the particle outlet 6 and the branch path 43B is connected to the liquid outlet 7.
  • the flow path 2 is branched in the thickness direction of the main body.
  • the flow path 2 is not particularly limited thereto, and may be branched according to the configuration of the particle extraction unit 4.
  • the particle concentrating unit 3 includes at least a pair of electrodes 31 disposed along the flow path 2 and a power source unit 33 that applies an AC voltage to the electrodes 31. It is desirable to connect the electrode 31 and the power supply unit 33 via a connector (not shown).
  • the power supply unit 33 can be configured by a DC power source, for example, a battery 33A, and a DC / AC conversion circuit 33B that converts a DC voltage supplied by the battery into an AC voltage having a predetermined voltage and frequency.
  • FIG. 3 shows a typical arrangement of electrodes in the present embodiment.
  • the electrodes 31 are arranged so that a pair of electrodes correspond to the upstream and downstream directions of the flow, but in an actual apparatus, as shown in FIG.
  • At least a pair of strip-like or plate-like electrodes extending in parallel to the direction, that is, electrodes 31A and 31B are arranged.
  • the pair of electrodes 31A and 31B are arranged with an interval of d.
  • the distance d is preferably 1 to 100 ⁇ m, more preferably 10 to 100 ⁇ m, and particularly preferably 20 to 100 ⁇ m from the viewpoint of ease of manufacture and the magnitude of the AC electroosmosis effect.
  • the length of the electrodes 31A and 31B in the direction of flow a is 1 mm or more, particularly 2 to 10 mm, the length is sufficient for the fine particle concentration positions to converge on the upper surfaces of the electrodes 31A and 31B. preferable.
  • the AC voltage applied to the electrodes 31A and 31B is preferably 5 V or less, particularly preferably in the range of 1 to 5 V, and the frequency of the AC voltage is preferably in the range of 0.1 to 10 kHz, particularly 1 to 5 kHz. A range is preferred.
  • the present invention concentrates fine particles in the solution using alternating current electroosmotic flow
  • the height of the flow path 2 that is, between the flow path side surface of the electrode and the upper surface of the flow path.
  • the distance is desirably set according to the concentration efficiency of the particles, and specifically, 100 ⁇ m or less is preferable.
  • the particle extraction unit 4 is provided on the downstream side of the particle concentration unit 3 and separates and extracts the high concentration fine particle solution concentrated in the liquid flow from the remaining liquid.
  • the particle extraction unit 4 irradiates light to the flow path 2 from the light emitting element 41 by applying a DC voltage to the light emitting element 41 provided to the main body 1 with the light irradiation direction directed to the flow path.
  • the light-emitting element drive circuit 42 includes, for example, an AC / DC conversion circuit for DC-converting the output of the DC / AC conversion circuit, branch flow paths 43A and 43B, a particle outlet 6 and a liquid outlet 7.
  • the light emitting element 41 and the light emitting element driving circuit 42 constitute a light emitting means which is an example of a guiding means in the particle concentration / extraction apparatus of the present invention.
  • the output light ⁇ of the light emitting element 41 is preferably visible light having a wavelength of 500 to 700 nm or near infrared light having a wavelength of about 1 ⁇ m.
  • the light output can be appropriately selected depending on how fast the particles are moved and depending on the object.
  • a light emitting element for outputting such light ⁇ an LED element, a laser diode element, or the like can be used.
  • a method of attaching the light emitting element 41 a method of installing the light emitting portion in the main body 1 or a method of installing the light emitting element 41 on the main body 1 using a substrate is possible.
  • the parts other than the power source unit can be made disposable. it can.
  • the particle concentrating / extracting device can be a portable device.
  • a solution containing fine particles is injected from the injection port 5 of the main body 1 and flows into the flow path 2 along the direction of the arrow a.
  • an AC voltage is applied to the electrodes 31A and 31B
  • an AC electric field is applied to the gap d between the electrodes 31A and 31B.
  • a flow alternating current electroosmotic flow
  • the fine particles are formed on the electrodes 31A and 31B. Accumulate.
  • FIG. 4 is a diagram for explaining the alternating current electroosmotic flow in the space above the electrode.
