WO2012030072A3 - Root hair-specific expression promoter derived from expb1 gene of barley and use thereof - Google Patents

Root hair-specific expression promoter derived from expb1 gene of barley and use thereof Download PDF

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Publication number
WO2012030072A3
WO2012030072A3 PCT/KR2011/005563 KR2011005563W WO2012030072A3 WO 2012030072 A3 WO2012030072 A3 WO 2012030072A3 KR 2011005563 W KR2011005563 W KR 2011005563W WO 2012030072 A3 WO2012030072 A3 WO 2012030072A3
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WO
WIPO (PCT)
Prior art keywords
gene
expb1
barley
specific expression
root
Prior art date
Application number
PCT/KR2011/005563
Other languages
French (fr)
Korean (ko)
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WO2012030072A2 (en
Inventor
조형택
Original Assignee
서울대학교산학협력단
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Application filed by 서울대학교산학협력단 filed Critical 서울대학교산학협력단
Publication of WO2012030072A2 publication Critical patent/WO2012030072A2/en
Publication of WO2012030072A3 publication Critical patent/WO2012030072A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • C12N15/8227Root-specific
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a root hair-specific expression promoter derived from EXPB1 gene of barley and to a use thereof, and more particularly, to a root hair-specific expression promoter derived from EXPB1 gene of barley, to a root-specific plant expression vector including the promoter, to a plant body which is transformed into the vector, to a method for root-specifically expressing a foreign gene in the transformed plant body including a step of recombining the foreign gene with the vector, to a transformed plant body prepared by the method wherein a foreign gene is root-specifically expressed, and to a seed of the transformed plant body.
PCT/KR2011/005563 2010-08-30 2011-07-28 Root hair-specific expression promoter derived from expb1 gene of barley and use thereof WO2012030072A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0083817 2010-08-30
KR1020100083817A KR101210306B1 (en) 2010-08-30 2010-08-30 Root hair-specific expression promoter from Hordeum vulgare EXPB1 gene and uses thereof

Publications (2)

Publication Number Publication Date
WO2012030072A2 WO2012030072A2 (en) 2012-03-08
WO2012030072A3 true WO2012030072A3 (en) 2012-06-21

Family

ID=45773338

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/005563 WO2012030072A2 (en) 2010-08-30 2011-07-28 Root hair-specific expression promoter derived from expb1 gene of barley and use thereof

Country Status (2)

Country Link
KR (1) KR101210306B1 (en)
WO (1) WO2012030072A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101485260B1 (en) * 2013-10-28 2015-01-22 경희대학교 산학협력단 Root-specific promoter, Expression vector comprising the same, transformed plants thereby and method for preparation thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7217861B2 (en) * 2003-03-27 2007-05-15 Seoul National University Industry Foundation Root-specific expansin gene regulating root growth and obstacle-touching stress resistance in the plant
KR20100065808A (en) * 2008-12-09 2010-06-17 서울대학교산학협력단 Regulation method of plant size and the plant thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100935339B1 (en) 2003-06-21 2010-01-06 중앙대학교 산학협력단 Expansin 5 protein of Arabidopsis thaliana, transgenic plant thereof and its use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7217861B2 (en) * 2003-03-27 2007-05-15 Seoul National University Industry Foundation Root-specific expansin gene regulating root growth and obstacle-touching stress resistance in the plant
KR20100065808A (en) * 2008-12-09 2010-06-17 서울대학교산학협력단 Regulation method of plant size and the plant thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DONG WOOK KIM ET AL.: "Functional Conservation of a Root Hair Cell-Specific cis-Element in Angiosperms with Different Root Hair Distribution Patterns", THE PLANT CELL, vol. 18, November 2006 (2006-11-01), pages 2958 - 2970 *
MIROSLAW KWASNIEWSKI ET AL.: "Molecular Cloning and Characterization of beta-Expansin Gene Related to Root Hair Formation in Barley", PLANT PHYSIOLOGY, vol. 141, July 2006 (2006-07-01), pages 1149 - 1158 *
SU-KYUNG WON ET AL.: "Root Hair-Specific EXPANSIN B Genes Have Been Selected for Graminaceae Root Hairs", MOLECULES & CELLS, vol. 30, 27 August 2010 (2010-08-27), pages 369 - 376 *
YI LEE ET AL.: "Expression of beta-Expansins Is Correlated with Internodal Elongation in Deepwater Rice", PLANT PHYSIOLOGY, vol. 127, October 2001 (2001-10-01), pages 645 - 654 *

Also Published As

Publication number Publication date
KR101210306B1 (en) 2012-12-10
KR20120020290A (en) 2012-03-08
WO2012030072A2 (en) 2012-03-08

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