  • a flow AC electroosmotic flow b
  • fine particles in the electrode surface direction (downward in the figure) occurs in the space above the gap d between the electrodes 31A and 31B.
  • This flow flows separately in the vicinity of the electrode surface into flows along the two electrodes.
  • the speed of the flow along each electrode surface is the fastest in the vicinity of the gap between the electrodes and slows toward the outside, and the fine particles stay in the direction perpendicular to the flow near the outside of the center of the electrode. . That is, the fine particles flow in the flow direction a while staying in the direction perpendicular to the flow.
  • a flow indicated by a small arrow in the space above the electrodes 31A and 31B in FIG. 4 is a flow c caused by a flow b generated on the electrodes.
  • the fine particles flowing in the flow path 2 are collected in the vicinity of the electrode surface by the flow b and the flow c, and flow along the flow direction a.
  • the particles 10 accumulated on the electrodes 31A and 31B in the particle concentrating unit 3 move toward the particle extracting unit 4 on the liquid flow a.
  • the concentrated fine particles that have reached the particle extraction unit 4 are exposed to the irradiation light ⁇ emitted from the light emitting element 41.
  • the concentrated fine particles receive a downward pressing force (light pressure) by light as shown in FIG. 5 and flow in the direction of the branch flow path 43A, and the remaining branch flow path 43B has the remaining flow.
  • the liquid will flow.
  • the branch flow path 43 The concentrated fine particle solution that has flowed through A is taken out from the particle outlet 6, and the remaining liquid is taken out from the liquid outlet 7.
  • the particle concentrating / extracting apparatus 100 uses alternating current dielectrophoresis for concentrating particles in a liquid by using a force acting on the particles due to the mutual relationship between the liquid and the electrical properties (degree of polarization) of the particles.
  • the advantages over the particle concentration / extraction device are as follows.
  • the liquid force exerted on the particles is proportional to the square of the intensity of the electric field, but the liquid force exerts on the particles as the liquid moves on the electrodes 31A and 31B. To be moved. And the liquid force is only proportional to the particle size of the particles.
  • a particle having a diameter of 10 ⁇ m and a particle having a diameter of 100 nm differ only in the force exerted on the particle by a factor of 100. Therefore, the particle concentration / extraction apparatus 100 according to the first embodiment is much easier to apply to fine particles such as nano-sized particles than the particle concentration / extraction apparatus using alternating current dielectrophoresis.
  • the dielectrophoretic force is proportional to the square of the intensity of the electric field as described above, it rapidly attenuates as the distance from the electrode gap increases. Therefore, when trying to concentrate a wide range of particles in a particle concentration / extraction apparatus using alternating current dielectrophoresis, it is necessary to apply a high voltage to the electrode to increase the electric field strength.
  • the particles move in association with the liquid flow formed by the movement of the liquid on the electrodes 31A and 31B. Particles can be concentrated and extracted.
  • particles having a diameter of about 10 nm to 10 ⁇ m contained in the liquid can be collected in a non-contact manner, concentrated, and taken out from the particle outlet 6. Therefore, red blood cells, white blood cells, platelets, target cells, DNA, etc. in samples such as blood, cell suspensions, DNA suspensions, etc. can be moved or concentrated in place without mechanical damage. can do. Therefore, it can be used as a pre-processing / post-processing filter in various examinations or to enhance diagnosis / analysis sensitivity.
  • the antigen-antibody reaction can be promoted by concentrating the antigen to be measured in the particle concentrating / extracting apparatus 100, it is possible to measure at a very low concentration. High sensitivity of electrochemical sensor, pesticide residue detection and immunoassay can be achieved.
  • FIG. 5 shows an example in which a particle outlet 6A for taking out the concentrated particles of the particle extraction unit in the light irradiation direction of the light emitting element is provided.
  • the flow path in the main body 1 only forms the flow path 2 along the thickness of the main body, and the extraction flow path only needs to form the extraction flow path orthogonal to the flow path 2.
  • the said embodiment is comprised so that it may have two functions of particle concentration and isolation
  • Embodiment 2 Hereinafter, another example of the particle concentration / extraction apparatus of the present invention will be described.
  • the particle concentration / extraction apparatus according to the second embodiment is a modification of the configuration of the particle extraction unit 4 in the first embodiment. That is, the second embodiment is largely different from the first embodiment in that the particle extraction electrode is provided in the particle extraction unit without the light emitting element.
  • FIG. 6 shows a cross section of the main body in the middle in the thickness direction so that the internal configuration of the main body can be seen.
  • the particle concentration / extraction apparatus 200 according to the second embodiment includes a plate-shaped main body 10, a flow path 20 formed inside the main body 10, a particle concentration unit 30, and a particle extraction unit 40.
  • the main body 10 may be made of the same material as that of the main body 1 of the first embodiment. However, in this embodiment, the flow direction of the concentrated particles is not changed by the irradiation light of the light emitting element, so it is necessary to use a transparent material. There is no.
  • the electrodes 32A and 32B of the particle concentrating unit 30 are arranged in the center of the flow path 2 with a gap d.
  • the electrodes 32A and 32B are formed of a rectangular plate material as in the first embodiment, and the length direction of the rectangle is arranged along the flow direction of the liquid. Although these electrodes 32A and 32B are not shown in detail, they are connected to a power source so that an AC voltage is applied as in the first embodiment.
  • a particle extraction unit 40 is disposed downstream of the particle concentration unit 30. As shown in FIGS. 6 and 7, the particle extraction unit 40 includes rectangular plate-shaped first particle induction electrodes 8A and 8B, rectangular plate-shaped second particle induction electrodes 9A and 9B, It consists of branch channels 44A and 44B formed on the downstream side of the particle guide electrode, a particle outlet 6B and a liquid outlet 7B communicating with the branch channels 44A and 44B.
  • the first induction electrodes 8A and 8B of the particle extraction unit 40 shift the flow of fine particles concentrated by the electrodes 32A and 32B of the particle concentration unit 30 to one side with respect to the width direction of the flow path 2. It is.
  • the action of the first induction electrode is basically the same as the electrode of the particle concentrating part, and the flow of concentrated particles is moved or formed on the induction electrode by the alternating current electroosmosis effect, thereby causing a shift in the particle flow. .
  • the induction electrodes 8A and 8B do not accumulate the fine particles dispersed and flowing in the liquid, but only shift the accumulated particles, the length in the flow direction is the particle concentration unit. It may be shorter than the 3A electrode.
  • the second induction electrodes 9A and 9B are arranged on the downstream side of the first induction electrode. These second induction electrodes 9A and 9B are intended to further change the flow of the concentrated particles that have been changed in the width direction of the flow path by the first induction electrodes 9A and 9B in the direction of the side wall of the flow path. Then, the size of the second induction electrode is the same as that of the first induction electrode.
  • induction electrodes 8 and 9 are connected to an AC power source AC, respectively.
  • the induction electrodes 8 and 9 and the AC power supply AC also correspond to induction means in the particle concentration / extraction apparatus of the present invention.
  • the AC power source connected to these induction electrodes may generate the same AC voltage and frequency as the AC power source connected to the electrodes 31A and 31B described above, and preferably a single AC power source.
  • a synchronized AC voltage is preferably applied to the three sets of electrodes.
  • a liquid containing particles is injected from the inlet 5B and flows into the flow path 20 along the direction of the arrow a.
  • an AC electric field is applied to the electrode 32, the induction electrode 8, and the induction electrode 9.
  • an alternating current electroosmotic flow flows by the two electrodes 32 ⁇ / b> A and 32 ⁇ / b> B disposed in the flow channel 20 with a gap d sandwiching the center line of the flow channel 20. It occurs in a direction perpendicular to the direction.
  • the particles in the liquid flow in the direction of the flow a while being accumulated in a strip shape parallel to the flow a on the electrodes 32A and 32B by the alternating current electroosmotic flow.
  • Particles accumulated on the electrodes 32A and 32B in the particle concentrating unit 30 ride on the liquid flow a and move toward the particle extracting unit 40.
  • an alternating voltage synchronized with the first induction electrode 8 and the second induction electrode 9 is applied, an alternating current electroosmotic flow is also generated in the vicinity of the induction electrodes 8 and 9.
  • the fine particles flow while shifting the position of the flow in the width direction of the flow path for each electrode while accumulating on the electrode.
  • the concentrated fine particles are guided to the branch flow path 44A, the remaining liquid is guided to the branch flow path 44B, the concentrated fine particles are taken out from the particle outlet 6B, and the remaining liquid is taken out from the liquid outlet 7B.
  • the particle extraction unit 40 does not require a light irradiation apparatus, so that the configuration of the apparatus can be simplified. Moreover, since the flow path 20 is not irradiated with light, it is not always necessary to make the main body 10 of a transparent material. Therefore, the particle concentration / extraction apparatus 200 of the second embodiment has a range of selection of materials used for the main body in addition to the features of the particle extraction apparatus 100 of the first embodiment, compared with the particle extraction apparatus 100 of the first embodiment. It has the feature of becoming even wider.
  • Embodiment 3 Hereinafter, still another example of the particle concentration / extraction apparatus of the present invention will be described.
  • the particle concentration / extraction apparatus according to the third embodiment is an example in which the particle concentration unit also serves as the particle extraction unit in the second embodiment.
  • the particle concentration / extraction apparatus 300 includes a plate-shaped main body 11, a linear channel 21 formed inside the main body 11, and a particle concentration unit 31. And a particle extraction unit 41.
  • the particle concentration unit 31 also serves as the particle extraction unit 41.
  • the main body 11 may be made of the same material as that of the main body 1 of the first embodiment. However, in this embodiment, the flow direction of the concentrated particles is not changed by the irradiation light of the light emitting element, so it is necessary to use a transparent material. There is no.
  • one end of the channel 21 communicates with the inlet 5B for injecting a liquid containing particles
  • the other end of the channel 21 is a particle concentrating unit 31 (particle extracting unit 41).
  • the flow branches into three branch flow paths 45A, 45B, and 45C.
  • the branch channel 45A extends on the center line of the channel 21 and communicates with the particle outlet 6B.
  • the branch channels 45B and 45C are channels having a V-shaped planar shape formed so as to branch from the other end of the channel 21 so as to sandwich the branch channel 45A.
  • the branch channels 45B and 45C are combined at the end opposite to the channel 21 and communicate with the liquid outlet 6B.
  • the particle concentrating unit 31 includes an I-type electrode 35 ⁇ / b> A having an I-type planar shape along the channel 21 and the branch channel 45 ⁇ / b> A and a C-type plane.
  • a C-shaped electrode 35B having a shape is formed.
  • the I-type electrode 35A is formed so as to extend from the branch channel 45A toward the channel 21, and the C-type electrode 35B is formed so as to surround the tip of the I-type electrode 35A with a gap d therebetween.
  • the length of the I-type electrode 35A along the flow a direction can be 1 mm or more, preferably 2 to 10 mm.
  • the width of the I-type electrode 35A can be determined as appropriate according to the width of the flow path 21.
  • the width of the I-type electrode 35A is 100 to 400 ⁇ m, preferably 200. It can be set to ⁇ 350 ⁇ m, more preferably 250 to 350 ⁇ m.
  • the distance d between the I-type electrode 35A and the C-type electrode 35B is preferably from 1 to 100 ⁇ m, more preferably from 10 to 100 ⁇ m, and more preferably from 20 to 100 ⁇ m in view of ease of manufacture and the magnitude of the AC electroosmosis effect. Is particularly preferred.
  • the I-type electrode 35A and the C-type electrode 35B are connected to an AC power source AC.
  • electrodes 35C and 35D are disposed on both sides of the I-type electrode 35A along the flow path 21 as shown in FIG.
  • the electrode 35C and the electrode 35D may be electrically connected and connected to the AC power source AC together with the I-type electrode 35A.
  • a liquid containing particles is injected from the inlet 5B and flows into the flow path 20 along the direction of the arrow a.
  • the particles are dispersed throughout the flow channel 21 and the branch flow channels 45A, 45B, and 45C as shown in FIG.
  • the particles accumulated on the I-type electrode 35A are transported in the branch flow path 35A by the liquid flow a, and are taken out of the particle concentration / extraction device 300 from the particle outlet 6B.
  • the liquid separated from the particles flows through the branch flow paths 35B and 35C and is taken out from the liquid outlet 7B.
  • the particle concentration / extraction apparatus 300 according to the third embodiment has a configuration in which the particle concentration unit 31 also serves as the particle extraction unit 41 in addition to the features of the particle concentration / extraction apparatus 200 according to the second embodiment.
  • -It has the feature that a structure is further simplified compared with the extraction apparatus 200.
  • Embodiment 4 in the particle concentration / extraction apparatus of Embodiment 1, in order to send the liquid containing fine particles from the injection port 5 to the particle concentration unit 3, the liquid flows into the flow path 2 in the direction of the flow a in place of the pump or the syringe.
  • An example using an electroosmotic flow generated by applying a DC voltage will be described.
  • a positive electrode 36A is provided inside the injection port 5 and a negative electrode 36B is provided inside the particle outlet 6 as shown in FIG.
  • the positive electrode 36A is connected to the positive electrode of the DC power source 33C
  • the negative electrode 36B is connected to the negative electrode of the DC power source 33C.
  • the output voltage of the DC power source 33C is set so that the electric field strength along the flow path 2 is about 50 V / mm. Therefore, when the length of the flow path 2 is 40 mm, for example, the output voltage of the DC power supply 33C is set to about 2 kV.
  • the particle concentrating / extracting apparatus 400 of the fourth embodiment has the same configuration as the particle concentrating / extracting apparatus 100 of the first embodiment except for the above points.
  • Embodiment 4 The operation of the particle concentration / extraction apparatus 400 of Embodiment 4 will be described below.
  • a liquid containing fine particles is supplied to the inlet 5 in a state where a DC voltage from a DC power source is applied to the positive electrode 36A and the negative electrode 36B, an electrophoretic flow in the direction toward the particle outlet 6 inside the flow path 2 Occurs.
  • the liquid supplied to the inlet 5 is transferred to the particle concentrating unit 3 by this electrophoresis flow, and the fine particles of the liquid flow are concentrated on the electrodes 31A and 31B by the mechanism described in the first embodiment.
  • the fine particles concentrated on the electrodes 31A and 31B in the particle concentrating unit 3 are subjected to light pressure by the light emitted from the light emitting element 41 as described in the first embodiment, and are directed in the direction of the branch flow path 43A. It flows in a different manner and is taken out of the apparatus from the particle outlet 6.
  • the remaining liquid in which the fine particles are concentrated is taken out from the liquid outlet 7 through the branch channel 43B to the outside of the apparatus.
  • the particle concentration / extraction apparatus 400 of the fourth embodiment includes a pump or a syringe for feeding a liquid containing fine particles from the injection port 5 to the particle concentration unit 3. Has the feature that is no longer necessary.

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  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention porte sur un appareil pour la concentration et l'extraction de particules qui concentre des particules présentes dans un liquide qui contient des microparticules et extrait ces particules. De façon spécifique l'invention porte sur un appareil pour la concentration et l'extraction de particules comprenant : une unité principale (1) qui forme un trajet de circulation (2) pour le liquide contenant des microparticules ; au moins une paire d'électrodes (31) disposée le long de ce trajet de circulation (2) ; un moyen de concentration de particules (3) ayant une alimentation électrique en courant alternatif (33B) qui applique une tension alternative aux électrodes (31), ce qui augmente la concentration des microparticules circulant dans le trajet de circulation au voisinage des électrodes par un flux électroosmotique en courant alternatif ; au moins deux trajets de circulation en dérivation (43A, 43B) disposés dans le trajet de circulation du côté aval du moyen de concentration de particules (3) ; et une partie d'extraction de particules (4) ayant un moyen émettant de la lumière (41) disposé au voisinage de l'embranchement du trajet de circulation et dirigeant de la lumière sur l'une des dérivations du trajet de circulation à partir du côté de l'autre.
PCT/JP2011/055136 2010-09-07 2011-03-04 Appareil pour la concentration de particules et appareil pour la concentration et l'extraction de particules WO2012032802A1 (fr)

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JP2010-200246 2010-09-07
JP2010200246 2010-09-07

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WO2019020720A1 (fr) * 2017-07-25 2019-01-31 Koninklijke Philips N.V. Capteur de particules et procédé de détection de particules
CN110918139A (zh) * 2018-09-20 2020-03-27 北京怡天佳瑞科技有限公司 微流控芯片、含有该微流控芯片的装置及样本浓缩的方法

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